Mercurial > repos > sblanck > smat
diff GEOQuery.xml @ 0:f32198f08f27 draft default tip
Imported from capsule None
author | sblanck |
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date | Fri, 09 Sep 2016 04:23:04 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/GEOQuery.xml Fri Sep 09 04:23:04 2016 -0400 @@ -0,0 +1,62 @@ +<tool id="GEOQuery" name="GEOQuery"> + <description>GEOQuery wrapper</description> + <requirements> + <container type="docker">sblanck/smat</container> + </requirements> + <command> + /galaxy-tools/stderr_wrapper.py Rscript + /galaxy-tools/transcriptomics/GEOquery/GEOQuery.R + $GEOQueryID + $GEOQueryData + $GEOQueryRData + $conditions + $transformation + </command> + + <inputs> + <param name="GEOQueryID" type="text" size="12" optional="false" label="GEOQuery ID" help=""> + <validator type="empty_field"/> + </param> + <param name="transformation" type="select" label="log2 transformation"> + <option value="auto">auto</option> + <option value="yes">yes</option> + <option value="no">no</option> + </param> + </inputs> + + <outputs> + <data format="tabular" name="GEOQueryData" label="GEOQuery Data of ${GEOQueryID}"/> + <data format="cond" name="conditions" label="conditions of ${GEOQueryID}"/> + <data format="rdata" name="GEOQueryRData" label="GEOQuery RData of ${GEOQueryID}"/> + </outputs> +<help> +**What it does** + +This tool fetches microarray data directly from GEO database, based on the GEOQuery R package. Given a GSE accession ID, it returns an Rdata object containing the data and a text file (.cond file) summarizing the conditions of the experiment. +The .cond file is a text file containing one line per sample in the experiment. Each line is made of 3 columns: + + - Sample ID + - Condition of the biological sample + - Description of the biological sample + +**Example** of .cond file +:: + + Sample ID Condition Description + GSM80460 series of 16 tumors GSM80460 OSCE-2T SERIES OF 16 TUMORS + GSM80461 series of 16 tumors GSM80461 OSCE-4T Series of 16 Tumors + GSM80462 series of 16 tumors GSM80462 OSCE-6T Series of 16 Tumors + GSM80476 series of 4 normals GSM80476 OSCE-2N Series of 4 Normals + GSM80477 series of 4 normals GSM80477 OSCE-9N Series of 4 Normals + +**Results** + +- Rdata object containing data for further analysis +- Condition (.cond) file summarizing conditions of the experiment +- Tabular (.txt) file containing expression data for each sample + + +</help> + + +</tool>