Mercurial > repos > artbio > small_rna_map
changeset 8:2cc2948cfa34 draft default tip
planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_map commit b6c6b4d92a8d839e59f7ac28cb169d25ca68ef0d
author | artbio |
---|---|
date | Sun, 23 Jul 2017 13:54:29 -0400 |
parents | 35d3f8ac99cf |
children | |
files | small_rna_map.py small_rna_map.xml test-data/coverage.pdf test-data/doubled.tab test-data/doubled_coverage.pdf test-data/doubled_mean.pdf test-data/doubled_median.pdf test-data/mean.pdf test-data/median.pdf test-data/output.tab test-data/sizes.pdf |
diffstat | 11 files changed, 25 insertions(+), 4859 deletions(-) [+] |
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--- a/small_rna_map.py Sun Jul 23 05:21:58 2017 -0400 +++ b/small_rna_map.py Sun Jul 23 13:54:29 2017 -0400 @@ -40,19 +40,24 @@ Returns a map_dictionary {(chromosome,read_position,polarity): [read_length, ...]} ''' - map_dictionary = dict() + map_dictionary = defaultdict(list) + # get empty value for start and end of each chromosome for chrom in self.chromosomes: - for pos in range(self.chromosomes[chrom]): - map_dictionary[(chrom, pos+1, 'F')] = [] # get all chromosomes - map_dictionary[(chrom, pos+1, 'R')] = [] # get all chromosomes + map_dictionary[(chrom, 1, 'F')] = [] + map_dictionary[(chrom, self.chromosomes[chrom], 'F')] = [] for chrom in self.chromosomes: for read in bam_object.fetch(chrom): - read_positions = read.positions # a list of covered positions + positions = read.positions # a list of covered positions + for pos in positions: + if not map_dictionary[(chrom, pos+1, 'F')]: + map_dictionary[(chrom, pos+1, 'F')] = [] + if not map_dictionary[(chrom, pos+1, 'R')]: + map_dictionary[(chrom, pos+1, 'R')] = [] if read.is_reverse: - map_dictionary[(chrom, read_positions[-1]+1, + map_dictionary[(chrom, positions[-1]+1, 'R')].append(read.query_alignment_length) else: - map_dictionary[(chrom, read_positions[0]+1, + map_dictionary[(chrom, positions[0]+1, 'F')].append(read.query_alignment_length) return map_dictionary @@ -100,21 +105,25 @@ def compute_coverage(self, map_dictionary, quality=10): ''' - Takes a map_dictionary and returns a dictionary of lists - of coverage along the coordinates of pre_mirs (as keys) + takes a map_dictionary as input and returns + a coverage_dictionary {(chromosome,read_position,polarity): + coverage} ''' coverage_dictionary = dict() for chrom in self.chromosomes: + coverage_dictionary[(chrom, 1, 'F')] = 0 + coverage_dictionary[(chrom, self.chromosomes[chrom], 'F')] = 0 + + for key in map_dictionary: coverage = self.bam_object.count_coverage( - reference=chrom, - start=0, - end=self.chromosomes[chrom], + reference=key[0], + start=key[1]-1, + end=key[1], quality_threshold=quality) """ Add the 4 coverage values """ coverage = [sum(x) for x in zip(*coverage)] - for pos, cov in enumerate(coverage): - coverage_dictionary[(chrom, pos+1, "F")] = cov - coverage_dictionary[(chrom, pos+1, "R")] = cov + coverage_dictionary[key] = coverage[0] + # coverage_dictionary[(key[0], key[1], 'R')] = coverage return coverage_dictionary def compute_size(self, map_dictionary):
--- a/small_rna_map.xml Sun Jul 23 05:21:58 2017 -0400 +++ b/small_rna_map.xml Sun Jul 23 13:54:29 2017 -0400 @@ -1,4 +1,4 @@ -<tool id="small_rna_map" name="small rna map" version="0.6.0"> +<tool id="small_rna_map" name="small rna map" version="0.7.0"> <description></description> <requirements> <requirement type="package" version="1.11.2=py27_0">numpy</requirement> @@ -74,24 +74,12 @@ <output file="median.pdf" name="output_pdf" /> </test> <test> - <param name="input" value="input1.bam,input1.bam" ftype="bam"/> - <param name="extra_plot" value="Median" /> - <output file="doubled.tab" name="output_tab" /> - <output file="doubled_median.pdf" name="output_pdf" /> - </test> - <test> <param name="input" value="input1.bam,input2.bam" ftype="bam"/> <param name="extra_plot" value="Coverage" /> <output file="output.tab" name="output_tab" /> <output file="coverage.pdf" name="output_pdf" /> </test> <test> - <param name="input" value="input1.bam,input1.bam" ftype="bam"/> - <param name="extra_plot" value="Coverage" /> - <output file="doubled.tab" name="output_tab" /> - <output file="doubled_coverage.pdf" name="output_pdf" /> - </test> - <test> <param name="input" value="input1.bam,input2.bam" ftype="bam"/> <param name="extra_plot" value="Sizes" /> <output file="output.tab" name="output_tab" />
--- a/test-data/doubled.tab Sun Jul 23 05:21:58 2017 -0400 +++ b/test-data/doubled.tab Sun Jul 23 13:54:29 2017 -0400 @@ -105,44 +105,7 @@ input1.bam_0 FBtr0070001 72 52 0 R 27 0 0 40 input1.bam_0 FBtr0070001 72 53 0 F 27 0 0 39 input1.bam_0 FBtr0070001 72 53 0 R 27 0 0 39 -input1.bam_0 FBtr0070001 72 54 0 F 27 0 0 0 -input1.bam_0 FBtr0070001 72 54 0 R 27 0 0 0 -input1.bam_0 FBtr0070001 72 55 0 F 27 0 0 0 -input1.bam_0 FBtr0070001 72 55 0 R 27 0 0 0 -input1.bam_0 FBtr0070001 72 56 0 F 27 0 0 0 -input1.bam_0 FBtr0070001 72 56 0 R 27 0 0 0 -input1.bam_0 FBtr0070001 72 57 0 F 27 0 0 0 -input1.bam_0 FBtr0070001 72 57 0 R 27 0 0 0 -input1.bam_0 FBtr0070001 72 58 0 F 27 0 0 0 -input1.bam_0 FBtr0070001 72 58 0 R 27 0 0 0 -input1.bam_0 FBtr0070001 72 59 0 F 27 0 0 0 -input1.bam_0 FBtr0070001 72 59 0 R 27 0 0 0 -input1.bam_0 FBtr0070001 72 60 0 F 27 0 0 0 -input1.bam_0 FBtr0070001 72 60 0 R 27 0 0 0 -input1.bam_0 FBtr0070001 72 61 0 F 27 0 0 0 -input1.bam_0 FBtr0070001 72 61 0 R 27 0 0 0 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--- a/test-data/output.tab Sun Jul 23 05:21:58 2017 -0400 +++ b/test-data/output.tab Sun Jul 23 13:54:29 2017 -0400 @@ -105,44 +105,7 @@ input1.bam FBtr0070001 72 52 0 R 27 0 0 40 input1.bam FBtr0070001 72 53 0 F 27 0 0 39 input1.bam FBtr0070001 72 53 0 R 27 0 0 39 -input1.bam FBtr0070001 72 54 0 F 27 0 0 0 -input1.bam FBtr0070001 72 54 0 R 27 0 0 0 -input1.bam FBtr0070001 72 55 0 F 27 0 0 0 -input1.bam FBtr0070001 72 55 0 R 27 0 0 0 -input1.bam FBtr0070001 72 56 0 F 27 0 0 0 -input1.bam FBtr0070001 72 56 0 R 27 0 0 0 -input1.bam FBtr0070001 72 57 0 F 27 0 0 0 -input1.bam FBtr0070001 72 57 0 R 27 0 0 0 -input1.bam FBtr0070001 72 58 0 F 27 0 0 0 -input1.bam FBtr0070001 72 58 0 R 27 0 0 0 -input1.bam FBtr0070001 72 59 0 F 27 0 0 0 -input1.bam FBtr0070001 72 59 0 R 27 0 0 0 -input1.bam FBtr0070001 72 60 0 F 27 0 0 0 -input1.bam FBtr0070001 72 60 0 R 27 0 0 0 -input1.bam FBtr0070001 72 61 0 F 27 0 0 0 -input1.bam FBtr0070001 72 61 0 R 27 0 0 0 -input1.bam FBtr0070001 72 62 0 F 27 0 0 0 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