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1 <h1 id="conversion-of-ncbi-blast-tblastn-results-to-psl-format">Conversion of NCBI BLAST+ tblastn results to PSL format</h1>
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2 <p>Wilson Leung <script type="text/javascript">
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3 <!--
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4 h='wustl.edu';a='@';n='wleung';e=n+a+h;
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5 document.write('<a h'+'ref'+'="ma'+'ilto'+':'+e+'" clas'+'s="em' + 'ail">'+e+'<\/'+'a'+'>');
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6 // -->
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7 </script><noscript>wleung at wustl dot edu</noscript></p>
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8 <p>Last Update: 04/24/2016</p>
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9 <h2 id="version-information">Version information</h2>
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10 <ul>
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11 <li>Kent source tree: v324</li>
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12 <li>NCBI BLAST+: BLAST 2.2.30+</li>
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13 </ul>
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14 <h2 id="data-sources">Data sources</h2>
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15 <p>For testing purposes, the database consists of only contig1 in the Dbia3 assembly while the protein sequences correspond to the three isoforms of the <em>D. melanogaster</em> <em>ci</em> gene in contig1. The protein sequences are available through <a href="http://flybase.org/cgi-bin/getseq.html?source=dmel&id=FBgn0004859&chr=4&dump=PrecompiledFasta&targetset=translation">FlyBase</a>.</p>
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16 <ul>
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17 <li>Dbia3.fa = contig1 sequence in the Dbia3 asssembly</li>
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18 <li>ci.pep = Protein sequences for the three isoforms of the <em>ci</em> gene in <em>D. melanogaster</em></li>
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19 </ul>
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20 <h2 id="conversion-protocol">Conversion protocol</h2>
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21 <ol style="list-style-type: decimal">
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22 <li><p>Create BLAST database for the assembly</p>
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23 <pre><code>makeblastdb -in Dbia3.fa -dbtype nucl</code></pre></li>
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24 <li><p>Perform tblastn search and output results in XML format</p>
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25 <pre><code>tblastn -outfmt 5 -db Dbia3.fa -query ci.pep -out tblastn_Dbia3_ci.xml -evalue 1e-2</code></pre></li>
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26 <li><p>Convert results into PSL format</p>
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27 <pre><code>blastXmlToPsl -convertToNucCoords tblastn_Dbia3_ci.xml tblastn_Dbia3_ci.xml.psl</code></pre></li>
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28 <li><p>Convert PSL output into BED format</p>
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29 <pre><code>pslToBed tblastn_Dbia3_ci.xml.psl tblastn_Dbia3_ci.xml.bed</code></pre></li>
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30 </ol>
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31 <h2 id="output-files">Output files</h2>
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32 <ul>
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33 <li>tblastn_Dbia3_ci.xml = tblastn results in XML format</li>
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34 <li>tblastn_Dbia3_ci.xml.psl = tblastn results in PSL format</li>
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35 <li>tblastn_Dbia3_ci.xml.bed = tblastn results in BED format</li>
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36 </ul>
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