Mercurial > repos > nilesh > rseqc

annotate read_hexamer.xml @ 7:d06c7d4fdc29 draft

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit a63ead9b3f0c781032beabf8bed2c65445500291
author iuc
date Tue, 18 Sep 2018 05:21:29 -0400
parents 71ed55a3515a
children d7f6b3653d84
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1 <tool id="rseqc_read_hexamer" name="Hexamer frequency" version="@WRAPPER_VERSION@">
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2 <description>
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3 calculates hexamer (6mer) frequency for reads, genomes, and mRNA sequences
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4 </description>
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6 <macros>
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7 <import>rseqc_macros.xml</import>
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8 </macros>
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10 <expand macro="requirements" />
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12 <expand macro="stdio" />
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14 <version_command><![CDATA[read_hexamer.py --version]]></version_command>
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16 <command><![CDATA[
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17 #import re
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18 #set $input_list = []
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19 #for $i, $input in enumerate($inputs):
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21 #set $safename = re.sub('[^\w\-_]', '_', $input.element_identifier)
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22 #if $safename in $input_list:
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23 #set $safename = str($safename) + "." + str($i)
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24 #end if
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25 $input_list.append($safename)
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27 #if $input.is_of_type("fastq.gz", "fastqsanger.gz"):
71ed55a3515a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
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28 gunzip -c '${input}' > "${safename}" &&
71ed55a3515a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
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29 #else:
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30 ln -sf '${input}' "${safename}" &&
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31 #end if
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32 #end for
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33 read_hexamer.py -i '${ ','.join( [ $name for $name in $input_list ] ) }'
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34 #if $refgenome:
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35 -r '${refgenome}'
71ed55a3515a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
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36 #end if
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37 #if $refgene:
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38 -g '${refgene}'
71ed55a3515a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
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39 #end if
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40 > '${output}'
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41 ]]>
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42 </command>
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44 <inputs>
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45 <param name="inputs" type="data" label="Read sequences in fasta or fastq format" format="fasta,fastq,fastqsanger,fastq.gz,fastqsanger.gz" help="(--input)" multiple="true" />
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46 <param name="refgenome" type="data" label="Reference genome seqeunce (fasta)" format="fasta" optional="true" help="(--refgenome)" />
71ed55a3515a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
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47 <param name="refgene" type="data" label="Reference mRNA sequence (fasta)" format="fasta" optional="true" help="(--refgene)" />
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48 </inputs>
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49
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50 <outputs>
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51 <data name="output" format="tabular" label="${tool.name} on ${on_string}" />
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52 </outputs>
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53
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54 <tests>
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55 <test>
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56 <param name="inputs" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq"/>
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57 <output name="output">
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58 <assert_contents>
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59 <has_line line="Hexamer&#009;pairend_strandspecific_51mer_hg19_chr1_1-100000_R1_fastq" />
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60 <has_line line="AAAAAA&#009;0.00217391304348" />
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61 </assert_contents>
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62 </output>
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63 </test>
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64 <test>
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65 <param name="inputs" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq.gz" ftype="fastqsanger.gz"/>
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66 <output name="output">
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67 <assert_contents>
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68 <has_line line="Hexamer&#009;pairend_strandspecific_51mer_hg19_chr1_1-100000_R1_fastq_gz" />
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69 <has_line line="AAAAAA&#009;0.00217391304348" />
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70 </assert_contents>
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71 </output>
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72 </test>
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73 <test>
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74 <param name="inputs" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq,pairend_strandspecific_51mer_hg19_chr1_1-100000.R2.fastq"/>
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75 <output name="output">
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76 <assert_contents>
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77 <has_line line="Hexamer&#009;pairend_strandspecific_51mer_hg19_chr1_1-100000_R1_fastq&#009;pairend_strandspecific_51mer_hg19_chr1_1-100000_R2_fastq" />
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78 <has_line line="AAAAAA&#009;0.00217391304348&#009;0.00534759358289" />
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79 </assert_contents>
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80 </output>
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81 </test>
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82 <test>
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83 <param name="inputs" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq,pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq"/>
71ed55a3515a planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rseqc commit 37fb1988971807c6a072e1afd98eeea02329ee83
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84 <output name="output">
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85 <assert_contents>
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86 <has_line line="Hexamer&#009;pairend_strandspecific_51mer_hg19_chr1_1-100000_R1_fastq&#009;pairend_strandspecific_51mer_hg19_chr1_1-100000_R1_fastq.1" />
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87 <has_line line="AAAAAA&#009;0.00217391304348&#009;0.00217391304348" />
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88 </assert_contents>
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89 </output>
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90 </test>
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91 <!-- Unable to test with collections at the moment (requires type="data_collection" on the input)
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92 <test>
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93 <param name="inputs">
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94 <collection type="list">
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95 <element name="read_1" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.R1.fastq" />
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96 <element name="read_2" value="pairend_strandspecific_51mer_hg19_chr1_1-100000.R2.fastq" />
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97 </collection>
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98 </param>
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99 <output name="output" file="output.read_hexamer.2.txt" />
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100 </test>
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101 -->
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102 </tests>
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103
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104 <help><![CDATA[
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105 read_hexamer.py
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106 +++++++++++++++++++++
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107
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108 Calculate hexamer (6mer) frequency. If ‘-r’ was specified, hexamer frequency
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109 is also calculated for the reference genome. If ‘-g’ was provided, hexamer
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110 frequency is also calculated for the mRNA sequences.
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111
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112 Inputs
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113 ++++++++++++++
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114
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115 Input reads file
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116 Read sequences in fasta or fastq format.
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117
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118 Reference Genome
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119 Reference genome sequence in fasta format.
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120
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121 Reference Gene
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122 Reference mRNA sequences in fasta format.
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123
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124
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125 Outputs
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126 ++++++++++++++
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127
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128 Tabular file of hexamer frequences in for each input.
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129
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130 @ABOUT@
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131
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132 ]]>
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133 </help>
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134
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135 <expand macro="citations" />
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136
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137 </tool>