Takes reads in FASTA, FASTQ or SFF format and looks for the given primer sequences (within the existing clipped sequence) and further clips the reads to remove the primers and any preceding/trailing sequence. Supports forward and reverse primers, including IUPAC ambiguity codes. Note this tool was initially written for Roche 454 data, and should also work fine on Sanger or Ion Torrent as well. However, it is probably too slow for use on large Illumina datasets. |
hg clone https://testtoolshed.g2.bx.psu.edu/repos/peterjc/seq_primer_clip
Name | Version | Type | |
---|---|---|---|
biopython | 1.67 | package | |
galaxy_sequence_utils | 1.0.1 | package |
Name | Description | Version | Minimum Galaxy Version |
---|---|---|---|
Trim off 5' or 3' primers | 0.0.16 | 16.01 |