# HG changeset patch # User yating-l # Date 1486073205 18000 # Node ID ad9a212044051db527db88bb79cbf5d2170ff217 # Parent 34c6f02de27f45563a6d9db09d941496b1322bbc planemo upload commit 46cd27d5fb1b88220fb94b9039a685359b87d539 diff -r 34c6f02de27f -r ad9a21204405 README.rst --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.rst Thu Feb 02 17:06:45 2017 -0500 @@ -0,0 +1,57 @@ +Galaxy wrapper for UCSC pslCDnaFilter tool (version: v340) +========================================================== +Filter cDNA alignments in psl format. Filtering criteria are comparative, selecting near best in genome alignments for each given cDNA and non-comparative, based only on the quality of an individual alignment. + +parameters: +----------- +pslCDnaFilter parameters variable definitions by genome assembly category and type of cDNA sequence. + +# common paramters used for all assembly categories + +Same species: + -minQSize=20 -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap -polyASizes + +Across species: + -minQSize=20 -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap -polyASizes + +# common paramters used for local near best filtering + +Same species: + -localNearBest=0.001 +Across species: + -localNearBest=0.010 + +# finished assemblies + +Same species: + -minId=0.95 -minCover=0.25 +Across species: + -minId=0.35 -minCover=0.25 + +# well-ordered assemblies + +Same species: + -minId=0.95 -minCover=0.15 +Across species: + -minId=0.35 -minCover=0.15 + +# low-coverage assemblies + +Same species: + -minId=0.94 -minAlnSize=80 +Across species: + -minId=0.33 -minAlnSize=80 + +Reference: +++++++++++ +`Configuration file for GenBank RefSeq alignment process `_ + + +Source code: +------------- + +http://hgdownload.cse.ucsc.edu/admin/exe/ + +Licence +------- +Please note that commercial download and installation of the Blat and In-Silico PCR software may be licensed through Kent Informatics (http://www.kentinformatics.com).