Mercurial > repos > stef > falco
diff README.rst @ 80:ebb4c3b8b9db draft
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| author | stef |
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| date | Fri, 20 Mar 2015 08:58:17 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/README.rst Fri Mar 20 08:58:17 2015 -0400 @@ -0,0 +1,24 @@ +FALCO Amplicon Analysis Pipeline & Galaxy wrapper +======================================== + +FALCO variant-caller is part of the Amplicon Analysis Pipeline (AAP). + +The typical workflow is as follows: +* paired-end amplicon sequencing +* merge pairs (so only overlapping pairs are included) +* map the single read fastq with BWA +* perform variant calling with FALCO +* create (html) report of the results + +FALCO uses samtools and straight-forward statistics to determine wether a +potential variant is likely a (technical) artifact or not. + +Input / Output + +Input of the caller is a BAM file, output VCF + + +Bug Reports & other questions +============================= + +Issues can be reported via http://www.tgac.nl/
