Mercurial > repos > peterjc > tmhmm_and_signalp
annotate tools/protein_analysis/promoter2.xml @ 18:2b35b5c4b7f4 draft
Uploaded v0.2.5, preview 2, fixed bug in RXLR tools
| author | peterjc |
|---|---|
| date | Fri, 10 May 2013 07:48:26 -0400 |
| parents | af3174637834 |
| children | 4cd848c5590b |
| rev | line source |
|---|---|
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af3174637834
Uploaded v0.2.5 preview 1, adding links to Tool Shed entry
peterjc
parents:
16
diff
changeset
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1 <tool id="promoter2" name="Promoter 2.0" version="0.0.5"> |
| 7 | 2 <description>Find eukaryotic PolII promoters in DNA sequences</description> |
| 3 <!-- If job splitting is enabled, break up the query file into parts --> | |
| 4 <!-- Using 2000 per chunk so 4 threads each doing 500 is ideal --> | |
| 5 <parallelism method="basic" split_inputs="fasta_file" split_mode="to_size" split_size="2000" merge_outputs="tabular_file"></parallelism> | |
| 6 <command interpreter="python"> | |
| 7 promoter2.py "\$NSLOTS" $fasta_file $tabular_file | |
| 8 ##Set the number of threads in the runner entry in universe_wsgi.ini | |
| 9 ##which (on SGE at least) will set the $NSLOTS environment variable. | |
| 10 ##If the environment variable isn't set, get "", and defaults to one. | |
| 11 </command> | |
| 12 <stdio> | |
| 13 <!-- Anything other than zero is an error --> | |
| 14 <exit_code range="1:" /> | |
| 15 <exit_code range=":-1" /> | |
| 16 </stdio> | |
| 17 <inputs> | |
| 18 <param name="fasta_file" type="data" format="fasta" label="FASTA file of DNA sequences"/> | |
| 19 </inputs> | |
| 20 <outputs> | |
| 21 <data name="tabular_file" format="tabular" label="Promoter2 on ${fasta_file.name}" /> | |
| 22 </outputs> | |
| 23 <requirements> | |
| 24 <requirement type="binary">promoter</requirement> | |
| 25 </requirements> | |
| 16 | 26 <tests> |
| 27 <test> | |
| 28 <param name="fasta_file" value="Adenovirus.fasta" ftype="fasta"/> | |
| 29 <output name="tabular_file" file="Adenovirus.promoter2.tabular" ftype="tabular"/> | |
| 30 </test> | |
| 31 <test> | |
| 32 <param name="fasta_file" value="empty.fasta" ftype="fasta"/> | |
| 33 <output name="tabular_file" file="empty_promoter2.tabular" ftype="tabular"/> | |
| 34 </test> | |
| 35 </tests> | |
| 7 | 36 <help> |
| 37 | |
| 38 **What it does** | |
| 39 | |
| 40 This calls the Promoter 2.0 tool for prediction of eukaryotic PolII promoter sequences using a Neural Network (NN) model. | |
| 41 | |
| 42 The input is a FASTA file of nucleotide sequences (e.g. upstream regions of your genes), and the output is tabular with five columns (one row per promoter): | |
| 43 | |
| 44 1. Sequence identifier (first word of FASTA header) | |
| 45 2. Promoter position, e.g. 600 | |
| 46 3. Promoter score, e.g. 1.063 | |
| 47 4. Promoter likelihood, e.g. Highly likely prediction | |
| 48 | |
| 49 The scores are classified very simply as follows: | |
| 50 | |
| 51 ========= ======================== | |
| 52 Score Description | |
| 53 --------- ------------------------ | |
| 54 below 0.5 ignored | |
| 55 0.5 - 0.8 Marginal prediction | |
| 56 0.8 - 1.0 Medium likely prediction | |
| 57 above 1.0 Highly likely prediction | |
| 58 ========= ======================== | |
| 59 | |
| 60 Internally the input FASTA file is divided into parts (to allow multiple processors to be used), and the raw output is reformatted into this tabular layout suitable for downstream analysis within Galaxy. | |
| 61 | |
| 62 **References** | |
| 63 | |
| 64 Knudsen. | |
| 65 Promoter2.0: for the recognition of PolII promoter sequences. | |
| 66 Bioinformatics, 15:356-61, 1999. | |
| 67 http://dx.doi.org/10.1093/bioinformatics/15.5.356 | |
| 68 | |
| 69 http://www.cbs.dtu.dk/services/Promoter/output.php | |
| 70 | |
|
17
af3174637834
Uploaded v0.2.5 preview 1, adding links to Tool Shed entry
peterjc
parents:
16
diff
changeset
|
71 This wrapper is available to install into other Galaxy Instances via the Galaxy |
|
af3174637834
Uploaded v0.2.5 preview 1, adding links to Tool Shed entry
peterjc
parents:
16
diff
changeset
|
72 Tool Shed at http://toolshed.g2.bx.psu.edu/view/peterjc/tmhmm_and_signalp |
| 7 | 73 </help> |
| 74 </tool> |