diff dexseq.R @ 1:bc7eab5753a8 draft

Uploaded

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/dexseq.R	Fri Aug 28 08:42:02 2015 -0400
@@ -0,0 +1,108 @@
+## Setup R error handling to go to stderr
+options( show.error.messages=F, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } )
+# we need that to not crash galaxy with an UTF8 error on German LC settings.
+Sys.setlocale("LC_MESSAGES", "en_US.UTF-8")
+
+library("DEXSeq")
+library('getopt')
+library('rjson')
+
+
+options(stringAsfactors = FALSE, useFancyQuotes = FALSE)
+args <- commandArgs(trailingOnly = TRUE)
+
+#get options, using the spec as defined by the enclosed list.
+#we read the options from the default: commandArgs(TRUE).
+spec = matrix(c(
+    'verbose', 'v', 2, "integer",
+    'help', 'h', 0, "logical",
+    'gtf', 'a', 1, "character",
+    'outfile', 'o', 1, "character",
+    'report', 'r', 1, "character",
+    'factors', 'f', 1, "character",
+    'threads', 'p', 1, "integer",
+    'fdr', 'c', 1, "double",
+), byrow=TRUE, ncol=4);
+opt = getopt(spec);
+
+setwd(opt$report)
+
+# if help was asked for print a friendly message
+# and exit with a non-zero error code
+if ( !is.null(opt$help) ) {
+    cat(getopt(spec, usage=TRUE));
+    q(status=1);
+}
+
+trim <- function (x) gsub("^\\s+|\\s+$", "", x)
+opt$samples <- trim(opt$samples)
+opt$factors <- trim(opt$factors)
+
+parser <- newJSONParser()
+parser$addData( opt$factors )
+factorsList <- parser$getObject()
+
+sampleTable<-data.frame()
+countFiles<-c()
+factorNames<-c()
+primaryFactor<-""
+for(factor in factorsList){
+    factorName<-factor[[1]]
+    factorNames<-append(factorNames, paste(factorName,"exon",sep=":"))
+    factorValuesMapList<-factor[[2]]
+    c = length(factorValuesMapList)
+    for (factorValuesMap in factorValuesMapList){
+        for(files in factorValuesMap){
+            for(file in files){
+                if(primaryFactor == "") {
+                    countFiles<-append(countFiles,file)
+                }
+                sampleTable[basename(file),factorName]<-paste(c,names(factorValuesMap),sep="_")
+            }
+        }
+        c = c-1
+    }
+    if(primaryFactor == ""){
+        primaryFactor <- factorName
+    }
+}
+
+factorNames<-append(factorNames,"exon")
+factorNames<-append(factorNames,"sample")
+factorNames<-rev(factorNames)
+formulaFullModel <- as.formula(paste("", paste(factorNames, collapse=" + "), sep=" ~ "))
+factorNames <- head(factorNames,-1)
+formulaReducedModel <- as.formula(paste("", paste(factorNames, collapse=" + "), sep=" ~ "))
+
+sampleTable
+formulaFullModel
+formulaReducedModel
+primaryFactor
+countFiles
+opt$report
+file.path(opt$report,"DEXSeq_analysis.RData")
+getwd()
+
+dxd = DEXSeqDataSetFromHTSeq(countFiles, sampleData=sampleTable, design= formulaFullModel, flattenedfile=opt$gtf)
+
+colData(dxd)
+dxd <- estimateSizeFactors(dxd)
+sizeFactors(dxd)
+BPPARAM=MulticoreParam(workers=opt$threads)
+dxd <- estimateDispersions(dxd, formula=formulaFullModel, BPPARAM=BPPARAM)
+dxd <- testForDEU(dxd, reducedModel=formulaReducedModel, fullModel=formulaFullModel, BPPARAM=BPPARAM)
+dxd <- estimateExonFoldChanges(dxd, fitExpToVar=primaryFactor, BPPARAM=BPPARAM)
+res <- DEXSeqResults(dxd)
+table(res$padj <= opt$fdr)
+resSorted <- res[order(res$padj),]
+head(resSorted)
+
+write.csv(as.data.frame(resSorted), file=opt$outfile)
+
+if ( !is.null(opt$report) ) {
+    save(dxd, resSorted, file = file.path(opt$report,"DEXSeq_analysis.RData"))
+    save.image()
+    DEXSeqHTML(res, FDR=opt$fdr, color=c("#C3EEE7","#B7FEA0","#F1E7A1","#CEAEFF","#FF8F43","#EDC3C5","#AAA8AA","#FF0000","#637EE9","#FBFBFB"), BPPARAM=BPPARAM)
+    unlink(file.path(opt$report,"DEXSeq_analysis.RData"))
+}
+sessionInfo()