changeset 0:980bf1f6f37b draft default tip

Uploaded
author slegras
date Mon, 27 Jul 2015 08:14:06 -0400
parents
children
files subtract_query.py subtract_query.xml
diffstat 2 files changed, 239 insertions(+), 0 deletions(-) [+]
line wrap: on
line diff
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/subtract_query.py	Mon Jul 27 08:14:06 2015 -0400
@@ -0,0 +1,113 @@
+#!/usr/bin/env python
+# Greg Von Kuster
+
+"""
+Subtract an entire query from another query
+usage: %prog in_file_1 in_file_2 begin_col end_col output
+    --ignore-empty-end-cols: ignore empty end columns when subtracting
+"""
+import sys, re
+from galaxy import eggs
+import pkg_resources; pkg_resources.require( "bx-python" )
+from bx.cookbook import doc_optparse
+
+# Older py compatibility
+try:
+    set()
+except:
+    from sets import Set as set
+
+assert sys.version_info[:2] >= ( 2, 4 )
+
+def get_lines(fname, begin_col='', end_col='', ignore_empty_end_cols=False):
+    lines = set([])
+    i = 0
+    for i, line in enumerate(file(fname)):
+        line = line.rstrip('\r\n')
+        if line and not line.startswith('#'):
+            if begin_col and end_col:
+                """Both begin_col and end_col must be integers at this point."""
+                try:
+                    line = line.split('\t')
+                    line = '\t'.join([line[j] for j in range(begin_col-1, end_col)])
+                    if ignore_empty_end_cols:
+                        # removing empty fields, we do not compare empty fields at the end of a line.
+                        line = line.rstrip()
+                    lines.add( line )
+                except: pass
+            else:
+                if ignore_empty_end_cols:
+                    # removing empty fields, we do not compare empty fields at the end of a line.
+                    line = line.rstrip()
+                lines.add( line )
+    if i: return (i+1, lines)
+    else: return (i, lines)
+
+def main():
+    
+    # Parsing Command Line here
+    options, args = doc_optparse.parse( __doc__ )
+
+    try:
+        inp1_file, inp2_file, begin_col, end_col, out_file = args
+    except:
+        doc_optparse.exception()
+    
+    begin_col = begin_col.strip()
+    end_col = end_col.strip()
+    
+    if begin_col != 'None' or end_col != 'None':
+        """
+        The user selected columns for restriction.  We'll allow default
+        values for both begin_col and end_col as long as the user selected
+        at least one of them for restriction.
+        """
+        if begin_col == 'None':
+            begin_col = end_col
+        elif end_col == 'None':
+            end_col = begin_col
+        begin_col = int(begin_col)
+        end_col = int(end_col)
+        """Make sure that begin_col <= end_col (switch if not)"""
+        if begin_col > end_col:
+            tmp_col = end_col
+            end_col = begin_col
+            begin_col = tmp_col
+    else:
+        begin_col = end_col = ''
+
+    try:
+        fo = open(out_file,'w')
+    except:
+        print >> sys.stderr, "Unable to open output file"
+        sys.exit()
+
+    """
+    len1 is the number of lines in inp1_file
+    lines1 is the set of unique lines in inp1_file
+    diff1 is the number of duplicate lines removed from inp1_file
+    """
+    len1, lines1 = get_lines(inp1_file, begin_col, end_col, options.ignore_empty_end_cols)
+    diff1 = len1 - len(lines1)
+    len2, lines2 = get_lines(inp2_file, begin_col, end_col, options.ignore_empty_end_cols)
+    
+    lines1.difference_update(lines2)
+    """lines1 is now the set of unique lines in inp1_file - the set of unique lines in inp2_file"""
+
+    for line in lines1:
+        print >> fo, line
+
+    fo.close()
+    
+    info_msg = 'Subtracted %d lines. ' %((len1 - diff1) - len(lines1))
+    
+    if begin_col and end_col:
+        info_msg += 'Restricted to columns c' + str(begin_col) + ' thru c' + str(end_col) + '. '
+
+    if diff1 > 0:
+        info_msg += 'Eliminated %d duplicate/blank/comment/invalid lines from first query.' %diff1
+    
+    print info_msg
+
+if __name__ == "__main__":
+    main()
--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/subtract_query.xml	Mon Jul 27 08:14:06 2015 -0400
@@ -0,0 +1,126 @@
+<tool id="subtract_query1" name="Subtract Whole Dataset" version="0.1">
+  <description>from another dataset</description>
+  <command interpreter="python">
+    subtract_query.py $input1 $input2 $begin_col $end_col $output
+    #if str($ignore_empty_end_cols) == 'true':
+      --ignore-empty-end-cols
+    #end if
+
+  </command>
+  <inputs>
+    <param format="txt" name="input2" type="data" label="Subtract" help="Second dataset" />
+    <param format="txt" name="input1" type="data" label="from" help="First dataset" />
+    <param name="begin_col" type="data_column" data_ref="input1" force_select="False" label="Restrict subtraction between 'begin column'" />
+    <param name="end_col" type="data_column" data_ref="input1" force_select="False" label="and 'end column'" help="Specifying columns for restricting subtraction is available only for tabular formatted datasets" />
+    <param name="ignore_empty_end_cols" type="boolean" label="Ignore empty columns and whitespace at end of line when subtracting"/>
+  </inputs>
+  <outputs>
+    <data format="input" name="output" metadata_source="input1" />
+  </outputs>
+  <tests>
+  	<!-- Subtract 2 non-tabular files with no column restrictions. -->
+  	<!-- Cannot figure out why this test won't pass, it works in real time...
+    <test>
+      <param name="input1" value="1.txt" />
+      <param name="input2" value="2.txt" />
+      <param name="begin_col" value="None" />
+      <param name="end_col" value="None" />
+      <output name="output" file="subtract-query-1.dat" />
+    </test>
+    -->
+  	<!-- Subtract 2 tabular files with no column restrictions. -->
+    <test>
+      <param name="input1" value="eq-showbeginning.dat" />
+      <param name="input2" value="eq-showtail.dat" />
+      <param name="begin_col" value="None" />
+      <param name="end_col" value="None" />
+      <output name="output" file="subtract-query-2.dat" />
+    </test>
+  	<!-- Subtract 2 tabular files with column restrictions. -->
+    <test>
+      <param name="input1" value="eq-showbeginning.dat" />
+      <param name="input2" value="eq-removebeginning.dat" />
+      <param name="begin_col" value="c1" />
+      <param name="end_col" value="c3" />
+      <output name="output" file="subtract-query-3.dat" />
+    </test>
+  	<!-- Subtract a non-tabular file from a tabular file with no column restrictions. -->
+    <test>
+      <param name="input1" value="eq-showbeginning.dat" />
+      <param name="input2" value="2.txt" />
+      <param name="begin_col" value="None" />
+      <param name="end_col" value="None" />
+      <output name="output" file="subtract-query-4.dat" />
+    </test>
+    <!-- Subtract  2 tabular files with no column restrictions, ignoring empty end columns. -->
+    <test>
+      <param name="input1" value="eq-showbeginning_e.dat" />
+      <param name="input2" value="eq-showtail.dat" />
+      <param name="begin_col" value="None" />
+      <param name="end_col" value="None" />
+      <param name="ignore_empty_end_cols" value="true" />
+      <output name="output" file="subtract-query-2.dat" />
+    </test>
+  </tests>
+  <help>
+
+.. class:: infomark
+
+**TIP:** This tool complements the tool in the **Operate on Genomic Intervals** tool set which subtracts the intervals of two datasets.
+
+
+-----
+
+**Syntax**
+
+This tool subtracts an entire dataset from another dataset.  
+
+- Any text format is valid.
+- If both dataset formats are tabular, you may restrict the subtraction to specific columns **contained in both datasets** and the resulting dataset will include only the columns specified. 
+- The begin column must be less than or equal to the end column.  If it is not, begin column is switched with end column.
+- If begin column is specified but end column is not, end column will default to begin_column (and vice versa).
+- All blank and comment lines are skipped and not included in the resulting dataset (comment lines are lines beginning with a # character).
+- Duplicate lines are eliminated from both dataset prior to subtraction.  If any duplicate lines were eliminated from the first dataset, the number is displayed in the resulting history item.
+
+-----
+
+**Example**
+
+If this is the **First dataset**::
+
+  chr1            4225    19670
+  chr10           6       8
+  chr1            24417   24420
+  chr6_hla_hap2   0       150
+  chr2            1       5
+  chr10           2       10
+  chr1            30      55
+  chrY            1       20
+  chr1            1225979 42287290
+  chr10           7       8 
+
+and this is the **Second dataset**::
+
+  chr1            4225    19670
+  chr10           6       8
+  chr1            24417   24420
+  chr6_hla_hap2   0       150
+  chr2            1       5
+  chr1            30      55
+  chrY            1       20
+  chr1            1225979 42287290
+
+Subtracting the **Second dataset** from the **First dataset** (including all columns) will yield::
+
+  chr10           7       8 
+  chr10           2       10
+
+Conversely, subtracting the **First dataset** from the **Second dataset** (including all columns) will result in an empty dataset.
+
+Subtracting the **Second dataset** from the **First dataset** (restricting to columns c1 and c2) will yield::
+
+  chr10           7
+  chr10           2
+
+  </help>
+</tool>
\ No newline at end of file