view prinseq-graphs-noPCA.pl @ 0:9790cfb46d03 draft default tip

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#!/usr/bin/perl

#===============================================================================
#   Author: Robert SCHMIEDER, Computational Science Research Center @ SDSU, CA
#
#   File: prinseq-graphs
#   Date: 2012-12-22
#   Version: 0.6 graphs
#
#   Usage:
#      prinseq-graphs [options]
#
#      Try 'prinseq-graphs-noPCA -h' for more information.
#
#    Purpose: PRINSEQ will help you to preprocess your genomic or metagenomic
#             sequence data in FASTA or FASTQ format. The graphs version allows
#             users of the lite version to generate graphs similar to the web
#             version.
#
#    Bugs: Please use http://sourceforge.net/tracker/?group_id=315449
#
#===============================================================================

use strict;
use warnings;

use Getopt::Long;
use Pod::Usage;
use File::Temp qw(tempfile); #for output files
use Fcntl qw(:flock SEEK_END); #for log file
use Cwd;
use JSON;
use Cairo;
#use Statistics::PCA;
use MIME::Base64;
use File::Basename;
use Data::Dumper; ###

$| = 1; # Do not buffer output

my $PI = 4 * atan2(1, 1);
my $LOG62 = log(62);
my $DINUCODDS_VIR = [
                        [qw(1.086940308	0.98976932	1.034167044	0.880024041	1.070421277	0.990687084	0.890945575	1.069957074	0.92465631	0.803973303)],
                        [qw(1.101064857	0.986812783	1.038299155	0.896162618	1.081652847	0.976365237	0.867445186	1.06727283	0.94688543	0.768007295)],
                        [qw(1.071548411	0.912204166	1.196914981	0.80628184	1.294201511	1.148517794	0.269295791	1.033948026	0.895951033	0.623192149)],
                        [qw(1.090253719	0.907428629	1.203991784	0.786359294	1.281499107	1.145421568	0.235974709	1.033437274	0.899580091	0.631699771)],
                        [qw(1.075864745	1.003413074	1.01872902	0.897841689	0.980373171	1.05854979	0.934262259	1.052477953	0.88145851	0.889239724)],
                        [qw(1.101890467	1.030028291	1.019912674	0.84191395	1.0015174	1.069546264	0.900151602	0.996269395	0.889195343	0.904039022)],
                        [qw(1.152417359	0.855028574	0.91164793	1.017415486	1.114163672	1.128353311	0.846355573	0.916745489	1.206820475	0.811014651)],
                        [qw(1.142454218	0.8635465	0.923406967	1.026242747	1.134445058	1.131747833	0.79793368	0.920767641	1.179468556	0.799770057)],
                        [qw(1.124462747	0.873556143	0.945627041	1.013755408	1.159866153	1.096259526	0.757315047	0.972924919	1.105562567	0.772731886)],
                        [qw(1.143826972	0.866968779	0.995740249	0.945859278	1.109590621	1.089305083	0.76048874	0.971561388	1.157101408	0.792923027)],
                        [qw(1.131900141	0.82776996	0.996204924	0.999433455	1.024692372	1.071176333	0.921026216	1.088936699	1.054010776	0.773498892)],
                        [qw(1.042180476	0.930180412	1.019242897	0.98909997	1.006666828	1.046708539	0.959492164	1.011183418	1.055168776	0.937433818)],
                        [qw(1.086515695	0.985345815	0.930914307	0.969581792	1.043010232	1.087463712	0.939482285	0.990551965	0.954752469	0.893972874)],
                        [qw(1.096657826	0.950117614	0.936195529	0.965619788	1.114975275	1.077011195	0.843153131	0.989128406	1.043790912	0.840634731)],
                        [qw(1.158030995	0.935307365	0.874812261	1.056236525	1.117171274	0.937484692	1.057442372	0.970079538	1.174848738	0.725071711)],
                        [qw(1.15591506	0.93000227	0.883538923	1.0567652	1.095730954	0.944489906	1.074229471	0.983993745	1.156051409	0.726688465)],
                        [qw(1.205726473	0.924439339	1.049457756	0.805718412	0.975472778	1.07581991	0.726992211	1.075025787	0.8704929	0.726672843)],
                        [qw(1.188544681	0.95239611	1.049066985	0.790031334	1.038632598	1.056749787	0.665197397	1.057566244	0.862429061	0.708982398)],
                        [qw(1.063631482	0.925593715	1.014869316	0.944904401	1.119690731	1.325971834	0.273781451	0.943347677	1.06438014	0.920825904)],
                        [qw(1.077560287	0.911888545	1.044147857	0.927758054	1.058535939	1.296838544	0.421514996	0.945722451	1.128317986	0.926419928)],
                        [qw(1.163753415	0.989905668	0.893599328	0.955641844	1.176047687	0.941559156	0.950641089	0.959741692	1.100815282	0.72491925)],
                        [qw(1.139253929	0.946297517	0.922096125	1.024801537	1.205206793	0.968818717	0.915801342	0.971626058	1.107569276	0.627623404)]
                     ];
my $DINUCODDS_MIC = [
                        [qw(1.13127323	0.853587195	0.911041047	1.104520778	1.065586428	1.021434164	0.999734139	1.063684014	1.078035184	0.733596552)],
                        [qw(1.173267344	0.840539337	0.919534602	1.068050141	1.062394214	1.051999071	0.96770576	1.035511729	1.095600433	0.72328141)],
                        [qw(1.172939786	0.84567902	0.911836259	1.106288994	1.05351787	1.026143368	1.002308358	1.066319771	1.094918797	0.710733535)],
                        [qw(1.073527689	0.850290918	0.978455025	1.080882178	1.111174765	1.010754115	0.895668707	1.072980666	1.079304608	0.754057386)],
                        [qw(1.08807747	0.837444678	0.95824965	1.097310298	1.118897971	1.030863881	0.886827263	1.072349394	1.07406322	0.733440096)],
                        [qw(1.071685485	0.861055813	0.966566865	1.090268118	1.112945761	1.012538936	0.909535491	1.063745603	1.071156598	0.755770377)],
                        [qw(1.142698587	0.867936867	1.000612099	0.977934257	1.111801746	1.018318601	0.788556794	0.987763594	1.184649653	0.784776176)],
                        [qw(1.134560074	0.876651844	0.998190253	0.995723123	1.128448077	1.014172324	0.781776188	0.971020602	1.182411449	0.786449476)],
                        [qw(1.180029632	0.787899325	1.01316945	0.932268406	1.077837263	1.211699678	0.612128817	1.033036699	1.157314398	0.74940288)],
                        [qw(1.160925546	0.788308899	1.003702496	0.965371236	1.076051693	1.188304271	0.641536444	1.070331188	1.124067192	0.740126813)],
                        [qw(1.173873006	0.790118011	1.014718833	0.937979878	1.07453725	1.207167373	0.622279064	1.046150047	1.145627707	0.742212886)],
                        [qw(1.128383111	0.870541389	0.987269741	0.98353238	1.115643879	1.040107028	0.774505865	1.010896432	1.164757274	0.775254395)],
                        [qw(1.15297511	0.853883985	0.956393231	1.000027661	1.139915472	1.01355294	0.838843622	1.015553125	1.216219741	0.70447264)],
                        [qw(1.148264236	0.852123859	0.974568293	0.985455546	1.13192373	1.015879393	0.828987111	1.016820786	1.216647853	0.71634006)],
                        [qw(1.12933788	0.831777975	1.005434367	0.991081409	1.126146895	1.07421504	0.69343913	1.054032466	1.14809591	0.728541157)],
                        [qw(1.124157235	0.828112691	1.022348424	0.983822386	1.143028487	1.081830005	0.672594435	1.05685982	1.149537403	0.684432106)],
                        [qw(1.128029586	0.841853305	1.00983936	0.967179139	1.122524003	1.094555807	0.659238308	1.061578854	1.1243601	0.740148171)],
                        [qw(1.093521636	0.855071052	0.929160818	1.203773691	1.178257185	0.881341255	1.078305505	1.051988532	1.169143967	0.555057308)],
                        [qw(1.073737278	0.877396537	0.968017446	1.124155374	1.166244435	0.909044208	0.999147578	1.071098934	1.120156138	0.607444953)],
                        [qw(1.092150184	0.863407008	0.927040387	1.185387013	1.171670826	0.882276859	1.083058605	1.048379554	1.168635365	0.580337997)]
                    ];
my $DATA_VIR = [
                    [2,1,'Human (fecal)',[127/255, 127/255, 255/255,1]],
                    [3,1,'Human (fecal)',[127/255, 127/255, 255/255,1]],
                    [42,2,'Human (nasal)',[127/255, 127/255, 255/255,1]],
                    [43,2,'Human (nasal)',[127/255, 127/255, 255/255,1]],
                    [45,1,'Human (fecal)',[127/255, 127/255, 255/255,1]],
                    [49,1,'Human (fecal)',[127/255, 127/255, 255/255,1]],
                    [52,3,'Human (sputum)',[127/255, 127/255, 255/255,1]],
                    [54,3,'Human (sputum)',[127/255, 127/255, 255/255,1]],
                    [55,4,'Human (sputum, CF)',[127/255, 127/255, 255/255,1]],
                    [57,4,'Human (sputum, CF)',[127/255, 127/255, 255/255,1]],
                    [88,5,'Freshwater (Hot spring)',[127/255, 127/255, 255/255,1]],
                    [89,5,'Freshwater (Hot spring)',[127/255, 127/255, 255/255,1]],
                    [98,6,'Freshwater (Antartic lake)',[127/255, 127/255, 255/255,1]],
                    [99,6,'Freshwater (Antartic lake)',[127/255, 127/255, 255/255,1]],
                    [100,7,'Freshwater (reclaimed)',[127/255, 127/255, 255/255,1]],
                    [102,7,'Freshwater (reclaimed)',[127/255, 127/255, 255/255,1]],
                    [153,8,'Mouse (brain tissue)',[127/255, 127/255, 255/255,1]],
                    [154,8,'Mouse (brain tissue)',[127/255, 127/255, 255/255,1]],
                    [202,9,'Fish (gut)',[127/255, 127/255, 255/255,1]],
                    [206,9,'Fish (gut)',[127/255, 127/255, 255/255,1]],
                    [209,10,'Mosquito',[127/255, 127/255, 255/255,1]],
                    [211,10,'Mosquito',[127/255, 127/255, 255/255,1]],
		    ['U',0,'User input',[255/255, 127/255, 127/255,1]]
                ];
my $DATA_MIC = [
                    [17,1,'Human (fecal)',[127/255, 127/255, 255/255,1]],
                    [20,1,'Human (fecal)',[127/255, 127/255, 255/255,1]],
                    [22,1,'Human (fecal)',[127/255, 127/255, 255/255,1]],
                    [63,2,'Mouse (fecal)',[127/255, 127/255, 255/255,1]],
                    [65,2,'Mouse (fecal)',[127/255, 127/255, 255/255,1]],
                    [68,2,'Mouse (fecal)',[127/255, 127/255, 255/255,1]],
                    [93,3,'Marine (coastal)',[127/255, 127/255, 255/255,1]],
                    [95,3,'Marine (coastal)',[127/255, 127/255, 255/255,1]],
                    [109,4,'Marine (open ocean)',[127/255, 127/255, 255/255,1]],
                    [110,4,'Marine (open ocean)',[127/255, 127/255, 255/255,1]],
                    [111,4,'Marine (open ocean)',[127/255, 127/255, 255/255,1]],
                    [120,3,'Marine (coastal)',[127/255, 127/255, 255/255,1]],
                    [124,5,'Marine (estuary)',[127/255, 127/255, 255/255,1]],
                    [125,5,'Marine (estuary)',[127/255, 127/255, 255/255,1]],
                    [134,3,'Marine (coastal)',[127/255, 127/255, 255/255,1]],
                    [146,3,'Marine (coastal)',[127/255, 127/255, 255/255,1]],
                    [148,3,'Marine (coastal)',[127/255, 127/255, 255/255,1]],
                    [201,6,'Fish (gut)',[127/255, 127/255, 255/255,1]],
                    [203,7,'Fish (slime)',[127/255, 127/255, 255/255,1]],
                    [205,6,'Fish (gut)',[127/255, 127/255, 255/255,1]],
		    ['U',0,'User input',[255/255, 127/255, 127/255,1]]
                ];
my $BASE64_BASES = {A => 'iVBORw0KGgoAAAANSUhEUgAAAEkAAABJCAYAAABxcwvcAAAAGXRFWHRTb2Z0d2FyZQBBZG9iZSBJ
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		    G => 'iVBORw0KGgoAAAANSUhEUgAAAEkAAABJCAYAAABxcwvcAAAAGXRFWHRTb2Z0d2FyZQBBZG9iZSBJ
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		    T => 'iVBORw0KGgoAAAANSUhEUgAAAEkAAABJCAYAAABxcwvcAAAAGXRFWHRTb2Z0d2FyZQBBZG9iZSBJ
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SUVORK5CYII='
		    };
my $MMCHART_B2 = 'iVBORw0KGgoAAAANSUhEUgAAAAEAAAAGCAYAAAACEPQxAAAAGXRFWHRTb2Z0d2FyZQBBZG9iZSBJ
bWFnZVJlYWR5ccllPAAAABdJREFUeNpiYGBg+M/w//9/BmwEQIABANxBD/HRDNRSAAAAAElFTkSu
QmCC';
my $FREQCHART_L = 'iVBORw0KGgoAAAANSUhEUgAAAC8AAABvCAIAAADzHQ6XAAAAGXRFWHRTb2Z0d2FyZQBBZG9iZSBJ
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tXHhEu7cYqX++t/dbY83vyOr2WnHEu78rwADABaBbeIZChwYAAAAAElFTkSuQmCC';

my $CSS_STYLE = '
html, body, div, span, p, img {
    margin: 0;
    padding: 0;
    border: 0;
    outline: 0;
    font-size: 100%;
    vertical-align: baseline;
    background: transparent;
}

html, body {
    font-family: Arial, Verdana;
    color: #40454b;
    font-size: 12px;
    text-align: center;
}

img {
    padding: 0px; margin: 0px; border: none;
}

.info-panel {
    margin-top: 10px;
    margin-bottom: 10px;
    width: 740px;
    text-align: left;
}

.info-header {
    padding-top: 20px;
    padding-top: 10px;
}

.info-header-title {
    color: #126499;
    text-decoration: none;
    font-family: sans-serif;
    font-weight: bold;
    font-size: 16px;
    vertical-align: baseline;
    margin-right: 20px;
    margin-bottom: 25px;
    margin-top: 15px;
}

.info-content {
    padding: 2px;
    font-family: "lucida grande",sans-serif,arial;
    margin-top: 15px;
    margin-bottom: 15px;
}

.info-table-type {
    min-width: 70px;
    padding: 4px;
    vertical-align: top;
}

.info-table-value {
    font-weight: bold;
    padding-top: 4px;
    padding-left: 10px;
    padding-right: 10px;
    vertical-align: top;
}

hr {
    background-color: #E0E0E0;
    border: medium none;
    color: #E0E0E0;
    height: 1px;
    outline: medium none;
}

.sequencetext {
    font-family: courier, "courier new";
    font-weight: normal;
}
';

my $VERSION = '0.6';
my $WHAT = 'graphs-noPCA';

my $man = 0;
my $help = 0;
my %params = ('help' => \$help, 'h' => \$help, 'man' => \$man);
GetOptions( \%params,
            'help|h',
            'man',
            'verbose',
            'version' => sub { print "PRINSEQ-$WHAT $VERSION\n"; exit; },
            'i=s',
	    'o=s',
	    'png_all',
	    'html_all',
            'log:s',
            'web:s'
            ) or pod2usage(2);
pod2usage(1) if $help;
pod2usage(-exitstatus => 0, -verbose => 2) if $man;

=head1 NAME

PRINSEQ - PReprocessing and INformation of SEQuence data

=head1 VERSION

PRINSEQ-graphs 0.6

=head1 SYNOPSIS

perl prinseq-graphs.pl [-h] [-help] [-version] [-man] [-verbose] [-i input_graph_data_file] [-png_all] [-html_all] [-log file]

=head1 DESCRIPTION

PRINSEQ will help you to preprocess your genomic or metagenomic sequence data in FASTA (and QUAL) or FASTQ format. The graphs version allows users of the lite version to generate graphs similar to the web version.

=head1 OPTIONS

=over 8

=item B<-help> | B<-h>

Print the help message; ignore other arguments.

=item B<-man>

Print the full documentation; ignore other arguments.

=item B<-version>

Print program version; ignore other arguments.

=item B<-verbose>

Prints status and info messages during processing.

=item B<***** INPUT OPTIONS *****>

=item B<-i> <file>

Input file containing the graph data generated by the lite version.

=item B<***** OUTPUT OPTIONS *****>

=item B<-o> <string>

By default, the output files are created in the same directory as the input file with an additional "_prinseq_graphs_XXXX" in their name (where XXXX is replaced by random characters to prevent overwriting previous files). To change the output filename and location, specify the filename using this option. The file extension will be added automatically.

=item B<-png_all>

Use this option to generate PNG files with the graphs.

=item B<-html_all>

Use this option to generate a HTML file with the graphs and tables.

=item B<-log> <file>

Log file to keep track of parameters, errors, etc. The log file name is optional. If no file name is given, the log file name will be "inputname.log". If the log file already exists, new content will be added to the file.

=back

=head1 AUTHOR

Robert SCHMIEDER, C<< <rschmieder_at_gmail_dot_com> >>

=head1 BUGS

If you find a bug please email me at C<< <rschmieder_at_gmail_dot_com> >> or use http://sourceforge.net/tracker/?group_id=315449 so that I can make PRINSEQ better.

=head1 COPYRIGHT

Copyright (C) 2011-2012  Robert SCHMIEDER

=head1 LICENSE

This program is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the GNU General Public License for more details.

You should have received a copy of the GNU General Public License along with this program.  If not, see <http://www.gnu.org/licenses/>.

=cut

#
################################################################################
## DATA AND PARAMETER CHECKING
################################################################################
#

my ($file1,$command,@dataread);

#Check if input file exists and check if file format is correct
if(exists $params{i}) {
    $command .= ' -i '.$params{i};
    $file1 = $params{i};
    if($params{i} eq 'stdin') {
        my $format = &checkInputFormat();
        unless($format eq 'gd') {
            &printError('input data for -i is in '.uc($format).' format not in graph data format');
        }
    } elsif(-e $params{i}) {
        #check for file format
        my $format = &checkFileFormat($file1);
        unless($format eq 'gd') {
            &printError('input file for -i is in '.uc($format).' format not in graph data format');
        }
    } else {
        &printError("could not find input file \"".$params{i}."\"");
    }
} else {
    &printError("you did not specify an input file containing the graph data");
}

#check output file name prefix
if(exists $params{o}) {
    $command .= ' -o '.$params{o};
}

#check for output format
unless(exists $params{png_all} || exists $params{html_all}) {
    &printError("No output format specified. Use -png_all and/or -html_all to generate graphs.");
}
if(exists $params{png_all}) {
    $command .= ' -png_all';
}
if(exists $params{html_all}) {
    $command .= ' -html_all';
}
if(exists $params{web}) {
    $command .= ' -web'.($params{web} ? ' '.$params{web} : '');
}

#add remaining to log command
if(exists $params{log}) {
    $command .= ' -log'.($params{log} ? ' '.$params{log} : '');

    unless($params{log}) {
	$params{log} = join("__",$file1||'nonamegiven').'.log';
    }
    $params{log} = cwd().'/'.$params{log} unless($params{log} =~ /^\//);
    &printLog("Executing PRINSEQ with command: \"perl prinseq-".$WHAT.".pl".$command."\"");
}

#
################################################################################
## DATA PROCESSING
################################################################################
#

my $filename = $file1;
while($filename =~ /[\w\d]+\.[\w\d]+$/) {
    $filename =~ s/\.[\w\d]+$//;
    last if($filename =~ /\/[^\.]+$/);
}

if(exists $params{png_all}) {
    my $graphs = &generateGraphs($params{i},$params{o});
    if(exists $params{web} && $params{web} ne 'nozip') {
        #png files
        if(scalar(@$graphs)) {
            system("zip -j -r ".dirname($params{o})."/png_graphs.zip ".dirname($params{o}).' -i \*.png') == 0 or &printError("Cannot generate graphs ZIP file");
        }
    }
}
if(exists $params{html_all}) {
    &generateHtml($params{i},$params{o});
}

&printWeb("STATUS: done");

##
#################################################################################
### MISC FUNCTIONS
#################################################################################
##

sub printError {
    my $msg = shift;
    print STDERR "\nERROR: ".$msg.".\n\nTry \'perl prinseq-".$WHAT.".pl -h\' for more information.\nExit program.\n";
    &printLog("ERROR: ".$msg.". Exit program.\n");
    exit(0);
}

sub printWarning {
    my $msg = shift;
    print STDERR "WARNING: ".$msg.".\n";
    &printLog("WARNING: ".$msg.".\n");
}

sub printWeb {
    my $msg = shift;
    if(exists $params{web}) {
        print STDERR "\n".&getTime()."$msg\n";
    }
}

sub getTime {
    return sprintf("[%02d/%02d/%04d %02d:%02d:%02d] ",sub {($_[4]+1,$_[3],$_[5]+1900,$_[2],$_[1],$_[0])}->(localtime));
}

sub printLog {
    my $msg = shift;
    if(exists $params{log}) {
        my $time = sprintf("%02d/%02d/%04d %02d:%02d:%02d",sub {($_[4]+1,$_[3],$_[5]+1900,$_[2],$_[1],$_[0])}->(localtime));
        open(FH, ">>", $params{log}) or die "ERROR: Can't open file ".$params{log}.": $! \n";
        flock(FH, LOCK_EX) or die "ERROR: Cannot lock file ".$params{log}.": $! \n";
        print FH "[prinseq-".$WHAT."-$VERSION] [$time] $msg\n";
        flock(FH, LOCK_UN) or die "ERROR: cannot unlock ".$params{log}.": $! \n";
        close(FH);
    }
}

sub addCommas {
    my $num = shift;
    return unless(defined $num);
    return $num if($num < 1000);
    $num = scalar reverse $num;
    $num =~ s/(\d{3})/$1\,/g;
    $num =~ s/\,$//;
    $num = scalar reverse $num;
    return $num;
}

sub checkFileFormat {
    my $file = shift;

    my ($format,$count,$id,$fasta,$fastq,$qual,$gd,$aa);
    $count = 3;
    $fasta = $fastq = $qual = $gd = $aa = 0;
    $format = 'unknown';

    open(FILE,"perl -p -e 's/\r/\n/g;s/\n\n/\n/g' < $file |") or die "ERROR: Could not open file $file: $! \n";
    while (<FILE>) {
#        chomp();
 #       next unless(length($_));
        if($count-- == 0) {
            last;
        } elsif(!$fasta && /^\>\S+\s*/) {
            $fasta = 1;
            $qual = 1;
        } elsif($fasta == 1 && (($aa && /^[ABCDEFGHIKLMNOPQRSTUVWYZXabcdefghiklmmopqrstuvwyzx*-]+/) || (!$aa && /^[ACGTURYKMSWBDHVNXacgturykmswbdhvnx-]+/))) {
            $fasta = 2;
        } elsif($qual == 1 && /^\s*\d+/) {
            $qual = 2;
        } elsif(!$fastq && /^\@(\S+)\s*/) {
            $id = $1;
            $fastq = 1;
        } elsif($fastq == 1 && (($aa && /^[ABCDEFGHIKLMNOPQRSTUVWYZXabcdefghiklmmopqrstuvwyzx*-]+/) || (!$aa && /^[ACGTURYKMSWBDHVNXacgturykmswbdhvnx-]+/))) {
            $fastq = 2;
        } elsif($fastq == 2 && /^\+(\S*)\s*/) {
            $fastq = 3 if($id eq $1 || /^\+\s*$/);
        } elsif(!$gd && /^\{\"numseqs\"\:/) {
	    $gd = 1;
	}
    }
    close(FILE);
    if($fasta == 2) {
        $format = 'fasta';
    } elsif($qual == 2) {
        $format = 'qual';
    } elsif($fastq == 3) {
        $format = 'fastq';
    } elsif($gd == 1) {
	$format = 'gd';
    }

    return $format;
}

sub checkInputFormat {
    my ($format,$count,$id,$fasta,$fastq,$qual,$gd,$aa);
    $count = 3;
    $fasta = $fastq = $qual = $gd = $aa = 0;
    $format = 'unknown';

    while (<STDIN>) {
        push(@dataread,$_);
#        chomp();
 #       next unless(length($_));
        if($count-- == 0) {
            last;
        } elsif(!$fasta && /^\>\S+\s*/) {
            $fasta = 1;
            $qual = 1;
        } elsif($fasta == 1 && (($aa && /^[ABCDEFGHIKLMNOPQRSTUVWYZXabcdefghiklmmopqrstuvwyzx*-]+/) || (!$aa && /^[ACGTURYKMSWBDHVNXacgturykmswbdhvnx-]+/))) {
            $fasta = 2;
        } elsif($qual == 1 && /^\s*\d+/) {
            $qual = 2;
        } elsif(!$fastq && /^\@(\S+)\s*/) {
            $id = $1;
            $fastq = 1;
        } elsif($fastq == 1 && (($aa && /^[ABCDEFGHIKLMNOPQRSTUVWYZXabcdefghiklmmopqrstuvwyzx*-]+/) || (!$aa && /^[ACGTURYKMSWBDHVNXacgturykmswbdhvnx-]+/))) {
            $fastq = 2;
        } elsif($fastq == 2 && /^\+(\S*)\s*/) {
            $fastq = 3 if($id eq $1 || /^\+\s*$/);
        } elsif(!$gd && /^\{\"numseqs\"\:/) {
	    $gd = 1;
	}
    }

    if($fasta == 2) {
        $format = 'fasta';
    } elsif($qual == 2) {
        $format = 'qual';
    } elsif($fastq == 3) {
        $format = 'fastq';
    } elsif($gd == 1) {
	$format = 'gd';
    }

    return $format;
}

sub readGdFile {
    my $file = shift;
    my $data;

    open(DATA,"<$file") or &printError("Could not open file $file: $!");
    while(<DATA>) {
	next if(/^\#/);
        chomp();
	if(length($_)) {
	    $data = from_json($_);
	}
    }
    close(DATA);

    return $data;
}

sub getFileName {
    my $ext = shift;
    my ($file,$fh);
    if(exists $params{o}) {
	$file = $params{o}.$ext;
	open(OUT,">$file") or &printError('cannot open output file');
	close(OUT);
    } else {
	$fh = File::Temp->new( TEMPLATE => $filename.'_prinseq_graphs_XXXX',
			       SUFFIX => $ext,
			       UNLINK => 0);
	$file = $fh->filename;
	$fh->close();
    }
    return $file;
}

sub generateGraphs {
    my ($in,$out) = @_;
    my ($file,$data,$surface,@graphs);
    $data = &readGdFile($in);

    #length plot
    if(exists $data->{counts}->{length}) {
	$file = &getFileName('_ld.png');
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts}->{length},1),$data->{stats}->{length},'Length Distribution','Read Length in bp','# Sequences',$file,0,' bp');
	$surface->write_to_png($file);
        push(@graphs,$file);
    }
    if(exists $data->{counts2} && exists $data->{counts2}->{length}) {
	$file = &getFileName('_ld-2.png');
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts2}->{length},1),$data->{stats2}->{length},'Length Distribution','Read Length in bp','# Sequences',$file,0,' bp');
	$surface->write_to_png($file);
        push(@graphs,$file);
    }

    #tail plot
    if(exists $data->{tail}) {
	$file = &getFileName('_td5.png');
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts}->{tail5},1),undef,'Poly-A/T Tail Distribution (> 4bp)','5\' Tail Length in bp','# Sequences',$file,0,' bp');
	$surface->write_to_png($file);
        push(@graphs,$file);
	$file = &getFileName('_td3.png');
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts}->{tail3},1),undef,'Poly-A/T Tail Distribution (> 4bp)','3\' Tail Length in bp','# Sequences',$file,0,' bp');
	$surface->write_to_png($file);
        push(@graphs,$file);
    }
    if(exists $data->{tail2}) {
	$file = &getFileName('_td5-2.png');
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts2}->{tail5},1),undef,'Poly-A/T Tail Distribution (> 4bp)','5\' Tail Length in bp','# Sequences',$file,0,' bp');
	$surface->write_to_png($file);
        push(@graphs,$file);
	$file = &getFileName('_td3-2.png');
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts2}->{tail3},1),undef,'Poly-A/T Tail Distribution (> 4bp)','3\' Tail Length in bp','# Sequences',$file,0,' bp');
	$surface->write_to_png($file);
        push(@graphs,$file);
    }

    #Ns plot
    if(exists $data->{counts}->{ns}) {
	$file = &getFileName('_ns.png');
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts}->{ns},1),undef,'Percentage of N\'s (> 0%)','Percentage of N\'s per Read (1-100%)','# Sequences',$file,0);
	$surface->write_to_png($file);
        push(@graphs,$file);
    }
    if(exists $data->{counts2} && exists $data->{counts2}->{ns}) {
	$file = &getFileName('_ns-2.png');
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts2}->{ns},1),undef,'Percentage of N\'s (> 0%)','Percentage of N\'s per Read (1-100%)','# Sequences',$file,0);
	$surface->write_to_png($file);
        push(@graphs,$file);
    }

    #GC content plot
    if(exists $data->{counts}->{gc}) {
	$file = &getFileName('_gc.png');
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts}->{gc},0),$data->{stats}->{gc},'GC Content Distribution','GC Content (0-100%)','Number of Sequences',$file,1);
	$surface->write_to_png($file);
        push(@graphs,$file);
    }
    if(exists $data->{counts2} && exists $data->{counts2}->{gc}) {
	$file = &getFileName('_gc-2.png');
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts2}->{gc},0),$data->{stats2}->{gc},'GC Content Distribution','GC Content (0-100%)','Number of Sequences',$file,1);
	$surface->write_to_png($file);
        push(@graphs,$file);
    }

    #Sequence complexity plot - dust
    if(exists $data->{compldust}) {
	$file = &getFileName('_cd.png');
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{compldust},0),undef,'Sequence complexity distribution','Mean sequence complexity (DUST scores)','Number of sequences',$file,1);
	$surface->write_to_png($file);
        push(@graphs,$file);
    }

    #Sequence complexity plot - entropy
    if(exists $data->{complentropy}) {
	$file = &getFileName('_ce.png');
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{complentropy},0),undef,'Sequence complexity distribution','Mean sequence complexity (Entropy values)','Number of sequences',$file,1);
	$surface->write_to_png($file);
        push(@graphs,$file);
    }

    #Dinucleotide odd ratio PCA plot - microbial/viral
    #Odds ratio plot
    if(exists $data->{dinucodds}) {
	my @new = map {$data->{dinucodds}->{$_}} sort keys %{$data->{dinucodds}};
#	$file = &getFileName('_pm.png');
#	$surface = &createPCAPlot(&convertToPCAValues(\@new,'m'),'PCA','1st Principal Component Score','2nd Principal Component Score',$file);
#	$surface->write_to_png($file);
#        push(@graphs,$file);
#	$file = &getFileName('_pv.png');
#	$surface = &createPCAPlot(&convertToPCAValues(\@new,'v'),'PCA','1st Principal Component Score','2nd Principal Component Score',$file);
#	$surface->write_to_png($file);
#        push(@graphs,$file);
	$file = &getFileName('_or.png');
	$surface = &createOddsRatioPlot($data->{dinucodds},'Odds ratios','Dinucleotide','Odds ratio',$file);
	$surface->write_to_png($file);
        push(@graphs,$file);
    }

    #Qual plot
    if(exists $data->{quals}) {
	$file = &getFileName('_qd.png');
	$surface = &createBoxPlot(&convertToBoxValues($data->{quals},4),'Base Quality Distribution','Read position in %','Quality score',$file);
	$surface->write_to_png($file);
        push(@graphs,$file);
    }
    if(exists $data->{quals2}) {
	$file = &getFileName('_qd-2.png');
	$surface = &createBoxPlot(&convertToBoxValues($data->{quals2},4),'Base Quality Distribution','Read position in %','Quality score',$file);
	$surface->write_to_png($file);
        push(@graphs,$file);
    }

    #Qualbin plot
    if(exists $data->{qualsbin}) {
	$file = &getFileName('_qd2.png');
	$surface = &createBoxPlot(&convertToBoxValues($data->{qualsbin},4),'Base Quality Distribution','Read position in bp','Quality score',$file,0,'bp',$data->{binval});
       $surface->write_to_png($file);
       push(@graphs,$file);
    }
    if(exists $data->{qualsbin2}) {
	$file = &getFileName('_qd2-2.png');
	$surface = &createBoxPlot(&convertToBoxValues($data->{qualsbin2},4),'Base Quality Distribution','Read position in bp','Quality score',$file,0,'bp',$data->{binval});
       $surface->write_to_png($file);
       push(@graphs,$file);
    }

    #Qualmean plot
    if(exists $data->{qualsmean}) {
	$file = &getFileName('_qd3.png');
	$surface = &createBarPlot(&convertToBarValues($data->{qualsmean},5,1),'Sequence Quality Distribution','Mean of quality scores per sequence','Number of sequences',$file,0);
	$surface->write_to_png($file);
        push(@graphs,$file);
    }
    if(exists $data->{qualsmean2}) {
	$file = &getFileName('_qd3-2.png');
	$surface = &createBarPlot(&convertToBarValues($data->{qualsmean2},5,1),'Sequence Quality Distribution','Mean of quality scores per sequence','Number of sequences',$file,0);
	$surface->write_to_png($file);
        push(@graphs,$file);
    }

    #Sequence duplicate plots
    if(exists $data->{dubscounts}) {
	$file = &getFileName('_df.png');
	$surface = &createStackBarPlot(&convertOdToStackBinMatrix($data->{dubscounts},5,1,100),'Sequence duplication level','Number of duplicates','Number of sequences',$file,0);
	$surface->write_to_png($file);
        push(@graphs,$file);
    }
    if(exists $data->{dubslength}) {
	$file = &getFileName('_dl.png');
	$surface = &createStackBarPlot(&convertOdToStackBinMatrix($data->{dubslength},5,1),'Sequence duplication level','Read Length in bp','Number of duplicates',$file,0,' bp');
	$surface->write_to_png($file);
        push(@graphs,$file);
    }
    if(exists $data->{dubscounts}) {
	my %dubsmax;
	my $count = 1;
	foreach my $n (sort {$b <=> $a} keys %{$data->{dubscounts}}) {
	    foreach my $s (keys %{$data->{dubscounts}->{$n}}) {
		foreach my $i (1..$data->{dubscounts}->{$n}->{$s}) {
		    $dubsmax{$count++}->{$s} = $n;
		    last unless($count <= 100);
		}
		last unless($count <= 100);
	    }
	    last unless($count <= 100);
	}
	$file = &getFileName('_dm.png');
	$surface = &createStackBarPlot(&convertOdToStackBinMatrix(\%dubsmax,5,1,100),'Sequence duplication level','Sequence','Number of duplicates',$file,0);
	$surface->write_to_png($file);
        push(@graphs,$file);
    }

    return \@graphs;
}

sub convertOdToBinMatrix {
    my ($data,$min,$max,$nonice) = @_;

    my ($num,$ymax,$xmax,$xmin,$step,%vals,$tmp,@matrix,$bin,$tmpbin);

    #make nice xmax value
    if(defined $max) {
	$xmax = $max;
    } else {
	$xmax = (sort {$b <=> $a} keys %$data)[0];
    }
    $bin = &getBinVal($xmax);
    $xmax = $bin*100;
    $xmin = (defined $min ? $min : 0);

    #get data to bin and find y axis max value
    $ymax = 0;
    $tmp = 0;
    $tmpbin = $bin;
    foreach my $i ($xmin..$xmax) {
	if(exists $data->{$i}) {
	    $tmp += $data->{$i};
	}
	if(--$tmpbin <= 0) {
	    $tmpbin = $bin;
	    $ymax = &max($ymax,$tmp);
	    push(@matrix,$tmp);
	    $tmp = 0;
	}
    }

    #make nice ymax value
    unless($nonice) {
	$ymax = sprintf("%d",($ymax/4)+1)*4 if($ymax % 4);
#        $step = ($ymax <= 10 ? 10 : ($ymax < 40 ? 40 : ($ymax < 100 ? 100 : ($ymax < 1000 ? 100 : 100))));
#        $ymax = sprintf("%d",($ymax/$step)+1)*$step if($ymax % $step);
    }

    return (\@matrix,$xmax,$ymax);
}

sub getBinVal {
    my $val = shift;
    my $step;
    if(!$val || $val <= 100) {
	return 1;
    } elsif($val < 10000) {
	return int($val/100)+($val % 100 ? 1 : 0);
    } elsif($val < 100000) {
	return 1000;
    } else {
	$step = 1000000;
	my $xmax = ($val % $step ? sprintf("%d",($val/$step+1))*$step : $val);
	return ($xmax/100);
    }
}

sub max {
    my ($a,$b) = @_;
    return ($a < $b ? $b : $a);
}

sub min {
    my ($a,$b) = @_;
    return ($a > $b ? $b : $a);
}

sub createAnnotBarPlot {
    my ($matrix,$xmax,$ymax,$annot,$title,$xlab,$ylab,$file,$zero,$add) = @_;

    my $bin = 1;
    if($xmax > 100) {
	$bin = $xmax / 100;
	$xmax = 100;
    }

    my @barcol = (127/255, 127/255, 255/255, 1); #b2b2ff
    my @meancol = (255/255, 127/255, 127/255, 1); #ffb2b2
    my @stdcol = (178/255, 178/255, 255/255, 0.8); #7f7fff
    my @std1col = (0, 0, 0, 0.04); #ff7f7f
    my @std2col = (0, 0, 0, 0.03); #ff7f7f
    my @linecol = (0, 0, 0, 0.4);
    my @helplinecol = (1, 1, 1, 0.9);
    my @background = (0.95, 0.95, 0.95, 1);
    my @tickcol = (0, 0, 0, 0.8);
    my @labelcol = (0, 0, 0, 1);

    #create new image
    my $size   = 6;
    my $offset = 20;
    my $left   = 40;
    my $bottom = 15;
    my $top = 20;
    my $height = 200;
    my $surface = Cairo::ImageSurface->create('argb32', $left+$offset*2+($xmax+$zero)*$size,$bottom+$top+$offset*2+$height); #format, width, height
    my $cr = Cairo::Context->create($surface);

    my ($font_extents,$extents,$fontheight,$fontdescent);

    #background
    $cr->rectangle(0, 0, $left+$offset*2+($xmax+$zero)*$size,$bottom+$offset*2+2*200+20);
    $cr->set_source_rgba(1, 1, 1, 1);
    $cr->fill;

    #fonts
    $cr->select_font_face ('sans', 'normal', 'normal');

    $cr->save;

    #set up work space
    $cr->set_antialias('none');
    $cr->set_line_width(1);

    #background for plot
    $cr->rectangle($left+$offset, $top+$offset, ($xmax+$zero)*$size-1, $height);
    $cr->set_source_rgba(@background);
    $cr->fill;

    #draw ticks
    #x-axis
    $cr->set_source_rgba(@tickcol);
    foreach my $i (($zero ? 0 : 1)..$xmax) {
	if(($i%5) == 0 && $i > 1 && $i < $xmax) {
	    $cr->move_to($left+$offset+int($size/2)+$size*$i-($zero ? 0 : $size)-1, $top+$offset+$height);
	    $cr->line_to($left+$offset+int($size/2)+$size*$i-($zero ? 0 : $size)-1, $top+$offset+$height+3);
	} else {
	    $cr->move_to($left+$offset+int($size/2)+$size*$i-($zero ? 0 : $size)-1, $top+$offset+$height);
	    $cr->line_to($left+$offset+int($size/2)+$size*$i-($zero ? 0 : $size)-1, $top+$offset+$height+1);
	}
    }
    $cr->stroke;

    #y-axis
    $cr->move_to($left+$offset, $top+$offset);
    $cr->line_to($left+$offset-3, $top+$offset);
    $cr->move_to($left+$offset, $top+$offset+$height-1);
    $cr->line_to($left+$offset-3, $top+$offset+$height-1);
    $cr->stroke;

    #helplines
    $cr->set_source_rgba(@helplinecol);
    foreach my $j (1..3) {
        $cr->move_to($left+$offset, $top+$offset+$height*$j/4-($j ? 1 : 0));
        $cr->line_to($left+$offset+($xmax+$zero)*$size, $top+$offset+$height*$j/4-($j ? 1 : 0));
    }
    $cr->stroke;

    $cr->set_antialias('default');

    #tick labels
    $cr->set_source_rgba(@tickcol);
    $cr->set_font_size(10);
    $font_extents = $cr->font_extents;
    $fontheight = $font_extents->{height};
    #x-axis
    foreach my $i (($zero ? 0 : 1)..$xmax) {
	if(($i%10) == 0 && $i > 1 && $i < $xmax) {
	    $extents = $cr->text_extents($i*$bin);
	    $cr->move_to($left+$offset+int($size/2+1)+$size*$i-($zero ? 0 : $size)-$extents->{width}/2-1-($i == 1 ? 1 : 0), $top+$offset+$height+$fontheight+2);
	    $cr->show_text($i*$bin);
	}
    }
    #y-axis
    $extents = $cr->text_extents(&addCommas($ymax));
    $cr->move_to($left+$offset-5-$extents->{width}, $top+$offset+$fontheight/2-2);
    $cr->show_text(&addCommas($ymax));
    $extents = $cr->text_extents(0);
    $cr->move_to($left+$offset-5-$extents->{width}, $top+$offset+$fontheight/2-2+$height);
    $cr->show_text(0);

    $cr->save;

    #labels
    $cr->set_font_size (14);
    $font_extents = $cr->font_extents;
    $fontheight = $font_extents->{height};

    #axis labels
    $cr->set_source_rgba(@labelcol);
    $extents = $cr->text_extents($xlab.($bin>1 ? ' (Bin size: '.$bin.($add ? $add.')' : '') : ''));
    $cr->move_to($left+$offset+($xmax+$zero)*$size/2-$extents->{width}/2, $top+$offset+$height+$fontheight+15);
    $cr->show_text($xlab.($bin>1 ? ' (Bin size: '.$bin.($add ? $add.')' : '') : ''));
    $cr->rotate($PI * 3 / 2);
    $extents = $cr->text_extents($ylab.($bin>1 ? ' (per bin)' : ''));
    $cr->move_to(-($top+$offset+$height/2+$extents->{width}/2),$offset+10);
    $cr->show_text($ylab.($bin>1 ? ' (per bin)' : ''));

    $cr->restore;

    #draw annotations
    if($annot) {
	$cr->set_antialias('none');
	my ($std1l,$std2l,$std1r,$std2r);
	#std boxes
	$std1l = int($annot->{mean})-int($annot->{std});
	$std2l = int($annot->{mean})-2*int($annot->{std});
	$std1r = int($annot->{mean})+int($annot->{std});
	$std2r = int($annot->{mean})+2*int($annot->{std});
        unless($std1l == $std1r) {
            if($std1l < 0) {
                $std1l = 0;
            } else {
                $std1l = int($std1l/$bin);
            }
            if($std2l < 0) {
                $std2l = 0;
            } else {
                $std2l = int($std2l/$bin);
            }
            if($std1r/$bin > 100) {
                $std1r = 100;
            } else {
                $std1r = int($std1r/$bin);
            }
            if($std2r/$bin > 100) {
                $std2r = 100;
            } else {
                $std2r = int($std2r/$bin);
            }
            $cr->rectangle($left+$offset+$std2l*$size+2, $top+$offset, ($std2r-$std2l)*$size, $height);
            $cr->set_source_rgba(@std2col);
            $cr->fill;
            $cr->rectangle($left+$offset+$std1l*$size+2, $top+$offset, ($std1r-$std1l)*$size, $height);
            $cr->set_source_rgba(@std1col);
            $cr->fill;
            #mean line
            $cr->set_source_rgba(@meancol);
            $cr->move_to($left+$offset+int(int($annot->{mean})/$bin)*$size+2, $top+$offset-5);
            $cr->line_to($left+$offset+int(int($annot->{mean})/$bin)*$size+2, $top+$offset+$height);
            $cr->stroke;
            #std lines
            $cr->set_source_rgba(@stdcol);
            if($std1l > 0) {
                $cr->move_to($left+$offset+$std1l*$size+2, $top+$offset-5);
                $cr->line_to($left+$offset+$std1l*$size+2, $top+$offset+$height);
            }
            if($std2l > 0) {
                $cr->move_to($left+$offset+$std2l*$size+2, $top+$offset-5);
                $cr->line_to($left+$offset+$std2l*$size+2, $top+$offset+$height);
            }
            if($std1r < 100) {
                $cr->move_to($left+$offset+$std1r*$size+2, $top+$offset-5);
                $cr->line_to($left+$offset+$std1r*$size+2, $top+$offset+$height);
            }
            if($std2r < 100) {
                $cr->move_to($left+$offset+$std2r*$size+2, $top+$offset-5);
                $cr->line_to($left+$offset+$std2r*$size+2, $top+$offset+$height);
            }
            $cr->stroke;
            #labels
            $cr->set_antialias('default');
            $cr->set_source_rgba(@tickcol);
            $extents = $cr->text_extents('M');
            $cr->move_to($left+$offset+int(int($annot->{mean})/$bin)*$size+2-$extents->{width}/2, $top+$offset-10);
            $cr->show_text('M');
            if($std1l > 0) {
                $extents = $cr->text_extents('1SD');
                $cr->move_to($left+$offset+$std1l*$size-$extents->{width}/2+2, $top+$offset-10);
                $cr->show_text('1SD');
            }
            if($std2l > 0) {
                $extents = $cr->text_extents('2SD');
                $cr->move_to($left+$offset+$std2l*$size-$extents->{width}/2+3, $top+$offset-10);
                $cr->show_text('2SD');
            }
            if($std1r < 100) {
                $extents = $cr->text_extents('1SD');
                $cr->move_to($left+$offset+$std1r*$size-$extents->{width}/2+2, $top+$offset-10);
                $cr->show_text('1SD');
            }
            if($std2r < 100) {
                $extents = $cr->text_extents('2SD');
                $cr->move_to($left+$offset+$std2r*$size-$extents->{width}/2+3, $top+$offset-10);
                $cr->show_text('2SD');
            }
        }
    }

    #draw boxes
    $cr->set_antialias('none');
    $cr->set_source_rgba(@barcol);
    foreach my $pos (0..$xmax-($zero ? 0 : 1)) {
        next unless($matrix->[$pos]);
        my $tmp = $matrix->[$pos] / $ymax;
	#unique
        if($tmp) {
            $cr->rectangle($left+$offset+$pos*$size, $top+$offset+$height, $size-1, -$tmp*$height);
            $cr->fill;
        }
    }

    #write image
    $cr->show_page;
    return $surface;
}

#sub convertToPCAValues {
#    my ($new,$type) = @_;
#
#    my @data = ($type eq 'v' ? @$DINUCODDS_VIR : @$DINUCODDS_MIC);
#
#    push(@data,$new);
#
#    my $pca = Statistics::PCA->new;
#    $pca->load_data({format => 'table', data => \@data});
#    $pca->pca();
#
#    my @variances = $pca->results('proportion');
#    my @list = $pca->results('transformed');
#
#    my ($xmin,$xmax,$ymin,$ymax);
#    $xmax = $ymax = -100;
#    $xmin = $ymin = 100;
#
#    #get min/max values for PC1
#    foreach my $v (@{$list[0]}) {
#        $xmax = &max($xmax,$v);
#        $xmin = &min($xmin,$v);
#    }
#    #get min/max values for PC2
#    foreach my $v (@{$list[1]}) {
#        $ymax = &max($ymax,$v);
#        $ymin = &min($ymin,$v);
#    }
#
#    return ([$list[0],$list[1]],sprintf("%d",$variances[0]*100),sprintf("%d",$variances[1]*100),$xmin,$xmax,$ymin,$ymax,$type);
#}

sub createPCAPlot {
    my ($data,$var1,$var2,$xmin,$xmax,$ymin,$ymax,$type,$title,$xlab,$ylab,$file) = @_;

    my @linecol = (0, 0, 0, 0.4);
    my @helplinecol1 = (1,1,1, 0.9);
    my @helplinecol2 = (1,1,1, 0.5);

    #create new image
    my $size   = 5;
    my $offset = 20;
    my $left   = 25;
    my $bottom = 15;
    my $top = ($type eq 'v' ? 35 : 20);
    my $height = 500;
    my $space = 10;
    my $surface = Cairo::ImageSurface->create('argb32', $left+$offset*2+$height+2*$space,$top+$bottom+$offset*2+$height+2*$space); #format, width, height
    my $cr = Cairo::Context->create($surface);

    my ($font_extents,$extents,$fontheight,$fontdescent);

    #background
    $cr->rectangle(0, 0, $left+$offset*2+$height+2*$space,$top+$bottom+$offset*2+$height+2*$space);
    $cr->set_source_rgba(1, 1, 1, 1);
    $cr->fill;

    #fonts
    $cr->select_font_face ('sans-serif', 'normal', 'normal');

    $cr->save;

    #set up work space
    my ($dx, $dy);
    $cr->set_antialias('none');
    $cr->set_line_width(1);

    #background for plot
    $cr->rectangle($left+$offset, $top+$offset, $height+2*$space, $height+2*$space);
    $cr->set_source_rgba(0.95, 0.95, 0.95, 1);
    $cr->fill;

    #get infos
    my $num = scalar(@{$data->[0]})-1;
    my $xrange = ($xmax-$xmin);
    my $yrange = ($ymax-$ymin);
    my $data_info = ($type eq 'v' ? $DATA_VIR : $DATA_MIC);

    #draw ticks
    #x-axis
    $cr->set_source_rgba(0, 0, 0, 0.8);
    $cr->move_to($left+$offset+$space, $top+$offset+$height+2*$space);
    $cr->line_to($left+$offset+$space, $top+$offset+$height+2*$space+3);
    $cr->move_to($left+$offset+$space+$height, $top+$offset+$height+2*$space);
    $cr->line_to($left+$offset+$space+$height, $top+$offset+$height+2*$space+3);
    $cr->move_to($left+$offset+$space+int(abs($xmin)/$xrange*$height), $top+$offset+$height+2*$space);
    $cr->line_to($left+$offset+$space+int(abs($xmin)/$xrange*$height), $top+$offset+$height+2*$space+3);
    $cr->stroke;
    #y-axis
    $cr->move_to($left+$offset, $top+$offset+$space);
    $cr->line_to($left+$offset-3, $top+$offset+$space);
    $cr->move_to($left+$offset, $top+$offset+$height+$space);
    $cr->line_to($left+$offset-3, $top+$offset+$height+$space);
    $cr->move_to($left+$offset, $top+$offset+$space+int(abs($ymax)/$yrange*$height));
    $cr->line_to($left+$offset-3, $top+$offset+$space+int(abs($ymax)/$yrange*$height));
    $cr->stroke;

    #helplines
    $cr->set_source_rgba(@helplinecol1);
    $cr->move_to($left+$offset+$space+int(abs($xmin)/$xrange*$height), $top+$offset);
    $cr->line_to($left+$offset+$space+int(abs($xmin)/$xrange*$height), $top+$offset+$height+2*$space);
    $cr->stroke;
    $cr->move_to($left+$offset, $top+$offset+$space+int(abs($ymax)/$yrange*$height));
    $cr->line_to($left+$offset+2*$space+$height, $top+$offset+$space+int(abs($ymax)/$yrange*$height));
    $cr->stroke;


    $cr->set_antialias('default');

    #tick labels
    $cr->set_source_rgba(0, 0, 0, 0.8);
    $cr->set_font_size(10);
    $font_extents = $cr->font_extents;
    $fontheight = $font_extents->{height};
    #x-axis
    $extents = $cr->text_extents(sprintf("%.2f",$xmin));
    $cr->move_to($left+$offset+$space-$extents->{width}/2-1, $top+$offset+$height+2*$space+$fontheight+2);
    $cr->show_text(sprintf("%.2f",$xmin));
    $extents = $cr->text_extents(sprintf("%.2f",$xmax));
    $cr->move_to($left+$offset+$space+$height-$extents->{width}/2-1, $top+$offset+$height+2*$space+$fontheight+2);
    $cr->show_text(sprintf("%.2f",$xmax));
    $extents = $cr->text_extents(0);
    $cr->move_to($left+$offset+$space+int(abs($xmin)/$xrange*$height)-$extents->{width}/2, $top+$offset+$height+2*$space+$fontheight+2);
    $cr->show_text(0);
    #y-axis
    $extents = $cr->text_extents(sprintf("%.2f",$ymax));
    $cr->move_to($left+$offset-5-$extents->{width}, $top+$offset+$space+$fontheight/2-2);
    $cr->show_text(sprintf("%.2f",$ymax));
    $extents = $cr->text_extents(sprintf("%.2f",$ymin));
    $cr->move_to($left+$offset-5-$extents->{width}, $top+$offset+$height+$space+$fontheight/2-2);
    $cr->show_text(sprintf("%.2f",$ymin));
    $extents = $cr->text_extents(0);
    $cr->move_to($left+$offset-5-$extents->{width}, $top+$offset+$space+int(abs($ymax)/$yrange*$height)+$fontheight/2-2);
    $cr->show_text(0);

    $cr->save;

    #labels
    $cr->set_font_size (14);
    $font_extents = $cr->font_extents;
    $fontheight = $font_extents->{height};

    #add type
    $cr->set_source_rgba(0, 0, 0, 0.5);
    $extents = $cr->text_extents(uc($type));
    $cr->arc($offset/2+$extents->{width}/2, $offset-5, 10, 0, 2*$PI);
    $cr->fill;
    $cr->set_source_rgba(1, 1, 1, 1);
    $cr->move_to($offset/2-($type eq 'm' ? 1 : 0), $offset);
    $cr->show_text(uc($type));

    #axis labels
    $cr->set_source_rgba(0, 0, 0, 1);
    $extents = $cr->text_extents($xlab.'  ('.$var1.'%)');
    $cr->move_to($left+$offset+$height/2-$extents->{width}/2+$space, $top+$offset+$height+$fontheight+15+2*$space);
    $cr->show_text($xlab.'  ('.$var1.'%)');
    $cr->rotate($PI * 3 / 2);
    $extents = $cr->text_extents($ylab.'  ('.$var2.'%)');
    $cr->move_to(-($top+$offset+$height/2+$extents->{width}/2)+$space,$offset);
    $cr->show_text($ylab.'  ('.$var2.'%)');

    $cr->restore;

    #draw dots
    $cr->set_antialias('default');
    $cr->set_font_size (10);
    foreach my $i (0..$num) {
        $cr->set_source_rgba(@{$data_info->[$i]->[3]});
        $cr->arc(($left+$offset+$space+int(($data->[0]->[$i]+abs($xmin))/$xrange*$height)), ($space+$top+$offset+int(($data->[1]->[$i]+abs($ymin))/$yrange*$height)), $size, 0, 2*$PI);
        $cr->fill;
    }
    $cr->set_source_rgba(0, 0, 0, 1);
    foreach my $i (0..$num) {
        $extents = $cr->text_extents($data_info->[$i]->[1]);
	$cr->move_to(($left+$offset+$space+int(($data->[0]->[$i]+abs($xmin))/$xrange*$height))+$size+1, ($space+$top+$offset+int(($data->[1]->[$i]+abs($ymin))/$yrange*$height))+$size*2);
	$cr->show_text($data_info->[$i]->[1]);
    }

    #draw legend
    my %labels;
    foreach  my $i (0..$num) {
	$labels{$data_info->[$i]->[1]} = $data_info->[$i]->[2];
    }
    $cr->set_font_size(10);
    $fontheight = $font_extents->{height};
    $cr->set_source_rgba(0, 0, 0, 1);
    my $x = $left+$offset+$space;
    my $y = int($offset/2);
    foreach my $n (sort {$a <=> $b} keys %labels) {
	if($x+$cr->text_extents($n.' - '.$labels{$n})->{width}+15 >= $left+$offset+$space+$height) {
	    $x = $left+$offset+$space;
	    $y += $fontheight;
	}
	$cr->move_to($x,$y);
	$cr->show_text($n.' - '.$labels{$n});
	$x += $cr->text_extents($n.' - '.$labels{$n})->{width}+15;

    }

    #write image
    $cr->show_page;
    return $surface;
}

sub createOddsRatioPlot {
    my ($data,$title,$xlab,$ylab,$file) = @_;

    my @yvalues = (0.5,0.78,1.00,1.23,1.5);

    my @linecol = (0, 0, 0, 0.4);
    my @helplinecol1 = (1,1,1, 0.9);
    my @helplinecol2 = (1,1,1, 0.5);

    #create new image
    my $size   = 40;
    my $offset = 20;
    my $left   = 35;
    my $right = 90;
    my $bottom = 20;
    my $top = 0;
    my $height = 100;
    my $width = $size*10;
    my $space = 20;
    my $surface = Cairo::ImageSurface->create('argb32', $left+$offset*2+$width+$right,$top+$bottom+$offset*2+$height); #format, width, height
    my $cr = Cairo::Context->create($surface);

    my ($font_extents,$extents,$fontheight,$fontdescent);

    #background
    $cr->rectangle(0, 0, $left+$offset*2+$width+$right,$top+$bottom+$offset*2+$height);
    $cr->set_source_rgba(1, 1, 1, 1);
    $cr->fill;

    #fonts
    $cr->select_font_face ('sans', 'normal', 'normal');

    $cr->save;

    #set up work space
    my ($dx, $dy);
    $cr->set_antialias('none');
    $cr->set_line_width(1);

    #background for plot
    $cr->rectangle($left+$offset, $top+$offset, $width, $height);
    $cr->set_source_rgba(0.95, 0.95, 0.95, 1);
    $cr->fill;

    #right side marks
    $cr->set_source_rgba(255/255, 127/255, 127/255, 0.6);
    $cr->rectangle($left+$offset+$width+8, $top+$offset, 3, 0.77/2*$height);
    $cr->fill;
    $cr->rectangle($left+$offset+$width+8, $top+$offset+$height-0.78/2*$height, 3, 0.78/2*$height);
    $cr->fill;

    #get infos
    my $num = scalar(keys %$data)-1;

    #draw ticks
    #x-axis
    $cr->set_source_rgba(0, 0, 0, 0.8);
    foreach my $i (0..$num) {
	$cr->move_to($left+$offset+$size/2+$i*$size, $top+$offset+$height);
	$cr->line_to($left+$offset+$size/2+$i*$size, $top+$offset+$height+3);
    }
    $cr->stroke;
    #y-axis
    foreach my $i (@yvalues) {
	$cr->move_to($left+$offset, $top+$offset+$height-$i/2*$height);
	$cr->line_to($left+$offset-3, $top+$offset+$height-$i/2*$height);
    }
    $cr->stroke;

    #helplines
    #x-axis
    $cr->set_source_rgba(@helplinecol1);
    foreach my $i (0..$num) {
	$cr->move_to($left+$offset+$size/2+$i*$size, $top+$offset);
	$cr->line_to($left+$offset+$size/2+$i*$size, $top+$offset+$height);
    }
    $cr->stroke;
    #yaxis
    foreach my $i (@yvalues) {
	$cr->set_source_rgba(0, 0, 0, ($i == 0.5 || $i == 1.00 || $i == 1.50 ? 0.1 : 0.3));
	$cr->move_to($left+$offset, $top+$offset+$height-$i/2*$height);
	$cr->line_to($left+$offset+$width, $top+$offset+$height-$i/2*$height);
	$cr->stroke;
    }

    $cr->set_antialias('default');

    #tick labels
    $cr->set_source_rgba(0, 0, 0, 0.8);
    $cr->set_font_size(10);
    $font_extents = $cr->font_extents;
    $fontheight = $font_extents->{height};
    #x-axis
    my $xcur = 0;
    foreach my $dn (map {join("/",(m/../g ))} sort keys %$data) {
	$extents = $cr->text_extents($dn);
	$cr->move_to($left+$offset+$size/2-$extents->{width}/2-1+$size*$xcur++, $top+$offset+$height+$fontheight+2);
	$cr->show_text($dn);
    }
    #y-axis
    foreach my $i (@yvalues) {
	$cr->set_source_rgba(0, 0, 0, ($i == 0.5 || $i == 1.00 || $i == 1.50 ? 0.5 : 0.8));
	$extents = $cr->text_extents(sprintf("%.2f",$i));
	$cr->move_to($left+$offset-5-$extents->{width}, $top+$offset+$height-$i/2*$height+$fontheight/2-2);
	$cr->show_text(sprintf("%.2f",$i));
    }

    #label on right side
    $cr->set_source_rgba(0, 0, 0, 1);
    $extents = $cr->text_extents('Over-represented');
    $cr->move_to($left+$offset+$width+15, $top+$offset+$height-1.6/2*$height+$fontheight/2-2);
    $cr->show_text('Over-represented');
    $extents = $cr->text_extents('Under-represented');
    $cr->move_to($left+$offset+$width+15, $top+$offset+$height-0.4/2*$height+$fontheight/2-2);
    $cr->show_text('Under-represented');

    $cr->save;

    #labels
    $cr->set_font_size (14);
    $font_extents = $cr->font_extents;
    $fontheight = $font_extents->{height};

    #axis labels
    #x-axis
    $cr->set_source_rgba(0, 0, 0, 1);
    $extents = $cr->text_extents($xlab);
    $cr->move_to($left+$offset+$width/2-$extents->{width}/2, $top+$offset+$height+$fontheight+15);
    $cr->show_text($xlab);
    #y-axis
    $cr->rotate($PI * 3 / 2);
    $extents = $cr->text_extents($ylab);
    $cr->move_to(-($top+$offset+$height/2+$extents->{width}/2),$offset);
    $cr->show_text($ylab);

    $cr->restore;

    #draw dots
    $cr->set_antialias('default');
    $xcur = 0;
    foreach my $dn (sort keys %$data) {
	if($data->{$dn} > 1.23 || $data->{$dn} < 0.78) {
	    $cr->set_source_rgba(255/255, 127/255, 127/255, 1);
	} else {
	    $cr->set_source_rgba(127/255, 127/255, 255/255, 1);
	}
        $cr->arc($left+$offset+$size/2+$size*$xcur++, $top+$offset+$height-$data->{$dn}/2*$height, 5, 0, 2*$PI);
        $cr->fill;
    }

    #write image
    $cr->show_page;
    return $surface;
}

sub convertToBoxValues {
    my ($data,$niceval) = @_;
    my ($xmax,$ymax,@matrix);
    $xmax = $ymax = 0;
    foreach my $i (sort {$a <=> $b} keys %$data) {
	$xmax++;
	push(@matrix,[$i,$data->{$i}->{min},$data->{$i}->{p25},$data->{$i}->{median},$data->{$i}->{p75},$data->{$i}->{max}]);
	$ymax = &max($ymax,$data->{$i}->{max});
    }

    if($niceval) {
        $ymax = sprintf("%d",($ymax/$niceval)+1)*$niceval if($ymax % $niceval);
    }

    return (\@matrix,$xmax,$ymax);
}

sub createBoxPlot {
    my ($matrix,$xmax,$ymax,$title,$xlab,$ylab,$file,$zero,$add,$bin) = @_;
    $bin = ($bin ? $bin : 1);
    $zero = 0 unless($zero);
    $add = '' unless(defined $add);
    if($xmax != 100) {
	$xmax = 100;
    }
    $ymax = 1 unless($ymax);
#    die Dumper $matrix;


    my @col0 = (178/255, 178/255, 255/255); #b2b2ff
    my @col1 = (255/255, 178/255, 178/255); #ffb2b2
    my @col3 = (127/255, 127/255, 255/255); #7f7fff
    my @col4 = (255/255, 127/255, 127/255); #ff7f7f
    my @linecol = (0, 0, 0, 0.4);
    my @linecol0 = (@col3, 1);
    my @linecol1 = (@col4, 1);
    my @boxcol = (@col3, 1);
    my @whiscol = (@col0, 0.9);
    my @medcol = (0,0,0, 0.5);
    my @helplinecol1 = (1,1,1, 0.9);
    my @helplinecol2 = (1,1,1, 0.5);

    #create new image
    my $size   = 6;
    my $offset = 20;
    my $left   = 25;
    my $bottom = 25;
    my $top = 5;
    my $height = 300;
    my $surface = Cairo::ImageSurface->create('argb32', $left+$offset*2+($xmax+$zero)*$size,$bottom+$offset*2+$height); #format, width, height
    my $cr = Cairo::Context->create($surface);

    my ($font_extents,$extents,$fontheight,$fontdescent);

    #background
    $cr->rectangle(0, 0, $left+$offset*2+($xmax+$zero)*$size,$bottom+$offset*2+2*200+20);
    $cr->set_source_rgba(1, 1, 1, 1);
    $cr->fill;

    #fonts
    $cr->select_font_face ('sans', 'normal', 'normal');
#    $cr->set_font_size (30);

    $cr->save;

    #set up work space
    $cr->set_antialias('none');
    $cr->set_line_width(1);

    #background for plot
    $cr->rectangle($left+$offset, $top+$offset, ($xmax+$zero)*$size-1, $height);
    $cr->set_source_rgba(0.95, 0.95, 0.95, 1);
    $cr->fill;

    #draw legend
    $cr->set_font_size(10);
#    $font_extents = $cr->font_extents;
    my $x = $left+$offset+$size*50;
    foreach my $v ([\@whiscol,'Min/Max value'],[\@boxcol,'25th to 75th percentile'],[\@medcol,'Median']) {
	$cr->set_antialias('none');
	$cr->set_source_rgba(@{$v->[0]});
	$cr->rectangle($x, $top+5, 10, 10);
	$cr->fill;
	$x += 15;
	$cr->set_antialias('default');
	$cr->move_to($x,$top+5+9);
	$cr->set_source_rgba(0, 0, 0, 0.8);
	$cr->show_text($v->[1]);
	$x += $cr->text_extents($v->[1])->{width}+15;
    }

    $cr->set_antialias('none');

    #draw ticks
    #x-axis
    $cr->set_source_rgba(0, 0, 0, 0.8);
#    $cr->move_to($left+$offset+int($size/2+1), $top+$offset+$height);
#    $cr->line_to($left+$offset+int($size/2+1), $top+$offset+$height+3);
#    $cr->move_to($left+$offset+int($size/2+1), $top+$offset+$height+$space);
#    $cr->line_to($left+$offset+int($size/2+1), $top+$offset+$height+$space-3);
    foreach my $i (1..9) {
        $cr->move_to($left+$offset+int($size/2)+$size*10*$i-($zero ? 0 : $size)-1, $top+$offset+$height);
	$cr->line_to($left+$offset+int($size/2)+$size*10*$i-($zero ? 0 : $size)-1, $top+$offset+$height+3);
#	$cr->move_to($left+$offset+int($size/2)+$size*10*$i-($zero ? 0 : $size)-1, $top+$offset);
#	$cr->line_to($left+$offset+int($size/2)+$size*10*$i-($zero ? 0 : $size)-1, $top+$offset-3);
    }
    $cr->stroke;
    #y-axis
    foreach my $j (0..4) {
	$cr->move_to($left+$offset, $top+$offset+$height*$j/4-($j ? 1 : 0));
	$cr->line_to($left+$offset-3, $top+$offset+$height*$j/4-($j ? 1 : 0));
#	$cr->move_to($left+$offset+($xmax+$zero)*$size, $top+$offset+$height*$j/4-($j ? 1 : 0));
#	$cr->line_to($left+$offset+($xmax+$zero)*$size+3, $top+$offset+$height*$j/4-($j ? 1 : 0));
    }
    $cr->stroke;

    #helplines
    $cr->set_source_rgba(@helplinecol1);
    foreach my $j (1..3) {
        $cr->move_to($left+$offset, $top+$offset+$height*$j/4-($j ? 1 : 0));
        $cr->line_to($left+$offset+($xmax+$zero)*$size, $top+$offset+$height*$j/4-($j ? 1 : 0));
    }
    $cr->stroke;

    $cr->set_antialias('default');

    #tick labels
    $cr->set_source_rgba(0, 0, 0, 0.8);
    $cr->set_font_size(10);
    $font_extents = $cr->font_extents;
    $fontheight = $font_extents->{height};
    #x-axis
#    $extents = $cr->text_extents(1);
#    $cr->move_to($left+$offset+int($size/2+1)-$extents->{width}, $top+$offset+$height+$fontheight+2);
#    $cr->show_text(1);
    foreach my $i (1..9) {
        $extents = $cr->text_extents($i*10*$bin);
	$cr->move_to($left+$offset+int($size/2+1)+$size*10*$i-($zero ? 0 : $size)-$extents->{width}/2-1-($i == 1 ? 1 : 0), $top+$offset+$height+$fontheight+2);
        $cr->show_text($i*10*$bin);
    }
    #y-axis
    foreach my $j (0..4) {
	$extents = $cr->text_extents(&addCommas($ymax*$j/4));
	$cr->move_to($left+$offset-5-$extents->{width}, $top+$offset+$fontheight/2-2+$height*(4-$j)/4);
	$cr->show_text(&addCommas($ymax*$j/4));
    }

    $cr->save;

    #axis labels
    $cr->set_source_rgba(0, 0, 0, 1);
    $cr->set_font_size (14);
    $font_extents = $cr->font_extents;
    $fontheight = $font_extents->{height};
    $extents = $cr->text_extents($xlab.($bin>1 ? ' (Bin size: '.$bin.($add ? $add.')' : '') : ''));
    $cr->move_to($left+$offset+($xmax+$zero)*$size/2-$extents->{width}/2, $top+$offset+$height+$fontheight+15);
    $cr->show_text($xlab.($bin>1 ? ' (Bin size: '.$bin.($add ? $add.')' : '') : ''));
    $cr->rotate($PI * 3 / 2);
    $extents = $cr->text_extents($ylab);
    $cr->move_to(-($top+$offset+$height/2+$extents->{width}/2),$offset);
    $cr->show_text($ylab);

    $cr->restore;

    #draw boxes
    my $factor = $height/$ymax;
    $cr->set_antialias('none');
    foreach my $v (@$matrix) {
	#wiskers
	$cr->set_source_rgba(@whiscol);
	if($v->[1] != $v->[2]) {
	    $cr->move_to($left+$offset+$size*$v->[0]+1, $top+$offset+$height-$v->[1]*$factor-1);
	    $cr->line_to($left+$offset+$size*$v->[0]+$size-2, $top+$offset+$height-$v->[1]*$factor-1);
	    $cr->stroke;
	}
	if($v->[4] != $v->[5]) {
	    $cr->move_to($left+$offset+$size*$v->[0]+1, $top+$offset+$height-$v->[5]*$factor);
	    $cr->line_to($left+$offset+$size*$v->[0]+$size-2, $top+$offset+$height-$v->[5]*$factor);
	    $cr->stroke;
	}
	$cr->save;
	$cr->set_dash(1,4,3);
	if($v->[1] != $v->[2]) {
	    $cr->move_to($left+$offset+$size*$v->[0]+int($size/2)-1, $top+$offset+$height-$v->[2]*$factor);
	    $cr->line_to($left+$offset+$size*$v->[0]+int($size/2)-1, $top+$offset+$height-$v->[1]*$factor);
	    $cr->stroke;
	}
	if($v->[4] != $v->[5]) {
	    $cr->move_to($left+$offset+$size*$v->[0]+int($size/2)-1, $top+$offset+$height-$v->[5]*$factor);
	    $cr->line_to($left+$offset+$size*$v->[0]+int($size/2)-1, $top+$offset+$height-$v->[4]*$factor-1);
	    $cr->stroke;
	}
	$cr->restore;
	#box
	if(($v->[2] != $v->[3]) || ($v->[4] != $v->[3])) {
	    $cr->set_source_rgba(@whiscol);
	    $cr->rectangle($left+$offset+$size*$v->[0], $top+$offset+$height-$v->[2]*$factor, $size-1, -($v->[4]-$v->[2])*$factor);
	    $cr->fill;
	    $cr->stroke;
	    $cr->set_source_rgba(@boxcol);
	    $cr->rectangle($left+$offset+$size*$v->[0], $top+$offset+$height-$v->[2]*$factor, $size-2, -($v->[4]-$v->[2])*$factor);
	    $cr->stroke;
	} else {
	    $cr->set_source_rgba(@boxcol);
	    $cr->move_to($left+$offset+$size*$v->[0], $top+$offset+$height-$v->[3]*$factor);
	    $cr->line_to($left+$offset+$size*$v->[0]+$size-1, $top+$offset+$height-$v->[3]*$factor);
	    $cr->stroke;
	}
	#median
	$cr->set_source_rgba(@medcol);
	$cr->move_to($left+$offset+$size*$v->[0]+1, $top+$offset+$height-$v->[3]*$factor);
	$cr->line_to($left+$offset+$size*$v->[0]+$size-2, $top+$offset+$height-$v->[3]*$factor);
	$cr->stroke;
    }

    #write image
    $cr->show_page;
    return $surface;
}

sub convertToBarValues {
    my ($data,$niceval,$start,$max) = @_;
    my ($xmax,$ymax,@matrix,$tmp);
    $xmax = $ymax = 0;

    #get xmax value
    if($max) {
	$xmax = $max;
    } else {
	foreach my $q (keys %$data) {
	    $xmax = &max($xmax,$q);
	}
    }
    if($niceval) {
        $xmax = sprintf("%d",($xmax/$niceval)+1)*$niceval if($xmax % $niceval);
    }

    #get matrix values
    foreach my $q ($start..$xmax) {
	$tmp = (exists $data->{$q} ? $data->{$q} : 0);
	$ymax = &max($ymax,$tmp);
	push(@matrix,$tmp);
    }

    $ymax = sprintf("%d",($ymax/4)+1)*4 if($ymax % 4);

    return (\@matrix,$xmax,$ymax);
}

sub createBarPlot {
    my ($matrix,$xmax,$ymax,$title,$xlab,$ylab,$file,$zero) = @_;

    my @col0 = (178/255, 178/255, 255/255); #b2b2ff
    my @col1 = (255/255, 178/255, 178/255); #ffb2b2
    my @col3 = (127/255, 127/255, 255/255); #7f7fff
    my @col4 = (255/255, 127/255, 127/255); #ff7f7f
    my @linecol = (0, 0, 0, 0.4);
    my @linecol0 = (@col3, 1);
    my @linecol1 = (@col4, 1);
    my @barcol0 = (@col3, 1);
    my @barcol1 = (@col4, 1);
    my @helplinecol1 = (1,1,1, 0.9);
    my @helplinecol2 = (1,1,1, 0.5);

    #create new image
    my $size   = ($xmax <= 50 ? 10 : ($xmax <= 100 ? 6 : 3));
    my $offset = 20;
    my $left   = 25;
    my $bottom = 15;
    my $top = 0;
    my $height = 200;
    my $surface = Cairo::ImageSurface->create('argb32', $left+$offset*2+($xmax+$zero)*$size,$bottom+$offset*2+$height); #format, width, height
    my $cr = Cairo::Context->create($surface);

    my ($font_extents,$extents,$fontheight,$fontdescent);

    #background
    $cr->rectangle(0, 0, $left+$offset*2+($xmax+$zero)*$size,$bottom+$offset*2+2*200+20);
    $cr->set_source_rgba(1, 1, 1, 1);
    $cr->fill;

    #fonts
    $cr->select_font_face ('sans', 'normal', 'normal');
#    $cr->set_font_size (30);

    $cr->save;

    #set up work space
    my ($dx, $dy);
    $cr->set_antialias('none');
    $cr->set_line_width(1);

    #background for plot
    $cr->rectangle($left+$offset, $top+$offset, ($xmax+$zero)*$size-1, $height);
    $cr->set_source_rgba(0.95, 0.95, 0.95, 1);
    $cr->fill;

    #draw ticks
    #x-axis
    $cr->set_source_rgba(0, 0, 0, 0.8);
    foreach my $i (($zero ? 0 : 1)..$xmax) {
	if(($i%5) == 0 && $i > 1 && $i < $xmax) {
	    $cr->move_to($left+$offset+int($size/2)+$size*$i-($zero ? 0 : $size)-1, $top+$offset+$height);
	    $cr->line_to($left+$offset+int($size/2)+$size*$i-($zero ? 0 : $size)-1, $top+$offset+$height+3);
	} else {
	    $cr->move_to($left+$offset+int($size/2)+$size*$i-($zero ? 0 : $size)-1, $top+$offset+$height);
	    $cr->line_to($left+$offset+int($size/2)+$size*$i-($zero ? 0 : $size)-1, $top+$offset+$height+1);
	}
    }
    $cr->stroke;

    #y-axis
    $cr->move_to($left+$offset, $top+$offset);
    $cr->line_to($left+$offset-3, $top+$offset);
    $cr->move_to($left+$offset, $top+$offset+$height-1);
    $cr->line_to($left+$offset-3, $top+$offset+$height-1);
    $cr->stroke;

    #helplines
    $cr->set_source_rgba(@helplinecol1);
    foreach my $j (1..3) {
        $cr->move_to($left+$offset, $top+$offset+$height*$j/4-($j ? 1 : 0));
        $cr->line_to($left+$offset+($xmax+$zero)*$size, $top+$offset+$height*$j/4-($j ? 1 : 0));
    }
    $cr->stroke;

    $cr->set_antialias('default');

    #tick labels
    $cr->set_source_rgba(0, 0, 0, 0.8);
    $cr->set_font_size(10);
    $font_extents = $cr->font_extents;
    $fontheight = $font_extents->{height};
    #x-axis
    foreach my $i (($zero ? 0 : 1)..$xmax) {
	if(($i%5) == 0 && $i > 1 && $i < $xmax) {
	    $extents = $cr->text_extents($i);
	    $cr->move_to($left+$offset+int($size/2+1)+$size*$i-($zero ? 0 : $size)-$extents->{width}/2-1-($i == 1 ? 1 : 0), $top+$offset+$height+$fontheight+2);
	    $cr->show_text($i);
	}
    }
    #y-axis
    $extents = $cr->text_extents(&addCommas($ymax));
    $cr->move_to($left+$offset-5-$extents->{width}, $top+$offset+$fontheight/2-2);
    $cr->show_text(&addCommas($ymax));
    $extents = $cr->text_extents(0);
    $cr->move_to($left+$offset-5-$extents->{width}, $top+$offset+$fontheight/2-2+$height);
    $cr->show_text(0);

    $cr->save;

    #labels
    $cr->set_font_size (14);
    $font_extents = $cr->font_extents;
    $fontheight = $font_extents->{height};

    #axis labels
    $cr->set_source_rgba(0, 0, 0, 1);
    $extents = $cr->text_extents($xlab);
    $cr->move_to($left+$offset+($xmax+$zero)*$size/2-$extents->{width}/2, $top+$offset+$height+$fontheight+15);
    $cr->show_text($xlab);
    $cr->rotate($PI * 3 / 2);
    $extents = $cr->text_extents($ylab);
    $cr->move_to(-($top+$offset+$height/2+$extents->{width}/2),$offset);
    $cr->show_text($ylab);

    $cr->restore;

    #draw boxes
    $cr->set_antialias('none');
    foreach my $pos (0..$xmax-($zero ? 0 : 1)) {
        next unless($matrix->[$pos+($zero ? 0 : 1)]);
        my $tmp = $matrix->[$pos+($zero ? 0 : 1)] / $ymax;
	#unique
        if($tmp) {
            $cr->set_source_rgba(@barcol0);
            $cr->rectangle($left+$offset+($pos+($zero ? 0 : 1))*$size, $top+$offset+$height, $size-1, -$tmp*$height);
            $cr->fill;
        }
    }

    #write image
    $cr->show_page;
    return $surface;
}

sub convertOdToStackBinMatrix {
    my ($data,$stacks,$min,$max,$nonice) = @_;

    my ($num,$ymax,$xmax,$xmin,$step,%vals,%sums,$sum,@matrix,$bin,$tmpbin);

    #make nice xmax value
    if(defined $max) {
	$xmax = $max;
    } else {
	$xmax = (sort {$b <=> $a} keys %$data)[0];
    }
    $bin = &getBinVal($xmax);
    $xmax = $bin*100;
    $xmin = (defined $min ? $min : 0);

    #get data to bin and find y axis max value
    $ymax = 0;
    foreach my $s (0..$stacks-1) {
	$sums{$s} = 0;
    }
    $sum = 0;
    $tmpbin = $bin;
    foreach my $i ($xmin..$xmax) {
	foreach my $s (0..$stacks-1) {
	    next unless(exists $data->{$i}->{$s});
	    $sums{$s} += $data->{$i}->{$s};
	    $sum += $data->{$i}->{$s};
	}
	if(--$tmpbin <= 0) {
	    $tmpbin = $bin;
	    $ymax = &max($ymax,$sum);
	    $sum = 0;
	    foreach my $s (0..$stacks-1) {
		push(@{$matrix[$s]},$sums{$s});
		$sums{$s} = 0;
	    }
	}
    }

    #make nice ymax value
    unless($nonice) {
	$ymax = sprintf("%d",($ymax/4)+1)*4 if($ymax % 4);
#        $step = ($ymax <= 10 ? 10 : ($ymax < 40 ? 40 : ($ymax < 100 ? 100 : ($ymax < 1000 ? 100 : 100))));
#        $ymax = sprintf("%d",($ymax/$step)+1)*$step if($ymax % $step);
    }

    return (\@matrix,$xmax,$ymax,$stacks);
}

sub createStackBarPlot {
    my ($matrix,$xmax,$ymax,$stacks,$title,$xlab,$ylab,$file,$zero,$add) = @_;

    my $bin = 1;
    if($xmax > 100) {
	$bin = $xmax / 100;
	$xmax = 100;
    }

    my @legend = ('Exact dupl.','5\' dupl.','3\' dupl.','Rev. compl. exact dupl.','Rev. compl. 5\'/3\' dupl.');
    my @cols = ([69/255, 114/255, 167/255, 1],
		[137/255, 1165/255, 78/255, 1],
		[170/255, 70/255, 67/255, 1],
		[147/255, 169/255, 207/255, 1],
		[51/255, 102/255, 102/255, 1]);
    my @barcol = (127/255, 127/255, 255/255, 1); #b2b2ff
    my @meancol = (255/255, 127/255, 127/255, 1); #ffb2b2
    my @stdcol = (178/255, 178/255, 255/255, 0.8); #7f7fff
    my @std1col = (0, 0, 0, 0.02); #ff7f7f
    my @std2col = (0, 0, 0, 0.02); #ff7f7f
    my @linecol = (0, 0, 0, 0.4);
    my @helplinecol = (1, 1, 1, 0.9);
    my @background = (0.95, 0.95, 0.95, 1);
    my @tickcol = (0, 0, 0, 0.8);
    my @labelcol = (0, 0, 0, 1);

    #create new image
    my $size   = 6;
    my $offset = 20;
    my $left   = 40;
    my $bottom = 15;
    my $top = 20;
    my $height = 200;
    my $surface = Cairo::ImageSurface->create('argb32', $left+$offset*2+($xmax+$zero)*$size,$bottom+$top+$offset*2+$height); #format, width, height
    my $cr = Cairo::Context->create($surface);

    my ($font_extents,$extents,$fontheight,$fontdescent);

    #background
    $cr->rectangle(0, 0, $left+$offset*2+($xmax+$zero)*$size,$bottom+$offset*2+2*200+20);
    $cr->set_source_rgba(1, 1, 1, 1);
    $cr->fill;

    #fonts
    $cr->select_font_face ('sans', 'normal', 'normal');

    $cr->save;

    #set up work space
    $cr->set_antialias('none');
    $cr->set_line_width(1);

    #background for plot
    $cr->rectangle($left+$offset, $top+$offset, ($xmax+$zero)*$size-1, $height);
    $cr->set_source_rgba(@background);
    $cr->fill;

    #draw legend
    $cr->set_font_size(10);
#    $font_extents = $cr->font_extents;
    my $x = $left+$offset+$size*100-5;
    foreach my $i (reverse (0..scalar(@legend)-1)) {
	$cr->set_antialias('default');
	$x -= $cr->text_extents($legend[$i])->{width};
	$cr->move_to($x,$top+5+9);
	$cr->set_source_rgba(@tickcol);
	$cr->show_text($legend[$i]);
	$x -= 15;
	$cr->set_antialias('none');
	$cr->set_source_rgba(@{$cols[$i]});
	$cr->rectangle($x, $top+5, 10, 10);
	$cr->fill;
	$x -= 15;
    }

    #draw ticks
    $cr->set_antialias('none');
    #x-axis
    $cr->set_source_rgba(@tickcol);
    foreach my $i (($zero ? 0 : 1)..$xmax) {
	if(($i%5) == 0 && $i > 1 && $i < $xmax) {
	    $cr->move_to($left+$offset+int($size/2)+$size*$i-($zero ? 0 : $size)-1, $top+$offset+$height);
	    $cr->line_to($left+$offset+int($size/2)+$size*$i-($zero ? 0 : $size)-1, $top+$offset+$height+3);
	} else {
	    $cr->move_to($left+$offset+int($size/2)+$size*$i-($zero ? 0 : $size)-1, $top+$offset+$height);
	    $cr->line_to($left+$offset+int($size/2)+$size*$i-($zero ? 0 : $size)-1, $top+$offset+$height+1);
	}
    }
    $cr->stroke;

    #y-axis
    $cr->move_to($left+$offset, $top+$offset);
    $cr->line_to($left+$offset-3, $top+$offset);
    $cr->move_to($left+$offset, $top+$offset+$height-1);
    $cr->line_to($left+$offset-3, $top+$offset+$height-1);
    $cr->stroke;

    #helplines
    $cr->set_source_rgba(@helplinecol);
    foreach my $j (1..3) {
        $cr->move_to($left+$offset, $top+$offset+$height*$j/4-($j ? 1 : 0));
        $cr->line_to($left+$offset+($xmax+$zero)*$size, $top+$offset+$height*$j/4-($j ? 1 : 0));
    }
    $cr->stroke;

    $cr->set_antialias('default');

    #tick labels
    $cr->set_source_rgba(@tickcol);
    $cr->set_font_size(10);
    $font_extents = $cr->font_extents;
    $fontheight = $font_extents->{height};
    #x-axis
    foreach my $i (($zero ? 0 : 1)..$xmax) {
	if(($i%10) == 0 && $i > 1 && $i < $xmax) {
	    $extents = $cr->text_extents($i*$bin);
	    $cr->move_to($left+$offset+int($size/2+1)+$size*$i-($zero ? 0 : $size)-$extents->{width}/2-1-($i == 1 ? 1 : 0), $top+$offset+$height+$fontheight+2);
	    $cr->show_text($i*$bin);
	}
    }
    #y-axis
    $extents = $cr->text_extents(&addCommas($ymax));
    $cr->move_to($left+$offset-5-$extents->{width}, $top+$offset+$fontheight/2-2);
    $cr->show_text(&addCommas($ymax));
    $extents = $cr->text_extents(0);
    $cr->move_to($left+$offset-5-$extents->{width}, $top+$offset+$fontheight/2-2+$height);
    $cr->show_text(0);

    $cr->save;

    #labels
    $cr->set_font_size(14);
    $font_extents = $cr->font_extents;
    $fontheight = $font_extents->{height};

    #axis labels
    $cr->set_source_rgba(@labelcol);
    $extents = $cr->text_extents($xlab.($bin>1 ? ' (Bin size: '.$bin.($add ? $add.')' : '') : ''));
    $cr->move_to($left+$offset+($xmax+$zero)*$size/2-$extents->{width}/2, $top+$offset+$height+$fontheight+15);
    $cr->show_text($xlab.($bin>1 ? ' (Bin size: '.$bin.($add ? $add.')' : '') : ''));
    $cr->rotate($PI * 3 / 2);
    $extents = $cr->text_extents($ylab.($bin>1 ? ' (per bin)' : ''));
    $cr->move_to(-($top+$offset+$height/2+$extents->{width}/2+($bin>1 ? 12 : 0)),$offset+10);
    $cr->show_text($ylab.($bin>1 ? ' (per bin)' : ''));

    $cr->restore;

    #draw boxes
    $cr->set_antialias('none');
    foreach my $pos (0..$xmax-($zero ? 0 : 1)) {
	my $tmp = 0;
	foreach my $s (0..$stacks-1) {
	    next unless($matrix->[$s]->[$pos]);
	    my $cur = $matrix->[$s]->[$pos] / $ymax;
	    $cr->set_source_rgba(@{$cols[$s]});
	    if($cur) {
		$cr->rectangle($left+$offset+$pos*$size, $top+$offset+$height-$tmp*$height, $size-1, -$cur*$height);
		$cr->fill;
	    }
	    $tmp += $cur;
	}
    }

    #write image
    $cr->show_page;
    return $surface;
}

sub header {
    return '<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN" "http://www.w3.org/TR/html4/loose.dtd">
<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=UTF-8">
<title>PRINSEQ-'.$WHAT.' Report</title>
<style type="text/css">
<!--/* <![CDATA[ */
'.$CSS_STYLE.'
/* ]]> */-->
</style>
</head>
<body>
<center>';
}

sub footer {
    return '</center></body></html>';
}

sub generateHtml {
    my ($in,$out) = @_;
    my ($file,$data,$surface,$html,$png);
    $data = &readGdFile($in);
    my $time = sprintf("%02d/%02d/%04d %02d:%02d:%02d",sub {($_[4]+1,$_[3],$_[5]+1900,$_[2],$_[1],$_[0])}->(localtime));

    $html .= &header();
    $html .= '<h2>PRINSEQ-'.$WHAT.' v'.$VERSION.' HTML Report&nbsp;&nbsp;&nbsp;</h2>[Generated: '.$time.']<br /><br />';
    $html .= '<div class="info-panel">';

    #input info
    if(exists $data->{numseqs}) {
        $html .= '<div class="info-header"><span class="info-header-title">Input Information</span></div>';
        $html .= '<div class="info-content"><table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td class="info-table-type">Input file(s):</td><td class="info-table-value">'.($data->{filename1} ? &convertIntToString($data->{filename1}) : '-').($data->{filename2} ? ' and '.&convertIntToString($data->{filename2}) : '').'</td></tr><tr><td class="info-table-type">Input format(s):</td><td class="info-table-value">'.($data->{format1} ? uc($data->{format1}) : '-').($data->{format2} ? ' and '.uc($data->{format2}) : '').'</td></tr>';
        if(exists $data->{pairedend} && $data->{pairedend}) {
            my $singletons1 = ($data->{numseqs}||0)-($data->{pairs}||0);
            my $singletons2 = ($data->{numseqs2}||0)-($data->{pairs}||0);
            $html .= '<tr><td class="info-table-type"># Sequences (file 1):</td><td class="info-table-value">'.&addCommas($data->{numseqs}||'-').'</td></tr><tr><td class="info-table-type">Total bases (file 1):</td><td class="info-table-value">'.&addCommas($data->{numbases}||'-').'</td></tr><tr><td class="info-table-type"># Sequences (file 2):</td><td class="info-table-value">'.&addCommas($data->{numseqs2}||'-').'</td></tr><tr><td class="info-table-type">Total bases (file 2):</td><td class="info-table-value">'.&addCommas($data->{numbases2}||'-').'</td></tr><tr><td class="info-table-type"># Pairs:</td><td class="info-table-value">'.&addCommas($data->{pairs}||'-').($data->{pairs} ? '&nbsp;&nbsp;('.sprintf("%.2f",(100*(2*$data->{pairs})/(($data->{numseqs}||0)+($data->{numseqs2}||0)))).'% of sequences)' : '').'</td></tr></tr><tr><td class="info-table-type"># Singletons (file 1):</td><td class="info-table-value">'.&addCommas($singletons1).($singletons1 ? '&nbsp;&nbsp;('.sprintf("%.2f",(100*$singletons1/$data->{numseqs})).'%)' : '').'</td></tr><tr><td class="info-table-type"># Singletons (file 2):</td><td class="info-table-value">'.&addCommas($singletons2).($singletons2 ? '&nbsp;&nbsp;('.sprintf("%.2f",(100*$singletons2/$data->{numseqs2})).'%)' : '').'</td></tr>';
        } else {
            $html .= '<tr><td class="info-table-type"># Sequences:</td><td class="info-table-value">'.&addCommas($data->{numseqs}||'-').'</td></tr><tr><td class="info-table-type">Total bases:</td><td class="info-table-value">'.&addCommas($data->{numbases}||'-').'</td></tr>';
        }
        $html .= '</tbody></table></div><hr>';
    }

    #length plot
    if(exists $data->{counts}->{length} && keys %{$data->{counts}->{length}}) {
        $html .= '<div class="info-header"><span class="info-header-title">Length Distribution</span></div>';
        if(exists $data->{pairedend} && $data->{pairedend}) {
            $html .= '<div class="info-content"><b>File 1</b><br /><table border="0" cellpadding="0" cellspacing="0"> <tbody><tr><td class="info-table-type">Mean sequence length:</td> <td class="info-table-value">'.(exists $data->{stats}->{length}->{mean} ? sprintf("%.2f",$data->{stats}->{length}->{mean}) : '-').' &plusmn; '.(exists $data->{stats}->{length}->{std} ? sprintf("%.2f",$data->{stats}->{length}->{std}) : '-').' bp</td></tr><tr><td class="info-table-type">Minimum length:</td> <td class="info-table-value">'.(exists $data->{stats}->{length}->{min} ? &addCommas($data->{stats}->{length}->{min}) : '-').' bp</td></tr> <tr><td class="info-table-type">Maximum length:</td><td class="info-table-value">'.(exists $data->{stats}->{length}->{max} ? &addCommas($data->{stats}->{length}->{max}) : '-').' bp</td></tr> <tr><td class="info-table-type">Length range:</td><td class="info-table-value">'.(exists $data->{stats}->{length}->{range} ? &addCommas($data->{stats}->{length}->{range}) : '-').' bp</td></tr> <tr><td class="info-table-type">Mode length:</td> <td class="info-table-value">'.(exists $data->{stats}->{length}->{mode} ? &addCommas($data->{stats}->{length}->{mode}) : '-').' bp with '.(exists $data->{stats}->{length}->{modeval} ? &addCommas($data->{stats}->{length}->{modeval}) : '-').' sequences</td></tr></tbody></table><br>';
            $surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts}->{length},1),$data->{stats}->{length},'Length Distribution','Read Length in bp','# Sequences','',0,' bp');
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= &insert_image($png);
            $html .= '</div>';
            $html .= '<div class="info-content"><br /><b>File 2</b><br /><table border="0" cellpadding="0" cellspacing="0"> <tbody><tr><td class="info-table-type">Mean sequence length:</td> <td class="info-table-value">'.(exists $data->{stats2}->{length}->{mean} ? sprintf("%.2f",$data->{stats2}->{length}->{mean}) : '-').' &plusmn; '.(exists $data->{stats2}->{length}->{std} ? sprintf("%.2f",$data->{stats2}->{length}->{std}) : '-').' bp</td></tr><tr><td class="info-table-type">Minimum length:</td> <td class="info-table-value">'.(exists $data->{stats2}->{length}->{min} ? &addCommas($data->{stats2}->{length}->{min}) : '-').' bp</td></tr> <tr><td class="info-table-type">Maximum length:</td><td class="info-table-value">'.(exists $data->{stats2}->{length}->{max} ? &addCommas($data->{stats2}->{length}->{max}) : '-').' bp</td></tr> <tr><td class="info-table-type">Length range:</td><td class="info-table-value">'.(exists $data->{stats2}->{length}->{range} ? &addCommas($data->{stats2}->{length}->{range}) : '-').' bp</td></tr> <tr><td class="info-table-type">Mode length:</td> <td class="info-table-value">'.(exists $data->{stats2}->{length}->{mode} ? &addCommas($data->{stats2}->{length}->{mode}) : '-').' bp with '.(exists $data->{stats2}->{length}->{modeval} ? &addCommas($data->{stats2}->{length}->{modeval}) : '-').' sequences</td></tr></tbody></table><br>';
            $surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts2}->{length},1),$data->{stats2}->{length},'Length Distribution','Read Length in bp','# Sequences','',0,' bp');
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= &insert_image($png);
            $html .= '</div>';
        } else {
            $html .= '<div class="info-content"><table border="0" cellpadding="0" cellspacing="0"> <tbody><tr><td class="info-table-type">Mean sequence length:</td> <td class="info-table-value">'.(exists $data->{stats}->{length}->{mean} ? sprintf("%.2f",$data->{stats}->{length}->{mean}) : '-').' &plusmn; '.(exists $data->{stats}->{length}->{std} ? sprintf("%.2f",$data->{stats}->{length}->{std}) : '-').' bp</td></tr><tr><td class="info-table-type">Minimum length:</td> <td class="info-table-value">'.(exists $data->{stats}->{length}->{min} ? &addCommas($data->{stats}->{length}->{min}) : '-').' bp</td></tr> <tr><td class="info-table-type">Maximum length:</td><td class="info-table-value">'.(exists $data->{stats}->{length}->{max} ? &addCommas($data->{stats}->{length}->{max}) : '-').' bp</td></tr> <tr><td class="info-table-type">Length range:</td><td class="info-table-value">'.(exists $data->{stats}->{length}->{range} ? &addCommas($data->{stats}->{length}->{range}) : '-').' bp</td></tr> <tr><td class="info-table-type">Mode length:</td> <td class="info-table-value">'.(exists $data->{stats}->{length}->{mode} ? &addCommas($data->{stats}->{length}->{mode}) : '-').' bp with '.(exists $data->{stats}->{length}->{modeval} ? &addCommas($data->{stats}->{length}->{modeval}) : '-').' sequences</td></tr></tbody></table><br>';
            $surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts}->{length},1),$data->{stats}->{length},'Length Distribution','Read Length in bp','# Sequences','',0,' bp');
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= &insert_image($png);
            $html .= '</div>';
        }
        $html .= '<hr>';
    }

    #GC content
    if(exists $data->{counts}->{gc} && keys %{$data->{counts}->{gc}}) {
	$html .= '<div class="info-header"><span class="info-header-title">GC Content Distribution</span></div>';
        if(exists $data->{pairedend} && $data->{pairedend}) {
            $html .= '<div class="info-content"><b>File 1</b><br /><table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td class="info-table-type">Mean GC content:</td> <td class="info-table-value">'.(exists $data->{stats}->{gc}->{mean} ? sprintf("%.2f",$data->{stats}->{gc}->{mean}) : '-').' &plusmn; '.(exists $data->{stats}->{gc}->{std} ? sprintf("%.2f",$data->{stats}->{gc}->{std}) : '-').' %</td></tr> <tr><td class="info-table-type">Minimum GC content:</td> <td class="info-table-value">'.(exists $data->{stats}->{gc}->{min} ? $data->{stats}->{gc}->{min} : '-').' %</td></tr> <tr><td class="info-table-type">Maximum GC content:</td> <td class="info-table-value">'.(exists $data->{stats}->{gc}->{max} ? $data->{stats}->{gc}->{max} : '-').' %</td></tr> <tr><td class="info-table-type">GC content range:</td> <td class="info-table-value">'.(exists $data->{stats}->{gc}->{range} ? $data->{stats}->{gc}->{range} : '-').' %</td></tr> <tr><td class="info-table-type">Mode GC content:</td> <td class="info-table-value">'.(exists $data->{stats}->{gc}->{mode} ? $data->{stats}->{gc}->{mode} : '-').' % with '.(exists $data->{stats}->{gc}->{modeval} ? &addCommas($data->{stats}->{gc}->{modeval}) : '-').' sequences</td></tr> </tbody></table><br>';
            $surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts}->{gc},0),$data->{stats}->{gc},'GC Content Distribution','GC Content (0-100%)','Number of Sequences','',1);
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= &insert_image($png);
            $html .= '</div>';
            $html .= '<div class="info-content"><br /><b>File 2</b><br /><table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td class="info-table-type">Mean GC content:</td> <td class="info-table-value">'.(exists $data->{stats2}->{gc}->{mean} ? sprintf("%.2f",$data->{stats2}->{gc}->{mean}) : '-').' &plusmn; '.(exists $data->{stats2}->{gc}->{std} ? sprintf("%.2f",$data->{stats2}->{gc}->{std}) : '-').' %</td></tr> <tr><td class="info-table-type">Minimum GC content:</td> <td class="info-table-value">'.(exists $data->{stats2}->{gc}->{min} ? $data->{stats2}->{gc}->{min} : '-').' %</td></tr> <tr><td class="info-table-type">Maximum GC content:</td> <td class="info-table-value">'.(exists $data->{stats2}->{gc}->{max} ? $data->{stats2}->{gc}->{max} : '-').' %</td></tr> <tr><td class="info-table-type">GC content range:</td> <td class="info-table-value">'.(exists $data->{stats2}->{gc}->{range} ? $data->{stats2}->{gc}->{range} : '-').' %</td></tr> <tr><td class="info-table-type">Mode GC content:</td> <td class="info-table-value">'.(exists $data->{stats2}->{gc}->{mode} ? $data->{stats2}->{gc}->{mode} : '-').' % with '.(exists $data->{stats2}->{gc}->{modeval} ? &addCommas($data->{stats2}->{gc}->{modeval}) : '-').' sequences</td></tr> </tbody></table><br>';
            $surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts2}->{gc},0),$data->{stats2}->{gc},'GC Content Distribution','GC Content (0-100%)','Number of Sequences','',1);
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= &insert_image($png);
            $html .= '</div>';
        } else {
            $html .= '<div class="info-content"><table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td class="info-table-type">Mean GC content:</td> <td class="info-table-value">'.(exists $data->{stats}->{gc}->{mean} ? sprintf("%.2f",$data->{stats}->{gc}->{mean}) : '-').' &plusmn; '.(exists $data->{stats}->{gc}->{std} ? sprintf("%.2f",$data->{stats}->{gc}->{std}) : '-').' %</td></tr> <tr><td class="info-table-type">Minimum GC content:</td> <td class="info-table-value">'.(exists $data->{stats}->{gc}->{min} ? $data->{stats}->{gc}->{min} : '-').' %</td></tr> <tr><td class="info-table-type">Maximum GC content:</td> <td class="info-table-value">'.(exists $data->{stats}->{gc}->{max} ? $data->{stats}->{gc}->{max} : '-').' %</td></tr> <tr><td class="info-table-type">GC content range:</td> <td class="info-table-value">'.(exists $data->{stats}->{gc}->{range} ? $data->{stats}->{gc}->{range} : '-').' %</td></tr> <tr><td class="info-table-type">Mode GC content:</td> <td class="info-table-value">'.(exists $data->{stats}->{gc}->{mode} ? $data->{stats}->{gc}->{mode} : '-').' % with '.(exists $data->{stats}->{gc}->{modeval} ? &addCommas($data->{stats}->{gc}->{modeval}) : '-').' sequences</td></tr> </tbody></table><br>';
            $surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts}->{gc},0),$data->{stats}->{gc},'GC Content Distribution','GC Content (0-100%)','Number of Sequences','',1);
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= &insert_image($png);
            $html .= '</div>';
        }
        $html .= '<hr>';
    }

    #Base quality
    if(exists $data->{quals} || exists $data->{qualsmean} || exists $data->{qualsbin}) {
	$html .= '<div class="info-header"><span class="info-header-title">Base Quality Distribution</span></div><div class="info-content">';
        if(exists $data->{pairedend} && $data->{pairedend}) {
            $html .= '<b>File 1</b><br />';
        }
    }
    if(exists $data->{quals} && keys %{$data->{quals}}) {
	$surface = &createBoxPlot(&convertToBoxValues($data->{quals},4),'Base Quality Distribution','Read position in %','Quality score','');
	$png = '';
	$surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
	$html .= &insert_image($png);
    }
    if(exists $data->{qualsbin} && keys %{$data->{qualsbin}}) {
	$surface = &createBoxPlot(&convertToBoxValues($data->{qualsbin},4),'Base Quality Distribution','Read position in bp','Quality score','',0,'bp',$data->{binval});
	$png = '';
	$surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
        $html .= '<br /><br />' if(exists $data->{quals});
	$html .= &insert_image($png);
    }
    if(exists $data->{qualsmean} && keys %{$data->{qualsmean}}) {
	$surface = &createBarPlot(&convertToBarValues($data->{qualsmean},5,1),'Sequence Quality Distribution','Mean of quality scores per sequence','Number of sequences','',0);
	$png = '';
	$surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
	$html .= '<br /><br />' if(exists $data->{qualsbin});
	$html .= &insert_image($png);
    }
    if(exists $data->{pairedend} && $data->{pairedend}) {
        if(exists $data->{quals} || exists $data->{qualsmean} || exists $data->{qualsbin}) {
            $html .= '<br /><br /><br /><b>File 2</b><br />';
        }
        if(exists $data->{quals2} && keys %{$data->{quals2}}) {
            $surface = &createBoxPlot(&convertToBoxValues($data->{quals2},4),'Base Quality Distribution','Read position in %','Quality score','');
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= &insert_image($png);
        }
        if(exists $data->{qualsbin2} && keys %{$data->{qualsbin2}}) {
            $surface = &createBoxPlot(&convertToBoxValues($data->{qualsbin2},4),'Base Quality Distribution','Read position in bp','Quality score','',0,'bp',$data->{binval});
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= '<br /><br />' if(exists $data->{quals2});
            $html .= &insert_image($png);
        }
        if(exists $data->{qualsmean2} && keys %{$data->{qualsmean2}}) {
            $surface = &createBarPlot(&convertToBarValues($data->{qualsmean2},5,1),'Sequence Quality Distribution','Mean of quality scores per sequence','Number of sequences','',0);
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= '<br /><br />' if(exists $data->{qualsbin2});
            $html .= &insert_image($png);
        }
    }
    if(exists $data->{quals} || exists $data->{qualsmean} || exists $data->{qualsbin}) {
	$html .= '</div><hr>';
    }

    #Ns
    if((exists $data->{counts}->{ns} && keys %{$data->{counts}->{ns}}) || (exists $data->{counts2} && exists $data->{counts2}->{ns} && keys %{$data->{counts2}->{ns}})) {
        $html .= '<div class="info-header"><span class="info-header-title">Occurence of N</span></div><div class="info-content">';
        if(exists $data->{pairedend} && $data->{pairedend}) {
            $html .= '<b>File 1</b><br />';
        }
    }
    if(exists $data->{counts}->{ns} && keys %{$data->{counts}->{ns}}) {
	my $nscount = 0;
	foreach my $n (values %{$data->{counts}->{ns}}) {
	    $nscount += $n;
	}	
	$html .= '<table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td class="info-table-type">Sequences with N:</td> <td class="info-table-value">'.($nscount ? &addCommas($nscount).' &nbsp;('.sprintf("%.2f",100/$data->{numseqs}*$nscount).' %)' : 0).'</td></tr><tr><td class="info-table-type">Max percentage of Ns per sequence:</td> <td class="info-table-value">'.(exists $data->{stats}->{ns}->{max} ? $data->{stats}->{ns}->{max} : 0).' %</td></tr></tbody></table>';
	if($nscount) {
            $surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts}->{ns},1),undef,'Percentage of N\'s (> 0%)','Percentage of N\'s per Read (1-100%)','# Sequences','',0);
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= '<br />'.&insert_image($png);
        }
    }
    if(exists $data->{pairedend} && $data->{pairedend} && exists $data->{counts2}->{ns} && keys %{$data->{counts2}->{ns}}) {
        $html .= '<br /><br /><br /><b>File 2</b><br />';
        my $nscount = 0;
        foreach my $n (values %{$data->{counts2}->{ns}}) {
            $nscount += $n;
        }	
        $html .= '<table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td class="info-table-type">Sequences with N:</td> <td class="info-table-value">'.($nscount ? &addCommas($nscount).' &nbsp;('.sprintf("%.2f",100/$data->{numseqs2}*$nscount).' %)' : 0).'</td></tr><tr><td class="info-table-type">Max percentage of Ns per sequence:</td> <td class="info-table-value">'.(exists $data->{stats2}->{ns}->{max} ? $data->{stats2}->{ns}->{max} : 0).' %</td></tr></tbody></table>';
        if($nscount) {
            $surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts2}->{ns},1),undef,'Percentage of N\'s (> 0%)','Percentage of N\'s per Read (1-100%)','# Sequences','',0);
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= '<br />'.&insert_image($png);
        }
    }
    if((exists $data->{counts}->{ns} && keys %{$data->{counts}->{ns}}) || (exists $data->{counts2} && exists $data->{counts2}->{ns} && keys %{$data->{counts2}->{ns}})) {
        $html .= '</div><hr>';
    }

    #tails
    if(exists $data->{tail} || exists $data->{tail2}) {
        $html .= '<div class="info-header"><span class="info-header-title">Poly-A/T Tails</span></div><div class="info-content">';
    }
    if(exists $data->{tail}) {
	my $tail5count = 0;
	foreach my $n (values %{$data->{counts}->{tail5}}) {
	    $tail5count += $n;
	}
	my $tail3count = 0;
	foreach my $n (values %{$data->{counts}->{tail3}}) {
	    $tail3count += $n;
	}
	if(exists $data->{pairedend} && $data->{pairedend}) {
            $html .= '<b>File 1</b><br />';
        }
	$html .= '<table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td class="info-table-type"></td><td class="info-table-value">5\'-end</td> <td class="info-table-value">3\'-end</td></tr> <tr><td class="info-table-type">Sequences with tail:</td><td class="info-table-value">'.($tail5count ? &addCommas($tail5count).' &nbsp;('.sprintf("%.2f",100/$data->{numseqs}*$tail5count).' %)' : 0).'</td> <td class="info-table-value">'.($tail3count ? &addCommas($tail3count).' &nbsp;('.sprintf("%.2f",100/$data->{numseqs}*$tail3count).' %)' : 0).'</td></tr> <tr><td class="info-table-type">Maximum tail length:</td> <td class="info-table-value">'.(exists $data->{stats}->{tail5}->{max} ? $data->{stats}->{tail5}->{max} : 0).'</td> <td class="info-table-value">'.(exists $data->{stats}->{tail3}->{max} ? $data->{stats}->{tail3}->{max} : 0).'</td></tr></tbody></table>';
        if($tail5count) {
            $surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts}->{tail5},1),undef,'Poly-A/T Tail Distribution (> 4bp)','5\' Tail Length in bp','# Sequences','',0,' bp');
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= '<br />'.&insert_image($png);
            if($tail3count) {
                $html .= '<br />';
            }
        }
        if($tail3count) {
            $surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts}->{tail3},1),undef,'Poly-A/T Tail Distribution (> 4bp)','3\' Tail Length in bp','# Sequences','',0,' bp');
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= '<br />'.&insert_image($png);
        }
    }
    if(exists $data->{pairedend} && $data->{pairedend} && exists $data->{tail2}) {
        my $tail5count = 0;
	foreach my $n (values %{$data->{counts2}->{tail5}}) {
	    $tail5count += $n;
	}
	my $tail3count = 0;
	foreach my $n (values %{$data->{counts2}->{tail3}}) {
	    $tail3count += $n;
	}
        $html .= '<br /><br /><br /><b>File 2</b><br />';
	$html .= '<table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td class="info-table-type"></td><td class="info-table-value">5\'-end</td> <td class="info-table-value">3\'-end</td></tr> <tr><td class="info-table-type">Sequences with tail:</td><td class="info-table-value">'.($tail5count ? &addCommas($tail5count).' &nbsp;('.sprintf("%.2f",100/$data->{numseqs2}*$tail5count).' %)' : 0).'</td> <td class="info-table-value">'.($tail3count ? &addCommas($tail3count).' &nbsp;('.sprintf("%.2f",100/$data->{numseqs2}*$tail3count).' %)' : 0).'</td></tr> <tr><td class="info-table-type">Maximum tail length:</td> <td class="info-table-value">'.(exists $data->{stats2}->{tail5}->{max} ? $data->{stats2}->{tail5}->{max} : 0).'</td> <td class="info-table-value">'.(exists $data->{stats2}->{tail3}->{max} ? $data->{stats2}->{tail3}->{max} : 0).'</td></tr></tbody></table>';
        if($tail5count) {
            $surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts2}->{tail5},1),undef,'Poly-A/T Tail Distribution (> 4bp)','5\' Tail Length in bp','# Sequences','',0,' bp');
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= '<br />'.&insert_image($png);
            if($tail3count) {
                $html .= '<br />';
            }
        }
        if($tail3count) {
            $surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{counts2}->{tail3},1),undef,'Poly-A/T Tail Distribution (> 4bp)','3\' Tail Length in bp','# Sequences','',0,' bp');
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= '<br />'.&insert_image($png);
        }
    }
    if(exists $data->{tail} || exists $data->{tail2}) {
        $html .= '</div><hr>';
    }
    

    #tag sequence check
    if(exists $data->{freqs} || exists $data->{freqs2}) {
        $html .= '<div class="info-header"><span class="info-header-title">Tag Sequence Check</span></div><div class="info-content">';
    }
    if(exists $data->{freqs}) {
	my $tagmidseq;
	if(exists $data->{tagmidseq}) {
	    $tagmidseq = $data->{tagmidseq};
	    $tagmidseq =~ s/\,/\<br \/\>/g;
	}
	if(exists $data->{pairedend} && $data->{pairedend}) {
            $html .= '<b>File 1</b><br />';
        }
	$html .= '<table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td class="info-table-type"></td><td class="info-table-value">5\'-end</td><td class="info-table-value">3\'-end</td></tr><tr><td class="info-table-type">Probability of tag sequence:</td><td class="info-table-value">'.(exists $data->{tagprob}->{5} ? $data->{tagprob}->{5}.' %' : '-').'</td><td class="info-table-value">'.(exists $data->{tagprob}->{3} ? $data->{tagprob}->{3}.' %' : '-').'</td></tr><tr><td class="info-table-type">GSMIDs or RLMIDs:</td><td class="info-table-value">'.(exists $data->{tagmidnum} ? ($data->{tagmidnum} == 0 ? 'none' : ($tagmidseq ? $tagmidseq : $data->{tagmidnum})) : '-').'</td><td class="info-table-value">&nbsp;</td></tr></tbody></table><br>';
	$html .= '<table border="0" cellspacing="0" cellpadding="0"><tr><td>'.&insert_image($FREQCHART_L,undef,undef,1).'</td>';
	foreach my $pos (sort {$a <=> $b} keys %{$data->{freqs}->{5}}) {
	    $html .= '<td valign="bottom" align="center">';
	    foreach my $base (qw(A C G T N)) {
		if($data->{freqs}->{5}->{$pos}->{$base}) {
		    $html .= &insert_image($BASE64_BASES->{$base},$data->{freqs}->{5}->{$pos}->{$base},14,1).'<br />';
		    #'<img height="'.$data->{freqs}->{5}->{pos}->{$base}.'px" border="0" src="'..'" alt="'.$base.'" width="14px" /><br />';
		}
	    }
	    $html .= &insert_image($MMCHART_B2,6,16,1).'</td>';
	}
	$html .= '<td align="center" valign="middle">&nbsp;...&nbsp;</td>';
	foreach my $pos (sort {$a <=> $b} keys %{$data->{freqs}->{3}}) {
	    $html .= '<td valign="bottom" align="center">';
	    foreach my $base (qw(A C G T N)) {
		if($data->{freqs}->{3}->{$pos}->{$base}) {
		    $html .= &insert_image($BASE64_BASES->{$base},$data->{freqs}->{3}->{$pos}->{$base},14,1).'<br />';
		}
	    }
	    $html .= &insert_image($MMCHART_B2,6,16,1).'</td>';
	}
	$html .= '</tr>';
	$html .= '<tr><td>&nbsp;</td>';
	foreach my $num (1,0,0,0,5,0,0,0,0,10,0,0,0,0,15,0,0,0,0,20,0,20,0,0,0,0,15,0,0,0,0,10,0,0,0,0,5,0,0,0,1) {
	    $html .= '<td valign="top" align="center" style="font-size: 10px;margin: 0;">'.($num ? $num : '').'&nbsp;</td>';
	}
	$html .= '</tr><tr><td align="left" valign="middle">&nbsp;</td><td align="center" valign="middle" colspan="41" class="pinfo"><b>Position from Sequence Ends</b></td></tr>';
	$html .= '</table>';
    }
    if(exists $data->{pairedend} && $data->{pairedend} && exists $data->{freqs2}) {
        $html .= '<br /><br /><br /><b>File 2</b><br />';
        $html .= '<table border="0" cellpadding="0" cellspacing="0"> <tbody><tr><td class="info-table-type"></td><td class="info-table-value">5\'-end</td><td class="info-table-value">3\'-end</td></tr><tr><td class="info-table-type">Probability of tag sequence:</td><td class="info-table-value">'.(exists $data->{tagprob2}->{5} ? $data->{tagprob2}->{5}.' %' : '-').'</td><td class="info-table-value">'.(exists $data->{tagprob2}->{3} ? $data->{tagprob2}->{3}.' %' : '-').'</td></tr></tbody></table><br>';
	$html .= '<table border="0" cellspacing="0" cellpadding="0"><tr><td>'.&insert_image($FREQCHART_L,undef,undef,1).'</td>';
	foreach my $pos (sort {$a <=> $b} keys %{$data->{freqs2}->{5}}) {
	    $html .= '<td valign="bottom" align="center">';
	    foreach my $base (qw(A C G T N)) {
		if($data->{freqs2}->{5}->{$pos}->{$base}) {
		    $html .= &insert_image($BASE64_BASES->{$base},$data->{freqs2}->{5}->{$pos}->{$base},14,1).'<br />';
		    #'<img height="'.$data->{freqs}->{5}->{pos}->{$base}.'px" border="0" src="'..'" alt="'.$base.'" width="14px" /><br />';
		}
	    }
	    $html .= &insert_image($MMCHART_B2,6,16,1).'</td>';
	}
	$html .= '<td align="center" valign="middle">&nbsp;...&nbsp;</td>';
	foreach my $pos (sort {$a <=> $b} keys %{$data->{freqs2}->{3}}) {
	    $html .= '<td valign="bottom" align="center">';
	    foreach my $base (qw(A C G T N)) {
		if($data->{freqs2}->{3}->{$pos}->{$base}) {
		    $html .= &insert_image($BASE64_BASES->{$base},$data->{freqs2}->{3}->{$pos}->{$base},14,1).'<br />';
		}
	    }
	    $html .= &insert_image($MMCHART_B2,6,16,1).'</td>';
	}
	$html .= '</tr>';
	$html .= '<tr><td>&nbsp;</td>';
	foreach my $num (1,0,0,0,5,0,0,0,0,10,0,0,0,0,15,0,0,0,0,20,0,20,0,0,0,0,15,0,0,0,0,10,0,0,0,0,5,0,0,0,1) {
	    $html .= '<td valign="top" align="center" style="font-size: 10px;margin: 0;">'.($num ? $num : '').'&nbsp;</td>';
	}
	$html .= '</tr><tr><td align="left" valign="middle">&nbsp;</td><td align="center" valign="middle" colspan="41" class="pinfo"><b>Position from Sequence Ends</b></td></tr>';
	$html .= '</table>';
    }
    if(exists $data->{freqs} || exists $data->{freqs2}) {
        $html .= '</div><hr>';
    }

    #Sequence duplicates
    if(exists $data->{dubslength} || exists $data->{dubscounts}) {
	$html .= '<div class="info-header"><span class="info-header-title">Sequence Duplication</span></div>';
    }
    my %dubs;
    if(exists $data->{dubscounts} && keys %{$data->{dubscounts}}) {
	my $exactonly = $data->{exactonly}||0;
	foreach my $n (keys %{$data->{dubscounts}}) {
	    foreach my $s (keys %{$data->{dubscounts}->{$n}}) {
		$dubs{$s}->{count} += $data->{dubscounts}->{$n}->{$s} * $n;
		$dubs{$s}->{max} = $n unless(exists $dubs{$s}->{max} && $dubs{$s}->{max} > $n);
		$dubs{all} += $data->{dubscounts}->{$n}->{$s} * $n;
	    }
	}
	$html .= '<div class="info-content"><table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td class="info-table-type"></td><td class="info-table-value"># Sequences</td> <td class="info-table-value">Max duplicates</td></tr><tr><td class="info-table-type">Exact duplicates:</td><td class="info-table-value">'.(exists $dubs{0}->{count} ? &addCommas($dubs{0}->{count}).' &nbsp;('.sprintf("%.2f",100/$data->{numseqs}*$dubs{0}->{count}).' %)' : 0).'</td><td class="info-table-value">'.($dubs{0}->{max}||0).'</td></tr><tr><td class="info-table-type">Exact duplicates with reverse complements:</td><td class="info-table-value">'.(exists $dubs{3}->{count} ? &addCommas($dubs{3}->{count}).' &nbsp;('.sprintf("%.2f",100/$data->{numseqs}*$dubs{3}->{count}).' %)' : 0).'</td> <td class="info-table-value">'.($dubs{3}->{max}||0).'</td></tr>';
        unless($exactonly) {
            $html .= '<tr><td class="info-table-type">5\' duplicates</td><td class="info-table-value">'.(exists $dubs{1}->{count} ? &addCommas($dubs{1}->{count}).' &nbsp;('.sprintf("%.2f",100/$data->{numseqs}*$dubs{1}->{count}).' %)' : 0).'</td> <td class="info-table-value">'.($dubs{1}->{max}||0).'</td></tr><tr><td class="info-table-type">3\' duplicates</td><td class="info-table-value">'.(exists $dubs{2}->{count} ? &addCommas($dubs{2}->{count}).' &nbsp;('.sprintf("%.2f",100/$data->{numseqs}*$dubs{2}->{count}).' %)' : 0).'</td> <td class="info-table-value">'.($dubs{2}->{max}||0).'</td></tr><tr><td class="info-table-type">5\'/3\' duplicates with reverse complements</td><td class="info-table-value">'.(exists $dubs{4}->{count} ? &addCommas($dubs{4}->{count}).' &nbsp;('.sprintf("%.2f",100/$data->{numseqs}*$dubs{4}->{count}).' %)' : 0).'</td> <td class="info-table-value">'.($dubs{4}->{max}||0).'</td></tr>';
        }
        $html .= '<tr><td class="info-table-type">Total:</td><td class="info-table-value">'.(exists $dubs{all} ? &addCommas($dubs{all}).' &nbsp;('.sprintf("%.2f",100/$data->{numseqs}*$dubs{all}).' %)' : 0).'</td><td class="info-table-value">-</td></tr></tbody></table>';
    }
    if(exists $dubs{all} && $dubs{all}) {
        if(exists $data->{dubslength} && keys %{$data->{dubslength}}) {
            $surface = &createStackBarPlot(&convertOdToStackBinMatrix($data->{dubslength},5,1),'Sequence duplication level','Read Length in bp','Number of duplicates','',0,' bp');
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= '<br />'.&insert_image($png);
        }
        if(exists $data->{dubscounts} && keys %{$data->{dubscounts}}) {
            $surface = &createStackBarPlot(&convertOdToStackBinMatrix($data->{dubscounts},5,1,100),'Sequence duplication level','Number of duplicates','Number of sequences','',0);
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= '<br /><br />' if(exists $data->{dubslength});
            $html .= &insert_image($png);
            my %dubsmax;
            my $count = 1;
            foreach my $n (sort {$b <=> $a} keys %{$data->{dubscounts}}) {
                foreach my $s (keys %{$data->{dubscounts}->{$n}}) {
                    foreach my $i (1..$data->{dubscounts}->{$n}->{$s}) {
                        $dubsmax{$count++}->{$s} = $n;
                        last unless($count <= 100);
                    }
                    last unless($count <= 100);
                }
                last unless($count <= 100);
            }
            $surface = &createStackBarPlot(&convertOdToStackBinMatrix(\%dubsmax,5,1,100),'Sequence duplication level','Sequence','Number of duplicates','',0);
            $png = '';
            $surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
            $html .= '<br /><br />' if(exists $data->{dubslength});
            $html .= &insert_image($png);
        }
    }
    if(exists $data->{dubslength} || exists $data->{dubscounts}) {
	$html .= '</div><hr>';
    }

    #Sequence complexity
    if(exists $data->{compldust} || exists $data->{complentropy}) {
	$html .= '<div class="info-header"><span class="info-header-title">Sequence Complexity</span></div>';
	if(exists $data->{complvals}) {
	    my $complseq;
	    foreach my $d (keys %{$data->{complvals}}) {
		foreach my $m ('minseq','maxseq') {
		    $complseq = $data->{complvals}->{$d}->{$m};
		    $complseq = substr($complseq,0,797).'...' if(length($complseq) > 800);
		    $complseq =~ s/(.{60})/$1\<br \/\>/g;
		    $data->{complvals}->{$d}->{$m} = $complseq;
		}
	    }
	}
	$html .= '<div class="info-content"><table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td class="info-table-type"></td><td class="info-table-value">Value</td><td class="info-table-value">Sequence</td></tr><tr><td class="info-table-type">Minimum DUST score:</td><td class="info-table-value">'.(exists $data->{complvals}->{dust}->{minval} ? $data->{complvals}->{dust}->{minval} : '-').'</td><td class="info-table-value sequencetext">'.(exists $data->{complvals}->{dust}->{minseq} ? $data->{complvals}->{dust}->{minseq} : '').'</td></tr><tr><td class="info-table-type">Maximum DUST score:</td><td class="info-table-value">'.(exists $data->{complvals}->{dust}->{maxval} ? $data->{complvals}->{dust}->{maxval} : '').'</td><td class="info-table-value sequencetext">'.(exists $data->{complvals}->{dust}->{maxseq} ? $data->{complvals}->{dust}->{maxseq} : '').'</td></tr><tr><td class="info-table-type">Minimum Entropy value:</td><td class="info-table-value">'.(exists $data->{complvals}->{entropy}->{minval} ? $data->{complvals}->{entropy}->{minval} : '').'</td><td class="info-table-value sequencetext">'.(exists $data->{complvals}->{entropy}->{minseq} ? $data->{complvals}->{entropy}->{minseq} : '').'</td></tr><tr><td class="info-table-type">Maximum Entropy value:</td><td class="info-table-value">'.(exists $data->{complvals}->{entropy}->{maxval} ? $data->{complvals}->{entropy}->{maxval} : '').'</td><td class="info-table-value sequencetext">'.(exists $data->{complvals}->{entropy}->{maxseq} ? $data->{complvals}->{entropy}->{maxseq} : '').'</td></tr> </tbody></table><br />';
    }
    if(exists $data->{compldust}) {
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{compldust},0),undef,'Sequence complexity distribution','Mean sequence complexity (DUST scores)','Number of sequences','',1);
	$png = '';
	$surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
	$html .= &insert_image($png);
    }
    if(exists $data->{complentropy}) {
	$surface = &createAnnotBarPlot(&convertOdToBinMatrix($data->{complentropy},0),undef,'Sequence complexity distribution','Mean sequence complexity (Entropy values)','Number of sequences','',1);
	$png = '';
	$surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
	$html .= '<br /><br />' if(exists $data->{compldust});
	$html .= &insert_image($png);
    }
    if(exists $data->{compldust} || exists $data->{complentropy}) {
	$html .= '</div><hr>';
    }

    #Dinucleotide odd ratio PCA - microbial/viral
    if(exists $data->{dinucodds} && keys %{$data->{dinucodds}}) {
	$html .= '<div class="info-header"><span class="info-header-title">Dinucleotide Odds Ratios</span></div>';
	$html .= '<div class="info-content"><table border="0" cellpadding="0" cellspacing="0"><tbody><tr><td class="info-table-type">&nbsp;</td>';
	foreach my $d (map {join("/",(m/../g ))} sort keys %{$data->{dinucodds}}) {
	    $html .= '<td class="info-table-value">'.$d.'</td>';
	}
	$html .= '</tr><tr><td class="info-table-type">Odds ratio</td>';
	foreach my $d (map {sprintf("%.4f",$data->{dinucodds}->{$_})} sort keys %{$data->{dinucodds}}) {
	    $html .= '<td class="info-table-value">'.$d.'</td>';
	}
	$html .= '</tr></tbody></table><br />';
	my @new = map {$data->{dinucodds}->{$_}} sort keys %{$data->{dinucodds}};
        $surface = &createOddsRatioPlot($data->{dinucodds},'Odds ratios','Dinucleotide','Odds ratio','');
	$png = '';
	$surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
	$html .= &insert_image($png);
	#$surface = &createPCAPlot(&convertToPCAValues(\@new,'m'),'PCA','1st Principal Component Score','2nd Principal Component Score','');
	#$png = '';
	#$surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
	#$html .= '<br /><br />';
	#$html .= &insert_image($png);
	#$surface = &createPCAPlot(&convertToPCAValues(\@new,'v'),'PCA','1st Principal Component Score','2nd Principal Component Score','');
	#$png = '';
	#$surface->write_to_png_stream(sub { my ($closure, $data) = @_; $png .= $data; });
	#$html .= '<br /><br />';
	#$html .= &insert_image($png);
	$html .= '</div>';
    }

    $html .= '</div>';
    $html .= &footer();

    #write html to file
    $file = &getFileName('.html');
    open(FH, ">$file") or &printError("Can't open file ".$file.": $!");
    print FH $html;
    close(FH);
    &printLog("Done with HTML data");
}

sub insert_image {
  my ($data, $height, $width, $noencode) = @_;
  my $content .= '<img border="0" '.($height ? 'height="'.$height.'"' : '').($width ? 'width="'.$width.'"' : '').' src="'.($noencode ? "data:image/png;base64,".$data : &inline_image($data)).'" />'."\n";
  return $content;
}

sub inline_image {
    return "data:image/png;base64,".MIME::Base64::encode_base64($_[0]);
}

sub convertIntToString {
    my $int = shift;
    $int =~ s/(.{2})/chr(hex($1))/eg;
    return $int;
}