Mercurial > repos > bebatut > convert_extract_sequence_file
changeset 0:b4cf778be846 draft
planemo upload commit 23ef4b1699065b4f6200c58328bfecfb33dd7fd1-dirty
| author | bebatut |
|---|---|
| date | Tue, 26 Apr 2016 08:17:42 -0400 |
| parents | |
| children | d7747e6f329f |
| files | convert_extract_sequence_file.py convert_extract_sequence_file.xml test-data/extracted_quality_illumina_1_3_fastq_output.qual test-data/extracted_sequences_illumina_1_3_fastq_output.fasta test-data/information_lenght_fasta_output.txt test-data/input_sequence_file.fasta test-data/input_sequence_file.fastq test-data/report_illumina_1_3_fastq_output.txt test-data/report_length_fasta_output.txt |
| diffstat | 9 files changed, 1374 insertions(+), 0 deletions(-) [+] |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/convert_extract_sequence_file.py Tue Apr 26 08:17:42 2016 -0400 @@ -0,0 +1,414 @@ +#!/usr/bin/python +# -*- coding: utf-8 -*- + +import sys +import os +import argparse +import copy +import operator + +FASTA_FILE_LAST_POS = None + +################# +# Parse methods # +################# +def text_end_of_file(row): + if row == '': + return True + else: + return False + +def get_new_line(input_file, generate_error = True): + row = input_file.readline() + if text_end_of_file(row): + if generate_error : + string = os.path.basename(__file__) + ': ' + string += ' unexpected end of file' + raise ValueError(string) + else : + return None + else: + return row[:-1] + +def next_fasta_record(input_file): + global FASTA_FILE_LAST_POS + if FASTA_FILE_LAST_POS != None: + input_file.seek(FASTA_FILE_LAST_POS) + else: + FASTA_FILE_LAST_POS = input_file.tell() + + id_line = get_new_line(input_file, generate_error = False) + if id_line == None: + return None + split_line = id_line[1:].split(' ') + seq_id = split_line[0] + description = ' '.join(split_line[1:]) + new_line = get_new_line(input_file, generate_error = False) + seq = '' + while new_line != None: + if new_line[0] != '>': + seq += new_line + FASTA_FILE_LAST_POS = input_file.tell() + new_line = get_new_line(input_file, generate_error = False) + else: + new_line = None + return SeqRecord(seq_id, seq, description) + +def next_fastq_record(input_file): + id_line = get_new_line(input_file, generate_error = False) + if id_line == None: + return None + if id_line[0] != '@': + string = os.path.basename(__file__) + ': ' + string += ' issue in fastq file' + raise ValueError(string) + split_line = id_line[1:].split(' ') + seq_id = split_line[0] + description = ' '.join(split_line[1:]) + seq = get_new_line(input_file) + spacer = get_new_line(input_file) + quals = get_new_line(input_file) + return SeqRecord(seq_id, seq, description, quals) + +def next_record(input_file, file_format): + if file_format == 'fasta': + return next_fasta_record(input_file) + elif file_format == 'fastq': + return next_fastq_record(input_file) + else: + string = os.path.basename(__file__) + ': ' + string += file_format + ' is not managed' + raise ValueError(string) + +def write_fasta_record(record, output_sequence_file): + output_sequence_file.write('>' + record.get_id() + ' ' + + record.get_description() + '\n') + seq = record.get_sequence() + split_seq = [seq[i:i+60] for i in xrange(0,len(seq),60)] + for split in split_seq: + output_sequence_file.write(split + '\n') + +def format_qual_value(qual_score, sliding_value, authorized_range, qual_format): + ascii_value = ord(qual_score) + score = ascii_value-sliding_value + if score < authorized_range[0] or score > authorized_range[1]: + string = os.path.basename(__file__) + ': wrong score (' + string += str(score) + ') with quality format (' + string += qual_format + raise ValueError(string) + return score + +def format_qual_string(qual_string, qual_format): + if qual_format == 'sanger': + return format_qual_value(qual_string, 33 ,[0,40], qual_format) + elif qual_format == "solexa": + return format_qual_value(qual_string, 64 ,[-5,40], qual_format) + elif qual_format == "illumina_1_3": + return format_qual_value(qual_string, 33 ,[0,40], qual_format) + elif qual_format == "illumina_1_5": + return format_qual_value(qual_string, 33 ,[3,40], qual_format) + elif qual_format == "illumina_1_8": + return format_qual_value(qual_string, 33 ,[0,41], qual_format) + else: + string = os.path.basename(__file__) + ': quality format (' + string += qual_format + ') is not managed' + raise ValueError(string) + +def write_qual_record(record, output_qual_file, qual_format): + output_qual_file.write('>' + record.get_id() + ' ' + + record.get_description() + '\n') + qual = record.get_quality() + qual = [str(format_qual_string(qual_str,qual_format)) for qual_str in qual] + split_seq = [qual[i:i+60] for i in xrange(0,len(qual),60)] + for split in split_seq: + output_qual_file.write(' '.join(split) + '\n') + +def write_fastq_record(record, output_sequence_file): + output_sequence_file.write('@' + record.get_id() + ' ' + + record.get_description() + '\n') + output_sequence_file.write(record.get_sequence() + '\n') + output_sequence_file.write('+\n') + output_sequence_file.write(record.get_quality() + '\n') + +def write_information(record, output_file_formats, output_sequence_file, + output_qual_file, qual_format): + if "fasta" in output_file_formats: + write_fasta_record(record, output_sequence_file) + if "qual" in output_file_formats: + write_qual_record(record, output_qual_file, qual_format) + if "fastq" in output_file_formats: + write_fastq_record(record, output_sequence_file) + +def fast_test_element_in_list(element,list_to_test): + to_continue = True + i = 0 + while to_continue: + if i == len(list_to_test) or list_to_test[i] >= element: + to_continue = False + else: + i += 1 + + found = False + if i < len(list_to_test): + if list_to_test[i] == element: + found = True + + return found + +######################### +# Constraint definition # +######################### +constraints = { + 'equal': operator.eq, + 'different': operator.ne, + 'lower': operator.le, + 'strictly_lower': operator.lt, + 'greater': operator.ge, + 'strictly_greater': operator.gt, + 'in': operator.contains, + 'not_in': 'in' +} + +extractable_information = { + 'id': str, + 'length': int, + 'description': str +} + +########### +# Classes # +########### +class SeqRecord: + + def __init__(self, seq_id, sequence, description, quality = ""): + self.id = seq_id + self.sequence = sequence + self.quality = quality + self.description = description + self.length = len(self.sequence) + + # Getters + def get_id(self): + return self.id + + def get_sequence(self): + return self.sequence + + def get_quality(self): + return self.quality + + def get_length(self): + return self.length + + def get_description(self): + return self.description + + def get(self, category): + if category == 'id': + return self.get_id() + elif category == 'length': + return self.get_length() + elif category == 'description': + return self.get_description() + else: + string = os.path.basename(__file__) + ': ' + string += category + ' can not be extracted from SeqRecord' + raise ValueError(string) + + # Other functions + def extract_information(self,to_extract): + extracted_info = [] + for info_to_extract in to_extract: + extracted_info.append(self.get(info_to_extract)) + return extracted_info + + def test_conservation(self, constraints): + to_conserve = True + for constrained_info in constraints: + record_value = self.get(constrained_info) + for constraint in constraints[constrained_info]: + to_conserve &= constraint.test_constraint(record_value) + return to_conserve + +class Records: + + def __init__(self, input_filepath, file_format, constraints): + self.records = [] + self.conserved_records = [] + with open(input_filepath, 'r') as input_file: + to_continue = True + while to_continue: + record = next_record(input_file, file_format) + if record != None: + self.records.append(record) + to_conserve = record.test_conservation(constraints) + if to_conserve: + self.conserved_records.append(copy.copy(record)) + else: + to_continue = False + + # Getters + def get_records(self): + return copy.copy(self.records) + + def get_record_nb(self): + return len(self.records) + + def get_conserved_records(self): + return copy.copy(self.conserved_records) + + def get_conserved_record_nb(self): + return len(self.conserved_records) + + # Other functions + def save_conserved_records(self,args): + if args.custom_extraction_type == 'True': + to_extract = args.to_extract[1:-1].split(',') + with open(args.output_information, 'w') as output_information_file: + output_information_file.write('\t'.join(to_extract) + '\n') + for record in self.conserved_records: + extracted_info = record.extract_information(to_extract) + string_info = [str(info) for info in extracted_info] + string = '\t'.join(string_info) + output_information_file.write(string + '\n') + else: + qual_format = None + if args.format == 'fasta': + output_file_formats = ['fasta'] + elif args.format == 'fastq': + if args.split == 'True': + output_file_formats = ['fasta','qual'] + qual_format = args.quality_format + else: + output_file_formats = ['fastq'] + + with open(args.output_sequence,'w') as output_sequence_file: + if "qual" in output_file_formats: + output_qual_file = open(args.output_quality, 'w') + else: + output_qual_file = None + for record in self.conserved_records: + write_information(record, output_file_formats, + output_sequence_file, output_qual_file, qual_format) + if "qual" in output_file_formats: + output_qual_file.close() + +class Constraint: + + def __init__(self, constraint_type, value, constrained_information): + if not constraints.has_key(constraint_type): + string = os.path.basename(__file__) + ': ' + string += constraint_type + ' is not a correct type of constraint' + raise ValueError(string) + self.raw_constraint_type = constraint_type + self.type = constraints[constraint_type] + + value_format = extractable_information[constrained_information] + if self.raw_constraint_type in ['in', 'not_in']: + self.values = [] + with open(value, 'r') as value_file: + for row in value_file.readlines(): + value = row[:-1] + self.values.append(value_format(value)) + else: + self.values = [value_format(value)] + self.values.sort() + + def get_raw_constraint_type(self): + return self.raw_constraint_type + + def get_type(self): + return self.type + + def get_values(self): + return self.values + + def test_constraint(self, similarity_info_value): + to_conserve = True + if self.raw_constraint_type == 'in': + to_conserve &= fast_test_element_in_list(similarity_info_value, + self.values) + elif self.raw_constraint_type == 'not_in': + to_conserve &= (not fast_test_element_in_list(similarity_info_value, + self.values)) + else: + to_conserve &= self.type(similarity_info_value, self.values[0]) + return to_conserve + +################ +# Misc methods # +################ +def test_input_filepath(input_filepath, tool, file_format): + if not os.path.exists(input_filepath): + string = os.path.basename(__file__) + ': ' + string += input_filepath + ' does not exist' + raise ValueError(string) + +def format_constraints(constraints): + formatted_constraints = {} + if constraints != None: + for constr in constraints: + split_constraint = constr.split(': ') + constrained_information = split_constraint[0] + constraint = Constraint(split_constraint[1], split_constraint[2], + constrained_information) + formatted_constraints.setdefault(constrained_information,[]).append( + constraint) + return formatted_constraints + +def convert_extract_sequence_file(args): + input_filepath = args.input + file_format = args.format + constraints = args.constraint + formatted_constraints = format_constraints(constraints) + + records = Records(input_filepath, file_format, formatted_constraints) + records.save_conserved_records(args) + + report_filepath = args.report + with open(report_filepath, 'w') as report_file: + + report_file.write('Information to extract:\n') + if args.custom_extraction_type == 'True': + for info in args.to_extract[1:-1].split(','): + report_file.write('\t' + info + '\n') + else: + report_file.write('\tsequences\n') + + if constraints != None: + report_file.write('Constraints on extraction:\n') + for constrained_info in formatted_constraints: + report_file.write('\tInfo to constraint: ' + constrained_info + + '\n') + for constraint in formatted_constraints[constrained_info]: + report_file.write('\t\tType of constraint: ' + + constraint.get_raw_constraint_type() + + '\n') + report_file.write('\t\tValues:\n') + values = constraint.get_values() + for value in values: + report_file.write('\t\t\t' + str(value) + '\n') + report_file.write('Number of similarity records: ' + + str(records.get_record_nb()) + '\n') + report_file.write('Number of extracted similarity records: ' + + str(records.get_conserved_record_nb()) + '\n') + +######## +# Main # +######## +if __name__ == "__main__": + parser = argparse.ArgumentParser() + parser.add_argument('--input', required=True) + parser.add_argument('--format', required=True) + parser.add_argument('--custom_extraction_type', required=True) + parser.add_argument('--to_extract') + parser.add_argument('--output_information') + parser.add_argument('--split') + parser.add_argument('--quality_format') + parser.add_argument('--output_sequence') + parser.add_argument('--output_quality') + parser.add_argument('--constraint', action='append') + parser.add_argument('--report', required=True) + args = parser.parse_args() + + convert_extract_sequence_file(args)
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/convert_extract_sequence_file.xml Tue Apr 26 08:17:42 2016 -0400 @@ -0,0 +1,249 @@ +<tool id="convert_extract_sequence_file" name="Convert/ Extract information" version="1.0.0"> + + <description>from a sequence file, with possible constraints</description> + + <macros> + <xml name="extraction_option"> + <param name="to_extract" type="select" display="checkboxes" multiple="true" label="Information to extract" help=""> + <option value="id">Identifiant</option> + <option value="length">Length</option> + <validator type="no_options" message="Select at least one information to extract"/> + </param> + </xml> + + <xml name="extraction_test"> + <param name='specific_extraction' type="select" label="Extract specific information?" help="If no is selected, a sequence file is generated. If yes, a text file containing the wanted information is generated"> + <option value="True">Yes</option> + <option value="False" selected="true">No</option> + </param> + </xml> + </macros> + + <requirements> + </requirements> + + <stdio> + </stdio> + + <version_command> + </version_command> + + <!--<command>--> + <command><![CDATA[ + python $__tool_directory__/convert_extract_sequence_file.py + --input $sequence_file_format.sequence_file + + --custom_extraction_type $sequence_file_format.extraction.specific_extraction + + #if $sequence_file_format.extraction.specific_extraction == "True": + --to_extract "{$sequence_file_format.extraction.to_extract}" + --output_information $information_file + #else if $sequence_file_format.format=="fastq": + --split $sequence_file_format.extraction.split.split_test + #if $sequence_file_format.extraction.split.split_test : + --quality_format $sequence_file_format.extraction.split.quality_format + --output_sequence $fasta_sequence_file_from_fastq + --output_quality $quality_file + #else: + --output_sequence $fastq_sequence_file + #end if + #else: + --output_sequence $fasta_sequence_file + #end if + + #if $constraints.constrained_extraction == "True" : + #for $i, $constrain in enumerate( $constraints.constraint_definition ) + #set info_to_constrain=$constrain.constrained_information['info_to_constrain'] + #if $info_to_constrain in ("id"): + --constraint "$info_to_constrain: + ${constrain.constrained_information.constraint_type.type}: + ${constrain.constrained_information.constraint_type.value}" + #else: + #for $j, $sub_constrain in enumerate( $constrain.constrained_information.constraint_definition ) + --constraint "$info_to_constrain: + ${sub_constrain.type}: + ${sub_constrain.value}" + #end for + #end if + #end for + #end if + + --report $report_filepath + --format $sequence_file_format.format + ]]> + </command> + + <inputs> + <conditional name="sequence_file_format"> + <param name="format" type="select" display="radio" + label="Format of the sequence file" help=""> + <option value="fasta">Fasta</option> + <option value="fastq">FastQ</option> + </param> + <when value="fastq"> + <param name="sequence_file" type="data" format="fastq" + label="Sequence file" help=""/> + <conditional name="extraction"> + <expand macro="extraction_test"/> + + <when value="True"> + <expand macro="extraction_option"/> + </when> + <when value="False"> + <conditional name="split"> + <param name='split_test' type="select" label="Split file into sequence and quality files?" help="If yes is selected, a fasta and a quality file are generated. If no, a fastq file is generated"> + <option value="True" selected="true">Yes</option> + <option value="False">No</option> + </param> + + <when value="True"> + <param name="quality_format" type="select" display="radio" label="Coding of quality scores?" help=""> + <option value="sanger" selected="true">Sanger (Phred+33)</option> + <option value="solexa">Solexa (Solexa+64) </option> + <option value="illumina_1_3">Illumina 1.3+ (Phred+64) </option> + <option value="illumina_1_5">Illumina 1.5+ (Phred+64) </option> + <option value="illumina_1_8">Illumina 1.8+ (Phred+33) </option> + </param> + </when> + <when value="False" /> + </conditional> + </when> + </conditional> + </when> + + <when value="fasta"> + <param name="sequence_file" type="data" format="fasta" + label="Sequence file" help=""/> + <conditional name="extraction"> + <expand macro="extraction_test"/> + + <when value="True"> + <expand macro="extraction_option"/> + </when> + <when value="False" /> + </conditional> + </when> + </conditional> + + <conditional name="constraints"> + <param name='constrained_extraction' type='select' label="Constrain extraction?" help=""> + <option value="True">Yes</option> + <option value="False" selected="true">No</option> + </param> + + <when value="True"> + <repeat name="constraint_definition" title="Constraints on sequences" min="1"> + <conditional name="constrained_information"> + <param name="info_to_constrain" type="select" label="Information to constrain" help=""> + <option value="id">Identifiant</option> + <option value="length">Length</option> + </param> + <when value="id"> + <conditional name="constraint_type"> + <param name="type" type="select" display="radio" label="Type of constraint" help=""> + <option value="equal">Equal a value</option> + <option value="in">In a list</option> + <option value="not_in">Not in a list</option> + </param> + <when value="equal"> + <param name="value" type="text" size="200" label="Equal to" help=""/> + <validator type="empty_field" message="Give a value"/> + </when> + <when value="in"> + <param format="txt" name="value" type="data" label="List of constraint" help="Text file with a value per line and nothing else"/> + <validator type="unspecified_build" message="Select a file"/> + </when> + <when value="not_in"> + <param format="txt" name="value" type="data" label="List of constraint" help="Text file with a value per line and nothing else"/> + <validator type="unspecified_build" message="Select a file"/> + </when> + </conditional> + </when> + <when value="length"> + <repeat name="constraint_definition" title="Constraint on sequence length" min="1"> + <param name="type" type="select" label="Type of constraint" help=""> + <option value="equal">Equal to </option> + <option value="lower">Lower than </option> + <option value="strictly_lower">Strictly lower than </option> + <option value="greater">Greater than </option> + <option value="strictly_greater">Strictly greater than </option> + </param> + <param name="value" type="integer" min="0" max="3000" value="100" label="Value" help=""/> + </repeat> + </when> + </conditional> + </repeat> + </when> + <when value="False" /> + </conditional> /> + </inputs> + + <outputs> + <data format="txt" name="information_file" + label="${tool.name} on ${on_string}: Information"> + <filter>((sequence_file_format['extraction']['specific_extraction'] == "True" ))</filter> + </data> + + <data format="fasta" name="fasta_sequence_file" + label="${tool.name} on ${on_string}: Extracted sequences" > + <filter>((sequence_file_format['format'] == 'fasta' and not sequence_file_format['extraction']['specific_extraction']== "True" ))</filter> + </data> + + <data format="fastq" name="fastq_sequence_file" + label="${tool.name} on ${on_string}: Extracted sequences"> + <filter>((sequence_file_format['format'] == 'fastq' and sequence_file_format['extraction']['specific_extraction'] == "False" and sequence_file_format['extraction']['split']['split_test'] == "False" ))</filter> + </data> + + <data format="qual" name="quality_file" + label="${tool.name} on ${on_string}: Extracted quality"> + <filter>((sequence_file_format['format'] == 'fastq' and sequence_file_format['extraction']['specific_extraction'] == "False" and sequence_file_format['extraction']['split']['split_test'] == "True" ))</filter> + </data> + + <data format="fasta" name="fasta_sequence_file_from_fastq" + label="${tool.name} on ${on_string}: Extracted sequences"> + <filter>((sequence_file_format['format'] == 'fastq' and sequence_file_format['extraction']['specific_extraction'] == "False" and sequence_file_format['extraction']['split']['split_test'] == "True" ))</filter> + </data> + + <data format="txt" name="report_filepath" + label="${tool.name} on ${on_string}: Report"/> + </outputs> + + <tests> + <test> + <param name="format" value="fasta"/> + <param name="sequence_file" value="input_sequence_file.fasta"/> + <param name="specific_extraction" value="True" /> + <param name="to_extract" value="length" /> + <param name="constrained_extraction" value="False" /> + <output name="information_file" file="information_lenght_fasta_output.txt"/> + <output name="report_filepath" file="report_length_fasta_output.txt"/> + </test> + <test> + <param name="format" value="fastq"/> + <param name="sequence_file" value="input_sequence_file.fastq"/> + <param name="specific_extraction" value="False" /> + <param name="split_test" value="True" /> + <param name="quality_format" value="illumina_1_3" /> + <param name="constrained_extraction" value="False" /> + <output name="quality_file" file="extracted_quality_illumina_1_3_fastq_output.qual"/> + <output name="fasta_sequence_file_from_fastq" file="extracted_sequences_illumina_1_3_fastq_output.fasta"/> + <output name="report_filepath" file="report_illumina_1_3_fastq_output.txt"/> + </test> + </tests> + + <help><![CDATA[ + +**What it does** + +This tool extracts information (sequences, id, length, ...) from sequence files or convert a FastQ file to Fasta file. + +Some constraints could be added to extraction/conversion. For example, only sequences with more than 30 bp could be extracted. Or, a sequences whose the identifiant is in a list. + +The input is a sequence file in fasta or fastq format. The tool generates different outputs given the chosen parameters. +]]> + </help> + + <citations> + </citations> +</tool> +
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36 37 36 37 36 36 34 36 27 31 36 31 34 37 +37 31 36 33 32 37 37 36 36 36 37 37 +>HWI-M00234:263:000000000-ADM55:1:1101:27958:6935 1:N:0:ATCACG +34 34 34 34 34 38 38 38 38 38 38 38 38 38 38 38 35 38 38 34 37 38 38 38 38 38 38 38 37 36 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 23 35 34 +27 37 38 38 38 37 37 38 38 38 38 38 38 38 38 38 38 34 38 37 25 37 35 38 38 38 38 38 38 31 37 36 36 36 38 38 38 36 38 35 38 38 38 37 38 38 38 38 38 38 25 37 37 38 38 28 36 38 37 37 +37 34 36 37 38 24 36 36 37 38 35 10 31 31 36 36 35 36 35 23 35 32 29 36 36 34 31 36 37 38 37 32 37 37 38 37 28 37 30 10 19 19 26 34 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 +2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 +2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 +>HWI-M00234:263:000000000-ADM55:1:1101:6151:7404 1:N:0:ATCACG +34 34 34 34 34 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 37 38 37 38 38 38 38 38 +38 38 38 38 38 37 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 38 35 37 23 37 38 38 38 37 38 38 38 38 36 38 38 38 22 10 34 38 38 37 38 38 38 38 38 38 38 38 34 25 23 37 38 38 +38 35 37 38 37 36 38 38 38 37 38 38 38 37 38 34 38 38 38 38 38 38 38 38 35 38 30 36 38 38 38 38 38 37 37 11 27 37 38 38 38 38 37 35 38 38 38 38 11 20 11 36 37 38 38 38 35 10 10 9 +20 25 35 38 37 34 35 35 30 21 29 32 37 37 37 19 30 21 8 20 30 36 33 30 35 15 29 35 37 37 37 32 37 15 7 14 13 21 27 35 15 18 26 18 7 8 13 21 19 21 27 32 8 8 13 8 8 7 19 25 +15 27 33 37 33 35 30 37 27 17 27 32 37 37 27 33 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/extracted_sequences_illumina_1_3_fastq_output.fasta Tue Apr 26 08:17:42 2016 -0400 @@ -0,0 +1,174 @@ +>HWI-M00234:263:000000000-ADM55:1:1101:21704:2233 1:N:0:ATCACG +GTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTC +CAACTTCATACAGGCGGGTTTCAGCC +>HWI-M00234:263:000000000-ADM55:1:1101:21334:2699 1:N:0:ATCACG +CTTTTGACGGCCTTTTTGTTGTCTTCTGAATTTTGTTTTTTTCGGTTGTAACATAATTCC +TAAAAATCAAATTCGTTAGCGATTATTTTTCTTTCTTTTGAAGTTCTTTCCGCCATTGTT +TCCGCTATTGCTTCCACGACC +>HWI-M00234:263:000000000-ADM55:1:1101:13968:2885 1:N:0:ATCACG +CCCACTGCTGCCTCCCGTAGGAGCCTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACC +CTCTCAGGTCGGCTACCCATCGTTGCCCTGGTAAGCCATTACCCTACCAACTAGCTAATG +GGACGCGGGCACATCTCAAAGCGGATTACCCCTTTGGTTAAAGTTTAATGCGAAACTCTA +ACTTTATGCGTTATTAACCCTCCTTTCGGAGGGCTATTCCCCTCTTTGATGCAGGTTGCC +CACGTGTTACTCACCCGTCCGCCGCTAATCCACTTCCCGAAGGCAGATTCAACCCTCGAT +>HWI-M00234:263:000000000-ADM55:1:1101:11873:2948 1:N:0:ATCACG +CCCCGTTACATCTTCCGCGCAGGAAGACTCGATCAGTGAGCTATTACGCTTTCTTTGAAG +GATGGCTGCTTCTAAGCCAACTTCCTGACTGTCTTAGCCTTCCCACTTCGTTTTCCACTT +AGTCAATATTAGGGACCTTAGCCGGCGGTCTGGGTTGTTTCCCTCTTGAGTCCGGACGTT +AGCACCCGGTGCTCTGTCCCCCGTGCCCAAACTTCCAAGTATTCGGAGTTTGCCATGGTT +TGGTAAGACGCTATGTCCCCCTAACCATAACAGTGTTCTACCCTCTGAACACATACTCGA +>HWI-M00234:263:000000000-ADM55:1:1101:7855:3066 1:N:0:ATCACG +CGTCAATCTTCCAACGCCCACGGCAGATAGGGACAAAACTGTCTCACGACGTTTTAAACC +CAGCTCACGTACCTCTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTACAGC +>HWI-M00234:263:000000000-ADM55:1:1101:24817:3238 1:N:0:ATCACG +CCCGTGACCAGATTCATGGCCGCCGGTTAGAACCCCAGTACTGTCAGGGTGGTATCCCAA +GGA +>HWI-M00234:263:000000000-ADM55:1:1101:17179:3527 1:N:0:ATCACG +GTGCTTATTTCACTTCCTCAAAATCAGCATCCTGCACATTATCGCCATGCTTT +>HWI-M00234:263:000000000-ADM55:1:1101:21254:3571 1:N:0:ATCACG +GTCGCGGGGAAGATCAACTCCTGCAATTCGAGCCATACCAATTACCTCCTAGCGCCCTGA +CGCTGTTTATGACGAGGATCGCGGCTGCAGATGACGCGAATGACGCCATTGCGCTTGATC +ACTCGGCAATACTCACAAATGGGTTTAACAGATTATCTAACCTTCATAGTTTGCACACTC +CTCATGGACATTCC +>HWI-M00234:263:000000000-ADM55:1:1101:16569:3709 1:N:0:ATCACG +CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTGGAATACTTATTG +CGTTTGCTGCGGCACCGAATGGCTTTGCCACCCGACACCTAGTATTCATCGTTTACGGCG +TGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGGA +TCGGAAGAGCACACGTCTGAACTCCAGTCACATCCCG +>HWI-M00234:263:000000000-ADM55:1:1101:22403:3828 1:N:0:ATCACG +CCCGGACATCTTCGGCGCAGGATCTCTTGACTAGTGAGCTATTACGCACTCTTTAAATGA +GTGGCTGCTTCTAAGCCAACATCCTAGTTGTCTTAGAAATCCCACATCCTTTTCCACTTA +ACTTACACTTTGGGACCTTAGCTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA +CATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATATCAATCAAACACAC +ACACACCAGCTTAC +>HWI-M00234:263:000000000-ADM55:1:1101:7508:4067 1:N:0:ATCACG +GGTGCACTAGGATCGTAGTTGGCTACTTTCCCGTTTTCAATGTATACGCAAGGTACACGG +TCAGCGGTAGCTGCCATAATGTAATGGTAGTCAAATCCTAAGTCTCCCAATGCACATGGC +AGTGGAGCGTTCCAATCTTGTGTACCGGCTTTGTCTCCTAATCCCAAGTGCCATTTTCCG +ATAGCTCCGGTAGCATAACCGGTACTTTTGAACATAACCGCCATGGTATATTGTTCGGGA +CGGATAATCATTCCGGCATTTCCGGCAGCTACG +>HWI-M00234:263:000000000-ADM55:1:1101:26232:4161 1:N:0:ATCACG +CTCTTCTTAAGCTCGAACAGCTTCTTCAGTCTTACCTGCCATTTCATCTAAAATTTTTAA +AGCTCGCTCTAAAACAGTAGTATCATCAATCATAACCAATCCACCATCTGGTCCAGGTTC +CAAGTGAATCCCAACACTTTTCCCCTCCTTAAAATTGTGTCCACCGAAAAAATTTCTTAC +AGTGTCAACATTCAGTCCAAGTTCATCGGCTATTCTATGCATACTACCACTAGGCAATGA +GT +>HWI-M00234:263:000000000-ADM55:1:1101:23151:4189 1:N:0:ATCACG +GCGGATTTTCTCGGGAGTATGATTACCCACACTATTGGATTCTTCCGAAGAAGACTCCAT +ACTACCAAGTTCAGCTCGGATGGTGGATTTGCCTGCCAAGATCAGAAGAGCACACGTCTG +AACTCCAGTCACATCACGACTTCGTATGCCGTATTCTGCTTGAAAAAAACAATGAAAACT +AGTCACCGATGACTCACGTTCGCTGAACATTATCTGACAACTAGCTTAACAATGGACATG +CTTAGCACTGACAACCAGACG +>HWI-M00234:263:000000000-ADM55:1:1101:17148:4803 1:N:0:ATCACG +CCCTTTTCGGCAATGGCGATGTTACGGTACATAAGAGCGATTTCGGGGAAACCTTCTTCG +TCTGCAATATCAGCAAACTTAGGATAATCCAATGA +>HWI-M00234:263:000000000-ADM55:1:1101:8939:4808 1:N:0:ATCACG +CCACAGTTCTGTCATATCGCAGTACAGGAATCTCAACCTGTTGTCCATCGGCTACGGCTC +TCGCCCTCGCCTTAGGCCCCGACTTACCCAGGGCAGATCAGCTTTA +>HWI-M00234:263:000000000-ADM55:1:1101:11727:5103 1:N:0:ATCACG +CTCCCGTATAGGATGGATCGAAAGCAATCGTCAGGAATCCACGTTCTGCCAATGTCTGCG +CATACAAGCCCGAAGCTTGTTCCTTCACGGCACCAAATGGGCCACTGATGGCAATGGCAG +GCAATTTACCCGTCACATTCTTGGGTACATACAAATCGGCGGCAAGTGTAATGCCGTAAC +GGTTGTGGAAAG +>HWI-M00234:263:000000000-ADM55:1:1101:15029:5173 1:N:0:ATCACG +GGCCTATTCTCTGCGGCCGCCTTTCGGCGGCACCCCTTCTCCCGAAGTTACGGGGTCATT +TTGCCGAGTTCCTTAACAATGCTTCTTCCGTCGGCCTTAGGATTCTCTCCTCATCCACCT +GTGTCGGTTTACGGTACGGGTACAATATAAACAATAGCGGCTTTTCTTGACGGCCGGCTC +ATGGGCTTCCCTACTTTAGT +>HWI-M00234:263:000000000-ADM55:1:1101:7330:5248 1:N:0:ATCACG +TGTGTATGAATGAGCGGCTACAGGCTTAGTCGATTTCTTTACTGCGCAGAAGCGGGACTG +TGACCGACAAAACAGCCGTCGCCTCTCTACGTTCCGGGACCATACGCCGCTCGCAGTGAT +TGACCCTTACAACCTTGCGGGCGCTCTC +>HWI-M00234:263:000000000-ADM55:1:1101:18630:5353 1:N:0:ATCACG +GGCGTGTTCTGCCAAAATAGTGCTGGGCAATATACGACATAGAAGCGAAAGGCAATACTT +TTTCTAAGGCCTGCCTGATTTCTACTGTTTTCACAATGGCTTGGGCTTCATTGATTGATT +GCCTGGCACCATCTTGGAATGCTTGCGCAAACGCTTTTTTATCCTCCGGTGAAAGCGTCT +GCAGAAAAGCCCTGAAACGTTTTTTATGGTCGGCCACTTCCTCAGGAGTATTG +>HWI-M00234:263:000000000-ADM55:1:1101:11799:5476 1:N:0:ATCACG +CCTAAATTTTCGAGTATAAGCAGGACCGAAGCCACCAACTTATACCTTATCCTCTAAATT +TTCGTAAGAGACATGAAGCGGATCTCTAACTATTCCCGATTTTACTGCACCACTTGATCA +AACGCTTCGGAACAAGAGCTTTTGAGTGATGTCTCGTTTCCACCACTTTGGCAGAAATAA +AGCTAATCTACTATGATTCGATTAAGCAGCAAGAGCATAATTTTCGTTGCCAGATAAAAT +TT +>HWI-M00234:263:000000000-ADM55:1:1101:11348:5668 1:N:0:ATCACG +GCCTAAATTTTCGTGCAGCGGCAGAACCAAAGCCATCAACCGTTGCCTTATCCTCTAAAT +TTTCACCCGCGCCACGAGGCTGACACGAACTATCCCCGATTTGACTGCACCACTTTATCG +GAATGCTTCGGAGCAACAGCTTCCGAGTGATGTCACGTCCCAGCACCTGGTGCCGGGATT +AAGCTTCAATCTACTATACTTCGATTAAGCAGCGAGAGCGTAACGAGTTTCGCCAGATAA +AATTTTGAGGACTGAGATTAAAGTGAAAATC +>HWI-M00234:263:000000000-ADM55:1:1101:25874:5826 1:N:0:ATCACG +CCTGCAGTGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGTACCTCT +TTAATGGGCGAACAGCCCAACCCTTGGGACCTTCTCCAGCCCCAGGATGAGATGAGCCGA +CATCGAGGTGCCAAACTTTCCCGCCGATATGGACGCCCGGGCAAAATCAGCTTGTTATCC +CCAGGGTAGCTTTTATCCGTTGAGCGACGACCCTTCCATTCGGAATCGCCGGAATACTAT +GCCCTGCTTTCGCACCTGCCCAACCAGTCCGCCCAGCAGTTAAG +>HWI-M00234:263:000000000-ADM55:1:1101:26932:5861 1:N:0:ATCACG +AGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGTGTTCTTCCTAATCTCTACGC +ATTTCACCGCTACACTAGGAATTCCGCTTTCCTCTCCTGCACTCTAGATATCCAGTTTGG +AATGCAGCCCCCAGGTTAAGCCCGGGGATTTCACATCCCACTTAAACATCCGCCTACGCA +CCCTTTACCACCAGTAAATCCGGACAACGCTCGCCACCTACGTATTACCGCGGCTGCTGG +CACGTAGTTAGCCGTGGCTTCCTCCTCTGGTACCTTCATTATCGTCCCCGAAACC +>HWI-M00234:263:000000000-ADM55:1:1101:14154:5990 1:N:0:ATCACG +GTGGGATGTCAAGGTAAGCGGCGGCAGCCTTGGCTACAGCAAGAGATACGCCGAGGATGG +CATTGGCACCCAGATTGGCTTTCGTTTTGGTTCCGTCCAGTGCAAGCATGGCATGGTCGA +TACCCATCTGGTCGAGGGCGGACATACCGATGAGCTTGGGAGCAATGATATTATTGATAT +TGTCCACGGCTTTCAGGAC +>HWI-M00234:263:000000000-ADM55:1:1101:25022:6229 1:N:0:ATCACG +CCCGAGAACGTATTCACCGCAACATTGCTGATTTGCGATTACTAGTGATTCCAACTTCAT +GTACTCGAGTTGCAGAGTACAATCCGAACTACGAACAGCTTTCTGAGGTTTGCTCCTCCT +CGCAGATTTGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC +>HWI-M00234:263:000000000-ADM55:1:1101:21023:6286 1:N:0:ATCACG +GTGTACAAGGCCCGGGAACGTATTCACCGCGCCGTGGCTGATGCGCGATTACTAGCGAAT +CCAGCTTCATGGAGTCGGGTTGCAGACTCCAATCCGAACTGAGAGAGGCTTTAGGGATTA +GCATCACGTCGCCGTGTAGCTGCCTTCTGTACCCCCCATTGTAACACGTGTGTAGCCCCG +GACGTAAGGGCCGTGCTGATTTGCCGTCATCCCCACCTTCCTCAC +>HWI-M00234:263:000000000-ADM55:1:1101:8138:6336 1:N:0:ATCACG +TGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCAACT +CCAGCTTCATGTAGGCGAGTTGCAGCCTACAATCCGAACTGAGATAGGTTTTATAAGTTT +TGCTCCACCTCACGGTCTT +>HWI-M00234:263:000000000-ADM55:1:1101:11055:6543 1:N:0:ATCACG +CCTGGACATTTTCGGCGCAGGACCTCCCGACCAGTGAGCTATTACGCACTCTTTGACTGT +GTGGCTGCTTCTGAGCCAACATCCTGGTTGACTTTGAAATCTCACATACTTTTCCACTTA +ACACGCACTTTGGGACCTTAGCTGGAGGTCTTGGCTCTTTCCTTTTTGCCTATCCAACTT +ATCTCGTATAGTCTGACTCCCGG +>HWI-M00234:263:000000000-ADM55:1:1101:24980:6575 1:N:0:ATCACG +GCCATTTATCTACGCCTGTTGTCACCAACAGGCTCTAGCGGTCTACCCTCCGACGTGGGG +CGAGCAACCCTCATA +>HWI-M00234:263:000000000-ADM55:1:1101:7596:6682 1:N:0:ATCACG +GCCCCCGTCAATTCCTTTGAGTTTCACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTA +ACGCTTTCGCTTGGCGGCTGACAATGTATCGCCAACCGCGAGTATTCATCAGATCGGAAG +AGCACACGTCTGAACTCCAGTCACATCCCGATCTCGTATGCCGTCCTCGGCTTGAAAAAA +AAAAATATAAACCATGATGAATTTCGAACACTCACGACCACACACTAAGCGTCGACGTA +>HWI-M00234:263:000000000-ADM55:1:1101:24419:6736 1:N:0:ATCACG +CGGGCGTTGGCAAGTACGTTATATCACTTACCCACGCTCGATTCAGAACCCCAGGAGTAA +ATGCTCGTTTTAAGAGGTTGGGATACACAGGCATGGTGTGATTACTGTCGGTGGTTTTTA +CGGTTCGCTTAAAAAAGCGGTAACCAATGATGCCATTAAAGCGAAGGATAGCGCGTAAAC +GTTTGACACCAACTTTCACTCCACGGTGTTGCATCAAAGC +>HWI-M00234:263:000000000-ADM55:1:1101:5068:6755 1:N:0:ATCACG +GCAGCGGCAGAACCAAAGCCATCAACCGTTGCCTTATCCTCTAAATTTTCACCCGCGCCA +CGAGGCTGACACGAACTATCCCCGATTTGACTGCACCACTTTATCGGAATGCTTCGGAGC +AACAGCTTCCGAGTGATGTCACGTCCCAGCACCTGGTGCCGGGATTAAGCTTCAATCTAC +TATACTTCGATTAAGCAGCGAGAGCGTAACGAGTTTCGCCAGATAAAATTTTGAGGACTG +AGATTAAAGTGCAAATC +>HWI-M00234:263:000000000-ADM55:1:1101:21477:6796 1:N:0:ATCACG +CCCCACTGCTGCTTCCCGTAGGAGTTTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTT +CCTCTCAGAACCCCTATCCATCGTTGACTAGGTGGGCCGTTACCCCGCCTACTATCTAAT +GGAACGCATCCCCATCGTCTACCGGAAAATACCTT +>HWI-M00234:263:000000000-ADM55:1:1101:12483:6880 1:N:0:ATCACG +CTCCTTGTCTGCCTTCGATTACGGCATTTGCAATCGTTCCTGTAAGTAGTTTTACAGCTC +TAATGGCATCGTCATTCCCTGGTATTACGTAGTCTATTTCATCAGGATCACAGTTAGTAT +CAACTATTGCTACAACTGGTATTCCTAATATCTTCGCTTCATTTACTGCAATTCTTTCTT +TTCTTGGATCTACAACAAATAATACATCAGGAAGACCACCCATATCTTGAATGCCACCTA +AAAACTTTTCAA +>HWI-M00234:263:000000000-ADM55:1:1101:27958:6935 1:N:0:ATCACG +CCATTCGGAAATCCGCGGATCAAAGGTCATTTGCACCTACCCGCAGCTTATCGCAGCTTA +TCACGTCCTTCATCGCCTCCGAGAGCCAAGAGATCGGAAGAGCACACGTCTGAACTCCAG +TCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCCACAAATTCACACT +ACACACCCACCTCCCATCACGCATCTCTTTTGTCCGAGTCACGCTGCACGCTACCTGCAC +ACTACCTGCCTCAGTTACATCTTTTTACTCAATGTCCCACCATTTATCAC +>HWI-M00234:263:000000000-ADM55:1:1101:6151:7404 1:N:0:ATCACG +GCACGTAGTTAGCCGGGGCTTCCTCCTAAGGTACCGTCATTATCGTCCCTTAGGACAGAG +CTTTACGATCCGAAAACCTTCATCGCTCACGCGGCGTCGCTGCATCAGAGTTCCCTCCAT +TGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG +TGGCCGTTCACCCTCCCAGGCCGGCTACCCATCGTCGCCTTGGTTAGCTATTACCTCCCC +AACTAGCTAATGGGACGCGAGTCCAGCTTATACCACTTAACCGCTTTTGCTTGAAAATCA
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/information_lenght_fasta_output.txt Tue Apr 26 08:17:42 2016 -0400 @@ -0,0 +1,37 @@ +length +86 +141 +300 +300 +116 +63 +53 +194 +217 +254 +273 +242 +261 +95 +106 +192 +200 +148 +233 +242 +271 +284 +295 +199 +168 +225 +139 +203 +75 +239 +220 +257 +155 +252 +290 +300
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/input_sequence_file.fasta Tue Apr 26 08:17:42 2016 -0400 @@ -0,0 +1,174 @@ +>HWI-M00234:263:000000000-ADM55:1:1101:21704:2233 1:N:0:ATCACG +GTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTC +CAACTTCATACAGGCGGGTTTCAGCC +>HWI-M00234:263:000000000-ADM55:1:1101:21334:2699 1:N:0:ATCACG +CTTTTGACGGCCTTTTTGTTGTCTTCTGAATTTTGTTTTTTTCGGTTGTAACATAATTCC +TAAAAATCAAATTCGTTAGCGATTATTTTTCTTTCTTTTGAAGTTCTTTCCGCCATTGTT +TCCGCTATTGCTTCCACGACC +>HWI-M00234:263:000000000-ADM55:1:1101:13968:2885 1:N:0:ATCACG +CCCACTGCTGCCTCCCGTAGGAGCCTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACC +CTCTCAGGTCGGCTACCCATCGTTGCCCTGGTAAGCCATTACCCTACCAACTAGCTAATG +GGACGCGGGCACATCTCAAAGCGGATTACCCCTTTGGTTAAAGTTTAATGCGAAACTCTA +ACTTTATGCGTTATTAACCCTCCTTTCGGAGGGCTATTCCCCTCTTTGATGCAGGTTGCC +CACGTGTTACTCACCCGTCCGCCGCTAATCCACTTCCCGAAGGCAGATTCAACCCTCGAT +>HWI-M00234:263:000000000-ADM55:1:1101:11873:2948 1:N:0:ATCACG +CCCCGTTACATCTTCCGCGCAGGAAGACTCGATCAGTGAGCTATTACGCTTTCTTTGAAG +GATGGCTGCTTCTAAGCCAACTTCCTGACTGTCTTAGCCTTCCCACTTCGTTTTCCACTT +AGTCAATATTAGGGACCTTAGCCGGCGGTCTGGGTTGTTTCCCTCTTGAGTCCGGACGTT +AGCACCCGGTGCTCTGTCCCCCGTGCCCAAACTTCCAAGTATTCGGAGTTTGCCATGGTT +TGGTAAGACGCTATGTCCCCCTAACCATAACAGTGTTCTACCCTCTGAACACATACTCGA +>HWI-M00234:263:000000000-ADM55:1:1101:7855:3066 1:N:0:ATCACG +CGTCAATCTTCCAACGCCCACGGCAGATAGGGACAAAACTGTCTCACGACGTTTTAAACC +CAGCTCACGTACCTCTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTACAGC +>HWI-M00234:263:000000000-ADM55:1:1101:24817:3238 1:N:0:ATCACG +CCCGTGACCAGATTCATGGCCGCCGGTTAGAACCCCAGTACTGTCAGGGTGGTATCCCAA +GGA +>HWI-M00234:263:000000000-ADM55:1:1101:17179:3527 1:N:0:ATCACG +GTGCTTATTTCACTTCCTCAAAATCAGCATCCTGCACATTATCGCCATGCTTT +>HWI-M00234:263:000000000-ADM55:1:1101:21254:3571 1:N:0:ATCACG +GTCGCGGGGAAGATCAACTCCTGCAATTCGAGCCATACCAATTACCTCCTAGCGCCCTGA +CGCTGTTTATGACGAGGATCGCGGCTGCAGATGACGCGAATGACGCCATTGCGCTTGATC +ACTCGGCAATACTCACAAATGGGTTTAACAGATTATCTAACCTTCATAGTTTGCACACTC +CTCATGGACATTCC +>HWI-M00234:263:000000000-ADM55:1:1101:16569:3709 1:N:0:ATCACG +CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTGGAATACTTATTG +CGTTTGCTGCGGCACCGAATGGCTTTGCCACCCGACACCTAGTATTCATCGTTTACGGCG +TGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGGA +TCGGAAGAGCACACGTCTGAACTCCAGTCACATCCCG +>HWI-M00234:263:000000000-ADM55:1:1101:22403:3828 1:N:0:ATCACG +CCCGGACATCTTCGGCGCAGGATCTCTTGACTAGTGAGCTATTACGCACTCTTTAAATGA +GTGGCTGCTTCTAAGCCAACATCCTAGTTGTCTTAGAAATCCCACATCCTTTTCCACTTA +ACTTACACTTTGGGACCTTAGCTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA +CATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATATCAATCAAACACAC +ACACACCAGCTTAC +>HWI-M00234:263:000000000-ADM55:1:1101:7508:4067 1:N:0:ATCACG +GGTGCACTAGGATCGTAGTTGGCTACTTTCCCGTTTTCAATGTATACGCAAGGTACACGG +TCAGCGGTAGCTGCCATAATGTAATGGTAGTCAAATCCTAAGTCTCCCAATGCACATGGC +AGTGGAGCGTTCCAATCTTGTGTACCGGCTTTGTCTCCTAATCCCAAGTGCCATTTTCCG +ATAGCTCCGGTAGCATAACCGGTACTTTTGAACATAACCGCCATGGTATATTGTTCGGGA +CGGATAATCATTCCGGCATTTCCGGCAGCTACG +>HWI-M00234:263:000000000-ADM55:1:1101:26232:4161 1:N:0:ATCACG +CTCTTCTTAAGCTCGAACAGCTTCTTCAGTCTTACCTGCCATTTCATCTAAAATTTTTAA +AGCTCGCTCTAAAACAGTAGTATCATCAATCATAACCAATCCACCATCTGGTCCAGGTTC +CAAGTGAATCCCAACACTTTTCCCCTCCTTAAAATTGTGTCCACCGAAAAAATTTCTTAC +AGTGTCAACATTCAGTCCAAGTTCATCGGCTATTCTATGCATACTACCACTAGGCAATGA +GT +>HWI-M00234:263:000000000-ADM55:1:1101:23151:4189 1:N:0:ATCACG +GCGGATTTTCTCGGGAGTATGATTACCCACACTATTGGATTCTTCCGAAGAAGACTCCAT +ACTACCAAGTTCAGCTCGGATGGTGGATTTGCCTGCCAAGATCAGAAGAGCACACGTCTG +AACTCCAGTCACATCACGACTTCGTATGCCGTATTCTGCTTGAAAAAAACAATGAAAACT +AGTCACCGATGACTCACGTTCGCTGAACATTATCTGACAACTAGCTTAACAATGGACATG +CTTAGCACTGACAACCAGACG +>HWI-M00234:263:000000000-ADM55:1:1101:17148:4803 1:N:0:ATCACG +CCCTTTTCGGCAATGGCGATGTTACGGTACATAAGAGCGATTTCGGGGAAACCTTCTTCG +TCTGCAATATCAGCAAACTTAGGATAATCCAATGA +>HWI-M00234:263:000000000-ADM55:1:1101:8939:4808 1:N:0:ATCACG +CCACAGTTCTGTCATATCGCAGTACAGGAATCTCAACCTGTTGTCCATCGGCTACGGCTC +TCGCCCTCGCCTTAGGCCCCGACTTACCCAGGGCAGATCAGCTTTA +>HWI-M00234:263:000000000-ADM55:1:1101:11727:5103 1:N:0:ATCACG +CTCCCGTATAGGATGGATCGAAAGCAATCGTCAGGAATCCACGTTCTGCCAATGTCTGCG +CATACAAGCCCGAAGCTTGTTCCTTCACGGCACCAAATGGGCCACTGATGGCAATGGCAG +GCAATTTACCCGTCACATTCTTGGGTACATACAAATCGGCGGCAAGTGTAATGCCGTAAC +GGTTGTGGAAAG +>HWI-M00234:263:000000000-ADM55:1:1101:15029:5173 1:N:0:ATCACG +GGCCTATTCTCTGCGGCCGCCTTTCGGCGGCACCCCTTCTCCCGAAGTTACGGGGTCATT +TTGCCGAGTTCCTTAACAATGCTTCTTCCGTCGGCCTTAGGATTCTCTCCTCATCCACCT +GTGTCGGTTTACGGTACGGGTACAATATAAACAATAGCGGCTTTTCTTGACGGCCGGCTC +ATGGGCTTCCCTACTTTAGT +>HWI-M00234:263:000000000-ADM55:1:1101:7330:5248 1:N:0:ATCACG +TGTGTATGAATGAGCGGCTACAGGCTTAGTCGATTTCTTTACTGCGCAGAAGCGGGACTG +TGACCGACAAAACAGCCGTCGCCTCTCTACGTTCCGGGACCATACGCCGCTCGCAGTGAT +TGACCCTTACAACCTTGCGGGCGCTCTC +>HWI-M00234:263:000000000-ADM55:1:1101:18630:5353 1:N:0:ATCACG +GGCGTGTTCTGCCAAAATAGTGCTGGGCAATATACGACATAGAAGCGAAAGGCAATACTT +TTTCTAAGGCCTGCCTGATTTCTACTGTTTTCACAATGGCTTGGGCTTCATTGATTGATT +GCCTGGCACCATCTTGGAATGCTTGCGCAAACGCTTTTTTATCCTCCGGTGAAAGCGTCT +GCAGAAAAGCCCTGAAACGTTTTTTATGGTCGGCCACTTCCTCAGGAGTATTG +>HWI-M00234:263:000000000-ADM55:1:1101:11799:5476 1:N:0:ATCACG +CCTAAATTTTCGAGTATAAGCAGGACCGAAGCCACCAACTTATACCTTATCCTCTAAATT +TTCGTAAGAGACATGAAGCGGATCTCTAACTATTCCCGATTTTACTGCACCACTTGATCA +AACGCTTCGGAACAAGAGCTTTTGAGTGATGTCTCGTTTCCACCACTTTGGCAGAAATAA +AGCTAATCTACTATGATTCGATTAAGCAGCAAGAGCATAATTTTCGTTGCCAGATAAAAT +TT +>HWI-M00234:263:000000000-ADM55:1:1101:11348:5668 1:N:0:ATCACG +GCCTAAATTTTCGTGCAGCGGCAGAACCAAAGCCATCAACCGTTGCCTTATCCTCTAAAT +TTTCACCCGCGCCACGAGGCTGACACGAACTATCCCCGATTTGACTGCACCACTTTATCG +GAATGCTTCGGAGCAACAGCTTCCGAGTGATGTCACGTCCCAGCACCTGGTGCCGGGATT +AAGCTTCAATCTACTATACTTCGATTAAGCAGCGAGAGCGTAACGAGTTTCGCCAGATAA +AATTTTGAGGACTGAGATTAAAGTGAAAATC +>HWI-M00234:263:000000000-ADM55:1:1101:25874:5826 1:N:0:ATCACG +CCTGCAGTGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGTACCTCT +TTAATGGGCGAACAGCCCAACCCTTGGGACCTTCTCCAGCCCCAGGATGAGATGAGCCGA +CATCGAGGTGCCAAACTTTCCCGCCGATATGGACGCCCGGGCAAAATCAGCTTGTTATCC +CCAGGGTAGCTTTTATCCGTTGAGCGACGACCCTTCCATTCGGAATCGCCGGAATACTAT +GCCCTGCTTTCGCACCTGCCCAACCAGTCCGCCCAGCAGTTAAG +>HWI-M00234:263:000000000-ADM55:1:1101:26932:5861 1:N:0:ATCACG +AGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGTGTTCTTCCTAATCTCTACGC +ATTTCACCGCTACACTAGGAATTCCGCTTTCCTCTCCTGCACTCTAGATATCCAGTTTGG +AATGCAGCCCCCAGGTTAAGCCCGGGGATTTCACATCCCACTTAAACATCCGCCTACGCA +CCCTTTACCACCAGTAAATCCGGACAACGCTCGCCACCTACGTATTACCGCGGCTGCTGG +CACGTAGTTAGCCGTGGCTTCCTCCTCTGGTACCTTCATTATCGTCCCCGAAACC +>HWI-M00234:263:000000000-ADM55:1:1101:14154:5990 1:N:0:ATCACG +GTGGGATGTCAAGGTAAGCGGCGGCAGCCTTGGCTACAGCAAGAGATACGCCGAGGATGG +CATTGGCACCCAGATTGGCTTTCGTTTTGGTTCCGTCCAGTGCAAGCATGGCATGGTCGA +TACCCATCTGGTCGAGGGCGGACATACCGATGAGCTTGGGAGCAATGATATTATTGATAT +TGTCCACGGCTTTCAGGAC +>HWI-M00234:263:000000000-ADM55:1:1101:25022:6229 1:N:0:ATCACG +CCCGAGAACGTATTCACCGCAACATTGCTGATTTGCGATTACTAGTGATTCCAACTTCAT +GTACTCGAGTTGCAGAGTACAATCCGAACTACGAACAGCTTTCTGAGGTTTGCTCCTCCT +CGCAGATTTGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC +>HWI-M00234:263:000000000-ADM55:1:1101:21023:6286 1:N:0:ATCACG +GTGTACAAGGCCCGGGAACGTATTCACCGCGCCGTGGCTGATGCGCGATTACTAGCGAAT +CCAGCTTCATGGAGTCGGGTTGCAGACTCCAATCCGAACTGAGAGAGGCTTTAGGGATTA +GCATCACGTCGCCGTGTAGCTGCCTTCTGTACCCCCCATTGTAACACGTGTGTAGCCCCG +GACGTAAGGGCCGTGCTGATTTGCCGTCATCCCCACCTTCCTCAC +>HWI-M00234:263:000000000-ADM55:1:1101:8138:6336 1:N:0:ATCACG +TGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCAACT +CCAGCTTCATGTAGGCGAGTTGCAGCCTACAATCCGAACTGAGATAGGTTTTATAAGTTT +TGCTCCACCTCACGGTCTT +>HWI-M00234:263:000000000-ADM55:1:1101:11055:6543 1:N:0:ATCACG +CCTGGACATTTTCGGCGCAGGACCTCCCGACCAGTGAGCTATTACGCACTCTTTGACTGT +GTGGCTGCTTCTGAGCCAACATCCTGGTTGACTTTGAAATCTCACATACTTTTCCACTTA +ACACGCACTTTGGGACCTTAGCTGGAGGTCTTGGCTCTTTCCTTTTTGCCTATCCAACTT +ATCTCGTATAGTCTGACTCCCGG +>HWI-M00234:263:000000000-ADM55:1:1101:24980:6575 1:N:0:ATCACG +GCCATTTATCTACGCCTGTTGTCACCAACAGGCTCTAGCGGTCTACCCTCCGACGTGGGG +CGAGCAACCCTCATA +>HWI-M00234:263:000000000-ADM55:1:1101:7596:6682 1:N:0:ATCACG +GCCCCCGTCAATTCCTTTGAGTTTCACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTA +ACGCTTTCGCTTGGCGGCTGACAATGTATCGCCAACCGCGAGTATTCATCAGATCGGAAG +AGCACACGTCTGAACTCCAGTCACATCCCGATCTCGTATGCCGTCCTCGGCTTGAAAAAA +AAAAATATAAACCATGATGAATTTCGAACACTCACGACCACACACTAAGCGTCGACGTA +>HWI-M00234:263:000000000-ADM55:1:1101:24419:6736 1:N:0:ATCACG +CGGGCGTTGGCAAGTACGTTATATCACTTACCCACGCTCGATTCAGAACCCCAGGAGTAA +ATGCTCGTTTTAAGAGGTTGGGATACACAGGCATGGTGTGATTACTGTCGGTGGTTTTTA +CGGTTCGCTTAAAAAAGCGGTAACCAATGATGCCATTAAAGCGAAGGATAGCGCGTAAAC +GTTTGACACCAACTTTCACTCCACGGTGTTGCATCAAAGC +>HWI-M00234:263:000000000-ADM55:1:1101:5068:6755 1:N:0:ATCACG +GCAGCGGCAGAACCAAAGCCATCAACCGTTGCCTTATCCTCTAAATTTTCACCCGCGCCA +CGAGGCTGACACGAACTATCCCCGATTTGACTGCACCACTTTATCGGAATGCTTCGGAGC +AACAGCTTCCGAGTGATGTCACGTCCCAGCACCTGGTGCCGGGATTAAGCTTCAATCTAC +TATACTTCGATTAAGCAGCGAGAGCGTAACGAGTTTCGCCAGATAAAATTTTGAGGACTG +AGATTAAAGTGCAAATC +>HWI-M00234:263:000000000-ADM55:1:1101:21477:6796 1:N:0:ATCACG +CCCCACTGCTGCTTCCCGTAGGAGTTTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTT +CCTCTCAGAACCCCTATCCATCGTTGACTAGGTGGGCCGTTACCCCGCCTACTATCTAAT +GGAACGCATCCCCATCGTCTACCGGAAAATACCTT +>HWI-M00234:263:000000000-ADM55:1:1101:12483:6880 1:N:0:ATCACG +CTCCTTGTCTGCCTTCGATTACGGCATTTGCAATCGTTCCTGTAAGTAGTTTTACAGCTC +TAATGGCATCGTCATTCCCTGGTATTACGTAGTCTATTTCATCAGGATCACAGTTAGTAT +CAACTATTGCTACAACTGGTATTCCTAATATCTTCGCTTCATTTACTGCAATTCTTTCTT +TTCTTGGATCTACAACAAATAATACATCAGGAAGACCACCCATATCTTGAATGCCACCTA +AAAACTTTTCAA +>HWI-M00234:263:000000000-ADM55:1:1101:27958:6935 1:N:0:ATCACG +CCATTCGGAAATCCGCGGATCAAAGGTCATTTGCACCTACCCGCAGCTTATCGCAGCTTA +TCACGTCCTTCATCGCCTCCGAGAGCCAAGAGATCGGAAGAGCACACGTCTGAACTCCAG +TCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCCACAAATTCACACT +ACACACCCACCTCCCATCACGCATCTCTTTTGTCCGAGTCACGCTGCACGCTACCTGCAC +ACTACCTGCCTCAGTTACATCTTTTTACTCAATGTCCCACCATTTATCAC +>HWI-M00234:263:000000000-ADM55:1:1101:6151:7404 1:N:0:ATCACG +GCACGTAGTTAGCCGGGGCTTCCTCCTAAGGTACCGTCATTATCGTCCCTTAGGACAGAG +CTTTACGATCCGAAAACCTTCATCGCTCACGCGGCGTCGCTGCATCAGAGTTCCCTCCAT +TGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTG +TGGCCGTTCACCCTCCCAGGCCGGCTACCCATCGTCGCCTTGGTTAGCTATTACCTCCCC +AACTAGCTAATGGGACGCGAGTCCAGCTTATACCACTTAACCGCTTTTGCTTGAAAATCA
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/input_sequence_file.fastq Tue Apr 26 08:17:42 2016 -0400 @@ -0,0 +1,144 @@ +@HWI-M00234:263:000000000-ADM55:1:1101:21704:2233 1:N:0:ATCACG +GTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAACTTCATACAGGCGGGTTTCAGCC ++ +BCCCCGGED8DFGC::+@C@9@,,EGFAF@CC7@@@FGAF<@FGGEGD7:<6EDDECCFGGCFGGC<66,F<FFCGFC4BFF?5?< +@HWI-M00234:263:000000000-ADM55:1:1101:21334:2699 1:N:0:ATCACG +CTTTTGACGGCCTTTTTGTTGTCTTCTGAATTTTGTTTTTTTCGGTTGTAACATAATTCCTAAAAATCAAATTCGTTAGCGATTATTTTTCTTTCTTTTGAAGTTCTTTCCGCCATTGTTTCCGCTATTGCTTCCACGACC ++ +CCCCCGFGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGG@FGGGGGGGGEADGGGGGGGGGGGGGFGGGGFF@CFFEGGDGGGGGFGGGGGGGGFGFCFGGGGGFGCE=FGFDGFGGCEEGGGGDFGGGFGGGGGC +@HWI-M00234:263:000000000-ADM55:1:1101:13968:2885 1:N:0:ATCACG +CCCACTGCTGCCTCCCGTAGGAGCCTGGACCGTGTCTCAGTTCCAATGTGGCCGATCACCCTCTCAGGTCGGCTACCCATCGTTGCCCTGGTAAGCCATTACCCTACCAACTAGCTAATGGGACGCGGGCACATCTCAAAGCGGATTACCCCTTTGGTTAAAGTTTAATGCGAAACTCTAACTTTATGCGTTATTAACCCTCCTTTCGGAGGGCTATTCCCCTCTTTGATGCAGGTTGCCCACGTGTTACTCACCCGTCCGCCGCTAATCCACTTCCCGAAGGCAGATTCAACCCTCGAT ++ +<CC8CEFDGGFDF<,6@F7FCGF,:FFCED@@@,;;,<CFFFA,6C@FC@,<E+87FFCFCGFF8,<<FG7+@F7FEFFGGGCFECF,=<B=EF99F@9<FAFFGDF?=E:=4:,9,,CF9A4,>8>C+>3BFGD;@,7>,==>6?=9,,@+@4<=699C9,@+@<+72;@5;0*9:?82BF85=@2=C############################################################################################################### +@HWI-M00234:263:000000000-ADM55:1:1101:11873:2948 1:N:0:ATCACG +CCCCGTTACATCTTCCGCGCAGGAAGACTCGATCAGTGAGCTATTACGCTTTCTTTGAAGGATGGCTGCTTCTAAGCCAACTTCCTGACTGTCTTAGCCTTCCCACTTCGTTTTCCACTTAGTCAATATTAGGGACCTTAGCCGGCGGTCTGGGTTGTTTCCCTCTTGAGTCCGGACGTTAGCACCCGGTGCTCTGTCCCCCGTGCCCAAACTTCCAAGTATTCGGAGTTTGCCATGGTTTGGTAAGACGCTATGTCCCCCTAACCATAACAGTGTTCTACCCTCTGAACACATACTCGA ++ +CCCCCCD;B6C,;<EAFECF@FGGGGAECEG@F@FGGGFAAF9FGGCF>F7<FCFF<6FEFFFCDCFGGGEC<F,<A<EFC@F9BFGE<9DFFGEEGG@FFGFFGF8FDEFC<F,94:EF9,C=EGGGCCFCCE>B+>E9FCGG6+@CC>EFC8D>CEF,E=@=E?C,6=AFG>>9?DCGC>FG>GGCDA5*;+=AFF*A;/?B)-)(;64:)>3<E<3))7<E3=;>FDF?E<<4)3;EF@FFA####################################################### +@HWI-M00234:263:000000000-ADM55:1:1101:7855:3066 1:N:0:ATCACG +CGTCAATCTTCCAACGCCCACGGCAGATAGGGACAAAACTGTCTCACGACGTTTTAAACCCAGCTCACGTACCTCTTTAAATGGCGAACAGCCATACCCTTGGGACCGGCTACAGC ++ +CCCCCGGGGGGGGFGGGCBCFGGGGGFGGGGGGGGGGGGGGGG,CEGGGGGGGGGGGGGG@F@FGGGGGGGG@@EGFGGGFGFGCGEGGGGGGGGGGCFGGGGEGEEE7FFGFFGD +@HWI-M00234:263:000000000-ADM55:1:1101:24817:3238 1:N:0:ATCACG +CCCGTGACCAGATTCATGGCCGCCGGTTAGAACCCCAGTACTGTCAGGGTGGTATCCCAAGGA ++ +CCCCC7FGFGEGGGGGGDFGGGGGGDGGGGGGGGGGGGGGGGGGGGGFEECGGGGGGFFGGGG +@HWI-M00234:263:000000000-ADM55:1:1101:17179:3527 1:N:0:ATCACG +GTGCTTATTTCACTTCCTCAAAATCAGCATCCTGCACATTATCGCCATGCTTT ++ +CCCCCG<FCCGGFGGFGGGGGGGGFGGGGGGDEG?<ECGCFEG<EGGGF,CFG +@HWI-M00234:263:000000000-ADM55:1:1101:21254:3571 1:N:0:ATCACG +GTCGCGGGGAAGATCAACTCCTGCAATTCGAGCCATACCAATTACCTCCTAGCGCCCTGACGCTGTTTATGACGAGGATCGCGGCTGCAGATGACGCGAATGACGCCATTGCGCTTGATCACTCGGCAATACTCACAAATGGGTTTAACAGATTATCTAACCTTCATAGTTTGCACACTCCTCATGGACATTCC ++ +CCCCCFEFGGGGGGGGGGGGGGGGGGGGGGGGGGGG<FCCGGFFGGDFGGGFGGCGG7FGGGGGCGGAFFEEGC:FGGGGGGGGGDFGGFAGFGGGG7FEGGGGGGE7FGGG>EGGGFFFCFGGFCEEGGFGCGGGFGFDGFFGGCFGGG7C;,5=EF,CG59DCCG@,5CFFFG=FC*CFFGGGDFGGAFFFF +@HWI-M00234:263:000000000-ADM55:1:1101:16569:3709 1:N:0:ATCACG +CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGTGGAATACTTATTGCGTTTGCTGCGGCACCGAATGGCTTTGCCACCCGACACCTAGTATTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCCG ++ +CCCCCGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGG9FGGGGDGGGGGGGECGGGGGEGCGGGGGGGDGGGFGGGGGFGGGGGGFGGGGGGGGGGGACFGGGFGGGGGFGGGGGCFGGGGGGFFFFFFEF:65CFFF8FEF7AFEFEE(,6 +@HWI-M00234:263:000000000-ADM55:1:1101:22403:3828 1:N:0:ATCACG +CCCGGACATCTTCGGCGCAGGATCTCTTGACTAGTGAGCTATTACGCACTCTTTAAATGAGTGGCTGCTTCTAAGCCAACATCCTAGTTGTCTTAGAAATCCCACATCCTTTTCCACTTAACTTACACTTTGGGACCTTAGCTGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATATCAATCAAACACACACACACCAGCTTAC ++ +CCCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGEGGGGGGGGFGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGCGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG>DGGGGGGGGFDFFEDGGGCEEECGGGFGGGGGGFGGGGGGGFFFDGGGGFFGGG=D?DFFFF?CFFF5*:<EFE>+*3886=AAA@################################## +@HWI-M00234:263:000000000-ADM55:1:1101:7508:4067 1:N:0:ATCACG +GGTGCACTAGGATCGTAGTTGGCTACTTTCCCGTTTTCAATGTATACGCAAGGTACACGGTCAGCGGTAGCTGCCATAATGTAATGGTAGTCAAATCCTAAGTCTCCCAATGCACATGGCAGTGGAGCGTTCCAATCTTGTGTACCGGCTTTGTCTCCTAATCCCAAGTGCCATTTTCCGATAGCTCCGGTAGCATAACCGGTACTTTTGAACATAACCGCCATGGTATATTGTTCGGGACGGATAATCATTCCGGCATTTCCGGCAGCTACG ++ +CCCCCGFGGF8FFECFFCGE9FFGGGG9CFAFFCC@@CFGFGGCGFGGGGGGFFGDGEFFEFG8FGGF@@FGCFGGCA@FCFFCFGGGGFFGFF9??FFFFGGGGGFFF=FGGGDEF:F8,4@ACEE8CCEEGGGCCFG9FFFAFG6CGGEGE,=3EGC2;=EFFFGFGFGG9@9EGGGGC>8=DCFGGDFEFF+=7<AFFE>>5AAEF8AFE589*)849>524=;8*198?CE?9>59@>6(66@C<.))/5;>(13=EFE60(334:AB? +@HWI-M00234:263:000000000-ADM55:1:1101:26232:4161 1:N:0:ATCACG +CTCTTCTTAAGCTCGAACAGCTTCTTCAGTCTTACCTGCCATTTCATCTAAAATTTTTAAAGCTCGCTCTAAAACAGTAGTATCATCAATCATAACCAATCCACCATCTGGTCCAGGTTCCAAGTGAATCCCAACACTTTTCCCCTCCTTAAAATTGTGTCCACCGAAAAAATTTCTTACAGTGTCAACATTCAGTCCAAGTTCATCGGCTATTCTATGCATACTACCACTAGGCAATGAGT ++ +CCCCCGGFGFFFGGGGGGCFGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCFGGFGEGFGGGGFGFGFGGGFAFDGCFGGGGFGGGFGCGFGGGGCGGGFGGGG:FFGGF@F9FGGGGGFGGGGGGCEGGC?FGGGGGGGFGGDFFGF8DFGGCGGGGCDFGGGGGGGGDFGGCFGFDF7;EFFFFFFFB<FDFFAEDFFFFFF@CEFCCEEFFFFFBEFE@8=@657> +@HWI-M00234:263:000000000-ADM55:1:1101:23151:4189 1:N:0:ATCACG +GCGGATTTTCTCGGGAGTATGATTACCCACACTATTGGATTCTTCCGAAGAAGACTCCATACTACCAAGTTCAGCTCGGATGGTGGATTTGCCTGCCAAGATCAGAAGAGCACACGTCTGAACTCCAGTCACATCACGACTTCGTATGCCGTATTCTGCTTGAAAAAAACAATGAAAACTAGTCACCGATGACTCACGTTCGCTGAACATTATCTGACAACTAGCTTAACAATGGACATGCTTAGCACTGACAACCAGACG ++ +CCC8AF,6,FGGCFEEBFGCAFGGGFGGF7FB8,CF,<,;,CEE<,@,8+,,6,,,CFFGGGGG,F<EA@F,B<F6@FC6@CBE9F7F,5:F9AF84A,ABFF,=A,A,4,4AEFEGGGGGD8=EC,,4,C;EF9,9=+4++,+4=8DGG6@############################################################################################################# +@HWI-M00234:263:000000000-ADM55:1:1101:17148:4803 1:N:0:ATCACG +CCCTTTTCGGCAATGGCGATGTTACGGTACATAAGAGCGATTTCGGGGAAACCTTCTTCGTCTGCAATATCAGCAAACTTAGGATAATCCAATGA ++ +AACBCDFA@@:+:CCEF7@CC@@AFFDGD;FDA@@6E@FF<,CC;@@+7@FG@F<CCFF@F@F@,<,B@F,CDG<@FEF<@,@,CAF9E<FD<?? +@HWI-M00234:263:000000000-ADM55:1:1101:8939:4808 1:N:0:ATCACG +CCACAGTTCTGTCATATCGCAGTACAGGAATCTCAACCTGTTGTCCATCGGCTACGGCTCTCGCCCTCGCCTTAGGCCCCGACTTACCCAGGGCAGATCAGCTTTA ++ +CC8C9C6<EFGFEEGGGGGGGDECGFDE@FFAFGFGFGGGGGGGGFGFEE@FFGFEGGCFECDGCCFGC+6@B@@FFG,8F@FFGGGGFEDFCF@FFFGGDFFDFF +@HWI-M00234:263:000000000-ADM55:1:1101:11727:5103 1:N:0:ATCACG +CTCCCGTATAGGATGGATCGAAAGCAATCGTCAGGAATCCACGTTCTGCCAATGTCTGCGCATACAAGCCCGAAGCTTGTTCCTTCACGGCACCAAATGGGCCACTGATGGCAATGGCAGGCAATTTACCCGTCACATTCTTGGGTACATACAAATCGGCGGCAAGTGTAATGCCGTAACGGTTGTGGAAAG ++ +CCCCCGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFFGGGGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFFGGGGGGGGGGGGGGFGGFDF@GGDGGGGGGGGG4*;CFFF +@HWI-M00234:263:000000000-ADM55:1:1101:15029:5173 1:N:0:ATCACG +GGCCTATTCTCTGCGGCCGCCTTTCGGCGGCACCCCTTCTCCCGAAGTTACGGGGTCATTTTGCCGAGTTCCTTAACAATGCTTCTTCCGTCGGCCTTAGGATTCTCTCCTCATCCACCTGTGTCGGTTTACGGTACGGGTACAATATAAACAATAGCGGCTTTTCTTGACGGCCGGCTCATGGGCTTCCCTACTTTAGT ++ +CCCCCGGGGGGGGGGGGGGGGGGGGEGG7FECGFGGGGGEGGGGGEEGGGGGGGGGGGGGGGFGGGGGGGGGGGGFGGGCGGGGGGGGGGGGGGGFFGGGGGGGGGGFFG9FCGGGFGGGGGFFFDGGEECGGGGGGGGGGGGGGGGGGGGECGGGGGD>CCG>=98E79=CDDDD4D5DGF=FGGGDGFFFFFFFF6AC +@HWI-M00234:263:000000000-ADM55:1:1101:7330:5248 1:N:0:ATCACG +TGTGTATGAATGAGCGGCTACAGGCTTAGTCGATTTCTTTACTGCGCAGAAGCGGGACTGTGACCGACAAAACAGCCGTCGCCTCTCTACGTTCCGGGACCATACGCCGCTCGCAGTGATTGACCCTTACAACCTTGCGGGCGCTCTC ++ +CCCCCGEGGGGGGFGGGGGEGGGGGGFGGGGGGGGGGGGGGGGGGGGGGGFFGGGGDEGGGGGFGGGGGGGGGGECGGGGEFGDGAFGGGGGGFFG>GGECFFGCF+@CC@FGG++@FFFF,>F,DFFGGFFGCGEFG6CEG54*=@D +@HWI-M00234:263:000000000-ADM55:1:1101:18630:5353 1:N:0:ATCACG +GGCGTGTTCTGCCAAAATAGTGCTGGGCAATATACGACATAGAAGCGAAAGGCAATACTTTTTCTAAGGCCTGCCTGATTTCTACTGTTTTCACAATGGCTTGGGCTTCATTGATTGATTGCCTGGCACCATCTTGGAATGCTTGCGCAAACGCTTTTTTATCCTCCGGTGAAAGCGTCTGCAGAAAAGCCCTGAAACGTTTTTTATGGTCGGCCACTTCCTCAGGAGTATTG ++ +CCCCCGGGGGGGGGCGGGGGGGGGGFGGFGFGGGGGGGEGGGGGGGGGGGGGDECDFGGGGGGGGGGFGGGGDGGGGGGGGGGGGFFFGGGGGGGGGGGGGGGGGD8<FGGGFGGGGGGGFAEGGFGC,8EFGGGGFDC9DG9E9DCEE>EGGDGGFGGGGFG?FCFDED6F6DG6C6==DD7=D=D6DFF?F**3;9@?699CDBFF5FEB>>D>:@00>=C*8:;25;59* +@HWI-M00234:263:000000000-ADM55:1:1101:11799:5476 1:N:0:ATCACG +CCTAAATTTTCGAGTATAAGCAGGACCGAAGCCACCAACTTATACCTTATCCTCTAAATTTTCGTAAGAGACATGAAGCGGATCTCTAACTATTCCCGATTTTACTGCACCACTTGATCAAACGCTTCGGAACAAGAGCTTTTGAGTGATGTCTCGTTTCCACCACTTTGGCAGAAATAAAGCTAATCTACTATGATTCGATTAAGCAGCAAGAGCATAATTTTCGTTGCCAGATAAAATTT ++ +CCCC<FFFGGF@FFFGFFGCGCCFGGGEFGGD;CFFGFGGGGGGCFF@FFGGC<EEGGGCEFGGGF@E@@,BFFGFGGGGG7+@AFEAEFFGGGGGGGFFEGGFGGGCEGEFDGFGGFGG:=>@EC@@77@E>4DEC8C>DGGG>FGGGGF=DAECDDFDEG8==++=,6=FGDGFGGGGGGGGGGFFGFFFFFFFFFFDFFADFFFEAF@F@C5@EEF5=E;EE4477AFBEFBF;*A4>C +@HWI-M00234:263:000000000-ADM55:1:1101:11348:5668 1:N:0:ATCACG +GCCTAAATTTTCGTGCAGCGGCAGAACCAAAGCCATCAACCGTTGCCTTATCCTCTAAATTTTCACCCGCGCCACGAGGCTGACACGAACTATCCCCGATTTGACTGCACCACTTTATCGGAATGCTTCGGAGCAACAGCTTCCGAGTGATGTCACGTCCCAGCACCTGGTGCCGGGATTAAGCTTCAATCTACTATACTTCGATTAAGCAGCGAGAGCGTAACGAGTTTCGCCAGATAAAATTTTGAGGACTGAGATTAAAGTGAAAATC ++ +CCCCCGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCFGFGGGGGDGGCGGGGGGGGGGGGGGFGGGGGGGGGGGGGGGFGGGGGGDGGGGGGGGGDGGG6>E9CCGGGGGGGGGGGGGGGGGGFGFGFFFFFFFFFFFFFFAFFFFFFFFFFFB199@F=9@DF@?;@FDFFFFFF>274A>B<?FABFF7?F???FB94:<<F26)-69>0 +@HWI-M00234:263:000000000-ADM55:1:1101:25874:5826 1:N:0:ATCACG +CCTGCAGTGGATAGGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGTACCTCTTTAATGGGCGAACAGCCCAACCCTTGGGACCTTCTCCAGCCCCAGGATGAGATGAGCCGACATCGAGGTGCCAAACTTTCCCGCCGATATGGACGCCCGGGCAAAATCAGCTTGTTATCCCCAGGGTAGCTTTTATCCGTTGAGCGACGACCCTTCCATTCGGAATCGCCGGAATACTATGCCCTGCTTTCGCACCTGCCCAACCAGTCCGCCCAGCAGTTAAG ++ +CCCCCDFGGGG<@F,CE<FGGEGDDFG9EEEF7CGFGGG9<E@D@@EC,@FFFEGGG,C<9C<FGGFGF+@CGEECGEFFGGGG<F<A,,4?F?,?5EDF,,=BEF+,A<A,C,,AE84+F=+F>FFGGFFGD8D>DE,,373++++++6,,3@+++@9*@9>5@C*<::7,*0;CC7+:*39CF*045?8AC=A:BB:5)05/;6:6)86::8@F#################################################################### +@HWI-M00234:263:000000000-ADM55:1:1101:26932:5861 1:N:0:ATCACG +AGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGTGTTCTTCCTAATCTCTACGCATTTCACCGCTACACTAGGAATTCCGCTTTCCTCTCCTGCACTCTAGATATCCAGTTTGGAATGCAGCCCCCAGGTTAAGCCCGGGGATTTCACATCCCACTTAAACATCCGCCTACGCACCCTTTACCACCAGTAAATCCGGACAACGCTCGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCCTCTGGTACCTTCATTATCGTCCCCGAAACC ++ +96BCCFGGEGGGFGGGGG?EFFGGGGGCFG9FDFGGFG8FGGCFGGGGGGGGGFDGGDFEBFGFFGGGGGEEEFGGG<FFFCFCFFGDCFGFGG<8FGEFGGGCCFFAEGGFFGFFFFGG8EEFCEGG@EDEGGFGFFFFGGFCBEE8CF8=CFFAF,=D8F8FGG+@DC86?=CGFDGC9D6FC:FC*+;9*331?+=;ABB49>FDEB=45@@FFFBFF;8@E@FC)/6690(-1)),82?B??B################################################ +@HWI-M00234:263:000000000-ADM55:1:1101:14154:5990 1:N:0:ATCACG +GTGGGATGTCAAGGTAAGCGGCGGCAGCCTTGGCTACAGCAAGAGATACGCCGAGGATGGCATTGGCACCCAGATTGGCTTTCGTTTTGGTTCCGTCCAGTGCAAGCATGGCATGGTCGATACCCATCTGGTCGAGGGCGGACATACCGATGAGCTTGGGAGCAATGATATTATTGATATTGTCCACGGCTTTCAGGAC ++ +CCCCCGGC<F9DE@EG9FF@@FGFGGFEFGF@E@FAFGGGFGFEE8FFEGGGGD>>=F8FG8?EEDFGGGGGG<FFD?FF@FFGE8F=,>FFEBCEFGGGFGGGGGGCEG8D?FFGGEGGFFEGEE8FFGGGG8++>8E>E5@BDA?FGGGGGGCFF6E==57??86ECFCF@CCCCBFCGDGGGG=*>3)868:7=FF +@HWI-M00234:263:000000000-ADM55:1:1101:25022:6229 1:N:0:ATCACG +CCCGAGAACGTATTCACCGCAACATTGCTGATTTGCGATTACTAGTGATTCCAACTTCATGTACTCGAGTTGCAGAGTACAATCCGAACTACGAACAGCTTTCTGAGGTTTGCTCCTCCTCGCAGATTTGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC ++ +CCCCCGGGGGGFGGGFGGGGEGGCFGFGGAEGGGDGGEGGGFGFFGG9FFGEFGGGGGGGGGAEGGG8FFGGG?EFGGGGCFGGGCCFGFFGCFGGGCGGGGGG9EF,EGGCFFGFFGGEEGGGDEGGGAFGGGCEFEEBE8EDFGGGGGGGGCGGGGGGGGFGCFDD +@HWI-M00234:263:000000000-ADM55:1:1101:21023:6286 1:N:0:ATCACG +GTGTACAAGGCCCGGGAACGTATTCACCGCGCCGTGGCTGATGCGCGATTACTAGCGAATCCAGCTTCATGGAGTCGGGTTGCAGACTCCAATCCGAACTGAGAGAGGCTTTAGGGATTAGCATCACGTCGCCGTGTAGCTGCCTTCTGTACCCCCCATTGTAACACGTGTGTAGCCCCGGACGTAAGGGCCGTGCTGATTTGCCGTCATCCCCACCTTCCTCAC ++ +CCCCCGGGCFFGGDEGGGGGGFGGGGGGGGGGGGGEGGGGGFEGGGGGGGDFFG9FCGGGGGGGGGGGFFFGGGGGEGCFGDGGGAG8EF<FFGGGGGGGFGGCDGDEGFGGGGGGGFGF?GGDFDFGDDEGD+B=DFFFD9DGFGGGGGEGCFCEGEGGGGFCF>FGGCGGGGCGGFDDG<55CF4CFF;5555>FFFFFA6+0(2,08)00(36,,)5:AB2: +@HWI-M00234:263:000000000-ADM55:1:1101:8138:6336 1:N:0:ATCACG +TGTGTACAAGGCCCGGGAACGTATTCACCGCGACATTCTGATTCGCGATTACTAGCAACTCCAGCTTCATGTAGGCGAGTTGCAGCCTACAATCCGAACTGAGATAGGTTTTATAAGTTTTGCTCCACCTCACGGTCTT ++ +CCCCCGGGF<FGGGGED@FGGGFGGGGGGDGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGFGGGCGGGGGGGGGGGGGFGGFEGGGGDGGEFCCGEFFGGGGAFGGGGGFGFFFGGFGGFGDFGGGGGG +@HWI-M00234:263:000000000-ADM55:1:1101:11055:6543 1:N:0:ATCACG +CCTGGACATTTTCGGCGCAGGACCTCCCGACCAGTGAGCTATTACGCACTCTTTGACTGTGTGGCTGCTTCTGAGCCAACATCCTGGTTGACTTTGAAATCTCACATACTTTTCCACTTAACACGCACTTTGGGACCTTAGCTGGAGGTCTTGGCTCTTTCCTTTTTGCCTATCCAACTTATCTCGTATAGTCTGACTCCCGG ++ +@B,8BF8EFGFGG77@7+8@@6,FFF<,+@C+6FF99@A@EC,CC,BFFDFGA<@D,C9F@CF7,B7CFGG,C@,B@<F?7FF8<,:,C@,?<EE,C?A,E,ACEFG9,C;@,,A;EEA,,?E9884B8=,9,4,@+7>@,B;=,66+@D,,@,3=EF=:@F,C=886,+6@D8D+6+0==C9+6+5+0;+1+3BC1<A?9>9 +@HWI-M00234:263:000000000-ADM55:1:1101:24980:6575 1:N:0:ATCACG +GCCATTTATCTACGCCTGTTGTCACCAACAGGCTCTAGCGGTCTACCCTCCGACGTGGGGCGAGCAACCCTCATA ++ +CCCCCGGGGGFGGGGGGGGGGGFGGGGGGGG@G<FFFGGGEEGGGGGGFGGCECFGGGGGD:@FGGGGG7ADGGG +@HWI-M00234:263:000000000-ADM55:1:1101:7596:6682 1:N:0:ATCACG +GCCCCCGTCAATTCCTTTGAGTTTCACCGTTGCCGGCGTACTCCCCAGGTGGAATACTTAACGCTTTCGCTTGGCGGCTGACAATGTATCGCCAACCGCGAGTATTCATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCCGATCTCGTATGCCGTCCTCGGCTTGAAAAAAAAAAATATAAACCATGATGAATTTCGAACACTCACGACCACACACTAAGCGTCGACGTA ++ +CCCCCGGGGGGACFGGGGGFFEEGGGGGGFFEGGGGGGGGGGEGFGGGGEFG@FGDFFGGGGGGGEGGGDGFFCC>FGCDFGFGGGCE,E:FCCCC7CGGGGGGFE;FFEFCCGFGG@CEFGGGG@F@>@8DGGG=;@FFFGGC;E6,@@+4:,318;9,@FG@########################################################################### +@HWI-M00234:263:000000000-ADM55:1:1101:24419:6736 1:N:0:ATCACG +CGGGCGTTGGCAAGTACGTTATATCACTTACCCACGCTCGATTCAGAACCCCAGGAGTAAATGCTCGTTTTAAGAGGTTGGGATACACAGGCATGGTGTGATTACTGTCGGTGGTTTTTACGGTTCGCTTAAAAAAGCGGTAACCAATGATGCCATTAAAGCGAAGGATAGCGCGTAAACGTTTGACACCAACTTTCACTCCACGGTGTTGCATCAAAGC ++ +CCCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDGGGGGFGGGGGGGDGGGGGGGGGGGGGGGGDFGGGGGGGGGGGGGGGGGGGGGGGGFGGGFGGGGGGGGD9FFCFEGGGFGGGGG7=+4>@EFEEFFG7>EEDEEEFDDDG@8DCFEFDFGGC8=CDCGGFGGDC6>DDGDCFDGDGDGFGG@AFBA?FFFFFFFFABBF95B@AFFFFFFFF +@HWI-M00234:263:000000000-ADM55:1:1101:5068:6755 1:N:0:ATCACG +GCAGCGGCAGAACCAAAGCCATCAACCGTTGCCTTATCCTCTAAATTTTCACCCGCGCCACGAGGCTGACACGAACTATCCCCGATTTGACTGCACCACTTTATCGGAATGCTTCGGAGCAACAGCTTCCGAGTGATGTCACGTCCCAGCACCTGGTGCCGGGATTAAGCTTCAATCTACTATACTTCGATTAAGCAGCGAGAGCGTAACGAGTTTCGCCAGATAAAATTTTGAGGACTGAGATTAAAGTGCAAATC ++ +CCCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGDFF?FGGGGGDFGGGGGGGGGGFFFFGGGGGFGGFGGGGGGDGGGGGGGFGGGEEDGGG9C9EEGGGGGD8@FGGGGGGGGGGGGG>CFGGGGGCFGDGDFGFGGF7CFGDF>FFFAF=;A>EDDBFBBFFF@D@D<E?@F01;@<?C<471.2)48<??2(549<B>FDF24)66<)) +@HWI-M00234:263:000000000-ADM55:1:1101:21477:6796 1:N:0:ATCACG +CCCCACTGCTGCTTCCCGTAGGAGTTTGGACCGTGTCTCAGTTCCAATGTGGGGGACCTTCCTCTCAGAACCCCTATCCATCGTTGACTAGGTGGGCCGTTACCCCGCCTACTATCTAATGGAACGCATCCCCATCGTCTACCGGAAAATACCTT ++ +CC,AC:F8<F@CCEDGGCEGGGGGFAG<FGG8@FEFEFFCEGGCFF<<<F@<C+@FF:AFCEFE,ECF9AFGC@DE<FE,,5@9B8BFFCE8F,=FF@CG7FCFGD:FFF7EAFDG9F;FFF@:FEEG@4=@DEFG6=EGCADC6@ECCA,@DDF +@HWI-M00234:263:000000000-ADM55:1:1101:12483:6880 1:N:0:ATCACG +CTCCTTGTCTGCCTTCGATTACGGCATTTGCAATCGTTCCTGTAAGTAGTTTTACAGCTCTAATGGCATCGTCATTCCCTGGTATTACGTAGTCTATTTCATCAGGATCACAGTTAGTATCAACTATTGCTACAACTGGTATTCCTAATATCTTCGCTTCATTTACTGCAATTCTTTCTTTTCTTGGATCTACAACAAATAATACATCAGGAAGACCACCCATATCTTGAATGCCACCTAAAAACTTTTCAA ++ +CCCCCGGGGGGGFGGFGGGGGFGDFEGGGGGFFFGGFGGGGGGGGFGGGFGGGGGGGGCEGGGGCFGGGGGGGGGGGGFGGGFEGGDGGGGFFFGGGGGGGGFGGGGGGGGGGFGFGCF@FEFFGDAGFGFGGGGFGGGGDGGGFFGGGD;EEFGGGGGGGFGGGGGGGGGGFDGDGGGGGGGGGGGDGFFFFFFF9;;DFFFFFBFFBDBFFBFD=DDFDFFFEFEFEFEECE<@E@CFF@EBAFFEEEFF +@HWI-M00234:263:000000000-ADM55:1:1101:27958:6935 1:N:0:ATCACG +CCATTCGGAAATCCGCGGATCAAAGGTCATTTGCACCTACCCGCAGCTTATCGCAGCTTATCACGTCCTTCATCGCCTCCGAGAGCCAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCCACAAATTCACACTACACACCCACCTCCCATCACGCATCTCTTTTGTCCGAGTCACGCTGCACGCTACCTGCACACTACCTGCCTCAGTTACATCTTTTTACTCAATGTCCCACCATTTATCAC ++ +CCCCCGGGGGGGGGGGDGGCFGGGGGGGFEGGGGGGGGGGGGGGGGGGGGGGGGGGG8DC<FGGGFFGGGGGGGGGGCGF:FDGGGGGG@FEEEGGGEGDGGGFGGGGGG:FFGG=EGFFFCEFG9EEFGD+@@EEDED8DA>EEC@EFGFAFFGF=F?+44;C############################################################################################################################## +@HWI-M00234:263:000000000-ADM55:1:1101:6151:7404 1:N:0:ATCACG +GCACGTAGTTAGCCGGGGCTTCCTCCTAAGGTACCGTCATTATCGTCCCTTAGGACAGAGCTTTACGATCCGAAAACCTTCATCGCTCACGCGGCGTCGCTGCATCAGAGTTCCCTCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCCGTTCACCCTCCCAGGCCGGCTACCCATCGTCGCCTTGGTTAGCTATTACCTCCCCAACTAGCTAATGGGACGCGAGTCCAGCTTATACCACTTAACCGCTTTTGCTTGAAAATCA ++ +CCCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGFGFGGGGGGGGGGFGGGGGGGGGGGGGGGGGGDF8FGGGFGGGGEGGG7+CGGFGGGGGGGGC:8FGGGDFGFEGGGFGGGFGCGGGGGGGGDG?EGGGGGFF,<FGGGGFDGGGG,5,EFGGGD++*5:DGFCDD?6>AFFF4?6)5?EB?D0>DFFFAF0(/.6<D03;3().646<A)).))(4:0<BFBD?F<2<AFF<B############################################ \ No newline at end of file