# HG changeset patch # User stef # Date 1419866730 18000 # Node ID f1e90e523e676f87c1d149e802fc07c6372fdbe5 # Parent 3f55530dedd00fddcc93a27e7342ccaa781239f9 Deleted selected files diff -r 3f55530dedd0 -r f1e90e523e67 falcoCall.xml --- a/falcoCall.xml Mon Dec 29 10:24:12 2014 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,133 +0,0 @@ - - - - - samtools - R - - - - - FALCO: Amplicon Analysis Pipeline - - - falco-call.sh $falco_cfg; - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - \S+ - - - - - - - - - - - - - - - - - - filter_file=$filter_file - manifest_file=$manifest_file - bam_file=$bam - bam_name=$bam.name - html_out=$html_output - vcf_out=$vcf_output - out_path=${html_output.files_path} - ## reference source - REF_SOURCE="${genomeSource.refGenomeSource}" - #if $genomeSource.refGenomeSource == "history": - ##build index on the fly - REF_FILE="${genomeSource.ownFile}" - DB_KEY="${dbkey}" - #else: - ##use precomputed indexes - REF_FILE="${genomeSource.indices.fields.path}" - #end if - - - - - - - - - - - - - - - - - - -.. class:: infomark - -**Introduction** - -This tool is a wrapper for the variant caller FALCO_ which is part of the Amplicon Analysis Pipeline (AAP) - -.. _FALCO: https://github.com/tgac-vumc/falco/ - -Calls and annotates genomic variants for each amplicon in a design. - -**Notes** - -Because each amplicon is considered separate test, if two amplicons overlap this can cause the same variant position to be present twice in the output VCF. This is intentional, you can use this to evaluate the quality of the variant call beyond the amplicon. - ------ - -**Citation** - -For the underlying tool please cite: Daoud Sie et al. Performance of amplicon-based next generation DNA sequencing for diagnostic gene mutation profiling in oncopathology (Cell Oncol 2014 Oct;37(5):353-61). [Pubmed] - -.. _Pubmed: http://www.ncbi.nlm.nih.gov/pubmed/25209392 - - - -