diff breakMAF.pl @ 1:0554fa091710 draft

planemo upload for repository https://github.com/bgruening/galaxytools/tree/master/tools/rna_tools/rnacode commit b6d3800e260cdfcbe99e5f056344c3df101dad00

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/breakMAF.pl	Fri Apr 13 07:37:15 2018 -0400
@@ -0,0 +1,312 @@
+#!/usr/bin/perl -w
+
+use strict;
+use Getopt::Long;
+use POSIX qw(ceil floor);
+
+my $maxLength     = 400;
+my $desiredLength = 200;
+my $help          = 0;
+
+GetOptions(
+  "maxLength:i"     => \$maxLength,
+  "desiredLength:i" => \$desiredLength,
+  "help"            => \$help,
+  "h"               => \$help
+);
+
+if ($help) {
+  print STDERR "\nbreakMAF [options] < input.maf > output.maf\n\n";
+  print STDERR "Options:\n";
+  print STDERR "  maxLength:     Break all blocks longer than that (default: 400 columns)\n";
+  print STDERR "  desiredLength: Try to create blocks of this size (default: 200 columns)\n";
+  exit(1);
+}
+
+$/ = 'a score=';
+
+while ( my $block = <> ) {
+
+  $block = 'a score=' . $block;
+
+  my $currAln = readMAF($block)->[0];
+
+  next if not defined($currAln);
+
+  my $length = length( $currAln->[0]->{seq} );
+
+  if ( $length > $maxLength ) {
+
+    my $breakN      = int( $length / $desiredLength );
+    my $chunkLength = ceil( $length / $breakN );
+
+    my $from = 0;
+
+    while (1) {
+      my $to = $from + $chunkLength;
+
+      if ( $to > $length ) {
+        $to = $length;
+      }
+
+      my $slice = sliceAlnByColumn( $currAln, $from, $to );
+
+      print formatAln( $slice, 'maf' ), "\n";
+
+      $from = $to;
+
+      last if $from == $length;
+    }
+
+  } else {
+    print formatAln( $currAln, 'maf' ), "\n";
+  }
+}
+
+######################################################################
+#
+# readMAF($string)
+#
+# Converts the MAF in string to internal alignment format. Returns
+# list of usual array references.
+#
+######################################################################
+
+sub readMAF {
+
+  my $string = shift;
+
+  return [] if $string eq '';
+
+  my @input = split( "\n", $string );
+
+  #open(FILE,"<$file") || die("Could not read $file ($!)");
+
+  my @outAlns = ();
+  my @aln     = ();
+
+  foreach my $i ( 0 .. $#input ) {
+
+    $_ = $input[$i];
+
+    next if (/^\s?\#/);
+    next if (/^\s?a/);
+
+    if (/^\s?s/) {
+      ( my $dummy, my $name, my $start, my $length, my $strand, my $fullLength, my $seq ) = split;
+
+      my $end = $start + $length;
+
+      ( my $org, my $chrom ) = split( /\./, $name );
+
+      push @aln, {
+        name   => $name,
+        org    => $org,
+        chrom  => $chrom,
+        start  => $start,
+        end    => $end,
+        seq    => $seq,
+        strand => $strand
+        };
+    }
+
+    if ( /^\s?$/ and @aln ) {
+      push @outAlns, [@aln];
+      @aln = ();
+    }
+    if ( ( not defined $input[ $i + 1 ] ) and (@aln) ) {
+      push @outAlns, [@aln];
+      @aln = ();
+    }
+  }
+  return \@outAlns;
+}
+
+sub formatAln {
+
+  my @aln = @{ $_[0] };
+
+  my $format = $_[1];
+
+  $format = lc($format);
+
+  my @alnSeqs  = ();
+  my @alnNames = ();
+
+  my $counter = 1;
+
+  foreach my $row (@aln) {
+
+    my $name = "seq$counter";
+    $counter++;
+    if ( $row->{name} ) {
+      $name = ( $row->{name} );
+    } elsif ( $row->{org} and $row->{chrom} ) {
+      $name = "$row->{org}.$row->{chrom}";
+    }
+
+    my $start  = $row->{start};
+    my $end    = $row->{end};
+    my $strand = $row->{strand};
+
+    my $pos = '';
+
+    if (  defined $start
+      and defined $end
+      and defined $strand
+      and ( $format ne 'phylip' ) ) {
+      ( $start, $end ) = ( $end, $start ) if ( $strand eq '-' );
+      $pos = "/$start-$end";
+    }
+
+    push @alnNames, "$name$pos";
+    push @alnSeqs,  $row->{seq};
+
+  }
+
+  my $output = '';
+
+  if ( $format eq 'clustal' ) {
+
+    $output = "CLUSTAL W(1.81) multiple sequence alignment\n\n\n";
+    my $maxName = 0;
+
+    foreach my $name (@alnNames) {
+      $maxName = ( $maxName < length($name) ) ? length($name) : $maxName;
+    }
+
+    for my $i ( 0 .. $#alnNames ) {
+      my $buffer = " " x ( ( $maxName + 6 ) - length( $alnNames[$i] ) );
+      $alnNames[$i] .= $buffer;
+    }
+    my $columnWidth = 60;
+    my $currPos     = 0;
+    my $length      = length( $alnSeqs[0] );
+
+    while ( $currPos < $length ) {
+      for my $i ( 0 .. $#alnNames ) {
+        $output .= $alnNames[$i];
+        $output .= substr( $alnSeqs[$i], $currPos, $columnWidth );
+        $output .= "\n";
+      }
+      $output .= "\n\n";
+      $currPos += $columnWidth;
+    }
+  } elsif ( $format eq 'fasta' ) {
+    foreach my $i ( 0 .. $#alnNames ) {
+      my $name = $alnNames[$i];
+      my $seq  = $alnSeqs[$i];
+      $seq =~ s/(.{60})/$1\n/g;
+      $output .= ">$name\n$seq\n";
+    }
+  } elsif ( $format eq 'phylip' ) {
+    my $length = length( $alnSeqs[0] );
+    my $N      = @alnSeqs;
+    $output .= "  $N $length\n";
+    foreach my $i ( 0 .. $#alnNames ) {
+      my $name = $alnNames[$i];
+      my $seq  = $alnSeqs[$i];
+      $seq =~ s/(.{60})/$1\n/g;
+      $output .= "$name\n$seq\n";
+    }
+  } elsif ( $format eq 'maf' ) {
+    $output .= "a score=0\n";
+    foreach my $row (@aln) {
+      my $length = $row->{end} - $row->{start};
+      $output .= "s $row->{org}.$row->{chrom} $row->{start} $length $row->{strand} 0 $row->{seq}\n";
+    }
+  }
+  return $output;
+
+}
+
+######################################################################
+#
+# sliceAlnByColumn(\@aln ref-to-alignment, $start int, $end int)
+#
+# Returns slice of alignment specified by alignment column.
+#
+# \@aln ... alignment in list of hash format
+# $start, $end ... slice to cut
+#
+# Returns reference to alignment in list of hash format. This is a new
+# alignment, i.e. the input is not sliced in place
+#
+######################################################################
+
+sub sliceAlnByColumn {
+
+  my @aln = @{ $_[0] };
+  shift;
+  ( my $start, my $end ) = @_;
+
+  # correct ends without warning if outside of valid range
+  $start = 0 if ( $start < 0 );
+  $end = length( $aln[0]->{seq} ) if ( $end > length( $aln[0]->{seq} ) );
+
+  #my @newAln=@aln;
+
+  # make deep copy of list of hash
+  my @newAln = ();
+  foreach (@aln) {
+    push @newAln, { %{$_} };
+  }
+
+  foreach my $i ( 0 .. $#newAln ) {
+
+    my $oldStart = $newAln[$i]->{start};
+    my $oldEnd   = $newAln[$i]->{end};
+
+    $newAln[$i]->{start} = alnCol2genomePos( $newAln[$i]->{seq}, $oldStart, $start );
+    $newAln[$i]->{end}   = alnCol2genomePos( $newAln[$i]->{seq}, $oldStart, $end - 1 ) + 1;
+    $newAln[$i]->{seq} = substr( $newAln[$i]->{seq}, $start, $end - $start );
+
+  }
+
+  return ( [@newAln] );
+
+}
+
+######################################################################
+#
+# alnCol2genomePos($seq string, $start int, $col int)
+#
+# Calculates the genomic position corresponding to a column in an
+# alignment.
+#
+# $seq ... sequence from alignment (i.e. letters with gaps)
+# $start ... Genomic position of first letter in $seq
+# $col ... column in the alignment that is to be mapped
+#
+# Returns genomic position. No error handling, so $col must be a valid
+# column of the string $seq.
+#
+#######################################################################
+
+sub alnCol2genomePos {
+
+  ( my $seq, my $start, my $col ) = @_;
+
+  $seq =~ s/\./-/g;    #Convert all gaps to "-"
+
+  my $newPos = $start;
+
+  # if gap only...
+  return $start if ( $seq =~ /^-+$/ );
+
+  ( my $tmp ) = $seq =~ /(-*)[^-]/;
+
+  my $leadingGaps = length($tmp);
+
+  # if column is in gap before first letter,
+  # return position of the first letter
+  return $start if ( $col < $leadingGaps );
+
+  $newPos = $start - 1;
+
+  for my $i ( $leadingGaps .. $col ) {
+    $newPos++ if ( ( my $t = substr( $seq, $i, 1 ) ) ne '-' );
+  }
+  return $newPos;
+}
+