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<?xml version="1.0" encoding="UTF-8"?>
<tool id="flaimapper" name="FlaiMapper" version="1.1.5.a">
	<description>Detect small ncRNA derived fragments using Fragment Location Annotation Identification Mapper.</description>
	<requirements>
		<requirement type="package" version="0.8.2.1">pysam</requirement>
		<requirement type="package" version="1.1.5">flaimapper</requirement>
	</requirements>
	
	<version_command>flaimapper --version</version_command>
	
	<command>
		flaimapper
			 -v
			 -f $output_format
			 -o $output
			 -m $mask
			 -r $fasta
		
		#for $alignment in $alignments
			$alignment
		#end for
	</command>
	
	<stdio>
		<regex match="[fai_load] build FASTA index." source="stderr" level="log" />
	</stdio>
	
	<inputs>
		<param name="alignments" type="data" format="bam" label="Alignment file(s)" help="Aligned small RNA-Seq reads which may not be fragmented. In case you add multiple BAM files, FlaiMapper will simply concatenate the data and perform one single analysis on the entire set of alignments." multiple="true" />
		
		<param name="mask" type="data" format="gtf,gff,gff3" label="small ncRNA Annotation (gtf)" help="" />
			
		<param name="fasta" type="data" format="fasta" label="Fasta sequence corresponding to reference genome" help="" />
		
		<param name="output_format" type="select" label="Output format">
			<option value="1">Tabular (1 fragment per column)</option>
			<option value="2">Tabular (1 precursor per column)</option>
			<option value="3">GenBank</option>
			<!-- option value="gtf">GTF/GFF</option -->
		</param>
	</inputs>
	
	<outputs>
		<data format="tabular" name="output" label="${tool.name} on ${', '.join([ str(a.hid)+': '+a.name for a in $alignments ])}" />
	</outputs>
	
	<tests>
		<test>
			<param name="alignments" value="snord81.bam" ftype="bam" />
			<param name="mask" value="ncrnadb09.gtf" ftype="gtf" />
			<param name="fasta" value="ncrnadb09.fa" ftype="fasta" />
			<param name="output_format" value="1" />
			
			<output name="output" file="snord81.flaimapper.txt" />
		</test>
	</tests>
	
	<help>
FlaiMapper wrapper for Galaxy
=============================

https://github.com/yhoogstrate/flaimapper
http://www.ncbi.nlm.nih.gov/pubmed/25338717
http://dx.doi.org/10.1093/bioinformatics/btu696

Fragment Location Annotation Identification Mapper

FlaiMapper: computational annotation of small ncRNA-derived fragments using RNA-seq high-throughput data.


Input
-----

Alignments
**********

Aligned reads from small RNA-Seq experiments have to be provided in the BAM format.
In case you add multiple BAM files, FlaiMapper will simply concatenate the data and perform one single analysis on the entire set of alignments.

Mask File
*********

There are two strategies to analyze using FlaiMapper:

- Relative to mature ncRNA sequences
- Relative to chromosomes

Therefore FlaiMapper requires a list of ncRNA annotations relative to the used reference genome for the alignment files. These ncRNA locations within the sequences provided in the FASTA file (MASK) regions should be provided in the GFF/GTF format:

- http://genome.ucsc.edu/FAQ/FAQformat.html#format3
- http://www.ensembl.org/info/website/upload/gff.html

If you are making use of a ncRNA database that has no GTF file available you can make use of the galaxy tool **flaimapper-gtf-from-fasta** to create one.


You can access **ncRNAdb09** GTF file at the following URL:
https://raw.githubusercontent.com/yhoogstrate/flaimapper/master/share/annotations/ncRNA_annotation/ncrnadb09.gtf *(mask file)*

Fasta sequence
**************

The reference sequence should be provided in FASTA format.

You can access **ncRNAdb09** FASTA file at the following URL:
https://raw.githubusercontent.com/yhoogstrate/flaimapper/master/share/annotations/ncRNA_annotation/ncrnadb09.fa *(reference file)*

Example- and reference data
***************************

To align reads to ncRNA you need aligner specific indexed version of the reference. We have made
the following available for ncRNAdb09:

 - **Tophat1**: https://github.com/yhoogstrate/flaimapper/blob/master/share/annotations/ncRNA_annotation/ncrnadb09.bt2.tar.gz
 - **Tophat2**: https://github.com/yhoogstrate/flaimapper/blob/master/share/annotations/ncRNA_annotation/ncrnadb09.bt2.tar.gz

If you want to test FlaiMapper with example data you can obtain several
alignment files from the following directory tree:

https://github.com/yhoogstrate/flaimapper/tree/master/share/small_RNA-seq_alignments

Installation
------------

The wrapper makes use of easy_install to install a python egg. Please
ensure you have easy_install installed.

License
-------

**flaimapper** and **wrapper**:

GPL (>=3)

**pysam**:

The MIT License

Contact
-------

The tool wrapper has been written by Youri Hoogstrate from the Erasmus
Medical Center (Rotterdam, Netherlands).


Development
-----------

* Repository-Maintainer: Youri Hoogstrate
* Repository-Developers: Youri Hoogstrate

* Repository-Development: https://bitbucket.org/EMCbioinf/galaxy-tool-shed-tools

The tool wrapper has been written by Youri Hoogstrate from the Erasmus
Medical Center (Rotterdam, Netherlands).

	</help>
	
	<citations>
		<citation type="doi">10.1093/bioinformatics/btu696</citation>
	</citations>
</tool>