changeset 8:2cc2948cfa34 draft default tip

planemo upload for repository https://github.com/ARTbio/tools-artbio/tree/master/tools/small_rna_map commit b6c6b4d92a8d839e59f7ac28cb169d25ca68ef0d
author artbio
date Sun, 23 Jul 2017 13:54:29 -0400
parents 35d3f8ac99cf
children
files small_rna_map.py small_rna_map.xml test-data/coverage.pdf test-data/doubled.tab test-data/doubled_coverage.pdf test-data/doubled_mean.pdf test-data/doubled_median.pdf test-data/mean.pdf test-data/median.pdf test-data/output.tab test-data/sizes.pdf
diffstat 11 files changed, 25 insertions(+), 4859 deletions(-) [+]
line wrap: on
line diff
--- a/small_rna_map.py	Sun Jul 23 05:21:58 2017 -0400
+++ b/small_rna_map.py	Sun Jul 23 13:54:29 2017 -0400
@@ -40,19 +40,24 @@
         Returns a map_dictionary {(chromosome,read_position,polarity):
                                                     [read_length, ...]}
         '''
-        map_dictionary = dict()
+        map_dictionary = defaultdict(list)
+        # get empty value for start and end of each chromosome
         for chrom in self.chromosomes:
-            for pos in range(self.chromosomes[chrom]):
-                map_dictionary[(chrom, pos+1, 'F')] = []  # get all chromosomes
-                map_dictionary[(chrom, pos+1, 'R')] = []  # get all chromosomes
+            map_dictionary[(chrom, 1, 'F')] = []
+            map_dictionary[(chrom, self.chromosomes[chrom], 'F')] = []
         for chrom in self.chromosomes:
             for read in bam_object.fetch(chrom):
-                read_positions = read.positions  # a list of covered positions
+                positions = read.positions  # a list of covered positions
+                for pos in positions:
+                    if not map_dictionary[(chrom, pos+1, 'F')]:
+                        map_dictionary[(chrom, pos+1, 'F')] = []
+                    if not map_dictionary[(chrom, pos+1, 'R')]:
+                        map_dictionary[(chrom, pos+1, 'R')] = []
                 if read.is_reverse:
-                    map_dictionary[(chrom, read_positions[-1]+1,
+                    map_dictionary[(chrom, positions[-1]+1,
                                     'R')].append(read.query_alignment_length)
                 else:
-                    map_dictionary[(chrom, read_positions[0]+1,
+                    map_dictionary[(chrom, positions[0]+1,
                                     'F')].append(read.query_alignment_length)
         return map_dictionary
 
@@ -100,21 +105,25 @@
 
     def compute_coverage(self, map_dictionary, quality=10):
         '''
-        Takes a map_dictionary and returns a dictionary of lists
-        of coverage along the coordinates of pre_mirs (as keys)
+        takes a map_dictionary as input and returns
+        a coverage_dictionary {(chromosome,read_position,polarity):
+                                                coverage}
         '''
         coverage_dictionary = dict()
         for chrom in self.chromosomes:
+            coverage_dictionary[(chrom, 1, 'F')] = 0
+            coverage_dictionary[(chrom, self.chromosomes[chrom], 'F')] = 0
+
+        for key in map_dictionary:
             coverage = self.bam_object.count_coverage(
-                                                reference=chrom,
-                                                start=0,
-                                                end=self.chromosomes[chrom],
+                                                reference=key[0],
+                                                start=key[1]-1,
+                                                end=key[1],
                                                 quality_threshold=quality)
             """ Add the 4 coverage values """
             coverage = [sum(x) for x in zip(*coverage)]
-            for pos, cov in enumerate(coverage):
-                coverage_dictionary[(chrom, pos+1, "F")] = cov
-                coverage_dictionary[(chrom, pos+1, "R")] = cov
+            coverage_dictionary[key] = coverage[0]
+            # coverage_dictionary[(key[0], key[1], 'R')] = coverage
         return coverage_dictionary
 
     def compute_size(self, map_dictionary):
--- a/small_rna_map.xml	Sun Jul 23 05:21:58 2017 -0400
+++ b/small_rna_map.xml	Sun Jul 23 13:54:29 2017 -0400
@@ -1,4 +1,4 @@
-<tool id="small_rna_map" name="small rna map" version="0.6.0">
+<tool id="small_rna_map" name="small rna map" version="0.7.0">
   <description></description>
   <requirements>
         <requirement type="package" version="1.11.2=py27_0">numpy</requirement>
@@ -74,24 +74,12 @@
             <output file="median.pdf" name="output_pdf" />
         </test>
         <test>
-            <param name="input" value="input1.bam,input1.bam" ftype="bam"/>
-            <param name="extra_plot" value="Median" />
-            <output file="doubled.tab" name="output_tab" />
-            <output file="doubled_median.pdf" name="output_pdf" />
-        </test>
-        <test>
             <param name="input" value="input1.bam,input2.bam" ftype="bam"/>
             <param name="extra_plot" value="Coverage" />
             <output file="output.tab" name="output_tab" />
             <output file="coverage.pdf" name="output_pdf" />
         </test>
         <test>
-            <param name="input" value="input1.bam,input1.bam" ftype="bam"/>
-            <param name="extra_plot" value="Coverage" />
-            <output file="doubled.tab" name="output_tab" />
-            <output file="doubled_coverage.pdf" name="output_pdf" />
-        </test>
-        <test>
             <param name="input" value="input1.bam,input2.bam" ftype="bam"/>
             <param name="extra_plot" value="Sizes" />
             <output file="output.tab" name="output_tab" />
Binary file test-data/coverage.pdf has changed
--- a/test-data/doubled.tab	Sun Jul 23 05:21:58 2017 -0400
+++ b/test-data/doubled.tab	Sun Jul 23 13:54:29 2017 -0400
@@ -105,44 +105,7 @@
 input1.bam_0	FBtr0070001	72	52	0	R	27	0	0	40
 input1.bam_0	FBtr0070001	72	53	0	F	27	0	0	39
 input1.bam_0	FBtr0070001	72	53	0	R	27	0	0	39
-input1.bam_0	FBtr0070001	72	54	0	F	27	0	0	0
-input1.bam_0	FBtr0070001	72	54	0	R	27	0	0	0
-input1.bam_0	FBtr0070001	72	55	0	F	27	0	0	0
-input1.bam_0	FBtr0070001	72	55	0	R	27	0	0	0
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-input1.bam_0	FBtr0070001	72	57	0	R	27	0	0	0
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-input1.bam_0	FBtr0070001	72	58	0	R	27	0	0	0
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-input1.bam_0	FBtr0070001	72	59	0	R	27	0	0	0
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-input1.bam_0	FBtr0070001	72	67	0	R	27	0	0	0
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-input1.bam_0	FBtr0070001	72	69	0	R	27	0	0	0
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-input1.bam_0	FBtr0070001	72	70	0	R	27	0	0	0
-input1.bam_0	FBtr0070001	72	71	0	F	27	0	0	0
-input1.bam_0	FBtr0070001	72	71	0	R	27	0	0	0
 input1.bam_0	FBtr0070001	72	72	0	F	27	0	0	0
-input1.bam_0	FBtr0070001	72	72	0	R	27	0	0	0
 input1.bam_0	FBtr0070533	72	1	1	F	5	15.0	15.0	1
 input1.bam_0	FBtr0070533	72	1	0	R	5	0	0	1
 input1.bam_0	FBtr0070533	72	2	0	F	5	0	0	1
@@ -253,40 +216,7 @@
 input1.bam_0	FBtr0070533	72	54	0	R	5	0	0	4
 input1.bam_0	FBtr0070533	72	55	0	F	5	0	0	4
 input1.bam_0	FBtr0070533	72	55	0	R	5	0	0	4
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-input1.bam_0	FBtr0070533	72	59	0	F	5	0	0	0
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-input1.bam_0	FBtr0070533	72	60	0	F	5	0	0	0
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-input1.bam_0	FBtr0070533	72	71	0	F	5	0	0	0
-input1.bam_0	FBtr0070533	72	71	0	R	5	0	0	0
 input1.bam_0	FBtr0070533	72	72	0	F	5	0	0	0
-input1.bam_0	FBtr0070533	72	72	0	R	5	0	0	0
 input1.bam_0	FBtr0070603	72	1	2	F	9	16.5	16.5	2
 input1.bam_0	FBtr0070603	72	1	0	R	9	0	0	2
 input1.bam_0	FBtr0070603	72	2	0	F	9	0	0	2
@@ -430,7 +360,6 @@
 input1.bam_0	FBtr0070603	72	71	0	F	9	0	0	1
 input1.bam_0	FBtr0070603	72	71	0	R	9	0	0	1
 input1.bam_0	FBtr0070603	72	72	0	F	9	0	0	0
-input1.bam_0	FBtr0070603	72	72	0	R	9	0	0	0
 input1.bam_0	FBtr0070604	72	1	1	F	24	15.0	15.0	1
 input1.bam_0	FBtr0070604	72	1	0	R	24	0	0	1
 input1.bam_0	FBtr0070604	72	2	0	F	24	0	0	1
@@ -461,10 +390,6 @@
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 input1.bam_0	FBtr0070604	72	15	0	F	24	0	0	1
 input1.bam_0	FBtr0070604	72	15	0	R	24	0	0	1
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@@ -537,12 +462,6 @@
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 input1.bam_0	FBtr0070604	72	53	0	F	24	0	0	31
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 input1.bam_0	FBtr0070604	72	57	1	F	24	15.0	15.0	1
 input1.bam_0	FBtr0070604	72	57	0	R	24	0	0	1
 input1.bam_0	FBtr0070604	72	58	0	F	24	0	0	1
@@ -574,35 +493,7 @@
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 input1.bam_0	FBtr0070604	72	71	0	R	24	0	0	1
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 input1.bam_0	FBtr0070911	73	16	0	F	1	0	0	1
@@ -645,10 +536,6 @@
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@@ -679,72 +566,8 @@
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 input1.bam_1	FBtr0080660	72	5	0	F	4	0	0	4
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 input1.bam_1	FBtr0080663	71	2	0	F	56	0	0	22
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 input1.bam_1	FBtr0080663	71	47	0	F	56	0	0	1
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 input1.bam_1	FBtr0080663	71	70	0	R	56	0	0	1
 input1.bam_1	FBtr0080663	71	71	0	F	56	0	0	0
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 input1.bam_1	FBtr0080664	71	1	16	F	56	19.5	17.0	16
 input1.bam_1	FBtr0080664	71	1	0	R	56	0	0	16
 input1.bam_1	FBtr0080664	71	2	0	F	56	0	0	16
Binary file test-data/doubled_coverage.pdf has changed
Binary file test-data/doubled_mean.pdf has changed
Binary file test-data/doubled_median.pdf has changed
Binary file test-data/mean.pdf has changed
Binary file test-data/median.pdf has changed
--- a/test-data/output.tab	Sun Jul 23 05:21:58 2017 -0400
+++ b/test-data/output.tab	Sun Jul 23 13:54:29 2017 -0400
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 input2.bam	FBtr0080663	71	47	0	F	22	0	0	1
 input2.bam	FBtr0080663	71	47	0	R	22	0	0	1
-input2.bam	FBtr0080663	71	48	0	F	22	0	0	0
-input2.bam	FBtr0080663	71	48	0	R	22	0	0	0
-input2.bam	FBtr0080663	71	49	0	F	22	0	0	0
-input2.bam	FBtr0080663	71	49	0	R	22	0	0	0
 input2.bam	FBtr0080663	71	50	2	F	22	15.5	15.5	2
 input2.bam	FBtr0080663	71	50	0	R	22	0	0	2
 input2.bam	FBtr0080663	71	51	1	F	22	19.0	19.0	3
Binary file test-data/sizes.pdf has changed