# HG changeset patch # User sblanck # Date 1529914282 14400 # Node ID 25b828010ca9f80a42f866acf506913f787edbd2 # Parent 22e1325aaa88b41a463555f24de5e8acc1b533f4 planemo upload for repository https://github.com/sblanck/smagexp/tree/master/smagexp_tools commit bfd179bd7fb4796e21183e0e4de2fcf78ca35b7d diff -r 22e1325aaa88 -r 25b828010ca9 ImportDataFromMatrix.R --- a/ImportDataFromMatrix.R Fri Jun 22 04:30:31 2018 -0400 +++ b/ImportDataFromMatrix.R Mon Jun 25 04:11:22 2018 -0400 @@ -66,13 +66,10 @@ colnames(conditions)=c("source_name_ch1","description") phenodata<-new("AnnotatedDataFrame",data=conditions) -head(data) -conditions - eset=ExpressionSet(assayData=data,phenoData=phenodata,annotation=annotation) if (normalization == "quantile") { - eset <- normalize.ExpressionSet.quantiles(eset, transfn="log2") + eset <- normalize.ExpressionSet.quantiles(eset, transfn="log") } else if (normalization == "log2") { exprs(eset) = log2(exprs(eset)) } diff -r 22e1325aaa88 -r 25b828010ca9 MetaRNAseq.xml --- a/MetaRNAseq.xml Fri Jun 22 04:30:31 2018 -0400 +++ b/MetaRNAseq.xml Mon Jun 25 04:11:22 2018 -0400 @@ -70,10 +70,12 @@ Given several DESeq2 results this tool runs a meta-analysis using the metaRNAseq R package. **Inputs** -At list 2 studies -- Results of DESeq2 study -- Number of replicate of the study +- At least 2 studies, and for each study + - Results of DESeq2 study + - Number of replicates of the study +- A FDR Threshold for a gene to be declared differentially expressed + **Results** - Venn Diagram or upsetR diagram (when the number of studies is greater than 2) summarizing the results of the meta-analysis @@ -84,8 +86,8 @@ - Loss : Number of genes that are identified differentially expressed in single studies but not in meta-analysis - DR (Integration-driven Discovery Rate) : corresponding proportion of IDD - IRR (Integration-driven Revision) : corresponding proportion of Loss - -- Fully sortable and requestable table, with gene annotations and hypertext links to NCBI gene database. + +It also generates a text file containing summarization of the results of each single analysis and meta-analysis. Potential conflicts between single analyses are indicated by zero values in the "signFC" column. ]]> diff -r 22e1325aaa88 -r 25b828010ca9 Recount.xml --- a/Recount.xml Fri Jun 22 04:30:31 2018 -0400 +++ b/Recount.xml Mon Jun 25 04:11:22 2018 -0400 @@ -44,7 +44,7 @@ **Results** -- Tabular file containing meta-data +- Tabular file containing samples ID and samples conditions - One count file per sample ]]>