Mercurial > repos > devteam > fastq_combiner
changeset 0:82f441097692 draft
Imported from capsule None
author | devteam |
---|---|
date | Thu, 23 Jan 2014 12:31:59 -0500 |
parents | |
children | 02c665f7ac2b |
files | fastq_combiner.py fastq_combiner.xml test-data/combine_phiX_out_1.fastqcssanger test-data/combine_phiX_out_2.fastqcssanger test-data/fastq_combiner_in_1.fasta test-data/fastq_combiner_in_1.qual454 test-data/fastq_combiner_no_qual_ascii_out_1.fastqcssanger test-data/fastq_combiner_no_qual_decimal_out_1.fastqsanger test-data/s2fq_phiX.csfasta test-data/s2fq_phiX.qualsolid test-data/wrapping_as_sanger.fastqsanger test-data/wrapping_as_sanger_decimal.fastqsanger tool_dependencies.xml |
diffstat | 13 files changed, 2511 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fastq_combiner.py Thu Jan 23 12:31:59 2014 -0500 @@ -0,0 +1,49 @@ +#Dan Blankenberg +import sys, os, shutil +from galaxy_utils.sequence.fastq import fastqWriter, fastqSequencingRead, fastqCombiner, fastqFakeFastaScoreReader +from galaxy_utils.sequence.fasta import fastaReader, fastaNamedReader + +def main(): + #Read command line arguments + fasta_filename = sys.argv[1] + fasta_type = sys.argv[2] or 'fasta' #should always be fasta or csfasta? what if txt? + qual_filename = sys.argv[3] + qual_type = sys.argv[4] or 'qualsanger' #qual454 qualsolid + output_filename = sys.argv[5] + force_quality_encoding = sys.argv[6] + if force_quality_encoding == 'None': + force_quality_encoding = None + + format = 'sanger' + if fasta_type == 'csfasta' or qual_type == 'qualsolid': + format = 'cssanger' + elif qual_type == 'qualsolexa': + format = 'solexa' + elif qual_type == 'qualillumina': + format = 'illumina' + + out = fastqWriter( open( output_filename, 'wb' ), format = format, force_quality_encoding = force_quality_encoding ) + if qual_filename == 'None': + qual_input = fastqFakeFastaScoreReader( format, quality_encoding = force_quality_encoding ) + else: + qual_input = fastaNamedReader( open( qual_filename, 'rb' ) ) + + fastq_combiner = fastqCombiner( format ) + i = None + skip_count = 0 + for i, sequence in enumerate( fastaReader( open( fasta_filename, 'rb' ) ) ): + quality = qual_input.get( sequence ) + if quality: + fastq_read = fastq_combiner.combine( sequence, quality ) + out.write( fastq_read ) + else: + skip_count += 1 + out.close() + if i is None: + print "Your file contains no valid FASTA sequences." + else: + print qual_input.has_data() + print 'Combined %s of %s sequences with quality scores (%.2f%%).' % ( i - skip_count + 1, i + 1, float( i - skip_count + 1 ) / float( i + 1 ) * 100.0 ) + +if __name__ == "__main__": + main()
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fastq_combiner.xml Thu Jan 23 12:31:59 2014 -0500 @@ -0,0 +1,80 @@ +<tool id="fastq_combiner" name="Combine FASTA and QUAL" version="1.0.1"> + <description>into FASTQ</description> + <requirements> + <requirement type="package" version="1.0.0">galaxy_sequence_utils</requirement> + </requirements> + <command interpreter="python">fastq_combiner.py '$fasta_file' '${fasta_file.extension}' '$qual_file' '${qual_file.extension}' '$output_file' '$force_quality_encoding'</command> + <inputs> + <param name="fasta_file" type="data" format="fasta,csfasta" label="FASTA File" /> + <param name="qual_file" type="data" format="qual" label="Quality Score File" optional="True" /> + <param name="force_quality_encoding" type="select" label="Force Quality Score encoding"> + <option value="None">Use Source Encoding</option> + <option value="ascii" selected="True">ASCII</option> + <option value="decimal">Decimal</option> + </param> + </inputs> + <outputs> + <data name="output_file" format="fastqsanger"> + <change_format> + <when input_dataset="fasta_file" attribute="extension" value="csfasta" format="fastqcssanger" /> + <when input_dataset="qual_file" attribute="extension" value="qualsolid" format="fastqcssanger" /> + <when input_dataset="qual_file" attribute="extension" value="qualsolexa" format="fastqsolexa" /> + <when input_dataset="qual_file" attribute="extension" value="qualillumina" format="fastqillumina" /> + </change_format> + </data> + </outputs> + <tests> + <test> + <param name="fasta_file" value="s2fq_phiX.csfasta" ftype="csfasta" /> + <param name="qual_file" value="s2fq_phiX.qualsolid" ftype="qualsolid" /> + <param name="force_quality_encoding" value="None" /> + <output name="output_file" file="combine_phiX_out_1.fastqcssanger" /> + </test> + <test> + <param name="fasta_file" value="s2fq_phiX.csfasta" ftype="csfasta" /> + <param name="qual_file" value="s2fq_phiX.qualsolid" ftype="qualsolid" /> + <param name="force_quality_encoding" value="ascii" /> + <output name="output_file" file="combine_phiX_out_2.fastqcssanger" /> + </test> + <test> + <param name="fasta_file" value="fastq_combiner_in_1.fasta" ftype="fasta" /> + <param name="qual_file" value="fastq_combiner_in_1.qual454" ftype="qual454" /> + <param name="force_quality_encoding" value="None" /> + <output name="output_file" file="wrapping_as_sanger.fastqsanger" /> + </test> + <test> + <param name="fasta_file" value="fastq_combiner_in_1.fasta" ftype="fasta" /> + <param name="qual_file" value="fastq_combiner_in_1.qual454" ftype="qual454" /> + <param name="force_quality_encoding" value="decimal" /> + <output name="output_file" file="wrapping_as_sanger_decimal.fastqsanger" /> + </test> + <test> + <param name="fasta_file" value="fastq_combiner_in_1.fasta" ftype="fasta" /> + <param name="qual_file" /> + <param name="force_quality_encoding" value="decimal" /> + <output name="output_file" file="fastq_combiner_no_qual_decimal_out_1.fastqsanger" /> + </test> + <test> + <param name="fasta_file" value="s2fq_phiX.csfasta" ftype="csfasta" /> + <param name="qual_file" /> + <param name="force_quality_encoding" value="ascii" /> + <output name="output_file" file="fastq_combiner_no_qual_ascii_out_1.fastqcssanger" /> + </test> + </tests> + <help> +**What it does** + +This tool joins a FASTA file to a Quality Score file, creating a single FASTQ block for each read. + +Specifying a set of quality scores is optional; when not provided, the output will be fastqsanger or fastqcssanger (when a csfasta is provided) with each quality score being the maximal allowed value (93). + +Use this tool, for example, to convert 454-type output to FASTQ. + +------ + +**Citation** + +If you use this tool, please cite `Blankenberg D, Gordon A, Von Kuster G, Coraor N, Taylor J, Nekrutenko A; Galaxy Team. Manipulation of FASTQ data with Galaxy. Bioinformatics. 2010 Jul 15;26(14):1783-5. <http://www.ncbi.nlm.nih.gov/pubmed/20562416>`_ + + </help> +</tool>
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/combine_phiX_out_2.fastqcssanger Thu Jan 23 12:31:59 2014 -0500 @@ -0,0 +1,576 @@ +@1831_573_1004_F3 +T00030133312212111300011021310132222 ++ +%><C&&9952+C>5<.?<79,=42<292:<(9/-7 +@1831_573_1050_F3 +T03330322230322112131010221102122113 ++ +);@@17?@=>7??@A8?==@4A?A4)&+.'&+'1, +@1831_573_1067_F3 +T00023032023103330112220321200200002 ++ +.++#%(',%/$,+&1#&),)&+'*'*%#$&#%('$ +@1831_573_1219_F3 +T11211130300300301021212330201121310 ++ +@@@=4/+)5)408?'665>*/5?<61';<3,:,5- +@1831_573_1242_F3 +T02132003121011302100130302112221121 ++ +>>>::>17=A5?@@=;7A=;2.60>82<8=74+;; +@1831_573_1333_F3 +T00200312330110101013212313222303112 ++ +)='@#%3=.>)/34*117,,/6-4+.9742456<) +@1831_573_1362_F3 +T21203131001102231121211101111321131 ++ +CB@@?@@?@C@BA?@>@<@8A@?@'.8'?17:,+? +@1831_573_1448_F3 +T23101211223113320132212331313312022 ++ +77=A)?,.@A@@:@@<=/7)@<-#8662%9613&+ +@1831_573_1490_F3 +T31312310323301210002210123101021011 ++ 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+T21001132013000122220301213221213010 ++ +%$>2,(&?4?(,@:<&,@>?$&:8A%%=0.%,597 +@1831_576_1289_F3 +T03021210023110200323310302013121203 ++ +@9<>1B@@;7@@663==28,5':8<<,.=2>>.50 +@1831_576_1329_F3 +T01100302102020113003022000120002100 ++ +<>&0,870/A@@/5.;=;:'&@3'&$$%,+#($&2 +@1831_576_1367_F3 +T12231310311233110031222013332011023 ++ +??@@<>@@=@<@<=@>@??9:?<=>=<8;59@787 +@1831_576_1416_F3 +T33021233100123120313103133211203221 ++ ++';?..1.<@'.=+6.5?7<0-?7;(%=>56.98@ +@1831_576_1461_F3 +T32022221221112233100210223002100100 ++ +#1&74#(#(&##$#,'###%#%%#*#&%%##%%%' +@1831_576_1605_F3 +T30232100103132133321330310210101221 ++ +6<@C@@>1;@:;<<@@9@???9<3?5-21=4877, +@1831_576_1664_F3 +T31212101001312110320301201002011120 ++ +@@?>?@=A:@@>@='=<>=).>=9*8)(7#/++;) +@1831_576_1671_F3 +T22313332300211322113223102231322313 ++ +@@?@5>@@8&8?25#&)&5&&)15&&,&4%&,&6% +@1831_576_1729_F3 +T11233312313010012320101302101023030 ++ +@?@?<?<@>A?/==?>?7>?@8<?@>99;><+0=> +@1831_576_1880_F3 +T13032121323320213301001310130212003 ++ +0515/792,:,7/%/05,%$):+#8%2(1754))3 +@1831_576_1982_F3 +T00032312310201201333221212000011030 ++ +,@@?@,A><@9@=?@9B=8<6@@6@2<A?>.7<+@ +@1831_576_1987_F3 +T30022313313231221213220132001011320 ++ +)##()(#&##)%)/-#%$11#%3>'##&$,#$$'+ +@1831_576_2014_F3 +T31123201010100321122111102113021003 ++ +@@@9,B29-5>'?,+?79+/A';'2@'5&/9,6&1 +@1831_576_2028_F3 +T20131211210311112023201213120201100 ++ +5<>9>5'2(&707.8#&&39,0%7/#(#,*%&5*+ +@1831_577_40_F3 +T11111212330120012020200031313303003 ++ +92/4('.')(<*?#$)%&<,/39<(.2,+<=@611 +@1831_577_119_F3 +T33111010021103320103213121313000102 ++ +9>?C11)-1/)#;#/,850*+.+$$5550+%-.40 +@1831_577_133_F3 +T33213323012231300122223032223331322 ++ +/4@>>?=1?:+>@07@@><>@*<+1@15)96'2$) +@1831_577_255_F3 +T00332022110020300332022020202002232 ++ +7<=87@>69%**#&#-+$.#&&#+$-+%&%.,%5# +@1831_577_281_F3 +T03032301231212301013112222111210000 ++ +7-:B;8@=;>7@>4?=?+659?;5<7?;9@8(>:? +@1831_577_288_F3 +T01031120221303100221230021013201130 ++ +5;/79??&=B::298*6.7/+4&21,7,6?.7#'6 +@1831_577_322_F3 +T12003213220230103303201000130312202 ++ +9=;>4506;255464-<#7+194&2<?65968)7/ +@1831_577_362_F3 +T31203302330110131230331210121110220 ++ +62B?:@?@<?958=,3:,90:&'-99,6<5.($+6 +@1831_577_382_F3 +T32312123033111120321303230201332100 ++ +12957/:1))=76(*24;,3+:<.&.&-=1=2/5* +@1831_577_464_F3 +T13020221011130013102221333131203302 ++ +3=<7/<826)>#.'&4204+5#/041.7*91&756 +@1831_577_488_F3 +T13200302330322110200323132101120301 ++ +:48.,*>6566<?8=<=<2>6;94>;=9>@8924@ +@1831_577_511_F3 +T32232133031023313331312220133230333 ++ +#7/+&$:<7%6,$$%'%/+)#$7((&*3>16'0/+ +@1831_577_545_F3 +T00112131333222303222210031322103233 ++ +>><<9?<>?A<481@<@8==@76/61<95.5988- +@1831_577_559_F3 +T32321101303233120102011130022122002 ++ +.<@;??@>@?/2<.@1=>12=61/;=?.&2+92)' +@1831_577_562_F3 +T32331101301233110121000220031120031 ++ +#((&*&0%)1%)#($2-,***%/-,,))&,-.1'* +@1831_577_637_F3 +T22113312122202103031023120301031110 ++ +8-,4#>:-6+:8,&(5;3=0>7=68&1/9&'?;4, +@1831_577_641_F3 +T13031301101121223221212020032131113 ++ +&47;/':A;;5?:72,(=),#*?+.#&7$8#%7/' +@1831_577_692_F3 +T01122320200330103121202301211100220 ++ +@B,@?@B(@A?@+@@>@+?=>@'=<@<=<9=?75<
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastq_combiner_in_1.fasta Thu Jan 23 12:31:59 2014 -0500 @@ -0,0 +1,14 @@ +>SRR014849.50939 EIXKN4201BA2EC length=135 +GAAATTTCAGGGCCACCTTTTTTTT +GATAGAATAATGGAGAAAATTAAAAGCTGTACATATACCAATGAACAATAAATCAATACATAAAAAAGGA +GAAGTTGGAACCGAAAGGGTTTGAATTCAAACCCTTTCGG +>SRR014849.110027 EIXKN4201APUB0 length=131 +CTTCAAATGATTC +CGGGACTGTTGGAACCGAAAGGGTTTGAATTCAAACCC +TTTTCGGTTCCAACTCGCCGTCCGAATAATCCGTTCAAAATCTTGGCCTGTCAAAACGACTTTACGAC +CAGAACGATCCG +>SRR014849.203935 EIXKN4201B4HU6 length=144 +AACCCGTCCCA +TCAAAGATTTTGGTTGGAACCCGAAAGGGTTTTGAATTCAAACC +CCTTTCGGTTCCAACTATTCAATTGTTTAACTTTTTT +TAAATTGATGGTCTGTTGGACCATTTGTAATAATCCCCATCGGAATTTCTTT
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastq_combiner_in_1.qual454 Thu Jan 23 12:31:59 2014 -0500 @@ -0,0 +1,22 @@ +>SRR014849.110027 EIXKN4201APUB0 length=131 +=B::@<':=5A9?7EA0:=<<?6@7<3?5< +@;%D?-B=)::0=4<D? +-EA/D@2";B;B: +B:A9;;=<B;;<B;<B;<B;:A;<A;8FB7 ++=<B;B:A9<1:=FB6(<=<<EA0956;<2 +==A8===:@8= +>SRR014849.203935 EIXKN4201B4HU6 length=144 +A;@;%75?:#<<9EA1;=EA3%B;B;A;B; +@;%9EA1EA1EA3%<B;A;8EA0D@ +3$EA1 +=B;A;B;B;:=:B;:B:A9:EA0A9<FA81 ++&"D?-B;4<::/<;=:A98-5?6=C>+8< +<3;=4:DA3%<;=8-9.A=):B=* +>SRR014849.50939 EIXKN4201BA2EC length=135 +;C?-EA/=<EA/B;<B;D>60,)%"<=:5< +@8<B;=B;<;E +A4'@8FB6*<:=<<===<= +;=B:A9<<B;=B;=EA0:<B:<<=<<FA81 ++$?6;<A9=<3>5@7@8<A<(B=*A=)<<2 +?57B=*B=*D?-:=4 +
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastq_combiner_no_qual_ascii_out_1.fastqcssanger Thu Jan 23 12:31:59 2014 -0500 @@ -0,0 +1,576 @@ +@1831_573_1004_F3 +T00030133312212111300011021310132222 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1050_F3 +T03330322230322112131010221102122113 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1067_F3 +T00023032023103330112220321200200002 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1219_F3 +T11211130300300301021212330201121310 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1242_F3 +T02132003121011302100130302112221121 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1333_F3 +T00200312330110101013212313222303112 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1362_F3 +T21203131001102231121211101111321131 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1448_F3 +T23101211223113320132212331313312022 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1490_F3 +T31312310323301210002210123101021011 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1523_F3 +T10322001220012223202202222001230222 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1578_F3 +T21202302100010020121100311022120111 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1647_F3 +T10222233301013033120132223202022123 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1684_F3 +T13310013212312012302121010221231123 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1769_F3 +T33220123030232212032021032302233131 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1853_F3 +T11000012111222211310103212122102331 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1943_F3 +T20300123032210232001222122001132111 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_573_1977_F3 +T22212302221310332321002303112011311 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_109_F3 +T13122332123301331032220222133301033 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_148_F3 +T01200113123030012202302312200010231 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_185_F3 +T21123333211302300321312212102123121 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_243_F3 +T30221011230013102201033131203302330 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_257_F3 +T00301133110002100302003000000102301 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_293_F3 +T23213210003000103010211331300320130 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_389_F3 +T21032213032101122333230212301312020 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_575_F3 +T33313322100212102033032123311211302 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_592_F3 +T33103330110123102223122023103310330 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_617_F3 +T20021031221222021210021322200223211 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_725_F3 +T32010020322130330333010031120313210 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_734_F3 +T31132301200020012302210322213222222 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_824_F3 +T30212100033032123311211302122020013 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_959_F3 +T11212130220131221111002020123311211 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_1062_F3 +T30112230030300221001032033012211012 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_1092_F3 +T02013221200031031212200000111130310 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_1103_F3 +T20313113203302010303131123021310121 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_1116_F3 +T21011310123202303021021112021231011 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_1194_F3 +T23303101033322220312200222013013312 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_1204_F3 +T21330132231321322010303023221203200 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_1306_F3 +T10332133020311023221213100301001220 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_1387_F3 +T12301331310032132101301303230121111 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_574_1431_F3 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++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_577_322_F3 +T12003213220230103303201000130312202 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_577_362_F3 +T31203302330110131230331210121110220 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_577_382_F3 +T32312123033111120321303230201332100 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_577_464_F3 +T13020221011130013102221333131203302 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_577_488_F3 +T13200302330322110200323132101120301 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_577_511_F3 +T32232133031023313331312220133230333 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_577_545_F3 +T00112131333222303222210031322103233 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_577_559_F3 +T32321101303233120102011130022122002 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_577_562_F3 +T32331101301233110121000220031120031 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +@1831_577_637_F3 +T22113312122202103031023120301031110 ++ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastq_combiner_no_qual_decimal_out_1.fastqsanger Thu Jan 23 12:31:59 2014 -0500 @@ -0,0 +1,12 @@ +@SRR014849.50939 EIXKN4201BA2EC length=135 +GAAATTTCAGGGCCACCTTTTTTTTGATAGAATAATGGAGAAAATTAAAAGCTGTACATATACCAATGAACAATAAATCAATACATAAAAAAGGAGAAGTTGGAACCGAAAGGGTTTGAATTCAAACCCTTTCGG ++ +93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 +@SRR014849.110027 EIXKN4201APUB0 length=131 +CTTCAAATGATTCCGGGACTGTTGGAACCGAAAGGGTTTGAATTCAAACCCTTTTCGGTTCCAACTCGCCGTCCGAATAATCCGTTCAAAATCTTGGCCTGTCAAAACGACTTTACGACCAGAACGATCCG ++ +93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 +@SRR014849.203935 EIXKN4201B4HU6 length=144 +AACCCGTCCCATCAAAGATTTTGGTTGGAACCCGAAAGGGTTTTGAATTCAAACCCCTTTCGGTTCCAACTATTCAATTGTTTAACTTTTTTTAAATTGATGGTCTGTTGGACCATTTGTAATAATCCCCATCGGAATTTCTTT ++ +93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93 93
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/s2fq_phiX.csfasta Thu Jan 23 12:31:59 2014 -0500 @@ -0,0 +1,288 @@ +>1831_573_1004_F3 +T00030133312212111300011021310132222 +>1831_573_1050_F3 +T03330322230322112131010221102122113 +>1831_573_1067_F3 +T00023032023103330112220321200200002 +>1831_573_1219_F3 +T11211130300300301021212330201121310 +>1831_573_1242_F3 +T02132003121011302100130302112221121 +>1831_573_1333_F3 +T00200312330110101013212313222303112 +>1831_573_1362_F3 +T21203131001102231121211101111321131 +>1831_573_1448_F3 +T23101211223113320132212331313312022 +>1831_573_1490_F3 +T31312310323301210002210123101021011 +>1831_573_1523_F3 +T10322001220012223202202222001230222 +>1831_573_1578_F3 +T21202302100010020121100311022120111 +>1831_573_1647_F3 +T10222233301013033120132223202022123 +>1831_573_1684_F3 +T13310013212312012302121010221231123 +>1831_573_1769_F3 +T33220123030232212032021032302233131 +>1831_573_1853_F3 +T11000012111222211310103212122102331 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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/wrapping_as_sanger.fastqsanger Thu Jan 23 12:31:59 2014 -0500 @@ -0,0 +1,12 @@ +@SRR014849.50939 EIXKN4201BA2EC length=135 +GAAATTTCAGGGCCACCTTTTTTTTGATAGAATAATGGAGAAAATTAAAAGCTGTACATATACCAATGAACAATAAATCAATACATAAAAAAGGAGAAGTTGGAACCGAAAGGGTTTGAATTCAAACCCTTTCGG ++ +;C?-EA/=<EA/B;<B;D>60,)%"<=:5<@8<B;=B;<;EA4'@8FB6*<:=<<===<=;=B:A9<<B;=B;=EA0:<B:<<=<<FA81+$?6;<A9=<3>5@7@8<A<(B=*A=)<<2?57B=*B=*D?-:=4 +@SRR014849.110027 EIXKN4201APUB0 length=131 +CTTCAAATGATTCCGGGACTGTTGGAACCGAAAGGGTTTGAATTCAAACCCTTTTCGGTTCCAACTCGCCGTCCGAATAATCCGTTCAAAATCTTGGCCTGTCAAAACGACTTTACGACCAGAACGATCCG ++ +=B::@<':=5A9?7EA0:=<<?6@7<3?5<@;%D?-B=)::0=4<D?-EA/D@2";B;B:B:A9;;=<B;;<B;<B;<B;:A;<A;8FB7+=<B;B:A9<1:=FB6(<=<<EA0956;<2==A8===:@8= +@SRR014849.203935 EIXKN4201B4HU6 length=144 +AACCCGTCCCATCAAAGATTTTGGTTGGAACCCGAAAGGGTTTTGAATTCAAACCCCTTTCGGTTCCAACTATTCAATTGTTTAACTTTTTTTAAATTGATGGTCTGTTGGACCATTTGTAATAATCCCCATCGGAATTTCTTT ++ +A;@;%75?:#<<9EA1;=EA3%B;B;A;B;@;%9EA1EA1EA3%<B;A;8EA0D@3$EA1=B;A;B;B;:=:B;:B:A9:EA0A9<FA81+&"D?-B;4<::/<;=:A98-5?6=C>+8<<3;=4:DA3%<;=8-9.A=):B=*
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/wrapping_as_sanger_decimal.fastqsanger Thu Jan 23 12:31:59 2014 -0500 @@ -0,0 +1,12 @@ +@SRR014849.50939 EIXKN4201BA2EC length=135 +GAAATTTCAGGGCCACCTTTTTTTTGATAGAATAATGGAGAAAATTAAAAGCTGTACATATACCAATGAACAATAAATCAATACATAAAAAAGGAGAAGTTGGAACCGAAAGGGTTTGAATTCAAACCCTTTCGG ++ +26 34 30 12 36 32 14 28 27 36 32 14 33 26 27 33 26 35 29 21 15 11 8 4 1 27 28 25 20 27 31 23 27 33 26 28 33 26 27 26 36 32 19 6 31 23 37 33 21 9 27 25 28 27 27 28 28 28 27 28 26 28 33 25 32 24 27 27 33 26 28 33 26 28 36 32 15 25 27 33 25 27 27 28 27 27 37 32 23 16 10 3 30 21 26 27 32 24 28 27 18 29 20 31 22 31 23 27 32 27 7 33 28 9 32 28 8 27 27 17 30 20 22 33 28 9 33 28 9 35 30 12 25 28 19 +@SRR014849.110027 EIXKN4201APUB0 length=131 +CTTCAAATGATTCCGGGACTGTTGGAACCGAAAGGGTTTGAATTCAAACCCTTTTCGGTTCCAACTCGCCGTCCGAATAATCCGTTCAAAATCTTGGCCTGTCAAAACGACTTTACGACCAGAACGATCCG ++ +28 33 25 25 31 27 6 25 28 20 32 24 30 22 36 32 15 25 28 27 27 30 21 31 22 27 18 30 20 27 31 26 4 35 30 12 33 28 8 25 25 15 28 19 27 35 30 12 36 32 14 35 31 17 1 26 33 26 33 25 33 25 32 24 26 26 28 27 33 26 26 27 33 26 27 33 26 27 33 26 25 32 26 27 32 26 23 37 33 22 10 28 27 33 26 33 25 32 24 27 16 25 28 37 33 21 7 27 28 27 27 36 32 15 24 20 21 26 27 17 28 28 32 23 28 28 28 25 31 23 28 +@SRR014849.203935 EIXKN4201B4HU6 length=144 +AACCCGTCCCATCAAAGATTTTGGTTGGAACCCGAAAGGGTTTTGAATTCAAACCCCTTTCGGTTCCAACTATTCAATTGTTTAACTTTTTTTAAATTGATGGTCTGTTGGACCATTTGTAATAATCCCCATCGGAATTTCTTT ++ +32 26 31 26 4 22 20 30 25 2 27 27 24 36 32 16 26 28 36 32 18 4 33 26 33 26 32 26 33 26 31 26 4 24 36 32 16 36 32 16 36 32 18 4 27 33 26 32 26 23 36 32 15 35 31 18 3 36 32 16 28 33 26 32 26 33 26 33 26 25 28 25 33 26 25 33 25 32 24 25 36 32 15 32 24 27 37 32 23 16 10 5 1 35 30 12 33 26 19 27 25 25 14 27 26 28 25 32 24 23 12 20 30 21 28 34 29 10 23 27 27 18 26 28 19 25 35 32 18 4 27 26 28 23 12 24 13 32 28 8 25 33 28 9
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Thu Jan 23 12:31:59 2014 -0500 @@ -0,0 +1,6 @@ +<?xml version="1.0"?> +<tool_dependency> + <package name="galaxy_sequence_utils" version="1.0.0"> + <repository changeset_revision="195699b1562a" name="package_galaxy_utils_1_0" owner="devteam" prior_installation_required="False" toolshed="http://testtoolshed.g2.bx.psu.edu" /> + </package> +</tool_dependency>