# HG changeset patch # User yhoogstrate # Date 1454925364 18000 # Node ID 209e96e870e8eb8c7642f1589c6a2c3edab72167 # Parent 4ceaf2551a79abfc3a5ebb0878106eb6cec9606c planemo upload for repository https://github.com/ErasmusMC-Bioinformatics/fuma_galaxy_wrapper commit d90385601350b4d5fafae3004b470dee4a7f442d-dirty diff -r 4ceaf2551a79 -r 209e96e870e8 fuma.xml --- a/fuma.xml Wed Dec 09 09:56:08 2015 -0500 +++ b/fuma.xml Mon Feb 08 04:56:04 2016 -0500 @@ -1,10 +1,10 @@ - + match detected fusion genes based on gene names (in particular for RNA-Seq) python - fuma + fuma fuma --version 2>&1 | head -n 1 @@ -64,12 +64,15 @@ + - + + + @@ -224,6 +227,8 @@ +-------------------+-----------------------+-------------------------------------+ |DeFuse | results.filtered.txt | defuse | +-------------------+-----------------------+-------------------------------------+ +|EricScript | .results.total.txt | ericscript ************* | ++-------------------+-----------------------+-------------------------------------+ |Fusion Catcher | final-list_cand*.txt | fusion-catcher_final | +-------------------+-----------------------+-------------------------------------+ |FusionMap | | fusionmap | @@ -234,6 +239,10 @@ +-------------------+-----------------------+-------------------------------------+ |RNA STAR | Chimeric.out.junction | rna-star_chimeric | +-------------------+-----------------------+-------------------------------------+ +|SOAPFuse | final.*.for.genes.txt | soapfuse-final-gene | ++-------------------+-----------------------+-------------------------------------+ +|SOAPFuse | final.*.for.trans.txt | soapfuse-final-transcript | ++-------------------+-----------------------+-------------------------------------+ |STAR Fusion | _candidates.final | star-fusion_final | +-------------------+-----------------------+-------------------------------------+ |TopHat Fusion pre | fusions.out | tophat-fusion_pre | @@ -245,6 +254,12 @@ |TopHat Fusion post | result.html | tophat-fusion_post_result_html | +-------------------+-----------------------+-------------------------------------+ +************* EricScript often contains entries with unknown breakpoints. +Because no genomic coordinates are given those fusion genes can not be +imported into FuMa and only those with breakpoints will be taken into account. + + + To annotate genes upon the breakpoints you must provide a BED file that contains gene annotations for the user genome build. Make sure **your BED file contains one gene per line**. You should use BED files that contain one exon per line only if you want restrict your analysis to fusion genes detected within exons. UCSC genome browser provides a very simple way of obtaining BED files with one gene per line by selecting their *RefSeq Genes*-track and *knownGene*-table and putting the export format to BED. Galaxy should have a built-in UCSC table browser. diff -r 4ceaf2551a79 -r 209e96e870e8 tool_dependencies.xml --- a/tool_dependencies.xml Wed Dec 09 09:56:08 2015 -0500 +++ b/tool_dependencies.xml Mon Feb 08 04:56:04 2016 -0500 @@ -4,6 +4,6 @@ - +