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1 <tool id="QDNAseq" name="QDNAseq" version="0.0.1">
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3 <requirements>
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4 <requirement type="set_environment">QDNASEQ_SCRIPT_PATH</requirement>
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5 <requirement type="package" version="3.0.3">R</requirement>
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6 <requirement type="package" version="1.0.4">bioc_qdnaseq</requirement>
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7 </requirements>
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8
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9 <description>Quantitative copy number abberation detection</description>
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10
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11 <command interpreter="Rscript">
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12 QDNAseq.R
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13 $qdnaseq_cfg <!-- use a tmp config file to pass all params to R by source() -->
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14 </command>
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15
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16 <stdio>
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17 <!-- Anything higher than 0 means the R script didnt finish -->
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18 <!-- Because different R packages deal with err/warn differently unable to waterproof this -->
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19 <exit_code range="1:" level="fatal" description="R script didnt finish correctly, check log" />
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20 </stdio>
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21
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22 <inputs>
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23 <!-- ==================== -->
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24 <!-- General inputs -->
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25 <!-- ==================== -->
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26 <param name="jobName" type="text" optional="false" label="Analysis/ouput name" help="Supply a name for the outputs to remind you what they contain" value="TEST">
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27 <validator type="empty_field" />
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28 <validator type="regex" message="This field should contain some non-whitespace character">.*\S</validator>
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29 <!-- <validator type="expression" message="Window Size must be even">value % 2 ==0</validator> -->
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30 </param>
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31 <param name="binSize" type="select" label="Select bin-size to use (kb)" help="Larger bin sizes provide faster analysis but lower resolution">
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32 <option value="1000">1Mb</option>
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33 <option value="100">100kb</option>
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34 <option value="30">30kb</option>
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35 <option value="15">15kb</option>
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36 <option value="5">5kb</option>
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37 <option value="1">1kb</option>
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38 </param>
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39 <param name="doCall" type="select" label="Also segment and call with CGHcall" help="This setting will be set to yes if called output is requested (see additional history outputs)">
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40 <option value="TRUE">yes</option>
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41 <option value="FALSE">no</option>
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42 </param>
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43 <param name="experimentType" type="select" label="Type of sequencing data" help="Currently only single end reads of lenght 50 are supported within galaxy">
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44 <option value="SR50">Single Read 50bp</option>
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45 <!-- <option value="PE1000">PairedEnd1000</option> -->
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46 </param>
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47
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48 <!--
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49 <param name="excludeChrs" type="select" multiple="true" label="Exclude certain Chromosomes">
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50 <option value="X">X</option>
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51 <option value="Y">Y</option>
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52 </param>
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53 -->
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54
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55 <!-- ==================== -->
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56 <!-- Input BAMs -->
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57 <!-- ==================== -->
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58 <param name="bams" type="data" multiple="true" optional="false" format="bam" label="Input BAMs" help="Select all BAM files to include in the analysis" />
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59
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60 <!-- ==================== -->
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61 <!-- This section contains galaxy history output settings -->
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62 <!-- ==================== -->
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63 <conditional name="extra_history_outputs">
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64 <param name="show" type="select" label="Show additional history outputs">
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65 <option value="NO">Only output Report to history</option>
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66 <option value="YES">Select additional history outputs</option>
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67 </param>
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68 <when value="YES">
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69 <param name="readcounts_rds" type="select" label="Also output readcounts RDS (R object) to history">
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70 <option value="FALSE">no</option>
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71 <option value="TRUE">yes</option>
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72 </param>
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73 <param name="copynumbers_rds" type="select" label="Also output copynumbers RDS (R object) to history">
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74 <option value="FALSE">no</option>
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75 <option value="TRUE">yes</option>
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76 </param>
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77 <param name="calls_rds" type="select" label="Also output called segments RDS (R object) to history">
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78 <option value="FALSE">no</option>
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79 <option value="TRUE">yes</option>
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80 </param>
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81 </when>
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82 <when value="NO">
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83 <param name="readcounts_rds" type="hidden" value="FALSE" />
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84 <param name="copynumbers_rds" type="hidden" value="FALSE" />
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85 <param name="calls_rds" type="hidden" value="FALSE" />
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86 </when>
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87 </conditional>
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88
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89 <!-- ==================== -->
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90 <!-- Option to use your own bin annotations -->
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91 <!-- ==================== -->
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92 <conditional name="use_own_binannotation">
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93 <param name="show" type="select" label="Use your own bin annotations from history">
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94 <option value="no">no</option>
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95 <option value="yes">yes</option>
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96 </param>
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97 <when value="yes">
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98 <param name="binannotation_file" type="data" multiple="false" format="rds" label="R data structure file with bin-annotations" help="If you made your own bin-annotations with the QDNAseq bioconductor package you can upload them to your history and select here" />
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99 </when>
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100 <when value="no">
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101 <param name="binannotation_file" type="hidden" value="" />
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102 </when>
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103 </conditional>
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104 <!-- ==================== -->
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105 <!-- Optional advanced options -->
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106 <!-- ==================== -->
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107 <conditional name="advanced">
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108 <param name="show" type="select" label="Show advanced options">
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109 <option value="no">no</option>
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110 <option value="yes">yes</option>
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111 </param>
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112 <when value="yes">
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113
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114 <param name="undo_splits" type="select" label="undoSplits" help="If set to sdundo, see undoSD below">
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115 <option value="sdundo">sdundo</option>
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116 <option value="prune">prune</option>
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117 <option value="none">none</option>
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118 </param>
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119
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120 <param name="undoSD" size="10" type="float" value="1" label="undoSD" help='The number of SDs between means to keep a split if undo.splits="sdundo".' />
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121
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122 <param name="blacklist" type="select" label="Filter blacklisted bins (blacklist)" help="Will exclude all blacklisted bins in the binannotation if set">
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123 <option value="TRUE">yes</option>
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124 <option value="FALSE">no</option>
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125 </param>
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126
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127 <param name="mappability" type="integer" value="0" min="0" max="100" label="Filter bins with lower mappability" help="Will exclude all bins will lower mappability than this number (0-100)" />
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128
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129 <param name="debug" type="select" label="DEBUG" help="Uses the inbuilt LGG data instead of input BAMs">
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130 <option value="FALSE">no</option>
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131 <option value="TRUE">yes</option>
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132 </param>
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133 </when>
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134 <!-- need to set defaults because params are passed to R anyway -->
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135 <when value="no">
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136 <param name="undoSD" type="hidden" value="1" />
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137 <param name="undo_splits" type="hidden" value="sdundo" />
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138 <param name="blacklist" type="hidden" value="TRUE" />
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139 <param name="mappability" type="hidden" value="0" />
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140 <param name="debug" type="hidden" value="FALSE" />
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141 </when>
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142 </conditional>
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143
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144 <!-- ==================== -->
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145 <!-- Optional graphical/plotting options -->
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146 <!-- ==================== -->
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147 <conditional name="plot_options">
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148 <param name="show" type="select" label="Show graphical options">
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149 <option value="no">no</option>
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150 <option value="yes">yes</option>
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151 </param>
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152 <when value="yes">
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153 <param name="plot_width" size="3" type="integer" value="960" label="Plot width" />
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154 <param name="plot_height" size="3" type="integer" value="480" label="Plot height" />
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155 <param name="exclude_chrs" type="select" multiple="true" label="Hide these chromosomes in plots" help="Currently only standard human chromosomes supported. NOTE: other filters might also exclude chromosomes">
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156 <option value="1">1</option>
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157 <option value="2">2</option>
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158 <option value="3">3</option>
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159 <option value="4">4</option>
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160 <option value="5">5</option>
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161 <option value="6">6</option>
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162 <option value="7">7</option>
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163 <option value="8">8</option>
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164 <option value="9">9</option>
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165 <option value="10">10</option>
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166 <option value="11">11</option>
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167 <option value="12">12</option>
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168 <option value="13">13</option>
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169 <option value="14">14</option>
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170 <option value="15">15</option>
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171 <option value="16">16</option>
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172 <option value="17">17</option>
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173 <option value="18">18</option>
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174 <option value="19">19</option>
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175 <option value="20">20</option>
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176 <option value="21">21</option>
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177 <option value="22">22</option>
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178 <option value="X" selected="true">X</option>
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179 <option value="Y" selected="true">Y</option>
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180 </param>
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181 </when>
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182 <when value="no">
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183 <param name="plot_width" type="hidden" value="960" />
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184 <param name="plot_height" type="hidden" value="480" />
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185 <param name="exclude_chrs" type="hidden" value="X,Y" />
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186 </when>
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187 </conditional>
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188 </inputs>
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189 <!-- ==================== -->
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190 <!-- This config is sourced in R code -->
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191 <!-- ==================== -->
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192 <configfiles>
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193 <configfile name="qdnaseq_cfg">
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194 ## this file was sourced in QDNAseq R wrapper script
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195 ## in this way all galaxy params are passes to R
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196
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197 ## required params
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198 as.integer( "${binSize}" ) -> binSize
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199 "${experimentType}" -> experimentType
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200 "${jobName}" -> outputName
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201
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202 ## extra params
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203 as.logical( "${doCall}" ) -> doCall
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204 "${htmlFile}" -> outputHtml
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205 "${htmlFile.files_path}" -> outputPath
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206 "${use_own_binannotation.binannotation_file}" -> binAnnotations
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207
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208 ## advanced options
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209 as.double( "${advanced.undoSD}" ) -> undoSD
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210 as.logical( "${advanced.debug}" ) -> debug
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211 as.logical( "${advanced.blacklist}" ) -> filterBlacklistedBins
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212 as.integer( "${advanced.mappability}" ) -> mappabilityCutoff
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213 "${advanced.undo_splits}" -> undoSplits
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214
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215 ## history output params
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216 as.logical( "${extra_history_outputs.readcounts_rds}" ) -> doOutputReadcountsRds
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217 as.logical( "${extra_history_outputs.copynumbers_rds}" ) -> doOutputCopynumbersRds
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218 as.logical( "${extra_history_outputs.calls_rds}" ) -> doOutputCallsRds
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219 "${rdsReadCounts}" -> readCountsDatasetFile
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220 "${rdsCopyNumbers}" -> copyNumbersDatasetFile
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221 "${rdsCalls}" -> calledSegmentsDatasetFile
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222
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223 ## plotting params
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224 as.integer( "${plot_options.plot_width}" ) -> PLOT_WIDTH
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225 as.integer( "${plot_options.plot_height}" ) -> PLOT_HEIGHT
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226 "${plot_options.exclude_chrs}" -> excludeChrsString
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227
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228 ## input BAMs init
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229 c() -> bamsPaths
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230 c() -> bamsNames
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231
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232 #for bam in $bams#
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233 c( bamsPaths, "${bam}" ) -> bamsPaths
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234 c( bamsNames, "${bam.name}" ) -> bamsNames
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235 #end for
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236
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237 </configfile>
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238 </configfiles>
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239
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240 <!-- ==================== -->
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241 <!-- Main output is an html based report, additional on request -->
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242 <!-- ==================== -->
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243 <outputs>
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244
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245 <data format="html" name="htmlFile" label="QDNAseq Report ${binSize}kb (${jobName})" />
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246
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247 <data format="rds" name="rdsReadCounts" label="${jobName}_readCounts_${binSize}kb.rds">
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248 <filter> extra_history_outputs['readcounts_rds'] == "TRUE" </filter><!-- <filter>("readcounts_rds" in outputs)</filter> -->
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249 </data>
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250
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251 <data format="rds" name="rdsCopyNumbers" label="${jobName}_copyNumbers_${binSize}kb.rds">
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252 <filter> extra_history_outputs['copynumbers_rds'] == "TRUE" </filter>
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253 </data>
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254
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255 <data format="rds" name="rdsCalls" label="${jobName}_calls_${binSize}kb.rds">
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256 <filter> extra_history_outputs['calls_rds'] == "TRUE" </filter>
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257 </data>
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258
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259 </outputs>
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260
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261 <!-- ==================== -->
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262 <!-- Tests still to be done -->
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263 <!-- ==================== -->
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264
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265 <!--
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266 <tests>
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267 <test>
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268 <param name="input1" value="5.bed" />
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269 <param name="distance" value="1" />
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270 <param name="minregions" value="2" />
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271 <param name="returntype" value="1" />
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272 <output name="output" file="gops-cluster-1.bed" />
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273 </test>
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274 </tests>
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275 -->
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276 <!-- <requirements>
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277 <requirement type="package">ucsc_tools</requirement>
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278 </requirements>
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279 -->
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280
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281 <help>
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282 .. class:: infomark
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283
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284 **Introduction**
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285
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286 This tool is a wrapper for the R Bioconductor package QDNAseq_
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287
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288 .. _QDNAseq: http://www.bioconductor.org/packages/release/bioc/html/QDNAseq.html
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289
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290 It determines the copy number state of human chromosomes 1 - 22 for (shallow coverage) whole genome sequencing data.
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291
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292 -----
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293
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294 .. class:: warningmark
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295
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296 The input BAMs are expected to be **single end reads of 50bp length** mapped to GRCh37/hg19 genome build. Other experiment setups are currently not supported within galaxy. See the documentation of QDNAseq at bioconductor on how to deal with different setups.
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297
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298 .. class:: warningmark
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299
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300 Requires **internet access** for downloading bin-annotations from bitbucket and to show css styling of the final report
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301
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302 .. class:: warningmark
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303
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304 If the data is noisy, a **larger binsize** should be chosen
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305
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306 -----
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307
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308 **Example**
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309
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310 To be done
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311
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312 -----
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313
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314 **Citation**
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315
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316 For the underlying tool please cite: llari Scheinin, Daoud Sie et al. DNA copy number analysis of fresh and formalin-fixed specimens by whole-genome sequencing: improved correction of systematic biases and exclusion of problematic regions, (submitted). See also the bioconductor package_ documentation.
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317
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318 .. _package: http://www.bioconductor.org/packages/release/bioc/html/QDNAseq.html
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319 </help>
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320
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321 </tool> |
