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annotate qualimap_bamqc.py @ 7:34310c8c59f9 draft

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planemo upload for repository https://github.com/scottx611x/qualimap2 commit 070be0ead8afa05bfbb3075f075a97e7c32d619e-dirty
author scottx611x
date Thu, 26 Jul 2018 11:34:21 -0400
parents 5b36882f6455
children 4e5e0a116434
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716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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1 #!/usr/bin/env python
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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2 from __future__ import print_function
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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3 import argparse
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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4 from subprocess import check_call, CalledProcessError
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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5 import sys
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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6 import logging
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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8 log = logging.getLogger(__name__)
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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11 def qualimap_bamqc(bam_filename, genomecov_file, out_dir, jv_mem_size):
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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12 qualimap_command = [
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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13 "qualimap", "bamqc",
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14 "-bam " + bam_filename,
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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15 "-oc " + genomecov_file,
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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16 "-outdir " + out_dir,
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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17 "--java-mem-size=" + jv_mem_size
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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18 ]
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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20 try:
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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21 check_call(qualimap_command)
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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22 except CalledProcessError:
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23 print("Error running the qualimap bamqc", file=sys.stderr)
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26 def main():
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27 parser = argparse.ArgumentParser(
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28 description="Generate Bam Quality Statistics"
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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29 )
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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30 parser.add_argument('--input_file')
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31 parser.add_argument('--out_genome_file', default="genome_coverage.txt")
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32 parser.add_argument('--out_dir')
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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33 parser.add_argument('--java_mem_size', default="8G")
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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35 args = parser.parse_args()
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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37 qualimap_bamqc(
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38 args.input_file,
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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39 args.out_genome_file,
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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40 args.out_dir,
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41 args.java_mem_size
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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42 )
716e406ce6ea planemo upload for repository https://github.com/scottx611x/qualimap2 commit 2e9620ea29d3a146e8669ec0037932d9a2135c79-dirty
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45 if __name__ == "__main__":
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46 main()