Mercurial > repos > dereeper > uniqprimer
diff uniqprimer-0.5.0/tmpz0OJOq/referenceprimers.ep3 @ 0:cdd8f911ad91 draft
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| author | dereeper |
|---|---|
| date | Fri, 07 Oct 2016 04:18:11 -0400 |
| parents | |
| children |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/uniqprimer-0.5.0/tmpz0OJOq/referenceprimers.ep3 Fri Oct 07 04:18:11 2016 -0400 @@ -0,0 +1,2552 @@ + +# EPRIMER3 RESULTS FOR seq_0 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 207 + FORWARD PRIMER 2 20 58.89 55.00 GATTAGCCCTGACCTTCAGC + + REVERSE PRIMER 189 20 60.21 55.00 CTGATTAGCCCTGACCCTCA + + + 2 PRODUCT SIZE: 205 + FORWARD PRIMER 2 20 58.89 55.00 GATTAGCCCTGACCTTCAGC + + REVERSE PRIMER 187 20 61.13 60.00 GATTAGCCCTGACCCTCAGC + + + + +# EPRIMER3 RESULTS FOR seq_1 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 202 + FORWARD PRIMER 211 20 60.49 50.00 CCAGTGATTTTCCGAAGTGG + + REVERSE PRIMER 393 20 59.85 55.00 TCTGACCGGCTGTATCACTG + + + 2 PRODUCT SIZE: 203 + FORWARD PRIMER 210 20 60.49 50.00 CCCAGTGATTTTCCGAAGTG + + REVERSE PRIMER 393 20 59.85 55.00 TCTGACCGGCTGTATCACTG + + + + +# EPRIMER3 RESULTS FOR seq_2 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 223 + FORWARD PRIMER 515 20 60.13 55.00 AACTGGACGATGCCTACCTG + + REVERSE PRIMER 718 20 59.71 55.00 GCACCGAGTCCATCACTGTA + + + 2 PRODUCT SIZE: 224 + FORWARD PRIMER 514 20 60.13 55.00 CAACTGGACGATGCCTACCT + + REVERSE PRIMER 718 20 59.71 55.00 GCACCGAGTCCATCACTGTA + + + + +# EPRIMER3 RESULTS FOR seq_3 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 230 + FORWARD PRIMER 13 20 60.07 50.00 CATCCTTCAGCCATGGAAGT + + REVERSE PRIMER 223 20 60.01 50.00 GTTGGTTGAATGTGGTGCTG + + + 2 PRODUCT SIZE: 216 + FORWARD PRIMER 151 20 60.43 55.00 CAGGTAGCGCCTTGCATACT + + REVERSE PRIMER 347 20 60.41 60.00 GAGCCGGAGTGTGAGGTCTA + + + + +# EPRIMER3 RESULTS FOR seq_4 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 12 20 60.30 50.00 ACGAACGGCTGTTTGTTCTC + + REVERSE PRIMER 193 19 59.72 57.90 GCAGCACTGGAGTTGAAGC + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 12 20 60.30 50.00 ACGAACGGCTGTTTGTTCTC + + REVERSE PRIMER 194 19 59.56 57.90 GGCAGCACTGGAGTTGAAG + + + + +# EPRIMER3 RESULTS FOR seq_5 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 220 + FORWARD PRIMER 5 20 60.30 55.00 CTTGCTGGAGGTCAACAGGT + + REVERSE PRIMER 205 20 60.26 55.00 GTGTTACCGGGCGTTGTATC + + + 2 PRODUCT SIZE: 221 + FORWARD PRIMER 18 20 60.55 50.00 AACAGGTACATCGCCTGCAT + + REVERSE PRIMER 219 20 60.11 50.00 GGAACCGAAGCCAAGTGTTA + + + + +# EPRIMER3 RESULTS FOR seq_6 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 90 20 59.67 55.00 ATAGCCTCCGGTTCTTCTCC + + REVERSE PRIMER 270 20 60.59 55.00 GGGTTTAGGTCGGGTGAAAC + + + 2 PRODUCT SIZE: 202 + FORWARD PRIMER 88 20 60.59 55.00 CCATAGCCTCCGGTTCTTCT + + REVERSE PRIMER 270 20 60.59 55.00 GGGTTTAGGTCGGGTGAAAC + + + + +# EPRIMER3 RESULTS FOR seq_7 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 211 + FORWARD PRIMER 66 20 59.99 55.00 TGAGCCAGAGGTTCAGGTCT + + REVERSE PRIMER 257 20 60.34 50.00 CAACACGCAAGGTTGTGAAG + + + 2 PRODUCT SIZE: 212 + FORWARD PRIMER 65 20 59.99 60.00 CTGAGCCAGAGGTTCAGGTC + + REVERSE PRIMER 257 20 60.34 50.00 CAACACGCAAGGTTGTGAAG + + + + +# EPRIMER3 RESULTS FOR seq_8 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 201 + FORWARD PRIMER 195 20 59.76 60.00 CTGTCGGGTAGTAGCGGAAC + + REVERSE PRIMER 376 20 60.33 50.00 TTTTCTCTGGCAGGAGGATG + + + 2 PRODUCT SIZE: 206 + FORWARD PRIMER 455 20 59.60 45.00 TCCATCATCAATCGTCTTCG + + REVERSE PRIMER 641 20 60.01 55.00 AGGACACGTGGAGGTTGTTC + + + + +# EPRIMER3 RESULTS FOR seq_9 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 202 + FORWARD PRIMER 155 20 60.05 50.00 GATGGACCGGGTAAAAACCT + + REVERSE PRIMER 337 20 59.59 50.00 TCGAAGTTGTCGTAGCTCCA + + + 2 PRODUCT SIZE: 203 + FORWARD PRIMER 154 20 60.05 50.00 AGATGGACCGGGTAAAAACC + + REVERSE PRIMER 337 20 59.59 50.00 TCGAAGTTGTCGTAGCTCCA + + + + +# EPRIMER3 RESULTS FOR seq_10 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 383 20 59.60 45.00 TCAATCAAGTGCGCTGCTAT + + REVERSE PRIMER 563 20 60.15 55.00 CGGCTACTACATTGGCAGGT + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 382 20 59.60 45.00 ATCAATCAAGTGCGCTGCTA + + REVERSE PRIMER 563 20 60.15 55.00 CGGCTACTACATTGGCAGGT + + + + +# EPRIMER3 RESULTS FOR seq_11 + +# Start Len Tm GC% Sequence + + + + + +# EPRIMER3 RESULTS FOR seq_12 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 202 + FORWARD PRIMER 862 20 60.43 55.00 GTACAGATCGCCGAGCACTT + + REVERSE PRIMER 1044 20 60.24 50.00 CCAATCCAGACTTGCGCTAT + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 862 20 60.43 55.00 GTACAGATCGCCGAGCACTT + + REVERSE PRIMER 1043 20 59.44 50.00 CAATCCAGACTTGCGCTATG + + + + +# EPRIMER3 RESULTS FOR seq_13 + +# Start Len Tm GC% Sequence + + + + + +# EPRIMER3 RESULTS FOR seq_14 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 220 + FORWARD PRIMER 32 20 60.01 50.00 AGAGTAGAAGCGGCAACCAA + + REVERSE PRIMER 232 20 59.77 55.00 CGAAGAAGCGTGAGTGTGAG + + + 2 PRODUCT SIZE: 221 + FORWARD PRIMER 31 20 60.01 50.00 AAGAGTAGAAGCGGCAACCA + + REVERSE PRIMER 232 20 59.77 55.00 CGAAGAAGCGTGAGTGTGAG + + + + +# EPRIMER3 RESULTS FOR seq_15 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 226 + FORWARD PRIMER 13 19 59.71 57.90 GCGAGTAAGCCAGCAGATG + + REVERSE PRIMER 219 20 60.81 45.00 TTCATGCATCTACGCAAACG + + + 2 PRODUCT SIZE: 227 + FORWARD PRIMER 12 19 60.52 57.90 GGCGAGTAAGCCAGCAGAT + + REVERSE PRIMER 219 20 60.81 45.00 TTCATGCATCTACGCAAACG + + + + +# EPRIMER3 RESULTS FOR seq_16 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 216 + FORWARD PRIMER 53 20 59.99 55.00 ACTGAAGTCCTCCCTCAGCA + + REVERSE PRIMER 251 18 59.85 55.56 GAAACCGGGATGTCGATG + + + 2 PRODUCT SIZE: 217 + FORWARD PRIMER 53 20 59.99 55.00 ACTGAAGTCCTCCCTCAGCA + + REVERSE PRIMER 252 18 59.85 50.00 TGAAACCGGGATGTCGAT + + + + +# EPRIMER3 RESULTS FOR seq_17 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 206 + FORWARD PRIMER 3 21 59.83 42.86 TCGATTCAAAGCCTTACCTGA + + REVERSE PRIMER 189 20 58.35 45.00 GCTGGATTTTTGTTGCAGTC + + + 2 PRODUCT SIZE: 207 + FORWARD PRIMER 3 21 59.83 42.86 TCGATTCAAAGCCTTACCTGA + + REVERSE PRIMER 189 21 59.37 42.86 AGCTGGATTTTTGTTGCAGTC + + + + +# EPRIMER3 RESULTS FOR seq_18 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 836 19 59.91 57.90 ATCTGTCCGGGTTGGACTC + + REVERSE PRIMER 1016 20 59.85 55.00 GGTCTTGAGCACTTCCGAAC + + + 2 PRODUCT SIZE: 209 + FORWARD PRIMER 1120 20 60.94 55.00 CAGATCATGTACCCGGTGCT + + REVERSE PRIMER 1309 20 59.98 50.00 GCATCGCTGATGAAGTCGTA + + + + +# EPRIMER3 RESULTS FOR seq_19 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 202 + FORWARD PRIMER 1286 20 59.21 55.00 CAGTAGCAACCCCTTCAGGT + + REVERSE PRIMER 1468 20 60.10 50.00 CGTTCGGGTTCTTAGTTCCA + + + 2 PRODUCT SIZE: 217 + FORWARD PRIMER 1373 20 60.16 50.00 AAAACGCACTTCCAGTGACC + + REVERSE PRIMER 1570 20 60.01 55.00 AGAGAGACGGACGCTTGGTA + + + + +# EPRIMER3 RESULTS FOR seq_20 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 554 20 59.93 50.00 GGACAGCCCTGTATGGAAAA + + REVERSE PRIMER 734 20 60.97 50.00 TGGAAGAAGCAGTTGCCAGT + + + 2 PRODUCT SIZE: 209 + FORWARD PRIMER 554 20 59.93 50.00 GGACAGCCCTGTATGGAAAA + + REVERSE PRIMER 743 20 60.68 55.00 GGAGCTGACTGGAAGAAGCA + + + + +# EPRIMER3 RESULTS FOR seq_21 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 205 + FORWARD PRIMER 360 20 60.16 55.00 AAGCAGGTCTGGAACACCAC + + REVERSE PRIMER 545 20 60.83 55.00 GGAGTGCGTTAAAGCAGGTG + + + 2 PRODUCT SIZE: 208 + FORWARD PRIMER 200 20 60.37 45.00 TTTTTCCTGCACGGTGGTAT + + REVERSE PRIMER 388 20 59.44 50.00 TCCGTACTGGCAGTTCTTCA + + + + +# EPRIMER3 RESULTS FOR seq_22 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 213 + FORWARD PRIMER 1197 20 59.97 50.00 GCTTTGACCTTGTGGTCCAT + + REVERSE PRIMER 1390 20 59.85 55.00 CCAGATCAGCGTCAGTGGTA + + + 2 PRODUCT SIZE: 205 + FORWARD PRIMER 1557 20 59.86 50.00 CAAATGAGACGTCTGCGGTA + + REVERSE PRIMER 1742 20 60.45 50.00 TGCCTTTGTAGCACTGTGGA + + + + +# EPRIMER3 RESULTS FOR seq_23 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 204 + FORWARD PRIMER 109 20 59.68 50.00 CGTCATCAAACCAGACCTGA + + REVERSE PRIMER 293 20 59.98 50.00 TGGACTAGAAGCGCCTTGAT + + + 2 PRODUCT SIZE: 213 + FORWARD PRIMER 178 20 59.90 50.00 AAACGCTAAGCCGGTACTGA + + REVERSE PRIMER 371 20 60.29 55.00 GTGATGATGTCGAGCGTGTC + + + + +# EPRIMER3 RESULTS FOR seq_24 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 203 + FORWARD PRIMER 20 20 59.22 50.00 CTGAAGGCAACGTTCTGCTA + + REVERSE PRIMER 204 19 59.88 52.63 GTCTCCCATTTGCCCTGAT + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 22 20 60.96 55.00 GAAGGCAACGTTCTGCTACG + + REVERSE PRIMER 204 19 59.88 52.63 GTCTCCCATTTGCCCTGAT + + + + +# EPRIMER3 RESULTS FOR seq_25 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 235 + FORWARD PRIMER 82 20 60.65 55.00 CGCAAGGTATCCAGTTCCTG + + REVERSE PRIMER 297 20 59.99 60.00 GGACCTCAGTGAAGGAGCAG + + + 2 PRODUCT SIZE: 239 + FORWARD PRIMER 87 20 59.31 55.00 GGTATCCAGTTCCTGCTGCT + + REVERSE PRIMER 306 20 60.32 60.00 AGCACTACCGGACCTCAGTG + + + + +# EPRIMER3 RESULTS FOR seq_26 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 202 + FORWARD PRIMER 173 20 60.24 50.00 CGCAGCTGGTATGGATTTCT + + REVERSE PRIMER 355 20 60.04 50.00 ATCACAGCGCACAGCATAAG + + + 2 PRODUCT SIZE: 205 + FORWARD PRIMER 409 20 60.17 55.00 CGGACATGTACGACGAGTTG + + REVERSE PRIMER 594 20 59.94 50.00 GACTCCCAAACCTGGAACAA + + + + +# EPRIMER3 RESULTS FOR seq_27 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 1945 20 60.29 50.00 GTCACGATCCCGAATCAATC + + REVERSE PRIMER 2125 20 60.00 55.00 GACAGTTCTTCGGCGTTCTC + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 6075 20 60.10 50.00 TGGCGACTATGTCGGATGTA + + REVERSE PRIMER 6255 20 60.24 50.00 GATGGTAGCGCGATGACTTT + + + + +# EPRIMER3 RESULTS FOR seq_28 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 205 + FORWARD PRIMER 350 20 60.25 50.00 TCAACGCCCTTCTTTACGTC + + REVERSE PRIMER 535 20 60.12 55.00 ACCTTGATGCTGGTGGAGTC + + + 2 PRODUCT SIZE: 209 + FORWARD PRIMER 321 20 59.97 50.00 CAATGTCAGCATGACCAACC + + REVERSE PRIMER 510 20 60.21 45.00 AGACCGCTTCGATTTTGATG + + + + +# EPRIMER3 RESULTS FOR seq_29 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 201 + FORWARD PRIMER 278 20 60.33 50.00 TTTTCTCTGGCAGGAGGATG + + REVERSE PRIMER 459 20 59.76 60.00 CTGTCGGGTAGTAGCGGAAC + + + 2 PRODUCT SIZE: 211 + FORWARD PRIMER 534 20 60.10 50.00 TATGCCGTGGATGTAGACGA + + REVERSE PRIMER 725 20 59.98 50.00 TGATCTGCTTCCACAACTCG + + + + +# EPRIMER3 RESULTS FOR seq_30 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 202 + FORWARD PRIMER 588 20 59.90 55.00 ACAGCGGCTACACTGGAGAT + + REVERSE PRIMER 770 20 59.48 50.00 AAGCTGGTATTGAGCCTTCG + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 556 20 60.59 55.00 GGGTTTAGGTCGGGTGAAAC + + REVERSE PRIMER 736 20 59.67 55.00 ATAGCCTCCGGTTCTTCTCC + + + + +# EPRIMER3 RESULTS FOR seq_31 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 205 + FORWARD PRIMER 592 20 60.25 50.00 TCAACGCCCTTCTTTACGTC + + REVERSE PRIMER 777 20 60.12 55.00 ACCTTGATGCTGGTGGAGTC + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 421 20 59.55 40.00 AAAATCCATCGGCGAAGTAA + + REVERSE PRIMER 602 20 60.16 50.00 CATGCTCTGCGACGTAAAGA + + + + +# EPRIMER3 RESULTS FOR seq_32 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 205 + FORWARD PRIMER 93 20 60.30 50.00 GGATTGGTTGATCGATACGC + + REVERSE PRIMER 278 20 60.11 55.00 TCCCTAGCCGAAGAGCACTA + + + 2 PRODUCT SIZE: 213 + FORWARD PRIMER 251 20 59.96 50.00 GCGAGAACTTTCATCCGAAG + + REVERSE PRIMER 444 20 59.88 40.00 CTTACGACGCAAAATGCAAA + + + + +# EPRIMER3 RESULTS FOR seq_33 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 225 + FORWARD PRIMER 117 20 60.67 55.00 GCACCGACATGAAGTTCCTC + + REVERSE PRIMER 322 20 60.39 55.00 TCAGGCAGTTCTTCCAGGTC + + + 2 PRODUCT SIZE: 216 + FORWARD PRIMER 87 20 59.49 55.00 GGGAGGAGGTCTTCTTCCAT + + REVERSE PRIMER 282 21 60.01 47.62 CGTACTTGTCCAATGGATGCT + + + + +# EPRIMER3 RESULTS FOR seq_34 + +# Start Len Tm GC% Sequence + + + + + +# EPRIMER3 RESULTS FOR seq_35 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 37 20 59.22 55.00 AAGCTACTCGGTGGATACGG + + REVERSE PRIMER 217 20 61.15 50.00 AGTTCATCTGCCAGCCCTTT + + + 2 PRODUCT SIZE: 202 + FORWARD PRIMER 19 20 60.37 45.00 GACGGTCAAAAAGAGCGAAA + + REVERSE PRIMER 202 19 59.38 52.63 CTTTGTGCTGGGAATGCTC + + + + +# EPRIMER3 RESULTS FOR seq_36 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 212 20 60.43 50.00 CCTTGACCAGTTGCAGCTTT + + REVERSE PRIMER 392 20 59.36 55.00 GTTCCTGATCCTGGACAACC + + + 2 PRODUCT SIZE: 209 + FORWARD PRIMER 24 20 59.55 45.00 TTGCAAAGGACACCACAATC + + REVERSE PRIMER 213 20 59.63 50.00 CAAAGCTGCAACTGGTCAAG + + + + +# EPRIMER3 RESULTS FOR seq_37 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 223 + FORWARD PRIMER 137 20 59.82 50.00 GCTACATGAAACCGGGATGT + + REVERSE PRIMER 340 20 59.99 55.00 ACTGAAGTCCTCCCTCAGCA + + + 2 PRODUCT SIZE: 222 + FORWARD PRIMER 137 20 59.82 50.00 GCTACATGAAACCGGGATGT + + REVERSE PRIMER 339 20 60.13 60.00 CTGAAGTCCTCCCTCAGCAG + + + + +# EPRIMER3 RESULTS FOR seq_38 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 201 + FORWARD PRIMER 52 20 59.99 45.00 CGTTGCGAATCAGAGTTGAA + + REVERSE PRIMER 233 20 59.78 40.00 GTTTCCGGTTCCATCAAAAA + + + 2 PRODUCT SIZE: 217 + FORWARD PRIMER 290 20 59.97 45.00 TAGCCGCTTTATTCGATGCT + + REVERSE PRIMER 487 20 59.95 45.00 GGTTTTCCTGACGTTTTCCA + + + + +# EPRIMER3 RESULTS FOR seq_39 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 206 + FORWARD PRIMER 579 20 59.79 50.00 TTGGTCTGATGCATCTCAGG + + REVERSE PRIMER 765 20 60.26 55.00 CCAGACGAGACCACGATTCT + + + 2 PRODUCT SIZE: 209 + FORWARD PRIMER 579 20 59.79 50.00 TTGGTCTGATGCATCTCAGG + + REVERSE PRIMER 768 20 60.12 55.00 GTTCCAGACGAGACCACGAT + + + + +# EPRIMER3 RESULTS FOR seq_40 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 228 + FORWARD PRIMER 200 20 60.28 50.00 TGGTCTGAAACACGACCTGA + + REVERSE PRIMER 408 20 59.97 45.00 ACGACACGGGATTTTCAAAG + + + 2 PRODUCT SIZE: 229 + FORWARD PRIMER 199 20 59.72 50.00 TTGGTCTGAAACACGACCTG + + REVERSE PRIMER 408 20 59.97 45.00 ACGACACGGGATTTTCAAAG + + + + +# EPRIMER3 RESULTS FOR seq_41 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 202 + FORWARD PRIMER 8 20 59.87 45.00 CGCGTCGTTGATGAGTAAAA + + REVERSE PRIMER 190 20 60.12 55.00 GCGGAGCTATGAAAGTGAGC + + + 2 PRODUCT SIZE: 207 + FORWARD PRIMER 3 20 59.97 50.00 TAGATCGCGTCGTTGATGAG + + REVERSE PRIMER 190 20 60.12 55.00 GCGGAGCTATGAAAGTGAGC + + + + +# EPRIMER3 RESULTS FOR seq_42 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 209 + FORWARD PRIMER 62 20 61.45 50.00 CGCCAACTGAACGAAAGCTA + + REVERSE PRIMER 251 20 60.00 45.00 AAGCGGGGTTTTTCGTAACT + + + 2 PRODUCT SIZE: 206 + FORWARD PRIMER 35 20 61.12 55.00 ACCACCGTTCTAGTGCATCG + + REVERSE PRIMER 221 20 59.42 45.00 TGCAAAGGGAGCTTGTATGA + + + + +# EPRIMER3 RESULTS FOR seq_43 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 207 + FORWARD PRIMER 4 20 58.89 55.00 GATTAGCCCTGACCTTCAGC + + REVERSE PRIMER 191 20 60.21 55.00 CTGATTAGCCCTGACCCTCA + + + 2 PRODUCT SIZE: 210 + FORWARD PRIMER 1 20 58.74 55.00 CCTGATTAGCCCTGACCTTC + + REVERSE PRIMER 191 20 60.21 55.00 CTGATTAGCCCTGACCCTCA + + + + +# EPRIMER3 RESULTS FOR seq_44 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 201 + FORWARD PRIMER 4 19 59.45 57.90 GATTAGCCCCATCCCTCAG + + REVERSE PRIMER 187 18 57.88 55.56 ATCAGCGGGACCTACCAT + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 5 19 61.33 57.90 ATTAGCCCCATCCCTCAGC + + REVERSE PRIMER 187 18 57.88 55.56 ATCAGCGGGACCTACCAT + + + + +# EPRIMER3 RESULTS FOR seq_45 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 228 + FORWARD PRIMER 45 20 59.23 60.00 CCGGCACTGGTAGTAGACCT + + REVERSE PRIMER 253 20 60.66 55.00 GACAATGACCCCAGCTCAAG + + + 2 PRODUCT SIZE: 212 + FORWARD PRIMER 17 19 61.13 52.63 CACGCAAGGTGGTTTGATG + + REVERSE PRIMER 209 20 59.60 50.00 CAATCTCGTGCAGTTTCAGC + + + + +# EPRIMER3 RESULTS FOR seq_46 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 202 + FORWARD PRIMER 977 20 59.35 55.00 ACAGGACAGAGCCTTTACCG + + REVERSE PRIMER 1159 20 60.13 50.00 TTACGCCACCCTTACTTTGC + + + 2 PRODUCT SIZE: 204 + FORWARD PRIMER 366 20 60.62 50.00 ACGTTGGATGCGATCCTAGA + + REVERSE PRIMER 550 20 59.94 45.00 GGCGAATTGTGAGGTGAAAT + + + + +# EPRIMER3 RESULTS FOR seq_47 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 204 + FORWARD PRIMER 382 20 59.81 45.00 ATAAGCGCATCCTTCCTCAA + + REVERSE PRIMER 566 20 60.39 45.00 TGCCGTCTTCTTCATGTTCA + + + 2 PRODUCT SIZE: 207 + FORWARD PRIMER 465 20 60.15 45.00 TGTCCGCAACCTGTTTACAA + + REVERSE PRIMER 652 20 60.30 50.00 GACGTTGTTGAACGCCTCTT + + + + +# EPRIMER3 RESULTS FOR seq_48 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 1509 20 59.88 55.00 CGGTAGGCCCACTATGTTGT + + REVERSE PRIMER 1689 20 60.11 45.00 CTTGTTCTGCCCGTTTCAAT + + + 2 PRODUCT SIZE: 202 + FORWARD PRIMER 1244 20 59.74 55.00 CGAGACGGTGTAGTTGTGGA + + REVERSE PRIMER 1426 20 59.97 45.00 CCTGCAAACTGGGTTTCAAT + + + + +# EPRIMER3 RESULTS FOR seq_49 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 211 + FORWARD PRIMER 48 20 60.19 50.00 GAAACCCCTTGGTGCCTATT + + REVERSE PRIMER 240 19 60.10 42.10 AACGCGTTGTTTCAATCCA + + + 2 PRODUCT SIZE: 202 + FORWARD PRIMER 57 20 59.18 45.00 TGGTGCCTATTGTGCCTAAA + + REVERSE PRIMER 240 19 60.10 42.10 AACGCGTTGTTTCAATCCA + + + + +# EPRIMER3 RESULTS FOR seq_50 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 212 + FORWARD PRIMER 197 20 59.54 55.00 CTATAGCGGCCGTAGTTGCT + + REVERSE PRIMER 389 20 59.48 60.00 GGCTCTTCGAGCTAGTCAGC + + + 2 PRODUCT SIZE: 214 + FORWARD PRIMER 197 20 59.54 55.00 CTATAGCGGCCGTAGTTGCT + + REVERSE PRIMER 390 21 59.91 52.38 TTGGCTCTTCGAGCTAGTCAG + + + + +# EPRIMER3 RESULTS FOR seq_51 + +# Start Len Tm GC% Sequence + + + + + +# EPRIMER3 RESULTS FOR seq_52 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 206 + FORWARD PRIMER 127 20 59.03 45.00 GCACTCAAACCCCTGATTTT + + REVERSE PRIMER 313 20 60.12 50.00 GGGTGATCAAGCGTCAGTTT + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 45 20 58.53 50.00 CATTAGCTCACCGTCTTTCG + + REVERSE PRIMER 226 20 59.98 50.00 TGTCGAATCTGTGGCTGAAG + + + + +# EPRIMER3 RESULTS FOR seq_53 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 203 + FORWARD PRIMER 18 18 59.21 55.56 CTGTGCATTCGACGTGGT + + REVERSE PRIMER 202 19 60.41 57.90 GTCGCGTAAAGGGCAGAGT + + + 2 PRODUCT SIZE: 202 + FORWARD PRIMER 19 18 61.40 55.56 TGTGCATTCGACGTGGTG + + REVERSE PRIMER 202 19 60.41 57.90 GTCGCGTAAAGGGCAGAGT + + + + +# EPRIMER3 RESULTS FOR seq_54 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 18 20 59.84 55.00 GCCTCGACTTTGGATAGTGC + + REVERSE PRIMER 198 20 60.23 55.00 CTCTGAACAACTCCCCCTGA + + + 2 PRODUCT SIZE: 203 + FORWARD PRIMER 15 20 59.98 55.00 GCTGCCTCGACTTTGGATAG + + REVERSE PRIMER 198 20 60.23 55.00 CTCTGAACAACTCCCCCTGA + + + + +# EPRIMER3 RESULTS FOR seq_55 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 215 + FORWARD PRIMER 61 20 60.10 50.00 GCATCCACACGCCTATTCTT + + REVERSE PRIMER 256 20 59.98 55.00 CTTGCTCTTCAAGGGGTCTG + + + 2 PRODUCT SIZE: 217 + FORWARD PRIMER 59 20 60.10 50.00 AAGCATCCACACGCCTATTC + + REVERSE PRIMER 256 20 59.98 55.00 CTTGCTCTTCAAGGGGTCTG + + + + +# EPRIMER3 RESULTS FOR seq_56 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 2453 20 59.94 40.00 AACGCATCGGCAAAATAATC + + REVERSE PRIMER 2633 20 60.23 55.00 GTCTAACATGGGGGTTGTCG + + + 2 PRODUCT SIZE: 205 + FORWARD PRIMER 3301 20 60.01 55.00 GCGACCTGCTTTTAGACCTG + + REVERSE PRIMER 3486 20 60.03 50.00 GCAAAGTCCACTTGCACAGA + + + + +# EPRIMER3 RESULTS FOR seq_57 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 950 20 59.95 55.00 ATCGTCCTGACACCGGATAG + + REVERSE PRIMER 1130 20 59.62 45.00 CTGAACAACGCACCACAAAT + + + 2 PRODUCT SIZE: 206 + FORWARD PRIMER 595 20 59.79 50.00 TTGGTCTGATGCATCTCAGG + + REVERSE PRIMER 781 20 60.26 55.00 CCAGACGAGACCACGATTCT + + + + +# EPRIMER3 RESULTS FOR seq_58 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 216 + FORWARD PRIMER 279 20 60.29 55.00 CGCTAACTGCCGGACTGTAT + + REVERSE PRIMER 475 20 59.97 45.00 AAAGCAATTGGCGGTATGAC + + + 2 PRODUCT SIZE: 218 + FORWARD PRIMER 279 20 60.29 55.00 CGCTAACTGCCGGACTGTAT + + REVERSE PRIMER 477 20 60.10 45.00 AGAAAGCAATTGGCGGTATG + + + + +# EPRIMER3 RESULTS FOR seq_59 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 448 20 59.27 45.00 ATCGAACATGGCCTGGTAAT + + REVERSE PRIMER 628 20 58.90 45.00 TGGAAACCTTCAAGTTGCTG + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 448 20 59.27 45.00 ATCGAACATGGCCTGGTAAT + + REVERSE PRIMER 628 21 59.77 47.62 GTGGAAACCTTCAAGTTGCTG + + + + +# EPRIMER3 RESULTS FOR seq_60 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 226 + FORWARD PRIMER 6 20 59.33 60.00 GTAGCACTCCTCGGTCCACT + + REVERSE PRIMER 213 19 60.34 52.63 CGCTAATCCGCATCATCTG + + + 2 PRODUCT SIZE: 225 + FORWARD PRIMER 6 20 59.33 60.00 GTAGCACTCCTCGGTCCACT + + REVERSE PRIMER 212 19 59.35 52.63 GCTAATCCGCATCATCTGC + + + + +# EPRIMER3 RESULTS FOR seq_61 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 183 20 60.25 45.00 ACGACGAATTGGAAAAGCTG + + REVERSE PRIMER 363 20 60.10 50.00 CTGGCGTAGACGGAAATGAT + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 351 20 59.89 50.00 TGGTGGAGGGCTATCATTTC + + REVERSE PRIMER 532 20 59.67 45.00 AAATAAGGGCATGCAGAGGA + + + + +# EPRIMER3 RESULTS FOR seq_62 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 202 + FORWARD PRIMER 1087 20 59.82 50.00 TCTACGCCGATGAAGTGATG + + REVERSE PRIMER 1269 20 60.14 55.00 ACCAGGCACAGCTGGTAATC + + + 2 PRODUCT SIZE: 203 + FORWARD PRIMER 1082 20 59.83 55.00 GGCTATCTACGCCGATGAAG + + REVERSE PRIMER 1265 20 60.14 55.00 GGCACAGCTGGTAATCCAGT + + + + +# EPRIMER3 RESULTS FOR seq_63 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 206 + FORWARD PRIMER 71 20 60.70 50.00 TGGCTCGGCCATCTCTATAA + + REVERSE PRIMER 257 20 60.07 50.00 ACTTCCATGGCTGAAGGATG + + + 2 PRODUCT SIZE: 223 + FORWARD PRIMER 39 20 60.07 50.00 CCAACATCCTTCCAGCCTAA + + REVERSE PRIMER 242 20 60.12 50.00 GGATGGGGCTAATCAGGAAT + + + + +# EPRIMER3 RESULTS FOR seq_64 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 215 + FORWARD PRIMER 5 20 61.91 55.00 CGATCCATGAACTCGCTCAG + + REVERSE PRIMER 200 20 58.89 40.00 CGCAAGTCTCATTGGAAAAA + + + 2 PRODUCT SIZE: 216 + FORWARD PRIMER 5 20 61.91 55.00 CGATCCATGAACTCGCTCAG + + REVERSE PRIMER 201 20 61.14 45.00 CCGCAAGTCTCATTGGAAAA + + + + +# EPRIMER3 RESULTS FOR seq_65 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 205 + FORWARD PRIMER 738 20 59.85 45.00 TCCAAAGCGTCTGGCTTTAT + + REVERSE PRIMER 923 20 60.10 55.00 TTGGCCGTAGAGGTATCGTC + + + 2 PRODUCT SIZE: 208 + FORWARD PRIMER 100 20 59.96 45.00 AAAGGCCAGCATCAAGAAGA + + REVERSE PRIMER 288 20 60.22 45.00 TTGGCCTTTCAAAGAAGGTG + + + + +# EPRIMER3 RESULTS FOR seq_66 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 203 + FORWARD PRIMER 861 20 59.89 50.00 ATGGTCGATGACGAGGATTC + + REVERSE PRIMER 1044 20 60.38 50.00 TCGGTTCTGTTTCAGGCTTC + + + 2 PRODUCT SIZE: 204 + FORWARD PRIMER 860 20 59.89 50.00 GATGGTCGATGACGAGGATT + + REVERSE PRIMER 1044 20 60.38 50.00 TCGGTTCTGTTTCAGGCTTC + + + + +# EPRIMER3 RESULTS FOR seq_67 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 210 + FORWARD PRIMER 5 20 61.25 50.00 GTGCACTTCCGACAGCAAAT + + REVERSE PRIMER 195 20 60.51 50.00 CCGAGAAGTTGAGCAAGGAA + + + 2 PRODUCT SIZE: 213 + FORWARD PRIMER 2 19 59.85 52.63 ATTGTGCACTTCCGACAGC + + REVERSE PRIMER 195 20 60.51 50.00 CCGAGAAGTTGAGCAAGGAA + + + + +# EPRIMER3 RESULTS FOR seq_68 + +# Start Len Tm GC% Sequence + + + + + +# EPRIMER3 RESULTS FOR seq_69 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 204 + FORWARD PRIMER 573 20 60.44 45.00 ATGGCCAATTCCAGAGCATA + + REVERSE PRIMER 757 20 60.00 50.00 CCAACACAGTTTCGGATGTG + + + 2 PRODUCT SIZE: 212 + FORWARD PRIMER 284 20 60.04 50.00 AAGGCAAGTACGCAGAGCAT + + REVERSE PRIMER 476 20 59.55 55.00 CCCAAGGACAGGATAGTTGC + + + + +# EPRIMER3 RESULTS FOR seq_70 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 4307 20 60.29 55.00 CCAGCGTACTGATGTCGATG + + REVERSE PRIMER 4487 20 60.03 45.00 CTTCGACAAAACGCACTCAA + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 2300 20 59.89 55.00 CAGCTGATGCTCAAGCAGAC + + REVERSE PRIMER 2480 20 60.34 55.00 GAATAGGGCAGGGTGATGTG + + + + +# EPRIMER3 RESULTS FOR seq_71 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 201 + FORWARD PRIMER 1083 20 60.09 55.00 CAACCACCTACCCATCCAAC + + REVERSE PRIMER 1265 19 59.97 52.63 ATGCTCATGCCTTGCAGAC + + + 2 PRODUCT SIZE: 203 + FORWARD PRIMER 1081 20 60.05 50.00 ATCAACCACCTACCCATCCA + + REVERSE PRIMER 1265 19 59.97 52.63 ATGCTCATGCCTTGCAGAC + + + + +# EPRIMER3 RESULTS FOR seq_72 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 202 + FORWARD PRIMER 861 20 59.02 55.00 GGCACTACCTGGGTGAAGAT + + REVERSE PRIMER 1043 20 59.92 50.00 GCAGGATGTAGGGCATGATT + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 590 19 59.94 52.63 GTATTACTCCGGCGCCATT + + REVERSE PRIMER 771 20 60.08 45.00 ATGAACTTGCCTTCCATTGC + + + + +# EPRIMER3 RESULTS FOR seq_73 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 201 + FORWARD PRIMER 378 20 60.63 55.00 GAGCCCATCAGTTCGATGAC + + REVERSE PRIMER 559 20 60.19 50.00 TGGCTGAAGGATTTCCAGAC + + + 2 PRODUCT SIZE: 202 + FORWARD PRIMER 378 20 60.63 55.00 GAGCCCATCAGTTCGATGAC + + REVERSE PRIMER 560 20 60.19 50.00 GTGGCTGAAGGATTTCCAGA + + + + +# EPRIMER3 RESULTS FOR seq_74 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 222 + FORWARD PRIMER 277 20 59.93 45.00 ACGAATGGATCGAAAACTGG + + REVERSE PRIMER 479 20 59.86 55.00 CCGAACTGAGTGTCCACAGA + + + 2 PRODUCT SIZE: 226 + FORWARD PRIMER 273 20 60.04 45.00 GGCTACGAATGGATCGAAAA + + REVERSE PRIMER 479 20 59.86 55.00 CCGAACTGAGTGTCCACAGA + + + + +# EPRIMER3 RESULTS FOR seq_75 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 4903 20 60.41 55.00 CTCGCTGGAAGTGGATGAGT + + REVERSE PRIMER 5083 20 60.08 50.00 GAGATGGCGATGTTGAAGGT + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 5164 20 59.56 50.00 ATCGTGCTGTTGATGACCAC + + REVERSE PRIMER 5345 20 59.53 50.00 GGTGATGACCACGAAGTTGA + + + + +# EPRIMER3 RESULTS FOR seq_76 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 141 20 61.37 55.00 CAACCTGGATGTGGTCTTGG + + REVERSE PRIMER 321 20 60.69 55.00 GTTCCAGTTCGGAGAACACG + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 140 20 61.37 55.00 CCAACCTGGATGTGGTCTTG + + REVERSE PRIMER 321 20 60.69 55.00 GTTCCAGTTCGGAGAACACG + + + + +# EPRIMER3 RESULTS FOR seq_77 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 209 20 61.15 60.00 GCGACGTCTCGCTGAACTAC + + REVERSE PRIMER 389 20 60.26 45.00 GATGCGTTCGACGAAAAACT + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 209 19 60.30 57.90 GCGACGTCTCGCTGAACTA + + REVERSE PRIMER 389 20 60.26 45.00 GATGCGTTCGACGAAAAACT + + + + +# EPRIMER3 RESULTS FOR seq_78 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 203 + FORWARD PRIMER 6514 20 59.98 50.00 CAGTCCTGTGATTTCGCTCA + + REVERSE PRIMER 6697 20 60.00 55.00 GTGAGCGGTGGAGTAATGGT + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 3409 20 60.22 50.00 CTCCAGCGATCATTGAGGTT + + REVERSE PRIMER 3589 20 59.98 45.00 AATTCCAGCACACCGAAAAC + + + + +# EPRIMER3 RESULTS FOR seq_79 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 221 + FORWARD PRIMER 183 20 60.13 55.00 TCAGGAGCTGAAGTCGGAGT + + REVERSE PRIMER 384 20 59.79 50.00 AATCGAGTTGGGATGGTGAC + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 234 19 59.96 52.63 GCGTGGTTTTCACCATCAC + + REVERSE PRIMER 415 20 59.61 55.00 TAAGCCTAGCCAGTCCGAAG + + + + +# EPRIMER3 RESULTS FOR seq_80 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 203 + FORWARD PRIMER 2488 20 60.09 60.00 CGTGGTCGTACGTACCTGTG + + REVERSE PRIMER 2671 20 59.99 55.00 AGGTCAGCCATTACCCACTG + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 414 20 59.93 50.00 CAGGACCGGAACCTTCATAA + + REVERSE PRIMER 594 20 60.21 50.00 CTATGGCTTGACCCTCGAAA + + + + +# EPRIMER3 RESULTS FOR seq_81 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 204 + FORWARD PRIMER 448 20 60.07 45.00 GAGTTGTCGCCGGAAAAATA + + REVERSE PRIMER 632 20 59.46 45.00 GCTATTGGCAAAGCTTCGAT + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 519 20 60.16 50.00 GCGATCGCCATCCTAGATAA + + REVERSE PRIMER 700 20 60.62 55.00 CCATAGGTGATGCCTTCTCG + + + + +# EPRIMER3 RESULTS FOR seq_82 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 207 + FORWARD PRIMER 45 20 59.79 55.00 ACGACGCAGTCCCTGTTAGT + + REVERSE PRIMER 232 20 59.70 50.00 GCGGAATTAGCGGTATCTTG + + + 2 PRODUCT SIZE: 203 + FORWARD PRIMER 305 20 59.83 50.00 GCAGGATTGTCATCAGCGTA + + REVERSE PRIMER 488 20 60.55 50.00 TATGATCCGTATGGGGCAAC + + + + +# EPRIMER3 RESULTS FOR seq_83 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 203 + FORWARD PRIMER 1191 20 60.09 60.00 CGTGGTCGTACGTACCTGTG + + REVERSE PRIMER 1374 20 59.99 55.00 AGGTCAGCCATTACCCACTG + + + 2 PRODUCT SIZE: 204 + FORWARD PRIMER 1191 20 60.09 60.00 CGTGGTCGTACGTACCTGTG + + REVERSE PRIMER 1375 20 59.99 55.00 CAGGTCAGCCATTACCCACT + + + + +# EPRIMER3 RESULTS FOR seq_84 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 216 + FORWARD PRIMER 83 20 60.26 55.00 ATACTGACTGGCCGCATAGC + + REVERSE PRIMER 279 20 60.02 55.00 ACATTGGCTACCGGTACTGC + + + 2 PRODUCT SIZE: 207 + FORWARD PRIMER 22 20 59.71 50.00 TATAAGTGCCTGCCATCGTG + + REVERSE PRIMER 209 20 60.46 50.00 CGGGCCAAAATGTCTATCTG + + + + +# EPRIMER3 RESULTS FOR seq_85 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 216 + FORWARD PRIMER 42 20 60.26 55.00 ATACTGACTGGCCGCATAGC + + REVERSE PRIMER 238 20 60.02 55.00 ACATTGGCTACCGGTACTGC + + + 2 PRODUCT SIZE: 214 + FORWARD PRIMER 42 20 60.26 55.00 ATACTGACTGGCCGCATAGC + + REVERSE PRIMER 236 20 60.15 55.00 ATTGGCTACCGGTACTGCTG + + + + +# EPRIMER3 RESULTS FOR seq_86 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 5881 20 59.91 45.00 TTTTGGCTGTGCTTTACACG + + REVERSE PRIMER 6061 20 59.98 55.00 CGACATCACCCACTACATCG + + + 2 PRODUCT SIZE: 203 + FORWARD PRIMER 2699 20 60.03 60.00 CCGGGGTACTCAGTGTCAGT + + REVERSE PRIMER 2882 20 59.86 50.00 CAGTTCGTCCAATACGCAGA + + + + +# EPRIMER3 RESULTS FOR seq_87 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 207 + FORWARD PRIMER 87 20 59.87 55.00 CTCAGCTGGAGGGTAAAACG + + REVERSE PRIMER 274 20 59.55 50.00 TGAGCCTGACTGAGGCAATA + + + 2 PRODUCT SIZE: 208 + FORWARD PRIMER 87 20 59.87 55.00 CTCAGCTGGAGGGTAAAACG + + REVERSE PRIMER 275 20 60.56 55.00 CTGAGCCTGACTGAGGCAAT + + + + +# EPRIMER3 RESULTS FOR seq_88 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 211 + FORWARD PRIMER 42 20 60.43 60.00 CCCCTACACCCCTACAATCC + + REVERSE PRIMER 233 20 60.01 55.00 AGTAATCGTGGTGCCGGTAG + + + 2 PRODUCT SIZE: 210 + FORWARD PRIMER 42 20 60.43 60.00 CCCCTACACCCCTACAATCC + + REVERSE PRIMER 232 20 59.88 55.00 GTAATCGTGGTGCCGGTAGT + + + + +# EPRIMER3 RESULTS FOR seq_89 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 205 + FORWARD PRIMER 137 20 60.01 50.00 ACAGCCAAAAGTGGGTTCAC + + REVERSE PRIMER 322 20 60.68 55.00 ACATGGTCGCCTCCAGACTA + + + 2 PRODUCT SIZE: 206 + FORWARD PRIMER 137 20 60.01 50.00 ACAGCCAAAAGTGGGTTCAC + + REVERSE PRIMER 323 20 60.68 55.00 TACATGGTCGCCTCCAGACT + + + + +# EPRIMER3 RESULTS FOR seq_90 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 3152 20 60.14 55.00 ACATCGTCGCCCTCTACAAC + + REVERSE PRIMER 3332 20 59.33 50.00 GAGTCGTTGGTGGCTTTTCT + + + 2 PRODUCT SIZE: 207 + FORWARD PRIMER 2844 20 59.87 55.00 CTCAGCTGGAGGGTAAAACG + + REVERSE PRIMER 3031 20 59.55 50.00 TGAGCCTGACTGAGGCAATA + + + + +# EPRIMER3 RESULTS FOR seq_91 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 207 + FORWARD PRIMER 66 20 60.06 50.00 GCGGGATGCACCTAAAGATA + + REVERSE PRIMER 254 19 60.68 57.90 ACTGGCGGAGAAGCTCAAG + + + 2 PRODUCT SIZE: 208 + FORWARD PRIMER 66 20 60.06 50.00 GCGGGATGCACCTAAAGATA + + REVERSE PRIMER 254 20 61.98 55.00 AACTGGCGGAGAAGCTCAAG + + + + +# EPRIMER3 RESULTS FOR seq_92 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 226 + FORWARD PRIMER 37 20 60.00 55.00 ACCGTGATTACCCTGCTCAC + + REVERSE PRIMER 243 20 59.81 50.00 ATACCACCGAATAGCGGATG + + + 2 PRODUCT SIZE: 231 + FORWARD PRIMER 37 20 60.00 55.00 ACCGTGATTACCCTGCTCAC + + REVERSE PRIMER 248 20 60.21 55.00 CCACCATACCACCGAATAGC + + + + +# EPRIMER3 RESULTS FOR seq_93 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 656 20 61.46 60.00 CTCGGGACGTTCCTACTGGT + + REVERSE PRIMER 836 20 60.00 45.00 ACGTAACGCACCGAAAAATC + + + 2 PRODUCT SIZE: 207 + FORWARD PRIMER 179 20 60.99 45.00 ATGGTCGAAAAAGGCTCGTT + + REVERSE PRIMER 366 20 60.13 50.00 ATAGGTTTCGTCGTGCTTGG + + + + +# EPRIMER3 RESULTS FOR seq_94 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 118 19 60.62 57.90 GAAGGTGGCGACCTCAATC + + REVERSE PRIMER 300 18 60.66 55.56 ATGCCAACCGTGAGCATC + + + 2 PRODUCT SIZE: 245 + FORWARD PRIMER 67 20 59.29 40.00 TTTTTGCTGTTCACCTCCAA + + REVERSE PRIMER 294 18 60.87 61.11 ACCGTGAGCATCGACCAG + + + + +# EPRIMER3 RESULTS FOR seq_95 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 204 + FORWARD PRIMER 21 21 59.99 47.62 TGGACCTTGTTACTGGCAATC + + REVERSE PRIMER 206 19 59.49 52.63 ATCATCCACCAGCGACAAC + + + 2 PRODUCT SIZE: 205 + FORWARD PRIMER 21 21 59.99 47.62 TGGACCTTGTTACTGGCAATC + + REVERSE PRIMER 206 20 61.51 55.00 GATCATCCACCAGCGACAAC + + + + +# EPRIMER3 RESULTS FOR seq_96 + +# Start Len Tm GC% Sequence + + + + + +# EPRIMER3 RESULTS FOR seq_97 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 201 + FORWARD PRIMER 437 20 59.70 45.00 ATGTCGCGGAGAAACTTCAT + + REVERSE PRIMER 618 20 59.71 50.00 CCTGTGTGGGACGGTTTTAT + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 266 21 60.33 52.38 ACCGACAGCTCGACATACTTG + + REVERSE PRIMER 446 20 60.12 50.00 GGCACCGACATGAAGTTTCT + + + + +# EPRIMER3 RESULTS FOR seq_98 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 205 + FORWARD PRIMER 72 20 61.04 50.00 CCGGAAAAATCGCTTAGGAG + + REVERSE PRIMER 257 20 59.56 55.00 GCGGATCTGGGTAGTGAAAC + + + 2 PRODUCT SIZE: 204 + FORWARD PRIMER 72 20 61.04 50.00 CCGGAAAAATCGCTTAGGAG + + REVERSE PRIMER 256 20 60.51 55.00 CGGATCTGGGTAGTGAAACG + + + + +# EPRIMER3 RESULTS FOR seq_99 + +# Start Len Tm GC% Sequence + + + + + +# EPRIMER3 RESULTS FOR seq_100 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 214 + FORWARD PRIMER 26 18 60.65 55.56 GCGTCGCTTGATCGAAAG + + REVERSE PRIMER 220 20 59.87 50.00 GCACGGTGAGTTTGCTCATA + + + 2 PRODUCT SIZE: 220 + FORWARD PRIMER 20 18 60.82 55.56 GATGGTGCGTCGCTTGAT + + REVERSE PRIMER 220 20 59.87 50.00 GCACGGTGAGTTTGCTCATA + + + + +# EPRIMER3 RESULTS FOR seq_101 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 201 + FORWARD PRIMER 5 20 60.07 50.00 AAGTCATGCCAATCCTGGAG + + REVERSE PRIMER 187 19 61.35 52.63 ATCACCGCCATCCACTCAT + + + 2 PRODUCT SIZE: 204 + FORWARD PRIMER 2 20 60.07 50.00 AGGAAGTCATGCCAATCCTG + + REVERSE PRIMER 187 19 61.35 52.63 ATCACCGCCATCCACTCAT + + + + +# EPRIMER3 RESULTS FOR seq_102 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 203 + FORWARD PRIMER 55 19 61.88 57.90 GCAATAGCGAAGCGGTGAC + + REVERSE PRIMER 238 20 60.35 45.00 CAATTCGCTTTCCAGCAGAT + + + 2 PRODUCT SIZE: 205 + FORWARD PRIMER 55 19 61.88 57.90 GCAATAGCGAAGCGGTGAC + + REVERSE PRIMER 240 20 60.39 45.00 AACAATTCGCTTTCCAGCAG + + + + +# EPRIMER3 RESULTS FOR seq_103 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 206 + FORWARD PRIMER 129 20 60.10 55.00 GGAGATCTTTGAGCGTGAGC + + REVERSE PRIMER 315 20 60.47 50.00 CGATAGCACCGGTTTGAATC + + + 2 PRODUCT SIZE: 202 + FORWARD PRIMER 99 20 60.10 50.00 GCGGATGCACTCCAAGTATT + + REVERSE PRIMER 281 20 60.78 50.00 GAGGCAGACGTTTTCGAATG + + + + +# EPRIMER3 RESULTS FOR seq_104 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 172 20 59.46 45.00 GAGCAGTTTTCGGCAGATTT + + REVERSE PRIMER 352 20 60.25 50.00 TCTTCGTTTACCTCGGCAAC + + + 2 PRODUCT SIZE: 202 + FORWARD PRIMER 133 20 59.09 50.00 CTGTTCGTCAACATCGATCC + + REVERSE PRIMER 315 20 59.96 55.00 CTAAGGTCTTGGGGTGGACA + + + + +# EPRIMER3 RESULTS FOR seq_105 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 209 + FORWARD PRIMER 91 19 60.30 47.37 AATCCATGAATCGGCTGGT + + REVERSE PRIMER 280 20 60.48 55.00 GGATTCAGTCCATCCAGCAC + + + 2 PRODUCT SIZE: 212 + FORWARD PRIMER 91 19 60.30 47.37 AATCCATGAATCGGCTGGT + + REVERSE PRIMER 283 20 60.47 55.00 GTCGGATTCAGTCCATCCAG + + + + +# EPRIMER3 RESULTS FOR seq_106 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 203 + FORWARD PRIMER 39 20 59.99 55.00 CTACGGGACCTGACGTTCAT + + REVERSE PRIMER 222 20 61.13 55.00 ACATGGTGCAGTTCGGCTAC + + + 2 PRODUCT SIZE: 202 + FORWARD PRIMER 39 20 59.99 55.00 CTACGGGACCTGACGTTCAT + + REVERSE PRIMER 222 19 60.28 57.90 CATGGTGCAGTTCGGCTAC + + + + +# EPRIMER3 RESULTS FOR seq_107 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 234 + FORWARD PRIMER 49 20 60.14 60.00 GCTGGAGCAGTCAGGAGTTC + + REVERSE PRIMER 263 20 59.40 45.00 TCTGTGGGCCATAAATGTTG + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 49 20 60.14 60.00 GCTGGAGCAGTCAGGAGTTC + + REVERSE PRIMER 230 20 62.27 50.00 GCGGTTGAAGCAGAAAAAGC + + + + +# EPRIMER3 RESULTS FOR seq_108 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 201 + FORWARD PRIMER 181 20 60.62 55.00 TATACGAGCCTGCCCAGGTA + + REVERSE PRIMER 362 20 60.05 45.00 TATCGGCGTATTCCGAAAAG + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 182 20 60.87 55.00 ATACGAGCCTGCCCAGGTAT + + REVERSE PRIMER 362 20 60.05 45.00 TATCGGCGTATTCCGAAAAG + + + + +# EPRIMER3 RESULTS FOR seq_109 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 201 + FORWARD PRIMER 58 20 59.89 50.00 TGGTGGAGGGCTATCATTTC + + REVERSE PRIMER 239 20 59.67 45.00 AAATAAGGGCATGCAGAGGA + + + 2 PRODUCT SIZE: 205 + FORWARD PRIMER 51 20 59.93 50.00 GACAAATTGGTGGAGGGCTA + + REVERSE PRIMER 236 20 60.36 55.00 TAAGGGCATGCAGAGGAGTC + + + + +# EPRIMER3 RESULTS FOR seq_110 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 208 + FORWARD PRIMER 69 20 59.82 55.00 GCGCTTACGAGCGTAGTTCT + + REVERSE PRIMER 257 20 59.79 55.00 ACTTGACGTACTCGCCCTGT + + + 2 PRODUCT SIZE: 221 + FORWARD PRIMER 69 20 59.82 55.00 GCGCTTACGAGCGTAGTTCT + + REVERSE PRIMER 270 20 59.84 50.00 TCATCGTGCAGGAACTTGAC + + + + +# EPRIMER3 RESULTS FOR seq_111 + +# Start Len Tm GC% Sequence + + + + + +# EPRIMER3 RESULTS FOR seq_112 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 206 + FORWARD PRIMER 17 19 59.02 52.63 GTGTTGTTGCTGTCGCTTG + + REVERSE PRIMER 203 20 61.13 55.00 ACGTTGCGGATGTAGTCGTC + + + 2 PRODUCT SIZE: 205 + FORWARD PRIMER 17 19 59.02 52.63 GTGTTGTTGCTGTCGCTTG + + REVERSE PRIMER 203 19 60.28 57.90 CGTTGCGGATGTAGTCGTC + + + + +# EPRIMER3 RESULTS FOR seq_113 + +# Start Len Tm GC% Sequence + + + + + +# EPRIMER3 RESULTS FOR seq_114 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 214 + FORWARD PRIMER 8 20 60.16 60.00 GCGAGAAGCAGGTACTGGAG + + REVERSE PRIMER 202 20 60.26 50.00 ACTGGATTTCTTGCCAGTGC + + + 2 PRODUCT SIZE: 225 + FORWARD PRIMER 8 20 60.16 60.00 GCGAGAAGCAGGTACTGGAG + + REVERSE PRIMER 213 20 59.06 50.00 GGGTCGCTGTAACTGGATTT + + + + +# EPRIMER3 RESULTS FOR seq_115 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 210 + FORWARD PRIMER 9 19 61.10 63.16 GTGAGCCAGCCGCTAGAAC + + REVERSE PRIMER 201 18 61.74 61.11 GATGCGGCCCTTCACTTC + + + 2 PRODUCT SIZE: 208 + FORWARD PRIMER 11 19 61.23 63.16 GAGCCAGCCGCTAGAACAG + + REVERSE PRIMER 201 18 61.74 61.11 GATGCGGCCCTTCACTTC + + + + +# EPRIMER3 RESULTS FOR seq_116 + +# Start Len Tm GC% Sequence + + + + + +# EPRIMER3 RESULTS FOR seq_117 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 202 + FORWARD PRIMER 66 20 58.83 45.00 CCGTTATCTTCGGAAAAAGC + + REVERSE PRIMER 248 20 60.45 45.00 TTTTATCCGTCACCCGCTTA + + + 2 PRODUCT SIZE: 203 + FORWARD PRIMER 65 20 58.83 45.00 GCCGTTATCTTCGGAAAAAG + + REVERSE PRIMER 248 20 60.45 45.00 TTTTATCCGTCACCCGCTTA + + + + +# EPRIMER3 RESULTS FOR seq_118 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 204 + FORWARD PRIMER 101 20 60.18 50.00 TGCCCCATACGGATCATACT + + REVERSE PRIMER 285 20 59.01 50.00 TACGACGGACAGAATGTGGT + + + 2 PRODUCT SIZE: 205 + FORWARD PRIMER 101 20 60.18 50.00 TGCCCCATACGGATCATACT + + REVERSE PRIMER 286 20 59.01 55.00 GTACGACGGACAGAATGTGG + + + + +# EPRIMER3 RESULTS FOR seq_119 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 205 + FORWARD PRIMER 113 20 59.86 50.00 TAGTGAGTCGATTGGCGTTG + + REVERSE PRIMER 298 20 59.96 55.00 GATTCCTTCGACGAGCTCAC + + + 2 PRODUCT SIZE: 204 + FORWARD PRIMER 114 20 60.72 50.00 AGTGAGTCGATTGGCGTTGT + + REVERSE PRIMER 298 20 59.96 55.00 GATTCCTTCGACGAGCTCAC + + + + +# EPRIMER3 RESULTS FOR seq_120 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 242 + FORWARD PRIMER 3 19 59.52 63.16 GCCTGGTATAGGCCCACTC + + REVERSE PRIMER 225 20 60.85 50.00 CCGGCGAACTATCCAATGTA + + + 2 PRODUCT SIZE: 242 + FORWARD PRIMER 3 19 59.52 63.16 GCCTGGTATAGGCCCACTC + + REVERSE PRIMER 226 19 60.87 52.63 CCGGCGAACTATCCAATGT + + + + +# EPRIMER3 RESULTS FOR seq_121 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 197 20 60.04 50.00 GGCCTGAAGATCGATACCAA + + REVERSE PRIMER 377 20 60.43 55.00 AGGTGCTGCTGGTGAATAGC + + + 2 PRODUCT SIZE: 202 + FORWARD PRIMER 197 20 60.04 50.00 GGCCTGAAGATCGATACCAA + + REVERSE PRIMER 379 20 60.41 50.00 TCAGGTGCTGCTGGTGAATA + + + + +# EPRIMER3 RESULTS FOR seq_122 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 208 + FORWARD PRIMER 28 19 59.97 52.63 AGAGCGCTGGATGGTCTTT + + REVERSE PRIMER 216 20 60.26 55.00 TTTCCAGGAGCGGACTACAC + + + 2 PRODUCT SIZE: 224 + FORWARD PRIMER 12 20 59.75 50.00 GATGCAGTGCTTTTGCAGAG + + REVERSE PRIMER 216 20 60.26 55.00 TTTCCAGGAGCGGACTACAC + + + + +# EPRIMER3 RESULTS FOR seq_123 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 177 20 59.69 50.00 AGATACCCGTCCTTCTGCAA + + REVERSE PRIMER 357 20 60.54 55.00 CCACTCGAAACCAACCAGAC + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 178 20 59.69 55.00 GATACCCGTCCTTCTGCAAG + + REVERSE PRIMER 358 20 60.54 50.00 ACCACTCGAAACCAACCAGA + + + + +# EPRIMER3 RESULTS FOR seq_124 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 216 + FORWARD PRIMER 97 20 60.26 55.00 ATACTGACTGGCCGCATAGC + + REVERSE PRIMER 293 20 60.02 55.00 ACATTGGCTACCGGTACTGC + + + 2 PRODUCT SIZE: 207 + FORWARD PRIMER 36 20 59.71 50.00 TATAAGTGCCTGCCATCGTG + + REVERSE PRIMER 223 20 60.46 50.00 CGGGCCAAAATGTCTATCTG + + + + +# EPRIMER3 RESULTS FOR seq_125 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 1 24 58.76 37.50 CCTTGAACGTCAGATAGACATTTT + + REVERSE PRIMER 183 18 62.27 61.11 GACGTCGGCGACAACCTT + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 1 23 57.45 39.13 CCTTGAACGTCAGATAGACATTT + + REVERSE PRIMER 183 18 62.27 61.11 GACGTCGGCGACAACCTT + + + + +# EPRIMER3 RESULTS FOR seq_126 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 226 + FORWARD PRIMER 108 20 59.62 55.00 CACAGCACTCTTTCGAGGTG + + REVERSE PRIMER 314 20 59.58 50.00 AGCTCCTCTTCGTTGAATGC + + + 2 PRODUCT SIZE: 228 + FORWARD PRIMER 108 20 59.62 55.00 CACAGCACTCTTTCGAGGTG + + REVERSE PRIMER 316 20 59.57 50.00 CGAGCTCCTCTTCGTTGAAT + + + + +# EPRIMER3 RESULTS FOR seq_127 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 203 + FORWARD PRIMER 332 20 59.97 45.00 AACAACCAAGATGGGCTTTG + + REVERSE PRIMER 515 20 60.31 55.00 CGCTCGTAGGTGTCAAAGGT + + + 2 PRODUCT SIZE: 201 + FORWARD PRIMER 151 20 59.54 45.00 TGAAATGCGGTTATCAGTGG + + REVERSE PRIMER 332 20 59.97 45.00 CAAAGCCCATCTTGGTTGTT + + + + +# EPRIMER3 RESULTS FOR seq_128 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 201 + FORWARD PRIMER 29 20 58.70 55.00 ATCTACCTGCGGGACGTACT + + REVERSE PRIMER 210 20 59.79 50.00 CGCATGGCAGTACACAACTT + + + 2 PRODUCT SIZE: 213 + FORWARD PRIMER 17 20 59.30 50.00 GGCGCAGAAATGATCTACCT + + REVERSE PRIMER 210 20 59.79 50.00 CGCATGGCAGTACACAACTT + + + + +# EPRIMER3 RESULTS FOR seq_129 + +# Start Len Tm GC% Sequence + + + + + +# EPRIMER3 RESULTS FOR seq_130 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 174 20 59.52 55.00 ACAGTCAGCAGCAGCAAGAG + + REVERSE PRIMER 354 20 59.75 50.00 AACGATGTCTTCGTGTGTCG + + + 2 PRODUCT SIZE: 203 + FORWARD PRIMER 171 20 60.50 55.00 GCTACAGTCAGCAGCAGCAA + + REVERSE PRIMER 354 20 59.75 50.00 AACGATGTCTTCGTGTGTCG + + + + +# EPRIMER3 RESULTS FOR seq_131 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 204 + FORWARD PRIMER 5 20 60.63 50.00 CCTGGATGCAGTGAAAAAGG + + REVERSE PRIMER 191 18 60.19 50.00 ATGGCGTCATCGAATGCT + + + 2 PRODUCT SIZE: 205 + FORWARD PRIMER 4 20 60.63 50.00 CCCTGGATGCAGTGAAAAAG + + REVERSE PRIMER 191 18 60.19 50.00 ATGGCGTCATCGAATGCT + + + + +# EPRIMER3 RESULTS FOR seq_132 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 454 20 60.14 55.00 ACATCGTCGCCCTCTACAAC + + REVERSE PRIMER 634 20 59.33 50.00 GAGTCGTTGGTGGCTTTTCT + + + 2 PRODUCT SIZE: 207 + FORWARD PRIMER 146 20 59.87 55.00 CTCAGCTGGAGGGTAAAACG + + REVERSE PRIMER 333 20 59.55 50.00 TGAGCCTGACTGAGGCAATA + + + + +# EPRIMER3 RESULTS FOR seq_133 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 204 + FORWARD PRIMER 29 20 59.01 55.00 CGGTCCAGATACTTGGGACT + + REVERSE PRIMER 212 21 60.32 42.86 CAAGTTATTGCCCGAATTCCT + + + 2 PRODUCT SIZE: 206 + FORWARD PRIMER 29 20 59.01 55.00 CGGTCCAGATACTTGGGACT + + REVERSE PRIMER 216 19 59.77 47.37 CCCAAGTTATTGCCCGAAT + + + + +# EPRIMER3 RESULTS FOR seq_134 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 226 + FORWARD PRIMER 15 20 59.90 55.00 GACTGGAACGCTCACACGTA + + REVERSE PRIMER 223 18 62.71 61.11 GCTCGGGATCCCCAAATC + + + 2 PRODUCT SIZE: 222 + FORWARD PRIMER 19 20 59.37 55.00 GGAACGCTCACACGTACAAG + + REVERSE PRIMER 223 18 62.71 61.11 GCTCGGGATCCCCAAATC + + + + +# EPRIMER3 RESULTS FOR seq_135 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 24 19 61.86 63.16 CCAGACCTTGACGCTGGAC + + REVERSE PRIMER 205 19 62.36 57.90 CCGGTGTTCCGAAGGAGAT + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 24 18 60.99 61.11 CCAGACCTTGACGCTGGA + + REVERSE PRIMER 205 19 62.36 57.90 CCGGTGTTCCGAAGGAGAT + + + + +# EPRIMER3 RESULTS FOR seq_136 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 201 + FORWARD PRIMER 8 19 59.87 57.90 ATACGCTCACTGCCACCTG + + REVERSE PRIMER 189 20 59.93 50.00 CCAAGTCGGATTGGTCTGAT + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 7 20 61.83 60.00 GATACGCTCACTGCCACCTG + + REVERSE PRIMER 187 20 60.08 50.00 AAGTCGGATTGGTCTGATGC + + + + +# EPRIMER3 RESULTS FOR seq_137 + +# Start Len Tm GC% Sequence + + + + + +# EPRIMER3 RESULTS FOR seq_138 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 205 + FORWARD PRIMER 65 19 60.17 52.63 CATCGATTGATGGGCTGAG + + REVERSE PRIMER 249 21 59.88 52.38 CGGGAGAGCTGTGTTGAATAG + + + 2 PRODUCT SIZE: 205 + FORWARD PRIMER 65 19 60.17 52.63 CATCGATTGATGGGCTGAG + + REVERSE PRIMER 250 20 58.87 50.00 CGGGAGAGCTGTGTTGAATA + + + + +# EPRIMER3 RESULTS FOR seq_139 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 19 20 59.94 50.00 TGCGACACGTCTTACACACA + + REVERSE PRIMER 199 20 59.60 50.00 GCGTGTGCTCCACACATATT + + + 2 PRODUCT SIZE: 205 + FORWARD PRIMER 14 20 60.33 55.00 CGTGATGCGACACGTCTTAC + + REVERSE PRIMER 199 20 59.60 50.00 GCGTGTGCTCCACACATATT + + + + +# EPRIMER3 RESULTS FOR seq_140 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 212 + FORWARD PRIMER 34 20 60.36 50.00 ATGAAGGAATGAGCCTGCTG + + REVERSE PRIMER 226 20 61.17 50.00 TGTGGAAAGCCTTGCGTATC + + + 2 PRODUCT SIZE: 212 + FORWARD PRIMER 34 20 60.36 50.00 ATGAAGGAATGAGCCTGCTG + + REVERSE PRIMER 227 19 59.29 47.37 TGTGGAAAGCCTTGCGTAT + + + + +# EPRIMER3 RESULTS FOR seq_141 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 208 + FORWARD PRIMER 530 20 60.40 55.00 TCGGCGTTCTGGTAGTTCTC + + REVERSE PRIMER 718 20 60.11 55.00 ACGATCTGGAGGTCTGGTTG + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 34 20 60.59 55.00 GGGTTTAGGTCGGGTGAAAC + + REVERSE PRIMER 214 20 59.67 55.00 ATAGCCTCCGGTTCTTCTCC + + + + +# EPRIMER3 RESULTS FOR seq_142 + +# Start Len Tm GC% Sequence + + + + + +# EPRIMER3 RESULTS FOR seq_143 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 202 + FORWARD PRIMER 17 20 60.87 50.00 GGACATCCCATTCGAGTTCA + + REVERSE PRIMER 199 20 60.06 55.00 CAGCGACGCTAGAGCTTCTT + + + 2 PRODUCT SIZE: 226 + FORWARD PRIMER 228 20 59.98 60.00 GCCTTAGCTCTCCCTCAGGT + + REVERSE PRIMER 434 20 59.97 50.00 AACTCGGAACCACGATCAAC + + + + +# EPRIMER3 RESULTS FOR seq_144 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 205 + FORWARD PRIMER 135 20 60.40 55.00 GAAGGGCGTCTCGTGAACTA + + REVERSE PRIMER 320 20 59.26 55.00 CCACGATCACCAGAGACTTG + + + 2 PRODUCT SIZE: 205 + FORWARD PRIMER 275 19 60.11 57.90 GTCGATGGACGTCGTGTTC + + REVERSE PRIMER 460 20 59.77 50.00 ACAGAACTCCTTGCCGTTGT + + + + +# EPRIMER3 RESULTS FOR seq_145 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 220 + FORWARD PRIMER 57 20 59.96 50.00 CCGAACCAGAATGCCTATGT + + REVERSE PRIMER 257 20 59.93 50.00 TTTAGAGTCCGGGGTTGATG + + + 2 PRODUCT SIZE: 209 + FORWARD PRIMER 68 20 59.23 45.00 TGCCTATGTCGAATCCTTCA + + REVERSE PRIMER 257 20 59.93 50.00 TTTAGAGTCCGGGGTTGATG + + + + +# EPRIMER3 RESULTS FOR seq_146 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 13 20 59.50 45.00 GCGAAAACTGCCAGAAACTT + + REVERSE PRIMER 193 20 60.41 60.00 CCCCTTCAGGTACCAGTGTG + + + 2 PRODUCT SIZE: 202 + FORWARD PRIMER 13 20 59.50 45.00 GCGAAAACTGCCAGAAACTT + + REVERSE PRIMER 195 20 58.91 55.00 AACCCCTTCAGGTACCAGTG + + + + +# EPRIMER3 RESULTS FOR seq_147 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 200 + FORWARD PRIMER 91 20 60.07 55.00 CTCCACCGAGACCAGATCAT + + REVERSE PRIMER 272 19 61.24 63.16 CAGTACGTGGTGCCGTGAC + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 91 20 60.07 55.00 CTCCACCGAGACCAGATCAT + + REVERSE PRIMER 273 18 60.32 61.11 CAGTACGTGGTGCCGTGA + + + + +# EPRIMER3 RESULTS FOR seq_148 + +# Start Len Tm GC% Sequence + + 1 PRODUCT SIZE: 203 + FORWARD PRIMER 79 20 59.81 50.00 AACCACCATATCCAGCAAGG + + REVERSE PRIMER 263 19 61.27 57.90 CATGTGACCGCCTGCTATG + + + 2 PRODUCT SIZE: 200 + FORWARD PRIMER 82 20 59.52 50.00 CACCATATCCAGCAAGGTGA + + REVERSE PRIMER 263 19 61.27 57.90 CATGTGACCGCCTGCTATG + + +