# HG changeset patch # User stef # Date 1419866620 18000 # Node ID 150c2182f77ec44b21923f6894d4f66281c70ab4 # Parent c8b7098539a7aae16a72e2919dfc8fb2385cee38 Deleted selected files diff -r c8b7098539a7 -r 150c2182f77e falco-call.xml --- a/falco-call.xml Mon Dec 29 09:01:35 2014 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,133 +0,0 @@ - - - - - samtools - R - - - - - FALCO: Amplicon Analysis Pipeline - - - falco-call.sh $falco_cfg; - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - \S+ - - - - - - - - - - - - - - - - - - filter_file=$filter_file - manifest_file=$manifest_file - bam_file=$bam - bam_name=$bam.name - html_out=$html_output - vcf_out=$vcf_output - out_path=${html_output.files_path} - ## reference source - REF_SOURCE="${genomeSource.refGenomeSource}" - #if $genomeSource.refGenomeSource == "history": - ##build index on the fly - REF_FILE="${genomeSource.ownFile}" - DB_KEY="${dbkey}" - #else: - ##use precomputed indexes - REF_FILE="${genomeSource.indices.fields.path}" - #end if - - - - - - - - - - - - - - - - - - -.. class:: infomark - -**Introduction** - -This tool is a wrapper for the variant caller FALCO_ which is part of the Amplicon Analysis Pipeline (AAP) - -.. _FALCO: https://github.com/tgac-vumc/falco/ - -Calls and annotates genomic variants for each amplicon in a design. - -**Notes** - -Because each amplicon is considered separate test, if two amplicons overlap this can cause the same variant position to be present twice in the output VCF. This is intentional, you can use this to evaluate the quality of the variant call beyond the amplicon. - ------ - -**Citation** - -For the underlying tool please cite: Daoud Sie et al. Performance of amplicon-based next generation DNA sequencing for diagnostic gene mutation profiling in oncopathology (Cell Oncol 2014 Oct;37(5):353-61). [Pubmed] - -.. _Pubmed: http://www.ncbi.nlm.nih.gov/pubmed/25209392 - - - -