Mercurial > repos > bgruening > deeptools_plot_heatmap

annotate plotHeatmap.xml @ 27:b3016f2736d2 draft

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planemo upload for repository https://github.com/fidelram/deepTools/tree/master/galaxy/wrapper/ commit 999ec5d9b5882702497d8117a7d26536a67e19ca
author bgruening
date Tue, 24 Jan 2017 04:00:11 -0500
parents 671b777fde9a
children 6d5a88e9c5ae
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1 <tool id="deeptools_plot_heatmap" name="plotHeatmap" version="@WRAPPER_VERSION@.0">
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2 <description>creates a heatmap for score distributions across genomic regions</description>
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3 <macros>
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4 <token name="@BINARY@">plotHeatmap</token>
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5 <import>deepTools_macros.xml</import>
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6 </macros>
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7 <expand macro="requirements"/>
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8 <command>
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9 <![CDATA[
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10 @BINARY@
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11 --matrixFile "$matrixFile"
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12 --outFileName "$outFileName"
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14 #if $output.showOutputSettings == "yes"
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15 --plotFileFormat "$output.outFileFormat"
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17 #if $outFileNameMatrix:
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18 --outFileNameMatrix "$outFileNameMatrix"
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19 #end if
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21 #if $outFileSortedRegions:
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22 --outFileSortedRegions "$outFileSortedRegions"
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23 #end if
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25 #if $output.dpi:
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26 --dpi "$output.dpi"
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27 #end if
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28 #else
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29 --plotFileFormat 'png'
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30 #end if
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32 #if $advancedOpt.showAdvancedOpt == "yes"
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33 #if $advancedOpt.sortRegions:
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34 --sortRegions '$advancedOpt.sortRegions'
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35 #end if
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37 #if $advancedOpt.sortUsing:
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38 --sortUsing '$advancedOpt.sortUsing'
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39 #end if
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41 #if $advancedOpt.averageTypeSummaryPlot:
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42 --averageTypeSummaryPlot '$advancedOpt.averageTypeSummaryPlot'
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43 #end if
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45 #if str($advancedOpt.missingDataColor.value) != "None":
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46 --missingDataColor '$advancedOpt.missingDataColor'
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47 #end if
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49 #if str($advancedOpt.colorMap).strip() != "":
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50 --colorMap #echo " ".join(str($advancedOpt.colorMap).strip().split(","))#
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51 #end if
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52 --alpha '$advancedOpt.alpha'
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53 #if str($advancedOpt.colorList).strip() != "":
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54 --colorList $advancedOpt.colorList
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55 #end if
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57 #if str($advancedOpt.zMin).strip() != "":
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58 --zMin $advancedOpt.zMin
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59 #end if
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60 #if str($advancedOpt.zMax).strip() != "":
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61 --zMax $advancedOpt.zMax
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62 #end if
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64 #if str($advancedOpt.yMin).strip() != "":
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65 --yMin $advancedOpt.yMin
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66 #end if
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67 #if $advancedOpt.yMax:
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68 --yMax $advancedOpt.yMax
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69 #end if
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71 --xAxisLabel '$advancedOpt.xAxisLabel'
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72 --yAxisLabel '$advancedOpt.yAxisLabel'
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74 --heatmapWidth $advancedOpt.heatmapWidth
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75 --heatmapHeight $advancedOpt.heatmapHeight
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77 --whatToShow '$advancedOpt.whatToShow'
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79 --startLabel '$advancedOpt.startLabel'
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80 --endLabel '$advancedOpt.endLabel'
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82 --refPointLabel '$advancedOpt.referencePointLabel'
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84 #if $advancedOpt.samplesLabel and str($advancedOpt.samplesLabel).strip() != "":
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85 --samplesLabel $advancedOpt.samplesLabel
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86 #end if
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88 #if $advancedOpt.regionsLabel and str($advancedOpt.regionsLabel).strip() != "":
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89 --regionsLabel $advancedOpt.regionsLabel
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90 #end if
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92 #if $advancedOpt.plotTitle and str($advancedOpt.plotTitle.value) != "":
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93 --plotTitle '$advancedOpt.plotTitle'
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94 #end if
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95
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96 $advancedOpt.perGroup
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97
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98 @KMEANS_CLUSTERING@
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99
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100 #end if
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101 ]]>
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102 </command>
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103 <inputs>
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104 <param argument="matrixFile" format="deeptools_compute_matrix_archive" type="data"
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105 label="Matrix file from the computeMatrix tool" help=""/>
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106
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107 <expand macro="input_graphic_output_settings">
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108 <expand macro="input_image_file_format" />
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109 <expand macro="output_dpi" />
14
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110 <expand macro="input_save_matrix_values" />
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111 </expand>
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112
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113 <conditional name="advancedOpt" >
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114 <param name="showAdvancedOpt" type="select" label="Show advanced options" >
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115 <option value="no" selected="true">no</option>
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116 <option value="yes">yes</option>
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117 </param>
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118 <when value="no" />
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119 <when value="yes">
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120 <expand macro="sortRegions" />
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121 <expand macro="sortUsing" />
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122 <param argument="--averageTypeSummaryPlot" type="select"
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123 label="Type of statistic that should be plotted in the summary image above the heatmap"
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124 help="">
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125 <option value="mean" selected="true">mean</option>
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126 <option value="median">median</option>
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127 <option value="min">min</option>
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128 <option value="max">max</option>
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129 <option value="sum">sum</option>
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130 <option value="std">std</option>
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131 </param>
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132
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133 <param argument="--missingDataColor" type="text" value="black" optional="true" label="Missing data color"
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134 help="If 'Represent missing data as zero' is not set, such cases will be colored in black by default.
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135 By using this parameter a different color can be set. A value between 0 and 1 will be used for a gray scale (black is 0).
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136 Also color names can be used, see a list here: http://packages.python.org/ete2/reference/reference_svgcolors.html.
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137 Alternatively colors can be specified using the #rrggbb notation." />
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138
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139 <expand macro="colorMap" />
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140 <param argument="--alpha" type="float" value="1.0" min="0.0"
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141 max="1.0" optional="True"
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142 label="Alpha channel (transparency)"
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143 help="The alpha channel (transparency) to use for the heatmaps. The default is 1.0 and values must
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144 be between 0 and 1. A value of 0.0 would be fully transparent." />
14
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145
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146 <param argument="--colorList" type="text" value="" size="50" optional="True"
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147 label="List of colors for each heatmap. Separate lists by spaces and the colors in the list by comas"
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148 help="The color of the heatmaps can be specified as a list of colors separated by comas with
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149 not space in between. For example: white,blue white,green will set a color map from white
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150 to blue for the first heatmap and for white to green for the next heatmap."/>
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151 <expand macro="zMin_zMax" />
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152 <param argument="--yMin" type="float" value="" size="3" optional="True"
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153 label="Minimum value for the Y-axis of the summary plot. Leave empty for automatic values" help=""/>
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154 <param argument="--yMax" type="float" value="" size="3" optional="True"
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155 label="Maximum value for Y-axis of the summary plot. Leave empty for automatic values" help=""/>
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156 <param argument="--xAxisLabel" type="text" value="distance from TSS (bp)" size="200"
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157 label="The x-axis label" help="" />
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158 <param argument="--yAxisLabel" type="text" value="genes" size="30"
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159 label="The y-axis label for the top panel" help="" />
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160
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161 <param argument="--heatmapWidth" type="float" value="7.5" min="1" max="100"
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162 label="Heatmap width in cm" help="The minimum value is 1 and the maximum is 100."/>
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163 <param argument="--heatmapHeight" type="float" value="25" min="3" max="100"
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164 label="Heatmap height in cm" help="The minimum value is 3 and the maximum is 100."/>
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165
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166 <param argument="--whatToShow" type="select" label="What to show"
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167 help ="The default is to include a summary or profile plot on top of the heatmap and a heatmap colorbar.">
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168 <option value="plot, heatmap and colorbar" selected="true">summary plot, heatmap and colorbar</option>
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169 <option value="plot and heatmap">summary plot and heatmap (no colorbar)</option>
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170 <option value="heatmap and colorbar">heatmap and colorbar</option>
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171 </param>
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172
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173 <param argument="--startLabel" type="text" value="TSS" size="10"
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174 label="Label for the region start"
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175 help ="Only for scale-regions mode. Label shown in the plot for the start of the region. Default is TSS (transcription start site), but could be changed to anything, e.g. &quot;peak start&quot;." />
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176 <param argument="--endLabel" type="text" value="TES" size="10"
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177 label="Label for the region end"
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178 help="Only for scale-regions mode. Label shown in the plot for the region end. Default is TES (transcription end site)."/>
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179
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180 <param argument="--referencePointLabel" type="text" value="TSS" size="10"
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181 label="Reference point label"
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182 help ="Label shown in the plot for the reference-point. Default is the same as the reference point selected (e.g. TSS), but could be anything, e.g. &quot;peak start&quot; etc." />
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183 <param argument="--samplesLabel" type="text" size="30"
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184 label="Labels for the samples (each bigwig) plotted"
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185 help="The default is to use the file name of the sample. The sample labels should be separated by
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186 spaces and quoted if a label itself contains a space E.g. label-1 &quot;label 2&quot;">
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187 <sanitizer>
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188 <valid initial="string.printable">
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189 </valid>
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190 </sanitizer>
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191 </param>
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192 <param argument="--regionsLabel" type="text" size="30"
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193 label="Labels for the regions plotted in the heatmap"
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194 help="If more than one region is being plotted a list of labels separated by space is required.
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195 If a label itself contains a space, then quotes are needed.
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196 For example, label_1 &quot;label 2&quot;" />
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197
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198 <expand macro="plotTitle" />
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199 <param argument="--perGroup" type="boolean" truevalue="--perGroup" falsevalue=""
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200 label="Make one plot per group of regions"
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201 help="The default is to make one plot per bigWig file, i.e., all samples next to each other. Choosing this option will make one plot per group of regions."/>
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202
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203 <expand macro="kmeans_clustering" />
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204 </when>
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205 </conditional>
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206 </inputs>
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207 <outputs>
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208 <expand macro="output_image_file_format" />
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209 <expand macro="output_graphic_outputs" />
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210 <expand macro="output_save_matrix_values" />
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211 </outputs>
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212 <tests>
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213 <test>
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214 <param name="matrixFile" value="computeMatrix_result1.gz" ftype="deeptools_compute_matrix_archive" />
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215 <output name="outFileName" file="heatmapper_result1.png" ftype="png" compare="sim_size" delta="4000" />
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216 </test>
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217 <test>
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218 <param name="matrixFile" value="computeMatrix_result1.gz" ftype="deeptools_compute_matrix_archive" />
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219 <param name="showAdvancedOpt" value="yes" />
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220 <param name="whatToShow" value="heatmap and colorbar" />
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221 <output name="outFileName" file="heatmapper_result2.png" ftype="png" compare="sim_size" delta="4000" />
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222 </test>
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223 </tests>
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224 <help>
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225 <![CDATA[
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226
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227 What it does
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228 --------------
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229
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230 ``plotHeatmap`` visualizes scores associated with genomic regions, for example ChIP enrichment values around the TSS of genes.
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231 Like ``plotProfile``, it requires that computeMatrix was run first to calculate the values.
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232
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233 We have implemented a number of optional parameters to optimize the visual output and we encourage you to play around with the min/max values
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234 displayed in the heatmap as well as with the different coloring options.
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235
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236 **TIP:** *If your data is rather sparse and the resulting heatmap is too black, change the "Missing data color" to white (via "Advanced options").*
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237
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238 The most powerful option is probably the **k-means/hierarchical clustering** where you can sort your regions into groups of regions with similar score distributions.
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239
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240 **NOTE:** *The clustering will only work if you supplied a single BED file to computeMatrix. plotHeatmap cannot cluster regions within pre-defined groups. Moreover, k-means will be much faster than hierarchical clustering.*
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241
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242 Output
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243 --------------
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244
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245 This is a heatmap based on two bigWig files generated with default settings and k-means clustering.
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246
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247 .. image:: $PATH_TO_IMAGES/plotHeatmap_example.png
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248 :width: 600
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249 :height: 694
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250
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251 .. image:: $PATH_TO_IMAGES/plotHeatmap_example02.png
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252 :width: 600
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253 :height: 694
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254
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255 In addition to the image, ``plotHeatmap`` can also generate the values underlying both the heatmap.
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256
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257 See the following table for the optional output options:
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258
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259 +-----------------------------------+--------------------+-----------------+-----------------+
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260 | **optional output type** | **computeMatrix** | **plotHeatmap** | **plotProfile** |
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261 +-----------------------------------+--------------------+-----------------+-----------------+
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262 | values underlying the heatmap | yes | yes | no |
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263 +-----------------------------------+--------------------+-----------------+-----------------+
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264 | values underlying the profile | no | no | yes |
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265 +-----------------------------------+--------------------+-----------------+-----------------+
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266 | sorted and/or filtered regions | yes | yes | yes |
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267 +-----------------------------------+--------------------+-----------------+-----------------+
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268
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269 **More examples** can be found in our `Gallery <http://deeptools.readthedocs.org/en/latest/content/example_gallery.html#normalized-chip-seq-signals-and-peak-regions>`_.
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270
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271 -----
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273 @REFERENCES@
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274 ]]>
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275 </help>
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276 <expand macro="citations" />
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277 </tool>