Mercurial > repos > rnateam > graphclust_preprocessing
diff preprocessing.xml @ 0:a0f4b85dc739 draft
planemo upload for repository https://github.com/eteriSokhoyan/galaxytools/tree/branchForIterations/tools/GraphClust commit 21aaee40723b5341b4236edeb0e72995c2054053
author | rnateam |
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date | Fri, 16 Dec 2016 07:33:19 -0500 |
parents | |
children | 35d2fb6f3647 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/preprocessing.xml Fri Dec 16 07:33:19 2016 -0500 @@ -0,0 +1,85 @@ +<tool id="preproc" name="Preprocessing" version="0.1"> + <requirements> + <requirement type="package" version="0.1">graphclust-wrappers</requirement> + </requirements> + <stdio> + <exit_code range="1:" /> + </stdio> + <command> + <![CDATA[ + + 'preprocessing.pl' + '$fastaFile' $max_length $in_winShift $min_seq_length + +]]> + </command> + <inputs> + <param type="data" name="fastaFile" format="fasta" /> + <param name="max_length" type="integer" value="10000" size="5" label="window size"/> + <param name="in_winShift" type="integer" value="100" size="5" label="window shift in percent"/> + <param name="min_seq_length" type="integer" value="5" size="5" label="minimum sequence length"/> + </inputs> + + <outputs> + <data name="data.fasta" format="fasta" from_work_dir="FASTA/data.fasta" label="data.fasta"/> + <data name="data.map" format="txt" from_work_dir="FASTA/data.map" label="data.map"/> + <data name="data.names" format="txt" from_work_dir="FASTA/data.names" label="data.names"/> + <data name="data.fasta.scan" format="fasta" from_work_dir="FASTA/data.fasta.scan" label="data.fasta.scan"/> + <data name="FASTA" format="zip" from_work_dir="FASTA.zip" label="FASTA.ZIP"/> + </outputs> + + + <tests> + <test> + <param name="fastaFile" value="input.fa"/> + <param name="max_length" value="10000"/> + <param name="in_winShift" value="100"/> + <param name="min_seq_length" value="5"/> + <output name="data.fasta" file="FASTA/data.fasta"/> + <output name="data.map" file="FASTA/data.map" /> + <output name="data.names" file="FASTA/data.names"/> + <output name="data.fasta.scan" file="FASTA/data.fasta.scan" /> + </test> +</tests> + + <help> +<![CDATA[ + +**What it does** + +The tool takes as an input file of sequences in Fasta format and creates the final input for GraphCLust based on given parameters. + +**Parameters** + ++ **window size** : All input sequences are splitted into fragments of this length. + The shift of the sliding window can be defined via option *window shift in percent*. + This paramter reflects the expected length of signals to be found. + Slightly larger windows are usually ok. Too small windows can disturb existing signals. + + + + ++ **window shift in percent** : Relative window size in % for window shift during input preprocessing. + Please note that a small shift results in much more fragments for clustering. The benefit is that RNA + motifs/structures are not destroyed by arbitrary split points. Smaller + shifts usually increase the cluster quality. Too small shifts (<20) are not + recommended as a dense center is "polluted" by overlapping fragments and + no other occurences in the dataset can be found. + + + + + ++ **minimum sequence length** : Minimal length of input sequences. + Every input sequence below that length is ignored completely during clustering. + + + ]]></help> + + + <citations> + <citation type="doi">10.1093/bioinformatics/bts224</citation> + </citations> + + +</tool>