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view prepare_population_structure.py @ 0:3be071295340 default tip
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author | rico |
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date | Fri, 06 Apr 2012 15:50:15 -0400 |
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#!/usr/bin/env python import errno import os import shutil import subprocess import sys from Population import Population import gd_composite ################################################################################ def do_import(filename, files_path, min_reads, min_qual, min_spacing, tags, using_info, population_list): info_page = gd_composite.InfoPage() info_page.set_title('Prepare to look for population structure Galaxy Composite Dataset') display_file = gd_composite.DisplayFile() display_value = gd_composite.DisplayValue() out_ped = gd_composite.Parameter(name='admix.ped', value='admix.ped', display_type=display_file) out_map = gd_composite.Parameter(name='admix.map', value='admix.map', display_type=display_file) out_use = gd_composite.Parameter(description=using_info, display_type=display_value) info_page.add_output_parameter(out_ped) info_page.add_output_parameter(out_map) info_page.add_output_parameter(out_use) in_min_reads = gd_composite.Parameter(description='Minimum reads covering a SNP, per individual', value=min_reads, display_type=display_value) in_min_qual = gd_composite.Parameter(description='Minimum quality value, per individual', value=min_qual, display_type=display_value) in_min_spacing = gd_composite.Parameter(description='Minimum spacing between SNPs on the same scaffold', value=min_spacing, display_type=display_value) info_page.add_input_parameter(in_min_reads) info_page.add_input_parameter(in_min_qual) info_page.add_input_parameter(in_min_spacing) misc_populations = gd_composite.Parameter(name='Populations', value=population_list, display_type=gd_composite.DisplayPopulationList()) info_page.add_misc(misc_populations) with open(filename, 'w') as ofh: print >> ofh, info_page.render() def mkdir_p(path): try: os.makedirs(path) except OSError, e: if e.errno <> errno.EEXIST: raise def die(message, exit=True): print >> sys.stderr, message if exit: sys.exit(1) ################################################################################ if len(sys.argv) < 9: die("Usage") # parse command line input_snp_filename, min_reads, min_qual, min_spacing, output_filename, output_files_path = sys.argv[1:7] args = sys.argv[7:] individual_metadata = [] population_files = [] population_names = [] all_individuals = False for arg in args: if arg == 'all_individuals': all_individuals = True elif len(arg) > 11: tag = arg[:11] value = arg[11:] if tag == 'individual:': individual_metadata.append(value) elif tag == 'population:': filename, name = value.split(':', 1) population_files.append(filename) population_names.append(name) p_total = Population() p_total.from_tag_list(individual_metadata) individual_population = {} population_list = [] if all_individuals: p1 = p_total p1.name = 'All Individuals' population_list.append(p1) else: p1 = Population() for idx in range(len(population_files)): population_file = population_files[idx] population_name = population_names[idx] this_pop = Population(population_name) this_pop.from_population_file(population_file) population_list.append(this_pop) p1.from_population_file(population_file) tags = p1.tag_list() for tag in tags: if tag not in individual_population: individual_population[tag] = population_name if not p_total.is_superset(p1): print >> sys.stderr, 'There is an individual in the population that is not in the SNP table' sys.exit(1) # run tool prog = 'admix_prep' args = [] args.append(prog) args.append(input_snp_filename) args.append(min_reads) args.append(min_qual) args.append(min_spacing) tags = p1.tag_list() for tag in tags: args.append(tag) #print "args:", ' '.join(args) p = subprocess.Popen(args, bufsize=-1, stdin=None, stdout=subprocess.PIPE, stderr=sys.stderr) (stdoutdata, stderrdata) = p.communicate() rc = p.returncode if rc != 0: die('admix_prep failed: rc={0}'.format(rc)) using_info = stdoutdata.rstrip('\r\n') mkdir_p(output_files_path) output_ped_filename = os.path.join(output_files_path, 'admix.ped') output_map_filename = os.path.join(output_files_path, 'admix.map') shutil.copy2('admix.ped', output_ped_filename) shutil.copy2('admix.map', output_map_filename) do_import(output_filename, output_files_path, min_reads, min_qual, min_spacing, tags, using_info, population_list) os.unlink('admix.ped') os.unlink('admix.map') sys.exit(0)