comparison uniqprimer-0.5.0/tmpz0OJOq/referenceprimers.ep3 @ 0:cdd8f911ad91 draft

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0:cdd8f911ad91

# EPRIMER3 RESULTS FOR seq_0

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 207
     FORWARD PRIMER       2   20  58.89  55.00  GATTAGCCCTGACCTTCAGC

     REVERSE PRIMER     189   20  60.21  55.00  CTGATTAGCCCTGACCCTCA


   2 PRODUCT SIZE: 205
     FORWARD PRIMER       2   20  58.89  55.00  GATTAGCCCTGACCTTCAGC

     REVERSE PRIMER     187   20  61.13  60.00  GATTAGCCCTGACCCTCAGC




# EPRIMER3 RESULTS FOR seq_1

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 202
     FORWARD PRIMER     211   20  60.49  50.00  CCAGTGATTTTCCGAAGTGG

     REVERSE PRIMER     393   20  59.85  55.00  TCTGACCGGCTGTATCACTG


   2 PRODUCT SIZE: 203
     FORWARD PRIMER     210   20  60.49  50.00  CCCAGTGATTTTCCGAAGTG

     REVERSE PRIMER     393   20  59.85  55.00  TCTGACCGGCTGTATCACTG




# EPRIMER3 RESULTS FOR seq_2

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 223
     FORWARD PRIMER     515   20  60.13  55.00  AACTGGACGATGCCTACCTG

     REVERSE PRIMER     718   20  59.71  55.00  GCACCGAGTCCATCACTGTA


   2 PRODUCT SIZE: 224
     FORWARD PRIMER     514   20  60.13  55.00  CAACTGGACGATGCCTACCT

     REVERSE PRIMER     718   20  59.71  55.00  GCACCGAGTCCATCACTGTA




# EPRIMER3 RESULTS FOR seq_3

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 230
     FORWARD PRIMER      13   20  60.07  50.00  CATCCTTCAGCCATGGAAGT

     REVERSE PRIMER     223   20  60.01  50.00  GTTGGTTGAATGTGGTGCTG


   2 PRODUCT SIZE: 216
     FORWARD PRIMER     151   20  60.43  55.00  CAGGTAGCGCCTTGCATACT

     REVERSE PRIMER     347   20  60.41  60.00  GAGCCGGAGTGTGAGGTCTA




# EPRIMER3 RESULTS FOR seq_4

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER      12   20  60.30  50.00  ACGAACGGCTGTTTGTTCTC

     REVERSE PRIMER     193   19  59.72  57.90  GCAGCACTGGAGTTGAAGC


   2 PRODUCT SIZE: 201
     FORWARD PRIMER      12   20  60.30  50.00  ACGAACGGCTGTTTGTTCTC

     REVERSE PRIMER     194   19  59.56  57.90  GGCAGCACTGGAGTTGAAG




# EPRIMER3 RESULTS FOR seq_5

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 220
     FORWARD PRIMER       5   20  60.30  55.00  CTTGCTGGAGGTCAACAGGT

     REVERSE PRIMER     205   20  60.26  55.00  GTGTTACCGGGCGTTGTATC


   2 PRODUCT SIZE: 221
     FORWARD PRIMER      18   20  60.55  50.00  AACAGGTACATCGCCTGCAT

     REVERSE PRIMER     219   20  60.11  50.00  GGAACCGAAGCCAAGTGTTA




# EPRIMER3 RESULTS FOR seq_6

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER      90   20  59.67  55.00  ATAGCCTCCGGTTCTTCTCC

     REVERSE PRIMER     270   20  60.59  55.00  GGGTTTAGGTCGGGTGAAAC


   2 PRODUCT SIZE: 202
     FORWARD PRIMER      88   20  60.59  55.00  CCATAGCCTCCGGTTCTTCT

     REVERSE PRIMER     270   20  60.59  55.00  GGGTTTAGGTCGGGTGAAAC




# EPRIMER3 RESULTS FOR seq_7

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 211
     FORWARD PRIMER      66   20  59.99  55.00  TGAGCCAGAGGTTCAGGTCT

     REVERSE PRIMER     257   20  60.34  50.00  CAACACGCAAGGTTGTGAAG


   2 PRODUCT SIZE: 212
     FORWARD PRIMER      65   20  59.99  60.00  CTGAGCCAGAGGTTCAGGTC

     REVERSE PRIMER     257   20  60.34  50.00  CAACACGCAAGGTTGTGAAG




# EPRIMER3 RESULTS FOR seq_8

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 201
     FORWARD PRIMER     195   20  59.76  60.00  CTGTCGGGTAGTAGCGGAAC

     REVERSE PRIMER     376   20  60.33  50.00  TTTTCTCTGGCAGGAGGATG


   2 PRODUCT SIZE: 206
     FORWARD PRIMER     455   20  59.60  45.00  TCCATCATCAATCGTCTTCG

     REVERSE PRIMER     641   20  60.01  55.00  AGGACACGTGGAGGTTGTTC




# EPRIMER3 RESULTS FOR seq_9

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 202
     FORWARD PRIMER     155   20  60.05  50.00  GATGGACCGGGTAAAAACCT

     REVERSE PRIMER     337   20  59.59  50.00  TCGAAGTTGTCGTAGCTCCA


   2 PRODUCT SIZE: 203
     FORWARD PRIMER     154   20  60.05  50.00  AGATGGACCGGGTAAAAACC

     REVERSE PRIMER     337   20  59.59  50.00  TCGAAGTTGTCGTAGCTCCA




# EPRIMER3 RESULTS FOR seq_10

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     383   20  59.60  45.00  TCAATCAAGTGCGCTGCTAT

     REVERSE PRIMER     563   20  60.15  55.00  CGGCTACTACATTGGCAGGT


   2 PRODUCT SIZE: 201
     FORWARD PRIMER     382   20  59.60  45.00  ATCAATCAAGTGCGCTGCTA

     REVERSE PRIMER     563   20  60.15  55.00  CGGCTACTACATTGGCAGGT




# EPRIMER3 RESULTS FOR seq_11

#                      Start  Len   Tm     GC%   Sequence





# EPRIMER3 RESULTS FOR seq_12

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 202
     FORWARD PRIMER     862   20  60.43  55.00  GTACAGATCGCCGAGCACTT

     REVERSE PRIMER    1044   20  60.24  50.00  CCAATCCAGACTTGCGCTAT


   2 PRODUCT SIZE: 201
     FORWARD PRIMER     862   20  60.43  55.00  GTACAGATCGCCGAGCACTT

     REVERSE PRIMER    1043   20  59.44  50.00  CAATCCAGACTTGCGCTATG




# EPRIMER3 RESULTS FOR seq_13

#                      Start  Len   Tm     GC%   Sequence





# EPRIMER3 RESULTS FOR seq_14

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 220
     FORWARD PRIMER      32   20  60.01  50.00  AGAGTAGAAGCGGCAACCAA

     REVERSE PRIMER     232   20  59.77  55.00  CGAAGAAGCGTGAGTGTGAG


   2 PRODUCT SIZE: 221
     FORWARD PRIMER      31   20  60.01  50.00  AAGAGTAGAAGCGGCAACCA

     REVERSE PRIMER     232   20  59.77  55.00  CGAAGAAGCGTGAGTGTGAG




# EPRIMER3 RESULTS FOR seq_15

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 226
     FORWARD PRIMER      13   19  59.71  57.90  GCGAGTAAGCCAGCAGATG

     REVERSE PRIMER     219   20  60.81  45.00  TTCATGCATCTACGCAAACG


   2 PRODUCT SIZE: 227
     FORWARD PRIMER      12   19  60.52  57.90  GGCGAGTAAGCCAGCAGAT

     REVERSE PRIMER     219   20  60.81  45.00  TTCATGCATCTACGCAAACG




# EPRIMER3 RESULTS FOR seq_16

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 216
     FORWARD PRIMER      53   20  59.99  55.00  ACTGAAGTCCTCCCTCAGCA

     REVERSE PRIMER     251   18  59.85  55.56  GAAACCGGGATGTCGATG


   2 PRODUCT SIZE: 217
     FORWARD PRIMER      53   20  59.99  55.00  ACTGAAGTCCTCCCTCAGCA

     REVERSE PRIMER     252   18  59.85  50.00  TGAAACCGGGATGTCGAT




# EPRIMER3 RESULTS FOR seq_17

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 206
     FORWARD PRIMER       3   21  59.83  42.86  TCGATTCAAAGCCTTACCTGA

     REVERSE PRIMER     189   20  58.35  45.00  GCTGGATTTTTGTTGCAGTC


   2 PRODUCT SIZE: 207
     FORWARD PRIMER       3   21  59.83  42.86  TCGATTCAAAGCCTTACCTGA

     REVERSE PRIMER     189   21  59.37  42.86  AGCTGGATTTTTGTTGCAGTC




# EPRIMER3 RESULTS FOR seq_18

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     836   19  59.91  57.90  ATCTGTCCGGGTTGGACTC

     REVERSE PRIMER    1016   20  59.85  55.00  GGTCTTGAGCACTTCCGAAC


   2 PRODUCT SIZE: 209
     FORWARD PRIMER    1120   20  60.94  55.00  CAGATCATGTACCCGGTGCT

     REVERSE PRIMER    1309   20  59.98  50.00  GCATCGCTGATGAAGTCGTA




# EPRIMER3 RESULTS FOR seq_19

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 202
     FORWARD PRIMER    1286   20  59.21  55.00  CAGTAGCAACCCCTTCAGGT

     REVERSE PRIMER    1468   20  60.10  50.00  CGTTCGGGTTCTTAGTTCCA


   2 PRODUCT SIZE: 217
     FORWARD PRIMER    1373   20  60.16  50.00  AAAACGCACTTCCAGTGACC

     REVERSE PRIMER    1570   20  60.01  55.00  AGAGAGACGGACGCTTGGTA




# EPRIMER3 RESULTS FOR seq_20

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     554   20  59.93  50.00  GGACAGCCCTGTATGGAAAA

     REVERSE PRIMER     734   20  60.97  50.00  TGGAAGAAGCAGTTGCCAGT


   2 PRODUCT SIZE: 209
     FORWARD PRIMER     554   20  59.93  50.00  GGACAGCCCTGTATGGAAAA

     REVERSE PRIMER     743   20  60.68  55.00  GGAGCTGACTGGAAGAAGCA




# EPRIMER3 RESULTS FOR seq_21

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 205
     FORWARD PRIMER     360   20  60.16  55.00  AAGCAGGTCTGGAACACCAC

     REVERSE PRIMER     545   20  60.83  55.00  GGAGTGCGTTAAAGCAGGTG


   2 PRODUCT SIZE: 208
     FORWARD PRIMER     200   20  60.37  45.00  TTTTTCCTGCACGGTGGTAT

     REVERSE PRIMER     388   20  59.44  50.00  TCCGTACTGGCAGTTCTTCA




# EPRIMER3 RESULTS FOR seq_22

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 213
     FORWARD PRIMER    1197   20  59.97  50.00  GCTTTGACCTTGTGGTCCAT

     REVERSE PRIMER    1390   20  59.85  55.00  CCAGATCAGCGTCAGTGGTA


   2 PRODUCT SIZE: 205
     FORWARD PRIMER    1557   20  59.86  50.00  CAAATGAGACGTCTGCGGTA

     REVERSE PRIMER    1742   20  60.45  50.00  TGCCTTTGTAGCACTGTGGA




# EPRIMER3 RESULTS FOR seq_23

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 204
     FORWARD PRIMER     109   20  59.68  50.00  CGTCATCAAACCAGACCTGA

     REVERSE PRIMER     293   20  59.98  50.00  TGGACTAGAAGCGCCTTGAT


   2 PRODUCT SIZE: 213
     FORWARD PRIMER     178   20  59.90  50.00  AAACGCTAAGCCGGTACTGA

     REVERSE PRIMER     371   20  60.29  55.00  GTGATGATGTCGAGCGTGTC




# EPRIMER3 RESULTS FOR seq_24

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 203
     FORWARD PRIMER      20   20  59.22  50.00  CTGAAGGCAACGTTCTGCTA

     REVERSE PRIMER     204   19  59.88  52.63  GTCTCCCATTTGCCCTGAT


   2 PRODUCT SIZE: 201
     FORWARD PRIMER      22   20  60.96  55.00  GAAGGCAACGTTCTGCTACG

     REVERSE PRIMER     204   19  59.88  52.63  GTCTCCCATTTGCCCTGAT




# EPRIMER3 RESULTS FOR seq_25

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 235
     FORWARD PRIMER      82   20  60.65  55.00  CGCAAGGTATCCAGTTCCTG

     REVERSE PRIMER     297   20  59.99  60.00  GGACCTCAGTGAAGGAGCAG


   2 PRODUCT SIZE: 239
     FORWARD PRIMER      87   20  59.31  55.00  GGTATCCAGTTCCTGCTGCT

     REVERSE PRIMER     306   20  60.32  60.00  AGCACTACCGGACCTCAGTG




# EPRIMER3 RESULTS FOR seq_26

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 202
     FORWARD PRIMER     173   20  60.24  50.00  CGCAGCTGGTATGGATTTCT

     REVERSE PRIMER     355   20  60.04  50.00  ATCACAGCGCACAGCATAAG


   2 PRODUCT SIZE: 205
     FORWARD PRIMER     409   20  60.17  55.00  CGGACATGTACGACGAGTTG

     REVERSE PRIMER     594   20  59.94  50.00  GACTCCCAAACCTGGAACAA




# EPRIMER3 RESULTS FOR seq_27

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER    1945   20  60.29  50.00  GTCACGATCCCGAATCAATC

     REVERSE PRIMER    2125   20  60.00  55.00  GACAGTTCTTCGGCGTTCTC


   2 PRODUCT SIZE: 200
     FORWARD PRIMER    6075   20  60.10  50.00  TGGCGACTATGTCGGATGTA

     REVERSE PRIMER    6255   20  60.24  50.00  GATGGTAGCGCGATGACTTT




# EPRIMER3 RESULTS FOR seq_28

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 205
     FORWARD PRIMER     350   20  60.25  50.00  TCAACGCCCTTCTTTACGTC

     REVERSE PRIMER     535   20  60.12  55.00  ACCTTGATGCTGGTGGAGTC


   2 PRODUCT SIZE: 209
     FORWARD PRIMER     321   20  59.97  50.00  CAATGTCAGCATGACCAACC

     REVERSE PRIMER     510   20  60.21  45.00  AGACCGCTTCGATTTTGATG




# EPRIMER3 RESULTS FOR seq_29

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 201
     FORWARD PRIMER     278   20  60.33  50.00  TTTTCTCTGGCAGGAGGATG

     REVERSE PRIMER     459   20  59.76  60.00  CTGTCGGGTAGTAGCGGAAC


   2 PRODUCT SIZE: 211
     FORWARD PRIMER     534   20  60.10  50.00  TATGCCGTGGATGTAGACGA

     REVERSE PRIMER     725   20  59.98  50.00  TGATCTGCTTCCACAACTCG




# EPRIMER3 RESULTS FOR seq_30

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 202
     FORWARD PRIMER     588   20  59.90  55.00  ACAGCGGCTACACTGGAGAT

     REVERSE PRIMER     770   20  59.48  50.00  AAGCTGGTATTGAGCCTTCG


   2 PRODUCT SIZE: 200
     FORWARD PRIMER     556   20  60.59  55.00  GGGTTTAGGTCGGGTGAAAC

     REVERSE PRIMER     736   20  59.67  55.00  ATAGCCTCCGGTTCTTCTCC




# EPRIMER3 RESULTS FOR seq_31

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 205
     FORWARD PRIMER     592   20  60.25  50.00  TCAACGCCCTTCTTTACGTC

     REVERSE PRIMER     777   20  60.12  55.00  ACCTTGATGCTGGTGGAGTC


   2 PRODUCT SIZE: 201
     FORWARD PRIMER     421   20  59.55  40.00  AAAATCCATCGGCGAAGTAA

     REVERSE PRIMER     602   20  60.16  50.00  CATGCTCTGCGACGTAAAGA




# EPRIMER3 RESULTS FOR seq_32

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 205
     FORWARD PRIMER      93   20  60.30  50.00  GGATTGGTTGATCGATACGC

     REVERSE PRIMER     278   20  60.11  55.00  TCCCTAGCCGAAGAGCACTA


   2 PRODUCT SIZE: 213
     FORWARD PRIMER     251   20  59.96  50.00  GCGAGAACTTTCATCCGAAG

     REVERSE PRIMER     444   20  59.88  40.00  CTTACGACGCAAAATGCAAA




# EPRIMER3 RESULTS FOR seq_33

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 225
     FORWARD PRIMER     117   20  60.67  55.00  GCACCGACATGAAGTTCCTC

     REVERSE PRIMER     322   20  60.39  55.00  TCAGGCAGTTCTTCCAGGTC


   2 PRODUCT SIZE: 216
     FORWARD PRIMER      87   20  59.49  55.00  GGGAGGAGGTCTTCTTCCAT

     REVERSE PRIMER     282   21  60.01  47.62  CGTACTTGTCCAATGGATGCT




# EPRIMER3 RESULTS FOR seq_34

#                      Start  Len   Tm     GC%   Sequence





# EPRIMER3 RESULTS FOR seq_35

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER      37   20  59.22  55.00  AAGCTACTCGGTGGATACGG

     REVERSE PRIMER     217   20  61.15  50.00  AGTTCATCTGCCAGCCCTTT


   2 PRODUCT SIZE: 202
     FORWARD PRIMER      19   20  60.37  45.00  GACGGTCAAAAAGAGCGAAA

     REVERSE PRIMER     202   19  59.38  52.63  CTTTGTGCTGGGAATGCTC




# EPRIMER3 RESULTS FOR seq_36

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     212   20  60.43  50.00  CCTTGACCAGTTGCAGCTTT

     REVERSE PRIMER     392   20  59.36  55.00  GTTCCTGATCCTGGACAACC


   2 PRODUCT SIZE: 209
     FORWARD PRIMER      24   20  59.55  45.00  TTGCAAAGGACACCACAATC

     REVERSE PRIMER     213   20  59.63  50.00  CAAAGCTGCAACTGGTCAAG




# EPRIMER3 RESULTS FOR seq_37

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 223
     FORWARD PRIMER     137   20  59.82  50.00  GCTACATGAAACCGGGATGT

     REVERSE PRIMER     340   20  59.99  55.00  ACTGAAGTCCTCCCTCAGCA


   2 PRODUCT SIZE: 222
     FORWARD PRIMER     137   20  59.82  50.00  GCTACATGAAACCGGGATGT

     REVERSE PRIMER     339   20  60.13  60.00  CTGAAGTCCTCCCTCAGCAG




# EPRIMER3 RESULTS FOR seq_38

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 201
     FORWARD PRIMER      52   20  59.99  45.00  CGTTGCGAATCAGAGTTGAA

     REVERSE PRIMER     233   20  59.78  40.00  GTTTCCGGTTCCATCAAAAA


   2 PRODUCT SIZE: 217
     FORWARD PRIMER     290   20  59.97  45.00  TAGCCGCTTTATTCGATGCT

     REVERSE PRIMER     487   20  59.95  45.00  GGTTTTCCTGACGTTTTCCA




# EPRIMER3 RESULTS FOR seq_39

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 206
     FORWARD PRIMER     579   20  59.79  50.00  TTGGTCTGATGCATCTCAGG

     REVERSE PRIMER     765   20  60.26  55.00  CCAGACGAGACCACGATTCT


   2 PRODUCT SIZE: 209
     FORWARD PRIMER     579   20  59.79  50.00  TTGGTCTGATGCATCTCAGG

     REVERSE PRIMER     768   20  60.12  55.00  GTTCCAGACGAGACCACGAT




# EPRIMER3 RESULTS FOR seq_40

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 228
     FORWARD PRIMER     200   20  60.28  50.00  TGGTCTGAAACACGACCTGA

     REVERSE PRIMER     408   20  59.97  45.00  ACGACACGGGATTTTCAAAG


   2 PRODUCT SIZE: 229
     FORWARD PRIMER     199   20  59.72  50.00  TTGGTCTGAAACACGACCTG

     REVERSE PRIMER     408   20  59.97  45.00  ACGACACGGGATTTTCAAAG




# EPRIMER3 RESULTS FOR seq_41

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 202
     FORWARD PRIMER       8   20  59.87  45.00  CGCGTCGTTGATGAGTAAAA

     REVERSE PRIMER     190   20  60.12  55.00  GCGGAGCTATGAAAGTGAGC


   2 PRODUCT SIZE: 207
     FORWARD PRIMER       3   20  59.97  50.00  TAGATCGCGTCGTTGATGAG

     REVERSE PRIMER     190   20  60.12  55.00  GCGGAGCTATGAAAGTGAGC




# EPRIMER3 RESULTS FOR seq_42

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 209
     FORWARD PRIMER      62   20  61.45  50.00  CGCCAACTGAACGAAAGCTA

     REVERSE PRIMER     251   20  60.00  45.00  AAGCGGGGTTTTTCGTAACT


   2 PRODUCT SIZE: 206
     FORWARD PRIMER      35   20  61.12  55.00  ACCACCGTTCTAGTGCATCG

     REVERSE PRIMER     221   20  59.42  45.00  TGCAAAGGGAGCTTGTATGA




# EPRIMER3 RESULTS FOR seq_43

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 207
     FORWARD PRIMER       4   20  58.89  55.00  GATTAGCCCTGACCTTCAGC

     REVERSE PRIMER     191   20  60.21  55.00  CTGATTAGCCCTGACCCTCA


   2 PRODUCT SIZE: 210
     FORWARD PRIMER       1   20  58.74  55.00  CCTGATTAGCCCTGACCTTC

     REVERSE PRIMER     191   20  60.21  55.00  CTGATTAGCCCTGACCCTCA




# EPRIMER3 RESULTS FOR seq_44

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 201
     FORWARD PRIMER       4   19  59.45  57.90  GATTAGCCCCATCCCTCAG

     REVERSE PRIMER     187   18  57.88  55.56  ATCAGCGGGACCTACCAT


   2 PRODUCT SIZE: 200
     FORWARD PRIMER       5   19  61.33  57.90  ATTAGCCCCATCCCTCAGC

     REVERSE PRIMER     187   18  57.88  55.56  ATCAGCGGGACCTACCAT




# EPRIMER3 RESULTS FOR seq_45

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 228
     FORWARD PRIMER      45   20  59.23  60.00  CCGGCACTGGTAGTAGACCT

     REVERSE PRIMER     253   20  60.66  55.00  GACAATGACCCCAGCTCAAG


   2 PRODUCT SIZE: 212
     FORWARD PRIMER      17   19  61.13  52.63  CACGCAAGGTGGTTTGATG

     REVERSE PRIMER     209   20  59.60  50.00  CAATCTCGTGCAGTTTCAGC




# EPRIMER3 RESULTS FOR seq_46

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 202
     FORWARD PRIMER     977   20  59.35  55.00  ACAGGACAGAGCCTTTACCG

     REVERSE PRIMER    1159   20  60.13  50.00  TTACGCCACCCTTACTTTGC


   2 PRODUCT SIZE: 204
     FORWARD PRIMER     366   20  60.62  50.00  ACGTTGGATGCGATCCTAGA

     REVERSE PRIMER     550   20  59.94  45.00  GGCGAATTGTGAGGTGAAAT




# EPRIMER3 RESULTS FOR seq_47

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 204
     FORWARD PRIMER     382   20  59.81  45.00  ATAAGCGCATCCTTCCTCAA

     REVERSE PRIMER     566   20  60.39  45.00  TGCCGTCTTCTTCATGTTCA


   2 PRODUCT SIZE: 207
     FORWARD PRIMER     465   20  60.15  45.00  TGTCCGCAACCTGTTTACAA

     REVERSE PRIMER     652   20  60.30  50.00  GACGTTGTTGAACGCCTCTT




# EPRIMER3 RESULTS FOR seq_48

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER    1509   20  59.88  55.00  CGGTAGGCCCACTATGTTGT

     REVERSE PRIMER    1689   20  60.11  45.00  CTTGTTCTGCCCGTTTCAAT


   2 PRODUCT SIZE: 202
     FORWARD PRIMER    1244   20  59.74  55.00  CGAGACGGTGTAGTTGTGGA

     REVERSE PRIMER    1426   20  59.97  45.00  CCTGCAAACTGGGTTTCAAT




# EPRIMER3 RESULTS FOR seq_49

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 211
     FORWARD PRIMER      48   20  60.19  50.00  GAAACCCCTTGGTGCCTATT

     REVERSE PRIMER     240   19  60.10  42.10  AACGCGTTGTTTCAATCCA


   2 PRODUCT SIZE: 202
     FORWARD PRIMER      57   20  59.18  45.00  TGGTGCCTATTGTGCCTAAA

     REVERSE PRIMER     240   19  60.10  42.10  AACGCGTTGTTTCAATCCA




# EPRIMER3 RESULTS FOR seq_50

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 212
     FORWARD PRIMER     197   20  59.54  55.00  CTATAGCGGCCGTAGTTGCT

     REVERSE PRIMER     389   20  59.48  60.00  GGCTCTTCGAGCTAGTCAGC


   2 PRODUCT SIZE: 214
     FORWARD PRIMER     197   20  59.54  55.00  CTATAGCGGCCGTAGTTGCT

     REVERSE PRIMER     390   21  59.91  52.38  TTGGCTCTTCGAGCTAGTCAG




# EPRIMER3 RESULTS FOR seq_51

#                      Start  Len   Tm     GC%   Sequence





# EPRIMER3 RESULTS FOR seq_52

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 206
     FORWARD PRIMER     127   20  59.03  45.00  GCACTCAAACCCCTGATTTT

     REVERSE PRIMER     313   20  60.12  50.00  GGGTGATCAAGCGTCAGTTT


   2 PRODUCT SIZE: 201
     FORWARD PRIMER      45   20  58.53  50.00  CATTAGCTCACCGTCTTTCG

     REVERSE PRIMER     226   20  59.98  50.00  TGTCGAATCTGTGGCTGAAG




# EPRIMER3 RESULTS FOR seq_53

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 203
     FORWARD PRIMER      18   18  59.21  55.56  CTGTGCATTCGACGTGGT

     REVERSE PRIMER     202   19  60.41  57.90  GTCGCGTAAAGGGCAGAGT


   2 PRODUCT SIZE: 202
     FORWARD PRIMER      19   18  61.40  55.56  TGTGCATTCGACGTGGTG

     REVERSE PRIMER     202   19  60.41  57.90  GTCGCGTAAAGGGCAGAGT




# EPRIMER3 RESULTS FOR seq_54

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER      18   20  59.84  55.00  GCCTCGACTTTGGATAGTGC

     REVERSE PRIMER     198   20  60.23  55.00  CTCTGAACAACTCCCCCTGA


   2 PRODUCT SIZE: 203
     FORWARD PRIMER      15   20  59.98  55.00  GCTGCCTCGACTTTGGATAG

     REVERSE PRIMER     198   20  60.23  55.00  CTCTGAACAACTCCCCCTGA




# EPRIMER3 RESULTS FOR seq_55

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 215
     FORWARD PRIMER      61   20  60.10  50.00  GCATCCACACGCCTATTCTT

     REVERSE PRIMER     256   20  59.98  55.00  CTTGCTCTTCAAGGGGTCTG


   2 PRODUCT SIZE: 217
     FORWARD PRIMER      59   20  60.10  50.00  AAGCATCCACACGCCTATTC

     REVERSE PRIMER     256   20  59.98  55.00  CTTGCTCTTCAAGGGGTCTG




# EPRIMER3 RESULTS FOR seq_56

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER    2453   20  59.94  40.00  AACGCATCGGCAAAATAATC

     REVERSE PRIMER    2633   20  60.23  55.00  GTCTAACATGGGGGTTGTCG


   2 PRODUCT SIZE: 205
     FORWARD PRIMER    3301   20  60.01  55.00  GCGACCTGCTTTTAGACCTG

     REVERSE PRIMER    3486   20  60.03  50.00  GCAAAGTCCACTTGCACAGA




# EPRIMER3 RESULTS FOR seq_57

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     950   20  59.95  55.00  ATCGTCCTGACACCGGATAG

     REVERSE PRIMER    1130   20  59.62  45.00  CTGAACAACGCACCACAAAT


   2 PRODUCT SIZE: 206
     FORWARD PRIMER     595   20  59.79  50.00  TTGGTCTGATGCATCTCAGG

     REVERSE PRIMER     781   20  60.26  55.00  CCAGACGAGACCACGATTCT




# EPRIMER3 RESULTS FOR seq_58

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 216
     FORWARD PRIMER     279   20  60.29  55.00  CGCTAACTGCCGGACTGTAT

     REVERSE PRIMER     475   20  59.97  45.00  AAAGCAATTGGCGGTATGAC


   2 PRODUCT SIZE: 218
     FORWARD PRIMER     279   20  60.29  55.00  CGCTAACTGCCGGACTGTAT

     REVERSE PRIMER     477   20  60.10  45.00  AGAAAGCAATTGGCGGTATG




# EPRIMER3 RESULTS FOR seq_59

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     448   20  59.27  45.00  ATCGAACATGGCCTGGTAAT

     REVERSE PRIMER     628   20  58.90  45.00  TGGAAACCTTCAAGTTGCTG


   2 PRODUCT SIZE: 201
     FORWARD PRIMER     448   20  59.27  45.00  ATCGAACATGGCCTGGTAAT

     REVERSE PRIMER     628   21  59.77  47.62  GTGGAAACCTTCAAGTTGCTG




# EPRIMER3 RESULTS FOR seq_60

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 226
     FORWARD PRIMER       6   20  59.33  60.00  GTAGCACTCCTCGGTCCACT

     REVERSE PRIMER     213   19  60.34  52.63  CGCTAATCCGCATCATCTG


   2 PRODUCT SIZE: 225
     FORWARD PRIMER       6   20  59.33  60.00  GTAGCACTCCTCGGTCCACT

     REVERSE PRIMER     212   19  59.35  52.63  GCTAATCCGCATCATCTGC




# EPRIMER3 RESULTS FOR seq_61

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     183   20  60.25  45.00  ACGACGAATTGGAAAAGCTG

     REVERSE PRIMER     363   20  60.10  50.00  CTGGCGTAGACGGAAATGAT


   2 PRODUCT SIZE: 201
     FORWARD PRIMER     351   20  59.89  50.00  TGGTGGAGGGCTATCATTTC

     REVERSE PRIMER     532   20  59.67  45.00  AAATAAGGGCATGCAGAGGA




# EPRIMER3 RESULTS FOR seq_62

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 202
     FORWARD PRIMER    1087   20  59.82  50.00  TCTACGCCGATGAAGTGATG

     REVERSE PRIMER    1269   20  60.14  55.00  ACCAGGCACAGCTGGTAATC


   2 PRODUCT SIZE: 203
     FORWARD PRIMER    1082   20  59.83  55.00  GGCTATCTACGCCGATGAAG

     REVERSE PRIMER    1265   20  60.14  55.00  GGCACAGCTGGTAATCCAGT




# EPRIMER3 RESULTS FOR seq_63

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 206
     FORWARD PRIMER      71   20  60.70  50.00  TGGCTCGGCCATCTCTATAA

     REVERSE PRIMER     257   20  60.07  50.00  ACTTCCATGGCTGAAGGATG


   2 PRODUCT SIZE: 223
     FORWARD PRIMER      39   20  60.07  50.00  CCAACATCCTTCCAGCCTAA

     REVERSE PRIMER     242   20  60.12  50.00  GGATGGGGCTAATCAGGAAT




# EPRIMER3 RESULTS FOR seq_64

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 215
     FORWARD PRIMER       5   20  61.91  55.00  CGATCCATGAACTCGCTCAG

     REVERSE PRIMER     200   20  58.89  40.00  CGCAAGTCTCATTGGAAAAA


   2 PRODUCT SIZE: 216
     FORWARD PRIMER       5   20  61.91  55.00  CGATCCATGAACTCGCTCAG

     REVERSE PRIMER     201   20  61.14  45.00  CCGCAAGTCTCATTGGAAAA




# EPRIMER3 RESULTS FOR seq_65

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 205
     FORWARD PRIMER     738   20  59.85  45.00  TCCAAAGCGTCTGGCTTTAT

     REVERSE PRIMER     923   20  60.10  55.00  TTGGCCGTAGAGGTATCGTC


   2 PRODUCT SIZE: 208
     FORWARD PRIMER     100   20  59.96  45.00  AAAGGCCAGCATCAAGAAGA

     REVERSE PRIMER     288   20  60.22  45.00  TTGGCCTTTCAAAGAAGGTG




# EPRIMER3 RESULTS FOR seq_66

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 203
     FORWARD PRIMER     861   20  59.89  50.00  ATGGTCGATGACGAGGATTC

     REVERSE PRIMER    1044   20  60.38  50.00  TCGGTTCTGTTTCAGGCTTC


   2 PRODUCT SIZE: 204
     FORWARD PRIMER     860   20  59.89  50.00  GATGGTCGATGACGAGGATT

     REVERSE PRIMER    1044   20  60.38  50.00  TCGGTTCTGTTTCAGGCTTC




# EPRIMER3 RESULTS FOR seq_67

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 210
     FORWARD PRIMER       5   20  61.25  50.00  GTGCACTTCCGACAGCAAAT

     REVERSE PRIMER     195   20  60.51  50.00  CCGAGAAGTTGAGCAAGGAA


   2 PRODUCT SIZE: 213
     FORWARD PRIMER       2   19  59.85  52.63  ATTGTGCACTTCCGACAGC

     REVERSE PRIMER     195   20  60.51  50.00  CCGAGAAGTTGAGCAAGGAA




# EPRIMER3 RESULTS FOR seq_68

#                      Start  Len   Tm     GC%   Sequence





# EPRIMER3 RESULTS FOR seq_69

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 204
     FORWARD PRIMER     573   20  60.44  45.00  ATGGCCAATTCCAGAGCATA

     REVERSE PRIMER     757   20  60.00  50.00  CCAACACAGTTTCGGATGTG


   2 PRODUCT SIZE: 212
     FORWARD PRIMER     284   20  60.04  50.00  AAGGCAAGTACGCAGAGCAT

     REVERSE PRIMER     476   20  59.55  55.00  CCCAAGGACAGGATAGTTGC




# EPRIMER3 RESULTS FOR seq_70

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER    4307   20  60.29  55.00  CCAGCGTACTGATGTCGATG

     REVERSE PRIMER    4487   20  60.03  45.00  CTTCGACAAAACGCACTCAA


   2 PRODUCT SIZE: 200
     FORWARD PRIMER    2300   20  59.89  55.00  CAGCTGATGCTCAAGCAGAC

     REVERSE PRIMER    2480   20  60.34  55.00  GAATAGGGCAGGGTGATGTG




# EPRIMER3 RESULTS FOR seq_71

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 201
     FORWARD PRIMER    1083   20  60.09  55.00  CAACCACCTACCCATCCAAC

     REVERSE PRIMER    1265   19  59.97  52.63  ATGCTCATGCCTTGCAGAC


   2 PRODUCT SIZE: 203
     FORWARD PRIMER    1081   20  60.05  50.00  ATCAACCACCTACCCATCCA

     REVERSE PRIMER    1265   19  59.97  52.63  ATGCTCATGCCTTGCAGAC




# EPRIMER3 RESULTS FOR seq_72

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 202
     FORWARD PRIMER     861   20  59.02  55.00  GGCACTACCTGGGTGAAGAT

     REVERSE PRIMER    1043   20  59.92  50.00  GCAGGATGTAGGGCATGATT


   2 PRODUCT SIZE: 201
     FORWARD PRIMER     590   19  59.94  52.63  GTATTACTCCGGCGCCATT

     REVERSE PRIMER     771   20  60.08  45.00  ATGAACTTGCCTTCCATTGC




# EPRIMER3 RESULTS FOR seq_73

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 201
     FORWARD PRIMER     378   20  60.63  55.00  GAGCCCATCAGTTCGATGAC

     REVERSE PRIMER     559   20  60.19  50.00  TGGCTGAAGGATTTCCAGAC


   2 PRODUCT SIZE: 202
     FORWARD PRIMER     378   20  60.63  55.00  GAGCCCATCAGTTCGATGAC

     REVERSE PRIMER     560   20  60.19  50.00  GTGGCTGAAGGATTTCCAGA




# EPRIMER3 RESULTS FOR seq_74

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 222
     FORWARD PRIMER     277   20  59.93  45.00  ACGAATGGATCGAAAACTGG

     REVERSE PRIMER     479   20  59.86  55.00  CCGAACTGAGTGTCCACAGA


   2 PRODUCT SIZE: 226
     FORWARD PRIMER     273   20  60.04  45.00  GGCTACGAATGGATCGAAAA

     REVERSE PRIMER     479   20  59.86  55.00  CCGAACTGAGTGTCCACAGA




# EPRIMER3 RESULTS FOR seq_75

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER    4903   20  60.41  55.00  CTCGCTGGAAGTGGATGAGT

     REVERSE PRIMER    5083   20  60.08  50.00  GAGATGGCGATGTTGAAGGT


   2 PRODUCT SIZE: 201
     FORWARD PRIMER    5164   20  59.56  50.00  ATCGTGCTGTTGATGACCAC

     REVERSE PRIMER    5345   20  59.53  50.00  GGTGATGACCACGAAGTTGA




# EPRIMER3 RESULTS FOR seq_76

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     141   20  61.37  55.00  CAACCTGGATGTGGTCTTGG

     REVERSE PRIMER     321   20  60.69  55.00  GTTCCAGTTCGGAGAACACG


   2 PRODUCT SIZE: 201
     FORWARD PRIMER     140   20  61.37  55.00  CCAACCTGGATGTGGTCTTG

     REVERSE PRIMER     321   20  60.69  55.00  GTTCCAGTTCGGAGAACACG




# EPRIMER3 RESULTS FOR seq_77

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     209   20  61.15  60.00  GCGACGTCTCGCTGAACTAC

     REVERSE PRIMER     389   20  60.26  45.00  GATGCGTTCGACGAAAAACT


   2 PRODUCT SIZE: 200
     FORWARD PRIMER     209   19  60.30  57.90  GCGACGTCTCGCTGAACTA

     REVERSE PRIMER     389   20  60.26  45.00  GATGCGTTCGACGAAAAACT




# EPRIMER3 RESULTS FOR seq_78

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 203
     FORWARD PRIMER    6514   20  59.98  50.00  CAGTCCTGTGATTTCGCTCA

     REVERSE PRIMER    6697   20  60.00  55.00  GTGAGCGGTGGAGTAATGGT


   2 PRODUCT SIZE: 200
     FORWARD PRIMER    3409   20  60.22  50.00  CTCCAGCGATCATTGAGGTT

     REVERSE PRIMER    3589   20  59.98  45.00  AATTCCAGCACACCGAAAAC




# EPRIMER3 RESULTS FOR seq_79

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 221
     FORWARD PRIMER     183   20  60.13  55.00  TCAGGAGCTGAAGTCGGAGT

     REVERSE PRIMER     384   20  59.79  50.00  AATCGAGTTGGGATGGTGAC


   2 PRODUCT SIZE: 201
     FORWARD PRIMER     234   19  59.96  52.63  GCGTGGTTTTCACCATCAC

     REVERSE PRIMER     415   20  59.61  55.00  TAAGCCTAGCCAGTCCGAAG




# EPRIMER3 RESULTS FOR seq_80

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 203
     FORWARD PRIMER    2488   20  60.09  60.00  CGTGGTCGTACGTACCTGTG

     REVERSE PRIMER    2671   20  59.99  55.00  AGGTCAGCCATTACCCACTG


   2 PRODUCT SIZE: 200
     FORWARD PRIMER     414   20  59.93  50.00  CAGGACCGGAACCTTCATAA

     REVERSE PRIMER     594   20  60.21  50.00  CTATGGCTTGACCCTCGAAA




# EPRIMER3 RESULTS FOR seq_81

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 204
     FORWARD PRIMER     448   20  60.07  45.00  GAGTTGTCGCCGGAAAAATA

     REVERSE PRIMER     632   20  59.46  45.00  GCTATTGGCAAAGCTTCGAT


   2 PRODUCT SIZE: 201
     FORWARD PRIMER     519   20  60.16  50.00  GCGATCGCCATCCTAGATAA

     REVERSE PRIMER     700   20  60.62  55.00  CCATAGGTGATGCCTTCTCG




# EPRIMER3 RESULTS FOR seq_82

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 207
     FORWARD PRIMER      45   20  59.79  55.00  ACGACGCAGTCCCTGTTAGT

     REVERSE PRIMER     232   20  59.70  50.00  GCGGAATTAGCGGTATCTTG


   2 PRODUCT SIZE: 203
     FORWARD PRIMER     305   20  59.83  50.00  GCAGGATTGTCATCAGCGTA

     REVERSE PRIMER     488   20  60.55  50.00  TATGATCCGTATGGGGCAAC




# EPRIMER3 RESULTS FOR seq_83

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 203
     FORWARD PRIMER    1191   20  60.09  60.00  CGTGGTCGTACGTACCTGTG

     REVERSE PRIMER    1374   20  59.99  55.00  AGGTCAGCCATTACCCACTG


   2 PRODUCT SIZE: 204
     FORWARD PRIMER    1191   20  60.09  60.00  CGTGGTCGTACGTACCTGTG

     REVERSE PRIMER    1375   20  59.99  55.00  CAGGTCAGCCATTACCCACT




# EPRIMER3 RESULTS FOR seq_84

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 216
     FORWARD PRIMER      83   20  60.26  55.00  ATACTGACTGGCCGCATAGC

     REVERSE PRIMER     279   20  60.02  55.00  ACATTGGCTACCGGTACTGC


   2 PRODUCT SIZE: 207
     FORWARD PRIMER      22   20  59.71  50.00  TATAAGTGCCTGCCATCGTG

     REVERSE PRIMER     209   20  60.46  50.00  CGGGCCAAAATGTCTATCTG




# EPRIMER3 RESULTS FOR seq_85

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 216
     FORWARD PRIMER      42   20  60.26  55.00  ATACTGACTGGCCGCATAGC

     REVERSE PRIMER     238   20  60.02  55.00  ACATTGGCTACCGGTACTGC


   2 PRODUCT SIZE: 214
     FORWARD PRIMER      42   20  60.26  55.00  ATACTGACTGGCCGCATAGC

     REVERSE PRIMER     236   20  60.15  55.00  ATTGGCTACCGGTACTGCTG




# EPRIMER3 RESULTS FOR seq_86

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER    5881   20  59.91  45.00  TTTTGGCTGTGCTTTACACG

     REVERSE PRIMER    6061   20  59.98  55.00  CGACATCACCCACTACATCG


   2 PRODUCT SIZE: 203
     FORWARD PRIMER    2699   20  60.03  60.00  CCGGGGTACTCAGTGTCAGT

     REVERSE PRIMER    2882   20  59.86  50.00  CAGTTCGTCCAATACGCAGA




# EPRIMER3 RESULTS FOR seq_87

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 207
     FORWARD PRIMER      87   20  59.87  55.00  CTCAGCTGGAGGGTAAAACG

     REVERSE PRIMER     274   20  59.55  50.00  TGAGCCTGACTGAGGCAATA


   2 PRODUCT SIZE: 208
     FORWARD PRIMER      87   20  59.87  55.00  CTCAGCTGGAGGGTAAAACG

     REVERSE PRIMER     275   20  60.56  55.00  CTGAGCCTGACTGAGGCAAT




# EPRIMER3 RESULTS FOR seq_88

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 211
     FORWARD PRIMER      42   20  60.43  60.00  CCCCTACACCCCTACAATCC

     REVERSE PRIMER     233   20  60.01  55.00  AGTAATCGTGGTGCCGGTAG


   2 PRODUCT SIZE: 210
     FORWARD PRIMER      42   20  60.43  60.00  CCCCTACACCCCTACAATCC

     REVERSE PRIMER     232   20  59.88  55.00  GTAATCGTGGTGCCGGTAGT




# EPRIMER3 RESULTS FOR seq_89

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 205
     FORWARD PRIMER     137   20  60.01  50.00  ACAGCCAAAAGTGGGTTCAC

     REVERSE PRIMER     322   20  60.68  55.00  ACATGGTCGCCTCCAGACTA


   2 PRODUCT SIZE: 206
     FORWARD PRIMER     137   20  60.01  50.00  ACAGCCAAAAGTGGGTTCAC

     REVERSE PRIMER     323   20  60.68  55.00  TACATGGTCGCCTCCAGACT




# EPRIMER3 RESULTS FOR seq_90

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER    3152   20  60.14  55.00  ACATCGTCGCCCTCTACAAC

     REVERSE PRIMER    3332   20  59.33  50.00  GAGTCGTTGGTGGCTTTTCT


   2 PRODUCT SIZE: 207
     FORWARD PRIMER    2844   20  59.87  55.00  CTCAGCTGGAGGGTAAAACG

     REVERSE PRIMER    3031   20  59.55  50.00  TGAGCCTGACTGAGGCAATA




# EPRIMER3 RESULTS FOR seq_91

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 207
     FORWARD PRIMER      66   20  60.06  50.00  GCGGGATGCACCTAAAGATA

     REVERSE PRIMER     254   19  60.68  57.90  ACTGGCGGAGAAGCTCAAG


   2 PRODUCT SIZE: 208
     FORWARD PRIMER      66   20  60.06  50.00  GCGGGATGCACCTAAAGATA

     REVERSE PRIMER     254   20  61.98  55.00  AACTGGCGGAGAAGCTCAAG




# EPRIMER3 RESULTS FOR seq_92

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 226
     FORWARD PRIMER      37   20  60.00  55.00  ACCGTGATTACCCTGCTCAC

     REVERSE PRIMER     243   20  59.81  50.00  ATACCACCGAATAGCGGATG


   2 PRODUCT SIZE: 231
     FORWARD PRIMER      37   20  60.00  55.00  ACCGTGATTACCCTGCTCAC

     REVERSE PRIMER     248   20  60.21  55.00  CCACCATACCACCGAATAGC




# EPRIMER3 RESULTS FOR seq_93

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     656   20  61.46  60.00  CTCGGGACGTTCCTACTGGT

     REVERSE PRIMER     836   20  60.00  45.00  ACGTAACGCACCGAAAAATC


   2 PRODUCT SIZE: 207
     FORWARD PRIMER     179   20  60.99  45.00  ATGGTCGAAAAAGGCTCGTT

     REVERSE PRIMER     366   20  60.13  50.00  ATAGGTTTCGTCGTGCTTGG




# EPRIMER3 RESULTS FOR seq_94

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     118   19  60.62  57.90  GAAGGTGGCGACCTCAATC

     REVERSE PRIMER     300   18  60.66  55.56  ATGCCAACCGTGAGCATC


   2 PRODUCT SIZE: 245
     FORWARD PRIMER      67   20  59.29  40.00  TTTTTGCTGTTCACCTCCAA

     REVERSE PRIMER     294   18  60.87  61.11  ACCGTGAGCATCGACCAG




# EPRIMER3 RESULTS FOR seq_95

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 204
     FORWARD PRIMER      21   21  59.99  47.62  TGGACCTTGTTACTGGCAATC

     REVERSE PRIMER     206   19  59.49  52.63  ATCATCCACCAGCGACAAC


   2 PRODUCT SIZE: 205
     FORWARD PRIMER      21   21  59.99  47.62  TGGACCTTGTTACTGGCAATC

     REVERSE PRIMER     206   20  61.51  55.00  GATCATCCACCAGCGACAAC




# EPRIMER3 RESULTS FOR seq_96

#                      Start  Len   Tm     GC%   Sequence





# EPRIMER3 RESULTS FOR seq_97

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 201
     FORWARD PRIMER     437   20  59.70  45.00  ATGTCGCGGAGAAACTTCAT

     REVERSE PRIMER     618   20  59.71  50.00  CCTGTGTGGGACGGTTTTAT


   2 PRODUCT SIZE: 200
     FORWARD PRIMER     266   21  60.33  52.38  ACCGACAGCTCGACATACTTG

     REVERSE PRIMER     446   20  60.12  50.00  GGCACCGACATGAAGTTTCT




# EPRIMER3 RESULTS FOR seq_98

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 205
     FORWARD PRIMER      72   20  61.04  50.00  CCGGAAAAATCGCTTAGGAG

     REVERSE PRIMER     257   20  59.56  55.00  GCGGATCTGGGTAGTGAAAC


   2 PRODUCT SIZE: 204
     FORWARD PRIMER      72   20  61.04  50.00  CCGGAAAAATCGCTTAGGAG

     REVERSE PRIMER     256   20  60.51  55.00  CGGATCTGGGTAGTGAAACG




# EPRIMER3 RESULTS FOR seq_99

#                      Start  Len   Tm     GC%   Sequence





# EPRIMER3 RESULTS FOR seq_100

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 214
     FORWARD PRIMER      26   18  60.65  55.56  GCGTCGCTTGATCGAAAG

     REVERSE PRIMER     220   20  59.87  50.00  GCACGGTGAGTTTGCTCATA


   2 PRODUCT SIZE: 220
     FORWARD PRIMER      20   18  60.82  55.56  GATGGTGCGTCGCTTGAT

     REVERSE PRIMER     220   20  59.87  50.00  GCACGGTGAGTTTGCTCATA




# EPRIMER3 RESULTS FOR seq_101

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 201
     FORWARD PRIMER       5   20  60.07  50.00  AAGTCATGCCAATCCTGGAG

     REVERSE PRIMER     187   19  61.35  52.63  ATCACCGCCATCCACTCAT


   2 PRODUCT SIZE: 204
     FORWARD PRIMER       2   20  60.07  50.00  AGGAAGTCATGCCAATCCTG

     REVERSE PRIMER     187   19  61.35  52.63  ATCACCGCCATCCACTCAT




# EPRIMER3 RESULTS FOR seq_102

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 203
     FORWARD PRIMER      55   19  61.88  57.90  GCAATAGCGAAGCGGTGAC

     REVERSE PRIMER     238   20  60.35  45.00  CAATTCGCTTTCCAGCAGAT


   2 PRODUCT SIZE: 205
     FORWARD PRIMER      55   19  61.88  57.90  GCAATAGCGAAGCGGTGAC

     REVERSE PRIMER     240   20  60.39  45.00  AACAATTCGCTTTCCAGCAG




# EPRIMER3 RESULTS FOR seq_103

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 206
     FORWARD PRIMER     129   20  60.10  55.00  GGAGATCTTTGAGCGTGAGC

     REVERSE PRIMER     315   20  60.47  50.00  CGATAGCACCGGTTTGAATC


   2 PRODUCT SIZE: 202
     FORWARD PRIMER      99   20  60.10  50.00  GCGGATGCACTCCAAGTATT

     REVERSE PRIMER     281   20  60.78  50.00  GAGGCAGACGTTTTCGAATG




# EPRIMER3 RESULTS FOR seq_104

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     172   20  59.46  45.00  GAGCAGTTTTCGGCAGATTT

     REVERSE PRIMER     352   20  60.25  50.00  TCTTCGTTTACCTCGGCAAC


   2 PRODUCT SIZE: 202
     FORWARD PRIMER     133   20  59.09  50.00  CTGTTCGTCAACATCGATCC

     REVERSE PRIMER     315   20  59.96  55.00  CTAAGGTCTTGGGGTGGACA




# EPRIMER3 RESULTS FOR seq_105

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 209
     FORWARD PRIMER      91   19  60.30  47.37  AATCCATGAATCGGCTGGT

     REVERSE PRIMER     280   20  60.48  55.00  GGATTCAGTCCATCCAGCAC


   2 PRODUCT SIZE: 212
     FORWARD PRIMER      91   19  60.30  47.37  AATCCATGAATCGGCTGGT

     REVERSE PRIMER     283   20  60.47  55.00  GTCGGATTCAGTCCATCCAG




# EPRIMER3 RESULTS FOR seq_106

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 203
     FORWARD PRIMER      39   20  59.99  55.00  CTACGGGACCTGACGTTCAT

     REVERSE PRIMER     222   20  61.13  55.00  ACATGGTGCAGTTCGGCTAC


   2 PRODUCT SIZE: 202
     FORWARD PRIMER      39   20  59.99  55.00  CTACGGGACCTGACGTTCAT

     REVERSE PRIMER     222   19  60.28  57.90  CATGGTGCAGTTCGGCTAC




# EPRIMER3 RESULTS FOR seq_107

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 234
     FORWARD PRIMER      49   20  60.14  60.00  GCTGGAGCAGTCAGGAGTTC

     REVERSE PRIMER     263   20  59.40  45.00  TCTGTGGGCCATAAATGTTG


   2 PRODUCT SIZE: 201
     FORWARD PRIMER      49   20  60.14  60.00  GCTGGAGCAGTCAGGAGTTC

     REVERSE PRIMER     230   20  62.27  50.00  GCGGTTGAAGCAGAAAAAGC




# EPRIMER3 RESULTS FOR seq_108

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 201
     FORWARD PRIMER     181   20  60.62  55.00  TATACGAGCCTGCCCAGGTA

     REVERSE PRIMER     362   20  60.05  45.00  TATCGGCGTATTCCGAAAAG


   2 PRODUCT SIZE: 200
     FORWARD PRIMER     182   20  60.87  55.00  ATACGAGCCTGCCCAGGTAT

     REVERSE PRIMER     362   20  60.05  45.00  TATCGGCGTATTCCGAAAAG




# EPRIMER3 RESULTS FOR seq_109

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 201
     FORWARD PRIMER      58   20  59.89  50.00  TGGTGGAGGGCTATCATTTC

     REVERSE PRIMER     239   20  59.67  45.00  AAATAAGGGCATGCAGAGGA


   2 PRODUCT SIZE: 205
     FORWARD PRIMER      51   20  59.93  50.00  GACAAATTGGTGGAGGGCTA

     REVERSE PRIMER     236   20  60.36  55.00  TAAGGGCATGCAGAGGAGTC




# EPRIMER3 RESULTS FOR seq_110

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 208
     FORWARD PRIMER      69   20  59.82  55.00  GCGCTTACGAGCGTAGTTCT

     REVERSE PRIMER     257   20  59.79  55.00  ACTTGACGTACTCGCCCTGT


   2 PRODUCT SIZE: 221
     FORWARD PRIMER      69   20  59.82  55.00  GCGCTTACGAGCGTAGTTCT

     REVERSE PRIMER     270   20  59.84  50.00  TCATCGTGCAGGAACTTGAC




# EPRIMER3 RESULTS FOR seq_111

#                      Start  Len   Tm     GC%   Sequence





# EPRIMER3 RESULTS FOR seq_112

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 206
     FORWARD PRIMER      17   19  59.02  52.63  GTGTTGTTGCTGTCGCTTG

     REVERSE PRIMER     203   20  61.13  55.00  ACGTTGCGGATGTAGTCGTC


   2 PRODUCT SIZE: 205
     FORWARD PRIMER      17   19  59.02  52.63  GTGTTGTTGCTGTCGCTTG

     REVERSE PRIMER     203   19  60.28  57.90  CGTTGCGGATGTAGTCGTC




# EPRIMER3 RESULTS FOR seq_113

#                      Start  Len   Tm     GC%   Sequence





# EPRIMER3 RESULTS FOR seq_114

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 214
     FORWARD PRIMER       8   20  60.16  60.00  GCGAGAAGCAGGTACTGGAG

     REVERSE PRIMER     202   20  60.26  50.00  ACTGGATTTCTTGCCAGTGC


   2 PRODUCT SIZE: 225
     FORWARD PRIMER       8   20  60.16  60.00  GCGAGAAGCAGGTACTGGAG

     REVERSE PRIMER     213   20  59.06  50.00  GGGTCGCTGTAACTGGATTT




# EPRIMER3 RESULTS FOR seq_115

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 210
     FORWARD PRIMER       9   19  61.10  63.16  GTGAGCCAGCCGCTAGAAC

     REVERSE PRIMER     201   18  61.74  61.11  GATGCGGCCCTTCACTTC


   2 PRODUCT SIZE: 208
     FORWARD PRIMER      11   19  61.23  63.16  GAGCCAGCCGCTAGAACAG

     REVERSE PRIMER     201   18  61.74  61.11  GATGCGGCCCTTCACTTC




# EPRIMER3 RESULTS FOR seq_116

#                      Start  Len   Tm     GC%   Sequence





# EPRIMER3 RESULTS FOR seq_117

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 202
     FORWARD PRIMER      66   20  58.83  45.00  CCGTTATCTTCGGAAAAAGC

     REVERSE PRIMER     248   20  60.45  45.00  TTTTATCCGTCACCCGCTTA


   2 PRODUCT SIZE: 203
     FORWARD PRIMER      65   20  58.83  45.00  GCCGTTATCTTCGGAAAAAG

     REVERSE PRIMER     248   20  60.45  45.00  TTTTATCCGTCACCCGCTTA




# EPRIMER3 RESULTS FOR seq_118

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 204
     FORWARD PRIMER     101   20  60.18  50.00  TGCCCCATACGGATCATACT

     REVERSE PRIMER     285   20  59.01  50.00  TACGACGGACAGAATGTGGT


   2 PRODUCT SIZE: 205
     FORWARD PRIMER     101   20  60.18  50.00  TGCCCCATACGGATCATACT

     REVERSE PRIMER     286   20  59.01  55.00  GTACGACGGACAGAATGTGG




# EPRIMER3 RESULTS FOR seq_119

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 205
     FORWARD PRIMER     113   20  59.86  50.00  TAGTGAGTCGATTGGCGTTG

     REVERSE PRIMER     298   20  59.96  55.00  GATTCCTTCGACGAGCTCAC


   2 PRODUCT SIZE: 204
     FORWARD PRIMER     114   20  60.72  50.00  AGTGAGTCGATTGGCGTTGT

     REVERSE PRIMER     298   20  59.96  55.00  GATTCCTTCGACGAGCTCAC




# EPRIMER3 RESULTS FOR seq_120

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 242
     FORWARD PRIMER       3   19  59.52  63.16  GCCTGGTATAGGCCCACTC

     REVERSE PRIMER     225   20  60.85  50.00  CCGGCGAACTATCCAATGTA


   2 PRODUCT SIZE: 242
     FORWARD PRIMER       3   19  59.52  63.16  GCCTGGTATAGGCCCACTC

     REVERSE PRIMER     226   19  60.87  52.63  CCGGCGAACTATCCAATGT




# EPRIMER3 RESULTS FOR seq_121

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     197   20  60.04  50.00  GGCCTGAAGATCGATACCAA

     REVERSE PRIMER     377   20  60.43  55.00  AGGTGCTGCTGGTGAATAGC


   2 PRODUCT SIZE: 202
     FORWARD PRIMER     197   20  60.04  50.00  GGCCTGAAGATCGATACCAA

     REVERSE PRIMER     379   20  60.41  50.00  TCAGGTGCTGCTGGTGAATA




# EPRIMER3 RESULTS FOR seq_122

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 208
     FORWARD PRIMER      28   19  59.97  52.63  AGAGCGCTGGATGGTCTTT

     REVERSE PRIMER     216   20  60.26  55.00  TTTCCAGGAGCGGACTACAC


   2 PRODUCT SIZE: 224
     FORWARD PRIMER      12   20  59.75  50.00  GATGCAGTGCTTTTGCAGAG

     REVERSE PRIMER     216   20  60.26  55.00  TTTCCAGGAGCGGACTACAC




# EPRIMER3 RESULTS FOR seq_123

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     177   20  59.69  50.00  AGATACCCGTCCTTCTGCAA

     REVERSE PRIMER     357   20  60.54  55.00  CCACTCGAAACCAACCAGAC


   2 PRODUCT SIZE: 200
     FORWARD PRIMER     178   20  59.69  55.00  GATACCCGTCCTTCTGCAAG

     REVERSE PRIMER     358   20  60.54  50.00  ACCACTCGAAACCAACCAGA




# EPRIMER3 RESULTS FOR seq_124

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 216
     FORWARD PRIMER      97   20  60.26  55.00  ATACTGACTGGCCGCATAGC

     REVERSE PRIMER     293   20  60.02  55.00  ACATTGGCTACCGGTACTGC


   2 PRODUCT SIZE: 207
     FORWARD PRIMER      36   20  59.71  50.00  TATAAGTGCCTGCCATCGTG

     REVERSE PRIMER     223   20  60.46  50.00  CGGGCCAAAATGTCTATCTG




# EPRIMER3 RESULTS FOR seq_125

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER       1   24  58.76  37.50  CCTTGAACGTCAGATAGACATTTT

     REVERSE PRIMER     183   18  62.27  61.11  GACGTCGGCGACAACCTT


   2 PRODUCT SIZE: 200
     FORWARD PRIMER       1   23  57.45  39.13  CCTTGAACGTCAGATAGACATTT

     REVERSE PRIMER     183   18  62.27  61.11  GACGTCGGCGACAACCTT




# EPRIMER3 RESULTS FOR seq_126

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 226
     FORWARD PRIMER     108   20  59.62  55.00  CACAGCACTCTTTCGAGGTG

     REVERSE PRIMER     314   20  59.58  50.00  AGCTCCTCTTCGTTGAATGC


   2 PRODUCT SIZE: 228
     FORWARD PRIMER     108   20  59.62  55.00  CACAGCACTCTTTCGAGGTG

     REVERSE PRIMER     316   20  59.57  50.00  CGAGCTCCTCTTCGTTGAAT




# EPRIMER3 RESULTS FOR seq_127

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 203
     FORWARD PRIMER     332   20  59.97  45.00  AACAACCAAGATGGGCTTTG

     REVERSE PRIMER     515   20  60.31  55.00  CGCTCGTAGGTGTCAAAGGT


   2 PRODUCT SIZE: 201
     FORWARD PRIMER     151   20  59.54  45.00  TGAAATGCGGTTATCAGTGG

     REVERSE PRIMER     332   20  59.97  45.00  CAAAGCCCATCTTGGTTGTT




# EPRIMER3 RESULTS FOR seq_128

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 201
     FORWARD PRIMER      29   20  58.70  55.00  ATCTACCTGCGGGACGTACT

     REVERSE PRIMER     210   20  59.79  50.00  CGCATGGCAGTACACAACTT


   2 PRODUCT SIZE: 213
     FORWARD PRIMER      17   20  59.30  50.00  GGCGCAGAAATGATCTACCT

     REVERSE PRIMER     210   20  59.79  50.00  CGCATGGCAGTACACAACTT




# EPRIMER3 RESULTS FOR seq_129

#                      Start  Len   Tm     GC%   Sequence





# EPRIMER3 RESULTS FOR seq_130

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     174   20  59.52  55.00  ACAGTCAGCAGCAGCAAGAG

     REVERSE PRIMER     354   20  59.75  50.00  AACGATGTCTTCGTGTGTCG


   2 PRODUCT SIZE: 203
     FORWARD PRIMER     171   20  60.50  55.00  GCTACAGTCAGCAGCAGCAA

     REVERSE PRIMER     354   20  59.75  50.00  AACGATGTCTTCGTGTGTCG




# EPRIMER3 RESULTS FOR seq_131

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 204
     FORWARD PRIMER       5   20  60.63  50.00  CCTGGATGCAGTGAAAAAGG

     REVERSE PRIMER     191   18  60.19  50.00  ATGGCGTCATCGAATGCT


   2 PRODUCT SIZE: 205
     FORWARD PRIMER       4   20  60.63  50.00  CCCTGGATGCAGTGAAAAAG

     REVERSE PRIMER     191   18  60.19  50.00  ATGGCGTCATCGAATGCT




# EPRIMER3 RESULTS FOR seq_132

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER     454   20  60.14  55.00  ACATCGTCGCCCTCTACAAC

     REVERSE PRIMER     634   20  59.33  50.00  GAGTCGTTGGTGGCTTTTCT


   2 PRODUCT SIZE: 207
     FORWARD PRIMER     146   20  59.87  55.00  CTCAGCTGGAGGGTAAAACG

     REVERSE PRIMER     333   20  59.55  50.00  TGAGCCTGACTGAGGCAATA




# EPRIMER3 RESULTS FOR seq_133

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 204
     FORWARD PRIMER      29   20  59.01  55.00  CGGTCCAGATACTTGGGACT

     REVERSE PRIMER     212   21  60.32  42.86  CAAGTTATTGCCCGAATTCCT


   2 PRODUCT SIZE: 206
     FORWARD PRIMER      29   20  59.01  55.00  CGGTCCAGATACTTGGGACT

     REVERSE PRIMER     216   19  59.77  47.37  CCCAAGTTATTGCCCGAAT




# EPRIMER3 RESULTS FOR seq_134

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 226
     FORWARD PRIMER      15   20  59.90  55.00  GACTGGAACGCTCACACGTA

     REVERSE PRIMER     223   18  62.71  61.11  GCTCGGGATCCCCAAATC


   2 PRODUCT SIZE: 222
     FORWARD PRIMER      19   20  59.37  55.00  GGAACGCTCACACGTACAAG

     REVERSE PRIMER     223   18  62.71  61.11  GCTCGGGATCCCCAAATC




# EPRIMER3 RESULTS FOR seq_135

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER      24   19  61.86  63.16  CCAGACCTTGACGCTGGAC

     REVERSE PRIMER     205   19  62.36  57.90  CCGGTGTTCCGAAGGAGAT


   2 PRODUCT SIZE: 200
     FORWARD PRIMER      24   18  60.99  61.11  CCAGACCTTGACGCTGGA

     REVERSE PRIMER     205   19  62.36  57.90  CCGGTGTTCCGAAGGAGAT




# EPRIMER3 RESULTS FOR seq_136

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 201
     FORWARD PRIMER       8   19  59.87  57.90  ATACGCTCACTGCCACCTG

     REVERSE PRIMER     189   20  59.93  50.00  CCAAGTCGGATTGGTCTGAT


   2 PRODUCT SIZE: 200
     FORWARD PRIMER       7   20  61.83  60.00  GATACGCTCACTGCCACCTG

     REVERSE PRIMER     187   20  60.08  50.00  AAGTCGGATTGGTCTGATGC




# EPRIMER3 RESULTS FOR seq_137

#                      Start  Len   Tm     GC%   Sequence





# EPRIMER3 RESULTS FOR seq_138

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 205
     FORWARD PRIMER      65   19  60.17  52.63  CATCGATTGATGGGCTGAG

     REVERSE PRIMER     249   21  59.88  52.38  CGGGAGAGCTGTGTTGAATAG


   2 PRODUCT SIZE: 205
     FORWARD PRIMER      65   19  60.17  52.63  CATCGATTGATGGGCTGAG

     REVERSE PRIMER     250   20  58.87  50.00  CGGGAGAGCTGTGTTGAATA




# EPRIMER3 RESULTS FOR seq_139

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER      19   20  59.94  50.00  TGCGACACGTCTTACACACA

     REVERSE PRIMER     199   20  59.60  50.00  GCGTGTGCTCCACACATATT


   2 PRODUCT SIZE: 205
     FORWARD PRIMER      14   20  60.33  55.00  CGTGATGCGACACGTCTTAC

     REVERSE PRIMER     199   20  59.60  50.00  GCGTGTGCTCCACACATATT




# EPRIMER3 RESULTS FOR seq_140

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 212
     FORWARD PRIMER      34   20  60.36  50.00  ATGAAGGAATGAGCCTGCTG

     REVERSE PRIMER     226   20  61.17  50.00  TGTGGAAAGCCTTGCGTATC


   2 PRODUCT SIZE: 212
     FORWARD PRIMER      34   20  60.36  50.00  ATGAAGGAATGAGCCTGCTG

     REVERSE PRIMER     227   19  59.29  47.37  TGTGGAAAGCCTTGCGTAT




# EPRIMER3 RESULTS FOR seq_141

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 208
     FORWARD PRIMER     530   20  60.40  55.00  TCGGCGTTCTGGTAGTTCTC

     REVERSE PRIMER     718   20  60.11  55.00  ACGATCTGGAGGTCTGGTTG


   2 PRODUCT SIZE: 200
     FORWARD PRIMER      34   20  60.59  55.00  GGGTTTAGGTCGGGTGAAAC

     REVERSE PRIMER     214   20  59.67  55.00  ATAGCCTCCGGTTCTTCTCC




# EPRIMER3 RESULTS FOR seq_142

#                      Start  Len   Tm     GC%   Sequence





# EPRIMER3 RESULTS FOR seq_143

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 202
     FORWARD PRIMER      17   20  60.87  50.00  GGACATCCCATTCGAGTTCA

     REVERSE PRIMER     199   20  60.06  55.00  CAGCGACGCTAGAGCTTCTT


   2 PRODUCT SIZE: 226
     FORWARD PRIMER     228   20  59.98  60.00  GCCTTAGCTCTCCCTCAGGT

     REVERSE PRIMER     434   20  59.97  50.00  AACTCGGAACCACGATCAAC




# EPRIMER3 RESULTS FOR seq_144

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 205
     FORWARD PRIMER     135   20  60.40  55.00  GAAGGGCGTCTCGTGAACTA

     REVERSE PRIMER     320   20  59.26  55.00  CCACGATCACCAGAGACTTG


   2 PRODUCT SIZE: 205
     FORWARD PRIMER     275   19  60.11  57.90  GTCGATGGACGTCGTGTTC

     REVERSE PRIMER     460   20  59.77  50.00  ACAGAACTCCTTGCCGTTGT




# EPRIMER3 RESULTS FOR seq_145

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 220
     FORWARD PRIMER      57   20  59.96  50.00  CCGAACCAGAATGCCTATGT

     REVERSE PRIMER     257   20  59.93  50.00  TTTAGAGTCCGGGGTTGATG


   2 PRODUCT SIZE: 209
     FORWARD PRIMER      68   20  59.23  45.00  TGCCTATGTCGAATCCTTCA

     REVERSE PRIMER     257   20  59.93  50.00  TTTAGAGTCCGGGGTTGATG




# EPRIMER3 RESULTS FOR seq_146

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER      13   20  59.50  45.00  GCGAAAACTGCCAGAAACTT

     REVERSE PRIMER     193   20  60.41  60.00  CCCCTTCAGGTACCAGTGTG


   2 PRODUCT SIZE: 202
     FORWARD PRIMER      13   20  59.50  45.00  GCGAAAACTGCCAGAAACTT

     REVERSE PRIMER     195   20  58.91  55.00  AACCCCTTCAGGTACCAGTG




# EPRIMER3 RESULTS FOR seq_147

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 200
     FORWARD PRIMER      91   20  60.07  55.00  CTCCACCGAGACCAGATCAT

     REVERSE PRIMER     272   19  61.24  63.16  CAGTACGTGGTGCCGTGAC


   2 PRODUCT SIZE: 200
     FORWARD PRIMER      91   20  60.07  55.00  CTCCACCGAGACCAGATCAT

     REVERSE PRIMER     273   18  60.32  61.11  CAGTACGTGGTGCCGTGA




# EPRIMER3 RESULTS FOR seq_148

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 203
     FORWARD PRIMER      79   20  59.81  50.00  AACCACCATATCCAGCAAGG

     REVERSE PRIMER     263   19  61.27  57.90  CATGTGACCGCCTGCTATG


   2 PRODUCT SIZE: 200
     FORWARD PRIMER      82   20  59.52  50.00  CACCATATCCAGCAAGGTGA

     REVERSE PRIMER     263   19  61.27  57.90  CATGTGACCGCCTGCTATG