# HG changeset patch # User peterjc # Date 1378893324 14400 # Node ID fafc7f75d7ed19774bd4070c3c233bb7523efb4a # Parent f3a61c2cf309b52d2da723eb25d40b1e592d6d41 Uploaded v0.0.8 preview 8, renamed README file, MIT licence, citation information diff -r f3a61c2cf309 -r fafc7f75d7ed tools/blast2go/README.rst --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tools/blast2go/README.rst Wed Sep 11 05:55:24 2013 -0400 @@ -0,0 +1,198 @@ +Galaxy wrapper for Blast2GO for pipelines, b2g4pipe +=================================================== + +This wrapper is copyright 2011-2013 by Peter Cock, The James Hutton Institute +(formerly SCRI, Scottish Crop Research Institute), UK. All rights reserved. +See the licence text below (MIT licence). + +This is a wrapper for the command line Java tool b2g4pipe v2.5, +Blast2GO for pipelines. It is available from the Galaxy Tool Shed at: +http://toolshed.g2.bx.psu.edu/view/peterjc/blast2go + + +References +========== + +Peter Cock, Bjoern Gruening, Konrad Paszkiewicz and Leighton Pritchard (2013). +Galaxy tools and workflows for sequence analysis with applications +in molecular plant pathology. PeerJ 1:e167 +http://dx.doi.org/10.7717/peerj.167 + +S. Götz et al. (2008). +High-throughput functional annotation and data mining with the Blast2GO suite. +Nucleic Acids Res. 36(10):3420–3435. +http://dx.doi.org/10.1093/nar/gkn176 + +A. Conesa and S. Götz (2008). +Blast2GO: A Comprehensive Suite for Functional Analysis in Plant Genomics. +International Journal of Plant Genomics. 619832. +http://dx.doi.org/10.1155/2008/619832 + +A. Conesa et al. (2005). +Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research. +Bioinformatics 21:3674-3676. +http://dx.doi.org/10.1093/bioinformatics/bti610 + +See also http://www.blast2go.org/ + + +Automated Installation +====================== + +Installation via the Galaxy Tool Shed should take care of the Galaxy side of +things, including the dependency on 'blast_datatypes' which defines the +'blastxml' file format. However, you will also probably need to configure +the Blast2GO property file(s), for example if you have a local Blast2GO +database (which we recommend for speed). + + +Manual Installation +=================== + +The main dependency is b2g4pipe which must be installed manually. Also we +strongly recommend installing a local Blast2GO database as well (see the +intructions below about the blast2go.loc file). At the time of writing, +the current version is b2g4pipe v2.5 which is available here: + +* http://www.blast2go.com/data/blast2go/b2g4pipe_v2.5.zip + +You can change the path by setting the B2G4PIP environement variable to +the desired folder, but by default the script looks for the JAR file here:: + + /opt/b2g4pipe_v2.5/blast2go.jar + +To install the wrapper manually, first install 'blast_datatypes', then +copy or move the following files under the Galaxy tools folder, e.g. in a +tools/blast2go/ folder: + +* blast2go.xml (the Galaxy tool definition) +* blast2go.py (the Python wrapper script) +* README.rst (this file) + +For a manual installation of the wrapper you will also need to modify the +tools_conf.xml file to tell Galaxy to offer the tool. We suggest putting +it next to the NCBI BLAST+ wrappers. Just add the line:: + + + +If you wish to run the unit tests, also add this to tools_conf.xml.sample +and move/copy the test-data files under Galaxy's test-data folder. Then:: + + $ ./run_functional_tests.sh -id blast2go + + +Configuration +============= + +As part of setting up b2g4pipe you will need to setup one or more Blast2GO +property files which tell the tool which database to use etc. The example +b2gPipe.properties provided with b2g4pipe is often out of date. The current +server IP address and database name may given on the Blast2GO website, or +can be found by running the latest GUI version via Java web-start, and +looking under the tools/options menu. These property files can be anywhere +accessable to the Galaxy Unix user, we put them with the JAR file etc. + +You must tell Galaxy about these Blast2GO property files so that they can be +offered to the user. Copy file blast2go.loc.sample to tool-data/blast2go.loc +under the Galaxy folder and edit this to match your installation. This must +be plain text, tab separated, with three columns: + +1. ID for the setup, e.g. Spain_2012_August +2. Description for the setup, e.g. Database in Spain (August 2012) +3. Properties filename for the setup, e.g. Spain_2012_August.properties + relative to the main JAR file, or with a full path + e.g. /opt/b2g4pipe/Spain_2012_August.properties + +Avoid including "Blast2GO" in the description (column 2) as this text will be +included in the automatically assigned output dataset name. The blast2go.loc +file allows you to customise the database setup. If for example you have a local +Blast2GO server running (which we recommend for speed), and you want this to be +the default setting, include it as the first line in your blast2go.loc file. + +Consult the Blast2GO documentation for details about the property files and +setting up a local MySQL Blast2GO database. + + +History +======= + +======= ====================================================================== +Version Changes +------- ---------------------------------------------------------------------- +v0.0.1 - Initial public release +v0.0.2 - Documentation clarifications, e.g. concatenated BLAST XML is allowed. + - Fixed error handler in wrapper script (for when b2g4pipe fails). + - Reformats the XML to use old NCBI-style concatenated BLAST XML since + b2g4pipe crashes with heap space error on with large files using + current NCBI output. +v0.0.3 - Include sample loc file, tool-data/blast2go.loc.sample +v0.0.4 - Include repository_dependencies.xml file for 'blastxml' format + (previously included in the core Galaxy installation) +v0.0.5 - Quote arguments in case of spaces in filenames (internal change) + - Last release supporting b2g4pipe v2.3.5 +v0.0.6 - Support for b2g4pipe v2.5 instead of v2.3.5 + - Now invoked with a class path and es.blast2go.prog.B2GAnnotPipe + rather then simply calling the jar file + - Now uses the switch -annot instead of -a (this change breaks + support for b2g4pipe v2.3.5 unfortunately) + - Catch a few error messages and treat them explicitly as errors. +v0.0.7 - Update output description in XML file (b2g4pipe v2.3.5 included + the sequence description, b2g4pipe v2.5 omits this). +v0.0.8 - Automated installation via the Galaxy Tool Shed. + - Added unit test. + - Explain how to load the tabular file into the Blast2GO GUI. + - Link to Tool Shed added to help text and this documentation. + - Switch to standard MIT licence. + - Use reStructuredText for this README file. + - Updated citation information (Cock et al. 2013). +======= ====================================================================== + + +Developers +========== + +This script and related tools are being developed on the following hg branch: +http://bitbucket.org/peterjc/galaxy-central/src/tools + +For making the "Galaxy Tool Shed" http://toolshed.g2.bx.psu.edu/ tarball I use +the following command from the Galaxy root folder:: + + $ tar -czf blast2go.tar.gz tools/blast2go/README.rst tools/blast2go/blast2go.xml tools/blast2go/blast2go.py tools/blast2go/repository_dependencies.xml tools/blast2go/tool_dependencies.xml tool-data/blast2go.loc.sample test-data/blastp_sample.xml test-data/blastp_sample.blast2go.tabular + +Check this worked:: + + $ tar -tzf blast2go.tar.gz + tools/blast2go/README.rst + tools/blast2go/blast2go.xml + tools/blast2go/blast2go.py + tools/blast2go/repository_dependencies.xml + tools/blast2go/tool_dependencies.xml + tool-data/blast2go.loc.sample + test-data/blastp_sample.xml + test-data/blastp_sample.blast2go.tabular + + +Licence (MIT) +============= + +Permission is hereby granted, free of charge, to any person obtaining a copy +of this software and associated documentation files (the "Software"), to deal +in the Software without restriction, including without limitation the rights +to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +copies of the Software, and to permit persons to whom the Software is +furnished to do so, subject to the following conditions: + +The above copyright notice and this permission notice shall be included in +all copies or substantial portions of the Software. + +THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN +THE SOFTWARE. + + +NOTE: This is the licence for the Galaxy Wrapper only. Blast2GO and +associated data files are available and licenced separately. diff -r f3a61c2cf309 -r fafc7f75d7ed tools/blast2go/blast2go.txt --- a/tools/blast2go/blast2go.txt Thu May 23 04:38:17 2013 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,183 +0,0 @@ -Galaxy wrapper for Blast2GO for pipelines, b2g4pipe -=================================================== - -This wrapper is copyright 2011-2013 by Peter Cock, The James Hutton Institute -(formerly SCRI, Scottish Crop Research Institute), UK. All rights reserved. -See the licence text below. - -This is a wrapper for the command line Java tool b2g4pipe v2.5, -Blast2GO for pipelines. It is available from the Galaxy Tool Shed at: -http://toolshed.g2.bx.psu.edu/view/peterjc/blast2go - -See also: - -S. Götz et al. -High-throughput functional annotation and data mining with the Blast2GO suite. -Nucleic Acids Res. 36(10):3420–3435, 2008. -http://dx.doi.org/10.1093/nar/gkn176 - -A. Conesa and S. Götz. -Blast2GO: A Comprehensive Suite for Functional Analysis in Plant Genomics. -Int. J. Plant Genomics. 619832, 2008. -http://dx.doi.org/10.1155/2008/619832 - -A. Conesa et al. -Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research. -Bioinformatics 21:3674-3676, 2005. -http://dx.doi.org/10.1093/bioinformatics/bti610 - -http://www.blast2go.org/ - - -Automated Installation -====================== - -Installation via the Galaxy Tool Shed should take care of the Galaxy side of -things, including the dependency on 'blast_datatypes' which defines the -'blastxml' file format. However, you will also probably need to configure -the Blast2GO property file(s), for example if you have a local Blast2GO -database (which we recommend for speed). - - -Manual Installation -=================== - -The main dependency is b2g4pipe which must be installed manually. Also we -strongly recommend installing a local Blast2GO database as well (see the -intructions below about the blast2go.loc file). At the time of writing, -the current version is b2g4pipe v2.5 which is available here: - -http://www.blast2go.com/data/blast2go/b2g4pipe_v2.5.zip - -You can change the path by setting the B2G4PIP environement variable to -the desired folder, but by default the script looks for the JAR file here: - -/opt/b2g4pipe_v2.5/blast2go.jar - -To install the wrapper manually, first install 'blast_datatypes', then -copy or move the following files under the Galaxy tools folder, e.g. in a -tools/blast2go/ folder: - -* blast2go.xml (the Galaxy tool definition) -* blast2go.py (the Python wrapper script) -* blast2go.txt (this README file) - -For a manual installation of the wrapper you will also need to modify the -tools_conf.xml file to tell Galaxy to offer the tool. We suggest putting -it next to the NCBI BLAST+ wrappers. Just add the line: - - - -If you wish to run the unit tests, also add this to tools_conf.xml.sample -and move/copy the test-data files under Galaxy's test-data folder. Then: - -$ ./run_functional_tests.sh -id blast2go - - -Configuration -============= - -As part of setting up b2g4pipe you will need to setup one or more Blast2GO -property files which tell the tool which database to use etc. The example -b2gPipe.properties provided with b2g4pipe is often out of date. The current -server IP address and database name may given on the Blast2GO website, or -can be found by running the latest GUI version via Java web-start, and -looking under the tools/options menu. These property files can be anywhere -accessable to the Galaxy Unix user, we put them with the JAR file etc. - -You must tell Galaxy about these Blast2GO property files so that they can be -offered to the user. Copy file blast2go.loc.sample to tool-data/blast2go.loc -under the Galaxy folder and edit this to match your installation. This must -be plain text, tab separated, with three columns: - -(1) ID for the setup, e.g. Spain_2012_August -(2) Description for the setup, e.g. Database in Spain (August 2012) -(3) Properties filename for the setup, e.g. Spain_2012_August.properties - relative to the main JAR file, or with a full path - e.g. /opt/b2g4pipe/Spain_2012_August.properties - -Avoid including "Blast2GO" in the description (column 2) as this text will be -included in the automatically assigned output dataset name. The blast2go.loc -file allows you to customise the database setup. If for example you have a local -Blast2GO server running (which we recommend for speed), and you want this to be -the default setting, include it as the first line in your blast2go.loc file. - -Consult the Blast2GO documentation for details about the property files and -setting up a local MySQL Blast2GO database. - - -History -======= - -v0.0.1 - Initial public release -v0.0.2 - Documentation clarifications, e.g. concatenated BLAST XML is allowed. - - Fixed error handler in wrapper script (for when b2g4pipe fails). - - Reformats the XML to use old NCBI-style concatenated BLAST XML since - b2g4pipe crashes with heap space error on with large files using - current NCBI output. -v0.0.3 - Include sample loc file, tool-data/blast2go.loc.sample -v0.0.4 - Include repository_dependencies.xml file for 'blastxml' format - (previously included in the core Galaxy installation) -v0.0.5 - Quote arguments in case of spaces in filenames (internal change) - - Last release supporting b2g4pipe v2.3.5 -v0.0.6 - Support for b2g4pipe v2.5 instead of v2.3.5 - - Now invoked with a class path and es.blast2go.prog.B2GAnnotPipe - rather then simply calling the jar file - - Now uses the switch -annot instead of -a (this change breaks - support for b2g4pipe v2.3.5 unfortunately) - - Catch a few error messages and treat them explicitly as errors. -v0.0.7 - Update output description in XML file (b2g4pipe v2.3.5 included - the sequence description, b2g4pipe v2.5 omits this). -v0.0.8 - Automated installation via the Galaxy Tool Shed. - - Added unit test. - - Explain how to load the tabular file into the Blast2GO GUI. - - Link to Tool Shed added to help text and this documentation. - - -Developers -========== - -This script and related tools are being developed on the following hg branch: -http://bitbucket.org/peterjc/galaxy-central/src/tools - -For making the "Galaxy Tool Shed" http://toolshed.g2.bx.psu.edu/ tarball I use -the following command from the Galaxy root folder: - -$ tar -czf blast2go.tar.gz tools/blast2go/blast2go.xml tools/blast2go/blast2go.py tools/blast2go/blast2go.txt tools/blast2go/repository_dependencies.xml tools/blast2go/tool_dependencies.xml tool-data/blast2go.loc.sample test-data/blastp_sample.xml test-data/blastp_sample.blast2go.tabular - -Check this worked: - -$ tar -tzf blast2go.tar.gz -tools/blast2go/blast2go.xml -tools/blast2go/blast2go.py -tools/blast2go/blast2go.txt -tools/blast2go/repository_dependencies.xml -tools/blast2go/tool_dependencies.xml -tool-data/blast2go.loc.sample -test-data/blastp_sample.xml -test-data/blastp_sample.blast2go.tabular - - -Licence (MIT/BSD style) -======================= - -Permission to use, copy, modify, and distribute this software and its -documentation with or without modifications and for any purpose and -without fee is hereby granted, provided that any copyright notices -appear in all copies and that both those copyright notices and this -permission notice appear in supporting documentation, and that the -names of the contributors or copyright holders not be used in -advertising or publicity pertaining to distribution of the software -without specific prior permission. - -THE CONTRIBUTORS AND COPYRIGHT HOLDERS OF THIS SOFTWARE DISCLAIM ALL -WARRANTIES WITH REGARD TO THIS SOFTWARE, INCLUDING ALL IMPLIED -WARRANTIES OF MERCHANTABILITY AND FITNESS, IN NO EVENT SHALL THE -CONTRIBUTORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY SPECIAL, INDIRECT -OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER RESULTING FROM LOSS -OF USE, DATA OR PROFITS, WHETHER IN AN ACTION OF CONTRACT, NEGLIGENCE -OR OTHER TORTIOUS ACTION, ARISING OUT OF OR IN CONNECTION WITH THE USE -OR PERFORMANCE OF THIS SOFTWARE. - -NOTE: This is the licence for the Galaxy Wrapper only. Blast2GO and -associated data files are available and licenced separately. diff -r f3a61c2cf309 -r fafc7f75d7ed tools/blast2go/blast2go.xml --- a/tools/blast2go/blast2go.xml Thu May 23 04:38:17 2013 -0400 +++ b/tools/blast2go/blast2go.xml Wed Sep 11 05:55:24 2013 -0400 @@ -86,22 +86,30 @@ **References** -S. Götz et al. +If you use this Galaxy tool in work leading to a scientific publication please +cite the following papers: + +Peter Cock, Bjoern Gruening, Konrad Paszkiewicz and Leighton Pritchard (2013). +Galaxy tools and workflows for sequence analysis with applications +in molecular plant pathology. PeerJ 1:e167 +http://dx.doi.org/10.7717/peerj.167 + +S. Götz et al. (2008). High-throughput functional annotation and data mining with the Blast2GO suite. -Nucleic Acids Res. 36(10):3420–3435, 2008. +Nucleic Acids Res. 36(10):3420–3435. http://dx.doi.org/10.1093/nar/gkn176 -A. Conesa and S. Götz. +A. Conesa and S. Götz (2008). Blast2GO: A Comprehensive Suite for Functional Analysis in Plant Genomics. -Int. J. Plant Genomics. 619832, 2008. +International Journal of Plant Genomics. 619832. http://dx.doi.org/10.1155/2008/619832 -A. Conesa et al. +A. Conesa et al. (2005). Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research. -Bioinformatics 21:3674-3676, 2005. +Bioinformatics 21:3674-3676. http://dx.doi.org/10.1093/bioinformatics/bti610 -http://www.blast2go.com/ +See also http://www.blast2go.com/ This wrapper is available to install into other Galaxy Instances via the Galaxy Tool Shed at http://toolshed.g2.bx.psu.edu/view/peterjc/blast2go diff -r f3a61c2cf309 -r fafc7f75d7ed tools/blast2go/repository_dependencies.xml --- a/tools/blast2go/repository_dependencies.xml Thu May 23 04:38:17 2013 -0400 +++ b/tools/blast2go/repository_dependencies.xml Wed Sep 11 05:55:24 2013 -0400 @@ -1,5 +1,5 @@ - + - \ No newline at end of file +