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annotate bwa_macros.xml @ 17:23e88ff6c494 draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit e953b3b7dac6cbe9509fdc673907a7c2c7183180
author iuc
date Wed, 19 Mar 2025 17:24:58 +0000
parents ceed4b724f0b
children 52f5f04041f2
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1 <macros>
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2 <import>read_group_macros.xml</import>
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4 <token name="@TOOL_VERSION@">0.7.18</token>
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6 <token name="@pipefail@"><![CDATA[set -o | grep -q pipefail && set -o pipefail;]]></token>
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8 <token name="@set_rg_string@">
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9 #set $rg_string = "@RG\\tID:" + str($rg_id)
cbc665adcde4 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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10 #set $rg_string += $format_read_group("\\tSM:", $rg_sm)
cbc665adcde4 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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11 #set $rg_string += $format_read_group("\\tPL:", $rg_pl)
cbc665adcde4 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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12 #set $rg_string += $format_read_group("\\tLB:", $rg_lb)
cbc665adcde4 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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13 #set $rg_string += $format_read_group("\\tCN:", $rg_cn)
cbc665adcde4 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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14 #set $rg_string += $format_read_group("\\tDS:", $rg_ds)
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15 #set $rg_string += $format_read_group("\\tDT:", $rg_dt)
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16 #set $rg_string += $format_read_group("\\tFO:", $rg_fo)
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17 #set $rg_string += $format_read_group("\\tKS:", $rg_ks)
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18 #set $rg_string += $format_read_group("\\tPG:", $rg_pg)
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19 #set $rg_string += $format_read_group("\\tPI:", $rg_pi)
cbc665adcde4 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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20 #set $rg_string += $format_read_group("\\tPU:", $rg_pu)
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21 </token>
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23 <token name="@set_reference_fasta_filename@"><![CDATA[
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24 #if str( $reference_source.reference_source_selector ) == "history":
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25 #set $reference_fasta_filename = "localref.fa"
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26 ln -s '${reference_source.ref_file}' '${reference_fasta_filename}' &&
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27 bwa index
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28 #if str($reference_source.index_a) != 'auto'
b4dfb5470bf3 planemo upload for repository https://github.com/galaxyproject/tools-devteam/tree/master/tools/bwa commit 13b3ab10ff1a2e4be39b44f885df6bbe7c23099a
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29 -a ${reference_source.index_a}
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30 #end if
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cbc665adcde4 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/bwa commit c355891532cecaab6b3288a148a6b3bcb5973396
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31 '${reference_fasta_filename}' &&
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32 #else:
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33 #set $reference_fasta_filename = str( $reference_source.ref_file.fields.path )
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34 #end if
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35 ]]></token>
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36
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37 <xml name="requirements">
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38 <requirements>
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39 <requirement type="package" version="@TOOL_VERSION@">bwa</requirement>
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40 <requirement type="package" version="1.20">samtools</requirement>
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41 </requirements>
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42 </xml>
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43
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44 <xml name="bio_tools">
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45 <xrefs>
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46 <xref type="bio.tools">bwa</xref>
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47 </xrefs>
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48 </xml>
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49
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50 <xml name="stdio">
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51 <stdio>
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52 <exit_code range="1:" />
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53 <exit_code range=":-1" />
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54 <regex match="Error:" />
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55 <regex match="Exception:" />
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56 <regex match="\[bns_restore_core\] Parse error reading" />
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57 </stdio>
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58 </xml>
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59
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60 <macro name="reference_source_conditional">
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61 <conditional name="reference_source">
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62 <param name="reference_source_selector" type="select" label="Will you select a reference genome from your history or use a built-in index?" help="Built-ins were indexed using default options. See `Indexes` section of help below">
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63 <option value="cached">Use a built-in genome index</option>
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64 <option value="history">Use a genome from history and build index</option>
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65 </param>
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66 <when value="cached">
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67 <param name="ref_file" type="select" label="Using reference genome" help="Select genome from the list">
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68 <options from_data_table="bwa_mem_indexes">
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69 <filter type="sort_by" column="2" />
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70 <validator type="no_options" message="No indexes are available" />
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71 </options>
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72 <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/>
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73 </param>
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74 </when>
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75 <when value="history">
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76 <param name="ref_file" type="data" format="fasta" label="Use the following dataset as the reference sequence" help="You can upload a FASTA sequence to the history and use it as reference" />
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77 <param name="index_a" type="select" label="Algorithm for constructing the BWT index" help="(-a)">
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78 <option value="auto">Auto. Let BWA decide the best algorithm to use</option>
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79 <option value="is">IS linear-time algorithm for constructing suffix array. It requires 5.37N memory where N is the size of the database. IS is moderately fast, but does not work with database larger than 2GB</option>
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80 <option value="bwtsw">BWT-SW algorithm. This method works also with big genomes</option>
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81 </param>
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82 </when>
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83 </conditional>
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84 </macro>
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85
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86 <macro name="dbKeyActionsBwaMem">
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87 <expand macro="dbKeyActions">
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88 <option type="from_data_table" name="bwa_mem_indexes" column="1" offset="0">
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89 <filter type="param_value" column="0" value="#" compare="startswith" keep="False"/>
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90 <filter type="param_value" ref="reference_source.ref_file" column="0"/>
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91 </option>
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92 </expand>
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93 </macro>
0
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94
8
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95 <macro name="dbKeyActions">
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96 <actions>
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97 <conditional name="reference_source.reference_source_selector">
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98 <when value="cached">
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99 <action type="metadata" name="dbkey">
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100 <yield />
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101 </action>
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102 </when>
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103 <when value="history">
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104 <action type="metadata" name="dbkey">
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105 <option type="from_param" name="reference_source.ref_file" param_attribute="dbkey" />
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106 </action>
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107 </when>
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108 </conditional>
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109 </actions>
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110 </macro>
0
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111
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112 <token name="@ref_genomes@">
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113 **Indices: Selecting reference genomes for BWA**
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114
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115 The Galaxy wrapper for BWA allows you to select between precomputed and user-defined indices for reference genomes
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116 using the **Will you select a reference genome from your history or use a built-in index?** select box.
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117
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118 This select box has two options:
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119
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120 1. **Use a built-in genome index**
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121
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122 With this option (which is the default), Galaxy provides you with a dropdown select menu populated with genomes that have been pre-indexed with the bwa index utility and are ready to map sequenced reads against.
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123
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124 The collection of pre-indexed genomes is managed by the administrators of your Galaxy instance. If your genome of interest is missing and its impractical to use the second option below to work with it, consider contacting the support team for the Galaxy server you are working on to let them know that you would like to have an additional genome indexed.
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125
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126 2. **Use a genome from history and build index**
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127
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128 With this option, Galaxy provides you with a dropdown select menu populated with all FASTA formatted files listed in your current history. If you have uploaded your genome of interest into your history it will be shown there.
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129
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130 Selecting a genome from this dropdown will cause Galaxy to index it transparently first using the `bwa index` command, and then map against it with `bwa aln`.
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131 </token>
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132 <token name="@RG@">
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133 -----
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134
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135 .. class:: warningmark
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136
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137 **Read Groups are Important!**
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138
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139 One of the recommended best practices in NGS analysis is adding read group information to BAM files. You can do this directly in BWA interface using the
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140 **Specify read group information?** widget. If you are not familiar with read groups you shold know that this is effectively a way to tag reads with an additional ID.
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141 This allows you to combine BAM files from, for example, multiple BWA runs into a single dataset. This significantly simplifies downstream processing as
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142 instead of dealing with multiple datasets you only have to handle only one. This is possible because the read group information allows you to identify
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143 data from different experiments even if they are combined in one file. Many downstream analysis tools such as variant callers (e.g., FreeBayes or Naive Variant Caller
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144 present in Galaxy) are aware of read groups and will automatically generate calls for each individual sample even if they are combined within a single file.
0
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145
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146 **Description of read groups fields**
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147
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148 (from GATK FAQ webpage):
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149
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150 .. csv-table::
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151 :header-rows: 1
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152
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153 Tag,Importance,Definition,Meaning
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154 "ID","Required","Read group identifier. Each @RG line must have a unique ID. The value of ID is used in the RG tags of alignment records. Must be unique among all read groups in header section. Read group IDs may be modified when merging SAM files in order to handle collisions.","Ideally, this should be a globally unique identify across all sequencing data in the world, such as the Illumina flowcell + lane name and number. Will be referenced by each read with the RG:Z field, allowing tools to determine the read group information associated with each read, including the sample from which the read came. Also, a read group is effectively treated as a separate run of the NGS instrument in tools like base quality score recalibration (a GATK component) -- all reads within a read group are assumed to come from the same instrument run and to therefore share the same error model."
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155 "SM","Sample. Use pool name where a pool is being sequenced.","Required. As important as ID.","The name of the sample sequenced in this read group. GATK tools treat all read groups with the same SM value as containing sequencing data for the same sample. Therefore it's critical that the SM field be correctly specified, especially when using multi-sample tools like the Unified Genotyper (a GATK component)."
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156 "PL","Platform/technology used to produce the read. Valid values: ILLUMINA, SOLID, LS454, HELICOS and PACBIO.","Important. Not currently used in the GATK, but was in the past, and may return. The only way to known the sequencing technology used to generate the sequencing data","It's a good idea to use this field."
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157 "LB","DNA preparation library identify","Essential for MarkDuplicates","MarkDuplicates uses the LB field to determine which read groups might contain molecular duplicates, in case the same DNA library was sequenced on multiple lanes."
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158
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159
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160 **Example of Read Group usage**
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161
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162 Suppose we have a trio of samples: MOM, DAD, and KID. Each has two DNA libraries prepared, one with 400 bp inserts and another with 200 bp inserts. Each of these libraries is run on two lanes of an illumina hiseq, requiring 3 x 2 x 2 = 12 lanes of data. When the data come off the sequencer, we would create 12 BAM files, with the following @RG fields in the header::
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163
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164 Dad's data:
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165 @RG ID:FLOWCELL1.LANE1 PL:illumina LB:LIB-DAD-1 SM:DAD PI:200
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166 @RG ID:FLOWCELL1.LANE2 PL:illumina LB:LIB-DAD-1 SM:DAD PI:200
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167 @RG ID:FLOWCELL1.LANE3 PL:illumina LB:LIB-DAD-2 SM:DAD PI:400
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168 @RG ID:FLOWCELL1.LANE4 PL:illumina LB:LIB-DAD-2 SM:DAD PI:400
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169
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170 Mom's data:
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171 @RG ID:FLOWCELL1.LANE5 PL:illumina LB:LIB-MOM-1 SM:MOM PI:200
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172 @RG ID:FLOWCELL1.LANE6 PL:illumina LB:LIB-MOM-1 SM:MOM PI:200
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173 @RG ID:FLOWCELL1.LANE7 PL:illumina LB:LIB-MOM-2 SM:MOM PI:400
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174 @RG ID:FLOWCELL1.LANE8 PL:illumina LB:LIB-MOM-2 SM:MOM PI:400
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175
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176 Kid's data:
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177 @RG ID:FLOWCELL2.LANE1 PL:illumina LB:LIB-KID-1 SM:KID PI:200
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178 @RG ID:FLOWCELL2.LANE2 PL:illumina LB:LIB-KID-1 SM:KID PI:200
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179 @RG ID:FLOWCELL2.LANE3 PL:illumina LB:LIB-KID-2 SM:KID PI:400
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180 @RG ID:FLOWCELL2.LANE4 PL:illumina LB:LIB-KID-2 SM:KID PI:400
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181
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182 Note the hierarchical relationship between read groups (unique for each lane) to libraries (sequenced on two lanes) and samples (across four lanes, two lanes for each library).
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183 </token>
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184 <token name="@links@">
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185 .. _BWA: https://github.com/lh3/bwa
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186 .. _`BWA manual`: https://bio-bwa.sourceforge.net/bwa.shtml
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187 </token>
0
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188 </macros>