Mercurial > repos > nml > quasitools
diff complexity_bam.xml @ 0:1f1214983a1c draft default tip
planemo upload for repository https://github.com/phac-nml/quasitools commit 5a9e4c9a582828654893166caf20576f5e0c418e
author | nml |
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date | Mon, 20 Jun 2022 20:05:57 +0000 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/complexity_bam.xml Mon Jun 20 20:05:57 2022 +0000 @@ -0,0 +1,50 @@ +<tool id="complexity_bam" name="Complexity BAM" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@"> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"/> + <command detect_errors="exit_code"><![CDATA[ + + ln -s $input_bam '${input_bam.name}' && + ln -s $input_bam.metadata.bam_index '${input_bam.name}.bai' && + + quasitools complexity bam '$ref_file' '${input_bam.name}' $k -f '$filter_size' -o output.csv + + ]]></command> + <inputs> + <param name="ref_file" type="data" format="fasta" optional="false" label="Reference file" /> + <param name="input_bam" type="data" format="bam" optional="false" label="BAM file" /> + <param name="k" type="integer" label="k-mer size" value="0" min="0" /> + <param name="filter_size" type="integer" label="Filter size" value="0" min="0" /> + </inputs> + <outputs> + <data format="csv" name="output" from_work_dir="output.csv" /> + </outputs> + <tests> + <test> + <param name="ref_file" value="generated.fasta" /> + <param name="input_bam" value="generated.bam" /> + <param name="k" value="200" /> + <param name="filter_size" value="0" /> + <output name="output" > + <assert_contents> + <has_text text="Position" /> + <has_text text="0" /> + </assert_contents> + </output> + </test> + </tests> + + <help><![CDATA[ +Quasispecies Complexity +======================= + +Calculates various quasispecies complexity measures on next generation sequenced data from a BAM file and it's corresponding reference file. + +The measures of complexity are taken from the following work: +Gregori, Josep, et al. "Viral quasispecies complexity measures." Virology 493 (2016): 227-237. + + +]]></help> + <expand macro="citations" /> +</tool>