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1 Project Details1: PeptideShaker Version: 0.37.4-beta
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2 2: Date: Sun Feb 15 09:12:50 CET 2015
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3 3: Experiment: Galaxy_Experiment_2015021509121423987968
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4 4: Sample: Sample_2015021509121423987968
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5 5: Replicate Number: 1
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6 6: Identification Algorithms: X!Tandem and MS-GF+
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7 Database Search Parameters
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8
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9 1: Precursor Accuracy Unit: ppm
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10 2: Precursor Ion m/z Tolerance: 100.0
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11 3: Fragment Ion m/z Tolerance: 0.5
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12 4: Enzyme: Trypsin
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13 5: Number of Missed Cleavages: Not implemented
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14 6: Database: input_database.fasta
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15 7: Forward Ion: b
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16 8: Rewind Ion: y
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17 9: Fixed Modifications: carbamidomethyl c
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18 10: Variable Modifications: oxidation of m, acetylation of protein n-term, pyro-cmc, pyro-glu from n-term e, pyro-glu from n-term q
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19 11: Refinement Variable Modifications:
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20 12: Refinement Fixed Modifications:
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21 Input Filters
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22
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23 1: Minimal Peptide Length: 1
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24 2: Maximal Peptide Length: 30
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25 3: Precursor m/z Tolerance: 10.0
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26 4: Precursor m/z Tolerance Unit: Yes
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27 5: Unrecognized Modifications Discarded: No
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28 Validation Summary
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29
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30 1: #Validated Proteins: 0.0
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31 2: Protein Total: 0.0
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32 3Protein FDR Limit: 1.0 %
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33 4: Protein FNR Limit: 0.0 %
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34 5: Protein Confidence Limit: -1.0 %
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35 6: Protein PEP Limit: 101.0 %
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36 7: Protein Confidence Accuracy: 0.0 %
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37 8: #Validated Peptides: 0.0
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38 9: Peptide Total: 0.0
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39 10: Peptide FDR Limit: 1.0 %
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40 11: Peptide FNR Limit: 0.0 %
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41 12: Peptide Confidence Limit: -1.0 %
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42 13: Peptide PEP Limit: 101.0 %
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43 14: Peptide Confidence Accuracy: 0.0 %
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44 15: #Validated PSM: 0.0
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45 16: PSM Total: 0.0
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46 17: PSM FDR Limit: 1.0 %
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47 18: PSM FNR Limit: 0.0 %
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48 19: PSM Confidence Limit: -1.0 %
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49 20: PSM PEP Limit: 101.0 %
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50 21: PSM Confidence Accuracy: 0.0 %
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51 Postranslational Modification Scoring Settings
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52
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53 1: A-score: No
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54 2: Accounting for Neutral Losses: No
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55 3: False Location Rate: 1.0
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56 Spectrum Counting Parameters
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57
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58 1: Method: NSAF
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59 2: Validated Matches Only: Yes
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60 Annotation Settings
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61
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62 1: Intensity Limit: 0.75
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63 2: Automatic Annotation: Yes
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64 3: Selected Ions: b, y
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65 4: Neutral Losses: NH3, H2O
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66 5: Neutral Losses Sequence Dependence: Yes
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67 6: Selected Charges: 1, 2
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68 7: Fragment Ion m/z Tolerance: 0.5
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