# HG changeset patch # User serranop # Date 1379091885 14400 # Node ID fff15877fea719fa01e27a456515996f6090830d # Parent 7005f3eb8b68a51ca9e91f11328d13de401c78b5 Uploaded tool XML and test data diff -r 7005f3eb8b68 -r fff15877fea7 INSTALL diff -r 7005f3eb8b68 -r fff15877fea7 README diff -r 7005f3eb8b68 -r fff15877fea7 test-data/fastq_mergepairs_input1.fq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastq_mergepairs_input1.fq Fri Sep 13 13:04:45 2013 -0400 @@ -0,0 +1,12 @@ +@IRIS:7:1:29:952#0/1 +TGAGAAGCAAGAAGAAGGTTGGTTAGTGTTTTGGAG ++IRIS:7:1:29:952#0/1 +aaabaaaaaaaaaaa`aaY`aa^aaa^a_a_`aa`` +@IRIS:7:14:818:1331#0/1 +TTGGTTAGTGTTTTGGAGTCAAATATGACTTGATGT ++IRIS:7:14:818:1331#0/1 +aabb^a`a\`^aaa`_]_]]`aa`a_`\[__[]`[[ +@IRIS:7:1:17:394#0/1 +GTCAGGACAAGAAAGACAANTCCAATTNACATTATG ++IRIS:7:1:17:394#0/1 +aaabaa`]baaaaa_aab]D^^`b`aYDW]abaa`^ diff -r 7005f3eb8b68 -r fff15877fea7 test-data/fastq_mergepairs_input2.fq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastq_mergepairs_input2.fq Fri Sep 13 13:04:45 2013 -0400 @@ -0,0 +1,12 @@ +@IRIS:7:1:29:952#0/2 +GACTCCAAAACACTAACCAACCTTCTTCTTGCTTCT ++IRIS:7:1:29:952#0/2 +aaaabaaaabaaaabbaaaa````__`__^__``__ +@IRIS:7:14:818:1331#0/2 +CGTACACATGACATCAAGTCATATTTGACTCCAAAA ++IRIS:7:14:818:1331#0/2 +abababababbabaabba_`aabaaaa`````aaaa +@IRIS:7:1:17:394#0/2 +NTTTTCTATTAATACTCNCGNTTTGNGNNANAATCC ++IRIS:7:1:17:394#0/2 +BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBB diff -r 7005f3eb8b68 -r fff15877fea7 test-data/fastq_mergepairs_output.fq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/fastq_mergepairs_output.fq Fri Sep 13 13:04:45 2013 -0400 @@ -0,0 +1,8 @@ +@IRIS:7:1:29:952#0/1 +TGAGAAGCAAGAAGAAGGTTGGTTAGTGTTTTGGAGTC ++ +aaJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJaa +@IRIS:7:14:818:1331#0/1 +TTGGTTAGTGTTTTGGAGTCAAATATGACTTGATGTCATGTGTACG ++ +aabb^a`a\`JJJJJJJJJJJJJJJJJJJJJJJJJJbababababa diff -r 7005f3eb8b68 -r fff15877fea7 usearch_fastq_mergepairs.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/usearch_fastq_mergepairs.xml Fri Sep 13 13:04:45 2013 -0400 @@ -0,0 +1,81 @@ + + merging of paired reads + usearch -version + usearch + -fastq_mergepairs '$input_forward' + -reverse '$input_reverse' + -fastq_qmaxout $qmaxout + -fastqout '$output' + + + + + + + + + + + + + + + + + + +**What it Does** + +Performs merging of paired reads. + +The FASTQ filename for the forward reads is specified by the -fastq_mergepairs option, and the reverse read filename is specified by the -reverse option. Output files are specified by -fastqout (for FASTQ) and / or -fastaout (for FASTA). + +Forward and reverse must be in 1:1 correspondence and must appear in the same order in both files. The labels for the forward and reverse read in a given pair must be identical except for a single position where a '1' appears in the forward read label and a '2' appears in the reverse read label. + +----- + +**Input formats** + +Forward read:: + + @IRIS:7:1:29:952#0/1 + TGAGAAGCAAGAAGAAGGTTGGTTAGTGTTTTGGAG + +IRIS:7:1:29:952#0/1 + aaabaaaaaaaaaaa`aaY`aa^aaa^a_a_`aa`` + +Reverse read:: + + @IRIS:7:1:29:952#0/2 + GACTCCAAAACACTAACCAACCTTCTTCTTGCTTCT + +IRIS:7:1:29:952#0/2 + aaaabaaaabaaaabbaaaa````__`__^__``__ + +----- + +**Output** + +A multiple-fastq file, for example:: + + @IRIS:7:1:29:952#0/1 + TGAGAAGCAAGAAGAAGGTTGGTTAGTGTTTTGGAGTC + + + aaJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJaa + +------ + +**Author** + +Robert C. Edgar (robert@drive5.com) + +**Manual** + +http://drive5.com/usearch/manual/fastq_mergepairs.html + +**Citation** + +Please cite one of these papers if you use USEARCH in published work. + +Edgar,RC (2010) Search and clustering orders of magnitude faster than BLAST, Bioinformatics 26(19), 2460-2461. +doi: 10.1093/bioinformatics/btq461 + +