Mercurial > repos > peterjc > tmhmm_and_signalp
annotate tools/protein_analysis/psortb.xml @ 18:2b35b5c4b7f4 draft
Uploaded v0.2.5, preview 2, fixed bug in RXLR tools
| author | peterjc |
|---|---|
| date | Fri, 10 May 2013 07:48:26 -0400 |
| parents | af3174637834 |
| children | 4cd848c5590b |
| rev | line source |
|---|---|
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af3174637834
Uploaded v0.2.5 preview 1, adding links to Tool Shed entry
peterjc
parents:
11
diff
changeset
|
1 <tool id="Psortb" name="psortb" version="0.0.2"> |
| 8 | 2 <description>Determines sub-cellular localisation of bacterial/archaeal protein sequences</description> |
| 3 <!-- If job splitting is enabled, break up the query file into parts --> | |
| 4 <!-- Using 2000 chunks meaning 4 threads doing 500 each is ideal --> | |
| 5 <parallelism method="basic" split_inputs="fasta_file" split_mode="to_size" split_size="2000" merge_outputs="tabular_file"></parallelism> | |
| 6 <version_command interpreter="python">psortb.py --version</version_command> | |
| 7 <command interpreter="python">psortb.py "\$NSLOTS" "$type" "$long" "$cutoff" "$divergent" "$sequence" "$outfile"</command> | |
| 8 <stdio> | |
| 9 <!-- Anything other than zero is an error --> | |
| 10 <exit_code range="1:" /> | |
| 11 <exit_code range=":-1" /> | |
| 12 </stdio> | |
| 13 <inputs> | |
| 14 <param format="fasta" name="sequence" type="data" | |
| 15 label="Input sequences for which to predict localisation (protein FASTA format)" /> | |
| 16 <param name="type" type="select" | |
| 17 label="Organism type (N.B. all sequences in the above file must be of the same type)" > | |
| 18 <option value="-p">Gram positive bacteria</option> | |
| 19 <option value="-n">Gram negative bacteria</option> | |
| 20 <option value="-a">Archaea</option> | |
| 21 </param> | |
| 22 <param name="long" type="select" label="Output type"> | |
| 23 <option value="terse">Short (terse, tabular with 3 columns)</option> | |
| 24 <!-- The normal output is text, not tabular - worth offering? | |
| 25 <option value="normal">Normal</option> | |
| 26 --> | |
| 27 <option value="long">Long (verbose, tabular with about 30 columns, depending on organism type)</option> | |
| 28 </param> | |
| 29 <param name="cutoff" size="10" type="float" optional="true" value="" | |
| 30 label="Sets a cutoff value for reported results (e.g. 7.5)" | |
| 31 help="Leave blank or use zero for no cutoff." /> | |
| 32 <param name="divergent" size="10" type="float" optional="true" value="" | |
| 33 label="Sets a cutoff value for the multiple localization flag (e.g. 4.5)" | |
| 34 help="Leave blank or use zero for no cutoff." /> | |
| 35 </inputs> | |
| 36 <outputs> | |
| 37 <data format="tabular" name="outfile" /> | |
| 38 </outputs> | |
| 39 <requirements> | |
| 40 <requirement type="binary">psort</requirement> | |
| 41 </requirements> | |
| 42 <tests> | |
| 43 <test> | |
| 11 | 44 <param name="sequence" value="empty.fasta" ftype="fasta"/> |
| 45 <param name="long" value="terse"/> | |
| 46 <output name="outfile" file="empty_psortb_terse.tabular" ftype="tabular"/> | |
| 47 </test> | |
| 48 <test> | |
| 8 | 49 <param name="sequence" value="k12_ten_proteins.fasta" ftype="fasta"/> |
| 50 <param name="long" value="terse"/> | |
| 51 <output name="outfile" file="k12_ten_proteins_psortb_p_terse.tabular" ftype="tabular"/> | |
| 52 </test> | |
| 53 </tests> | |
| 54 <help> | |
| 55 | |
| 56 **What it does** | |
| 57 | |
| 58 This calls the command line tool PSORTb v3.0 for prediction of prokaryotic | |
| 59 localization sites. The input dataset needs to be protein FASTA sequences. | |
| 60 The default output is a simple tabular file with three columns, one row | |
| 61 per query sequence: | |
| 62 | |
| 63 ====== ============================== | |
| 64 Column Description | |
| 65 ------ ------------------------------ | |
| 66 1 Sequence identifier | |
| 67 2 Localisation, e.g. Cytoplasmic | |
| 68 3 Score | |
| 69 ====== ============================== | |
| 70 | |
| 71 The long output is also tabular with one row per query sequence, but has | |
| 72 lots more columns (a different set for each supported organism type). In | |
| 73 both cases, a simple header line is included (starting with a hash, #, | |
| 74 so that Galaxy treats it as a comment) giving the column names. | |
| 75 | |
| 76 | |
| 77 **References** | |
| 78 | |
| 79 N.Y. Yu, J.R. Wagner, M.R. Laird, G. Melli, S. Rey, R. Lo, P. Dao, | |
| 80 S.C. Sahinalp, M. Ester, L.J. Foster, F.S.L. Brinkman (2010) | |
| 81 PSORTb 3.0: Improved protein subcellular localization prediction with | |
| 82 refined localization subcategories and predictive capabilities for all | |
| 83 prokaryotes, Bioinformatics 26(13):1608-1615 | |
| 84 http://dx.doi.org/10.1093/bioinformatics/btq249 | |
| 85 | |
| 86 http://www.psort.org/documentation/index.html | |
| 87 | |
|
17
af3174637834
Uploaded v0.2.5 preview 1, adding links to Tool Shed entry
peterjc
parents:
11
diff
changeset
|
88 This wrapper is available to install into other Galaxy Instances via the Galaxy |
|
af3174637834
Uploaded v0.2.5 preview 1, adding links to Tool Shed entry
peterjc
parents:
11
diff
changeset
|
89 Tool Shed at http://toolshed.g2.bx.psu.edu/view/peterjc/tmhmm_and_signalp |
| 8 | 90 </help> |
| 91 </tool> |