# HG changeset patch # User nikos # Date 1438780862 14400 # Node ID 431aebd93843101da484b7cbe2f13c4bf253b32f # Parent fb76303acf4ffb6929ba06dd430fb1ca18009a78 Fixed a bug in k2n.R where the function k2n_calc() would result in an error for single-end read files. diff -r fb76303acf4f -r 431aebd93843 k2n.R --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/k2n.R Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,119 @@ +# Setup R error handling to go to stderr +options( show.error.messages = FALSE, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) + +# we need that to not crash galaxy with an UTF8 error on LC settings. +Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") + +options(stringAsfactors = FALSE, useFancyQuotes = FALSE) +args <- commandArgs(trailingOnly = TRUE) + +# Declare functions + +k2n_calc <- function(merged_file, unique_barcode_file, output_file, paired = TRUE) +{ + + # Read in inputs: + merged <- read.table( merged_file ) + colnames(merged) <- c("RNAid", "Start", "End", "Barcodes") + barcode_length <- max(nchar(as.character(merged$Barcodes))) + + #Single end + if( !paired) + read_counts <- as.data.frame(table(subset(merged, select = - (End:Barcodes)))) + #Paired end + else + read_counts <- as.data.frame(table(subset(merged, select = - Barcodes))) + read_counts <- read_counts[read_counts$Freq != 0, ] + + max_observed <- max(read.table(unique_barcode_file)[,4]) + + #Check if max_observed equals max possible complexity. If yes - subtract 1 + #(otherwise 'while' loop below never ends) + if( max_observed == 4**barcode_length ) { + max_observed <- max_observed - 1 + barcodes_saturated <- TRUE + } else + barcodes_saturated <- FALSE + + # Remove top-quartile of reads: + #Single end + if (!paired){ + barcodes_nt <- merged[ do.call( + paste, as.list(subset(merged, select = - (End:Barcodes)))) %in% + do.call(paste, as.list(read_counts[( + read_counts$Freq ) <= quantile(read_counts$Freq, 0.75), + c("RNAid", "Start") ] ) ) , "Barcodes" ] + } else { + barcodes_nt <- merged[ do.call( + paste, as.list(subset(merged, select = - Barcodes))) %in% + do.call(paste, as.list(read_counts[( + read_counts$Freq ) <= quantile(read_counts$Freq, 0.75), + c("RNAid", "Start", "End") ] ) ) , "Barcodes" ] + } + + # make the matrix with the nucleotide freqs per position: + nt_counts <- matrix( nrow = 4, ncol = barcode_length ) + for( h in 1:ncol( nt_counts ) ){ + j <- 1 + for( nt_local in c( "A","C","G","T" ) ) { + nt_counts[ j, h ] <- sum( substr( as.character( barcodes_nt ), h, + h) == nt_local ) + j <- j + 1 + } + } + + # Calculate frequencies + nt_freqs <- nt_counts / colSums( nt_counts ) + + nt_values <- split(nt_freqs, rep(1:ncol(nt_freqs), each = nrow(nt_freqs))) + + all_posible_comb <- expand.grid( nt_values ) + + probs <- apply( all_posible_comb, 1, prod ) + + ###Create Mf_to_Sf: + results <- c() + + i <- 1 + results[ i ] <- sum( 1 - ( 1 - probs )**i ) + j <- 1 + while( results[ i ] <= max_observed ) { + i <- i + 1 + results[ i ] <- sum( 1 - ( 1 - probs )**i ) #Mf to Sf + if(results[ i ] == results[ i - 1]){ + if(j > 2) + stop("Too low complexity to estimate k2n distribution") + else + j <- j + 1 + }else + j <- 1 + } + + #assign molecules number to unique barcode number: + k2n <- c() + for( i in 1:floor( max( results ) ) ) { + abs( results - i ) -> difference + + #if you want to know how many molecules underlie n unique barcodes + #ask k2n[n] + k2n[ i ] <- which( difference == min( difference ) ) + } + + #Assign +Inf for fragments which have saturated the barcodes + #(see correct_oversaturation function): + if( barcodes_saturated ) + k2n[max_observed + 1] <- Inf + + if(!missing(output_file)) { + write(k2n, file = output_file ) + } else + k2n +} + +# Read inputs +merged <- args[1] +unique_barcodes <- args[2] +output <- args[3] +paired_check <- as.logical(args[4]) + +k2n_calc( merged, unique_barcodes, output, paired = paired_check) diff -r fb76303acf4f -r 431aebd93843 normalize.R --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/normalize.R Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,169 @@ +##!/usr/bin/Rscript + +## Setup R error handling to go to stderr +options( show.error.messages = FALSE, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) + +# we need that to not crash galaxy with an UTF8 error on LC settings. +Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") + +suppressMessages(library('getopt')) + +#get options, using the spec as defined by the enclosed list. +#we read the options from the default: commandArgs(TRUE). +spec = matrix(c( + 'treated', 't', 1, "character", + 'control', 'c', 2, "character", + 'method', 'm', 1, "character", + 'fuComplexity', 'b', 2, "integer", + 'k2nTreated', 'k', 2, "character", + 'k2nControl', 'a', 2, "character", + 'cutoff', 'g', 1, "integer", + 'reference', 'r', 2, "character", + 'compdata', 'h', 2, "character", + 'dtcr', 'd', 0, "logical", + 'dtcrWindow', 'e', 2, "integer", + 'dtcrToZero', 'f', 2, "character", + 'slograt', 's', 0, "logical", + 'slogratWindow', 'p', 2, "integer", + 'depthCorrection', 'q', 2, "character", + 'pseudocount', 'l', 2, "integer", + 'swinsor', 'w', 0, "logical", + 'swinsorWindow', 'x', 2, "integer", + 'winsorLevel', 'y', 2, "double", + 'fixQuantile', 'z', 2, "character", + 'ntOffset', 'n', 2, "integer", + 'outputDir', 'o', 1, "character", + 'bedgraph', 'v', 2, "character", + 'bed', 'j', 2, "character", + 'genome', 'i', 2, "character", + 'trackName', 'u', 2, "character" +), byrow=TRUE, ncol=4); + +opt = getopt(spec); + +suppressMessages(require(RNAprobR)) + + +#Create output dir +dir.create(opt$outputDir, showWarnings = FALSE, recursive = TRUE) + + +if (opt$method=="counts"){ + treated_euc <- readsamples(opt$treated, euc=opt$method) +} else if (opt$method=="Fu") { + treated_euc <- readsamples(opt$treated, euc=opt$method, m = opt$fuComplexity) +} else { + treated_euc <- readsamples(opt$treated, euc=opt$method, k2n_files=opt$k2nTreated) +} + + +if ( !is.null(opt$reference)){ + comp_treated <- comp(treated_euc, cutoff=opt$cutoff, + fasta_file=opt$reference) +} else { + comp_treated <- comp(treated_euc, cutoff=opt$cutoff) +} + + +#If present, read control file +if ( !is.null(opt$control) && opt$control != 'None'){ + if (opt$method=="counts"){ + control_euc <- readsamples(opt$control, euc=opt$method) + } else if (opt$method=="Fu") { + control_euc <- readsamples(opt$control, euc=opt$method, m = opt$fuComplexity) + } else { + control_euc <- readsamples(opt$control, euc=opt$method, k2n_files=opt$k2nControl) + } + if ( !is.null(opt$reference)){ + comp_control <- comp(control_euc, cutoff=opt$cutoff, + fasta_file=opt$reference) + } else { + comp_control <- comp(control_euc, cutoff=opt$cutoff) + } +} + +#compdata +if ( !is.null(opt$compdata) ) { + + normalized <- compdata(comp_treated, nt_offset=opt$ntOffset) + colnames(mcols(normalized))[1:4] <- paste(colnames(mcols(normalized))[1:4], ".treated", sep="") + + if ( !is.null(opt$control) && opt$control != 'None') { + normalized <- compdata(comp_control, nt_offset=opt$ntOffset, add_to=normalized) + colnames(mcols(normalized))[6:9] <- c("TC.control", "TCR.control", "Cover.control", "PC.control") + } +} + +#dtcr +if ( !is.null(opt$dtcr) ) { + if ( exists("normalized")) { + normalized <- dtcr(comp_control, comp_treated, window_size=opt$dtcrWindow, + nt_offset=opt$ntOffset, bring_to_zero=opt$dtcrToZero, + add_to=normalized) + } + else { + normalized <- dtcr(comp_control, comp_treated, window_size=opt$dtcrWindow, + nt_offset=opt$ntOffset, bring_to_zero=opt$dtcrToZero) + } +} + +#slograt +if ( !is.null(opt$slograt) ) { + if ( exists("normalized")) { + normalized <- slograt(comp_control, comp_treated, window_size=opt$slogratWindow, + nt_offset=opt$ntOffset, depth_correction = opt$depthCorrection, + pseudocount=opt$pseudocount, add_to=normalized) + } + else { + normalized <- slograt(comp_control, comp_treated, window_size=opt$slogratWindow, + nt_offset=opt$ntOffset, depth_correction = opt$depthCorrection, + pseudocount=opt$pseudocount) + } +} + +#swinsor +if ( !is.null(opt$swinsor) ) { + if ( exists("normalized")) { + normalized <- swinsor(comp_treated, winsor_level = opt$winsorLevel, + window_size=opt$swinsorWindow, + only_top=opt$fixQuantile, nt_offset=opt$ntOffset, + add_to=normalized) + } + else { + normalized <- swinsor(comp_treated, winsor_level = opt$winsorLevel, + window_size=opt$swinsorWindow, + only_top=opt$fixQuantile, nt_offset=opt$ntOffset) + } +} + +#bedgraph output +if ( !is.null(opt$bedgraph)) { + if ( !is.null(opt$dtcr)){ + norm2bedgraph(normalized, bed_file = opt$bed, norm_method = "dtcr", + genome_build = opt$genome, + bedgraph_out_file = paste(opt$outputDir, "/dtcr", + sep=""), + track_name = opt$trackName, + track_description = opt$trackDesc) + } + if ( !is.null(opt$slograt)){ + norm2bedgraph(normalized, bed_file = opt$bed, norm_method = "slograt", + genome_build = opt$genome, + bedgraph_out_file = paste(opt$outputDir, "/slograt", + sep=""), + track_name = opt$trackName, + track_description = opt$trackDesc) + } + if ( !is.null(opt$swinsor)){ + norm2bedgraph(normalized, bed_file = opt$bed, norm_method = "swinsor", + genome_build = opt$genome, + bedgraph_out_file = paste(opt$outputDir, "/swinsor", + sep=""), + track_name = opt$trackName, + track_description = opt$trackDesc) + } +} + +output <- GR2norm_df(normalized) +write.table( output, paste(opt$outputDir, "/norm_df.txt", sep = ""), sep = "\t", + quote = F, row.names = F) diff -r fb76303acf4f -r 431aebd93843 normalize.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/normalize.xml Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,248 @@ + + RNA probing signal + + R_3_1_1 + RNAprobR + RNAprobR + RNA_PROBING_SCRIPT_PATH + + + + normalize.R + + -t $treated + -c $control + + --method $euc_method.euc + + #if str($euc_method.euc) == "Fu": + --fuComplexity $euc_method.complexity + #else if str($euc_method.euc) == "HRF-Seq": + --k2nTreated $euc_method.k2n_treated + --k2nControl $euc_method.k2n_control + #end if + + --reference $fasta + + #if str($compdata) == 'yes': + --compdata + #end if + + #if str($dtcr.check) == 'yes': + --dtcr + --dtcrWindow $dtcr.wsize + --dtcrToZero $dtcr.zero + #end if + + #if str($slograt.check) == 'yes': + --slograt + --slogratWindow $slograt.wsize + --depthCorrection $slograt.depth_cor + --pseudocount $slograt.pseudocount + #end if + + #if str($swinsor.check) == 'yes': + --swinsor + --swinsorWindow $swinsor.wsize + --winsorLevel $swinsor.winsor_level + --fixQuantile $swinsor.only_top + #end if + + --ntOffset $nt_offset + --cutoff $cutoff + + #if str($bedgraph.check) == 'yes': + --bedgraph + --bed "$bedgraph.bed_file" + --genome "$bedgraph.genome" + --trackName "$bedgraph.track_name" + #end if + + -o 'output_dir' + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + bedgraph['check'] == 'yes' and dtcr['check'] == 'yes' + + + + + + + + + + + bedgraph['check'] == 'yes' and slograt['check'] == 'yes' + + + + + + + + + + + bedgraph['check'] == 'yes' and swinsor['check'] == 'yes' + + + + + + + + + + + + + + + +**What it does** + +Normalize tool performs termini based signal detection using summarized counts of sequenced reads (**Read Counts**) or sets of reads carrying the same barcode seqeuence (**Unique Barcodes**). The number of unique barcodes can be converted to Estimated Unique Counts (EUCs) by correcting for the existence of distinct barcodes of the same sequence with (Fu et al., 2011) or without (Kielpinski and Vinther, 2014) the assumption that all barcodes have the same probability of being captured. + +------ + +**Inputs** + +**Unique Barcodes** or **Read Counts** files produced by *Summarize Unique Barcodes* tool for treated and control samples. If deltaTCR of Smooth Log2 Ratio is desired a Control files are required. + +**k2n file(s)** is (are) required if "HRF-Seq" is selected as the "EUC" method. + +**Reference sequences** in FASTA format. Use the file that you produced the read alignment with. + +------ + +**EUC method** + + * **Counts** - Preserves the count of unique barcodes or read counts (depending on the input). It is the only suitable method for reading-in “Read Counts” file and an applicable method when the highest observed unique barcode count is lower than the square root of the count of all possible barcode combinations (Casbon et al., 2011). + + * **Fu** - Allows different barcodes to share the same sequence and assumes equal probability of ligating each barcode sequence (Fu et al., 2011). When selected the value of the random barcode complexity must be specified (e.g. barcode signature NWTRYSNNNN complexity equals 4×2×1×2×2×2×4×4×4×4 = 16384). + + * **HRF-Seq** - Similar to "Fu" but the probability of ligating different barcodes is estimated via observed frequencies of nucleotides at each barcode position (Kielpinski and Vinther, 2014). Requires k2n files for both treated and control samples. + +------ + +**Normalization methods** + +For a detailed description of the methods used read (publication pending) + +------ + +**Outputs** + +A tab seperated file with normalized signal values: + +====== ============================================================================================== +Column Description +------ ---------------------------------------------------------------------------------------------- + 1 Transcript identifier + 2 Position + 3 Nucleotide + 4- Normalized signal value followed by a column with a test significance value (where applicable) +====== ============================================================================================== + +. + +A BedGraph_ file that can be used for direct data display in the `UCSC Browser`_. + +.. _BedGraph: http://genome.ucsc.edu/goldenpath/help/bedgraph.html + +.. _UCSC Browser: http://genome.ucsc.edu/ + + + + + 10.1093/nar/gku167 + 10.1073/pnas.1017621108 + 10.1093/nar/gkr217 + + + diff -r fb76303acf4f -r 431aebd93843 plot_rna.R --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/plot_rna.R Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,41 @@ +#Setup R error handling to go to stderr +options( show.error.messages = FALSE, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) + +# we need that to not crash galaxy with an UTF8 error on German LC settings. +Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") + +suppressMessages(library('getopt')) + +#get options, using the spec as defined by the enclosed list. +#we read the options from the default: commandArgs(TRUE). +spec = matrix(c( + 'help' , 'h', 0, "logical", + 'input', 'i', 1, "character", + 'transcript', 't', 1, "character", + 'method', 'm', 2, "character", + 'cutoff', 'c', 2, "double", + 'type', 'p', 2, "character", + 'output', 'o', 1, "character" +), byrow=TRUE, ncol=4); + +opt = getopt(spec); + +suppressMessages(require(RNAprobR)) + +#Read and convert input to GRanges object +data <- read.table(opt$input, header = TRUE) +dataGR <- norm_df2GR(data) + +#Check if given transcript exists in input file +if ( ! opt$transcript %in% data$RNAid ) { stop("Transript not found. Check input file.") } + +pdf(opt$output) +for (method in strsplit(opt$method, ",")[[1]] ) { + + #Check if columns exists in data file + if (! method %in% colnames(data)) { next } + + plotRNA(dataGR, opt$transcript, method, stat_cutoff = opt$cutoff, type = opt$type, + main=paste(opt$transcript,": ", method, sep="")) +} +dev.off() diff -r fb76303acf4f -r 431aebd93843 plot_rna.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/plot_rna.xml Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,72 @@ + + + + + R_3_1_1 + RNAprobR + RNAprobR + RNA_PROBING_SCRIPT_PATH + + + + plot_rna.R -i $input -t "$transcript" -m $norm_method -c $cutoff -p $plot_type -o $plot > /dev/null + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +**What it does** + +Produces plots for a given transcript in the tabular file produced by *Normalize* tool (one plot per selected method). If a selected normalizing method is not included in the input file it simply not be plotted. + +------ + +**Example** + +Input (head):: + + RNAid Pos nt dtcr dtcr.p + 16S_rRNA_E.coli 1 A 0 0.00787957002462928 + 16S_rRNA_E.coli 2 A 0.000606618229204106 2.87738710042044e-07 + 16S_rRNA_E.coli 3 A 0.00170457733442839 0.0242502062681708 + 16S_rRNA_E.coli 4 T 0.00171914631528914 0.504983784068465 + 16S_rRNA_E.coli 5 T 0.00111252808608504 5.26073688354678e-06 + 16S_rRNA_E.coli 6 G 0.00175507669063296 9.92896700324683e-05 + +Output (Significance: 1e-08, type: histogram) + +.. image:: http://people.binf.ku.dk/slm279/plot.png + + + diff -r fb76303acf4f -r 431aebd93843 preprocessing.sh --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/preprocessing.sh Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,156 @@ +#!/bin/bash + +#################################################################################################### +#Copyright (C) 2014 Lukasz Kielpinski, Nikos Sidiropoulos + +#This program is free software: you can redistribute it and/or modify it under the terms of the +#GNU General Public License as published by the Free Software Foundation, either version 3 of the +#License, or (at your option) any later version. + +#This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without +#even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +#GNU General Public License for more details (http://www.gnu.org/licenses/). +#################################################################################################### + +#Preprocessing workflow - Read debarcoding and trimming. + +function print_help { +cat < -r -b -t -o +End-of-message +exit +} + +#defaults +output_dir="./output_dir" +read2="" +trim_length=0 + +#parse input +while getopts h1:2:b:t:o: myarg +do case "$myarg" in + h) print_help + exit ;; + 1) read1="$OPTARG" ;; #required + 2) read2="$OPTARG" ;; #optional + b) barcode="$OPTARG" ;; #optional + t) trim_length="$OPTARG" ;; #optional + o) output_dir="$OPTARG" ;; #optional + [?]) echo "ERROR: Unknown parameter" + print_help + exit 1 ;; + esac +done + +if [[ -z $read1 ]]; then + echo "ERROR: Read1 file required!" + print_help + exit 1 +fi + +# Create the output folder +mkdir -p $output_dir + +if [ -z "$barcode" ]; then + barcode='' +fi +BAR_LENGTH=`eval echo ${#barcode}` + +################################################################################################### +# Convert the Barcode signature to a regular exression. + +# Example: +# +# Barcode in the oligo used: NWTRYSNNNN +# Which means that each proper read must begin with: NNNN(C|G)(A|G)(C|T)A(A|T)N +# As regex: +# ^[ACGT][ACGT][ACGT][ACGT][CG][AG][CT][A][AT][ACGT] +# Reverse complement the barcode sequence and create a regular expression. + +REV_COMPLEMENT=$(perl -0777ne's/\n //g; tr/ATGCatgcNnYyRrKkMmBbVvDdHh/TACGtacgNnRrYyMmKkVvBbHhDd/; print scalar reverse $_;' <(echo $barcode)) + +REG_EXP=$(sed 's/A/[A]/g;s/C/[C]/g;s/G/[G]/g;s/T/[T]/g;s/R/[AG]/g;s/Y/[CT]/g;s/S/[GC]/g;s/W/[AT]/g;s/K/[GT]/g;s/M/[AC]/g;s/B/[CGT]/g;s/D/[AGT]/g;s/H/[ACT]/g;s/V/[ACG]/g;s/N/[AGCTN]/g;' <<< $REV_COMPLEMENT) + +################################################################################################### +#Remove reads that do not start with the signature (Read1) + +#First awk removes them, second awk removes corresponding quality strings. +#Remove last N nt - may be derived from the random primers. + +awk '{if(NR%4==2){if(/^'"$REG_EXP"'/){print}else{print ""}}else{print}}' $read1 | + +awk 'BEGIN{trim_flag=0; trimming_stats=0; all_processed=0} +{ + if(NR%4==1){print; all_processed++} + if(NR%4==2){if(length($1)==0){trim_flag=1;trimming_stats++}else{trim_flag=0};print} + if(NR%4==3){print} + if(NR%4==0){if(trim_flag==1){print ""}else{print $0}} +}END{print(trimming_stats, all_processed) > "trimming_stats.error"}' | + +awk -v len="${trim_length}" '{if(NR%2==0){print(substr($1,0,length($1)-len))}else{print}}' > R1.fastq & + +wait + +mv trimming_stats.error $output_dir/trimming_stats.txt + +################################################################################################### +#Trim primers and possible random barcode + +if [ -z "$read2" ]; then + #single-end + + #Extract the barcode sequence from the first read: + awk -v len="${BAR_LENGTH}" '{if(NR%2==0){print(substr($0,len+1,length($0)))}else{print($0)}}' R1.fastq | awk '{print($1)}' > $output_dir/read1.fastq & + + awk -v len="${BAR_LENGTH}" '{if(NR%4==1){print($1)}else{if(NR%4==2){print(substr($0,0,len))}}}' R1.fastq | paste - - > $output_dir/barcodes.txt & + + wait + + #Remove temp files + rm R1.fastq + +else + #paired-end + + #Trim primers (Read2) + awk -v len1="${trim_length}" -v len2="${BAR_LENGTH}" '{if(NR%2==0){print(substr($0,len1+1,(length($0)-len1-len2)))}else{print($0)}}' $read2 > R2.fastq + + ## Fix pairs in Read1 and Read2 fastq files + #Sort + awk '{print($1)}' R1.fastq | paste - - - - | awk '(NF==4){print $0}' | sort -S1G -k1,1 > R1_collapsed.fastq & + awk '{print($1)}' R2.fastq | paste - - - - | awk '(NF==4){print $0}' | sort -S1G -k1,1 > R2_collapsed.fastq & + + wait + + join -1 1 -2 1 R1_collapsed.fastq R2_collapsed.fastq > joined.fastq + + awk 'BEGIN{OFS=""}{print($1"\n"$2"\n"$3"\n"$4)}' joined.fastq > R1_sorted.fastq & + awk 'BEGIN{OFS=""}{print($1"\n"$5"\n"$6"\n"$7)}' joined.fastq > $output_dir/read2.fastq & + + wait + + rm joined.fastq R1_collapsed.fastq R2_collapsed.fastq R1.fastq R2.fastq & + + ######################################################################## + #Extract the barcode sequence from the first read: + awk -v len="${BAR_LENGTH}" '{if(NR%2==0){print(substr($0,len+1,length($0)))}else{print($0)}}' R1_sorted.fastq | awk '{print($1)}' > $output_dir/read1.fastq & + + awk -v len="${BAR_LENGTH}" '{if(NR%4==1){print($1)}else{if(NR%4==2){print(substr($0,0,len))}}}' R1_sorted.fastq | paste - - > $output_dir/barcodes.txt & + + wait + + #Remove temp files + rm R1_sorted.fastq + +fi diff -r fb76303acf4f -r 431aebd93843 preprocessing.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/preprocessing.xml Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,136 @@ + + RNA probing data + + + gnu_awk + RNA_RPOBING_SCRIPT_PATH + + + + preprocessing.sh + + ## check if paired-end + #if str( $library.type ) == "paired" + -2 $library.input2 + #end if + + ## Inputs + -1 $library.input1 + + ## Barcode sequence + -b '$library.barcode_seq' + + ## Trimming length + -t $trim + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + library['type'] == "paired" + + + + + + + + + + + + + + + + + + + +**What it does** + +*Preprocessing* tool removes and saves the random barcodes sequences, if they were ligated to 3’ ends of cDNA, in a separate dataset to be used in downstream analysis. Additionally to debarcoding, it trims 1) the 5’ end of the second-in-pair reads to remove the reverse transcription primer derived sequence and 2) 3’ end of both reads to remove possible random barcode incorporation in the second-in-pair read and random primer in first-in-pair read. + +------ + +**Examples** + +Sample input files (quality scores omited):: + + * Read1 + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 1:N:0:ATCACG + TTCGCACAACATNATGGAGGCTTCACGGTACAGAACGAGGCCAGCAAATACCAAGTCTCAGTGAACAAATACAAAGGGACGGCTGGCAACGCCCTCAT + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 1:N:0:ATCACG + ACCCCGCATCAAATTGGGAACTACTTCCAGCAGTTGTTAGACTTGGGCTCTGGCAGCCCCTTGGAGTGGAGGGACTTGCAGCCCTTCTTATCAGGTCT + + * Read2 + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 2:N:0:ATCACG + CACAAATCTGCCGTTTGGATTGGCTGCATGGCATCTGTTATACCACCAGCCACCACCATCTTCTTTGGAGCACTGTTTTCTTGGATCCGTAGTTACCC + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 2:N:0:ATCACG + GTTGGGGGTGTGGGGAAAAAAATAAAAATCGTGAGAAGTTTTAAGACTATGTCACAAAAATGGCTTTAATTATACCATCAAACAGAAACCACCAATTG + +Run 1 - Barcode Sequence = '', Trimming length = 10:: + + * Read1 + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 + TTCGCACAACATNATGGAGGCTTCACGGTACAGAACGAGGCCAGCAAATACCAAGTCTCAGTGAACAAATACAAAGGGACGGCTGGCA + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 + ACCCCGCATCAAATTGGGAACTACTTCCAGCAGTTGTTAGACTTGGGCTCTGGCAGCCCCTTGGAGTGGAGGGACTTGCAGCCCTTCT + + * Read2 + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 + CCGTTTGGATTGGCTGCATGGCATCTGTTATACCACCAGCCACCACCATCTTCTTTGGAGCACTGTTTTCTTGGATCCGTAGTTACCC + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 + TGGGGAAAAAAATAAAAATCGTGAGAAGTTTTAAGACTATGTCACAAAAATGGCTTTAATTATACCATCAAACAGAAACCACCAATTG + +Run 2 - Barcode Sequence = 'NNNNNNN', Trimming length = 10:: + + * Read1 + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 + AACATNATGGAGGCTTCACGGTACAGAACGAGGCCAGCAAATACCAAGTCTCAGTGAACAAATACAAAGGGACGGCTGGCA + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 + ATCAAATTGGGAACTACTTCCAGCAGTTGTTAGACTTGGGCTCTGGCAGCCCCTTGGAGTGGAGGGACTTGCAGCCCTTCT + + * Read2 + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 + CCGTTTGGATTGGCTGCATGGCATCTGTTATACCACCAGCCACCACCATCTTCTTTGGAGCACTGTTTTCTTGGATCCGTA + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 + TGGGGAAAAAAATAAAAATCGTGAGAAGTTTTAAGACTATGTCACAAAAATGGCTTTAATTATACCATCAAACAGAAACCA + + * Barcodes + + @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 TTCGCAC + @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 ACCCCGC + + + + + 10.1093/nar/gku167 + + + diff -r fb76303acf4f -r 431aebd93843 rna_probing/k2n.R --- a/rna_probing/k2n.R Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,119 +0,0 @@ -# Setup R error handling to go to stderr -options( show.error.messages = FALSE, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) - -# we need that to not crash galaxy with an UTF8 error on LC settings. -Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") - -options(stringAsfactors = FALSE, useFancyQuotes = FALSE) -args <- commandArgs(trailingOnly = TRUE) - -# Declare functions - -k2n_calc <- function(merged_file, unique_barcode_file, output_file, paired = TRUE) -{ - - # Read in inputs: - merged <- read.table( merged_file ) - colnames(merged) <- c("RNAid", "Start", "End", "Barcodes") - barcode_length <- max(nchar(as.character(merged$Barcodes))) - - #Single end - if( !paired) - read_counts <- as.data.frame(table(subset(merged, select = - (End:Barcodes)))) - #Paired end - else - read_counts <- as.data.frame(table(subset(merged, select = - Barcodes))) - read_counts <- read_counts[read_counts$Freq != 0, ] - - max_observed <- max(read.table(unique_barcode_file)[,4]) - - #Check if max_observed equals max possible complexity. If yes - subtract 1 - #(otherwise 'while' loop below never ends) - if( max_observed == 4**barcode_length ) { - max_observed <- max_observed - 1 - barcodes_saturated <- TRUE - } else - barcodes_saturated <- FALSE - - # Remove top-quartile of reads: - #Single end - if (!paired){ - barcodes_nt <- merged[ do.call( - paste, as.list(subset(merged, select = - (End:Barcodes)))) %in% - do.call(paste, as.list(read_counts[( - read_counts$Freq ) <= quantile(read_counts$Freq, 0.75), - c("RNAid", "Start") ] ) ) , "Barcodes" ] - } else { - barcodes_nt <- merged[ do.call( - paste, as.list(subset(merged, select = - Barcodes))) %in% - do.call(paste, as.list(read_counts[( - read_counts$Freq ) <= quantile(read_counts$Freq, 0.75), - c("RNAid", "Start", "End") ] ) ) , "Barcodes" ] - } - - # make the matrix with the nucleotide freqs per position: - nt_counts <- matrix( nrow = 4, ncol = barcode_length ) - for( h in 1:ncol( nt_counts ) ){ - j <- 1 - for( nt_local in c( "A","C","G","T" ) ) { - nt_counts[ j, h ] <- sum( substr( as.character( barcodes_nt ), h, - h) == nt_local ) - j <- j + 1 - } - } - - # Calculate frequencies - nt_freqs <- nt_counts / colSums( nt_counts ) - - nt_values <- split(nt_freqs, rep(1:ncol(nt_freqs), each = nrow(nt_freqs))) - - all_posible_comb <- expand.grid( nt_values ) - - probs <- apply( all_posible_comb, 1, prod ) - - ###Create Mf_to_Sf: - results <- c() - - i <- 1 - results[ i ] <- sum( 1 - ( 1 - probs )**i ) - j <- 1 - while( results[ i ] <= max_observed ) { - i <- i + 1 - results[ i ] <- sum( 1 - ( 1 - probs )**i ) #Mf to Sf - if(results[ i ] == results[ i - 1]){ - if(j > 2) - stop("Too low complexity to estimate k2n distribution") - else - j <- j + 1 - }else - j <- 1 - } - - #assign molecules number to unique barcode number: - k2n <- c() - for( i in 1:floor( max( results ) ) ) { - abs( results - i ) -> difference - - #if you want to know how many molecules underlie n unique barcodes - #ask k2n[n] - k2n[ i ] <- which( difference == min( difference ) ) - } - - #Assign +Inf for fragments which have saturated the barcodes - #(see correct_oversaturation function): - if( barcodes_saturated ) - k2n[max_observed + 1] <- Inf - - if(!missing(output_file)) { - write(k2n, file = output_file ) - } else - k2n -} - -# Read inputs -merged <- args[1] -unique_barcodes <- args[2] -output <- args[3] -paired_check <- as.logical(args[4]) - -k2n_calc( merged, unique_barcodes, output, paired = paired_check) diff -r fb76303acf4f -r 431aebd93843 rna_probing/normalize.R --- a/rna_probing/normalize.R Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,169 +0,0 @@ -##!/usr/bin/Rscript - -## Setup R error handling to go to stderr -options( show.error.messages = FALSE, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) - -# we need that to not crash galaxy with an UTF8 error on LC settings. -Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") - -suppressMessages(library('getopt')) - -#get options, using the spec as defined by the enclosed list. -#we read the options from the default: commandArgs(TRUE). -spec = matrix(c( - 'treated', 't', 1, "character", - 'control', 'c', 2, "character", - 'method', 'm', 1, "character", - 'fuComplexity', 'b', 2, "integer", - 'k2nTreated', 'k', 2, "character", - 'k2nControl', 'a', 2, "character", - 'cutoff', 'g', 1, "integer", - 'reference', 'r', 2, "character", - 'compdata', 'h', 2, "character", - 'dtcr', 'd', 0, "logical", - 'dtcrWindow', 'e', 2, "integer", - 'dtcrToZero', 'f', 2, "character", - 'slograt', 's', 0, "logical", - 'slogratWindow', 'p', 2, "integer", - 'depthCorrection', 'q', 2, "character", - 'pseudocount', 'l', 2, "integer", - 'swinsor', 'w', 0, "logical", - 'swinsorWindow', 'x', 2, "integer", - 'winsorLevel', 'y', 2, "double", - 'fixQuantile', 'z', 2, "character", - 'ntOffset', 'n', 2, "integer", - 'outputDir', 'o', 1, "character", - 'bedgraph', 'v', 2, "character", - 'bed', 'j', 2, "character", - 'genome', 'i', 2, "character", - 'trackName', 'u', 2, "character" -), byrow=TRUE, ncol=4); - -opt = getopt(spec); - -suppressMessages(require(RNAprobR)) - - -#Create output dir -dir.create(opt$outputDir, showWarnings = FALSE, recursive = TRUE) - - -if (opt$method=="counts"){ - treated_euc <- readsamples(opt$treated, euc=opt$method) -} else if (opt$method=="Fu") { - treated_euc <- readsamples(opt$treated, euc=opt$method, m = opt$fuComplexity) -} else { - treated_euc <- readsamples(opt$treated, euc=opt$method, k2n_files=opt$k2nTreated) -} - - -if ( !is.null(opt$reference)){ - comp_treated <- comp(treated_euc, cutoff=opt$cutoff, - fasta_file=opt$reference) -} else { - comp_treated <- comp(treated_euc, cutoff=opt$cutoff) -} - - -#If present, read control file -if ( !is.null(opt$control) && opt$control != 'None'){ - if (opt$method=="counts"){ - control_euc <- readsamples(opt$control, euc=opt$method) - } else if (opt$method=="Fu") { - control_euc <- readsamples(opt$control, euc=opt$method, m = opt$fuComplexity) - } else { - control_euc <- readsamples(opt$control, euc=opt$method, k2n_files=opt$k2nControl) - } - if ( !is.null(opt$reference)){ - comp_control <- comp(control_euc, cutoff=opt$cutoff, - fasta_file=opt$reference) - } else { - comp_control <- comp(control_euc, cutoff=opt$cutoff) - } -} - -#compdata -if ( !is.null(opt$compdata) ) { - - normalized <- compdata(comp_treated, nt_offset=opt$ntOffset) - colnames(mcols(normalized))[1:4] <- paste(colnames(mcols(normalized))[1:4], ".treated", sep="") - - if ( !is.null(opt$control) && opt$control != 'None') { - normalized <- compdata(comp_control, nt_offset=opt$ntOffset, add_to=normalized) - colnames(mcols(normalized))[6:9] <- c("TC.control", "TCR.control", "Cover.control", "PC.control") - } -} - -#dtcr -if ( !is.null(opt$dtcr) ) { - if ( exists("normalized")) { - normalized <- dtcr(comp_control, comp_treated, window_size=opt$dtcrWindow, - nt_offset=opt$ntOffset, bring_to_zero=opt$dtcrToZero, - add_to=normalized) - } - else { - normalized <- dtcr(comp_control, comp_treated, window_size=opt$dtcrWindow, - nt_offset=opt$ntOffset, bring_to_zero=opt$dtcrToZero) - } -} - -#slograt -if ( !is.null(opt$slograt) ) { - if ( exists("normalized")) { - normalized <- slograt(comp_control, comp_treated, window_size=opt$slogratWindow, - nt_offset=opt$ntOffset, depth_correction = opt$depthCorrection, - pseudocount=opt$pseudocount, add_to=normalized) - } - else { - normalized <- slograt(comp_control, comp_treated, window_size=opt$slogratWindow, - nt_offset=opt$ntOffset, depth_correction = opt$depthCorrection, - pseudocount=opt$pseudocount) - } -} - -#swinsor -if ( !is.null(opt$swinsor) ) { - if ( exists("normalized")) { - normalized <- swinsor(comp_treated, winsor_level = opt$winsorLevel, - window_size=opt$swinsorWindow, - only_top=opt$fixQuantile, nt_offset=opt$ntOffset, - add_to=normalized) - } - else { - normalized <- swinsor(comp_treated, winsor_level = opt$winsorLevel, - window_size=opt$swinsorWindow, - only_top=opt$fixQuantile, nt_offset=opt$ntOffset) - } -} - -#bedgraph output -if ( !is.null(opt$bedgraph)) { - if ( !is.null(opt$dtcr)){ - norm2bedgraph(normalized, bed_file = opt$bed, norm_method = "dtcr", - genome_build = opt$genome, - bedgraph_out_file = paste(opt$outputDir, "/dtcr", - sep=""), - track_name = opt$trackName, - track_description = opt$trackDesc) - } - if ( !is.null(opt$slograt)){ - norm2bedgraph(normalized, bed_file = opt$bed, norm_method = "slograt", - genome_build = opt$genome, - bedgraph_out_file = paste(opt$outputDir, "/slograt", - sep=""), - track_name = opt$trackName, - track_description = opt$trackDesc) - } - if ( !is.null(opt$swinsor)){ - norm2bedgraph(normalized, bed_file = opt$bed, norm_method = "swinsor", - genome_build = opt$genome, - bedgraph_out_file = paste(opt$outputDir, "/swinsor", - sep=""), - track_name = opt$trackName, - track_description = opt$trackDesc) - } -} - -output <- GR2norm_df(normalized) -write.table( output, paste(opt$outputDir, "/norm_df.txt", sep = ""), sep = "\t", - quote = F, row.names = F) diff -r fb76303acf4f -r 431aebd93843 rna_probing/normalize.xml --- a/rna_probing/normalize.xml Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,248 +0,0 @@ - - RNA probing signal - - R_3_1_1 - RNAprobR - RNAprobR - RNA_PROBING_SCRIPT_PATH - - - - normalize.R - - -t $treated - -c $control - - --method $euc_method.euc - - #if str($euc_method.euc) == "Fu": - --fuComplexity $euc_method.complexity - #else if str($euc_method.euc) == "HRF-Seq": - --k2nTreated $euc_method.k2n_treated - --k2nControl $euc_method.k2n_control - #end if - - --reference $fasta - - #if str($compdata) == 'yes': - --compdata - #end if - - #if str($dtcr.check) == 'yes': - --dtcr - --dtcrWindow $dtcr.wsize - --dtcrToZero $dtcr.zero - #end if - - #if str($slograt.check) == 'yes': - --slograt - --slogratWindow $slograt.wsize - --depthCorrection $slograt.depth_cor - --pseudocount $slograt.pseudocount - #end if - - #if str($swinsor.check) == 'yes': - --swinsor - --swinsorWindow $swinsor.wsize - --winsorLevel $swinsor.winsor_level - --fixQuantile $swinsor.only_top - #end if - - --ntOffset $nt_offset - --cutoff $cutoff - - #if str($bedgraph.check) == 'yes': - --bedgraph - --bed "$bedgraph.bed_file" - --genome "$bedgraph.genome" - --trackName "$bedgraph.track_name" - #end if - - -o 'output_dir' - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - bedgraph['check'] == 'yes' and dtcr['check'] == 'yes' - - - - - - - - - - - bedgraph['check'] == 'yes' and slograt['check'] == 'yes' - - - - - - - - - - - bedgraph['check'] == 'yes' and swinsor['check'] == 'yes' - - - - - - - - - - - - - - - -**What it does** - -Normalize tool performs termini based signal detection using summarized counts of sequenced reads (**Read Counts**) or sets of reads carrying the same barcode seqeuence (**Unique Barcodes**). The number of unique barcodes can be converted to Estimated Unique Counts (EUCs) by correcting for the existence of distinct barcodes of the same sequence with (Fu et al., 2011) or without (Kielpinski and Vinther, 2014) the assumption that all barcodes have the same probability of being captured. - ------- - -**Inputs** - -**Unique Barcodes** or **Read Counts** files produced by *Summarize Unique Barcodes* tool for treated and control samples. If deltaTCR of Smooth Log2 Ratio is desired a Control files are required. - -**k2n file(s)** is (are) required if "HRF-Seq" is selected as the "EUC" method. - -**Reference sequences** in FASTA format. Use the file that you produced the read alignment with. - ------- - -**EUC method** - - * **Counts** - Preserves the count of unique barcodes or read counts (depending on the input). It is the only suitable method for reading-in “Read Counts” file and an applicable method when the highest observed unique barcode count is lower than the square root of the count of all possible barcode combinations (Casbon et al., 2011). - - * **Fu** - Allows different barcodes to share the same sequence and assumes equal probability of ligating each barcode sequence (Fu et al., 2011). When selected the value of the random barcode complexity must be specified (e.g. barcode signature NWTRYSNNNN complexity equals 4×2×1×2×2×2×4×4×4×4 = 16384). - - * **HRF-Seq** - Similar to "Fu" but the probability of ligating different barcodes is estimated via observed frequencies of nucleotides at each barcode position (Kielpinski and Vinther, 2014). Requires k2n files for both treated and control samples. - ------- - -**Normalization methods** - -For a detailed description of the methods used read (publication pending) - ------- - -**Outputs** - -A tab seperated file with normalized signal values: - -====== ============================================================================================== -Column Description ------- ---------------------------------------------------------------------------------------------- - 1 Transcript identifier - 2 Position - 3 Nucleotide - 4- Normalized signal value followed by a column with a test significance value (where applicable) -====== ============================================================================================== - -. - -A BedGraph_ file that can be used for direct data display in the `UCSC Browser`_. - -.. _BedGraph: http://genome.ucsc.edu/goldenpath/help/bedgraph.html - -.. _UCSC Browser: http://genome.ucsc.edu/ - - - - - 10.1093/nar/gku167 - 10.1073/pnas.1017621108 - 10.1093/nar/gkr217 - - - diff -r fb76303acf4f -r 431aebd93843 rna_probing/plot_rna.R --- a/rna_probing/plot_rna.R Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,41 +0,0 @@ -#Setup R error handling to go to stderr -options( show.error.messages = FALSE, error = function () { cat( geterrmessage(), file=stderr() ); q( "no", 1, F ) } ) - -# we need that to not crash galaxy with an UTF8 error on German LC settings. -Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") - -suppressMessages(library('getopt')) - -#get options, using the spec as defined by the enclosed list. -#we read the options from the default: commandArgs(TRUE). -spec = matrix(c( - 'help' , 'h', 0, "logical", - 'input', 'i', 1, "character", - 'transcript', 't', 1, "character", - 'method', 'm', 2, "character", - 'cutoff', 'c', 2, "double", - 'type', 'p', 2, "character", - 'output', 'o', 1, "character" -), byrow=TRUE, ncol=4); - -opt = getopt(spec); - -suppressMessages(require(RNAprobR)) - -#Read and convert input to GRanges object -data <- read.table(opt$input, header = TRUE) -dataGR <- norm_df2GR(data) - -#Check if given transcript exists in input file -if ( ! opt$transcript %in% data$RNAid ) { stop("Transript not found. Check input file.") } - -pdf(opt$output) -for (method in strsplit(opt$method, ",")[[1]] ) { - - #Check if columns exists in data file - if (! method %in% colnames(data)) { next } - - plotRNA(dataGR, opt$transcript, method, stat_cutoff = opt$cutoff, type = opt$type, - main=paste(opt$transcript,": ", method, sep="")) -} -dev.off() diff -r fb76303acf4f -r 431aebd93843 rna_probing/plot_rna.xml --- a/rna_probing/plot_rna.xml Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,72 +0,0 @@ - - - - - R_3_1_1 - RNAprobR - RNAprobR - RNA_PROBING_SCRIPT_PATH - - - - plot_rna.R -i $input -t "$transcript" -m $norm_method -c $cutoff -p $plot_type -o $plot > /dev/null - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -**What it does** - -Produces plots for a given transcript in the tabular file produced by *Normalize* tool (one plot per selected method). If a selected normalizing method is not included in the input file it simply not be plotted. - ------- - -**Example** - -Input (head):: - - RNAid Pos nt dtcr dtcr.p - 16S_rRNA_E.coli 1 A 0 0.00787957002462928 - 16S_rRNA_E.coli 2 A 0.000606618229204106 2.87738710042044e-07 - 16S_rRNA_E.coli 3 A 0.00170457733442839 0.0242502062681708 - 16S_rRNA_E.coli 4 T 0.00171914631528914 0.504983784068465 - 16S_rRNA_E.coli 5 T 0.00111252808608504 5.26073688354678e-06 - 16S_rRNA_E.coli 6 G 0.00175507669063296 9.92896700324683e-05 - -Output (Significance: 1e-08, type: histogram) - -.. image:: http://people.binf.ku.dk/slm279/plot.png - - - diff -r fb76303acf4f -r 431aebd93843 rna_probing/preprocessing.sh --- a/rna_probing/preprocessing.sh Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,156 +0,0 @@ -#!/bin/bash - -#################################################################################################### -#Copyright (C) 2014 Lukasz Kielpinski, Nikos Sidiropoulos - -#This program is free software: you can redistribute it and/or modify it under the terms of the -#GNU General Public License as published by the Free Software Foundation, either version 3 of the -#License, or (at your option) any later version. - -#This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without -#even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the -#GNU General Public License for more details (http://www.gnu.org/licenses/). -#################################################################################################### - -#Preprocessing workflow - Read debarcoding and trimming. - -function print_help { -cat < -r -b -t -o -End-of-message -exit -} - -#defaults -output_dir="./output_dir" -read2="" -trim_length=0 - -#parse input -while getopts h1:2:b:t:o: myarg -do case "$myarg" in - h) print_help - exit ;; - 1) read1="$OPTARG" ;; #required - 2) read2="$OPTARG" ;; #optional - b) barcode="$OPTARG" ;; #optional - t) trim_length="$OPTARG" ;; #optional - o) output_dir="$OPTARG" ;; #optional - [?]) echo "ERROR: Unknown parameter" - print_help - exit 1 ;; - esac -done - -if [[ -z $read1 ]]; then - echo "ERROR: Read1 file required!" - print_help - exit 1 -fi - -# Create the output folder -mkdir -p $output_dir - -if [ -z "$barcode" ]; then - barcode='' -fi -BAR_LENGTH=`eval echo ${#barcode}` - -################################################################################################### -# Convert the Barcode signature to a regular exression. - -# Example: -# -# Barcode in the oligo used: NWTRYSNNNN -# Which means that each proper read must begin with: NNNN(C|G)(A|G)(C|T)A(A|T)N -# As regex: -# ^[ACGT][ACGT][ACGT][ACGT][CG][AG][CT][A][AT][ACGT] -# Reverse complement the barcode sequence and create a regular expression. - -REV_COMPLEMENT=$(perl -0777ne's/\n //g; tr/ATGCatgcNnYyRrKkMmBbVvDdHh/TACGtacgNnRrYyMmKkVvBbHhDd/; print scalar reverse $_;' <(echo $barcode)) - -REG_EXP=$(sed 's/A/[A]/g;s/C/[C]/g;s/G/[G]/g;s/T/[T]/g;s/R/[AG]/g;s/Y/[CT]/g;s/S/[GC]/g;s/W/[AT]/g;s/K/[GT]/g;s/M/[AC]/g;s/B/[CGT]/g;s/D/[AGT]/g;s/H/[ACT]/g;s/V/[ACG]/g;s/N/[AGCTN]/g;' <<< $REV_COMPLEMENT) - -################################################################################################### -#Remove reads that do not start with the signature (Read1) - -#First awk removes them, second awk removes corresponding quality strings. -#Remove last N nt - may be derived from the random primers. - -awk '{if(NR%4==2){if(/^'"$REG_EXP"'/){print}else{print ""}}else{print}}' $read1 | - -awk 'BEGIN{trim_flag=0; trimming_stats=0; all_processed=0} -{ - if(NR%4==1){print; all_processed++} - if(NR%4==2){if(length($1)==0){trim_flag=1;trimming_stats++}else{trim_flag=0};print} - if(NR%4==3){print} - if(NR%4==0){if(trim_flag==1){print ""}else{print $0}} -}END{print(trimming_stats, all_processed) > "trimming_stats.error"}' | - -awk -v len="${trim_length}" '{if(NR%2==0){print(substr($1,0,length($1)-len))}else{print}}' > R1.fastq & - -wait - -mv trimming_stats.error $output_dir/trimming_stats.txt - -################################################################################################### -#Trim primers and possible random barcode - -if [ -z "$read2" ]; then - #single-end - - #Extract the barcode sequence from the first read: - awk -v len="${BAR_LENGTH}" '{if(NR%2==0){print(substr($0,len+1,length($0)))}else{print($0)}}' R1.fastq | awk '{print($1)}' > $output_dir/read1.fastq & - - awk -v len="${BAR_LENGTH}" '{if(NR%4==1){print($1)}else{if(NR%4==2){print(substr($0,0,len))}}}' R1.fastq | paste - - > $output_dir/barcodes.txt & - - wait - - #Remove temp files - rm R1.fastq - -else - #paired-end - - #Trim primers (Read2) - awk -v len1="${trim_length}" -v len2="${BAR_LENGTH}" '{if(NR%2==0){print(substr($0,len1+1,(length($0)-len1-len2)))}else{print($0)}}' $read2 > R2.fastq - - ## Fix pairs in Read1 and Read2 fastq files - #Sort - awk '{print($1)}' R1.fastq | paste - - - - | awk '(NF==4){print $0}' | sort -S1G -k1,1 > R1_collapsed.fastq & - awk '{print($1)}' R2.fastq | paste - - - - | awk '(NF==4){print $0}' | sort -S1G -k1,1 > R2_collapsed.fastq & - - wait - - join -1 1 -2 1 R1_collapsed.fastq R2_collapsed.fastq > joined.fastq - - awk 'BEGIN{OFS=""}{print($1"\n"$2"\n"$3"\n"$4)}' joined.fastq > R1_sorted.fastq & - awk 'BEGIN{OFS=""}{print($1"\n"$5"\n"$6"\n"$7)}' joined.fastq > $output_dir/read2.fastq & - - wait - - rm joined.fastq R1_collapsed.fastq R2_collapsed.fastq R1.fastq R2.fastq & - - ######################################################################## - #Extract the barcode sequence from the first read: - awk -v len="${BAR_LENGTH}" '{if(NR%2==0){print(substr($0,len+1,length($0)))}else{print($0)}}' R1_sorted.fastq | awk '{print($1)}' > $output_dir/read1.fastq & - - awk -v len="${BAR_LENGTH}" '{if(NR%4==1){print($1)}else{if(NR%4==2){print(substr($0,0,len))}}}' R1_sorted.fastq | paste - - > $output_dir/barcodes.txt & - - wait - - #Remove temp files - rm R1_sorted.fastq - -fi diff -r fb76303acf4f -r 431aebd93843 rna_probing/preprocessing.xml --- a/rna_probing/preprocessing.xml Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,136 +0,0 @@ - - RNA probing data - - - gnu_awk - RNA_RPOBING_SCRIPT_PATH - - - - preprocessing.sh - - ## check if paired-end - #if str( $library.type ) == "paired" - -2 $library.input2 - #end if - - ## Inputs - -1 $library.input1 - - ## Barcode sequence - -b '$library.barcode_seq' - - ## Trimming length - -t $trim - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - library['type'] == "paired" - - - - - - - - - - - - - - - - - - - -**What it does** - -*Preprocessing* tool removes and saves the random barcodes sequences, if they were ligated to 3’ ends of cDNA, in a separate dataset to be used in downstream analysis. Additionally to debarcoding, it trims 1) the 5’ end of the second-in-pair reads to remove the reverse transcription primer derived sequence and 2) 3’ end of both reads to remove possible random barcode incorporation in the second-in-pair read and random primer in first-in-pair read. - ------- - -**Examples** - -Sample input files (quality scores omited):: - - * Read1 - @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 1:N:0:ATCACG - TTCGCACAACATNATGGAGGCTTCACGGTACAGAACGAGGCCAGCAAATACCAAGTCTCAGTGAACAAATACAAAGGGACGGCTGGCAACGCCCTCAT - @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 1:N:0:ATCACG - ACCCCGCATCAAATTGGGAACTACTTCCAGCAGTTGTTAGACTTGGGCTCTGGCAGCCCCTTGGAGTGGAGGGACTTGCAGCCCTTCTTATCAGGTCT - - * Read2 - @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 2:N:0:ATCACG - CACAAATCTGCCGTTTGGATTGGCTGCATGGCATCTGTTATACCACCAGCCACCACCATCTTCTTTGGAGCACTGTTTTCTTGGATCCGTAGTTACCC - @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 2:N:0:ATCACG - GTTGGGGGTGTGGGGAAAAAAATAAAAATCGTGAGAAGTTTTAAGACTATGTCACAAAAATGGCTTTAATTATACCATCAAACAGAAACCACCAATTG - -Run 1 - Barcode Sequence = '', Trimming length = 10:: - - * Read1 - @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 - TTCGCACAACATNATGGAGGCTTCACGGTACAGAACGAGGCCAGCAAATACCAAGTCTCAGTGAACAAATACAAAGGGACGGCTGGCA - @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 - ACCCCGCATCAAATTGGGAACTACTTCCAGCAGTTGTTAGACTTGGGCTCTGGCAGCCCCTTGGAGTGGAGGGACTTGCAGCCCTTCT - - * Read2 - @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 - CCGTTTGGATTGGCTGCATGGCATCTGTTATACCACCAGCCACCACCATCTTCTTTGGAGCACTGTTTTCTTGGATCCGTAGTTACCC - @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 - TGGGGAAAAAAATAAAAATCGTGAGAAGTTTTAAGACTATGTCACAAAAATGGCTTTAATTATACCATCAAACAGAAACCACCAATTG - -Run 2 - Barcode Sequence = 'NNNNNNN', Trimming length = 10:: - - * Read1 - @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 - AACATNATGGAGGCTTCACGGTACAGAACGAGGCCAGCAAATACCAAGTCTCAGTGAACAAATACAAAGGGACGGCTGGCA - @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 - ATCAAATTGGGAACTACTTCCAGCAGTTGTTAGACTTGGGCTCTGGCAGCCCCTTGGAGTGGAGGGACTTGCAGCCCTTCT - - * Read2 - @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 - CCGTTTGGATTGGCTGCATGGCATCTGTTATACCACCAGCCACCACCATCTTCTTTGGAGCACTGTTTTCTTGGATCCGTA - @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 - TGGGGAAAAAAATAAAAATCGTGAGAAGTTTTAAGACTATGTCACAAAAATGGCTTTAATTATACCATCAAACAGAAACCA - - * Barcodes - - @DJG83KN1:255:C3U57ACXX:3:1101:1215:2296 TTCGCAC - @DJG83KN1:255:C3U57ACXX:3:1101:1142:2461 ACCCCGC - - - - - 10.1093/nar/gku167 - - - diff -r fb76303acf4f -r 431aebd93843 rna_probing/summarize_unique_barcodes.sh --- a/rna_probing/summarize_unique_barcodes.sh Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,231 +0,0 @@ -#!/bin/bash - -#################################################################################################### -#Copyright (C) 2014 Lukasz Kielpinski, Nikos Sidiropoulos - -#This program is free software: you can redistribute it and/or modify it under the terms of the -#GNU General Public License as published by the Free Software Foundation, either version 3 of the -#License, or (at your option) any later version. - -#This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without -#even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the -#GNU General Public License for more details (http://www.gnu.org/licenses/). -#################################################################################################### - -function print_help { -cat < -b -p -t -k -r -End-of-message -exit -} - -#defaults -output_dir="output_dir" -trim_flag="False" - -#parse input -while getopts hf:b::p:o:ktr: myarg -do case "$myarg" in - h) print_help - exit ;; - f) bamfile="$OPTARG" ;; #required - b) barcodes="$OPTARG" ;; #optional - t) trim_flag="True" ;; - k) k2n="True" ;; - p) priming_pos="$OPTARG" ;; - o) output_dir="$OPTARG" ;; - r) R_SCRIPT_PATH="$OPTARG" ;; #required - [?]) echo "ERROR: Unknown parameter" - print_help - exit 1 ;; - esac -done - -###### Sanity checks ###### -if [ -z $bamfile ]; then - echo "Error: Aligned reads file is missing!" - print_help - exit 1 -fi - -if [ "$barcodes" == "None" ] && [ "$k2n" == "True" ]; then - echo "Error: k2n file cannot be produced without a barcode file!" - exit 1 -fi - - -mkdir -p $output_dir - -#Check if bamfile contains single or paired-end reads -samtools view -f 0x1 $bamfile | head -n 1 > paired - -if [ -s paired ]; then - #paired-end - samtools view $bamfile | awk 'BEGIN{OFS="\t"}{if(substr($0,1,1)!="@"){print}}' - | awk -v out="${output_dir}/trimming_stats.txt" -v flag="${trim_flag}" 'BEGIN{OFS="\t";counter[0]=0;counter[1]=0;counter[2]=0;counter[3]=0;counter[4]=0} - function abs(value){return(value<0?-value:value)} - function return_offset(local_offset){print($1, $3, $4+local_offset, $4+abs($9)-1);counter[local_offset]++} - ($2 != 99) {next}; - (flag == "False") {return_offset(0);next}; - (/[\s\t]MD:Z:/ && !/MD:Z:([012][ACGT])/) {return_offset(0);next}; - (/[\s\t]MD:Z:0[ACGT]/ && !/MD:Z:0[ACGT][01][ACGT]/ && substr($10,1,1)=="N") {return_offset(0);next}; - (/[\s\t]MD:Z:0[ACGT]/ && !/MD:Z:0[ACGT][01][ACGT]/) {return_offset(1);next}; - - (/[\s\t]MD:Z:1[ACGT]/ && !/MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(0);next}; - (/[\s\t]MD:Z:1[ACGT]/ && !/MD:Z:1[ACGT]0[ACGT]/) {return_offset(2);next}; - - (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,1,2)=="NN") {return_offset(0);next}; - (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,1,1)=="N") {return_offset(2);next}; - (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(1);next}; - (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(2);next}; - - (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,2)=="NN") {return_offset(0);next}; - (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(3);next}; - (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,3,1)=="N") {return_offset(2);next}; - (/[\s\t]MD:Z:1[ACGT]0[ACGT]/) {return_offset(3);next}; - - (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,3,1)=="N" && substr($10,1,1)=="N") {return_offset(0);next}; - (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,3,1)=="N") {return_offset(1);next}; - (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,1,1)=="N") {return_offset(3);next}; - (/MD:Z:0[ACGT]1[ACGT]/) {return_offset(3);next}; - - (/MD:Z:2[ACGT]/ && substr($10,3,1)=="N") {return_offset(0);next}; - (/MD:Z:2[ACGT]/) {return_offset(3);next}; - - (substr($10,3,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(3);next}; - (substr($10,2,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(2);next}; - (substr($10,1,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(1);next}; - - {return_offset(0);counter[4]++} - END{print("No trimming:",counter[0],", out of which not recognized MD field for:",counter[4],"; 1 nt trimmed:", counter[1],"; 2 nt trimmed:", counter[2],"; 3 nt trimmed:",counter[3]) > out}' | sort -S1G -k1,1 | gzip > positions_temp_sorted.gz - -else - #single-end - samtools view $bamfile | awk 'BEGIN{OFS="\t"}{if(substr($0,1,1)!="@"){print}}' - | awk -v out="${output_dir}/trimming_stats.txt" -v flag="${trim_flag}" 'BEGIN{OFS="\t";counter[0]=0;counter[1]=0;counter[2]=0;counter[3]=0;counter[4]=0} - function abs(value){return(value<0?-value:value)} - function return_offset(local_offset){print($1, $3, $4+local_offset, $4+abs($9)-1);counter[local_offset]++} - ($2 != 0) {next}; - (flag == "False") {return_offset(0);next}; - (/[\s\t]MD:Z:/ && !/MD:Z:([012][ACGT])/) {return_offset(0);next}; - (/[\s\t]MD:Z:0[ACGT]/ && !/MD:Z:0[ACGT][01][ACGT]/ && substr($10,1,1)=="N") {return_offset(0);next}; - (/[\s\t]MD:Z:0[ACGT]/ && !/MD:Z:0[ACGT][01][ACGT]/) {return_offset(1);next}; - - (/[\s\t]MD:Z:1[ACGT]/ && !/MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(0);next}; - (/[\s\t]MD:Z:1[ACGT]/ && !/MD:Z:1[ACGT]0[ACGT]/) {return_offset(2);next}; - - (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,1,2)=="NN") {return_offset(0);next}; - (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,1,1)=="N") {return_offset(2);next}; - (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(1);next}; - (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(2);next}; - - (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,2)=="NN") {return_offset(0);next}; - (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(3);next}; - (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,3,1)=="N") {return_offset(2);next}; - (/[\s\t]MD:Z:1[ACGT]0[ACGT]/) {return_offset(3);next}; - - (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,3,1)=="N" && substr($10,1,1)=="N") {return_offset(0);next}; - (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,3,1)=="N") {return_offset(1);next}; - (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,1,1)=="N") {return_offset(3);next}; - (/MD:Z:0[ACGT]1[ACGT]/) {return_offset(3);next}; - - (/MD:Z:2[ACGT]/ && substr($10,3,1)=="N") {return_offset(0);next}; - (/MD:Z:2[ACGT]/) {return_offset(3);next}; - - (substr($10,3,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(3);next}; - (substr($10,2,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(2);next}; - (substr($10,1,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(1);next}; - - {return_offset(0);counter[4]++} - END{print("No trimming:",counter[0],", out of which not recognized MD field for:",counter[4],"; 1 nt trimmed:", counter[1],"; 2 nt trimmed:", counter[2],"; 3 nt trimmed:",counter[3]) > out}' | sort -S1G -k1,1 | gzip > positions_temp_sorted.gz - -fi - -#Computing barcode length (Use the first line and compute the string length of the second column) -if [ "$barcodes" != "None" ]; then - - TMP=$(for line in `cut -f 2 $barcodes`; do if [ ! -z "$line" ]; then echo $line; break; fi; done) - BAR_LEN=`echo ${#TMP}` - - #Remove "@" from barcodes and sort them - sed 's/^.//' $barcodes | sort -k1,1 -S1G | gzip > barcodes_temp_sorted.gz - - #Merge poistions and barcodes - join -1 1 <(zcat positions_temp_sorted.gz) <(zcat barcodes_temp_sorted.gz) | cut -f 2,3,4,5 -d " " | awk '{if($4 !~ /N/){print}}' | awk -v bar_len="${BAR_LEN}" '{if(length($4)==bar_len){print}}' | gzip > merged_temp.gz - - rm barcodes_temp_sorted.gz - -else - zcat positions_temp_sorted.gz | cut -f 2,3,4 | gzip > merged_temp.gz -fi - -#If the experiment is single-end set NA values to the priming column. -if [ ! -s paired ]; then - zcat merged_temp.gz | awk '{print $1, $2, "NA", $4, $5}' - > merged_temp2 - gzip -c merged_temp2 > merged_temp.gz - rm merged_temp2 -fi - -#Fix priming position -if [ ! -z $priming_pos ]; then - zcat merged_temp.gz | awk -v pos="${priming_pos}" '{print $1, $2, pos, $4, $5}' - > merged_temp2 - gzip -c merged_temp2 > merged_temp.gz - rm merged_temp2 -fi - -#File summary.txt columns: RNA_ID, Start, End, barcode sequence, sequenced_count[=number of sequenced fragments fulfilling previous requiremnts] - -zcat merged_temp.gz | awk '{barcode[$1][$2][$3][$4]++}END{ -for(RNA in barcode){ -for(start_position in barcode[RNA]){ -for(end_position in barcode[RNA][start_position]){ -for(barseq in barcode[RNA][start_position][end_position]){print RNA,start_position,end_position,barseq,barcode[RNA][start_position][end_position][barseq]}}}}}' > $output_dir/summary.txt - -#File unique_barcodes.txt columns: RNA_ID, Start, End, number of unique barcodes observed for this fragment. - -awk '{barcode[$1][$2][$3]++}END{ -for(RNA in barcode){ -for(start_position in barcode[RNA]){ -for(end_position in barcode[RNA][start_position]){print RNA "\t" start_position "\t" end_position "\t" barcode[RNA][start_position][end_position]}}}}' $output_dir/summary.txt > $output_dir/unique_barcodes.txt & - -#File read_counts.txt colums: RNA_ID, Start, End, sequenced_count - -zcat merged_temp.gz | awk '{barcode[$1][$2][$3]++}END{ -for(RNA in barcode){ -for(start_position in barcode[RNA]){ -for(end_position in barcode[RNA][start_position]){print RNA "\t" start_position "\t" end_position "\t" barcode[RNA][start_position][end_position]}}}}' > $output_dir/read_counts.txt & - -wait - -#Print the maximum observed barcodes value. Usefull to assess the necessity of producing the k2n file. -if [ "$barcodes" != "None" ]; then - - cut -f 4 $output_dir/unique_barcodes.txt | sort -S1G -rn > sorted_bars - max_observed_barcodes=`head -n 1 sorted_bars` - - echo "Maximum observed Barcodes = ${max_observed_barcodes}" - rm sorted_bars -fi - -#Produce k2n file -if [ "$k2n" == "True" ]; then - if [ ! -s paired ]; then - Rscript $R_SCRIPT_PATH/k2n.R merged_temp.gz $output_dir/unique_barcodes.txt $output_dir/k2n.txt FALSE - else - Rscript $R_SCRIPT_PATH/k2n.R merged_temp.gz $output_dir/unique_barcodes.txt $output_dir/k2n.txt TRUE - fi -fi - -#Remove temp files -rm merged_temp.gz positions_temp_sorted.gz paired diff -r fb76303acf4f -r 431aebd93843 rna_probing/summarize_unique_barcodes.xml --- a/rna_probing/summarize_unique_barcodes.xml Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,139 +0,0 @@ - - - - - gnu_awk - samtools - R_3_1_1 - RNAprobR - RNAprobR - RNA_PROBING_SCRIPT_PATH - - - - summarize_unique_barcodes.sh - - ## Inputs - -f $input1 -b $input2 - - ## - - #if str( $k2n ) == 'True': - -k - #end if - - #if str( $priming.flag ) == 'True': - -p $priming.position - #end if - - #if str( $trimming ) == 'True': - -t - #end if - - -r \$RNA_PROBING_SCRIPT_PATH - - - - - - - - - - - - - - - - - - - - - - - - - - - trimming is True - - - input2 != None - - - - k2n is True - - - - - - - - - - - - - - - - -**What it does** - -*Summarize Unique Barcodes* counts the number of unique random barcodes and reads associated with each sequenced fragment. A fragment is understood as 1) a pair of Reverse Transcriptase (RT) termination site and RT priming site given paired end sequencing, or 2) an RT termination site in a single end sequencing. For non-barcoded sequencing it only counts the reads matching each fragment. - ------- - -**Inputs** - -*Summarize Unique Barcodes* requires a file containing the Aligned Reads (required) in BAM_ format and tabular file with the Barcodes (optional) produced by the *Preprocessing* tool of the *RNA probing* suite. - -.. _BAM: http://samtools.github.io/hts-specs/SAMv1.pdf - -------- - -**Parameters** - -**Produce k2n file** - A file that contains a sequence of numbers where the n-th element informs how many unique cDNA molecules gives rise to observing n unique barcodes in a given sample. Required for calculating Estimated Unique Counts (EUCs) in tool *Normalize*. - -**Trim untemplated nucleotides** - Untemplated nucleotides can be added to cDNA 3’ ends via terminal transferase activity of reverse transcriptase which offset the location of the read-end mapping and lead to erroneous assignment of reactivity information to nucleotides upstream of those which has reacted (Schmidt and Mueller, 1999, Talkish et al., 2014). Setting this parameter on will remove those nucleotides. - -Recommended for methods based on detecting reverse transcription termination sites (e.g. DMS-Seq, HRF-Seq or SHAPE-Seq), and not for methods based on ligating the linker directly to RNA (e.g. PARS or FragSeq). - -**Set priming position** - Applicable when the priming site is fixed. - ------- - -**Outputs** - -**Unique Barcodes** (if a Barcode file is given) is a tabular file with 4 columns. - -====== ========================================================== -Column Description ------- ---------------------------------------------------------- - 1 Transcript identifier - 2 RT termination site (start) - 3 RT priming site (end) - 4 Count of unique barcodes associated with fragments matching the first three columns -====== ========================================================== - -. - -**Read Counts** is similar to Unique Barcodes but the fourth column is a count of reads matching first three columns. - -**k2n file** as described above. - -**Trimming Stats** reports statistics of trimming untemplated nucleotides from read ends. - - - - - 10.1093/nar/gku167 - 10.1093/nar/27.21.e31-i - 10.1261/rna.042218.113 - - - diff -r fb76303acf4f -r 431aebd93843 rna_probing/test-data/RNase_P.fa --- a/rna_probing/test-data/RNase_P.fa Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,4 +0,0 @@ ->RNase_P -GGGGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATAT -GGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCG -ATCCGCTTCGGCGGATCCAAATCGGGCTTCGGTCCGGTTCAGATCGGAAGAGCACACGTCT diff -r fb76303acf4f -r 431aebd93843 rna_probing/test-data/aligned.bam Binary file rna_probing/test-data/aligned.bam has changed diff -r fb76303acf4f -r 431aebd93843 rna_probing/test-data/barcodes.txt --- a/rna_probing/test-data/barcodes.txt Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,671 +0,0 @@ -@HWI-ST575:165:C1P5BACXX:1:1101:1666:1132 TCCATCC -@HWI-ST575:165:C1P5BACXX:1:1101:2239:1101 CAACCCC -@HWI-ST575:165:C1P5BACXX:1:1101:2455:1083 CCACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:3207:1238 TGCAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:3405:1152 TCAATAC -@HWI-ST575:165:C1P5BACXX:1:1101:3512:1179 TTTCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:4473:1182 GGCAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:5162:1092 CTTCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:6810:1177 CGATCAT -@HWI-ST575:165:C1P5BACXX:1:1101:8268:1157 CAAACGG -@HWI-ST575:165:C1P5BACXX:1:1101:8740:1185 GTATGAC -@HWI-ST575:165:C1P5BACXX:1:1101:12414:1099 ATCGGGC -@HWI-ST575:165:C1P5BACXX:1:1101:14053:1112 TATTCTC -@HWI-ST575:165:C1P5BACXX:1:1101:14219:1152 TCGTAAC -@HWI-ST575:165:C1P5BACXX:1:1101:15012:1211 GCCCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:15774:1231 ATTACAC -@HWI-ST575:165:C1P5BACXX:1:1101:17047:1127 AATGTTC -@HWI-ST575:165:C1P5BACXX:1:1101:18461:1188 CGATCAC -@HWI-ST575:165:C1P5BACXX:1:1101:19911:1138 TACCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:1311:1500 ACTAGTC -@HWI-ST575:165:C1P5BACXX:1:1101:2110:1396 CCCCCCT -@HWI-ST575:165:C1P5BACXX:1:1101:2970:1430 AGGCGAC -@HWI-ST575:165:C1P5BACXX:1:1101:4079:1444 CTGGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:5495:1254 TCGAACT -@HWI-ST575:165:C1P5BACXX:1:1101:5684:1314 TAATCCT -@HWI-ST575:165:C1P5BACXX:1:1101:6002:1450 GTTGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:6163:1455 TCAATGG -@HWI-ST575:165:C1P5BACXX:1:1101:6370:1342 CAGGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:7500:1303 ACTAAAA -@HWI-ST575:165:C1P5BACXX:1:1101:8186:1308 ATCCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:10049:1341 GATCTGC -@HWI-ST575:165:C1P5BACXX:1:1101:10737:1349 AAGCGAC -@HWI-ST575:165:C1P5BACXX:1:1101:11993:1308 CTGACCC -@HWI-ST575:165:C1P5BACXX:1:1101:12260:1420 TCGGTCG -@HWI-ST575:165:C1P5BACXX:1:1101:13041:1365 CAACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:13915:1290 TCACCCT -@HWI-ST575:165:C1P5BACXX:1:1101:13944:1432 GCAGCTC -@HWI-ST575:165:C1P5BACXX:1:1101:14362:1371 CATCGCA -@HWI-ST575:165:C1P5BACXX:1:1101:14865:1283 CATCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:15341:1459 AACCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:15856:1385 CGATCAC -@HWI-ST575:165:C1P5BACXX:1:1101:16168:1446 CCTATAC -@HWI-ST575:165:C1P5BACXX:1:1101:16274:1448 GACATAC -@HWI-ST575:165:C1P5BACXX:1:1101:17532:1255 GTCAGTC -@HWI-ST575:165:C1P5BACXX:1:1101:19852:1368 ACTCATG -@HWI-ST575:165:C1P5BACXX:1:1101:20338:1414 AACTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:1782:1682 ACTGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:2733:1718 TGCAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:2796:1586 TCACACT -@HWI-ST575:165:C1P5BACXX:1:1101:3075:1523 ACTAGTC -@HWI-ST575:165:C1P5BACXX:1:1101:4251:1515 AATACAC -@HWI-ST575:165:C1P5BACXX:1:1101:4481:1718 CATCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:4978:1615 GACGGCC -@HWI-ST575:165:C1P5BACXX:1:1101:6935:1687 AAACCAC -@HWI-ST575:165:C1P5BACXX:1:1101:7393:1637 TGCAGGA -@HWI-ST575:165:C1P5BACXX:1:1101:7744:1585 ACCTTTC -@HWI-ST575:165:C1P5BACXX:1:1101:7646:1624 CTTGTGT -@HWI-ST575:165:C1P5BACXX:1:1101:8080:1642 TTTGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:8452:1710 TCTAGTG -@HWI-ST575:165:C1P5BACXX:1:1101:8575:1513 GAGGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:8610:1649 TCTTACC -@HWI-ST575:165:C1P5BACXX:1:1101:9515:1701 TCCGCCC -@HWI-ST575:165:C1P5BACXX:1:1101:9782:1735 TTACCAC -@HWI-ST575:165:C1P5BACXX:1:1101:10892:1546 CCATTCT -@HWI-ST575:165:C1P5BACXX:1:1101:10875:1631 ATTTGGT -@HWI-ST575:165:C1P5BACXX:1:1101:11536:1555 TTGATGC -@HWI-ST575:165:C1P5BACXX:1:1101:11540:1624 TGGCCGC -@HWI-ST575:165:C1P5BACXX:1:1101:12292:1617 GGAAGTC -@HWI-ST575:165:C1P5BACXX:1:1101:13423:1569 CATCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:13576:1612 CACGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:14491:1526 TGACCAT -@HWI-ST575:165:C1P5BACXX:1:1101:15109:1589 CACTCCT -@HWI-ST575:165:C1P5BACXX:1:1101:16270:1554 TTGATGC -@HWI-ST575:165:C1P5BACXX:1:1101:17059:1656 GGACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:17823:1711 TCCTAAC -@HWI-ST575:165:C1P5BACXX:1:1101:18750:1719 CATATAC -@HWI-ST575:165:C1P5BACXX:1:1101:19397:1535 ATACTAC -@HWI-ST575:165:C1P5BACXX:1:1101:19270:1553 CTGCCAA -@HWI-ST575:165:C1P5BACXX:1:1101:20160:1653 ACACGAC -@HWI-ST575:165:C1P5BACXX:1:1101:20348:1677 GCATCAC -@HWI-ST575:165:C1P5BACXX:1:1101:1152:1999 TCCTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:1575:1782 CCCAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:2146:1765 TAATGAC -@HWI-ST575:165:C1P5BACXX:1:1101:2817:1860 CATTGAA -@HWI-ST575:165:C1P5BACXX:1:1101:4121:1770 GCCTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:4015:1808 TTCGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:4671:1789 TCGACAC -@HWI-ST575:165:C1P5BACXX:1:1101:5190:1986 TCGAGAC -@HWI-ST575:165:C1P5BACXX:1:1101:7320:1866 AAACTAC -@HWI-ST575:165:C1P5BACXX:1:1101:7508:1798 GCTAGTT -@HWI-ST575:165:C1P5BACXX:1:1101:9647:1841 TTCGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:11078:1760 CGCACTC -@HWI-ST575:165:C1P5BACXX:1:1101:12091:1910 TTACGCA -@HWI-ST575:165:C1P5BACXX:1:1101:13182:1885 ACTGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:13475:1836 AATCCAC -@HWI-ST575:165:C1P5BACXX:1:1101:14275:1813 AGCTGAC -@HWI-ST575:165:C1P5BACXX:1:1101:14914:1754 GCTGTCA -@HWI-ST575:165:C1P5BACXX:1:1101:15826:1814 CAGACCT -@HWI-ST575:165:C1P5BACXX:1:1101:16316:1995 GGGATTC -@HWI-ST575:165:C1P5BACXX:1:1101:17677:1880 CCAAACC -@HWI-ST575:165:C1P5BACXX:1:1101:18640:1881 AACGTTC -@HWI-ST575:165:C1P5BACXX:1:1101:18890:1897 AGGCAGC -@HWI-ST575:165:C1P5BACXX:1:1101:1102:2244 GCCAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:2310:2103 GTCTACC -@HWI-ST575:165:C1P5BACXX:1:1101:3289:2146 TACTATT -@HWI-ST575:165:C1P5BACXX:1:1101:4895:2080 TACGAGC -@HWI-ST575:165:C1P5BACXX:1:1101:4932:2108 TATCAAG -@HWI-ST575:165:C1P5BACXX:1:1101:5806:2073 TCCCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:6214:2065 GCAAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:6143:2137 TCCACAC -@HWI-ST575:165:C1P5BACXX:1:1101:6241:2199 CGGTCAC -@HWI-ST575:165:C1P5BACXX:1:1101:7894:2030 ATTGTGC -@HWI-ST575:165:C1P5BACXX:1:1101:8072:2245 CCACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:8299:2087 ATACCCC -@HWI-ST575:165:C1P5BACXX:1:1101:8471:2183 ACACATT -@HWI-ST575:165:C1P5BACXX:1:1101:9051:2011 CGGTGAC -@HWI-ST575:165:C1P5BACXX:1:1101:11298:2099 ATTACCC -@HWI-ST575:165:C1P5BACXX:1:1101:11886:2227 AGGTCCC -@HWI-ST575:165:C1P5BACXX:1:1101:12469:2156 CCCGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:13307:2211 GCGGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:13967:2112 CACCATT -@HWI-ST575:165:C1P5BACXX:1:1101:14357:2064 AACCAAA -@HWI-ST575:165:C1P5BACXX:1:1101:14498:2106 TGACTAC -@HWI-ST575:165:C1P5BACXX:1:1101:14632:2166 GGGTCTC -@HWI-ST575:165:C1P5BACXX:1:1101:14873:2011 CCTTAAC -@HWI-ST575:165:C1P5BACXX:1:1101:15054:2152 GCCGGCC -@HWI-ST575:165:C1P5BACXX:1:1101:15855:2031 TCACCAA -@HWI-ST575:165:C1P5BACXX:1:1101:15913:2171 CACCATG -@HWI-ST575:165:C1P5BACXX:1:1101:16436:2126 CACCACC -@HWI-ST575:165:C1P5BACXX:1:1101:16535:2143 CAGGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:16829:2249 CACCATC -@HWI-ST575:165:C1P5BACXX:1:1101:17455:2003 TGGGTCC -@HWI-ST575:165:C1P5BACXX:1:1101:17878:2158 TTCAGAC -@HWI-ST575:165:C1P5BACXX:1:1101:18041:2200 CAGAACT -@HWI-ST575:165:C1P5BACXX:1:1101:2284:2369 CAGACAC -@HWI-ST575:165:C1P5BACXX:1:1101:2836:2439 TCTCTGC -@HWI-ST575:165:C1P5BACXX:1:1101:4292:2260 GACACTT -@HWI-ST575:165:C1P5BACXX:1:1101:4629:2330 TAATCGT -@HWI-ST575:165:C1P5BACXX:1:1101:4537:2454 TGGCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:5316:2437 GTTCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:5705:2286 TAAACAC -@HWI-ST575:165:C1P5BACXX:1:1101:6181:2354 TGCCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:6640:2254 GCATCCG -@HWI-ST575:165:C1P5BACXX:1:1101:6520:2397 ACCCCAC -@HWI-ST575:165:C1P5BACXX:1:1101:7019:2438 GATCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:8048:2260 TATTCTC -@HWI-ST575:165:C1P5BACXX:1:1101:8522:2464 GCCCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:8845:2462 GCGGCGG -@HWI-ST575:165:C1P5BACXX:1:1101:9068:2302 ACAACAC -@HWI-ST575:165:C1P5BACXX:1:1101:11110:2418 ATGACTC -@HWI-ST575:165:C1P5BACXX:1:1101:11986:2397 AATCCCT -@HWI-ST575:165:C1P5BACXX:1:1101:12227:2444 TATGACC -@HWI-ST575:165:C1P5BACXX:1:1101:12315:2285 GATGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:12492:2350 ACCAGAT -@HWI-ST575:165:C1P5BACXX:1:1101:13583:2475 CTCAAGC -@HWI-ST575:165:C1P5BACXX:1:1101:14469:2354 ACAAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:14989:2278 GCCATTC -@HWI-ST575:165:C1P5BACXX:1:1101:15188:2338 ACCTGAC -@HWI-ST575:165:C1P5BACXX:1:1101:15331:2257 CACACTT -@HWI-ST575:165:C1P5BACXX:1:1101:15849:2368 ACCGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:16152:2410 TAGCCAC -@HWI-ST575:165:C1P5BACXX:1:1101:16935:2365 GCTCTCC -@HWI-ST575:165:C1P5BACXX:1:1101:17289:2412 CGACCTC -@HWI-ST575:165:C1P5BACXX:1:1101:18384:2478 ACGGACT -@HWI-ST575:165:C1P5BACXX:1:1101:19068:2414 GTGCTAT -@HWI-ST575:165:C1P5BACXX:1:1101:19461:2478 TACTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:19502:2351 GCCAATC -@HWI-ST575:165:C1P5BACXX:1:1101:19741:2372 GCTGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:19565:2447 TAACACC -@HWI-ST575:165:C1P5BACXX:1:1101:20585:2479 CCTGACT -@HWI-ST575:165:C1P5BACXX:1:1101:20888:2259 TGCAAGC -@HWI-ST575:165:C1P5BACXX:1:1101:1310:2696 CAGCCCT -@HWI-ST575:165:C1P5BACXX:1:1101:4969:2645 CCTTAAT -@HWI-ST575:165:C1P5BACXX:1:1101:6485:2694 AAGCCGC -@HWI-ST575:165:C1P5BACXX:1:1101:6896:2699 CCAATAC -@HWI-ST575:165:C1P5BACXX:1:1101:7108:2513 CAACGAC -@HWI-ST575:165:C1P5BACXX:1:1101:7123:2615 TGTTGAC -@HWI-ST575:165:C1P5BACXX:1:1101:7199:2618 TTAATCC -@HWI-ST575:165:C1P5BACXX:1:1101:10015:2714 CGGACTC -@HWI-ST575:165:C1P5BACXX:1:1101:10105:2714 GCTGGTC -@HWI-ST575:165:C1P5BACXX:1:1101:11341:2736 TCTTAAC -@HWI-ST575:165:C1P5BACXX:1:1101:11611:2512 AGACCAC -@HWI-ST575:165:C1P5BACXX:1:1101:11611:2576 GGCTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:11522:2633 CACTATC -@HWI-ST575:165:C1P5BACXX:1:1101:12762:2599 ATCGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:13122:2666 AACCGCA -@HWI-ST575:165:C1P5BACXX:1:1101:13331:2504 CTAGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:13328:2614 TAAGCGC -@HWI-ST575:165:C1P5BACXX:1:1101:13944:2746 CCAAACC -@HWI-ST575:165:C1P5BACXX:1:1101:14544:2676 CCTCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:16211:2706 TCATTAC -@HWI-ST575:165:C1P5BACXX:1:1101:17293:2515 CTTACAT -@HWI-ST575:165:C1P5BACXX:1:1101:17341:2572 GAATGGT -@HWI-ST575:165:C1P5BACXX:1:1101:17813:2676 ACACATT -@HWI-ST575:165:C1P5BACXX:1:1101:18322:2589 CCACTCC -@HWI-ST575:165:C1P5BACXX:1:1101:18563:2613 TGCACTC -@HWI-ST575:165:C1P5BACXX:1:1101:19030:2538 ATACCCC -@HWI-ST575:165:C1P5BACXX:1:1101:19375:2502 GTAGTCC -@HWI-ST575:165:C1P5BACXX:1:1101:19630:2588 ACAACCC -@HWI-ST575:165:C1P5BACXX:1:1101:1885:2921 ACGGACC -@HWI-ST575:165:C1P5BACXX:1:1101:3712:2816 GCTTAAC -@HWI-ST575:165:C1P5BACXX:1:1101:3931:2939 TGTTCGG -@HWI-ST575:165:C1P5BACXX:1:1101:4575:2908 AAACTCC -@HWI-ST575:165:C1P5BACXX:1:1101:4670:2972 TTGTGAC -@HWI-ST575:165:C1P5BACXX:1:1101:4779:2823 ACTCGAC -@HWI-ST575:165:C1P5BACXX:1:1101:4804:2998 GTTACTG -@HWI-ST575:165:C1P5BACXX:1:1101:5202:2792 CGCACGC -@HWI-ST575:165:C1P5BACXX:1:1101:5939:2936 CTTGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:6091:2918 CGACCAC -@HWI-ST575:165:C1P5BACXX:1:1101:6392:2828 CGCGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:7180:2991 CATCGGG -@HWI-ST575:165:C1P5BACXX:1:1101:7419:2920 CGTACAC -@HWI-ST575:165:C1P5BACXX:1:1101:7772:2979 GCACCTC -@HWI-ST575:165:C1P5BACXX:1:1101:8507:2811 CGCCGCC -@HWI-ST575:165:C1P5BACXX:1:1101:8832:2869 GCCTCAC -@HWI-ST575:165:C1P5BACXX:1:1101:9485:2898 GCTTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:9990:2821 CATGCTC -@HWI-ST575:165:C1P5BACXX:1:1101:10328:2966 ATCGGTC -@HWI-ST575:165:C1P5BACXX:1:1101:11508:2796 AACGGTC -@HWI-ST575:165:C1P5BACXX:1:1101:11726:2972 GGAGTTC -@HWI-ST575:165:C1P5BACXX:1:1101:12044:2834 CCATGAA -@HWI-ST575:165:C1P5BACXX:1:1101:12169:2963 ACTCCGC -@HWI-ST575:165:C1P5BACXX:1:1101:13168:2760 CGACCTC -@HWI-ST575:165:C1P5BACXX:1:1101:13484:2768 TTTTCTC -@HWI-ST575:165:C1P5BACXX:1:1101:13399:2774 CTAGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:13467:2781 GCATCTC -@HWI-ST575:165:C1P5BACXX:1:1101:13716:2837 CGCTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:14400:2843 CAATTTT -@HWI-ST575:165:C1P5BACXX:1:1101:14816:2802 CACTGTT -@HWI-ST575:165:C1P5BACXX:1:1101:15055:2926 CTAGACG -@HWI-ST575:165:C1P5BACXX:1:1101:17639:2908 TACAGAT -@HWI-ST575:165:C1P5BACXX:1:1101:20319:2983 GGTATTT -@HWI-ST575:165:C1P5BACXX:1:1101:20783:2769 AAAACGC -@HWI-ST575:165:C1P5BACXX:1:1101:1673:3017 AATATCC -@HWI-ST575:165:C1P5BACXX:1:1101:2153:3048 ATTCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:4715:3184 TCCGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:4796:3234 CAACCAC -@HWI-ST575:165:C1P5BACXX:1:1101:5104:3008 ACGGCTC -@HWI-ST575:165:C1P5BACXX:1:1101:5272:3008 GAAGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:5594:3119 ACCCCCC -@HWI-ST575:165:C1P5BACXX:1:1101:6927:3052 CACAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:7098:3130 AGCCTCC -@HWI-ST575:165:C1P5BACXX:1:1101:7842:3018 AACCACT -@HWI-ST575:165:C1P5BACXX:1:1101:8316:3056 CTCTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:8582:3236 CCAAAGC -@HWI-ST575:165:C1P5BACXX:1:1101:10343:3076 TTCATAC -@HWI-ST575:165:C1P5BACXX:1:1101:10433:3184 CCCTTTT -@HWI-ST575:165:C1P5BACXX:1:1101:10787:3078 AGCCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:10951:3116 GGTCCAC -@HWI-ST575:165:C1P5BACXX:1:1101:11196:3016 TGCTAAC -@HWI-ST575:165:C1P5BACXX:1:1101:11257:3175 TTTTGGT -@HWI-ST575:165:C1P5BACXX:1:1101:12061:3148 CCAAGTC -@HWI-ST575:165:C1P5BACXX:1:1101:12768:3208 ATTCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:14168:3032 TCCCCTC -@HWI-ST575:165:C1P5BACXX:1:1101:14033:3072 GCAACGG -@HWI-ST575:165:C1P5BACXX:1:1101:14598:3012 TGTTTTC -@HWI-ST575:165:C1P5BACXX:1:1101:14516:3084 GACGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:15426:3038 ACAAGTG -@HWI-ST575:165:C1P5BACXX:1:1101:16490:3172 GCATTCG -@HWI-ST575:165:C1P5BACXX:1:1101:16810:3009 AGTTAGC -@HWI-ST575:165:C1P5BACXX:1:1101:17555:3239 GCTATAC -@HWI-ST575:165:C1P5BACXX:1:1101:18319:3009 ATGCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:19255:3046 CAATAAC -@HWI-ST575:165:C1P5BACXX:1:1101:20168:3013 GACTCAC -@HWI-ST575:165:C1P5BACXX:1:1101:21025:3228 TATAATG -@HWI-ST575:165:C1P5BACXX:1:1101:3940:3277 TCACGGC -@HWI-ST575:165:C1P5BACXX:1:1101:5732:3431 CAATATT -@HWI-ST575:165:C1P5BACXX:1:1101:6498:3306 AGCTCAC -@HWI-ST575:165:C1P5BACXX:1:1101:6927:3383 ATACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:8668:3366 AACCCTC -@HWI-ST575:165:C1P5BACXX:1:1101:9555:3271 TCGCCAC -@HWI-ST575:165:C1P5BACXX:1:1101:9960:3491 ATATCGC -@HWI-ST575:165:C1P5BACXX:1:1101:11371:3324 TTCGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:11999:3288 CCATATC -@HWI-ST575:165:C1P5BACXX:1:1101:12147:3289 TCCAACG -@HWI-ST575:165:C1P5BACXX:1:1101:12666:3312 TTACATG -@HWI-ST575:165:C1P5BACXX:1:1101:12701:3456 CCAATCC -@HWI-ST575:165:C1P5BACXX:1:1101:13062:3317 TTTCTCC -@HWI-ST575:165:C1P5BACXX:1:1101:13545:3337 CAACTAC -@HWI-ST575:165:C1P5BACXX:1:1101:14345:3429 CCAATAT -@HWI-ST575:165:C1P5BACXX:1:1101:14585:3422 AGATTAC -@HWI-ST575:165:C1P5BACXX:1:1101:16628:3381 TTCCCTC -@HWI-ST575:165:C1P5BACXX:1:1101:1080:3578 TACGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:1525:3749 TGCGGCC -@HWI-ST575:165:C1P5BACXX:1:1101:2431:3630 AACCCCC -@HWI-ST575:165:C1P5BACXX:1:1101:2802:3618 TGACTTT -@HWI-ST575:165:C1P5BACXX:1:1101:3064:3520 CCTGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:3610:3614 GCGCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:4225:3617 TCGAGCT -@HWI-ST575:165:C1P5BACXX:1:1101:4165:3628 ACCATAC -@HWI-ST575:165:C1P5BACXX:1:1101:4951:3655 CGACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:4878:3725 TACATAT -@HWI-ST575:165:C1P5BACXX:1:1101:5530:3596 CTCGTTT -@HWI-ST575:165:C1P5BACXX:1:1101:6120:3600 ACAACAC -@HWI-ST575:165:C1P5BACXX:1:1101:6900:3547 CAAGCAT -@HWI-ST575:165:C1P5BACXX:1:1101:7633:3566 CAACACT -@HWI-ST575:165:C1P5BACXX:1:1101:9213:3506 TCCCCCT -@HWI-ST575:165:C1P5BACXX:1:1101:10414:3701 GCAATTC -@HWI-ST575:165:C1P5BACXX:1:1101:10901:3561 GCTACCT -@HWI-ST575:165:C1P5BACXX:1:1101:10915:3580 CTGTACA -@HWI-ST575:165:C1P5BACXX:1:1101:11184:3661 CCTATTC -@HWI-ST575:165:C1P5BACXX:1:1101:11415:3524 CTAGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:12086:3581 TCCATCC -@HWI-ST575:165:C1P5BACXX:1:1101:12420:3632 ACCTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:13315:3747 TCAGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:14374:3673 CATGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:14502:3661 ACACAGC -@HWI-ST575:165:C1P5BACXX:1:1101:14952:3691 CATCATT -@HWI-ST575:165:C1P5BACXX:1:1101:15058:3596 AGGCTGA -@HWI-ST575:165:C1P5BACXX:1:1101:17467:3686 ACCAAAT -@HWI-ST575:165:C1P5BACXX:1:1101:17669:3607 AGCCCGG -@HWI-ST575:165:C1P5BACXX:1:1101:18356:3623 CCAGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:18913:3633 TCTGAAT -@HWI-ST575:165:C1P5BACXX:1:1101:20863:3620 CCTGTAA -@HWI-ST575:165:C1P5BACXX:1:1101:1773:3939 GTGACAC -@HWI-ST575:165:C1P5BACXX:1:1101:2081:3878 TGTGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:4430:3860 GAGCCTT -@HWI-ST575:165:C1P5BACXX:1:1101:6400:3801 AGTCCCC -@HWI-ST575:165:C1P5BACXX:1:1101:6719:3898 TGACCAC -@HWI-ST575:165:C1P5BACXX:1:1101:8167:3757 AGAGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:9714:3894 CCATACC -@HWI-ST575:165:C1P5BACXX:1:1101:11290:3835 CTGCGGT -@HWI-ST575:165:C1P5BACXX:1:1101:12038:3902 GTAGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:12700:3779 CTTACTC -@HWI-ST575:165:C1P5BACXX:1:1101:12790:3907 GCTTCTT -@HWI-ST575:165:C1P5BACXX:1:1101:13831:3779 TACATAC -@HWI-ST575:165:C1P5BACXX:1:1101:14731:3871 ACGCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:14772:3935 AGCACAC -@HWI-ST575:165:C1P5BACXX:1:1101:15067:3763 GCCGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:15156:3801 CTCCGTC -@HWI-ST575:165:C1P5BACXX:1:1101:16559:3892 GCCGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:17255:3956 GCTCATT -@HWI-ST575:165:C1P5BACXX:1:1101:17500:3790 GCGACGG -@HWI-ST575:165:C1P5BACXX:1:1101:18440:3926 GAGGTTT -@HWI-ST575:165:C1P5BACXX:1:1101:19111:3987 TACCACC -@HWI-ST575:165:C1P5BACXX:1:1101:19557:3888 CACGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:19920:3801 GGGCCAC -@HWI-ST575:165:C1P5BACXX:1:1101:1580:4241 TTCAGAC -@HWI-ST575:165:C1P5BACXX:1:1101:2986:4163 TCGAGAC -@HWI-ST575:165:C1P5BACXX:1:1101:4437:4204 AACCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:5319:4088 GGTTCAC -@HWI-ST575:165:C1P5BACXX:1:1101:5702:4094 CTATATC -@HWI-ST575:165:C1P5BACXX:1:1101:5824:4111 CATCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:6984:4013 ACCGACT -@HWI-ST575:165:C1P5BACXX:1:1101:7890:4076 CCCACAC -@HWI-ST575:165:C1P5BACXX:1:1101:8052:4159 TTTTCGG -@HWI-ST575:165:C1P5BACXX:1:1101:8080:4229 TCAATCT -@HWI-ST575:165:C1P5BACXX:1:1101:8526:4228 CATTAAC -@HWI-ST575:165:C1P5BACXX:1:1101:8765:4049 TTCCCCT -@HWI-ST575:165:C1P5BACXX:1:1101:9822:4042 TCTTCAT -@HWI-ST575:165:C1P5BACXX:1:1101:10184:4066 TAAAGCG -@HWI-ST575:165:C1P5BACXX:1:1101:11794:4113 CTTTATC -@HWI-ST575:165:C1P5BACXX:1:1101:12356:4148 CGTTCTC -@HWI-ST575:165:C1P5BACXX:1:1101:14554:4015 ACGTATC -@HWI-ST575:165:C1P5BACXX:1:1101:14736:4035 ATAGCAG -@HWI-ST575:165:C1P5BACXX:1:1101:15267:4200 GATACTC -@HWI-ST575:165:C1P5BACXX:1:1101:17040:4246 TGATTAC -@HWI-ST575:165:C1P5BACXX:1:1101:17659:4106 CTGGTTC -@HWI-ST575:165:C1P5BACXX:1:1101:17880:4233 TTCAATC -@HWI-ST575:165:C1P5BACXX:1:1101:19348:4134 GATCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:1929:4262 GGCCCGC -@HWI-ST575:165:C1P5BACXX:1:1101:3262:4389 GCTTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:3996:4500 GCCAGAC -@HWI-ST575:165:C1P5BACXX:1:1101:4255:4286 TTCTCCT -@HWI-ST575:165:C1P5BACXX:1:1101:4583:4479 CACCATC -@HWI-ST575:165:C1P5BACXX:1:1101:5886:4450 AACATCT -@HWI-ST575:165:C1P5BACXX:1:1101:6699:4365 CACGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:7054:4268 CCATTAC -@HWI-ST575:165:C1P5BACXX:1:1101:7021:4304 GAAGCTG -@HWI-ST575:165:C1P5BACXX:1:1101:7330:4272 GGCCGAC -@HWI-ST575:165:C1P5BACXX:1:1101:7985:4412 AACTCCC -@HWI-ST575:165:C1P5BACXX:1:1101:9154:4399 CGTACAC -@HWI-ST575:165:C1P5BACXX:1:1101:9397:4390 TTAGCTC -@HWI-ST575:165:C1P5BACXX:1:1101:9626:4444 CATCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:9920:4375 ATGTCAC -@HWI-ST575:165:C1P5BACXX:1:1101:9928:4484 ACACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:10347:4290 TCCCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:10607:4474 ACCAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:11109:4422 ATTTTTT -@HWI-ST575:165:C1P5BACXX:1:1101:11719:4366 CTTGGAC -@HWI-ST575:165:C1P5BACXX:1:1101:11803:4357 CAGTAGC -@HWI-ST575:165:C1P5BACXX:1:1101:12622:4263 ACGTCTC -@HWI-ST575:165:C1P5BACXX:1:1101:13888:4376 TGAGCCC -@HWI-ST575:165:C1P5BACXX:1:1101:14985:4327 TAAGCCC -@HWI-ST575:165:C1P5BACXX:1:1101:15294:4416 CACGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:16020:4286 CCTCTCG -@HWI-ST575:165:C1P5BACXX:1:1101:16371:4398 GGTCGTG -@HWI-ST575:165:C1P5BACXX:1:1101:17919:4354 ACCAGTG -@HWI-ST575:165:C1P5BACXX:1:1101:19347:4445 TATGACT -@HWI-ST575:165:C1P5BACXX:1:1101:19620:4333 CTACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:20453:4255 TCACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:20959:4324 ATTCATT -@HWI-ST575:165:C1P5BACXX:1:1101:20762:4415 CTTCGCT -@HWI-ST575:165:C1P5BACXX:1:1101:21156:4355 TCGACCC -@HWI-ST575:165:C1P5BACXX:1:1101:21005:4492 AGACTAC -@HWI-ST575:165:C1P5BACXX:1:1101:2208:4721 ACCACAC -@HWI-ST575:165:C1P5BACXX:1:1101:3594:4541 CCACTGC -@HWI-ST575:165:C1P5BACXX:1:1101:4453:4680 CTAACGG -@HWI-ST575:165:C1P5BACXX:1:1101:4574:4514 TACGGCC -@HWI-ST575:165:C1P5BACXX:1:1101:4512:4554 ATCCCAC -@HWI-ST575:165:C1P5BACXX:1:1101:6091:4740 CCTGTTC -@HWI-ST575:165:C1P5BACXX:1:1101:7755:4724 TCATGAC -@HWI-ST575:165:C1P5BACXX:1:1101:10497:4685 CGACCGC -@HWI-ST575:165:C1P5BACXX:1:1101:13319:4694 CATGTCC -@HWI-ST575:165:C1P5BACXX:1:1101:14064:4581 CTGACAC -@HWI-ST575:165:C1P5BACXX:1:1101:14735:4724 GGGAACC -@HWI-ST575:165:C1P5BACXX:1:1101:15633:4606 ATGAGTC -@HWI-ST575:165:C1P5BACXX:1:1101:16660:4702 ATTAGTC -@HWI-ST575:165:C1P5BACXX:1:1101:18593:4710 TTCCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:19241:4545 AGACGAC -@HWI-ST575:165:C1P5BACXX:1:1101:19601:4684 GCAATCT -@HWI-ST575:165:C1P5BACXX:1:1101:20709:4735 TCACTTC -@HWI-ST575:165:C1P5BACXX:1:1101:21224:4560 ACTTGAA -@HWI-ST575:165:C1P5BACXX:1:1101:2354:4948 TTACGAC -@HWI-ST575:165:C1P5BACXX:1:1101:2921:4941 CGCGCAT -@HWI-ST575:165:C1P5BACXX:1:1101:4132:4919 ACGACCC -@HWI-ST575:165:C1P5BACXX:1:1101:5510:4838 CGTATAC -@HWI-ST575:165:C1P5BACXX:1:1101:6248:4986 ACAAAGC -@HWI-ST575:165:C1P5BACXX:1:1101:6936:4836 CCCTGTC -@HWI-ST575:165:C1P5BACXX:1:1101:7709:4867 ACGTGAC -@HWI-ST575:165:C1P5BACXX:1:1101:9439:4978 ACGTCCC -@HWI-ST575:165:C1P5BACXX:1:1101:9572:4907 TCAACAC -@HWI-ST575:165:C1P5BACXX:1:1101:9900:4892 CGTTAAC -@HWI-ST575:165:C1P5BACXX:1:1101:9950:4989 CAGTAAC -@HWI-ST575:165:C1P5BACXX:1:1101:10290:4901 TATGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:10794:4755 ATAGCCT -@HWI-ST575:165:C1P5BACXX:1:1101:11203:4970 ATCTAAC -@HWI-ST575:165:C1P5BACXX:1:1101:11597:4816 CTAATAC -@HWI-ST575:165:C1P5BACXX:1:1101:11814:4971 GTTGGCT -@HWI-ST575:165:C1P5BACXX:1:1101:12815:4804 GTCAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:15064:4795 TTACCGA -@HWI-ST575:165:C1P5BACXX:1:1101:15338:4874 TCCCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:15739:4984 CCCGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:16043:4995 CATAACG -@HWI-ST575:165:C1P5BACXX:1:1101:17608:4859 ACGCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:17879:4904 GTTAATC -@HWI-ST575:165:C1P5BACXX:1:1101:18072:4949 CTTAACT -@HWI-ST575:165:C1P5BACXX:1:1101:18453:4916 GGGACAC -@HWI-ST575:165:C1P5BACXX:1:1101:19254:4780 GCCGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:19861:4798 ATTGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:19870:4937 CAACTCC -@HWI-ST575:165:C1P5BACXX:1:1101:19796:4989 ACCCGAC -@HWI-ST575:165:C1P5BACXX:1:1101:20053:4972 TATGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:20782:4908 CGACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:1189:5021 ACCGGAT -@HWI-ST575:165:C1P5BACXX:1:1101:1686:5115 TCATAGC -@HWI-ST575:165:C1P5BACXX:1:1101:2938:5127 GCCTCAC -@HWI-ST575:165:C1P5BACXX:1:1101:3405:5036 ACTGATC -@HWI-ST575:165:C1P5BACXX:1:1101:7045:5134 ATGGCCT -@HWI-ST575:165:C1P5BACXX:1:1101:7123:5216 CGCCCCT -@HWI-ST575:165:C1P5BACXX:1:1101:8358:5145 CTGCGGC -@HWI-ST575:165:C1P5BACXX:1:1101:8840:5173 TACGCCG -@HWI-ST575:165:C1P5BACXX:1:1101:9731:5160 AAGGCTC -@HWI-ST575:165:C1P5BACXX:1:1101:10830:5146 GAGCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:11032:5068 GCGCAGG -@HWI-ST575:165:C1P5BACXX:1:1101:11565:5085 GCACCTC -@HWI-ST575:165:C1P5BACXX:1:1101:12209:5017 ACACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:12070:5238 GGAGATC -@HWI-ST575:165:C1P5BACXX:1:1101:12614:5234 TTACCAC -@HWI-ST575:165:C1P5BACXX:1:1101:12934:5066 TGTATAC -@HWI-ST575:165:C1P5BACXX:1:1101:12889:5153 GGCAGCT -@HWI-ST575:165:C1P5BACXX:1:1101:13006:5008 CCCCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:13701:5054 AATCAGC -@HWI-ST575:165:C1P5BACXX:1:1101:13818:5190 CCCATCG -@HWI-ST575:165:C1P5BACXX:1:1101:14721:5211 GCCGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:15180:5074 TCTATTG -@HWI-ST575:165:C1P5BACXX:1:1101:15733:5028 TGGGTAT -@HWI-ST575:165:C1P5BACXX:1:1101:16783:5148 TACACAC -@HWI-ST575:165:C1P5BACXX:1:1101:17093:5065 CGGTCGT -@HWI-ST575:165:C1P5BACXX:1:1101:18177:5052 TCATTAC -@HWI-ST575:165:C1P5BACXX:1:1101:18454:5064 CATATGC -@HWI-ST575:165:C1P5BACXX:1:1101:19514:5155 TTCTTAT -@HWI-ST575:165:C1P5BACXX:1:1101:21070:5172 GCATATC -@HWI-ST575:165:C1P5BACXX:1:1101:1152:5446 CATACAC -@HWI-ST575:165:C1P5BACXX:1:1101:2332:5476 CACCGTA -@HWI-ST575:165:C1P5BACXX:1:1101:3549:5422 TTTCCGC -@HWI-ST575:165:C1P5BACXX:1:1101:3934:5255 TACATGG -@HWI-ST575:165:C1P5BACXX:1:1101:3779:5257 CGCGGTC -@HWI-ST575:165:C1P5BACXX:1:1101:3981:5396 TCCAGTC -@HWI-ST575:165:C1P5BACXX:1:1101:4352:5435 CTTACAC -@HWI-ST575:165:C1P5BACXX:1:1101:5757:5381 GTACATT -@HWI-ST575:165:C1P5BACXX:1:1101:7084:5290 GCATCCC -@HWI-ST575:165:C1P5BACXX:1:1101:7550:5435 CGCCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:8518:5454 GACGGAC -@HWI-ST575:165:C1P5BACXX:1:1101:8740:5499 CTTAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:8810:5337 TGCACGC -@HWI-ST575:165:C1P5BACXX:1:1101:10317:5279 TGACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:11346:5362 ACAATTC -@HWI-ST575:165:C1P5BACXX:1:1101:12293:5318 AGCGACG -@HWI-ST575:165:C1P5BACXX:1:1101:12385:5426 CAAGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:12559:5359 GCGGGAC -@HWI-ST575:165:C1P5BACXX:1:1101:13619:5383 AGCGGTC -@HWI-ST575:165:C1P5BACXX:1:1101:14813:5367 TGCCCAT -@HWI-ST575:165:C1P5BACXX:1:1101:16958:5440 TCCAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:17064:5269 CCTACAT -@HWI-ST575:165:C1P5BACXX:1:1101:18711:5374 TTGTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:19795:5388 GACTGAC -@HWI-ST575:165:C1P5BACXX:1:1101:1536:5742 ACCCCCC -@HWI-ST575:165:C1P5BACXX:1:1101:3186:5524 CTTCTCC -@HWI-ST575:165:C1P5BACXX:1:1101:3099:5544 CCAATCC -@HWI-ST575:165:C1P5BACXX:1:1101:4347:5721 ATCGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:5268:5661 TAACCAC -@HWI-ST575:165:C1P5BACXX:1:1101:6568:5658 TGCAATC -@HWI-ST575:165:C1P5BACXX:1:1101:7344:5519 CACTAGC -@HWI-ST575:165:C1P5BACXX:1:1101:8216:5553 GGTACCC -@HWI-ST575:165:C1P5BACXX:1:1101:8183:5554 CTTAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:8832:5696 AACGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:9211:5626 TACTATC -@HWI-ST575:165:C1P5BACXX:1:1101:9028:5675 TATTACT -@HWI-ST575:165:C1P5BACXX:1:1101:9789:5670 GCGTGAC -@HWI-ST575:165:C1P5BACXX:1:1101:10507:5654 TCAAACC -@HWI-ST575:165:C1P5BACXX:1:1101:12148:5538 GACCCAC -@HWI-ST575:165:C1P5BACXX:1:1101:12149:5686 ATCCAGC -@HWI-ST575:165:C1P5BACXX:1:1101:12546:5747 CACTTGG -@HWI-ST575:165:C1P5BACXX:1:1101:13615:5616 GCTATTC -@HWI-ST575:165:C1P5BACXX:1:1101:13537:5735 GCTTGAC -@HWI-ST575:165:C1P5BACXX:1:1101:14584:5542 GCCGAAG -@HWI-ST575:165:C1P5BACXX:1:1101:15724:5746 TGTGGAT -@HWI-ST575:165:C1P5BACXX:1:1101:17227:5625 ACCAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:17881:5507 TAACCCT -@HWI-ST575:165:C1P5BACXX:1:1101:20112:5554 CTCTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:20322:5548 ACCGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:21233:5679 CCCCCGC -@HWI-ST575:165:C1P5BACXX:1:1101:1959:5920 GCAGTGT -@HWI-ST575:165:C1P5BACXX:1:1101:1924:5978 GCCTCTC -@HWI-ST575:165:C1P5BACXX:1:1101:4153:5778 CGTGAGC -@HWI-ST575:165:C1P5BACXX:1:1101:4024:5788 CGTTGAC -@HWI-ST575:165:C1P5BACXX:1:1101:4463:5945 CTGCGAC -@HWI-ST575:165:C1P5BACXX:1:1101:6188:5778 GCGGTCG -@HWI-ST575:165:C1P5BACXX:1:1101:6867:5946 CCACAAT -@HWI-ST575:165:C1P5BACXX:1:1101:7535:5814 TGCAACG -@HWI-ST575:165:C1P5BACXX:1:1101:7794:5903 ACAATGC -@HWI-ST575:165:C1P5BACXX:1:1101:9106:5832 CTCGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:9392:5986 TTGACCC -@HWI-ST575:165:C1P5BACXX:1:1101:9549:5793 CTTATAT -@HWI-ST575:165:C1P5BACXX:1:1101:10541:5867 TCGCCCT -@HWI-ST575:165:C1P5BACXX:1:1101:11397:5818 AATCTAT -@HWI-ST575:165:C1P5BACXX:1:1101:13482:5805 TAAGGCT -@HWI-ST575:165:C1P5BACXX:1:1101:13731:5797 CACTAAG -@HWI-ST575:165:C1P5BACXX:1:1101:13983:5888 TGGCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:13915:5939 CGTGGTC -@HWI-ST575:165:C1P5BACXX:1:1101:14076:5802 CTCCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:14165:5883 ATGCTTC -@HWI-ST575:165:C1P5BACXX:1:1101:14716:5991 CGCCCAC -@HWI-ST575:165:C1P5BACXX:1:1101:15153:5829 CCCACCC -@HWI-ST575:165:C1P5BACXX:1:1101:15618:5813 GGGAGAC -@HWI-ST575:165:C1P5BACXX:1:1101:16278:5962 GCCGACC -@HWI-ST575:165:C1P5BACXX:1:1101:17404:5768 AGTCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:18508:5770 ATACGGT -@HWI-ST575:165:C1P5BACXX:1:1101:18654:5893 GTTGTTC -@HWI-ST575:165:C1P5BACXX:1:1101:20005:5823 TCTATAC -@HWI-ST575:165:C1P5BACXX:1:1101:20461:5768 TCTTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:1693:6048 AACCCTT -@HWI-ST575:165:C1P5BACXX:1:1101:3291:6004 CCCACCC -@HWI-ST575:165:C1P5BACXX:1:1101:3937:6198 CCTTCTC -@HWI-ST575:165:C1P5BACXX:1:1101:4273:6038 CACGTTC -@HWI-ST575:165:C1P5BACXX:1:1101:4445:6115 CTGCAGC -@HWI-ST575:165:C1P5BACXX:1:1101:4344:6153 AGTGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:5212:6077 GCTCGTC -@HWI-ST575:165:C1P5BACXX:1:1101:5854:6193 TTGACGA -@HWI-ST575:165:C1P5BACXX:1:1101:6093:6246 AAGGGCT -@HWI-ST575:165:C1P5BACXX:1:1101:8105:6158 GTCTAGC -@HWI-ST575:165:C1P5BACXX:1:1101:8253:6223 GATAGCC -@HWI-ST575:165:C1P5BACXX:1:1101:8552:6031 GCTAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:8575:6101 ACTACTA -@HWI-ST575:165:C1P5BACXX:1:1101:9569:6015 CAACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:10905:6024 GCAGCCT -@HWI-ST575:165:C1P5BACXX:1:1101:11477:6010 CCGTTGT -@HWI-ST575:165:C1P5BACXX:1:1101:12389:6154 CCACCTC -@HWI-ST575:165:C1P5BACXX:1:1101:12820:6019 ACACCCC -@HWI-ST575:165:C1P5BACXX:1:1101:13503:6047 CCGCTCA -@HWI-ST575:165:C1P5BACXX:1:1101:14071:6041 TTATGAC -@HWI-ST575:165:C1P5BACXX:1:1101:14685:6102 ACTTACC -@HWI-ST575:165:C1P5BACXX:1:1101:14930:6229 GCCCATC -@HWI-ST575:165:C1P5BACXX:1:1101:15280:6053 CCCGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:15344:6191 CAAATCC -@HWI-ST575:165:C1P5BACXX:1:1101:16410:6086 CGCTGGT -@HWI-ST575:165:C1P5BACXX:1:1101:16374:6145 CCTGTTC -@HWI-ST575:165:C1P5BACXX:1:1101:16749:6158 CGGCTAA -@HWI-ST575:165:C1P5BACXX:1:1101:17553:6192 CACCTAC -@HWI-ST575:165:C1P5BACXX:1:1101:17938:6081 TAAGTGC -@HWI-ST575:165:C1P5BACXX:1:1101:19315:6150 TTACCTC -@HWI-ST575:165:C1P5BACXX:1:1101:19532:6018 CACCCTC -@HWI-ST575:165:C1P5BACXX:1:1101:21261:6248 ATACATC -@HWI-ST575:165:C1P5BACXX:1:1101:1089:6262 AACATGC -@HWI-ST575:165:C1P5BACXX:1:1101:1439:6319 GCGGTTC -@HWI-ST575:165:C1P5BACXX:1:1101:1829:6451 CGGATAT -@HWI-ST575:165:C1P5BACXX:1:1101:2484:6444 GAATGAC -@HWI-ST575:165:C1P5BACXX:1:1101:3350:6364 CATTGCG -@HWI-ST575:165:C1P5BACXX:1:1101:3699:6336 CAGGCTT -@HWI-ST575:165:C1P5BACXX:1:1101:3874:6280 AACCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:4404:6415 CCAAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:5416:6451 CAATGTT -@HWI-ST575:165:C1P5BACXX:1:1101:7066:6455 CCTAGAC -@HWI-ST575:165:C1P5BACXX:1:1101:7488:6251 GCGAGCC -@HWI-ST575:165:C1P5BACXX:1:1101:7486:6329 TAACCAT -@HWI-ST575:165:C1P5BACXX:1:1101:7643:6450 AAAGCCT -@HWI-ST575:165:C1P5BACXX:1:1101:8454:6487 CACAGTT -@HWI-ST575:165:C1P5BACXX:1:1101:9137:6329 TGACCAC -@HWI-ST575:165:C1P5BACXX:1:1101:9587:6277 TATTACC -@HWI-ST575:165:C1P5BACXX:1:1101:9536:6348 CAATTCC -@HWI-ST575:165:C1P5BACXX:1:1101:10054:6289 ACCTGAC -@HWI-ST575:165:C1P5BACXX:1:1101:10173:6294 CAAAGAT -@HWI-ST575:165:C1P5BACXX:1:1101:10764:6333 ATTCGCC -@HWI-ST575:165:C1P5BACXX:1:1101:11047:6477 TATACGT -@HWI-ST575:165:C1P5BACXX:1:1101:11411:6372 AATCCGC -@HWI-ST575:165:C1P5BACXX:1:1101:11665:6448 CCCTGAC -@HWI-ST575:165:C1P5BACXX:1:1101:12774:6457 TATATAC -@HWI-ST575:165:C1P5BACXX:1:1101:13106:6369 CAGGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:16704:6321 CCCTAAC -@HWI-ST575:165:C1P5BACXX:1:1101:16660:6380 CACTCTT -@HWI-ST575:165:C1P5BACXX:1:1101:17964:6393 TTCGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:18173:6282 CTTTAAC -@HWI-ST575:165:C1P5BACXX:1:1101:18209:6492 TACACAC -@HWI-ST575:165:C1P5BACXX:1:1101:18546:6430 TACTTCT -@HWI-ST575:165:C1P5BACXX:1:1101:19023:6315 CGATTCC -@HWI-ST575:165:C1P5BACXX:1:1101:19100:6466 GACTCCT -@HWI-ST575:165:C1P5BACXX:1:1101:20025:6400 CTCATAT -@HWI-ST575:165:C1P5BACXX:1:1101:20043:6415 TCACCAC -@HWI-ST575:165:C1P5BACXX:1:1101:1982:6500 TCGACAC -@HWI-ST575:165:C1P5BACXX:1:1101:1934:6651 TGACACG -@HWI-ST575:165:C1P5BACXX:1:1101:3031:6587 TCCGATC -@HWI-ST575:165:C1P5BACXX:1:1101:3659:6632 TAGGCTC -@HWI-ST575:165:C1P5BACXX:1:1101:4271:6639 GCGTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:5521:6516 ACAGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:6888:6616 GCACACT -@HWI-ST575:165:C1P5BACXX:1:1101:6970:6733 CCTAGCT -@HWI-ST575:165:C1P5BACXX:1:1101:9786:6627 ATCACAC -@HWI-ST575:165:C1P5BACXX:1:1101:10466:6616 CGCCGAT -@HWI-ST575:165:C1P5BACXX:1:1101:12094:6626 GAGGACG -@HWI-ST575:165:C1P5BACXX:1:1101:13236:6588 GCTCGGG -@HWI-ST575:165:C1P5BACXX:1:1101:13123:6721 GGTAATC -@HWI-ST575:165:C1P5BACXX:1:1101:13878:6710 CGACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:14181:6588 GGATCAG -@HWI-ST575:165:C1P5BACXX:1:1101:14627:6506 TACGACC -@HWI-ST575:165:C1P5BACXX:1:1101:15075:6709 TTTCTTC -@HWI-ST575:165:C1P5BACXX:1:1101:16741:6696 GTTACAC -@HWI-ST575:165:C1P5BACXX:1:1101:16765:6674 TCATAAC -@HWI-ST575:165:C1P5BACXX:1:1101:19358:6651 GTTGAAC -@HWI-ST575:165:C1P5BACXX:1:1101:19561:6627 TTACAAC -@HWI-ST575:165:C1P5BACXX:1:1101:20819:6735 TTTGCAT -@HWI-ST575:165:C1P5BACXX:1:1101:1564:6811 TACGCAC -@HWI-ST575:165:C1P5BACXX:1:1101:1927:6913 GCTAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:2572:6857 CACCGTC -@HWI-ST575:165:C1P5BACXX:1:1101:3215:6869 TGCACAC -@HWI-ST575:165:C1P5BACXX:1:1101:3121:6916 GACATAT -@HWI-ST575:165:C1P5BACXX:1:1101:5462:6854 CACAAAC -@HWI-ST575:165:C1P5BACXX:1:1101:6911:6752 AACAACG -@HWI-ST575:165:C1P5BACXX:1:1101:8402:6992 TCCCCAC -@HWI-ST575:165:C1P5BACXX:1:1101:10199:6779 TTTGAGC -@HWI-ST575:165:C1P5BACXX:1:1101:11749:6891 GATTTAC -@HWI-ST575:165:C1P5BACXX:1:1101:12769:6834 GCTGAAT -@HWI-ST575:165:C1P5BACXX:1:1101:13711:6990 GCGCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:14111:6893 GTTATGC -@HWI-ST575:165:C1P5BACXX:1:1101:14440:6994 CTGAGAC -@HWI-ST575:165:C1P5BACXX:1:1101:15694:6869 CCGCATC -@HWI-ST575:165:C1P5BACXX:1:1101:16093:6801 ACCAGCC -@HWI-ST575:165:C1P5BACXX:1:1101:16736:6834 GACGTAC -@HWI-ST575:165:C1P5BACXX:1:1101:16688:6982 CGAAGAC -@HWI-ST575:165:C1P5BACXX:1:1101:17036:6768 ACCGAGC -@HWI-ST575:165:C1P5BACXX:1:1101:17542:6830 TTCCCAC -@HWI-ST575:165:C1P5BACXX:1:1101:18093:6840 TCTATAC -@HWI-ST575:165:C1P5BACXX:1:1101:18333:6967 AACCAAC -@HWI-ST575:165:C1P5BACXX:1:1101:19230:6988 GCGCCAC -@HWI-ST575:165:C1P5BACXX:1:1101:20486:6800 CAGTCAC -@HWI-ST575:165:C1P5BACXX:1:1101:20297:6938 AAATCCC -@HWI-ST575:165:C1P5BACXX:1:1101:20449:6990 ACACAAT -@HWI-ST575:165:C1P5BACXX:1:1101:1103:7035 GATGTGC -@HWI-ST575:165:C1P5BACXX:1:1101:1500:7064 GAGGCTC -@HWI-ST575:165:C1P5BACXX:1:1101:1577:7179 AGATATC -@HWI-ST575:165:C1P5BACXX:1:1101:2297:7148 GTAAACG diff -r fb76303acf4f -r 431aebd93843 rna_probing/test-data/k2n.txt --- a/rna_probing/test-data/k2n.txt Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,58 +0,0 @@ -1 2 3 4 5 -6 7 8 9 10 -11 12 13 14 15 -16 17 18 19 20 -21 22 23 24 25 -26 27 28 29 30 -31 32 33 34 35 -36 37 38 39 40 -41 42 43 44 45 -46 47 48 49 50 -51 52 53 54 55 -56 57 58 59 60 -61 62 63 64 65 -66 67 68 69 70 -71 72 73 74 75 -76 77 78 79 80 -81 82 83 84 85 -86 87 88 90 91 -92 93 94 95 96 -97 98 99 100 101 -102 103 104 105 106 -107 108 109 110 111 -112 113 114 115 116 -117 118 119 120 121 -122 123 124 125 126 -127 128 129 130 131 -132 133 134 135 136 -137 138 139 140 141 -142 143 144 145 146 -147 148 149 150 151 -152 153 155 156 157 -158 159 160 161 162 -163 164 165 166 167 -168 169 170 171 172 -173 174 175 176 177 -178 179 180 181 182 -183 184 185 186 187 -188 189 190 191 192 -193 194 195 196 197 -198 200 201 202 203 -204 205 206 207 208 -209 210 211 212 213 -214 215 216 217 218 -219 220 221 222 223 -224 225 226 227 228 -229 230 231 232 233 -234 235 237 238 239 -240 241 242 243 244 -245 246 247 248 249 -250 251 252 253 254 -255 256 257 258 259 -260 261 262 263 264 -265 266 267 269 270 -271 272 273 274 275 -276 277 278 279 280 -281 282 283 284 285 -286 287 288 289 290 -291 292 293 294 diff -r fb76303acf4f -r 431aebd93843 rna_probing/test-data/read_counts.txt --- a/rna_probing/test-data/read_counts.txt Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,108 +0,0 @@ -RNase_P 110 187 2 -RNase_P 111 187 4 -RNase_P 112 187 2 -RNase_P 115 187 1 -RNase_P 117 187 5 -RNase_P 117 188 1 -RNase_P 119 186 1 -RNase_P 134 188 1 -RNase_P 137 187 1 -RNase_P 138 187 1 -RNase_P 152 187 1 -RNase_P 10 187 1 -RNase_P 11 187 1 -RNase_P 15 187 2 -RNase_P 30 187 2 -RNase_P 31 186 1 -RNase_P 31 188 1 -RNase_P 18 186 1 -RNase_P 18 187 1 -RNase_P 19 187 1 -RNase_P 32 187 1 -RNase_P 33 187 1 -RNase_P 50 187 2 -RNase_P 51 187 1 -RNase_P 38 187 1 -RNase_P 39 187 1 -RNase_P 55 187 3 -RNase_P 56 187 1 -RNase_P 2 187 2 -RNase_P 57 187 2 -RNase_P 71 187 2 -RNase_P 3 185 1 -RNase_P 3 186 9 -RNase_P 3 187 96 -RNase_P 3 188 1 -RNase_P 58 187 3 -RNase_P 59 187 1 -RNase_P 4 185 1 -RNase_P 4 186 32 -RNase_P 4 187 307 -RNase_P 73 187 1 -RNase_P 5 187 14 -RNase_P 6 186 2 -RNase_P 6 187 10 -RNase_P 7 187 4 -RNase_P 8 187 4 -RNase_P 90 187 2 -RNase_P 77 187 4 -RNase_P 9 187 3 -RNase_P 78 187 2 -RNase_P 92 187 2 -RNase_P 93 187 1 -RNase_P 94 187 2 -RNase_P 100 187 1 -RNase_P 97 186 1 -RNase_P 97 187 4 -RNase_P 98 187 1 -RNase_P 101 187 2 -RNase_P 102 187 1 -RNase_P 99 187 5 -RNase_P 103 187 1 -RNase_P 104 187 1 -RNase_P 105 187 1 -RNase_P 106 187 2 -RNase_P 107 186 1 -RNase_P 107 187 3 -RNase_P 121 187 2 -RNase_P 109 187 2 -RNase_P 125 187 1 -RNase_P 126 187 2 -RNase_P 128 187 3 -RNase_P 142 187 1 -RNase_P 129 187 1 -RNase_P 145 187 1 -RNase_P 147 187 1 -RNase_P 148 187 1 -RNase_P 163 187 1 -RNase_P 169 187 1 -RNase_P 20 187 1 -RNase_P 21 186 2 -RNase_P 21 187 7 -RNase_P 22 187 2 -RNase_P 23 185 1 -RNase_P 23 187 4 -RNase_P 24 187 1 -RNase_P 25 187 1 -RNase_P 40 186 1 -RNase_P 40 187 7 -RNase_P 27 186 1 -RNase_P 27 187 1 -RNase_P 41 187 1 -RNase_P 42 187 1 -RNase_P 43 187 1 -RNase_P 44 186 1 -RNase_P 44 187 1 -RNase_P 45 187 2 -RNase_P 48 186 1 -RNase_P 63 187 1 -RNase_P 64 187 1 -RNase_P 65 187 1 -RNase_P 68 187 1 -RNase_P 81 187 2 -RNase_P 82 187 2 -RNase_P 83 187 1 -RNase_P 85 187 2 -RNase_P 86 187 1 -RNase_P 89 147 1 -RNase_P 89 187 6 diff -r fb76303acf4f -r 431aebd93843 rna_probing/test-data/reads1.fastq --- a/rna_probing/test-data/reads1.fastq Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2684 +0,0 @@ -@HWI-ST575:165:C1P5BACXX:1:1101:1666:1132 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -DHHHFGGIJEGGGHGIIJDHDFDFHAGCB=FCGGEGHHGGIGEEBBDCBBBBCC -@HWI-ST575:165:C1P5BACXX:1:1101:3207:1238 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FADFFAEGGIEGIEBHG@GEA@BBGHEF>F8?78B@FCGGICEGECCABA?BA@=?C? -@HWI-ST575:165:C1P5BACXX:1:1101:3405:1152 -TGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCT -+ -DFDFD2@CEB:<3)?EF;C:91?DGBF??BBF<0909B?DDB26= -@HWI-ST575:165:C1P5BACXX:1:1101:3512:1179 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FGGHGHJJJJJIJJIIJIJIIHJHJJJGIIJJJJJJJJJGJHIIIIGEHGFFDDBDDDDDB?ACCDDD -@HWI-ST575:165:C1P5BACXX:1:1101:4473:1182 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FFHFHF@GGIIIDII:FFBDFHGGEHGIIBGGGGGIGICGIG;DGE>CHFFCA -@HWI-ST575:165:C1P5BACXX:1:1101:5162:1092 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHGHIIJJJJJJFHIIJHIJJIJJJJJJJJJIGHJJJIJIIIIIJHHGFDDDBBDDDDDDDDDDBB -@HWI-ST575:165:C1P5BACXX:1:1101:6810:1177 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FHHFHHJJJJJJJJJIJIIJJGIJIJJJIIJJJJJJJJJJJJIJJJJIGHGFFDD?BD -@HWI-ST575:165:C1P5BACXX:1:1101:8268:1157 -TCGGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCCTCGGCGGATCCAA -+ -FGHHHHJJJJJIJJIJIJJJIIIJIBFHIJJIIJJJJJJIGDFFEEEDDDDDDDDDDDDDDDDDDDDDDD@@>C>C -@HWI-ST575:165:C1P5BACXX:1:1101:8740:1185 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FGFHHHJJJJIJJJJJJIJIGIJIJJJJIJJGIJJJJJIIIJJJJIJHHHFDDDDDD;AB@BBDCCBD -@HWI-ST575:165:C1P5BACXX:1:1101:12414:1099 -GATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGA -+ -FFHHHHJJJJJJIJJJJJJIGIJGFGGHJJJJGEIIJJJJIIHHHIIIHHHHHHFDFFFD -@HWI-ST575:165:C1P5BACXX:1:1101:14053:1112 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHFHJJJJJJHHHIJIJJIJJJJJJJGDIIJJJJJIJJJGGJIIIJHHFFDDBBD9 -@HWI-ST575:165:C1P5BACXX:1:1101:14219:1152 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJJJIHIHGIFGIIJJJJJJIIJJJJJJIJIIJFIIJIJJHHFFDDB??C9A -@HWI-ST575:165:C1P5BACXX:1:1101:15012:1211 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FDFHHHIJJJJIJJGJJIIJJJJIJJJI@EGIJJJJJJHGJJIJHIFHHFFDDDBACA3> -@HWI-ST575:165:C1P5BACXX:1:1101:19911:1138 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHDHHDGIHIIIIIIDGGHIICGFHIIIGIIIIIIIIIIIIHHIIHHIFFFFCC?=CC; -@HWI-ST575:165:C1P5BACXX:1:1101:1311:1500 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT -+ -FHHHHHIJJJIJHHBGGHGIICHGFHIJJIHHIIIEIJHIIJIHGHFFHFFDDDBB?ABBDD@CDCBBDCDDDBB1=;;5 -@HWI-ST575:165:C1P5BACXX:1:1101:5495:1254 -ACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAA -+ -FHHHGHJJJJICFGGIJHJJJFFFHIJGHJJJGIIIIIIJIFFFDDDDDDDDDDDDDDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:5684:1314 -AGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC -+ -FHHHGFHHGHIIJIJJIIJJJJJFCHHIHJIJIJCHIJJJJJIGGIGHIJJIGIHHHHHFFFDD -@HWI-ST575:165:C1P5BACXX:1:1101:6002:1450 -GCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC -+ -FHHHHHJHIIJIJJIIIIJJIGHIIJJJJJBGHGJJFGDEGEFHGIBEHHCEFFFCADDBDD@BBBB -@HWI-ST575:165:C1P5BACXX:1:1101:6163:1455 -TTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG -+ -FHHHHGIJIJIIIIJICHCHGIHIJIEGHHIIJGHGHFHIIJJIF4ADDDBDDDDDDDDDDB@DCCCCDCDB -@HWI-ST575:165:C1P5BACXX:1:1101:6370:1342 -GAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG -+ -DBFDHFGIB??EFH?FGDHHB?D@B@DAHGIIIGGII9@7CAFFCAHADCCH?CACD -@HWI-ST575:165:C1P5BACXX:1:1101:7500:1303 -TTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGA -+ -FHHHHGJHHJJJJJJHJIJJDGIGIIJIIJJIIJJJIJHHIJIJJIJJGIIGIIHDEHFFFFDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:8186:1308 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGAAGGAAA -+ -FDACFFHIEIGIIE8CHE6BDFGEDCBBD@BCFHHHIDHIIIHBCH>=CD>9?779?DB?39< -@HWI-ST575:165:C1P5BACXX:1:1101:10737:1349 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAAGCCTACGT -+ -FHDFHHIJJJJJIIIJJGGIGIIGIJJIIJIJJJIJJJHHHFEFFFFECDDDDD95? -@HWI-ST575:165:C1P5BACXX:1:1101:11993:1308 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FHHHADGIIIGIHIJJJEGGGGIGIIIJIJJJJJGGGGIIIIIFHHDHFDADDB?ACDBD -@HWI-ST575:165:C1P5BACXX:1:1101:12260:1420 -TGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJJJJGIJJIIJJJIIIJJJIHGJJJEIHGGHJJJJIIGFFFDEED;;=9?CCCDB -@HWI-ST575:165:C1P5BACXX:1:1101:13041:1365 -GGGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FAHFFHGFGIJJJIJGHIIIGIJIIAHIICFGGJG@@DHHIEHFEHEDFCDDD@BB19>CBD -@HWI-ST575:165:C1P5BACXX:1:1101:13915:1290 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHGFHHJJJJIJIJJEHIHIJIJEIJIJJGGIIJIIIIJJJIEIIJJIIJHHFDDBBC??@BDBDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:13944:1432 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHGHJJJIFIIJIJJHJIIJIIIIIJJJJJJJIJJJJJJJGJIJHHHFFDDDBBC(9 -@HWI-ST575:165:C1P5BACXX:1:1101:14362:1371 -ACGACGCTCTTCCGATCTTCACCACAGACG -+ -DDHDHHIBE@EFHBC;??D90??DBBGHHI -@HWI-ST575:165:C1P5BACXX:1:1101:14865:1283 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTAGAGGCTGACGGCAGGAAAAA -+ -DDF=?CCGBGHHGD8E@E8C@D@F<@;? -@HWI-ST575:165:C1P5BACXX:1:1101:15341:1459 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FHHHHFGIIIJJJJIIJGGFGFHHGGJIIGIGJGIJJIJJGJGIIJHHHFFDDDBAC -@HWI-ST575:165:C1P5BACXX:1:1101:15856:1385 -TAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAA -+ -FHFGDFGIJIIIGIJJJGEIGJIHIJDIHHIJJJJJIIEHHHEEDBDFCECDEEECDC -@HWI-ST575:165:C1P5BACXX:1:1101:16168:1446 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJIIJJJJJIJIHIIIJJIIJJIHIJJJIIJJJJJGEIIIHIJHHFFDDDBDC? -@HWI-ST575:165:C1P5BACXX:1:1101:16274:1448 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHGFHGHEHIGIGII;GFHIIIGIJGHIIGIGGIJGIIJJIIIIJEHHFBBD;?C5A -@HWI-ST575:165:C1P5BACXX:1:1101:17532:1255 -TGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGA -+ -EFHB?FEGCGHIIIIIFHGIIIDEHGDG:?DGG?GHIHGG@HGIHFGCHGAF=@FE -@HWI-ST575:165:C1P5BACXX:1:1101:19852:1368 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHFFHHIGIJIJIIHJJGIGIADFGGGHIJIIJJJHIJIJIJGGIJGJJHGFDDC?CC9 -@HWI-ST575:165:C1P5BACXX:1:1101:20338:1414 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAG -+ -DDCDDCAEEEEIII1)::9D>BDBDICDDI;DDDBCDEBDCEIIIDCDDDD8? -@HWI-ST575:165:C1P5BACXX:1:1101:1782:1682 -GGTCGTGCCTAGCGAAGTCA -+ -DDD1C0C+22?A@BABDC?BB -@HWI-ST575:165:C1P5BACXX:1:1101:2796:1586 -AGGTCGTGCCTAGCAAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGA -+ -FHHHHHIJIJJJJJIJIIHIJIJJIJJJIIIJJJJIJJIJFJIGIJJGJGHJJHFFFFCDDB;?ACCBBDDDDA@< -@HWI-ST575:165:C1P5BACXX:1:1101:3075:1523 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHAHFHGGHIIJJJIJJIHIIGICHHIG@HIJJHHIJIJJIHIHIIGHEFEDDB?ACC -@HWI-ST575:165:C1P5BACXX:1:1101:4251:1515 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHFHHFIJJJJIJJJIJIHIIJJIJJJJJJJIJJGIJJJJIIGIJIIHHHFFDD=BD?B -@HWI-ST575:165:C1P5BACXX:1:1101:4481:1718 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT -+ -FHHHHHJJJJJJJJJIJHIJIJJIJJJJHJJJIIJJJJJIGIIJJJJHFFFADDDDDDDDDDDDDDB@ADDCBD82 -@HWI-ST575:165:C1P5BACXX:1:1101:4978:1615 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJJJJJJJJJIJIIIIGIJJJHIJJJJIJJHHGGHGDFFEECDDDDD?BCCBDB?BDCDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6935:1687 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FGDDHHHIJIJIJHGGGIFE5=AB -@HWI-ST575:165:C1P5BACXX:1:1101:7646:1624 -TTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACC -+ -FHHHHHJJJJJJJHHJJJJJJIJJJJJJJJJJJJJJJIJJJJJIHIIJJJIIGIJJIIJHFFFFEEDDDBD?;2 -@HWI-ST575:165:C1P5BACXX:1:1101:8080:1642 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHFJJJJJIJJJIIIJJJJJGJJJJIJJJJJJJJJIJJJIJJIJJGHFBAB@DCD -@HWI-ST575:165:C1P5BACXX:1:1101:8452:1710 -AAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FHHHHHJJJJJJJJJJJJJIJIIJIJJJJJIJJJIJJJIJJJJIJJICEHHCFEFEDDDD?BDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:8575:1513 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHDHHFHGIB>GGHGAGFEEGHC>D9?@CHIIEGGIIIGGGGHBGE>EEDFDDB??CD -@HWI-ST575:165:C1P5BACXX:1:1101:8610:1649 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT -+ -FHGHHHJJJJJJIJJJJJJGIGJHJIJJJIIJJJJJJJJJIJIJIIEEHFFDBB=BDDDDDBDDDDDDBDDCD<82 -@HWI-ST575:165:C1P5BACXX:1:1101:9515:1701 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA -+ -DHD@@DEG@FAGHII@DE1B>FBDDFE<=FF@;AC@DHG>7?7A==@>>AA>??B8< -@HWI-ST575:165:C1P5BACXX:1:1101:9782:1735 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FHGFHHJJJJIJJJJIJJAGIIHIGHGIIIJJJJJJJJGIIJJIJHHHHFFDA>@?B> -@HWI-ST575:165:C1P5BACXX:1:1101:10892:1546 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHFHHHIIIIJJGIFHIIIJIJJJGJJJJJJGJJIJJJGGIICHIJJJJJHDFFACE3?B:?BDDC?A -@HWI-ST575:165:C1P5BACXX:1:1101:10875:1631 -CGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGCAAAAAGCCTAC -+ -FFHHHHJIJIEIIJIHIIJJJJJIIIIIJIHIIJIJIIIGHIIJIJBEHFF';AE@B?ABDDD -@HWI-ST575:165:C1P5BACXX:1:1101:11536:1555 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGAGGGCAGGAAAAAAGCCTACGT -+ -FHHHGHJJJJIIJJJJJIJIIIJGIFIJJIJJJJIIJJIGIJIGHIHHHFFDDCDBCD@BB?ACACBB -@HWI-ST575:165:C1P5BACXX:1:1101:11540:1624 -GGGGCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHHHHFFFFFDDDDDDEDDDCCDDDDBDDBCDDEDDDDDDDDDDDDDDDDBBDCCB -@HWI-ST575:165:C1P5BACXX:1:1101:12292:1617 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHGHIIIJJIJJJJIIHGIGIEIJIIEGIJJJJEIJJJIJIIJIFFEFFDDDBDDDBBB98>>CBA -@HWI-ST575:165:C1P5BACXX:1:1101:13423:1569 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJJJJHIHIIIIIIIIIIJJJJJJJJJIJJJJIIJGJIIIJHHFFDC???CBDB@DDCDDB -@HWI-ST575:165:C1P5BACXX:1:1101:13576:1612 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHGHGHIJIJJJJJJJIIJIIJJGIJIJJIJJJGIJJJJIIJJIHHFEEDDDDBBBDCABB8?CC?>< -@HWI-ST575:165:C1P5BACXX:1:1101:14491:1526 -CGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHGFHHEHIIJIJJJ@GHAFIIJDHIGIIHHIGIIGGIIJJJJDIIJGHHFFDBB?A>? -@HWI-ST575:165:C1P5BACXX:1:1101:15109:1589 -CCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG -+ -FHGHDFGIHIGHIGIJGIJJGJBGGGGHHHFFDB=@DCCDDA@FEHIDHIJJFHGHGHF6?B39>=5>(5 -@HWI-ST575:165:C1P5BACXX:1:1101:17059:1656 -GGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTTAGAGTGGAACTCGGTAAACCCCTC -+ -FHHHHHJHGIJIIJIIJJJIJIJIJEIGHJJJIIJJJJJJJJEIICHEHIHIGGAHHHHAAEEDFFDCCEAC?BDD -@HWI-ST575:165:C1P5BACXX:1:1101:17823:1711 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT -+ -FHHHHFIJJJIJIIFGI=GIHJJJIGIJJJJIIGHIJJJJGJJGIJJHHHFDDDDDC59@@?BDDDDBBCBDBD@? -@HWI-ST575:165:C1P5BACXX:1:1101:18750:1719 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FHHHHDGEHIEIBGBEEDHGGGIHGGEHIGIGGGIIJIHIEGECG@EEEEF?>C(5>,58 -@HWI-ST575:165:C1P5BACXX:1:1101:19397:1535 -TGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJJIJIJBEH?FHGADDGHJIJIJJIFHIJJJIIJI?HIIJIIJFFFCAEC; -@HWI-ST575:165:C1P5BACXX:1:1101:19270:1553 -TCTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTT -+ -DHHDHHGHIIJJIEHHGIJJIGGCGGFBGHJGHIJIJGGIGHHHC>BDEED6@CACDC -@HWI-ST575:165:C1P5BACXX:1:1101:20160:1653 -GCTCTTCCGATCTGGACTCTCGG -+ -DHHHFHHIGIJJIHGGGGGIIE7 -@HWI-ST575:165:C1P5BACXX:1:1101:20348:1677 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHCCFGIIGIIJEGGHIGGIGIJJEHJIJIIGIIIGIJJJIJJIJIGGIJHFFDBC?(==@AGECF??BDFBE39BDDABABBF>FFFCFCCEEFCEEFDCCB<;?; -@HWI-ST575:165:C1P5BACXX:1:1101:2146:1765 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHFHFHIJJJJJJJIJIIIGIGIGHIJJJJJJIIJIJJIJIJJFIJIEEHFDDD@BCDDDBBDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:2817:1860 -TTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATC -+ -BFBBFDHIIB;FHFEAHIGH;FDCHH?;FFH@;=8=C@6;D?EHA3?;?B?6>C> -@HWI-ST575:165:C1P5BACXX:1:1101:4121:1770 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHFHFFIIIIGIIJIIICHHGJJHIEGIGHIJJIGIGIHIJJEEHIJIHFFFDD7?C;AB@AACCD>B -@HWI-ST575:165:C1P5BACXX:1:1101:4015:1808 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FAAFHDFHGGGGGJJJIJCDGEHAFFHHIIIEEHADHGCE@G@HHCAEBEF>>:7=AAAB6ABCCC@? -@HWI-ST575:165:C1P5BACXX:1:1101:4671:1789 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FG>CDHGHGGEGGGIGHIBFIHHGGCEBGGGIGI@D@EHCGIBEFHF@DB@B;>BA -@HWI-ST575:165:C1P5BACXX:1:1101:7320:1866 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FHGFHHIIJJJJJJJJJJGGIIJJJJIIJIJJIJIIGIJIIJFGHIFHHFFDDDDBDD -@HWI-ST575:165:C1P5BACXX:1:1101:7508:1798 -TCCGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHFHIJFJIIJJJJHGIHGGIJIDHIJIGIJJJJIJJJJFIIFIIHHHFFDCDDDDACD -@HWI-ST575:165:C1P5BACXX:1:1101:9647:1841 -ACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGA -+ -FHHHHHJIJJJJJJIJIJJJJGGHIJGIJJFGIIJJJIJJJJIJIJIHIJIIJJJJJDH -@HWI-ST575:165:C1P5BACXX:1:1101:11078:1760 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHFHHJIJIIJJJJGJJGIJIJJJIJJJIJJJJHIIJJJJJIJJHHHHFFDDDDBB9AABBA?CDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12091:1910 -TCACAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FHHHHHJIJJJJIJJJJIJJJIJJJJJJJJJIJIJJJIJJJIHHHFFFFDEEEDCDDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:13182:1885 -GGTCGTGCCTAGCGAAGTCATAAGCTAGTGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHGJJIJJJJJJJJJJIIIJIJJJJGGIIJJJJJJJJIJIJIIJJJHHFFD@ACCB -@HWI-ST575:165:C1P5BACXX:1:1101:13475:1836 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJJGIJJJJJJJJIIJGIIJJJIJJJJJJJJJJIJIIIJIHHHFDDDBDDDAB?9?CCCDB -@HWI-ST575:165:C1P5BACXX:1:1101:14275:1813 -AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHHIJJJJJJJIJJGIIIIDGIJIGHGIIJGIIIJJIIIIJJJIJIHHFDDAB;?;?BBBCDABA -@HWI-ST575:165:C1P5BACXX:1:1101:14914:1754 -TCAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC -+ -FHHHHHJJJIIJJJJJIJJJJIJIJJIHJJIJJGGIIGIIJIJJIJJIIIJHGGFFDDDDDBDD -@HWI-ST575:165:C1P5BACXX:1:1101:15826:1814 -TCTGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG -+ -FHGHDHGHHIIIIGIIIJGHGIEIHGGIF>AECBB?CDDCDD@; -@HWI-ST575:165:C1P5BACXX:1:1101:16316:1995 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -DHHFFHGFFGGHHIEGGFHGIIIIJJ>DGHGIIGIIIIGIHIGHIIIDDGHHHHHFFC?B8(BAACDDBD -@HWI-ST575:165:C1P5BACXX:1:1101:1102:2244 -ACGACGCTCTTCCGATCTTCCTGCTAGACGTGG -+ -D=DF8D:CEFF@GG6FGD>@CHGED>;AADBAB';5? -@HWI-ST575:165:C1P5BACXX:1:1101:3289:2146 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGC -+ -?FDFDHIIGHGIFGGGGGHGIEGHFHIGGGHGHGGC?FHI@G>FGEGGEAE -@HWI-ST575:165:C1P5BACXX:1:1101:4895:2080 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FGHGHHJJJIJJFIJHJJIJJIIIIJJGHIGIJJGIJJJJGJEIJJIIJHHF8A@?B>C -@HWI-ST575:165:C1P5BACXX:1:1101:4932:2108 -CGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG -+ -DDDDFFGEHGA=GHII@6@0DFCH>@AAAEB8@BB(>3:C@@- -@HWI-ST575:165:C1P5BACXX:1:1101:5806:2073 -ACGACGCTCTTCCGATCTGTACCATCAGACGG -+ -DHHGHAHBHIGIGDABACGF@DFD@;?>FHA: -@HWI-ST575:165:C1P5BACXX:1:1101:6214:2065 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHDHFDHIJJJJGIHGGIHGHJGEHIGIBGGIJDDHIIJJIJJJIIJGFEEDAB=ACD@ -@HWI-ST575:165:C1P5BACXX:1:1101:6143:2137 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGG -+ -DAF@DFCGDHIBG?DGIGDFEHEF>CDGD?CCFB;FA@F>GG9CHHC=?B> -@HWI-ST575:165:C1P5BACXX:1:1101:6241:2199 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -DHHHHHIJJJJIHIHJIGIIJJJJJIJJIJIIJGIIEIICGHCIIJJHHGDCB8=ACD -@HWI-ST575:165:C1P5BACXX:1:1101:7894:2030 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHIIJJJJJJIJJIJEGIJIJJJJIJJIJJJJJJJIIIIJIJHHHFFCDD?ACDDDBDDDDCBB -@HWI-ST575:165:C1P5BACXX:1:1101:8072:2245 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHHJJJJIJIJJJJJJEIJJJJJIHHIJJJJJJJIIJJJFIGHEEFFFDDD?AA? -@HWI-ST575:165:C1P5BACXX:1:1101:8299:2087 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT -+ -FHFHHDHJJJJJIIIIDGIGIJGIJIJJFIIJJIGGJJJJIIIHHHFFFFDDBD@DDDDDBDDDDCBBBDDCDB88 -@HWI-ST575:165:C1P5BACXX:1:1101:8471:2183 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT -+ -FHHHHHJJJJJJJJIJJIIFHGIIJJJJGIJIJJIJJIIIEIIJIJJJJJHHED;AEED==@DDDCBDBDDDD<88 -@HWI-ST575:165:C1P5BACXX:1:1101:9051:2011 -GTGCCACAGTGACGAAGTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCAGA -+ -FHGHHGJJJEHIIJJJJIJIJIJIJJJJGJGIJJIIFGIIFGGGGJJIHHHHFFFDB6?BBDBDDDDBBDDDB95< -@HWI-ST575:165:C1P5BACXX:1:1101:11298:2099 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTCTAGAGGCTGACGGCAGGAAAA -+ -FHHHHHJJJIGIJIJHIIEGEGHIGHIIBGIJGIIIIJJIJIIJHHHDDFDDDDB?BCC -@HWI-ST575:165:C1P5BACXX:1:1101:11886:2227 -GTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG -+ -FHAFHGJJIIFFHIJIJGIIGHGBGEHIHFCBBDABD>?@@87A99>CC?< -@HWI-ST575:165:C1P5BACXX:1:1101:12469:2156 -GGTCTTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAGCCTACGT -+ -FHHHHGJJJJJJIGIIJIJJIJJIJJJJJJJJJJIJJJHIIIIIJJIJIHHFF@?BCCB?=ACD<=? -@HWI-ST575:165:C1P5BACXX:1:1101:13307:2211 -TTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAG -+ -FHHHHHIJJHJJJJJJJIJJJJJJDBFHIJJJJIJJJIHGGFFFFFCBACECEDEDACACC -@HWI-ST575:165:C1P5BACXX:1:1101:13967:2112 -TGTGACGAAGTCTCACTAGAAATGGTGAGAGGGGAACGCGGTAAACCCCTC -+ -DA=DFA;<@B<:AA:A@HE;:BD -@HWI-ST575:165:C1P5BACXX:1:1101:14357:2064 -TGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAT -+ -FHHHHGIJIJJJJJJIIIIJIJJ9EFHIJGHHGGIJJJJIGIJGGGIJHGFFFD>BDDDCDDDDDDDDD@BDA@C4 -@HWI-ST575:165:C1P5BACXX:1:1101:14498:2106 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJJJJJJJJJHIIIJIIJJJJJJJJJJIJJJJJGHIJJJJIHHFFDDDBD3= -@HWI-ST575:165:C1P5BACXX:1:1101:14632:2166 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHGFHHHJJJIJJJJIJJGGJIJCHIGIGHJJJJJJJJJJJIJIIIIHHHFFDDD@B?CA -@HWI-ST575:165:C1P5BACXX:1:1101:14873:2011 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHHJJJJJIIJIIJHIJIJJJJJJJIJJJJJIIJJJJIJIJIJJJHHFFDDDBD= -@HWI-ST575:165:C1P5BACXX:1:1101:15054:2152 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHFHHDHIIEHIHIIGBBFCIJJIGJIIJGIGIIJHHHEHB>@DEEDEDDDDDDBBCCB -@HWI-ST575:165:C1P5BACXX:1:1101:15855:2031 -TGACCGATCCGCTTCGGCGGATCCAAAGCGG -+ -FHHGHHJJIJJJIJIDHGGHEGIHFGEEDB? -@HWI-ST575:165:C1P5BACXX:1:1101:15913:2171 -AAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -BF><; -@HWI-ST575:165:C1P5BACXX:1:1101:16436:2126 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGG -+ -DFA@@D@FCFAGBDDHD@8?BB?F9<94.828;@8CE@G;DEC9EHEBCDC -@HWI-ST575:165:C1P5BACXX:1:1101:16535:2143 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAG -+ -DDF:CFE+2AFGCGFG<:DBFFDFEB?5<:5<= -@HWI-ST575:165:C1P5BACXX:1:1101:17878:2158 -GGGTCGTGCCTAGCGAAGTAATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHFDDH:FHGJIIEHG:G?GHAHIGIEEIHFE=GDHHIJJIIIIEHHHGB@BB@?> -@HWI-ST575:165:C1P5BACXX:1:1101:2284:2369 -GGTCGTGCCTAGCGAAGTCATA -+ -FDCDFFGHGHEGGGEA<<;?@>>@3;;(5=B?BB>@BBB?0<5@@@= -@HWI-ST575:165:C1P5BACXX:1:1101:12227:2444 -ATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAAC -+ -FGHHHHJJJJJCHIIJJJIIIJJFHIIJIJJHIGIIHIIHHEHIIIIJHIJJJIHIEHGDDG=D@? -@HWI-ST575:165:C1P5BACXX:1:1101:12315:2285 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -DHHGHHJIIGHHFHHGIEIIFHIEEHIJHIJGIHAGGHIHHIDGIIEH:EDBCDBA> -@HWI-ST575:165:C1P5BACXX:1:1101:12492:2350 -TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FGHHHHJJIJBHHIJEFIIIHGIIHCHIJJJIFGIGEIGHGIIHIFHFHFFFFDCCDBDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:13583:2475 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAACTGACGGCAGGAAAAAAGCCTACGT -+ -FHGHHHJJJJJJIJJIJIGIJIIJIIIJIIGIIIHGHIJIGHHHHCFEFCDDDBBBCDBDBA@CCCBB -@HWI-ST575:165:C1P5BACXX:1:1101:14469:2354 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHFHFGHIIJJGJJJGBFGIGGCFGIHAEGGIJJJGHGHHIIIJEDHHEEBDDD9?= -@HWI-ST575:165:C1P5BACXX:1:1101:14989:2278 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJJJJJJGIJGHIHIJIJJJIIIJJJJJJJJJJIIJJJJJJJHFFDDDDDAB -@HWI-ST575:165:C1P5BACXX:1:1101:15188:2338 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG -+ -FHHHHHJJJJJJIIIJJHGHIJJJJJJIIIJJJJJJJJJJIJJJIJHHGFFDDDDBDCB8;?BDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:15331:2257 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHHJJJJJJJJIJIIFGCFFGHGHIIIJGEGHIHJJIGGIFEHGHJFFHFFDDE>C -@HWI-ST575:165:C1P5BACXX:1:1101:15849:2368 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHFHHHJIJJJJJJJJJJJIIJIJJJJHGGIJJJIIIJJIIIIHHHGFFFDDDD?B?C5 -@HWI-ST575:165:C1P5BACXX:1:1101:16152:2410 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA -+ -FHHHGHEGIIJIGJJBHBHIIJJJGGIGIJFGIHGEFHIGIIFGIJHHHFFC?BB? -@HWI-ST575:165:C1P5BACXX:1:1101:16935:2365 -AAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGGGC -+ -FFHHGHJJIIJJIIJJJJDEGHJIGEHGCHIGJJJJJJGHCHHIJIJFEDDDBDDDDDDDBDD@DBDDCCCCB959 -@HWI-ST575:165:C1P5BACXX:1:1101:17289:2412 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHHIJIIDIJJEHIDGEGIGGIJJJJJJJJHJIJJFIIJIIHHHHFFD>BB???99 -@HWI-ST575:165:C1P5BACXX:1:1101:18384:2478 -GCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCT -+ -FGHGHHFGHIJIJIJJJJJJIJDGIJJIJIJIJJJJJJJJIJJFHCHFDDCEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:19068:2414 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHFHHIIIBHHIII:EHFGIEIII*?FHDHHIIFAHCHIIGIIIIIGCHHFFCC15?;?@B<CD -@HWI-ST575:165:C1P5BACXX:1:1101:20888:2259 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTA -+ -FHH@FFHIIIIGIBF6?DDGI9FIFB=?C -@HWI-ST575:165:C1P5BACXX:1:1101:10015:2714 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHDHGHJIJJJJJJHIJJJIIIJIJJJIGJIJJJJJJJJJJJJJJJHHHFFACDDDD(< -@HWI-ST575:165:C1P5BACXX:1:1101:10105:2714 -GTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAG -+ -FHHGHHJJIJGIIFGIIIIIJJJHIIJJJJHGIIGH@BHIJIJHIJIEHFFECEC?8 -@HWI-ST575:165:C1P5BACXX:1:1101:11341:2736 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHGHHIJJJIJHIIIIIIIHGJHJJJJ2BFIIJIIJJJIIIIIIIDIHHFFDDBBDDBDDBACCDDD -@HWI-ST575:165:C1P5BACXX:1:1101:11611:2512 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGGGAAAAAAGCCTACGT -+ -FHHHHHEHJJJIJJJIJJIIJGIIJJJJIIIJJJGIIJJJJIJIGHGGFFFDD98AAA@7?ACDC@A -@HWI-ST575:165:C1P5BACXX:1:1101:11611:2576 -AGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCG -+ -FHHFHHJJJJGGIJJJDIGIIIIJGHIGGIJIIED?GHIDGHGIIGIJIGGHFHEEDFFD -@HWI-ST575:165:C1P5BACXX:1:1101:11522:2633 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHIIIJIJJJJJJIJGIGIHJJJJIIJJJIIIJJJJIJJIJIIHHHFFDDBDCCDD@EHIGHHEHH -@HWI-ST575:165:C1P5BACXX:1:1101:13331:2504 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHGHIJIJIIIIIJJJIIHGIHIJJJJJJIIJIGHIHIHIJIGIDHHFFFDDB@CCBD?,C -@HWI-ST575:165:C1P5BACXX:1:1101:16211:2706 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHAHHE1AEHIIIHIIHIGHIIIGIIGHGHGIGGGIIGIHGGIIIGGFHF:AD;ACCD -@HWI-ST575:165:C1P5BACXX:1:1101:17293:2515 -TGTTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHDHHHIJJIJJJJIHJJJJIIJIIIJGIIIJJJHGIJJJJIIIGIJIJIJFFBF?A6;;?@BDDDDBD -@HWI-ST575:165:C1P5BACXX:1:1101:17341:2572 -CGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG -+ -EHFHGFGBHHIEIIB>>@@B?ACCD@ -@HWI-ST575:165:C1P5BACXX:1:1101:17813:2676 -TTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTT -+ -DH?FD>4EBEEEBC/5=??@ -@HWI-ST575:165:C1P5BACXX:1:1101:18322:2589 -TCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGA -+ -FDFHBHGIJIIIIIGHGHAFHIGIJIGIGGGHIJJJIJGHIGIEIIEGHEAFHGIAE4CC@7AEEE -@HWI-ST575:165:C1P5BACXX:1:1101:18563:2613 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHHIJJJIIJJJHIJJJIGIJGIJIJJJJJJIJIJJIJIIHIIHHHHFFDDD@?39 -@HWI-ST575:165:C1P5BACXX:1:1101:19030:2538 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -DFF<<@CF?B@BD:?GFA89BBFF???BB=F@AB@D@AEFE>=AED>67399;;@8BD?FHIIIIAHAGIIEGI;DGHHHEEFFC?BB<5?A -@HWI-ST575:165:C1P5BACXX:1:1101:1885:2921 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FHGFFHGHIJJIJHIHIIFHIEGIHIIIIIIJJJHCHHGHHHHFFFFCEDDBDDD@BCDDD -@HWI-ST575:165:C1P5BACXX:1:1101:3712:2816 -AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHGHIIIJIJJJIIJJIJIJIJJIIIJIJJJJJJJIJJIIIIIJGIJJHFFDDECC -@HWI-ST575:165:C1P5BACXX:1:1101:3931:2939 -TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -DFFHHGGGEHFHIGA?FHAGCBGIHABFHEGCHFHFGGEEGIGIIGEH>DDD1??C=? -@HWI-ST575:165:C1P5BACXX:1:1101:4575:2908 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FFDFFHEGIGIIIIIIHDGHIIIIGHIGBDGIICGBAGDEHE;EGIEH=4?>BB;AC9A -@HWI-ST575:165:C1P5BACXX:1:1101:4670:2972 -CGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG -+ -FHHHHHJJJJJJIJIJJJIJEGIJJJAHIIJJJIGGFHCFFFGGGHFFF?CBBDDDDDDDDDBDDBDD<>>ACC(0 -@HWI-ST575:165:C1P5BACXX:1:1101:4779:2823 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHHIJJJJIJJJIJJJJJJJIJJJIJIJJJJIJJJHHHGGHFFFFEDDD>BD@BDD -@HWI-ST575:165:C1P5BACXX:1:1101:4804:2998 -GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTAC -+ -FHGHHHJJJJJJJJJJGIEIJIIJJJJJIJJJJJIIJJJIJIJIIGHIJHHFFCEEE?@:?BCDD -@HWI-ST575:165:C1P5BACXX:1:1101:5202:2792 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FHHHGHGEIIIGGIGFGDFGEHHEHGGIJGIII@CGEEHHHFDB?DEEDB?B88?ACCDD@ -@HWI-ST575:165:C1P5BACXX:1:1101:6392:2828 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHFFIIJJIJJJHIIJIIIJJIJJJIIJJJJJJIJJJJIJJJHHHHFFDDDD?BDD -@HWI-ST575:165:C1P5BACXX:1:1101:7180:2991 -TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -DHGFHHJJJJIIJJJHJJIIIJJJIJBHJJIIJJJJDIIJIIJJIHHHEFDDDDD?BDDDDDDDDB -@HWI-ST575:165:C1P5BACXX:1:1101:7419:2920 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT -+ -FHHHHHIJJJJIJJIIIIJIIJJIJIJJJIIIJGIJJIJIIJIIGHIHHHFDDD@BDDBD?BBDDDBBACBCD<82 -@HWI-ST575:165:C1P5BACXX:1:1101:7772:2979 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGAAAAAAAGCCTACGT -+ -FHHDHHHJJJJJJJJJJJJJJIJJJJJJIIGJJIIJJJJJJIFGIIHFGHFDDDBDDDDDDDCDBBD;ABBD?CACBA -@HWI-ST575:165:C1P5BACXX:1:1101:9990:2821 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHGHJJJJJJJJHIJIIJIJJIJIJIHIJJJJJJJJJIGGIIIJGHGFFDDDDDD(< -@HWI-ST575:165:C1P5BACXX:1:1101:10328:2966 -GTGCCTAGCGAAGTCATAAGCTAGGGTAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG -+ -FGHHGHJJJJJJJHHIIJIIJJJIIJBFHGHIJJGIGGIGIJJJJJHGFFDDEEDD=@BDDCB -@HWI-ST575:165:C1P5BACXX:1:1101:11508:2796 -GTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJJJJJIJJJIIIJIJIJJJIJGIJJJHIIJIIIJIJBEHHFDDED(;@@=;9=8((4:?>8 -@HWI-ST575:165:C1P5BACXX:1:1101:12044:2834 -TTATCCTTGAAAGTGCCATAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGAC -+ -FHHHHHJJJJJJJHIJJIJJJJJIJJJJJIJJJBFHGIJJJJJIGIIJJJHHJE==@BHHHBDFFFDDDDDB; -@HWI-ST575:165:C1P5BACXX:1:1101:12169:2963 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHIJJJIJJJJGIJGGJGGGIIJEGHGHHFBDFFEEEEDEDDDDCDDDDDDBDCCD -@HWI-ST575:165:C1P5BACXX:1:1101:13168:2760 -TTGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJJJJJJJJJJIIIIJIJJJJJJIJJCGBFGIJJIJIGGGHIIIHHDCDDBDDB -@HWI-ST575:165:C1P5BACXX:1:1101:13484:2768 -CCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATACTT -+ -DHHHHHHIIDIGIIIGIIJJIJJGIJIJBGDHIGHIHIIGJHHHHDDFFDED@>A>BDD -@HWI-ST575:165:C1P5BACXX:1:1101:13399:2774 -TAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG -+ -FHHHHHJJJJIJJJJIGJJJIFIJJJIJJJJJJGFGHJJIHHHHDDDFC@CEEACCCC>ACCDDDD@CCCD -@HWI-ST575:165:C1P5BACXX:1:1101:13467:2781 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FGGHGDHIGIIICHGBHIIJIIIEGJIJFHIJJIIJIHHIHIFIGGIHBHFDD@7ACCDDB2A -@HWI-ST575:165:C1P5BACXX:1:1101:13716:2837 -GATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAA -+ -FGHHHHJJJIIJIIJJIIJIIJJJGIJJJJJJHGJJJJIJIFIHHGHGGGIJIIGHICHGGACC?E -@HWI-ST575:165:C1P5BACXX:1:1101:14400:2843 -AGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAG -+ -FHHHHHJJJJJJIIIJIIJJIJGGJJJJJJJJJIGGGGIJHHHHB@DDFEEEEEECC@C -@HWI-ST575:165:C1P5BACXX:1:1101:14816:2802 -TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FHHHHHJJJJJJIJJJIIJJJIJHJJJJJJJJJJIJJIIJJJJGIJHHHHFFFFEDC;=;@CDD -@HWI-ST575:165:C1P5BACXX:1:1101:15055:2926 -GTCGTGCCTAGCGAAGTTATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -AFDAD@GH@B@?BDHGGI8=B4=@2@C62CB<<<9>=?; -@HWI-ST575:165:C1P5BACXX:1:1101:17639:2908 -TGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG -+ -FHHHHHIJJJJIJCIHGIGEIGIHIIJJJJHGIJIIJJJIHHHHEHHFEEFFFCEEE;@?BCCDDD -@HWI-ST575:165:C1P5BACXX:1:1101:20319:2983 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT -+ -FHDFFHJFGHHIGIIIIIJIIGHGIIIJJJIJJIJJJJIJIIJJJJIJJJHFFC?ACE;@B?BCDDDDDDDDB>88 -@HWI-ST575:165:C1P5BACXX:1:1101:20783:2769 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHFHH@GIJIFIGJEGGHIJGEGHEGGGEHHHFFFEEEDEEEDDDDDDDDDDDDDDA9? -@HWI-ST575:165:C1P5BACXX:1:1101:1673:3017 -GCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTA -+ -?FHFD?EGHHE?BFHG9AFDBHEHHE>;)?AH@GG9EI@2.;??==?<@AC;;1(;(5 -@HWI-ST575:165:C1P5BACXX:1:1101:2153:3048 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCA -+ -DABE>BF*90?@8?7 -@HWI-ST575:165:C1P5BACXX:1:1101:4715:3184 -AGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG -+ -FHHGHGBHJFGHGGJIGHCHGDHGIICHGIIIJIFFDGGHHEIIIJGIJGGHCEBDEFF -@HWI-ST575:165:C1P5BACXX:1:1101:4796:3234 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FGFHGFFGIJIJIIGAGFHGGIHGGEEG;DFIJIGHJJIIGHIHIIDHEEF -@HWI-ST575:165:C1P5BACXX:1:1101:7098:3130 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJJJJJJJJJGGIIIJIJJJJIIJJJIJJJJJJIIIIIHGHHFDDDD?BDDBB;?BDDDDB -@HWI-ST575:165:C1P5BACXX:1:1101:7842:3018 -TAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCG -+ -FGHGHFGHJJJIJIGHJJJJIIIIIIJJJJJIJJGIEFGGGIGIC9?BGGFHA=;EH>BDDDB -@HWI-ST575:165:C1P5BACXX:1:1101:8316:3056 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHFHHIJJJIJJJJIJJBFFFIJFIJIIJJJIJIFIIIGIJIJJGHHHHFDDDD@BCCADD8AACDDD -@HWI-ST575:165:C1P5BACXX:1:1101:8582:3236 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHAHHHHHGJJJEHFHGFFDHGGIIIIEGHIIJGGIDGHJGGIIIHGHHEFD>CDD1? -@HWI-ST575:165:C1P5BACXX:1:1101:14033:3072 -TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATAT -+ -D?@8CFGFEGBFHEHFHIIIHFHGICEEHIIFHHHIIGHHIIIHGHEFFFCC??A?A8?BBC>CBCB9?C15885+ -@HWI-ST575:165:C1P5BACXX:1:1101:14598:3012 -CCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTC -+ -FFHFFHFEEHDIGG;CCCAFHBFC9CBGCAHG@E6@@1BBCEG;=E;???@CC -@HWI-ST575:165:C1P5BACXX:1:1101:16490:3172 -GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJIJJJJGIJIJIGIJIIIIJJJJJJJIJJJJJGIJJHHAHIHHFDDADDC==@9?CDDBB -@HWI-ST575:165:C1P5BACXX:1:1101:16810:3009 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG -+ -FHHHHHJIJJJJJIHIJCGIIJJIJIJJJJJJJIJJJJJJGGIIIHHHHFDDDD@DD<2=;<<>A -@HWI-ST575:165:C1P5BACXX:1:1101:11999:3288 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCTGGAAAA -+ -FHHFHHHIIIGDIIJEIGDEIEGIGGIGIGJJFG=FHIJFGHIJJJEEHHFD/;@=C>A -@HWI-ST575:165:C1P5BACXX:1:1101:12147:3289 -TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FHHHHHJJJJJJJJJJHIJJJFIIJJIJJJJIJIGIIGJJJJJHHFHFFFFEEDCDDDDDDDCB -@HWI-ST575:165:C1P5BACXX:1:1101:12666:3312 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTA -+ -FGFHFHJJJIJJJJJJIIJIHIIHIIJJJJJIJJIIJJJJIJEIIGIJJHHBDD?BCDBDB5?CD -@HWI-ST575:165:C1P5BACXX:1:1101:12701:3456 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG -+ -FHDHHHIIIJJJIIIJJGGGGGIGIJJJIJEHIGHIIIIIIJIJJFGHHFFDDB=ACDDDD?BDCC@ -@HWI-ST575:165:C1P5BACXX:1:1101:13062:3317 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -DFC@DF<@?CGGEH:DDBFHGIIG@DHGGBFHIIIIIIIGGHHEGIEHFDFC?;;;? -@HWI-ST575:165:C1P5BACXX:1:1101:13545:3337 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJIIJJIJJJJGHIIGIHIJGIGIIIJJEIJJIIJJJJHJIJHGFFDD@BDDB -@HWI-ST575:165:C1P5BACXX:1:1101:14345:3429 -CTGGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FHHHHHJJJJJJIGJHIGGIHHIJJJJJIJJIJJJJHIJJJIJJGJJJJJJHHFFDD?A? -@HWI-ST575:165:C1P5BACXX:1:1101:14585:3422 -AGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG -+ -FHHHHGIJJIJIIIIJJIJJJJIEGHJJIJJJIJIJJIJJIIHIIJIJIIJJJHHHHHF -@HWI-ST575:165:C1P5BACXX:1:1101:16628:3381 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJIJJJJJJEGIGIGIIJIJJIJJJJIJIJJJJFIIIIJJJHGHFFDDDBBA<9=69ACDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:1080:3578 -GGTCNTGCCTAGCGAAGTCANAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGANAAAANCCTACGTC -+ -FGFH#2>EHIHIIHGHHHII#0?DHGIIIIIIGHIBHGCG>EIIJF@ADD=ABDB#,5??#,5<AA -@HWI-ST575:165:C1P5BACXX:1:1101:1525:3749 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTGGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -DHFFFHHHJHGGHIHGHHIIHIEEIJIIGHHCHHIEEE)?ADDDDEECDB?B=??ACCBBBDDDDC@B -@HWI-ST575:165:C1P5BACXX:1:1101:2431:3630 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FHGCFHHIIJJIIIJIGH?FHGIJGIGH>FFAD@CCCEEEEHFFBFAC@ADDD?D@BD -@HWI-ST575:165:C1P5BACXX:1:1101:2802:3618 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHGHJJJJIJIJIIIHIGIGIIIIJIIJIIJJJIIJJIGIGIIJJJGJHGFDBEEDDDBDDDDDBB -@HWI-ST575:165:C1P5BACXX:1:1101:3064:3520 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHFFIJJIIIIIGJEHIIJEIFHIJHIGIHGHJIJJ;>CCCCD;55;:3 -@HWI-ST575:165:C1P5BACXX:1:1101:4225:3617 -GAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG -+ -DDBHHHIGEGEAHIGIJJIIIHFHHGGGFHGGGA@;5AEEAADDBBCCC@CCECC>>>C@@C:C9<:A:@8 -@HWI-ST575:165:C1P5BACXX:1:1101:4165:3628 -TGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FHHHHGJJJJJJJIIJJ1CFHIJGGJJIJIJIJIGIJJIJJJJJJJIJHIGEFEBCE3>B -@HWI-ST575:165:C1P5BACXX:1:1101:4951:3655 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGG -+ -DDHDFFEGHIDHEHFEG;FIEHGE@GGIEHIGGI3CGEGG>9=CEEEH>>B>;@ -@HWI-ST575:165:C1P5BACXX:1:1101:4878:3725 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHHIJJJJJJJJHIJJJIIJIJJJJJIJJJJHIGIIJJIIIJJIIJJJHHDDDEED -@HWI-ST575:165:C1P5BACXX:1:1101:5530:3596 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FGDFHHIJJJGIIIJHEHIIIJJJJIIIJJJJJJJJJJIGJIIIJJJIIHBD<AFCCHIHHB -@HWI-ST575:165:C1P5BACXX:1:1101:9213:3506 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACA -+ -FHFHHHIGGIIJIIDHGHIIGIIIFIGFEDGGIIIIJIIJIIIJADGHGGFE>C@BC(; -@HWI-ST575:165:C1P5BACXX:1:1101:10414:3701 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHFFHGIIJJJJJJHIJDFIHIGGIJGHIJIIIHHJIIIIIGHIIJHEHIHHFD?A(=ADDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:10901:3561 -TCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCA -+ -FHHHHHJJJJIJIJFFGCIHIGGIJIAEGHF@GHIJIGGGGGFGBHBDADBDDBBD@DDDBBBDA -@HWI-ST575:165:C1P5BACXX:1:1101:10915:3580 -AAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC -+ -FHHGHHIHIJJIIJJIJJIJJFHIJJJJIJIGGHIIJIJJJIJIHHHHGHFFFFFEC?BDDD -@HWI-ST575:165:C1P5BACXX:1:1101:11184:3661 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -BC</'31;; -@HWI-ST575:165:C1P5BACXX:1:1101:11415:3524 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHGHHHGIJIJIJJJHGIHIGIIJIGIGIJIJGIHIGHIGIIIJIJHHHFFDCBD?AC?B -@HWI-ST575:165:C1P5BACXX:1:1101:12086:3581 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJJJJJJJJJIIJIJJHJJJJJJJJJJEHJJIIGJJJJJHHHFFDDB?@>CB -@HWI-ST575:165:C1P5BACXX:1:1101:12420:3632 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTAGAGGCTGACGGCAG -+ -FD;FDFEHIIII@GFHI;GGDHGB@FEHIIFEHHIIEEGA4@FFC;CHEDBB -@HWI-ST575:165:C1P5BACXX:1:1101:13315:3747 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCT -+ -ADADD<@+GIHHFHGDFFFFECDDD?BB8?(+ -@HWI-ST575:165:C1P5BACXX:1:1101:14952:3691 -CCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGAC -+ -FFHHHHJIJGHGIIJJJFHHJGHEHHIIJJJGEGGDGGAG?FHGGIGIIJIIJJIIH=EDFCDCDD>?B -@HWI-ST575:165:C1P5BACXX:1:1101:15058:3596 -CGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG -+ -FHHHHHJIIJJJJJIGJJJJJJJJGIJJJJJFGJJJIJDHHHHHHFDEFFFEEEEEEBCDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:17467:3686 -TTAGAGGCTGACGGCAGGAAAAAATCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCA -+ -FHHHHHJJJIIIIIJJJIEGIIIJGHIJGJJIGEIGIIIJJHHHHGFFFBDCAAEEC>@CCCCCDD -@HWI-ST575:165:C1P5BACXX:1:1101:17669:3607 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG -+ -FHFHHHJJJJJJIIHIJFGIHIJGJJJJJJJJJIJJJJIHGHHFFFFFDDDDDD@BC99@78ACDCD -@HWI-ST575:165:C1P5BACXX:1:1101:18356:3623 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJIIJJJJJJJJGHGGIJHJJJJJJJIJIJJJJJJIIFIJJIIHHFFDDBDC=A??ABCDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:18913:3633 -ATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG -+ -FHHHFHIJJJJJJJHIIJJJJJJJJJIIJJJJJJJJHHFFFDDDDDDDD/CCCDAC9< -@HWI-ST575:165:C1P5BACXX:1:1101:1773:3939 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -DFH@FHHIIBHBGIIDFCDCBD8BDDDACDDB@ -@HWI-ST575:165:C1P5BACXX:1:1101:4430:3860 -CATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FHHHFGJIJJIGIJJCGIIIHJIIGIIIHIJIFJ@DCGHGIJJ9EEEEEFFFFDDDDDBDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6400:3801 -AGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGAC -+ -FHHHHHJJJJHIJJJJIJIJJJIJJHHIIJJJJHIJIJJIGIGIIIIJJJHAECHFFFDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6719:3898 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHGFHHFIIIJIIJJFHJHIJJJIJJJJGEIJJJJGHIIGGICGHHHHFFFDDDD@DCC -@HWI-ST575:165:C1P5BACXX:1:1101:8167:3757 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -DF;CDFFGFFEHFAEGH;?DFBF@EEGEAHEIIFABGEIG;>CGG>EEEBDC3;,;;A -@HWI-ST575:165:C1P5BACXX:1:1101:9714:3894 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACAAGCCTACGTCTTCGGAT -+ -DHFHHFHIIBEGHIGHHAGGFGGEHIIJJGEHJGGIJJGIGIECEHEEEFF8@@B?C(59998@CDBDDCCCACCC -@HWI-ST575:165:C1P5BACXX:1:1101:12038:3902 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHFHIIJJJIIJJFHHGHGGIGIJIGHIIIGIIIIIIIIIIFGIIHJIIHGFDAEEDB -@HWI-ST575:165:C1P5BACXX:1:1101:12700:3779 -GGTCGTGCCTAGTGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG -+ -FHHHHHJJJJJJHGIJJIJIIJJIJJJJJJJJJIJIJJJIGJJIJJIIGJJGFFDCEECDDBBDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:12790:3907 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -DHD?FF@FGIGBFFEHGBFB@BBCCC@A -@HWI-ST575:165:C1P5BACXX:1:1101:15156:3801 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHDHHIJJJJJJIJJJFHJIGIIIJJIJCHIJJIJJJJJJJHHHHFFFFDDDDD?BAC -@HWI-ST575:165:C1P5BACXX:1:1101:16559:3892 -TGCCAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC -+ -DDCDHF:ECFGBHIEEEGFBFCB?DFFDD:??BFHDD=FAFEGB@GF;?>?=?D<>B@>=?&9<<< -@HWI-ST575:165:C1P5BACXX:1:1101:17255:3956 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHFHHIJJJJJJIJJJJHGIIIIIIJJJJIIJJGIJJJJIGJJJJJFIHHFFDD??35 -@HWI-ST575:165:C1P5BACXX:1:1101:17500:3790 -TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -DHDHHDHIIJIIHGI;FIEIGIIJIJJJJIJG@HIJGIIIJFHHGFFCDDDD@ACCD7@B8CCABA -@HWI-ST575:165:C1P5BACXX:1:1101:18440:3926 -CCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACT -+ -FHHHHHJJJJJJIIJJJJJJJJJJDGHJJJJIIJIJHGJJJJIJJJIJJJJJIJIJIJJ -@HWI-ST575:165:C1P5BACXX:1:1101:19111:3987 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHAFHHIIIJJJJIGIJJJGIAGGGHIJIIJGIGJJJIJIJJIIHHHGFDDDDD??CD -@HWI-ST575:165:C1P5BACXX:1:1101:19557:3888 -TTGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FHHHFDEGIGIGIJGIIDGGG -@HWI-ST575:165:C1P5BACXX:1:1101:19920:3801 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT -+ -FHHHHHJIJJJJIJHIJGIIIEHGIJIJJJJJJIIIJJJJIIJJIIHHHFFDDDD@CC5;@??CCCBDADDCDB88 -@HWI-ST575:165:C1P5BACXX:1:1101:1580:4241 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT -+ -FHFHGHIJGJIIHIBGGHDGGEHGIJEHIJGGHHGGIIIIHIDIJIGHHHFCDB??CCDD?EECDAB?CBB -@HWI-ST575:165:C1P5BACXX:1:1101:4437:4204 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FGHHHHIIJJJJJIIIJJJIJJJGGJJJIGIJJJBFHIJJIIJJIJJHIIIHC@==>?( -@HWI-ST575:165:C1P5BACXX:1:1101:7890:4076 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTTACGGCAGGAA -+ -FGFHHHJJJJJJEHIGGHIIICHGCFGHHGHGIBGCGHIGIJDGHIHEEEFBAB7= -@HWI-ST575:165:C1P5BACXX:1:1101:8052:4159 -TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -DFFFFBEFEHDA?GID?9?BDD?;C<=8BCFHIH@=DAEBBA:5??= -@HWI-ST575:165:C1P5BACXX:1:1101:8080:4229 -TTTCGGCGGATCCAAATCGG -+ -DHFDHFFEHGFGGEHIIIHG -@HWI-ST575:165:C1P5BACXX:1:1101:8526:4228 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJIJJJJJJJJIIIGGIJIJIJJJIIIJIJIJJJJJJIGHIIJHHFFDDBDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:8765:4049 -TAATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FHHHGHJJJJJJJJJJGIIJJIIIIJJJJJJJJJJJIFIGJGHGEHHHFEFDFDCA?1?BDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9822:4042 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHFHHJJJIJJJIJJJJIIJIGIIJJJJIJJJIJIIJJJIJJJIIIIGIJHHFDAC3AB -@HWI-ST575:165:C1P5BACXX:1:1101:10184:4066 -GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJJJJIJJJJJJIIJIIJJIIJJIJJJJIJJIIJJJGECHHFFDDDBBCD9 -@HWI-ST575:165:C1P5BACXX:1:1101:11794:4113 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAGCCTACGT -+ -FHHHHHIJIJJJGHHIIHGGHGIIJJJJJIJJIGIGJJJJIIJJJJDGHFFFDDDBCDD?2?BB?@DB -@HWI-ST575:165:C1P5BACXX:1:1101:15267:4200 -ACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCG -+ -FFHHHHIJIJIIIIJIGIJJJFFGHGHFHIJIIIFJJGGIGHHIIHGHFEFD>BBD?BBBDDDDDDDBDDDDA::+ -@HWI-ST575:165:C1P5BACXX:1:1101:17040:4246 -GAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAAC -+ -DDFFHBGGHIGGIIIIIGIGHHIHIHHIGCHIC=@ -@HWI-ST575:165:C1P5BACXX:1:1101:17659:4106 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT -+ -FHGDHDFIIIIHCHHEG6BHHIIIIGGIIHIIG;;@GIIIHIDCEIHHEDDDDD=?C?<=@?A@ACBDACDDDD@? -@HWI-ST575:165:C1P5BACXX:1:1101:17880:4233 -GGGTCGTGCCTAGCTAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGG -+ -FHHFFHHHAEDGBCFEGIGBBBC -@HWI-ST575:165:C1P5BACXX:1:1101:19348:4134 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHDHHHJJIJJJJIIJIIJIIBGBEFFEGEIGGHIJJJJJIJIIIIIJHHF:BABBDC -@HWI-ST575:165:C1P5BACXX:1:1101:1929:4262 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJJJJJJIJJIJIIIJHJIJJIJJJJJHHHHHHGFFFFEDEDBDDDDBBDDDDD?BDCDBD -@HWI-ST575:165:C1P5BACXX:1:1101:3262:4389 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA -+ -??FDFFGD>FGHHBGCFHEGCH9@D@ACEBEE>FGIGFFB:B==?A -@HWI-ST575:165:C1P5BACXX:1:1101:7054:4268 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHFHHGIJJIJJJJJIJGIIGEIGIIJJIJJJJJJJIGGGGIIIIGHHDDCCDBBDCC -@HWI-ST575:165:C1P5BACXX:1:1101:7021:4304 -ACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG -+ -FHHDGHIJJJIJJJIIJGIHIJJJJHHIJJIJGIIJJJJCCEAHCHB?CFC@CEEDEEDDDDDB -@HWI-ST575:165:C1P5BACXX:1:1101:7330:4272 -TTTAAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCT -+ -FFHHHHJIFHIHJIIJHHHIJGHJIIIIGIHJJGDHICG?FHEIIGIGIGIDGGEEECCECCDD -@HWI-ST575:165:C1P5BACXX:1:1101:7985:4412 -TTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGTGAGAGTGGAACGCGG -+ -FHHHHHJJJJJJJIJJJJJJJIJJJJJJJJIGIFIJIJIJJJJJJGIHIIJIJEHIGGH -@HWI-ST575:165:C1P5BACXX:1:1101:9154:4399 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGG -+ -DAA0CDHE?H@HGGI@EC -@HWI-ST575:165:C1P5BACXX:1:1101:9397:4390 -TTGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FGGHFHJIJJJIIJJJJJJIIJIJJJJJJJJJJJJIIJIJJJJJBEGGIIIHHFFDD@CC -@HWI-ST575:165:C1P5BACXX:1:1101:9626:4444 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHGHHHJJJJJJJJJIJJIIIGIIIJJJIJJJJJIJJJJJJJJJGHHHHFFDDDDB@>CDDBBBDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9920:4375 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAG -+ -FHHHHHIJJJJJJJIJIGHIIIJIJIJIIJJJJCGGIIIJIIJIJIJJHGF>CDD@C(;@? -@HWI-ST575:165:C1P5BACXX:1:1101:9928:4484 -GCGAAGTCATAAGCTATGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTC -+ -FGGGHHHHGIHIIJJJJJJIJJJJIIGGIGEHJJEIJIIHIGFFFCECDB?ACCDBDD -@HWI-ST575:165:C1P5BACXX:1:1101:10347:4290 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT -+ -FHHHHHJIJJJJJJHIIIJJIGJIIJJIGJJJJJJJIJGJJJIJJJHHHFFACDBBDCBDB@@CDD;A@CBDDB8< -@HWI-ST575:165:C1P5BACXX:1:1101:10607:4474 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJJIJJJIJJIJIIIJGIIJIJFIJJJIIJJJJJJJJJJJHFHF>ADBBD(< -@HWI-ST575:165:C1P5BACXX:1:1101:11109:4422 -GCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGA -+ -D?CFHBACEE@EEHBH?9AECEDDDBD;8 -@HWI-ST575:165:C1P5BACXX:1:1101:11803:4357 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHFIJJJIJIJIJIHHIIGJGIJJJIIIJJIIIJIIJJJIIGGFHHFDD@BBBCDBBB8A@A -@HWI-ST575:165:C1P5BACXX:1:1101:12622:4263 -AGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC -+ -FDFFHFIIIIAFHGEHEIGIIIGII9CFHGEBFEGHHGIGI@FH;)8CBHEED?D@?=B@BB -@HWI-ST575:165:C1P5BACXX:1:1101:13888:4376 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FGFHHHJGIJJJFGFHIHIIIJJGIIIIHIIJJGGIIIIBGGFIHHGHBDDDBD@BD?B -@HWI-ST575:165:C1P5BACXX:1:1101:14985:4327 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGCCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHFHHIJJJIIJIIIJIGIIIHIHJIIFGGJIJJJJJJIIFHGHEFFFDDDDDD?BC?5 -@HWI-ST575:165:C1P5BACXX:1:1101:15294:4416 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTA -+ -DFHCFF@HGGHIICCFHGDHHHEDBE@F@>=B;A=FHGGI -@HWI-ST575:165:C1P5BACXX:1:1101:16020:4286 -GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJIJJJJJJHIIJJJJJJIJIIJIJJJJJJJIJJIIIHIIHGFFDDBBCDB -@HWI-ST575:165:C1P5BACXX:1:1101:16371:4398 -CCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHGHJIJIGIHHIJJJJJIIIJIJDGHJJIGIFJIJJIIIJJJHHFFFF@BBBDDCDDD -@HWI-ST575:165:C1P5BACXX:1:1101:17919:4354 -ACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC -+ -BFHHFFIIIHIDHGJIIGEHIHE@FHF:CCCGGGIDFFBDGCA;@@AAEEBA:/=BACCCBB@B=@ -@HWI-ST575:165:C1P5BACXX:1:1101:20762:4415 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -DDADF8CBHEEGEGHAACGGGCFAEAHDGGIEG8BFHGEEHHGGAED;8;B -@HWI-ST575:165:C1P5BACXX:1:1101:21005:4492 -GGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FGHHGHIJIJJIJIJJIJDIIJJIIJJIJJJIIH>DGGHGHFEFCBC?BBDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:2208:4721 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGTATCCTTGAAAG -+ -FHFHHHGJIJGJIIIIJJJCICGGHIJGIIHIIGIGHIGIIHCHHIFCHFFDDB;>CCDEDCD3>> -@HWI-ST575:165:C1P5BACXX:1:1101:3594:4541 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHGHIJDGACEAEEGHEBFEGEGHEHHHHEHGHHGFHGGGGHCCC=BDF???15:9? -@HWI-ST575:165:C1P5BACXX:1:1101:4453:4680 -TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG -+ -DHFHFGJIJJIJIGIFHGEHGHIIJJJJJJJHGIJJIJJJJIIIHHHGFFDD?=>CDDDB@CDDD -@HWI-ST575:165:C1P5BACXX:1:1101:4574:4514 -TTGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAT -+ -DFHHBFHIJJHHIJIIGIIGGIJJIJJJJJGHJEAE?>BDDDDD@DDBBBBDDD@CD -@HWI-ST575:165:C1P5BACXX:1:1101:4512:4554 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHGFHJJJIIJJIHIIJIIIIGIJJJJFIJJIJIIJJJJIJIJJHGHFF@BDBBDCBD@BDDDDDB -@HWI-ST575:165:C1P5BACXX:1:1101:6091:4740 -GGGAAAAAAGCCTACGTCTTCGGATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC -+ -DHDHHH;E@C;CEFHHBGGIEDB=FDFGHIIHGDCHGGGIEHGICHCEEFGH;CDEF?BCBC -@HWI-ST575:165:C1P5BACXX:1:1101:7755:4724 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA -+ -DFA@DDHIBDGGGBG@A@?FHBHIGCFGE>GHHB@GHIIIE@;=@A?=CEE'=@@EEFFDC>/=8:BBA05 -@HWI-ST575:165:C1P5BACXX:1:1101:2354:4948 -GGTCGTTCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FDDFDHIGHGHIJJIGGHJGIIJGIJGHGHJJJGIGIIEGHEHFEHFBDDDDDDBDDDDDBBDDD?@A -@HWI-ST575:165:C1P5BACXX:1:1101:2921:4941 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FGFDFHHIIIGHGGIGEHHIJ@GHIIIJIGDIGIH>FGGGHEEEEEF>EDFDD?B@?C -@HWI-ST575:165:C1P5BACXX:1:1101:4132:4919 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FGHGHGIJJJJJEHGJJIIJHIIGCHIJIJJJJJIJIJJIJFEFFFFFEDD@BD@DD??D:DDDHIIDIIEAGEHAEEE -@HWI-ST575:165:C1P5BACXX:1:1101:6248:4986 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FGHFHHIJJJJJJJJGIJJJGIIJJJJJIJJJJJJJJIJEHEHHFFFFEDDDDDDB@@CDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6936:4836 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHHJJJJIGFHIJJJJIHGIIJJIIIGGIJJJJJJJIJIBEHIJHEFDCCB?BAC -@HWI-ST575:165:C1P5BACXX:1:1101:7709:4867 -AGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAA -+ -FHFHHHJJGJJJIJJJIJIJIGIII?FGIJJFHIIJIDIJJIGGAHIHIHEE>DADBBDDBBBDBB;<9@@BDD>A -@HWI-ST575:165:C1P5BACXX:1:1101:9439:4978 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHGHJJJJJIJJJJJJJIJJJIJJJIIJJJIJIJJJJIJIJHHHHFFFDDDDD@B?CDDDBDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9572:4907 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHFHHHJJJJIIIIJIIGIJIIJGGJIIJIJIJJIJJIIJFEIGIJJHGFF:BDBDA( -@HWI-ST575:165:C1P5BACXX:1:1101:9900:4892 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FGHFHHJJJIJIJJJJJJIIJGIIHIIIGIIIJJHGIJJJJIIJJJIIIJJHFFDDEACB -@HWI-ST575:165:C1P5BACXX:1:1101:9950:4989 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FGHHHHJJJJJJJJHHJIJIJGIHHJJJIJJJJJJJJJJJIJJIIHGIGHFBCDDDD( -@HWI-ST575:165:C1P5BACXX:1:1101:10290:4901 -GACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC -+ -FHHHFGGFHIGIHIIJGBFEGI?FHGHIGGBHGGIGIGHHIGHGGHHFEFDDDDDDBBD -@HWI-ST575:165:C1P5BACXX:1:1101:10794:4755 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FHHFHHIIIJJJJJJHIIGIJIJGIJDIIIIIGJHGIJIJJGGIJJJIJEFFFDDBD( -@HWI-ST575:165:C1P5BACXX:1:1101:11203:4970 -AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FGGFHHJJJJJJIJJJJJIJJJJJJJIIJJIIIIFHIJJJFHGIHIIGHIHHFFDDD3=ABB@BDCCBD -@HWI-ST575:165:C1P5BACXX:1:1101:11597:4816 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHFJJJJJJJJJJJIIIJJJJJJJJIJJJJJJJJJJIGIIIIJDGHGFFDDDDDCDDDBDDCCDB -@HWI-ST575:165:C1P5BACXX:1:1101:11814:4971 -CCGGTCGTGCCTATCGAAGTCATAAGCTAGTGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHGHFHJJJJJJIIGIIJJIIIJIJIJJJJJJJEGBFGIJJIEIJIIJIJJHH<>CA?CCD -@HWI-ST575:165:C1P5BACXX:1:1101:12815:4804 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FHFHGDHIIJIJGHIHGGHGIGJGIJJJIIIJIIIIIJJJJGJFII;DAHBECDDBC -@HWI-ST575:165:C1P5BACXX:1:1101:15064:4795 -TATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FHHHHHJJJIGIJJJGIIIJIJHIJIHIJJIJJJJIIIJIJHFFFECEDEDCDDDDBDBCCCD -@HWI-ST575:165:C1P5BACXX:1:1101:15338:4874 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FFCFHDHGHIJJIJBHIFHIIIJIJJEGFFEHIF9CFGHI@GGIGEFHHFDDDDB=C?B -@HWI-ST575:165:C1P5BACXX:1:1101:15739:4984 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -DAAACHDGGGIICG?FD?FGH@@GBCBB2.=CF=@D@CH;==@A==?A89?5< -@HWI-ST575:165:C1P5BACXX:1:1101:17879:4904 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA -+ -FHGHHGIJJIJJJJJJJIGGGIIJJJJIJIJJJJIJIJJIGHIIIJHHHHFFDBD?A -@HWI-ST575:165:C1P5BACXX:1:1101:18072:4949 -AGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGT -+ -FHGHHHJGIIJJIJIJJJJJIJIII@GHDDGGGIIIGIJIIIGEHHHF?BAEECDDD=B; -@HWI-ST575:165:C1P5BACXX:1:1101:18453:4916 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHFHHGGIIIIIHEFHFHGHIJIJIIAHIIIHHIIGIIJIJIJIJHHHEF@CB==?(9 -@HWI-ST575:165:C1P5BACXX:1:1101:19254:4780 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGGCTGACGGCAGGAAAAAA -+ -AFADFHGGFGIIEHAAG?GEHGEGBGDGEGGGIF>@CFFHIEEIHHHFFCBCB@?5?B -@HWI-ST575:165:C1P5BACXX:1:1101:19861:4798 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJIJJJJJIJJJIIIGHGIJIIIJJIJHJJJJIJIIJJIIIJHHFFDDBBDD=BBBBC@CDD -@HWI-ST575:165:C1P5BACXX:1:1101:19870:4937 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHHIGIIJJHIGGIFHIIEGGEHGHIIJJIIGIIJIJJIGIIIHHHFFDDDD?CD -@HWI-ST575:165:C1P5BACXX:1:1101:19796:4989 -AAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FGHHHHJJJJJJIJJJJJJJJJJJJJJJJJJJJIJJJJHFEFFFFDEEEDDDDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:20053:4972 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FGHHGHIJJJGIIIGHIHIJIADGHIHHGIJJJJJHIJIIHIJIIIIJHGFDCDBBDD????ACCCBD -@HWI-ST575:165:C1P5BACXX:1:1101:20782:4908 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHFHAHIIIIHICHE0DGGII>?DFDGGHIIEGAGIGIGIGIIBGE;CBDD@BDBB?C -@HWI-ST575:165:C1P5BACXX:1:1101:1189:5021 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTC -+ -AF22<@F@?CFFCF>EG7CDGCCBDAGHGFDFGB> -@HWI-ST575:165:C1P5BACXX:1:1101:1686:5115 -GGTCGTGCCTAGGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGCCAGGAAAAAAGCCTACGTCTTCGGATA -+ -FHHHHHJJJJFHIJIJJJJJJIJJIGHIJJJIIJIJJJJIIIJHGHIG'=EDF?>;AEDD@BCCDDDDDDDDB?<< -@HWI-ST575:165:C1P5BACXX:1:1101:2938:5127 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -?6CDHFIEDHHDHHGHHBHGGGIIGGGGEFHCECFEA>F;=@AEHD>DAEB<83=;? -@HWI-ST575:165:C1P5BACXX:1:1101:3405:5036 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJJIJIJJJJJJJJGIHIIIIIJIJJJJIIJJJJGCIIIJIGHFEDD?BDDBB=BBDCDBB -@HWI-ST575:165:C1P5BACXX:1:1101:7045:5134 -GGTCGTGCCTAGCGAAGTGATAAGCTAGGG -+ -D=DD8@@=EC<339:*?:? -@HWI-ST575:165:C1P5BACXX:1:1101:7123:5216 -TTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATG -+ -FHHBHHJJJJIJIIJEHFEGIJJIIIGIIHIGIJGIGDGGIJJJJJJJJIJJIJEHFHG -@HWI-ST575:165:C1P5BACXX:1:1101:8358:5145 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA -+ -FHGFDHEHIJHEHEFBDCAA@ADDCCDD@BDD@DCDDEDCCCDDDB2B?ABDDDDDDCBDB-22 -@HWI-ST575:165:C1P5BACXX:1:1101:10830:5146 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FHGHHHIJJIJGIIJEHIIIIGGGHGEIEHIJJIGHGIIJGHIIJHHGGFFDB@B@?( -@HWI-ST575:165:C1P5BACXX:1:1101:11032:5068 -TCTTGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGAC -+ -BDHHHHIIIIIGIAHHHGIIIHIII@HHCHIIIIGIDEHIIIGGIDFGDEIHAHFFFFBCCCB8? -@HWI-ST575:165:C1P5BACXX:1:1101:11565:5085 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHFHHHIEIGIIGGGGIHIGHGGEEGHGIIIJJHGGGJEHGHJIIHECHFDD?B=A?CD -@HWI-ST575:165:C1P5BACXX:1:1101:12209:5017 -AAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAT -+ -DBAFFFF2AEAB -@HWI-ST575:165:C1P5BACXX:1:1101:12070:5238 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATAT -+ -FHHHHHJJJJJJJJJJJIJJIIJIJJIJJJJJJJIJJJJJIJJJJJJHHFDDD9;?AB:9ACDDBABCBCDB?854 -@HWI-ST575:165:C1P5BACXX:1:1101:12614:5234 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGAAAAAA -+ -FHHHHHGIJJJIJIJIJJIJIIIJIIJIIJBHIJHIIJJIIJJJJHGFHEFDDB@CDDBD -@HWI-ST575:165:C1P5BACXX:1:1101:12934:5066 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJJJJJJIIJJJJHIJGJJIJGIJJJJIJJIJIIIJJJJIJHHFFD@?ACDBDDABDACB? -@HWI-ST575:165:C1P5BACXX:1:1101:12889:5153 -TAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FHHHHHJJJJJJJIIJJJIIIJJJJJJJJJJJJJBHIJJJJGIHFEHFFFFFDDDDDDDDB -@HWI-ST575:165:C1P5BACXX:1:1101:13006:5008 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTA -+ -:88<@CD48DDF>;C@F=@@@@E -@HWI-ST575:165:C1P5BACXX:1:1101:13701:5054 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FHHHHHJJJJJJJJJJJJJJJIJIJJJJIJJJIJIJJJJIJJJIIHGHGDDDDDBDDDBD -@HWI-ST575:165:C1P5BACXX:1:1101:13818:5190 -TTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTGGCGGATCCAAATCG -+ -DHHHHHJJJJIJJJCBG@HIHIIGIJGIJJJJGBFGFIJJJJJIFDDDDDDDDDDDBDDBDDD@CCCB -@HWI-ST575:165:C1P5BACXX:1:1101:14721:5211 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGG -+ -DFCDF1@AEFEF9CC@FBDBFFFF*9?F<=BDF8BF>FEFEEDCEEE9=B>'9> -@HWI-ST575:165:C1P5BACXX:1:1101:15180:5074 -GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJJJJJIJIIJJJJJJJJIJIJJIJJJJJJJJJJJJJIIGIJHHFFDDACB -@HWI-ST575:165:C1P5BACXX:1:1101:15733:5028 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHHJJJJJJJJJIJJGIJJJIIJIJIIJJJJJIIJJJIIGIJIJIIJJHFFDBE9A -@HWI-ST575:165:C1P5BACXX:1:1101:16783:5148 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHDHHFHJFJIJIIIGHBFHGJJIJJJJIGIIJJJJJJJJGIJJJIHHHFFDDD?AC(5 -@HWI-ST575:165:C1P5BACXX:1:1101:17093:5065 -GCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJJJIIBHGHIJJIJJJIGIGIIJJJJIIJJJJJJJJIEFFBEE9;8>ABDCDD? -@HWI-ST575:165:C1P5BACXX:1:1101:18177:5052 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FHHHGHJIJJIIIJHJJHJJJIJJIJJHIIJJJFHIIJJIIJJJJIJJHHFFDD?BCAAB -@HWI-ST575:165:C1P5BACXX:1:1101:18454:5064 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FAFHHHIIIJIJJIGJI?GFGIIGIGIHIGIJHIIJIIGHGIGIIIFHHFFDDD=BC -@HWI-ST575:165:C1P5BACXX:1:1101:19514:5155 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHFDHHIGIJGIIHDDHIIGIEFHIIGGGGIEHFHIIIJJHGIJJIIGIIHGFD?@EC -@HWI-ST575:165:C1P5BACXX:1:1101:21070:5172 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FGDFHHJIHGIIGH@FGDHGGEGHGHGGHGIGIGGGGHIGGIIIIDAGHE<99;??CC -@HWI-ST575:165:C1P5BACXX:1:1101:1152:5446 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -DDACF@EGHGGGHBGDHDDGAFDE??>C -@HWI-ST575:165:C1P5BACXX:1:1101:2332:5476 -ACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG -+ -FHHHHHIIJJJEHIJHJJIJIGIHIJJHHJIJHIJJIIJJDHDDCDBDDDDBDDBDDDDDD>ACCBB -@HWI-ST575:165:C1P5BACXX:1:1101:3549:5422 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJJJJJJJJIIIIJGIJIIJJGHGHHFFFFFEEEDCEDDD?CBDDBDDBACDDDDDBDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:3934:5255 -TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHGHGFIJJIJJIJJIJJJJIJIJIJJJJJJJIGIJIGJJJIIIIIJJHHFF7>CCEDDD@DDDBB -@HWI-ST575:165:C1P5BACXX:1:1101:3779:5257 -GTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHGHJJJJJJJJJJIJIJJJJIJJJJJJJJJJIIIHJJJJIIGFHFFDDECCBB@BBDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:3981:5396 -GTGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA -+ -DFDFHFEHBGECHBGGG@??B@?F>FBFFCHF7F/BFCHEGG4@FHH;EHD=49=;2 -@HWI-ST575:165:C1P5BACXX:1:1101:4352:5435 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHIJJJJJIJJJIJJJJJHHJJJJJIJIIIIIJJJJJJIIJIJJHHFEDDDDDDDDDBDCCCBD -@HWI-ST575:165:C1P5BACXX:1:1101:5757:5381 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -D?FFFF@GHIIGIGIIEIDFGIIIGIEGFB@DFIGHGIGIIGEGIIIHIG64'=>;,>3 -@HWI-ST575:165:C1P5BACXX:1:1101:7084:5290 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FHGFHHIJJJJIJIJJJJJJIHIJGIJJHGIJJJJJJJJJIJIIHHHGFFFDDDDBDD -@HWI-ST575:165:C1P5BACXX:1:1101:7550:5435 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FHHFHGIIIJIJJIJEHGHIIGJJIJJJC@GIJIJHIIJJJJHIFHHDFFDDDDD@AC -@HWI-ST575:165:C1P5BACXX:1:1101:8518:5454 -ATCCGCTTCGGCGGATCCAAATCGG -+ -D?FDHHJJJGBGIHIIIJJJJIIHG -@HWI-ST575:165:C1P5BACXX:1:1101:8740:5499 -GGGTCGTCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJJJJJJJJHIIHIJJJJGGIIJJJJJIJJJJJJJJHEEIFHFFDDBBDD2 -@HWI-ST575:165:C1P5BACXX:1:1101:8810:5337 -TGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAAC -+ -FHHHFHIJJJGJIJJIHHIJJJJICFGHIJIJJJIJJIJIIJJJJJICFHG7CFHIJBH -@HWI-ST575:165:C1P5BACXX:1:1101:10317:5279 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTTTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTTGGAT -+ -FHHHHHJJJJJJJJFFHIIJIJJIJJJJJJJJJJJJJJJJIJJJJHHHHFFBDDDDD3?BB=@F@=.=C??EC -@HWI-ST575:165:C1P5BACXX:1:1101:13619:5383 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FGHHHHJIJJIIIIGEHGIJJJJIJIJJIJIJJJIJJIJJIJHHHFFFE@BDDDBDD -@HWI-ST575:165:C1P5BACXX:1:1101:14813:5367 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FGFHHHJJJIIJJIHIIFHIIJGIJJJGHGIJJHIHIIJIJGHIIIJJIIHFFDACE9 -@HWI-ST575:165:C1P5BACXX:1:1101:16958:5440 -AAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACC -+ -FHHHGHIIJIJJJHIJJJJIIJJIIJJJGIGHJJJIFHIJIJJJJJGHIJJIHHHFFFFDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:17064:5269 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJJJJJJHJJIIJIJJJJJJJIIJJJIJIJJJJIJJJJJJIIIHHFDDEC3=@???CDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:18711:5374 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FHHHHHJIJJJJJJIIJHIIIJJIJJJJJJJJJIJJJJJIIJJJJIIJHHFFDDBAA=;= -@HWI-ST575:165:C1P5BACXX:1:1101:19795:5388 -AAAAGTGCCACAGGGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGG -+ -BCCCCD>CC>A@:>:CC -@HWI-ST575:165:C1P5BACXX:1:1101:3186:5524 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHGHHHIJIJJJJIJIJJJIIIJGIJJJFIIJJIJGIJIGIEGIHGEEABDDBBBBDDDDDBBCDDBB -@HWI-ST575:165:C1P5BACXX:1:1101:3099:5544 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FGHHHHJJJJGGIJJIJGHGGIIGIGIIGIIJJJIJJJJIIIIGHGHHHDDD/;BAC?B?;ABCCDDA -@HWI-ST575:165:C1P5BACXX:1:1101:4347:5721 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -DHFHAFGDHIIJIIGGGFHEGIJFIHGII?CEGHIIIIIJIIJCHHGGBEF'9@BBDCA -@HWI-ST575:165:C1P5BACXX:1:1101:5268:5661 -AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -DDD?D<>AFCFA9;@EFFE<@9'95? -@HWI-ST575:165:C1P5BACXX:1:1101:8832:5696 -TTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAA -+ -FHHHHHJJJJHIIEHJHJIJIIIJEGHIJJJJJJJJJIJIJIIIIJHJHFHDFCDBDDDDBDDDBBD9>9<>?:@A -@HWI-ST575:165:C1P5BACXX:1:1101:9211:5626 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGACAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJJJJJJJJJJJIHJIGIJJJJJIJJJJIJJJJJJIJIIJJHHFDDCDDC(= -@HWI-ST575:165:C1P5BACXX:1:1101:9028:5675 -TAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTGGATATGGC -+ -FHHHHHJJJJJJJJJJJJJJJJJJIJJJJJJJJIJJJJJJJJJJIBHEEEHFFFFEDEECDEDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9789:5670 -GGTCGTGCCTAGCGAAGTGATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJIIIJJIJJFFEHIIGIJGIIJJJJJIIJIJJIJGIGIHHHFF@BB=AD9A -@HWI-ST575:165:C1P5BACXX:1:1101:10507:5654 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -DFDDFHIGGGHI@FCGFBGGGICG>FDBBDCAFD>GHI;CGA2@E@=??B5(>( -@HWI-ST575:165:C1P5BACXX:1:1101:12148:5538 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHFHHGGJGIJJJJIIIGHGIIIIIJJIJJJJJADHIIIEIIHEFHFFEDDDDDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:12149:5686 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHGFHHGIJJIIJGIHGIDGIIJJIIIJJJJJFECGHIFFHHHFFFFEEDDDDDD?BCC -@HWI-ST575:165:C1P5BACXX:1:1101:12546:5747 -TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGACAAAA -+ -DHDDHFGEFHHGGIJCEF?@DCGIEGBDBHE?BFDHHIIGAGGFAFBEEFFD?(6;;? -@HWI-ST575:165:C1P5BACXX:1:1101:13615:5616 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJJJJJJJIJJJJIIIJIJJJIHGIJJIHIIJJGIJFHIHGFEEFDDDBDD?B -@HWI-ST575:165:C1P5BACXX:1:1101:13537:5735 -TTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAA -+ -FHHHHHJJJIJJJJJJJJGJJJJIGIIJGHIJJJJIIJIJHHGHHFFFFECECEEEAACD -@HWI-ST575:165:C1P5BACXX:1:1101:14584:5542 -TCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGA -+ -FHHHHHJJJJJJIJJJGJFFHIJJIEIJIIHIJJJJGIHIIIIJJJJGHGIJJJJHIEGHGEHEHE -@HWI-ST575:165:C1P5BACXX:1:1101:15724:5746 -TGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCG -+ -FGHHHHJJGHJJJJHHIIJIIIJJHIIIIHIIJJIJJIJHFFDDDDDDDDDDDDDDD:A@CCB -@HWI-ST575:165:C1P5BACXX:1:1101:17227:5625 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -DDCFFHEFGGGIEHGB?0??DBDB3BD@BDDBFBGIIICH@AFGIGE>AEDAB=B955? -@HWI-ST575:165:C1P5BACXX:1:1101:1924:5978 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHDFFFHFIIJJEHIJGBGHHGHFHHIGEIDHGFHGGGEHIHGJJHDHEFADDD??CCB -@HWI-ST575:165:C1P5BACXX:1:1101:4153:5778 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHGHHHJJJJJJJJJIIIJIGIJIIJJJIJJJJJJIJJJJJJIFGHHHFFDDDDDDD5<@B(8CDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:4024:5788 -TTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJBGHIDFHIEIGGIJJJ@HGJJIJGIJIJGFHIGIJJGIJIHFDBBCDDBDDDDCBB -@HWI-ST575:165:C1P5BACXX:1:1101:4463:5945 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGG -+ -AD@FHDFEHEHGGIIIE??BBG@DGC>CAFCHICHGI>CH;?=B?66;9' -@HWI-ST575:165:C1P5BACXX:1:1101:6188:5778 -TGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -DHHHHHJJIIJJGIJJJJJJJGIJIJJJHIIJIJIGJJJJJJJJEHHFDDEC@BDDABDA?@D -@HWI-ST575:165:C1P5BACXX:1:1101:6867:5946 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJJJJJJJJJHIGHIJIJJJJIIJJJJJJJJJJIJJJIJJJJJHFFDDEEDD -@HWI-ST575:165:C1P5BACXX:1:1101:7535:5814 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHDIJJJJJJJJJJJJJJJIGIJJJJJJJJIJJJIJFJGIJHHHHFFDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:7794:5903 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FGHDFHEHIIJJJJJIJIBHGIIIGIBHGHIIIIEHIIJJHHGEFFFFFCDDDDD?ACD2 -@HWI-ST575:165:C1P5BACXX:1:1101:9106:5832 -AGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAA -+ -FGFHHHIIDIHHIJGJJJJJJJIJJHIIGEHGIDIHIGIGGHHI@EHIHDHFFFBBBDDDBDBBDDDDDB@<<<:> -@HWI-ST575:165:C1P5BACXX:1:1101:9392:5986 -AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FFGFHHGIGIBGIEIE?FCFEGGFFHGEGFGGGEGFGFGHHEHHFIEDGGEAB/>=A(5 -@HWI-ST575:165:C1P5BACXX:1:1101:9549:5793 -TAAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACC -+ -DDDDDD2CEEEEEFAC:F?CEEEEEEIEE4CDFDEDDBDDDDEIDDDDEDDDD@@DBB?CB -@HWI-ST575:165:C1P5BACXX:1:1101:13983:5888 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHFHHHIFJJIJHHGHIGIJIIGIIJJJJIJGJEHIJJIJIJIJJJHHGFFBDDBDC5 -@HWI-ST575:165:C1P5BACXX:1:1101:13915:5939 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHFHHFHJJJJIIJIIJHIIIJJJJIJJIIIJJJJJIJJIIIFGIFEEEDDBDDBBD(5 -@HWI-ST575:165:C1P5BACXX:1:1101:14076:5802 -ACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCG -+ -FHHHHHJIJJIJIIJJJJJJJEGGIJJIJIIFHIJJJJJIJIHGHJHHHFFDDDDDDDDDDDDDDDDBDDDCC>@2 -@HWI-ST575:165:C1P5BACXX:1:1101:14165:5883 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHDFFDHEGIGIIGA@DFHGEIIGIEGIIDGJIEGIGGGEHGGHGIGHHHFDBDB?C?? -@HWI-ST575:165:C1P5BACXX:1:1101:14716:5991 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGTCAGGAAACA -+ -FHFHGHIJJJJJJJJIHDHGHIJIJJJJJJJJJGHIJJJJJJJIIHHHGFFFDEEDD(> -@HWI-ST575:165:C1P5BACXX:1:1101:15153:5829 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTCTAGAGGCTGACGGCAGGAAA -+ -DHHDHFIIGHGHI>?FDH6BDFGG@FBGIBFHBGGHGGCGIGEHHCEBCAC>9=?(9> -@HWI-ST575:165:C1P5BACXX:1:1101:15618:5813 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGG -+ -DDFDAFGIJEIIDIGHBFGGGIIHGIIIAFDHIHIEDHCGEHFGCHHHHDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:16278:5962 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGGCTTT -+ -DF8DFFDGE@EFFG:?D?DDAFIDFFEAECFIF':AEF -@HWI-ST575:165:C1P5BACXX:1:1101:17404:5768 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHGDHHGIIGEHGGJBHJIGIIJIIGIGIHJJGHAHIJJIGIEGGHHGHFFACDD7<:< -@HWI-ST575:165:C1P5BACXX:1:1101:18508:5770 -CGTGCCTAGCGAAGACATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHGHHHJIJJJIIJIJJJIIIJJIJJJJIHGIIJJJIJIJJJJAEHHHDDD?=ACBDDDDDDDBB -@HWI-ST575:165:C1P5BACXX:1:1101:18654:5893 -AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHHJIIJIJJJIIJIIJIJIIJJIJIIJIJJJJJJIJGGIIJJIHIJHEFD?>35?@@9ABBBBBB;A(<5<95DHIDHIBFGCDHFCHEHGGIIHJGIJGGJJFFHHHDE>AB@BBA3 -@HWI-ST575:165:C1P5BACXX:1:1101:4344:6153 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAACAAAGCCTACGTCTTCGGA -+ -FHHF)@GHIJJJIGIIJJJIBGIIIIIJJIJJGIIIIJJJIIJGEHHHHDDDCDDBD(,(5??ADDDDDDCDDDB< -@HWI-ST575:165:C1P5BACXX:1:1101:5212:6077 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -DF@FFFHJIIJJIIJII?FDHHIGIJGGGGIGGHHIIIJJGGIGHFHDDB<>BB??C< -@HWI-ST575:165:C1P5BACXX:1:1101:5854:6193 -AGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG -+ -FHHFFHEHIJJJJJJIJFHIJFIBHHAAC@CC25 -@HWI-ST575:165:C1P5BACXX:1:1101:6093:6246 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAACAAAGCCTAC -+ -FHHFHHIIEIGEIIIEBHFGBFBHIIJGIGIGHJHGHGGGHEGGGIJ9=ADDBA??B295<>?BAAD -@HWI-ST575:165:C1P5BACXX:1:1101:8105:6158 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FGFHFFHGIJJJIIGIIDFIGIEHHIGIGBFDHIGIIGGGHEGGGEEHHFD?@B7=( -@HWI-ST575:165:C1P5BACXX:1:1101:8253:6223 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGCAA -+ -DF@DFCFFBFFIBCDF:0??DFAGFI<8?FE;FFFE@F@CFE@EE>>CEDC/>:',5 -@HWI-ST575:165:C1P5BACXX:1:1101:8552:6031 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACTGCAGGA -+ -FGA@DFFEGHEH@FGDGGBBDGIGIGGJEGGGIGID=AGIJHHJJG?;CHEEFFF7 -@HWI-ST575:165:C1P5BACXX:1:1101:8575:6101 -TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -BDDDFHFHEGGGIIEHGGHHGCH@HGGGGBG?GG?@6CFH?B?CDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9569:6015 -ATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATC -+ -FHHHHHJJJJJIJJJJJJJIJIJJIIJJJJJJJJIIJJFIIIGH@GIIGIJGGIGIJHFFFFEEDDDD'38;@90) -@HWI-ST575:165:C1P5BACXX:1:1101:10905:6024 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -DHFADFEGDFEEEDGI@:?CFGDGD@FDFB?BF@?DFEFGB<3? -@HWI-ST575:165:C1P5BACXX:1:1101:21261:6248 -TATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGA -+ -FHHHGHIJJJJJBFHJIJIJJGIGFFGEIJJJJEHJJIIIIEG@FHIHIGIAGGDGGGGE -@HWI-ST575:165:C1P5BACXX:1:1101:1089:6262 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FH@FFDGIIIIIGHHFH?FGEGGIFG>GGHEHFBGHEFGFHIG=;AAHEFFBBB?ACCAB -@HWI-ST575:165:C1P5BACXX:1:1101:1439:6319 -TATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -2C?< -@HWI-ST575:165:C1P5BACXX:1:1101:1829:6451 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGATGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJIGHEFFHGEFGIIIIIJJIJJIJIJIJIIIJIJJJJJBHIJJGFF?@CCB@D??CC?=A -@HWI-ST575:165:C1P5BACXX:1:1101:2484:6444 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -DFFAFDFEFGGIIIGGBBFGIJEGIIDD8FGGE8==CH@@D=@E@AAEHEBB<;5;? -@HWI-ST575:165:C1P5BACXX:1:1101:3350:6364 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGC -+ -FHDFFHEDEEFGHIIGB0?A9BAFFAGGHE@AFHGAGE>AAEHH -@HWI-ST575:165:C1P5BACXX:1:1101:3699:6336 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGA -+ -DDDFHHEG;FHI>F?F@?CFGEEH>D@(@B/=>>8( -@HWI-ST575:165:C1P5BACXX:1:1101:3874:6280 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGATGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGA -+ -FHHHHHJJJJJJJJJJJJJJIIJJJIJJJHIJGIIHJJJJJJJIJJHHHHFFDDDDD(99@BDDDDDDDDDDDDD< -@HWI-ST575:165:C1P5BACXX:1:1101:4404:6415 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHFHHHJJJJJJJJGIJGIJIJJIJJGGGIJJJIJJJIJJIJJJJJ;EHHFCDD=BD39 -@HWI-ST575:165:C1P5BACXX:1:1101:5416:6451 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACA -+ -FHHGHFGHIJIJIJIIE@FFIIIIJIJGFIIJJIJJIJJJIGJJCHCEHBHFFDDBE(> -@HWI-ST575:165:C1P5BACXX:1:1101:7066:6455 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA -+ -FHHHHHJJIJJJJJIJJIJJIJIIJJJJJJJJJIJJJJJJIJJIIJIHHHFBDD@AC -@HWI-ST575:165:C1P5BACXX:1:1101:7488:6251 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT -+ -FHHHHHJJJJJJJJJJJHJIIJJIJJJJJJJJJIJJJJJJJIHHHFFFFDDBDDD?C??B>BDCDDDDBCDCDD?8 -@HWI-ST575:165:C1P5BACXX:1:1101:7486:6329 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHGHHHIIJJJIJIIJEHGHIIJJGGJJJJIJJJGIIJJJJGGIJIIGJJIHBEDA@C=B -@HWI-ST575:165:C1P5BACXX:1:1101:7643:6450 -ACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG -+ -FHHHFHHGGGHGIGJGBHGHIGHIIJEGHHEFFDB@D8A@CCCCBB -@HWI-ST575:165:C1P5BACXX:1:1101:8454:6487 -TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FHHFHHIIIJCHHGGHGGGHHFHFHEHJJ@FHIJIJICGHGHIGIJHEEFFDFFEDCDD@>CDD -@HWI-ST575:165:C1P5BACXX:1:1101:9137:6329 -AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHHHJJJJJJJIIJJIJJGIJIIIJJIFJJJIJIJJJJJJJJJJIJJHHFCD??;? -@HWI-ST575:165:C1P5BACXX:1:1101:9587:6277 -GCGGTCGTGCCTAGCGAAGTAATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHFHJHJGIJJJIJIIJFEHIGICHIIGGIFIJGIJIJJGHHDFFFFFDDD?D@?5>AB -@HWI-ST575:165:C1P5BACXX:1:1101:9536:6348 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHDHDHIIGIJJIJHIIEHIHGGHDGHEGHGGHHGIG@C=GIAHHGEHFHEDCB=55,9 -@HWI-ST575:165:C1P5BACXX:1:1101:10054:6289 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHHHHJJJIIIIJJIJJHIGHIGIJJJHIGIJGHGGIJI@HGGJJGGHFDDDDD;>,9 -@HWI-ST575:165:C1P5BACXX:1:1101:10173:6294 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FHHHHHJJJJJJJJJJIGGIHJJIJJGJJIJJJJJIJIJJIIFIJJIIJJHHFD;6;(;; -@HWI-ST575:165:C1P5BACXX:1:1101:10764:6333 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FHFFDDEGBFFHHIGGGHGHEHIEFGHIGBGIGEHHIIGHCCHHFFFDBCBBBB155(95 -@HWI-ST575:165:C1P5BACXX:1:1101:11047:6477 -GCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -BDFFFBGIGFFGFGHIEIIIG@HG@:GEDBHGIGG;5@;=@E;AEE@DFDCCC6A@CB -@HWI-ST575:165:C1P5BACXX:1:1101:11411:6372 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHFFFHJJIIGIIJIFHEFCHHGHGIGGIFFHHFFFFFFEEDEEDDDDDBBD@BD?ACC? -@HWI-ST575:165:C1P5BACXX:1:1101:11665:6448 -GGTCGTGACTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHFHHFIEIJHIHHHIIIHIHIJIIJHIJJIIIIIJJJIIIJIIGIHHHHFDDD@ACDDDDABC@<@? -@HWI-ST575:165:C1P5BACXX:1:1101:12774:6457 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FGH@FFDEHIGCHGHGHIFHHIGJEHIJJGIJGH@@DGGGIGHIBCEHCBBD>>@=(:?5 -@HWI-ST575:165:C1P5BACXX:1:1101:13106:6369 -AAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC -+ -FHHHHHJJJJJJIJGJJJJJIIJJJJJJJHIHIJJJJJJJJJJJJJJIJIJIHHHHHFFDE -@HWI-ST575:165:C1P5BACXX:1:1101:16704:6321 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHFHHIJJJIIJJJJIJGIJIJJJIJIJIJJJJJIJJJJJJJJJIJIIIHHFFDDEDDD -@HWI-ST575:165:C1P5BACXX:1:1101:16660:6380 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHFHHHJJJJJIHIGIIGGCHGIJIIIIJJJJJJIJJJJJEIJJJJIGIJHHFD5@CCB -@HWI-ST575:165:C1P5BACXX:1:1101:17964:6393 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA -+ -FHHFFHIBHGGEIHHFHIGGHHC>FBGIGIJI7F@DFHGIEGCHGEHGFFFDDDD5? -@HWI-ST575:165:C1P5BACXX:1:1101:18173:6282 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHFFHGHIJJJJJJHGJEHJIJJJIJJJJJJJIHIJJJJJIJJJJHGFFFDDDD=AACBDB1AEDC@C -@HWI-ST575:165:C1P5BACXX:1:1101:20043:6415 -ACGACGATCTTCCGATCTCGGACACAGACGG -+ -DDDAFF6DEFIIFIECG>FFI>FIFFEBE'5 -@HWI-ST575:165:C1P5BACXX:1:1101:1982:6500 -ACGACTCTCTTCCGATCTGGACACCGG -+ -FHHG?FHIEGIH@GEHIIIJJIJGCHH -@HWI-ST575:165:C1P5BACXX:1:1101:1934:6651 -TGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -DHFHHHIJJJJIGIIHIGIIIJJIJIJGIJIIJJJIJJJGIIFIIIFIJIHFFDCCDCDBBBBDCCBD -@HWI-ST575:165:C1P5BACXX:1:1101:3031:6587 -ATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FHHGHHJGHJJJJIJIJJJJIJJJJJJJJJJJGIGIIIIJIFHHHHFFFEEDCD:B?BDCCD -@HWI-ST575:165:C1P5BACXX:1:1101:3659:6632 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FHFCFFEHJIIIJIJGGIHHGIIJIGIJIAGH?HGIIJJEIGGEHHHFFFDDBBB?B:5(0 -@HWI-ST575:165:C1P5BACXX:1:1101:4271:6639 -GGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGA -+ -FGGHHHJIIJJJEIIIJJJBHGIIICHCFGIJE?GDH9FHHJJI)=8@FAFFGIEHGIH? -@HWI-ST575:165:C1P5BACXX:1:1101:5521:6516 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACAAGCCTACG -+ -FHFHHFIIJJIGEHHHBDGFIIIIJIGIIIJIFGIIJJJIGCHIIIEHH>BCDB?BC3?(,98C>CB -@HWI-ST575:165:C1P5BACXX:1:1101:6888:6616 -GTTAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGA -+ -DADFB<+C?GBH?GFGF8CDF -@HWI-ST575:165:C1P5BACXX:1:1101:15075:6709 -GGTCGTGCCTAGCGGAGTCATAAGCTAGGGCAGTCTTTAGAGG -+ -DFFFFDGBGB?ECF)0?0:9BGG<9B>3DF8BF.8@@F>@C>@ -@HWI-ST575:165:C1P5BACXX:1:1101:16741:6696 -GATCCGTTCGCATTGACACTGCTTATACGTGTAGACGG -+ -FHHGHHJIIJJGJJIIJJGIIJJJJJJJJJJJJJIJJJ -@HWI-ST575:165:C1P5BACXX:1:1101:16765:6674 -AGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG -+ -FHHHHHIIIJJJJJHIHIJJJJJJIJJJJJJHHHHFFDDDDDCDDDDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:19358:6651 -ACACGACGCTCTTCCGATCTATGATACGCGG -+ -DFFFFAA)@FFEC@F:?BD>?DF@FEECFAF -@HWI-ST575:165:C1P5BACXX:1:1101:19561:6627 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCA -+ -DDDDDCEDEIEC3C?D@:?DECDEDDDDBDDDA=BBDEEI>@>CDDCD8=?BDBB@859?C?@BBB@(9; -@HWI-ST575:165:C1P5BACXX:1:1101:1927:6913 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHHHHJIEGII@DAF>=BC58@@EFCFEEAEFF>@=@?'3;59>@?@ -@HWI-ST575:165:C1P5BACXX:1:1101:10199:6779 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA -+ -FHHHHHIJJJIJHGIJIHGGIGIIIJJJJJJJJIJIJJIJIJJJHHHFFFDDDD<5:,<= -@HWI-ST575:165:C1P5BACXX:1:1101:11749:6891 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FFFFFHJIJJIJIJHIJIJJIIJIGIIJJJJIJIIIJJJJIIGIJIJJHHFDDC?ACDB?@?ACDDBA -@HWI-ST575:165:C1P5BACXX:1:1101:12769:6834 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCCGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHJJJJJJJJHIIJJJIJJJIJJJJJJJJJJJJJJIIIJJJIIJHFDDDDDBDDDDD@BDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:13711:6990 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA -+ -FHHDHHGIGIIFIIIJJIIJIGJJEIJJJIJIGJIJIJJJJDGIIJJIIEHFDCDB?CD? -@HWI-ST575:165:C1P5BACXX:1:1101:14111:6893 -GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHHHHIJJJJJJIIJJHIIIJJJJJIIJJJJJJJJJJJJIJIJJJHHHFFDDD?BDDD6=A@CCDDB -@HWI-ST575:165:C1P5BACXX:1:1101:14440:6994 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA -+ -FHHFHHHIIJGIIIJHJJHIJIIJIJJJJJIJJGFHIJJJIGHIJHHFHFFDCDDBDDD -@HWI-ST575:165:C1P5BACXX:1:1101:15694:6869 -ATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAC -+ -DFFD??>FHICHGHHIIIIEDHHGHHIIIIHIGFHHBDECCCBBBBB?85@BCC(:< -@HWI-ST575:165:C1P5BACXX:1:1101:16093:6801 -TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAACAGCCTACGT -+ -FHHHHHJIIJJIJJJIJJJIJIGIJJJJJJIJJIIIJJIJIGHJJJJGIIHHFDDDB>A(=@FGGIEEEDFBAB;ACC< -@HWI-ST575:165:C1P5BACXX:1:1101:17036:6768 -GGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG -+ -FHHHGFGIIIIGIIHIGIJJIIJHHFGFFFFFDCDDDCCCDCBBDDDDDDDDCC3>AC@ACACBDCCCCB -@HWI-ST575:165:C1P5BACXX:1:1101:17542:6830 -GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT -+ -FHHFHHHIJJJJJJJIJJIJJIJFHIJJJJJIIIHJJJJIJIJIHHHHFEDD?BB?B -@HWI-ST575:165:C1P5BACXX:1:1101:1577:7179 -TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC -+ -FHGHGHIIIJJJIAHI@GIIH@HCHCEGGG?FGDBHH;BCHGIGAAEECEE9@DEDD?BDACCD -@HWI-ST575:165:C1P5BACXX:1:1101:2297:7148 -TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATGGC -+ -AFHHHHGIIIIIIIIIDGDHIGHGIIIIIIIIIIBFGBHIIIGHEHHGFFFDCAEABBD?BD diff -r fb76303acf4f -r 431aebd93843 rna_probing/test-data/reads2.fastq --- a/rna_probing/test-data/reads2.fastq Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,2684 +0,0 @@ -@HWI-ST575:165:C1P5BACXX:1:1101:1666:1132 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -HIJ0??FFCDB?FGDBAEHHBDDDB?@;8?==@8B87,4::4:(+4@< -@HWI-ST575:165:C1P5BACXX:1:1101:2239:1101 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACT -+ -JJJIGHIGBHHIJJIIGIJH6BDDBBDDBDDDDBBBDBD?CCBDDDDBCCDDDCCDCDCA:@A34(++4+4:: -@HWI-ST575:165:C1P5BACXX:1:1101:2455:1083 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTT -+ -GBGIIGGD@DGHGGGGIAEABBFBDB>B?@=:@BDB<8B9@C8-5<C>>AC4+++++4+44 -@HWI-ST575:165:C1P5BACXX:1:1101:3207:1238 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIHIJJHIIJJJFII@EHEEDFABDDDDDDDDDDDBBB>CDBDBDBDDDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:3405:1152 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT -+ -GG8:F6B*?3?B;@;;'54==46<>89'5=6,5-0&&0?(:+5;77-?? -@HWI-ST575:165:C1P5BACXX:1:1101:8268:1157 -CCGATTTGGATCCGCCGAGGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJJJJIIIJJGJIHHFDDDBBDDDDDDDDDBDDDDDDDDDDDBDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:8740:1185 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIIJIIJJJJJJJJIJJJHHFFDDDDDDD=BDDDDDDCDDDDDBDDDDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12414:1099 -CCGATTTGGATCCGCCGGAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJJJJJJIJJJJJIJJIJHHFDDDDDDDBDDBDBDBBBDDDCBBDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:14053:1112 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJJJJJIJIIIIJJJHHFFDDDDDDDDDDDDBBDDDDCDBDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:14219:1152 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJGIJJJJJJJIJJJJIIHHFFDDBDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDDDDDDEEE:(++4(444:AB -@HWI-ST575:165:C1P5BACXX:1:1101:15012:1211 -CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJIGIJIJJIJJIJJIJIIGHGFFDDDDBDDDDBD@@BDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:15774:1231 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA -+ -IJJHIJJIJJJJJIJJGIIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDCAACED4(+,++4 -@HWI-ST575:165:C1P5BACXX:1:1101:17047:1127 -CCGTCTTGGATCCGCCGAAGCGGA -+ -D@D0?)8*0*/0?86;@';'-=9A -@HWI-ST575:165:C1P5BACXX:1:1101:18461:1188 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC -+ -BC@?FEGGHDDGEGAC@BHHEFEBBBDBDDB'5<<<5:??CDD;BDD@?DDACDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:19911:1138 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJBHIJJJJJGIGIDHHGH>DFCD>BDDBDDDDDD7@BDCCBBDDDDCCDDCDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:1311:1500 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJIGIJJJJGIIJJJJJIIHHFFDDBDDDDDDBDDBBBD>ACB@BDDDCDDDDDCCDDDDDEEE:4:::4>4::>@ -@HWI-ST575:165:C1P5BACXX:1:1101:2110:1396 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -EHHF@GGG>GHFHIIGIEIBBBBC@?>;8=@BBBB@B8?3@@< -@HWI-ST575:165:C1P5BACXX:1:1101:5495:1254 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIJFIJGIIJJIIIIHIJHHFFCDDDDDDDDDDDDDDDDDCDDDDDDCEDDDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:5684:1314 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IIJJJIJGHEH*BGGIFHGGIGHHFFFDDDB;BBDBDBDCCCBDDDD?CCDDDCDDC -@HWI-ST575:165:C1P5BACXX:1:1101:6002:1450 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -FIIDHHGIFIIJEIICHGIEDFFDD@D@@DD'77B@BDBCDD8DDDDBDDCDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6163:1455 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJJIJJJJJJJJIJHHFFDDDDDDDDDDDDDDBDDCDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6370:1342 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT -+ -?;@:@?;DDD?8/8B;A4A:39353;;50:C()7A=;8283<395BB7::4+>44::8 -@HWI-ST575:165:C1P5BACXX:1:1101:20338:1414 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -GIG?@DFHFG>F9F@FHGGHFFFC@>>BBBBBDBBB07@CDCB7B@D?ACDCD -@HWI-ST575:165:C1P5BACXX:1:1101:1782:1682 -CCGATTTGGATCCGCCCAGCGGATCGGTCGAGG -+ -GGFH:8?AB=<9A8=8?BBD -@HWI-ST575:165:C1P5BACXX:1:1101:2733:1718 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTC -+ -HGGHGIGGGGHHCHIIIBHBEFFDDB8?<@BBB8BDDB@>@A@BDBBBDDCD?CC -@HWI-ST575:165:C1P5BACXX:1:1101:2796:1586 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJIJJIEHIJJJIJJIIJHHFFCDDBBDDDDDCDDDBD>CA8;BDBDDEDDDDDDDDCC@CDE:,+4,(444+:8 -@HWI-ST575:165:C1P5BACXX:1:1101:3075:1523 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIBHIGHIJJJJJIJAGAHFFDDDDDDDDDDDBBDDDCCDDBBBDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:4251:1515 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIHIJJJIJJJJJJJIJJJHHFDDDDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:4481:1718 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJIJJJJJJIJJIIJHHFFDBDDDDDD?BDD@7@DD:ADDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:4978:1615 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIJJJJJJJJIJJJJJJJHHFFDDDDDDDDDDDDDDDDDDDBDDDDBDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6935:1687 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJGAH?DBHGIGEHIGIEHEFEBB;@B?BBB:55798?AC:@BDB<@C@CCCCCCC -@HWI-ST575:165:C1P5BACXX:1:1101:7393:1637 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCCCGTTCCACT -+ -IHJJJIAHGIIGE@FGBEGHGFFDDDDDBDDDDBB:79@CCDCBBDDD?CDD -@HWI-ST575:165:C1P5BACXX:1:1101:7744:1585 -CCGACTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCC -+ -B8@@G61D@?*?.81;A/A=6B>:CDD4,+44 -@HWI-ST575:165:C1P5BACXX:1:1101:8452:1710 -CCGTCTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJJJJJJIJJGGIGFGFDDDDDDDDBDDDD87@CDDDDDDBBDEDDDDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:8575:1513 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -JIIGHJJJJIJJJIGJGGGHHFFDDDDDDDDDDBDB@BDDDCD@DDDBCDCDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:8610:1649 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJIIJJIJIIJIIFGIEHJJJIJHFEFDCDDBDDDBBBDCDDDB5;@B595B<:8?ACB77;B?CDC>ACCAC -@HWI-ST575:165:C1P5BACXX:1:1101:9782:1735 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJHIJJJGJJJIGIJEIJHHFFDDBBDDDD?BDBBDDDCDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:10892:1546 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTACTCTCAC -+ -HIIBBFHIIEHIDGIJIGEEEFDDDDDDDDDBDDDB@@BDDDDDDDDDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:10875:1631 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJIIGIJJJIJJJIJIIJIGHFFDDDDDDDDBDDDB:?ACCD -@HWI-ST575:165:C1P5BACXX:1:1101:17059:1656 -CCGATTTGGATCCGCCGAAGCGGATCGGAGAGGGGTTTACCGAGTTCCACTCTAAC -+ -JJJGIIJJJJIJJJJJJIIHHFFCDDDD@D@DDDDDBBCCCDDDB@@@CDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:17823:1711 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA -+ -JJJAHIIJIJJJJIIJJIIHHFFDDBDDDDD=BDDBAC?BBDDDDB@B@CDCDDDDD?DEDDCDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:20160:1653 -CCGAGAGTCCAGATCGGAAGAGC -+ -JJJJJGJFHIJJIDGGHGIGHGH -@HWI-ST575:165:C1P5BACXX:1:1101:20348:1677 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IGIFHIJJIBBDDBDDBDDDDDDDDDDD;BDDBDEACCCDDD -@HWI-ST575:165:C1P5BACXX:1:1101:1152:1999 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJFGJIJJJJJJJJJIJJHGFDACB@BDBDCDDDDDDD:?CC@@CD4++444>:44>8 -@HWI-ST575:165:C1P5BACXX:1:1101:1575:1782 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC -+ -?DD:FG7DB9>988,8;89B8778?(:><@BBDDGGE@>GBGGEGHFEGEFDD6>>?6?BD=05)579BC@@BD;;@DDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:5190:1986 -CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCACAC -+ -JJJIJGGDFCHIJCGIGGAAA?DDDBBDDDDDBDDD5:BCDCDBDDBDCDDDD3>AD -@HWI-ST575:165:C1P5BACXX:1:1101:7320:1866 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJGIJJIIJJJJJJIIGJJBHFEDDDDDDBDDDDDDDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:7508:1798 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJHIJJGJIIJJJJJIEGHHFFDDDDDDDDDDDDBDBDDDDDDDDDBCDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9647:1841 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGCGTTTACCGCGTTCCACTCTCAC -+ -JJIGHIJJJJIJJIJJIIJGGFFBBBDBDDDBDDD@DDDBCCDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:11078:1760 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJJJJJJJJIJJIJJHHFFDDDDDDDDDDDDB@BDDDDDDDDDDDECDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12091:1910 -CCGATTTGGATCCGCCGAAGCGGATCGATCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJIIJJIIJJIIJGIJHHIEHDDDDDDDDDDDDDDDBBDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:13182:1885 -CCGATTTGGATCAGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJHJJJIJIJJJJIJJJJJJHHFDDDDDDDDDDDBBBDDDDDDDDDDDEDDDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:13475:1836 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJHIJJIJJJJJIJJJJJHHFFDDDDDDDDDDDDDDDDDDDDDDDDDCDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:14275:1813 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA -+ -JJJIJJJJJJJIJJJIJIJGHFFDDDDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDDDDCCDED4+++4+: -@HWI-ST575:165:C1P5BACXX:1:1101:14914:1754 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -HIIGHJJJFJJDIIJIIJJGGFFDDBBDBBDBDDDBDDDDDDDDDDDDDECDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:15826:1814 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACAGA -+ -GHIDGGCEHHGIIIIGHHGHEBD>BBDBBDDBBBBBB9@?CDDD -@HWI-ST575:165:C1P5BACXX:1:1101:16316:1995 -CCGATTTGAATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -FGG:FDHHGIAFCHIGI@G=:BB<>9@B?BBBDDDBBB@CCCDDDBBDDEAC?CDDD -@HWI-ST575:165:C1P5BACXX:1:1101:17677:1880 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJG0@FGGGEEG@HIGEIGAA9@88/8@?;;65A:3;@@C?@CDD -@HWI-ST575:165:C1P5BACXX:1:1101:18640:1881 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IIJGIGJCHIIIJIIJIIIHHFEDDDBDDDD;BDDBBDDDCDDDDDDBCDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:18890:1897 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIIIJJJJJJJIJJIJIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:1102:2244 -CCACGTCTAGCAGGAAGATCGGAAGAGCGTCGT -+ -IHIIHHGIIH@FGDFIIGJHJJGIIGJHGEFD> -@HWI-ST575:165:C1P5BACXX:1:1101:2310:2103 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT -+ -BADHGGCCCHHGIHIIH;;44=BAB8;/886855@77<<+>CBDDDDB?:3> -@HWI-ST575:165:C1P5BACXX:1:1101:3289:2146 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTCCCGCGTTCCACTCTCAC -+ -IGGFGGGIEGIIJGHGIGJB:BBB>>88<==?@BDBDBD(>CBDDB@?FCH8AAEB<63;@??B@B8ACDDCC -@HWI-ST575:165:C1P5BACXX:1:1101:6143:2137 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -GGD0??)BBB8BBGHFGGHABFB<@?B;<@B=8-AC:?A(4(4>4(++,+:4+4:? -@HWI-ST575:165:C1P5BACXX:1:1101:14357:2064 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA -+ -JJJHHJIJJJJJJJJJJJIGHFFDDDDDDDDBDDDDBBDDDDDDDDDDDEDDDDDDDDDCCCCD4(++4+: -@HWI-ST575:165:C1P5BACXX:1:1101:14498:2106 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA -+ -JJJIJJJJIJJJJIJJIJJHHFFDDDDDDDDDDDDDDDDDDCDDDDDDDEDDDDDDDDDCDDEE4+++++4 -@HWI-ST575:165:C1P5BACXX:1:1101:14632:2166 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJHIJJJIJJJJIJJJJIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDBDCCDEA4(,+++:4:>AB -@HWI-ST575:165:C1P5BACXX:1:1101:14873:2011 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJGIJJJJJJJJJJJJJJHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDDCCDDE4+,,4+::4@C? -@HWI-ST575:165:C1P5BACXX:1:1101:15054:2152 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJJHGICEGGGIJFFIGIIGHFFDDBDBBBDDDDDDBBBCDDDDDDDDDDDDDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:15855:2031 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCA -+ -IJJIGGIGIIHGIIIGGHGFFFFDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:15913:2171 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCT -+ -III:1D?F@<;?=?'0589;8==279@BB8?CDDDDDDDBDECDDCCDD -@HWI-ST575:165:C1P5BACXX:1:1101:16829:2249 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IIIHEGGHIGIIIIIGIJIFFFFDBBBDBDD357BA875@;?9>@CCDCDC -@HWI-ST575:165:C1P5BACXX:1:1101:2836:2439 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJJJJJIJJJIIJJJHEEEHFFDDDDDDDDDDDDDDBD9ACDBDDDDDEDDDDDDDDDDDEDD:++44+:44(42 -@HWI-ST575:165:C1P5BACXX:1:1101:4292:2260 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJIIIJJJJJIIIJJJHHFDDD@DBBBD;@DDDBBDDCCDDDDDB?>CDDDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:4629:2330 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -GEHGBDBFGGCAFGEEGBGEB>BC??BB?@B6555:57??@CDB@<@B?:>C?ACDC -@HWI-ST575:165:C1P5BACXX:1:1101:4537:2454 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJIHBHGGGIJJJIIJGJIHHFFDDD@=BDB@BDBBDDDCDCDDBDDDDEDDDDDDDBCCCDEE:+,+,+:4+4@? -@HWI-ST575:165:C1P5BACXX:1:1101:5316:2437 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJHIIGIFIJJJGJJJFIEGHGFFCDDDDDDDDDDB7BCCCDDDDDDDCDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:5705:2286 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJJJJJIJJJIJJJHHFFDDBBD?BB@DDDBBBDCCDBDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6181:2354 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJGIJIJJJJJJJJJJGHHHFFDDBDDDDDBDDDDBBDDDDDDDDDDDEDDDDDADDDCCDDC:++,4+4+,4>? -@HWI-ST575:165:C1P5BACXX:1:1101:6640:2254 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -JJJIJIGIIJJJIIIIIDIHHFDDB@@@?B@?98B@5:BD@CDBDDBDCDCDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6520:2397 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJIJJJJJJJJJJJJGHFFDDBBBDDDDDDDDDDDDDDDDDDDBDDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:7019:2438 -CCGCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTC -+ -JJJJJJIJJJIJIJJIJJHFFFDDDDDDDDDDDDDD:BDCADDDD@4444( -@HWI-ST575:165:C1P5BACXX:1:1101:15331:2257 -CGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJJJJJJJJJJJGGIHHFDDDDDDDDBDDDDCCD4+++++4 -@HWI-ST575:165:C1P5BACXX:1:1101:16935:2365 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJIIJJJIJGIJIJJGHFEDB@BBBDD6A:9B<=@BD@BB?BDDD8:@CCCDB<5;8ACCCDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:19565:2447 -CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJFHJJJJJJJJJJIIJJHHFFDDDDDDDDDDDDDDDDDDCDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:20585:2479 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -IJJHIJJJIIIJIIJGHIGFHFFCDDDDDDDDDDDB@@DDDCDDDDDDDEDDDDDDCBCCDEEE:4444+:+,+:? -@HWI-ST575:165:C1P5BACXX:1:1101:20888:2259 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -BHIGGHAHG>B9BFHGFHGAE6?A>?BD??D=@BD905@9+37BBBBB?>CAC>@(: -@HWI-ST575:165:C1P5BACXX:1:1101:1310:2696 -CCGTCTGGATCCGCCGAAGCGGA -+ -IJIIGI=FFHHFGGGGGGGEEED -@HWI-ST575:165:C1P5BACXX:1:1101:4969:2645 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -GGIGIIJEIGHGHGHIJFGHHFFCBBBDDBDBDDDBBBD?CCDDDDDDCDDDDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:6485:2694 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJHIJJJIJIJJJJJJJJHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDCDDDDDDCCADDD4+,44(4+44:8 -@HWI-ST575:165:C1P5BACXX:1:1101:6896:2699 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJIDHJGGJGHDFHIIG8AEEFE<@BDDDDDB-75<:>55? -@HWI-ST575:165:C1P5BACXX:1:1101:7123:2615 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJJJJJJIIGJJJJIGHFFDDDDDDDDDDBBD@BDDDDDDDDDD?DDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:7199:2618 -CCGATTTGGATCCGCCTAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJIDIIJJJIIHJIJJGGIGHFFDDDDDDDDDDBBDCDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:10015:2714 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJGHHIJJJJJJJJJJIJIHHFFDDDDDDDDBDBDDBBDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:10105:2714 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIIIGIJJJJJIGJGIJGGFFDDDDDBDDBDDDBHEHFFDDBBB@DDDDDDBBDDCCDDDDDD@DDDDDDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:13944:2746 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAG -+ -IJJIJJIJIIJJJJIJIIIHHFFDDDDDDDDDDDDB@BDCDDD@DDDDDEDDDDDDDDDCCDE@4(++4+ -@HWI-ST575:165:C1P5BACXX:1:1101:14544:2676 -CCGATTTGGATCCGCAGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTG -+ -JJJJJJJJJIJJJIJJJJJIJJHHFDDDDDDDDDDDDDDDDCDDDDDDDEDDDDDDDDDCCDED:(+, -@HWI-ST575:165:C1P5BACXX:1:1101:16211:2706 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -?CG?GBBGGHCHDHIHAAAEHFDCBDBDDDDDBBB@@BACCDDBB@@CDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:17293:2515 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJEHIJJJJJIIJJJJJJHHFFDDD;@BDD@DDDB@BDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:17341:2572 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -@FG:@D?GFDDBFE??;??@BB/75@>;;8?@@'55875:?CCCDDBDDD?@CCACC@C -@HWI-ST575:165:C1P5BACXX:1:1101:18322:2589 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIIJIGJIGEGIIJIIIGGDFDDDDDDDD=:BDBBDDDDCDDDDDDCDDDCDCDC -@HWI-ST575:165:C1P5BACXX:1:1101:18563:2613 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJJJIJJIJJJIJJHHFFDDDDDDDD8@BD@BDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:19030:2538 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -DFD)888BF?8??FGBF@G84@<<@>?;,888:@@::98>C@@DDD<ACA -@HWI-ST575:165:C1P5BACXX:1:1101:19375:2502 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JIIGGFIJIGHJIIIIJJGEHFFDDDDDDDDDDDDDBBBDCDDDDDDDDDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:19630:2588 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -;FH?F@GHIHBD3CHAFBHGHEFCDBDD?BBDDDDB@@?CDDDDBDDBDEDDDDDDDDDDDEED:4444+:+++48 -@HWI-ST575:165:C1P5BACXX:1:1101:1885:2921 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JIJBHIEHGIIIJGJJIHEAHFDCD@>@?BB@BB@BBBD?CCDDDDBB?DCDDCCC@ -@HWI-ST575:165:C1P5BACXX:1:1101:3712:2816 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJIIJJJIJIJJJJJJJJIHHFFDDDDDDDD@BBD@B@DDDDDBDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:3931:2939 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -DFIEH@:CDD;7;@93>C?CDDD -@HWI-ST575:165:C1P5BACXX:1:1101:4575:2908 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -EGIHGEEGHHGGEHGGAAGHEEE?BBB8@@BDDDDEDDDDC@A -@HWI-ST575:165:C1P5BACXX:1:1101:4670:2972 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJIIJIIJJJJJJJJIJIHGFFDDDDDDDDDDDDDDDDDCCBDDDDDDEDDDDDDDBCCDEEE:4++,,:4+4>8 -@HWI-ST575:165:C1P5BACXX:1:1101:4779:2823 -GATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGTC -+ -JIIIIIJJJJIIJJJIEGIHHFDDDDDDDBDDDDB:@CDDDDDDBDDEDDDDDDDDDDDEEE:++44+4++4>? -@HWI-ST575:165:C1P5BACXX:1:1101:7180:2991 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJIFIJIJJJJIJJJJJGIHHFFDDDDDDDDDDDDBBBDDDDDCDDDDDEDDDDDDDDDCADEE:+4:4+4++4>? -@HWI-ST575:165:C1P5BACXX:1:1101:7419:2920 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJGIIJJIIIJIIJJGIIFHFFDDDDDBDDBDBDBBBDDDDDBDDDDDEDCDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:7772:2979 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -HIHIJJIJIIJJIJJJJJHHHFDDDDDDDDDDDDDD:BDDCDDDD@CC5@;;7B<@@C?C@C> -@HWI-ST575:165:C1P5BACXX:1:1101:12044:2834 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIJJJJJJJJJJIJJJJJFHFFDDBDDBDDDDDDDDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12169:2963 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -GJJGIIJI*BFIJJJJHIIHHFFDDDDDBDDDDDDBBBDCDDDDDDDBDEDDDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:13168:2760 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIFHIJJJJJJJJJJIIIGHFFDDDDDDDDDDDDBDBDCCDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:13484:2768 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IIGHGGIJJGIIIJJJJGJGGFFCBBD?BDDBDDDDDDDCCDDDDD@?CDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:13399:2774 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJGIJJJJJJIJJJJIGIHHHFFDDDDDDDDDDDDD:BCCCDBDDDBCDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:13467:2781 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IHIDHGGIJGIIJJJJIJJIHHFFDDDBDDDBDDDBDDDCDDDDDDD?CECCDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:13716:2837 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCCGTTCCACTCTCAC -+ -JJJIJJJJIIIJIIIIIJJGHFFDDDBD?BDDDDDDDDDDDDDBBBDDDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:14400:2843 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA -+ -JJIJJJFIIJIIJFIJIGIHHFFDDDDDBDDDD8BB:CDD:+++4+:,+:>@ -@HWI-ST575:165:C1P5BACXX:1:1101:20783:2769 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGAGTTCCACTCTCAC -+ -IJJGIJJIJIJJIGIIIGIHHFFDDDDDDDDDDDDDDBBCCDB@DDBC:ADDD@CDD -@HWI-ST575:165:C1P5BACXX:1:1101:1673:3017 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -?FGDGH0BDFD?B2BBFA -@HWI-ST575:165:C1P5BACXX:1:1101:2153:3048 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAAACTTCG -+ -;D<0@B=DE@'9?(8;'55<5<@@A@:7&5;B?CCDC?A>::2>::>AC:+4+,(4+++:< -@HWI-ST575:165:C1P5BACXX:1:1101:4715:3184 -CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJJIJJGGGHI>GGHGGGGEEFFCDDDBBDB/59B9BBDADC@BDDDDDDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:4796:3234 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -DHIJJGEHIIIGGCGHEGIHHFFDDB?BBDD@BB@:7@BCDDDBDBBDA@CCCCC:A -@HWI-ST575:165:C1P5BACXX:1:1101:5104:3008 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJIJGIJJJJJIJJGIJJJJHHFFDDDDD@DDDDDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:5272:3008 -CCGGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJDEDGHIJIJIFIIJHIEEFD?BDDDBDDDDDDD@@BBDDDDDDDBDD@CDDDCDC -@HWI-ST575:165:C1P5BACXX:1:1101:5594:3119 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT -+ -CBFEH=BFAGCB)8;@;;AHFDF>>??=58=2:8557@?3@>@BDBDDD3AC -@HWI-ST575:165:C1P5BACXX:1:1101:6927:3052 -CCGGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -DGGEIIGIGGBGF;CGIGGE6BDCCB>BB<@D0CCC -@HWI-ST575:165:C1P5BACXX:1:1101:7098:3130 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJHIJJJJJJJJIJJIIIHHFFDDDDDDDDDDDDDBBDDDDDDDDDDCEDDDDDCDDDCCDEC4(,44+4++4>? -@HWI-ST575:165:C1P5BACXX:1:1101:7842:3018 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JIIIGIGIJGIJIIJJIIIHHFFDDDDBDDDDD@B@@BDDCCBDDDDDCDDDDDDCDDC>>CD>4+,+4+4444:8 -@HWI-ST575:165:C1P5BACXX:1:1101:8316:3056 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIJGI?FHEIJJIGJIIIGHFFDDDDDDDDDBBDDDDDDCCDBDDBDCDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:8582:3236 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -EIBB@BBGIIIEHIEBAEAEHFB9;>>8?BDDD8BBD8@CDDDDDDDDDEDDDDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:10343:3076 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJJJJJJJJIJJJJJHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:10433:3184 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGG -+ -JIJIJJIJJJGJIIIJJJJHHFFDDDDDDDD=BDDB -@HWI-ST575:165:C1P5BACXX:1:1101:10787:3078 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIDFHGGEFGGHIJJIIGHHFFDBB>B?@B=@BB<@BBCCDDBDBDBDDCCDCDDC -@HWI-ST575:165:C1P5BACXX:1:1101:10951:3116 -CCGATTTGGATCCGCCGAAACGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJGHIGGIJGGIJICHGGIHHFFFDDDBDDDBBDDDDDDDDCD;BBBDCDDDDDCCD -@HWI-ST575:165:C1P5BACXX:1:1101:11196:3016 -CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJJHGIIJIJJIIGGIAGIGHFFD/;BDBDD@BDDB@BDCCDD@DDD@CDDDCDCCC -@HWI-ST575:165:C1P5BACXX:1:1101:11257:3175 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJGIIJIJJJJIIJJJIJHHFFDDDDDDDDDDDDBDDDDDDDBDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12061:3148 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJGGGI@FBBGHIJJJJJJGIJGGFFDCA@D:B:BEEFHBEBBABCBBD;BB@<9:B9@CB&5?BCCDCCCCACC -@HWI-ST575:165:C1P5BACXX:1:1101:9555:3271 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JIIGEEDDCGGIIBFHEHHHHFDCBD@B?BD?9BB<9@BCCC@BBDBBCCCDADDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9960:3491 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCT -+ -JJJEHIJIJIGGIJJIIJIFHFFDDD@BDDD?BDDDB@BDCA<@BBBBCEDA?> -@HWI-ST575:165:C1P5BACXX:1:1101:11371:3324 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIJJJJIJJJJIIJJIJJHHFFDDDDDDDDDDDDDDDDDDDBDDDDDDECDDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:11999:3288 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JIGHEHJEIHGIIIJJEIIEHFDDDDDBBDDDBBB<@BDCCDDB;BBBDECCDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12147:3289 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJJJJIIIGIJJJIHHFFDDDDDDDDDDDDDBBDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12666:3312 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIHJJIJJJJJJJJJJJIGHFFDDDDDBDDDDDDDDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12701:3456 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJJJIGHJJJJJFJIFHFFDDDDDBBDDDDDDDDDCDDDDDDDDCDDDCDDCD -@HWI-ST575:165:C1P5BACXX:1:1101:13062:3317 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -HGIJIIIJEIEGADGHG@GHFDFA@>;@:4+:< -@HWI-ST575:165:C1P5BACXX:1:1101:1525:3749 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -GIIIIJCHIIIIIIJJJIIEHFFDD@DBBDDDDBDBDDD3@A9CDA4,++,+444+42 -@HWI-ST575:165:C1P5BACXX:1:1101:2802:3618 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJIFIJJGJJIJJJGJJJJJJIHFDFCCDDBDDDBDDD?CDDBDBDDDDDDDDDDDDCA>CCD4(,+++4:44@< -@HWI-ST575:165:C1P5BACXX:1:1101:3064:3520 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJHIJJFIJJJIJJJIJIHGEFDDDDDDDDDDDDDB7BCDDDDDDBDCEDDDDD@C -@HWI-ST575:165:C1P5BACXX:1:1101:3610:3614 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -IJDHJJFGHIJIEGIJGIIGBFBC@BBBBDD=@BB<@CD?CCC -@HWI-ST575:165:C1P5BACXX:1:1101:4225:3617 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IIDGIECBHIDHJIIEHGIFEFFDD@B?BDD?BDBACDED -@HWI-ST575:165:C1P5BACXX:1:1101:4951:3655 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT -+ -HIIDHE7DBCDHG@BFHGBHA -@HWI-ST575:165:C1P5BACXX:1:1101:4878:3725 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJJIJJJJIJJJHIIHHFFDDDDDDDD?BDDDDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:5530:3596 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJIGIIEIJIIJJJJIJJIFHFFABDDDDDD@DDDBDBBCDD@BDDDDCDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6120:3600 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCAC -+ -HIHB>EC@HHBFEBEF@4@6ADB>BBBB8?;?BDD<75??AC575;@8CDD -@HWI-ST575:165:C1P5BACXX:1:1101:6900:3547 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIEGHIJIJJJJJJJJIIGHFFCBBBDDDDDDDDBDDDDDDDDDDDBDDDDCDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:7633:3566 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -DHEGGE7FCFEGEHGIH8BA:BDA@;BBB=@B@1<:<@FDDHBH84=BDBCAB=@BD?=<8@8@ACCDDDDB?CDDDDADDA -@HWI-ST575:165:C1P5BACXX:1:1101:13315:3747 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -BF5)07@03>C><:AC -@HWI-ST575:165:C1P5BACXX:1:1101:14374:3673 -CCGGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA -+ -JJJJGIGJJJJJJIJJIIGGFFDDDDDDDDDDDDDD@BBCDDDDDDDDDEDDDDDDDDD@CCCD4+++++4 -@HWI-ST575:165:C1P5BACXX:1:1101:14502:3661 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA -+ -JIGDHIGJIGIIIIIIIJIEHFEDDDDBBDDDBDDBCCDBDDDDDEDDDDDDCDD>@CDE:+++4+:44(:< -@HWI-ST575:165:C1P5BACXX:1:1101:2081:3878 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTG -+ -JJIFHIFHIJJJJGIJIJJHHFFDDDDB@DDBBDDBDDD93:9B7BDDDEDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:4430:3860 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -HIEHIIDCGGIJIGIIBHIHHFFDDDDDDDDDDDD@<:@CDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6400:3801 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJIJIJJJJJJIIGGHFFDDBDDBDDDDDDBDDDCCDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6719:3898 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIIJIGIGIJJJEGGGJIHGFFDDDDDDDDBDDB@BBDDDDDBDDDDDEDDDDDCD -@HWI-ST575:165:C1P5BACXX:1:1101:8167:3757 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -HHGGBG7GCFHIEEGEGB6>BB?AC -@HWI-ST575:165:C1P5BACXX:1:1101:9714:3894 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IIIBBDGHGGIIIGGGIJIHHFBCBDBBBDD?<@BB99BCCDBBDDDDCCCDDDDCD -@HWI-ST575:165:C1P5BACXX:1:1101:11290:3835 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIIIJJIJIJJIJJJJIJHHFFDD@BDDDDDDDDBDDDCDCDDDDDBCEDDDDDCD -@HWI-ST575:165:C1P5BACXX:1:1101:12038:3902 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IGHGIEGHGGGIIGEHEHIGHFFDDBDDDDDBB8B<5:BCDDDDBDDBCCADDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12700:3779 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJJJJJIIIJJGJJHHFFDDDDDBDDDDDDBAAA@=@?8@BD@BDBACCBDDB;BCDCCCCCDD -@HWI-ST575:165:C1P5BACXX:1:1101:13831:3779 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJJJJJJGGIJIJJHHFFDDDDDDDDDDDDBDBDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:14731:3871 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIIIGIJIJIJIJIIJJHHFFDD@BDDDDDDDDDDDDDDDDDDDDDDECDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:14772:3935 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -GAEGGIFHIDHECEFHIEGHHFFCB?DDBDDDD8BBBBD?CCDDDDDBDEDDDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:15067:3763 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JIJGIIIJIGIJIJIIIFIHHFFDDBBDBDDDDDD@@BDCCDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:15156:3801 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA -+ -JJJIJIJJJJJJIJJGIIIHHFFDDDBDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDCCCDED4(+,++4 -@HWI-ST575:165:C1P5BACXX:1:1101:16559:3892 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT -+ -CEF):6BD>B? -@HWI-ST575:165:C1P5BACXX:1:1101:1580:4241 -CCGATTTGAATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -IJHFGHEHIJJGJIGJJJJHHFFDB?BBDDDB@BBBDDD3:ABBDDDDCDDBDDDDDEDDDCDDD -@HWI-ST575:165:C1P5BACXX:1:1101:4437:4204 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTCCACTCTCAC -+ -IIJHIGGIIJJJJIIJGIJHGFFDDDDDDDDBDDDDDBDDCCDBBB@DBDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:5319:4088 -CCGATTTGGATCCGCCGAAGGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -GIJJIJEHFIJIIEGHIJIHHEFFEDDDDDDBBB595?CC>6@BBBBDACCCCCDD -@HWI-ST575:165:C1P5BACXX:1:1101:5702:4094 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIHIJJJJJJJJIJJIJJHHFFDDDDDDDD;BDDBBBDCDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:5824:4111 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJIIIJIJJIJIJIJIIIJHHFFDDDDD@DDD@DDDACDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6984:4013 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -GHBEEDFHGGGDF=EEHGHE4@=>?9;8??@B8BDBDBBDDCDDDDB?CDCDCCDDC -@HWI-ST575:165:C1P5BACXX:1:1101:7890:4076 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -FGBHGGGEHGIEIIGIGD@8BECDBD?@BD;=@DDB@?ADDDDDD@?CDDDDCDDD -@HWI-ST575:165:C1P5BACXX:1:1101:8052:4159 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT -+ -D??GAC@BDB?<==??CBBD?88;805575:?>>CDDBB;??AAC -@HWI-ST575:165:C1P5BACXX:1:1101:8080:4229 -CCGATTTGGATCCGCCGAAA -+ -HDFHEG=BF@DHEHGIJBHH -@HWI-ST575:165:C1P5BACXX:1:1101:8526:4228 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGCGGTTTACCGCGTTCCACTCTCAC -+ -JIJJJJIJJIIJJIJJJBGGGHHEDDDDDDDDDDDDDDDDDDDDDDDDCDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:8765:4049 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJJGIJJJJIJJIIJIIJGHHFFDB@BBBDD;@BDBDDDCCDDDDDDBCDDDCDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9822:4042 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJJJJJJJJIJJJJJGHFFDDDDDDDDDDDDDDBDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:10184:4066 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIIJJJJJJJJJJJJJJHHFFDBBDDDDDBDDDBDDDDDDDDDDDDDDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:11794:4113 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJGIJJJJJJJJIJJJJIJHHFFDDDDDDDDDDDDDDDCCCBBDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12356:4148 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JIIHIIIJICGIIJIGIIIEBFFDB>BB@DD@BBD@BDBCDDDDDDDBDECDCDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:14554:4015 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCA -+ -IJJIGIDHIJIIJIHEIJJHDFFDDDDDBDDBDDDBB<@CCCDDDDDDCD -@HWI-ST575:165:C1P5BACXX:1:1101:14736:4035 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -IJICCDDDDDDDEDDDDDDDBCDCDDD4++44+:+4+>? -@HWI-ST575:165:C1P5BACXX:1:1101:3262:4389 -CCGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -FEB@FEFDGIICEFGBF@BB@3070)529>@CCCCDC -@HWI-ST575:165:C1P5BACXX:1:1101:3996:4500 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGT -+ -JJJIIIJJIJJIJIJJGIGEHHFFCDDDDDDDBDDD:BDCDDDDDD?CDDDDDDDDDCDCEEE:+,,,+4444:29 -@HWI-ST575:165:C1P5BACXX:1:1101:4255:4286 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAA -+ -?D<00)0????B3BDBF;E@8888,3'005BDBDDDD?BDDB<:BDDDDBDDDDDEDCDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:5886:4450 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -IJBHGGIIGGGGIGEHGGGBHDB?>;;;@CDDD7;2/28?8'07@55<4:<@:A -@HWI-ST575:165:C1P5BACXX:1:1101:9397:4390 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JIIIIIGIJIIIJIJJGIJHHFFDDBDDDDDBBBBBDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9626:4444 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIIJJJJJJJJJIJJJJJHHFFDDDDDDDDDDDDDDDDDCDDDDDDDDEDDDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:9920:4375 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJIJJJJJIJJJJJIJJHHFFDDDDDDDD@DDDBDDDDCDDDDDDDDEDDDDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:9928:4484 -CCGATTTGGATCCGCCGAAGCGGATCGTTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -GI:@:BFHIEGHIIIGIFIBEDB<@BDCBDDBCBBBBBBCCDDDDDD@CDDDDDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:10347:4290 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJJJJJJJJJJJIJHHFFDDDDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:10607:4474 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTATCAC -+ -JIIGGIJJJJJJJIJJIJIHHFFDDBDBDDDBDDDDDBDDDCDDDDBDDEDDCCDDD -@HWI-ST575:165:C1P5BACXX:1:1101:11109:4422 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGCGGGGTTTACCGCGTTCCACTCTCAC -+ -CB@6?88D>*9??FEDG@E=;<@;&)07@B5?9A:5C<:ACD -@HWI-ST575:165:C1P5BACXX:1:1101:11719:4366 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJJJJJJJJJJIIIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:11803:4357 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIGIIIIJIJJJDIJHIJHHFFDDDDDDDDBDDBDDDDCCDD@DDBBDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12622:4263 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -FGIIJJIIBGIHGIJIEAE6@BBDBBD5550??A@BBBDBBC3:>9CCDD -@HWI-ST575:165:C1P5BACXX:1:1101:13888:4376 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJJGIIIHI>GGEHGIHBGEBEFDDBDB@DD@B8B@'=BB'9?8;BBBBDB)5ABD59=;;@BB@5<9:(5<@;B8CC -@HWI-ST575:165:C1P5BACXX:1:1101:21156:4355 -CCGATTGGGATCGGCCGAAGCGGATCGGGCGAGGGGTTTACGGCGTTCCACTC -+ -?1?8@F(?8?);-4'---599><3''38'55077<@@B@?C(57755?(+4>9 -@HWI-ST575:165:C1P5BACXX:1:1101:21005:4492 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -GFFGIICIIIHIEHGIDHEEEFDA@DDDDD=@DDD57?CC:97BDB?CDCDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:2208:4721 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJGHIJGIJJJJJJJIIJHHFFDDBDDDDDBDDBBDDD>ACB@BDDDDEDDDDDDDDDCCDDD:++(,+:34+>8 -@HWI-ST575:165:C1P5BACXX:1:1101:3594:4541 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IGHHIJIJJJJFBHHIEIEHHFDDDDDDDD=@DDDD9BDDDDDDBBBDEDDDCDDC -@HWI-ST575:165:C1P5BACXX:1:1101:4453:4680 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJIIIIJIIJJJJIJJIJDHHFDDDDDDDDDBDDBBBBDDDCDDDDDDCDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:4574:4514 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC -+ -JJJIIJIJJJJIJGHJIJIHHFFDDDDDDDD;BBDBBDDDDDDDDDDDDEDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:4512:4554 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -IIJIJJFIJJJJJIJIIIGHHFFDDDDDDDD?DDDDDBDCDCBBDDDDDECDDDDDDDDDCDDE:44+,+:4+4>? -@HWI-ST575:165:C1P5BACXX:1:1101:6091:4740 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -CFF0:BDGG38?BBCBF8328;=;@B@9@<9?*).3,8,823)-77B5??>ABDDD;(9 -@HWI-ST575:165:C1P5BACXX:1:1101:14064:4581 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAG -+ -IJJIJJIJJIJJJGIJIGGFHFFDDBDDDDDDDDDBDBBCDDDDDDDDCDDDDDDDDDCCCEED:+++4+ -@HWI-ST575:165:C1P5BACXX:1:1101:14735:4724 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIGJIJJIJFIGIIJJGEHFFDDDDD1;B;@BDB@CBDDDDDDEDCDCCDD -@HWI-ST575:165:C1P5BACXX:1:1101:18593:4710 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJJIIHEHCHIJJIJIGGGEEFFDDBDB?BB/7A8@???FFEDHEG<>C9>:@C -@HWI-ST575:165:C1P5BACXX:1:1101:16043:4995 -CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCCTCTCAC -+ -IJJHIJJJJJJJJIJIIJJGGFFDDDDDDDDDDDDBEFF>BDB?BB?8;@B578>>CDDB<;8<3:>GIIIJJJHHFFDDDDDD?BDDDDDDDDDDBBDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:18453:4916 -CCGATTTGGATCCGCAGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -EGG:FEGHHHGI;FH? -@HWI-ST575:165:C1P5BACXX:1:1101:19870:4937 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJHGIJJJIIGHIEHIIIGHFFCDBDD@BDBBDDD7@BCDCBDDDDD8 -@HWI-ST575:165:C1P5BACXX:1:1101:19796:4989 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJJJJJJJJIJJIJJJJIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDCCDEEE:+,+4+:4+:>8 -@HWI-ST575:165:C1P5BACXX:1:1101:20053:4972 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC -+ -IIDDHGIJHGGIGHIIDIHHFF>ABDDDDD?DDDDBBFGGIH?B@ABGE3=>?@@BBBDBD8?33>:&57@8ADDC<@@CC?AADD(::4>:44:+:4:? -@HWI-ST575:165:C1P5BACXX:1:1101:1686:5115 -CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJJJJJHIGHHIJJJGJIHHFF@B@BBDDDBDDDBBDD9@CBDDDDDCDCCDDDACB@ACEDD:+444,:>>>4< -@HWI-ST575:165:C1P5BACXX:1:1101:2938:5127 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -HIEDFHIG< -@HWI-ST575:165:C1P5BACXX:1:1101:8840:5173 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJGIJJJJGIJJJJJJIJFBEFDDBDDDDD=BDDBBDDDDDDDDDCDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9731:5160 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJDFHJGIIIIJJIJJIJJGHFFDDDDDBDD;BDDDDDDCDCDDDDDDDEDDDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:10830:5146 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IGHIIJIIJJIFEEIJBHABHFDDD??DDDBD@@BD:BDCCDDDBB?CDCCCDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:11032:5068 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJIJJIIJJIJJIJJJGGFFDDDDDDDD;BBDBDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:11565:5085 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIEHHIJJHIJJJJJJIIFFFFDDBDDBDD?BBD<<@BDDDDDDDD?CEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12209:5017 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC -+ -?D)00B0BDF>*?CGB@5AA<9638'9=8,2257855:@C:>@50552<3>:9 -@HWI-ST575:165:C1P5BACXX:1:1101:12070:5238 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIHIJJJJJJJJJJJJJIHGFFDDDDD?BDDDDDDDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12614:5234 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIHIJJJJJJIJIJJJJJHGFFDDBDDDDDDDDDDDBBDDCDBDDDDDEDDDDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:12934:5066 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIJIJJJJJJJJJJJJJGEFFFDDDDDBDDDDDDBDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12889:5153 -CCGTCTTGGATCCGCCGGAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -JJGHIJJGIIJJJGHIGIHFFFDDDDDDBBD1BDD@57BCDCD@DDDBDECDDCDC -@HWI-ST575:165:C1P5BACXX:1:1101:13006:5008 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT -+ -:C????;F98??B=1;AEG4-=5=>/,'(,,,))57@5??CCDDDD@2?C@C -@HWI-ST575:165:C1P5BACXX:1:1101:13701:5054 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIJJJJJJIIJJJJJJJGHHFFDDDDDDDDDDDDDDDDDCDDBDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:13818:5190 -CGATTTGGATCCGCCAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJHIJJJJJJJJGIJJJHIJJJJJHHHFDDDDDD=8?CDCDDDBDDCCDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:14721:5211 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC -+ -DHBHIF@GHIGEGGIBHG6EHEDFDDDDB@;?BDDBCCC:@@CD -@HWI-ST575:165:C1P5BACXX:1:1101:15180:5074 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -)CGHIJIJJIJJJIIIGGHHHFFDDDDDDDDDDDDDDDDCDDDDDDDDDEDDDDDDDDDDCEEE4+++++::4>C@ -@HWI-ST575:165:C1P5BACXX:1:1101:15733:5028 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJJHIJJJJJJJJIJJJJJHGFFDDDDDDDDDDDDDBDDDDDDBDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:16783:5148 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IIIHJJIJJJIJIIJJIJIGHFFDDDDDDDD?BDDB@BBDDDBB@DBDDEDDDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:17093:5065 -CCTATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJJJJJJJJJJJJIGIHHFFDDDDDD@DDDDBBDDDDDBDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:18177:5052 -CCGCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJJJIIGIFGGJIGHA/@BEBD?>BDDDDDDDDDD05?CDDDBD;7GIGHBBBHEBABBBD@BDDDDDBBBBDDDDDDDDDDEDDDDDCC -@HWI-ST575:165:C1P5BACXX:1:1101:21070:5172 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -?F<086BFHE8?B,8=BDDDB758>CABD??@8:4+4444>:4+ -@HWI-ST575:165:C1P5BACXX:1:1101:2332:5476 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIGIJJJJIJJIJJJJEHHHFFDDDDBDDDDDDDBBBDACCDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:3549:5422 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJJIJJJIIJJJJIJJGIJHHFFDDBDDDDDDDDDBDDDCDCDDBDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:3934:5255 -CCGATCTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -IJJGG6DHIJJJIIJJIJFHFFDDDDDDDDD@BDDDDBDDDDDDDDDDDEDDDDDDDDDCCEED:44+,+:++4>? -@HWI-ST575:165:C1P5BACXX:1:1101:3779:5257 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -IJIIJJJIJJIIJJJJIJIHHFFDDDDDDDDBDDD@BBDCCCDBDDDDDDDDDDDDDDDDDDDE::44,+:444@? -@HWI-ST575:165:C1P5BACXX:1:1101:3981:5396 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -CGD@FAFIIIEGEGEBFGGBGIDFEDDC=>?>BBD<95?ACCDDDDBBDECDCDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:4352:5435 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJJIJJJJJJJJIIJHGFFDDDDDDDDDDDDDDBDDDDDDD;@DDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:5757:5381 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -CFG>BGBFGGHD@BFFHGGEEED?8;?528@95B7<529ACBDDDD?CD:A<@@CC -@HWI-ST575:165:C1P5BACXX:1:1101:1536:5742 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -HJIEIJIGGBHGICIIBHIGHFFDBBDD?BDB9BDB5CCDDDDDDDEDDDDDDDDC@CDEE:++4,+44+(:2 -@HWI-ST575:165:C1P5BACXX:1:1101:4347:5721 -CAGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IIHGIGAAEHIIIEDHEHJIJHFFFDDDDDD:@DBB77BCCCBDDDDDCDCDCCDDC -@HWI-ST575:165:C1P5BACXX:1:1101:5268:5661 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -CF:)8)8DDB;;88;8'00:7@889AA5@BDD@DECC?CDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6568:5658 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIGIJJJJJJJIGJJGGGHGFFDDDDDDDDDDBDBBBDDDCDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:7344:5519 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JIGHIJJJIJJJJIIJGGHFHFFDDDDDDDDDDDDBBBDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:8216:5553 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJEIJJJJJIJJIJJJGGEHFFDDDDDDDDDDDDDDDDDDDDBDDDDCEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:8183:5554 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC -+ -?DFHI;?BC<:ACD -@HWI-ST575:165:C1P5BACXX:1:1101:12148:5538 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJEIJJIJJJJJJJJJIIFGFFDDDDDDDDDBBDDD@BDBBBDCDCDDCCDC -@HWI-ST575:165:C1P5BACXX:1:1101:13615:5616 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJHIJJJIGJIJJIJJJIHHFFDDBBBDDDDDDDDDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:13537:5735 -CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJIJIIIJJJIGIIGGHFDDBDDDDDDDDDBBBDCDDDDDDDDDEDDDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:14584:5542 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA -+ -JJJIJJJJJJJJJJJJJJJHHFFDDDDBDDBDDDDDDDDDDDDDDDDDCDDDDDDDDDDCCDEE4(,+++4 -@HWI-ST575:165:C1P5BACXX:1:1101:15724:5746 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC -+ -IJJIJJJIJJJJJJJJJJJHHFFDDDDDDDDDDDDDB@DDCDDDDDDDDEDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:17227:5625 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTC -+ -FGIB?B*?3?BBFBFG6A8;:EB<9A?=;;;289@&0893@BD;5;(+:>C9>@ -@HWI-ST575:165:C1P5BACXX:1:1101:17881:5507 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJJIJJJJJJJJJJJHHFFDDDDDDDD8@DDBDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:20112:5554 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ ->GI:D;DHFG@B8BF;AEHEEDD>@>>B5=BDDDD@B@<>CCDDDDDBDECCCDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:20322:5548 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -BHI06??DGE/;3>B?@B=BB8700CC<(+: -@HWI-ST575:165:C1P5BACXX:1:1101:21233:5679 -CCGATTTGGAGCCGCCGAAGCGGATCGGGCGAGGGGTTTACCGCGTTCCA -+ -1?D)0)0?99)9(7@'5':<<>6'9'88'27BB8B970(+>37BDBD?CD -@HWI-ST575:165:C1P5BACXX:1:1101:1959:5920 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJHGJIJIJJJJFIJJGIFHFFDDDDDDDDBDBDDDDD?CCDDDD;@CDDDDCDDDDDCCDED:44(4+4,4+>8 -@HWI-ST575:165:C1P5BACXX:1:1101:1924:5978 -CCGATTTGGATCCGCCGAGGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -IJJIIIGJJIGHIJIGJGHGFDDDDBDDBD85:BDBDDBACDDDDDDDCDCDDDDDDDDCCEDE4+,44+4+++:8 -@HWI-ST575:165:C1P5BACXX:1:1101:4153:5778 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -GIJGIGGIJJJJIJJIGIIHHFFDDDDDDDDDDDD@<@C -@HWI-ST575:165:C1P5BACXX:1:1101:6188:5778 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIHJJJJJJJJJJJJJJJHHFFDDDDDDDDDDDD@BBDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:6867:5946 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGTTCCACTCTCACCATTTCTAGTGAGACTTCGTC -+ -JJJIJJJJJIJJIIJJIGIHHFFDDDDDDDDDDDDDDBDDDCDDDDDEDDDDDDDDDDDEEE@44>44:+,4>?CB -@HWI-ST575:165:C1P5BACXX:1:1101:7535:5814 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IIGIJIJJJJJJIJJJGGHGFFDDDDDDDDDDDDDB7@CDDDBDDBBCDCDDDCAC -@HWI-ST575:165:C1P5BACXX:1:1101:7794:5903 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIGIIGGIJJJJIIJHIJHHFFDD@DDDDBDDDDDDDDDCDDDDDDBDDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9106:5832 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIIIIJJJJJJJJIJIIEEFFDB@@BBDD;:BEDC@DDB?BB-95<05<9:CDD75<ACCCCC -@HWI-ST575:165:C1P5BACXX:1:1101:9549:5793 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -DG>@FG6BFAGGICGHGGIBEEDCD?>8@??;@@BBBBBCDDDDDDBBCDCDDCDDD -@HWI-ST575:165:C1P5BACXX:1:1101:10541:5867 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJFHJJJJIIJJJJJJJJHHFFDDDDDDDDDDDDBBBDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:11397:5818 -CGATTTGGATCCGCCGAAGCG -+ -H?)@DGI@DD4+++++4 -@HWI-ST575:165:C1P5BACXX:1:1101:14716:5991 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JIJGIJJJJJJJIEHJIJIHHFFDDDDDDDDDDDDDB@DDDCDDDDDDDDDDDDDDDDDDDDDE:,++4+:44>A@ -@HWI-ST575:165:C1P5BACXX:1:1101:15153:5829 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGAGTTCCACTCTCACCATTTCTAGTGAGA -+ -DHIHIGGIJIJJGIJIJGGFHFFDDDBDDDDBDBDBDCADD4+++++4 -@HWI-ST575:165:C1P5BACXX:1:1101:15618:5813 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -HGGGGHIJIEGBFFEGHDGHHFFDBBBB@==89@@<07@>@CBDDDBD?DCDDDDA -@HWI-ST575:165:C1P5BACXX:1:1101:16278:5962 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAAGGGTTTACCGCGTTCCACTCTCA -+ -GHIABFHDGGIG>FGIGFHFDFDBD?BDDDD8BBD@BABC:AB;BDDDCDDDDCDA -@HWI-ST575:165:C1P5BACXX:1:1101:17404:5768 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JIFDGHIJJJJJJJJJIJIEHFFDD@BDDDD;@DDBBBDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:18508:5770 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJIGIJJJJJJJJIJJIJIHHFFDDDDDDDDBDDDDDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:18654:5893 -CCGATGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGTC -+ -IJJJJJJIJJIIJJJFHCHIIGHIEHFFDDDDB@DBDDDDDDDBDDDDDDDDDCDDDDCCDDDCC@ADD:(++,+4++4:2 -@HWI-ST575:165:C1P5BACXX:1:1101:20461:5768 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -IJJDFFHGIHIIJIGGIJJHFEDCD>BD@BBB@DBBBBDDCDDDDD@?AA@CCCA> -@HWI-ST575:165:C1P5BACXX:1:1101:1693:6048 -CCGATTTGGATCCGCCGAAGCGGGTCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -IGGGHGGGHEGHGHGIIDEGAHFBCDDD@@D9:B8::B?3:ABB<@DD?:CC<@@CCDCCCACC444:4+:,44>8 -@HWI-ST575:165:C1P5BACXX:1:1101:3291:6004 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJIIJIIJJJJJIJJGHIHHFFDDDDDDDDDDDDDDBD?CDDDDDDDDEDDDDDDDDDCCEEE:4444(44++>8 -@HWI-ST575:165:C1P5BACXX:1:1101:3937:6198 -CCGATTTGGATCCGCCGGAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIHIJJJIIIJJJJJIJHHFDDDDDD'8@DBBDDDDDDDDDDDDDDDDCDDDDDCC -@HWI-ST575:165:C1P5BACXX:1:1101:4273:6038 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIGIGIIIIIIJIGIIIGFHFFCDDDDDDB:@BDBBDDC>ABBDDBDCDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:4445:6115 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -HGFC>CA -@HWI-ST575:165:C1P5BACXX:1:1101:4344:6153 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJIJJJJJJJJIJJJGBHFGFFDDDDDDDD@DDDDDDDACDDDDDDDDEDDDDDDDDCCCDEE4+++,+4444@? -@HWI-ST575:165:C1P5BACXX:1:1101:5212:6077 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JIJIIIDCGGHIJBHHIIGEH@BBDDDD;B@DDBDBCCCDBDDBDCDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:5854:6193 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACT -+ -JJIIJJJJJEHIJJJJJJJHHFFDB@BBDDD:@BDBDBBCDDDDDD;BDDCDDDDDDDDCCDEE>4444+4+4 -@HWI-ST575:165:C1P5BACXX:1:1101:6093:6246 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -JJIEIJGHIIIJFJJGGBGEHFB>B>B@BDD25BCDHIFEHHIIEA6AAEE6<>@B@??@/7CDD -@HWI-ST575:165:C1P5BACXX:1:1101:8253:6223 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGGTTACCGCGTTCCACTCTCAC -+ -DGFH=DD?3BFDCCDA;'@8@CD -@HWI-ST575:165:C1P5BACXX:1:1101:8552:6031 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -FE0@FGCGBGEHIIGJGABEHFCDDBDDDDDBDBD578ACABFFFEAGCDDB5<<<4@CACCCD -@HWI-ST575:165:C1P5BACXX:1:1101:9569:6015 -CGGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJJIJJJJJFIJJJJJJJHGHFDDDDDDD/=5:BBBBBDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:10905:6024 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -CCD@?@8D@*9?DHDBG@?CC -@HWI-ST575:165:C1P5BACXX:1:1101:11477:6010 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT -+ -JIIGIJIJJIIIJJJIIIIEHFFDDBB@?BDBBDDDDBBDCDDBBBD?ADDD -@HWI-ST575:165:C1P5BACXX:1:1101:12389:6154 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -JJJIJJJJJJJJJJJJIGIEHFFDDDDDDDDBDDDBDDBDDDDDDDCDDEDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12820:6019 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -GGHE>HGHGEGGIJE;FHI>6BDDD@BDDDDBB@DDB8?CCD57@@B@DD:A9A:@C -@HWI-ST575:165:C1P5BACXX:1:1101:13503:6047 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJHIJJIJJJIIIIJJGIGHGIHFFDDDDDDDDDDD:BDDCDBDDBBCEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:14071:6041 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JGB@FHIHIEI@BFEDHGIHHFFDDDD=B?B@BBBGIGGFFDBDDDDDDBDDDB:BCCCDBDDB?ACDDCDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:17938:6081 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJGJJJJJFIJIIJJIJJHHFFDDDDDDDD=BDDDDBDDDDDDDDDBDDCDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:19315:6150 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -B8CF)?69B@B*9?BFGB8@AADFABDB?;@=?B<5)5?3:AB@75;BCDCDACCDC -@HWI-ST575:165:C1P5BACXX:1:1101:19532:6018 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIIIJJJJJIGIGHIIJEFGFFDDDDDDDDDDDDB5CDBB@@D7CDCDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:1089:6262 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIBFHGIJIGHHIHIJGHBCA4(::4(4:8 -@HWI-ST575:165:C1P5BACXX:1:1101:1829:6451 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGATTTACCGCGTTCCACTCTCAC -+ -JJJIJJIJJEIIJEHIEGIHHFFCDBDDBDDDBDDDACCDDDDDDDDDADDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:2484:6444 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -G?@DFC=FG*9?D9BFHGHABBB83<@:=B-9@BACBDDDB8?DCCA@ACC<@>@@DC:44+,++(,:>? -@HWI-ST575:165:C1P5BACXX:1:1101:3874:6280 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJJIJJJIJJJIJIIIIJIEHFFDDDDDDDDBDDDDDDDACDDDDDDDDDDDDDDCDBCCDDDE:+44,+:44:C@ -@HWI-ST575:165:C1P5BACXX:1:1101:4404:6415 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJIHIJJIIIJJJJJJIIIFHFFDDBDDDDDBDDDDDDDDDDDDDDDDDEDDDDDDDDDDDEED:++4,(44+4:< -@HWI-ST575:165:C1P5BACXX:1:1101:5416:6451 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -H@GD@?;FDBFHHIBHGG>EB?DCDDDDDDDDDDB<57@>:>@<@@@BCCDCCDCCDBC:::CC4+++,+:4+4>8 -@HWI-ST575:165:C1P5BACXX:1:1101:7066:6455 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJFJJIJJJJIJIJIHHFFDDDDDDDD?BDDDDDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:7488:6251 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJGJJJJJJJJJIJJIIIHHFFDDBDDDDDDDDDBBDDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:7486:6329 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIJJIJJIJJJIJJJGHHFFFDDDDDDBDBDDDDB7?CCDDDDBB??ACCDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:7643:6450 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGT -+ -JEDFHJIHICIGIGIJGJJHHFDAB>?=??==@BBBB@DBB -@HWI-ST575:165:C1P5BACXX:1:1101:8454:6487 -CCGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IIGHJIJICHGHHIJJGBGFFFDBBDDDDB?BDBD55?C>CBDD@B8<>ACCDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9137:6329 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIFIJIJJIJJJIJJJIIHFFFDBBDBDDDBDDDDDBDDCDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9587:6277 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTATCGCGTTCCACTCTCAC -+ -IIBGEIIJJIJJJIJJJGHEHFFDD@DDDDDBDDD<7ADDD4(++,+444:@( -@HWI-ST575:165:C1P5BACXX:1:1101:10054:6289 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGTTCCACTCTCAC -+ -JJJGIJJIJJIJIIJJGHGHHFFDDBDBBBDDDDDDB@BCCCDBBCCCCCDDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:10173:6294 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGGGCCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGTC -+ -JJIGEIJIJJJJJJJJIJJHHFFDDDDDDDDBDDDDDDDDDDDDDDDDCACDDDDDDDDEEE@::4,+4++4:?C@ -@HWI-ST575:165:C1P5BACXX:1:1101:10764:6333 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT -+ -HIJIJIGICGGIIEHIABEGHHDEC?>>;?B8=85757@ACDDBDDBDCCC@ -@HWI-ST575:165:C1P5BACXX:1:1101:11047:6477 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT -+ -GDGDGEGGFFBB?CDEB;EEEEF<>;?@5;?8<58<:@B>C>@;BBDBACCC -@HWI-ST575:165:C1P5BACXX:1:1101:11411:6372 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC -+ -JIJIJIDIHIJJJFGHBGGHHFFDDBB=?BB?BDB@8@BCDDDDDDDDCDDCD -@HWI-ST575:165:C1P5BACXX:1:1101:11665:6448 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJIIJJJIIJJIJJGIIHHFFCDBDDDDDDDDDBBDDDCDDDDDDB?DDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12774:6457 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -GIBDHIGGIJIIJEIF@@EAEFBBDB@?@@?;8BDBDB?CC>9B7@@BBB@@@BB00??>@507;<<<>CCDDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:18173:6282 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJIHJJIJJIGIJIJIIIJHHFFDDDDDDDDDDDDDBDDDCDDBBDDBCEDDDDDDC -@HWI-ST575:165:C1P5BACXX:1:1101:18209:6492 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJHIGIJJHJJJJJJJJIFHFFDDBDDBDDBDDDDDDDDCDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:18546:6430 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJJHIJJJJJJJJJJJJIGFFFDDDDDDDDDDDDD<@DDCDDBDDDDBDEDDDDDCD -@HWI-ST575:165:C1P5BACXX:1:1101:19023:6315 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JIIJJIIIGFHJJJIJBHEHGHFFDDD@BDBDDDD57@CCDDDD<@8@CD;BDDDDECDDCCDDBCDADAC::434+:4:::? -@HWI-ST575:165:C1P5BACXX:1:1101:20043:6415 -CCGTCTGTGTCCGAGATCGGAAGATCGTCGT -+ -FHGGG@F0??4?F(<:@FGG@DHCEHH3;B> -@HWI-ST575:165:C1P5BACXX:1:1101:1982:6500 -CCGGTGTCCAGATCGGAAGAGAGTCGT -+ -JFGE8@?FHGGCBCEIIGIDHHIHEEH -@HWI-ST575:165:C1P5BACXX:1:1101:1934:6651 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -JJIGIJJJJIEIJGIJIJIHHFFDDDDDDDDDDDDDDDDDCDD@BDDDDEDDDDDCCBDDDEEE:44:4+:4+4>0 -@HWI-ST575:165:C1P5BACXX:1:1101:3031:6587 -GATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA -+ -JHIIJIJJIJJGIIGHHFGIIHFFFDDDD@DDDD<:?CDDDDDDDDDDDDDDDDDDDDDEEE>++:44: -@HWI-ST575:165:C1P5BACXX:1:1101:3659:6632 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACT -+ -JJJHIJIIJHGIJJJJEHJHHFFA@BBB@BD@DDD@D0078344 -@HWI-ST575:165:C1P5BACXX:1:1101:6970:6733 -CCGATTTGGATCCGCCGAAGCGGATCGGTCAAGGGGTTTACCGCGTTCCACTCTCAC -+ -IIFEGHIIGGGEHGEGHGHGEHDFC>@>@BB>CCDBDBDDDDDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:9786:6627 -CCGATTTGGATCCGCCGAAGCGGATCAGTCGAGGGGTTTACCGCGTTCCACTCTAAC -+ -JJJIJJJJJJIJJJJJJIJFHFFDDDDDDDDBDDDDDBDDDDDDDDDBDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:10466:6616 -CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJEHJJJJJIJIJJJJGGHBEBFCC@DBBDDBDDDD9@DCDDDDDDDBDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:12094:6626 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIIJJJJJJJJJJJIIIHFFFDDDDDDDDDDDDDDDDDDDDDDDBDDEDDDCDDD -@HWI-ST575:165:C1P5BACXX:1:1101:13236:6588 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -IJJHJJJJJJJJJIJJIJJHHFFDDDDDDDDDDDDBDDDDDCDDDDDDDEDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:13123:6721 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCCGTTCCACTCTCAC -+ -JJGFHGIIJJGIJIIJIIJFHFFCDDDDDBDBBDBBBBBDDBBD=BDD@5<@?:@B@;>@>HII@GIIGJFGGGIJH8=DDBB@BBDDBDDDB07?>ACDBBD@BCDDDDDDCD -@HWI-ST575:165:C1P5BACXX:1:1101:20819:6735 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG -+ -IJJIJIJJJEEIGIJGGGHHHFFDDDDDBDDDDDDDDBDACDDBBDDDCDDDCDDDCBCC@DDE:+,+4+:+4:@? -@HWI-ST575:165:C1P5BACXX:1:1101:1564:6811 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -HIBEDH;DCFHHIIJIJGGBGGAED -@HWI-ST575:165:C1P5BACXX:1:1101:1927:6913 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJE@BBDD8<< -@HWI-ST575:165:C1P5BACXX:1:1101:6911:6752 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJJJJJJJJJJJIJJJIJHHFFDDBDDDDDBDDDBDDDDDDDDDDDDDEDDDDCDD -@HWI-ST575:165:C1P5BACXX:1:1101:8402:6992 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -HGE?FD;GBCDDDD?8@87@??BBBDD@75@@ACCDBBBBBA:AACCA -@HWI-ST575:165:C1P5BACXX:1:1101:17036:6768 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JIIIGGGIGEIIJJDIIFIEEFEDCBDDDDDDDDD@BDDCDCDDDDDDCEDD?CCDD -@HWI-ST575:165:C1P5BACXX:1:1101:17542:6830 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJJJJJJIIIJJJIIHHFFDDBDDDDDDDDDDBDDCCCDBDDDBCDDDDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:18093:6840 -CCGATTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA -+ -GIIIGGIJ3DHDH=FE/@EEDFAA8?BDBBDDDB@)5?9:>@5(88;;-750023>307;@B?C@CA -@HWI-ST575:165:C1P5BACXX:1:1101:19230:6988 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJJJJJJIJIJJIHGFGFFDDDDD@BDDDDDDBDDCDDDB@BDDDCCCDDDDD -@HWI-ST575:165:C1P5BACXX:1:1101:20486:6800 -CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAG -+ -JJJJIJJJJJJJJIJJIJIHHFFDDDDBBDDDDDDDBDD?CDDBDDDDDEDDDDDDDDDD@DDD:++44+ -@HWI-ST575:165:C1P5BACXX:1:1101:20297:6938 -CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JID@GIJJHGHGGIGI;ABEFBBDDDDDBDBDDDD@5@DDDDDDDDBDCAC:4>:4:@8 -@HWI-ST575:165:C1P5BACXX:1:1101:1500:7064 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJJIJJJJJIGIJIIJJJJHHFFDDBDDBDDDDDDBDDDDDDDDDDDBDEDDDDCCC -@HWI-ST575:165:C1P5BACXX:1:1101:1577:7179 -CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJDEGHHJJJJI@HIIGEGH:B?88;=;9BD@:<@CDCD;A9@(4@ -@HWI-ST575:165:C1P5BACXX:1:1101:2297:7148 -CCGTCTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC -+ -JJIGIJIJIHIJIJIGGIBEADBCBDDDDDDBDDD97@CCD@DB;;?CCCDDDDDD diff -r fb76303acf4f -r 431aebd93843 rna_probing/test-data/trimming_stats.txt --- a/rna_probing/test-data/trimming_stats.txt Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,1 +0,0 @@ -No trimming: 534 , out of which not recognized MD field for: 0 ; 1 nt trimmed: 90 ; 2 nt trimmed: 14 ; 3 nt trimmed: 9 diff -r fb76303acf4f -r 431aebd93843 rna_probing/test-data/unique_barcodes.txt --- a/rna_probing/test-data/unique_barcodes.txt Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,108 +0,0 @@ -RNase_P 110 187 2 -RNase_P 111 187 4 -RNase_P 112 187 2 -RNase_P 115 187 1 -RNase_P 117 187 5 -RNase_P 117 188 1 -RNase_P 119 186 1 -RNase_P 134 188 1 -RNase_P 137 187 1 -RNase_P 138 187 1 -RNase_P 152 187 1 -RNase_P 10 187 1 -RNase_P 11 187 1 -RNase_P 15 187 2 -RNase_P 30 187 2 -RNase_P 31 186 1 -RNase_P 31 188 1 -RNase_P 18 186 1 -RNase_P 18 187 1 -RNase_P 32 187 1 -RNase_P 19 187 1 -RNase_P 33 187 1 -RNase_P 50 187 2 -RNase_P 51 187 1 -RNase_P 38 187 1 -RNase_P 39 187 1 -RNase_P 55 187 3 -RNase_P 56 187 1 -RNase_P 2 187 2 -RNase_P 57 187 2 -RNase_P 3 185 1 -RNase_P 3 186 9 -RNase_P 3 187 94 -RNase_P 3 188 1 -RNase_P 58 187 3 -RNase_P 71 187 2 -RNase_P 59 187 1 -RNase_P 4 185 1 -RNase_P 4 186 32 -RNase_P 4 187 289 -RNase_P 73 187 1 -RNase_P 5 187 14 -RNase_P 6 186 2 -RNase_P 6 187 10 -RNase_P 7 187 4 -RNase_P 8 187 4 -RNase_P 9 187 3 -RNase_P 77 187 4 -RNase_P 90 187 2 -RNase_P 78 187 2 -RNase_P 92 187 2 -RNase_P 93 187 1 -RNase_P 94 187 2 -RNase_P 97 186 1 -RNase_P 97 187 4 -RNase_P 100 187 1 -RNase_P 101 187 2 -RNase_P 98 187 1 -RNase_P 99 187 5 -RNase_P 102 187 1 -RNase_P 103 187 1 -RNase_P 104 187 1 -RNase_P 105 187 1 -RNase_P 106 187 2 -RNase_P 107 186 1 -RNase_P 107 187 3 -RNase_P 121 187 2 -RNase_P 109 187 2 -RNase_P 125 187 1 -RNase_P 126 187 2 -RNase_P 128 187 3 -RNase_P 129 187 1 -RNase_P 142 187 1 -RNase_P 145 187 1 -RNase_P 147 187 1 -RNase_P 148 187 1 -RNase_P 163 187 1 -RNase_P 169 187 1 -RNase_P 20 187 1 -RNase_P 21 186 2 -RNase_P 21 187 7 -RNase_P 22 187 2 -RNase_P 23 185 1 -RNase_P 23 187 4 -RNase_P 24 187 1 -RNase_P 25 187 1 -RNase_P 27 186 1 -RNase_P 27 187 1 -RNase_P 40 186 1 -RNase_P 40 187 7 -RNase_P 41 187 1 -RNase_P 42 187 1 -RNase_P 43 187 1 -RNase_P 44 186 1 -RNase_P 44 187 1 -RNase_P 45 187 2 -RNase_P 48 186 1 -RNase_P 63 187 1 -RNase_P 64 187 1 -RNase_P 65 187 1 -RNase_P 81 187 2 -RNase_P 68 187 1 -RNase_P 82 187 2 -RNase_P 83 187 1 -RNase_P 85 187 2 -RNase_P 86 187 1 -RNase_P 89 147 1 -RNase_P 89 187 6 diff -r fb76303acf4f -r 431aebd93843 rna_probing/tool_dependencies.xml --- a/rna_probing/tool_dependencies.xml Wed Aug 05 09:12:07 2015 -0400 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,18 +0,0 @@ - - - - - - - - - - - - - - - - $REPOSITORY_INSTALL_DIR - - diff -r fb76303acf4f -r 431aebd93843 summarize_unique_barcodes.sh --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/summarize_unique_barcodes.sh Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,231 @@ +#!/bin/bash + +#################################################################################################### +#Copyright (C) 2014 Lukasz Kielpinski, Nikos Sidiropoulos + +#This program is free software: you can redistribute it and/or modify it under the terms of the +#GNU General Public License as published by the Free Software Foundation, either version 3 of the +#License, or (at your option) any later version. + +#This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without +#even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +#GNU General Public License for more details (http://www.gnu.org/licenses/). +#################################################################################################### + +function print_help { +cat < -b -p -t -k -r +End-of-message +exit +} + +#defaults +output_dir="output_dir" +trim_flag="False" + +#parse input +while getopts hf:b::p:o:ktr: myarg +do case "$myarg" in + h) print_help + exit ;; + f) bamfile="$OPTARG" ;; #required + b) barcodes="$OPTARG" ;; #optional + t) trim_flag="True" ;; + k) k2n="True" ;; + p) priming_pos="$OPTARG" ;; + o) output_dir="$OPTARG" ;; + r) R_SCRIPT_PATH="$OPTARG" ;; #required + [?]) echo "ERROR: Unknown parameter" + print_help + exit 1 ;; + esac +done + +###### Sanity checks ###### +if [ -z $bamfile ]; then + echo "Error: Aligned reads file is missing!" + print_help + exit 1 +fi + +if [ "$barcodes" == "None" ] && [ "$k2n" == "True" ]; then + echo "Error: k2n file cannot be produced without a barcode file!" + exit 1 +fi + + +mkdir -p $output_dir + +#Check if bamfile contains single or paired-end reads +samtools view -f 0x1 $bamfile | head -n 1 > paired + +if [ -s paired ]; then + #paired-end + samtools view $bamfile | awk 'BEGIN{OFS="\t"}{if(substr($0,1,1)!="@"){print}}' - | awk -v out="${output_dir}/trimming_stats.txt" -v flag="${trim_flag}" 'BEGIN{OFS="\t";counter[0]=0;counter[1]=0;counter[2]=0;counter[3]=0;counter[4]=0} + function abs(value){return(value<0?-value:value)} + function return_offset(local_offset){print($1, $3, $4+local_offset, $4+abs($9)-1);counter[local_offset]++} + ($2 != 99) {next}; + (flag == "False") {return_offset(0);next}; + (/[\s\t]MD:Z:/ && !/MD:Z:([012][ACGT])/) {return_offset(0);next}; + (/[\s\t]MD:Z:0[ACGT]/ && !/MD:Z:0[ACGT][01][ACGT]/ && substr($10,1,1)=="N") {return_offset(0);next}; + (/[\s\t]MD:Z:0[ACGT]/ && !/MD:Z:0[ACGT][01][ACGT]/) {return_offset(1);next}; + + (/[\s\t]MD:Z:1[ACGT]/ && !/MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(0);next}; + (/[\s\t]MD:Z:1[ACGT]/ && !/MD:Z:1[ACGT]0[ACGT]/) {return_offset(2);next}; + + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,1,2)=="NN") {return_offset(0);next}; + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,1,1)=="N") {return_offset(2);next}; + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(1);next}; + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(2);next}; + + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,2)=="NN") {return_offset(0);next}; + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(3);next}; + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,3,1)=="N") {return_offset(2);next}; + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/) {return_offset(3);next}; + + (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,3,1)=="N" && substr($10,1,1)=="N") {return_offset(0);next}; + (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,3,1)=="N") {return_offset(1);next}; + (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,1,1)=="N") {return_offset(3);next}; + (/MD:Z:0[ACGT]1[ACGT]/) {return_offset(3);next}; + + (/MD:Z:2[ACGT]/ && substr($10,3,1)=="N") {return_offset(0);next}; + (/MD:Z:2[ACGT]/) {return_offset(3);next}; + + (substr($10,3,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(3);next}; + (substr($10,2,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(2);next}; + (substr($10,1,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(1);next}; + + {return_offset(0);counter[4]++} + END{print("No trimming:",counter[0],", out of which not recognized MD field for:",counter[4],"; 1 nt trimmed:", counter[1],"; 2 nt trimmed:", counter[2],"; 3 nt trimmed:",counter[3]) > out}' | sort -S1G -k1,1 | gzip > positions_temp_sorted.gz + +else + #single-end + samtools view $bamfile | awk 'BEGIN{OFS="\t"}{if(substr($0,1,1)!="@"){print}}' - | awk -v out="${output_dir}/trimming_stats.txt" -v flag="${trim_flag}" 'BEGIN{OFS="\t";counter[0]=0;counter[1]=0;counter[2]=0;counter[3]=0;counter[4]=0} + function abs(value){return(value<0?-value:value)} + function return_offset(local_offset){print($1, $3, $4+local_offset, $4+abs($9)-1);counter[local_offset]++} + ($2 != 0) {next}; + (flag == "False") {return_offset(0);next}; + (/[\s\t]MD:Z:/ && !/MD:Z:([012][ACGT])/) {return_offset(0);next}; + (/[\s\t]MD:Z:0[ACGT]/ && !/MD:Z:0[ACGT][01][ACGT]/ && substr($10,1,1)=="N") {return_offset(0);next}; + (/[\s\t]MD:Z:0[ACGT]/ && !/MD:Z:0[ACGT][01][ACGT]/) {return_offset(1);next}; + + (/[\s\t]MD:Z:1[ACGT]/ && !/MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(0);next}; + (/[\s\t]MD:Z:1[ACGT]/ && !/MD:Z:1[ACGT]0[ACGT]/) {return_offset(2);next}; + + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,1,2)=="NN") {return_offset(0);next}; + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,1,1)=="N") {return_offset(2);next}; + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(1);next}; + (/MD:Z:0[ACGT]0[ACGT]/ && !/MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(2);next}; + + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,2)=="NN") {return_offset(0);next}; + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,2,1)=="N") {return_offset(3);next}; + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/ && substr($10,3,1)=="N") {return_offset(2);next}; + (/[\s\t]MD:Z:1[ACGT]0[ACGT]/) {return_offset(3);next}; + + (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,3,1)=="N" && substr($10,1,1)=="N") {return_offset(0);next}; + (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,3,1)=="N") {return_offset(1);next}; + (/MD:Z:0[ACGT]1[ACGT]/ && substr($10,1,1)=="N") {return_offset(3);next}; + (/MD:Z:0[ACGT]1[ACGT]/) {return_offset(3);next}; + + (/MD:Z:2[ACGT]/ && substr($10,3,1)=="N") {return_offset(0);next}; + (/MD:Z:2[ACGT]/) {return_offset(3);next}; + + (substr($10,3,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(3);next}; + (substr($10,2,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(2);next}; + (substr($10,1,1)!="N" && /MD:Z:0[ACGT]0[ACGT]0[ACGT]/) {return_offset(1);next}; + + {return_offset(0);counter[4]++} + END{print("No trimming:",counter[0],", out of which not recognized MD field for:",counter[4],"; 1 nt trimmed:", counter[1],"; 2 nt trimmed:", counter[2],"; 3 nt trimmed:",counter[3]) > out}' | sort -S1G -k1,1 | gzip > positions_temp_sorted.gz + +fi + +#Computing barcode length (Use the first line and compute the string length of the second column) +if [ "$barcodes" != "None" ]; then + + TMP=$(for line in `cut -f 2 $barcodes`; do if [ ! -z "$line" ]; then echo $line; break; fi; done) + BAR_LEN=`echo ${#TMP}` + + #Remove "@" from barcodes and sort them + sed 's/^.//' $barcodes | sort -k1,1 -S1G | gzip > barcodes_temp_sorted.gz + + #Merge poistions and barcodes + join -1 1 <(zcat positions_temp_sorted.gz) <(zcat barcodes_temp_sorted.gz) | cut -f 2,3,4,5 -d " " | awk '{if($4 !~ /N/){print}}' | awk -v bar_len="${BAR_LEN}" '{if(length($4)==bar_len){print}}' | gzip > merged_temp.gz + + rm barcodes_temp_sorted.gz + +else + zcat positions_temp_sorted.gz | cut -f 2,3,4 | gzip > merged_temp.gz +fi + +#If the experiment is single-end set NA values to the priming column. +if [ ! -s paired ]; then + zcat merged_temp.gz | awk '{print $1, $2, "NA", $4, $5}' - > merged_temp2 + gzip -c merged_temp2 > merged_temp.gz + rm merged_temp2 +fi + +#Fix priming position +if [ ! -z $priming_pos ]; then + zcat merged_temp.gz | awk -v pos="${priming_pos}" '{print $1, $2, pos, $4, $5}' - > merged_temp2 + gzip -c merged_temp2 > merged_temp.gz + rm merged_temp2 +fi + +#File summary.txt columns: RNA_ID, Start, End, barcode sequence, sequenced_count[=number of sequenced fragments fulfilling previous requiremnts] + +zcat merged_temp.gz | awk '{barcode[$1][$2][$3][$4]++}END{ +for(RNA in barcode){ +for(start_position in barcode[RNA]){ +for(end_position in barcode[RNA][start_position]){ +for(barseq in barcode[RNA][start_position][end_position]){print RNA,start_position,end_position,barseq,barcode[RNA][start_position][end_position][barseq]}}}}}' > $output_dir/summary.txt + +#File unique_barcodes.txt columns: RNA_ID, Start, End, number of unique barcodes observed for this fragment. + +awk '{barcode[$1][$2][$3]++}END{ +for(RNA in barcode){ +for(start_position in barcode[RNA]){ +for(end_position in barcode[RNA][start_position]){print RNA "\t" start_position "\t" end_position "\t" barcode[RNA][start_position][end_position]}}}}' $output_dir/summary.txt > $output_dir/unique_barcodes.txt & + +#File read_counts.txt colums: RNA_ID, Start, End, sequenced_count + +zcat merged_temp.gz | awk '{barcode[$1][$2][$3]++}END{ +for(RNA in barcode){ +for(start_position in barcode[RNA]){ +for(end_position in barcode[RNA][start_position]){print RNA "\t" start_position "\t" end_position "\t" barcode[RNA][start_position][end_position]}}}}' > $output_dir/read_counts.txt & + +wait + +#Print the maximum observed barcodes value. Usefull to assess the necessity of producing the k2n file. +if [ "$barcodes" != "None" ]; then + + cut -f 4 $output_dir/unique_barcodes.txt | sort -S1G -rn > sorted_bars + max_observed_barcodes=`head -n 1 sorted_bars` + + echo "Maximum observed Barcodes = ${max_observed_barcodes}" + rm sorted_bars +fi + +#Produce k2n file +if [ "$k2n" == "True" ]; then + if [ ! -s paired ]; then + Rscript $R_SCRIPT_PATH/k2n.R merged_temp.gz $output_dir/unique_barcodes.txt $output_dir/k2n.txt FALSE + else + Rscript $R_SCRIPT_PATH/k2n.R merged_temp.gz $output_dir/unique_barcodes.txt $output_dir/k2n.txt TRUE + fi +fi + +#Remove temp files +rm merged_temp.gz positions_temp_sorted.gz paired diff -r fb76303acf4f -r 431aebd93843 summarize_unique_barcodes.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/summarize_unique_barcodes.xml Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,139 @@ + + + + + gnu_awk + samtools + R_3_1_1 + RNAprobR + RNAprobR + RNA_PROBING_SCRIPT_PATH + + + + summarize_unique_barcodes.sh + + ## Inputs + -f $input1 -b $input2 + + ## + + #if str( $k2n ) == 'True': + -k + #end if + + #if str( $priming.flag ) == 'True': + -p $priming.position + #end if + + #if str( $trimming ) == 'True': + -t + #end if + + -r \$RNA_PROBING_SCRIPT_PATH + + + + + + + + + + + + + + + + + + + + + + + + + + + trimming is True + + + input2 != None + + + + k2n is True + + + + + + + + + + + + + + + + +**What it does** + +*Summarize Unique Barcodes* counts the number of unique random barcodes and reads associated with each sequenced fragment. A fragment is understood as 1) a pair of Reverse Transcriptase (RT) termination site and RT priming site given paired end sequencing, or 2) an RT termination site in a single end sequencing. For non-barcoded sequencing it only counts the reads matching each fragment. + +------ + +**Inputs** + +*Summarize Unique Barcodes* requires a file containing the Aligned Reads (required) in BAM_ format and tabular file with the Barcodes (optional) produced by the *Preprocessing* tool of the *RNA probing* suite. + +.. _BAM: http://samtools.github.io/hts-specs/SAMv1.pdf + +------- + +**Parameters** + +**Produce k2n file** - A file that contains a sequence of numbers where the n-th element informs how many unique cDNA molecules gives rise to observing n unique barcodes in a given sample. Required for calculating Estimated Unique Counts (EUCs) in tool *Normalize*. + +**Trim untemplated nucleotides** - Untemplated nucleotides can be added to cDNA 3’ ends via terminal transferase activity of reverse transcriptase which offset the location of the read-end mapping and lead to erroneous assignment of reactivity information to nucleotides upstream of those which has reacted (Schmidt and Mueller, 1999, Talkish et al., 2014). Setting this parameter on will remove those nucleotides. + +Recommended for methods based on detecting reverse transcription termination sites (e.g. DMS-Seq, HRF-Seq or SHAPE-Seq), and not for methods based on ligating the linker directly to RNA (e.g. PARS or FragSeq). + +**Set priming position** - Applicable when the priming site is fixed. + +------ + +**Outputs** + +**Unique Barcodes** (if a Barcode file is given) is a tabular file with 4 columns. + +====== ========================================================== +Column Description +------ ---------------------------------------------------------- + 1 Transcript identifier + 2 RT termination site (start) + 3 RT priming site (end) + 4 Count of unique barcodes associated with fragments matching the first three columns +====== ========================================================== + +. + +**Read Counts** is similar to Unique Barcodes but the fourth column is a count of reads matching first three columns. + +**k2n file** as described above. + +**Trimming Stats** reports statistics of trimming untemplated nucleotides from read ends. + + + + + 10.1093/nar/gku167 + 10.1093/nar/27.21.e31-i + 10.1261/rna.042218.113 + + + diff -r fb76303acf4f -r 431aebd93843 test-data/RNase_P.fa --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/RNase_P.fa Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,4 @@ +>RNase_P +GGGGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATAT +GGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCG +ATCCGCTTCGGCGGATCCAAATCGGGCTTCGGTCCGGTTCAGATCGGAAGAGCACACGTCT diff -r fb76303acf4f -r 431aebd93843 test-data/aligned.bam Binary file test-data/aligned.bam has changed diff -r fb76303acf4f -r 431aebd93843 test-data/barcodes.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/barcodes.txt Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,671 @@ +@HWI-ST575:165:C1P5BACXX:1:1101:1666:1132 TCCATCC +@HWI-ST575:165:C1P5BACXX:1:1101:2239:1101 CAACCCC +@HWI-ST575:165:C1P5BACXX:1:1101:2455:1083 CCACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:3207:1238 TGCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:3405:1152 TCAATAC +@HWI-ST575:165:C1P5BACXX:1:1101:3512:1179 TTTCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:4473:1182 GGCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:5162:1092 CTTCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:6810:1177 CGATCAT +@HWI-ST575:165:C1P5BACXX:1:1101:8268:1157 CAAACGG +@HWI-ST575:165:C1P5BACXX:1:1101:8740:1185 GTATGAC +@HWI-ST575:165:C1P5BACXX:1:1101:12414:1099 ATCGGGC +@HWI-ST575:165:C1P5BACXX:1:1101:14053:1112 TATTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:14219:1152 TCGTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15012:1211 GCCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15774:1231 ATTACAC +@HWI-ST575:165:C1P5BACXX:1:1101:17047:1127 AATGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:18461:1188 CGATCAC +@HWI-ST575:165:C1P5BACXX:1:1101:19911:1138 TACCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:1311:1500 ACTAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:2110:1396 CCCCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:2970:1430 AGGCGAC +@HWI-ST575:165:C1P5BACXX:1:1101:4079:1444 CTGGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:5495:1254 TCGAACT +@HWI-ST575:165:C1P5BACXX:1:1101:5684:1314 TAATCCT +@HWI-ST575:165:C1P5BACXX:1:1101:6002:1450 GTTGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:6163:1455 TCAATGG +@HWI-ST575:165:C1P5BACXX:1:1101:6370:1342 CAGGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:7500:1303 ACTAAAA +@HWI-ST575:165:C1P5BACXX:1:1101:8186:1308 ATCCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:10049:1341 GATCTGC +@HWI-ST575:165:C1P5BACXX:1:1101:10737:1349 AAGCGAC +@HWI-ST575:165:C1P5BACXX:1:1101:11993:1308 CTGACCC +@HWI-ST575:165:C1P5BACXX:1:1101:12260:1420 TCGGTCG +@HWI-ST575:165:C1P5BACXX:1:1101:13041:1365 CAACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:13915:1290 TCACCCT +@HWI-ST575:165:C1P5BACXX:1:1101:13944:1432 GCAGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:14362:1371 CATCGCA +@HWI-ST575:165:C1P5BACXX:1:1101:14865:1283 CATCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:15341:1459 AACCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15856:1385 CGATCAC +@HWI-ST575:165:C1P5BACXX:1:1101:16168:1446 CCTATAC +@HWI-ST575:165:C1P5BACXX:1:1101:16274:1448 GACATAC +@HWI-ST575:165:C1P5BACXX:1:1101:17532:1255 GTCAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:19852:1368 ACTCATG +@HWI-ST575:165:C1P5BACXX:1:1101:20338:1414 AACTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:1782:1682 ACTGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:2733:1718 TGCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:2796:1586 TCACACT +@HWI-ST575:165:C1P5BACXX:1:1101:3075:1523 ACTAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:4251:1515 AATACAC +@HWI-ST575:165:C1P5BACXX:1:1101:4481:1718 CATCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:4978:1615 GACGGCC +@HWI-ST575:165:C1P5BACXX:1:1101:6935:1687 AAACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:7393:1637 TGCAGGA +@HWI-ST575:165:C1P5BACXX:1:1101:7744:1585 ACCTTTC +@HWI-ST575:165:C1P5BACXX:1:1101:7646:1624 CTTGTGT +@HWI-ST575:165:C1P5BACXX:1:1101:8080:1642 TTTGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:8452:1710 TCTAGTG +@HWI-ST575:165:C1P5BACXX:1:1101:8575:1513 GAGGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:8610:1649 TCTTACC +@HWI-ST575:165:C1P5BACXX:1:1101:9515:1701 TCCGCCC +@HWI-ST575:165:C1P5BACXX:1:1101:9782:1735 TTACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:10892:1546 CCATTCT +@HWI-ST575:165:C1P5BACXX:1:1101:10875:1631 ATTTGGT +@HWI-ST575:165:C1P5BACXX:1:1101:11536:1555 TTGATGC +@HWI-ST575:165:C1P5BACXX:1:1101:11540:1624 TGGCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:12292:1617 GGAAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:13423:1569 CATCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:13576:1612 CACGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14491:1526 TGACCAT +@HWI-ST575:165:C1P5BACXX:1:1101:15109:1589 CACTCCT +@HWI-ST575:165:C1P5BACXX:1:1101:16270:1554 TTGATGC +@HWI-ST575:165:C1P5BACXX:1:1101:17059:1656 GGACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:17823:1711 TCCTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:18750:1719 CATATAC +@HWI-ST575:165:C1P5BACXX:1:1101:19397:1535 ATACTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19270:1553 CTGCCAA +@HWI-ST575:165:C1P5BACXX:1:1101:20160:1653 ACACGAC +@HWI-ST575:165:C1P5BACXX:1:1101:20348:1677 GCATCAC +@HWI-ST575:165:C1P5BACXX:1:1101:1152:1999 TCCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:1575:1782 CCCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:2146:1765 TAATGAC +@HWI-ST575:165:C1P5BACXX:1:1101:2817:1860 CATTGAA +@HWI-ST575:165:C1P5BACXX:1:1101:4121:1770 GCCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:4015:1808 TTCGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:4671:1789 TCGACAC +@HWI-ST575:165:C1P5BACXX:1:1101:5190:1986 TCGAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:7320:1866 AAACTAC +@HWI-ST575:165:C1P5BACXX:1:1101:7508:1798 GCTAGTT +@HWI-ST575:165:C1P5BACXX:1:1101:9647:1841 TTCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:11078:1760 CGCACTC +@HWI-ST575:165:C1P5BACXX:1:1101:12091:1910 TTACGCA +@HWI-ST575:165:C1P5BACXX:1:1101:13182:1885 ACTGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:13475:1836 AATCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:14275:1813 AGCTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:14914:1754 GCTGTCA +@HWI-ST575:165:C1P5BACXX:1:1101:15826:1814 CAGACCT +@HWI-ST575:165:C1P5BACXX:1:1101:16316:1995 GGGATTC +@HWI-ST575:165:C1P5BACXX:1:1101:17677:1880 CCAAACC +@HWI-ST575:165:C1P5BACXX:1:1101:18640:1881 AACGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:18890:1897 AGGCAGC +@HWI-ST575:165:C1P5BACXX:1:1101:1102:2244 GCCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:2310:2103 GTCTACC +@HWI-ST575:165:C1P5BACXX:1:1101:3289:2146 TACTATT +@HWI-ST575:165:C1P5BACXX:1:1101:4895:2080 TACGAGC +@HWI-ST575:165:C1P5BACXX:1:1101:4932:2108 TATCAAG +@HWI-ST575:165:C1P5BACXX:1:1101:5806:2073 TCCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:6214:2065 GCAAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:6143:2137 TCCACAC +@HWI-ST575:165:C1P5BACXX:1:1101:6241:2199 CGGTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:7894:2030 ATTGTGC +@HWI-ST575:165:C1P5BACXX:1:1101:8072:2245 CCACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8299:2087 ATACCCC +@HWI-ST575:165:C1P5BACXX:1:1101:8471:2183 ACACATT +@HWI-ST575:165:C1P5BACXX:1:1101:9051:2011 CGGTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:11298:2099 ATTACCC +@HWI-ST575:165:C1P5BACXX:1:1101:11886:2227 AGGTCCC +@HWI-ST575:165:C1P5BACXX:1:1101:12469:2156 CCCGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:13307:2211 GCGGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:13967:2112 CACCATT +@HWI-ST575:165:C1P5BACXX:1:1101:14357:2064 AACCAAA +@HWI-ST575:165:C1P5BACXX:1:1101:14498:2106 TGACTAC +@HWI-ST575:165:C1P5BACXX:1:1101:14632:2166 GGGTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:14873:2011 CCTTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15054:2152 GCCGGCC +@HWI-ST575:165:C1P5BACXX:1:1101:15855:2031 TCACCAA +@HWI-ST575:165:C1P5BACXX:1:1101:15913:2171 CACCATG +@HWI-ST575:165:C1P5BACXX:1:1101:16436:2126 CACCACC +@HWI-ST575:165:C1P5BACXX:1:1101:16535:2143 CAGGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:16829:2249 CACCATC +@HWI-ST575:165:C1P5BACXX:1:1101:17455:2003 TGGGTCC +@HWI-ST575:165:C1P5BACXX:1:1101:17878:2158 TTCAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:18041:2200 CAGAACT +@HWI-ST575:165:C1P5BACXX:1:1101:2284:2369 CAGACAC +@HWI-ST575:165:C1P5BACXX:1:1101:2836:2439 TCTCTGC +@HWI-ST575:165:C1P5BACXX:1:1101:4292:2260 GACACTT +@HWI-ST575:165:C1P5BACXX:1:1101:4629:2330 TAATCGT +@HWI-ST575:165:C1P5BACXX:1:1101:4537:2454 TGGCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:5316:2437 GTTCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:5705:2286 TAAACAC +@HWI-ST575:165:C1P5BACXX:1:1101:6181:2354 TGCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:6640:2254 GCATCCG +@HWI-ST575:165:C1P5BACXX:1:1101:6520:2397 ACCCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:7019:2438 GATCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:8048:2260 TATTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:8522:2464 GCCCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:8845:2462 GCGGCGG +@HWI-ST575:165:C1P5BACXX:1:1101:9068:2302 ACAACAC +@HWI-ST575:165:C1P5BACXX:1:1101:11110:2418 ATGACTC +@HWI-ST575:165:C1P5BACXX:1:1101:11986:2397 AATCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:12227:2444 TATGACC +@HWI-ST575:165:C1P5BACXX:1:1101:12315:2285 GATGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:12492:2350 ACCAGAT +@HWI-ST575:165:C1P5BACXX:1:1101:13583:2475 CTCAAGC +@HWI-ST575:165:C1P5BACXX:1:1101:14469:2354 ACAAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14989:2278 GCCATTC +@HWI-ST575:165:C1P5BACXX:1:1101:15188:2338 ACCTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:15331:2257 CACACTT +@HWI-ST575:165:C1P5BACXX:1:1101:15849:2368 ACCGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:16152:2410 TAGCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:16935:2365 GCTCTCC +@HWI-ST575:165:C1P5BACXX:1:1101:17289:2412 CGACCTC +@HWI-ST575:165:C1P5BACXX:1:1101:18384:2478 ACGGACT +@HWI-ST575:165:C1P5BACXX:1:1101:19068:2414 GTGCTAT +@HWI-ST575:165:C1P5BACXX:1:1101:19461:2478 TACTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19502:2351 GCCAATC +@HWI-ST575:165:C1P5BACXX:1:1101:19741:2372 GCTGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:19565:2447 TAACACC +@HWI-ST575:165:C1P5BACXX:1:1101:20585:2479 CCTGACT +@HWI-ST575:165:C1P5BACXX:1:1101:20888:2259 TGCAAGC +@HWI-ST575:165:C1P5BACXX:1:1101:1310:2696 CAGCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:4969:2645 CCTTAAT +@HWI-ST575:165:C1P5BACXX:1:1101:6485:2694 AAGCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:6896:2699 CCAATAC +@HWI-ST575:165:C1P5BACXX:1:1101:7108:2513 CAACGAC +@HWI-ST575:165:C1P5BACXX:1:1101:7123:2615 TGTTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:7199:2618 TTAATCC +@HWI-ST575:165:C1P5BACXX:1:1101:10015:2714 CGGACTC +@HWI-ST575:165:C1P5BACXX:1:1101:10105:2714 GCTGGTC +@HWI-ST575:165:C1P5BACXX:1:1101:11341:2736 TCTTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:11611:2512 AGACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:11611:2576 GGCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:11522:2633 CACTATC +@HWI-ST575:165:C1P5BACXX:1:1101:12762:2599 ATCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:13122:2666 AACCGCA +@HWI-ST575:165:C1P5BACXX:1:1101:13331:2504 CTAGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:13328:2614 TAAGCGC +@HWI-ST575:165:C1P5BACXX:1:1101:13944:2746 CCAAACC +@HWI-ST575:165:C1P5BACXX:1:1101:14544:2676 CCTCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:16211:2706 TCATTAC +@HWI-ST575:165:C1P5BACXX:1:1101:17293:2515 CTTACAT +@HWI-ST575:165:C1P5BACXX:1:1101:17341:2572 GAATGGT +@HWI-ST575:165:C1P5BACXX:1:1101:17813:2676 ACACATT +@HWI-ST575:165:C1P5BACXX:1:1101:18322:2589 CCACTCC +@HWI-ST575:165:C1P5BACXX:1:1101:18563:2613 TGCACTC +@HWI-ST575:165:C1P5BACXX:1:1101:19030:2538 ATACCCC +@HWI-ST575:165:C1P5BACXX:1:1101:19375:2502 GTAGTCC +@HWI-ST575:165:C1P5BACXX:1:1101:19630:2588 ACAACCC +@HWI-ST575:165:C1P5BACXX:1:1101:1885:2921 ACGGACC +@HWI-ST575:165:C1P5BACXX:1:1101:3712:2816 GCTTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:3931:2939 TGTTCGG +@HWI-ST575:165:C1P5BACXX:1:1101:4575:2908 AAACTCC +@HWI-ST575:165:C1P5BACXX:1:1101:4670:2972 TTGTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:4779:2823 ACTCGAC +@HWI-ST575:165:C1P5BACXX:1:1101:4804:2998 GTTACTG +@HWI-ST575:165:C1P5BACXX:1:1101:5202:2792 CGCACGC +@HWI-ST575:165:C1P5BACXX:1:1101:5939:2936 CTTGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:6091:2918 CGACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:6392:2828 CGCGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:7180:2991 CATCGGG +@HWI-ST575:165:C1P5BACXX:1:1101:7419:2920 CGTACAC +@HWI-ST575:165:C1P5BACXX:1:1101:7772:2979 GCACCTC +@HWI-ST575:165:C1P5BACXX:1:1101:8507:2811 CGCCGCC +@HWI-ST575:165:C1P5BACXX:1:1101:8832:2869 GCCTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:9485:2898 GCTTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:9990:2821 CATGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:10328:2966 ATCGGTC +@HWI-ST575:165:C1P5BACXX:1:1101:11508:2796 AACGGTC +@HWI-ST575:165:C1P5BACXX:1:1101:11726:2972 GGAGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:12044:2834 CCATGAA +@HWI-ST575:165:C1P5BACXX:1:1101:12169:2963 ACTCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:13168:2760 CGACCTC +@HWI-ST575:165:C1P5BACXX:1:1101:13484:2768 TTTTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:13399:2774 CTAGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:13467:2781 GCATCTC +@HWI-ST575:165:C1P5BACXX:1:1101:13716:2837 CGCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:14400:2843 CAATTTT +@HWI-ST575:165:C1P5BACXX:1:1101:14816:2802 CACTGTT +@HWI-ST575:165:C1P5BACXX:1:1101:15055:2926 CTAGACG +@HWI-ST575:165:C1P5BACXX:1:1101:17639:2908 TACAGAT +@HWI-ST575:165:C1P5BACXX:1:1101:20319:2983 GGTATTT +@HWI-ST575:165:C1P5BACXX:1:1101:20783:2769 AAAACGC +@HWI-ST575:165:C1P5BACXX:1:1101:1673:3017 AATATCC +@HWI-ST575:165:C1P5BACXX:1:1101:2153:3048 ATTCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:4715:3184 TCCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:4796:3234 CAACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:5104:3008 ACGGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:5272:3008 GAAGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:5594:3119 ACCCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:6927:3052 CACAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:7098:3130 AGCCTCC +@HWI-ST575:165:C1P5BACXX:1:1101:7842:3018 AACCACT +@HWI-ST575:165:C1P5BACXX:1:1101:8316:3056 CTCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:8582:3236 CCAAAGC +@HWI-ST575:165:C1P5BACXX:1:1101:10343:3076 TTCATAC +@HWI-ST575:165:C1P5BACXX:1:1101:10433:3184 CCCTTTT +@HWI-ST575:165:C1P5BACXX:1:1101:10787:3078 AGCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:10951:3116 GGTCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:11196:3016 TGCTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:11257:3175 TTTTGGT +@HWI-ST575:165:C1P5BACXX:1:1101:12061:3148 CCAAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:12768:3208 ATTCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14168:3032 TCCCCTC +@HWI-ST575:165:C1P5BACXX:1:1101:14033:3072 GCAACGG +@HWI-ST575:165:C1P5BACXX:1:1101:14598:3012 TGTTTTC +@HWI-ST575:165:C1P5BACXX:1:1101:14516:3084 GACGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15426:3038 ACAAGTG +@HWI-ST575:165:C1P5BACXX:1:1101:16490:3172 GCATTCG +@HWI-ST575:165:C1P5BACXX:1:1101:16810:3009 AGTTAGC +@HWI-ST575:165:C1P5BACXX:1:1101:17555:3239 GCTATAC +@HWI-ST575:165:C1P5BACXX:1:1101:18319:3009 ATGCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19255:3046 CAATAAC +@HWI-ST575:165:C1P5BACXX:1:1101:20168:3013 GACTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:21025:3228 TATAATG +@HWI-ST575:165:C1P5BACXX:1:1101:3940:3277 TCACGGC +@HWI-ST575:165:C1P5BACXX:1:1101:5732:3431 CAATATT +@HWI-ST575:165:C1P5BACXX:1:1101:6498:3306 AGCTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:6927:3383 ATACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8668:3366 AACCCTC +@HWI-ST575:165:C1P5BACXX:1:1101:9555:3271 TCGCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:9960:3491 ATATCGC +@HWI-ST575:165:C1P5BACXX:1:1101:11371:3324 TTCGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:11999:3288 CCATATC +@HWI-ST575:165:C1P5BACXX:1:1101:12147:3289 TCCAACG +@HWI-ST575:165:C1P5BACXX:1:1101:12666:3312 TTACATG +@HWI-ST575:165:C1P5BACXX:1:1101:12701:3456 CCAATCC +@HWI-ST575:165:C1P5BACXX:1:1101:13062:3317 TTTCTCC +@HWI-ST575:165:C1P5BACXX:1:1101:13545:3337 CAACTAC +@HWI-ST575:165:C1P5BACXX:1:1101:14345:3429 CCAATAT +@HWI-ST575:165:C1P5BACXX:1:1101:14585:3422 AGATTAC +@HWI-ST575:165:C1P5BACXX:1:1101:16628:3381 TTCCCTC +@HWI-ST575:165:C1P5BACXX:1:1101:1080:3578 TACGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:1525:3749 TGCGGCC +@HWI-ST575:165:C1P5BACXX:1:1101:2431:3630 AACCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:2802:3618 TGACTTT +@HWI-ST575:165:C1P5BACXX:1:1101:3064:3520 CCTGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:3610:3614 GCGCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:4225:3617 TCGAGCT +@HWI-ST575:165:C1P5BACXX:1:1101:4165:3628 ACCATAC +@HWI-ST575:165:C1P5BACXX:1:1101:4951:3655 CGACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:4878:3725 TACATAT +@HWI-ST575:165:C1P5BACXX:1:1101:5530:3596 CTCGTTT +@HWI-ST575:165:C1P5BACXX:1:1101:6120:3600 ACAACAC +@HWI-ST575:165:C1P5BACXX:1:1101:6900:3547 CAAGCAT +@HWI-ST575:165:C1P5BACXX:1:1101:7633:3566 CAACACT +@HWI-ST575:165:C1P5BACXX:1:1101:9213:3506 TCCCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:10414:3701 GCAATTC +@HWI-ST575:165:C1P5BACXX:1:1101:10901:3561 GCTACCT +@HWI-ST575:165:C1P5BACXX:1:1101:10915:3580 CTGTACA +@HWI-ST575:165:C1P5BACXX:1:1101:11184:3661 CCTATTC +@HWI-ST575:165:C1P5BACXX:1:1101:11415:3524 CTAGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:12086:3581 TCCATCC +@HWI-ST575:165:C1P5BACXX:1:1101:12420:3632 ACCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:13315:3747 TCAGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14374:3673 CATGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:14502:3661 ACACAGC +@HWI-ST575:165:C1P5BACXX:1:1101:14952:3691 CATCATT +@HWI-ST575:165:C1P5BACXX:1:1101:15058:3596 AGGCTGA +@HWI-ST575:165:C1P5BACXX:1:1101:17467:3686 ACCAAAT +@HWI-ST575:165:C1P5BACXX:1:1101:17669:3607 AGCCCGG +@HWI-ST575:165:C1P5BACXX:1:1101:18356:3623 CCAGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:18913:3633 TCTGAAT +@HWI-ST575:165:C1P5BACXX:1:1101:20863:3620 CCTGTAA +@HWI-ST575:165:C1P5BACXX:1:1101:1773:3939 GTGACAC +@HWI-ST575:165:C1P5BACXX:1:1101:2081:3878 TGTGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:4430:3860 GAGCCTT +@HWI-ST575:165:C1P5BACXX:1:1101:6400:3801 AGTCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:6719:3898 TGACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:8167:3757 AGAGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:9714:3894 CCATACC +@HWI-ST575:165:C1P5BACXX:1:1101:11290:3835 CTGCGGT +@HWI-ST575:165:C1P5BACXX:1:1101:12038:3902 GTAGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:12700:3779 CTTACTC +@HWI-ST575:165:C1P5BACXX:1:1101:12790:3907 GCTTCTT +@HWI-ST575:165:C1P5BACXX:1:1101:13831:3779 TACATAC +@HWI-ST575:165:C1P5BACXX:1:1101:14731:3871 ACGCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14772:3935 AGCACAC +@HWI-ST575:165:C1P5BACXX:1:1101:15067:3763 GCCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15156:3801 CTCCGTC +@HWI-ST575:165:C1P5BACXX:1:1101:16559:3892 GCCGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:17255:3956 GCTCATT +@HWI-ST575:165:C1P5BACXX:1:1101:17500:3790 GCGACGG +@HWI-ST575:165:C1P5BACXX:1:1101:18440:3926 GAGGTTT +@HWI-ST575:165:C1P5BACXX:1:1101:19111:3987 TACCACC +@HWI-ST575:165:C1P5BACXX:1:1101:19557:3888 CACGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19920:3801 GGGCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:1580:4241 TTCAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:2986:4163 TCGAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:4437:4204 AACCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:5319:4088 GGTTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:5702:4094 CTATATC +@HWI-ST575:165:C1P5BACXX:1:1101:5824:4111 CATCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:6984:4013 ACCGACT +@HWI-ST575:165:C1P5BACXX:1:1101:7890:4076 CCCACAC +@HWI-ST575:165:C1P5BACXX:1:1101:8052:4159 TTTTCGG +@HWI-ST575:165:C1P5BACXX:1:1101:8080:4229 TCAATCT +@HWI-ST575:165:C1P5BACXX:1:1101:8526:4228 CATTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8765:4049 TTCCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:9822:4042 TCTTCAT +@HWI-ST575:165:C1P5BACXX:1:1101:10184:4066 TAAAGCG +@HWI-ST575:165:C1P5BACXX:1:1101:11794:4113 CTTTATC +@HWI-ST575:165:C1P5BACXX:1:1101:12356:4148 CGTTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:14554:4015 ACGTATC +@HWI-ST575:165:C1P5BACXX:1:1101:14736:4035 ATAGCAG +@HWI-ST575:165:C1P5BACXX:1:1101:15267:4200 GATACTC +@HWI-ST575:165:C1P5BACXX:1:1101:17040:4246 TGATTAC +@HWI-ST575:165:C1P5BACXX:1:1101:17659:4106 CTGGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:17880:4233 TTCAATC +@HWI-ST575:165:C1P5BACXX:1:1101:19348:4134 GATCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:1929:4262 GGCCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:3262:4389 GCTTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:3996:4500 GCCAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:4255:4286 TTCTCCT +@HWI-ST575:165:C1P5BACXX:1:1101:4583:4479 CACCATC +@HWI-ST575:165:C1P5BACXX:1:1101:5886:4450 AACATCT +@HWI-ST575:165:C1P5BACXX:1:1101:6699:4365 CACGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:7054:4268 CCATTAC +@HWI-ST575:165:C1P5BACXX:1:1101:7021:4304 GAAGCTG +@HWI-ST575:165:C1P5BACXX:1:1101:7330:4272 GGCCGAC +@HWI-ST575:165:C1P5BACXX:1:1101:7985:4412 AACTCCC +@HWI-ST575:165:C1P5BACXX:1:1101:9154:4399 CGTACAC +@HWI-ST575:165:C1P5BACXX:1:1101:9397:4390 TTAGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:9626:4444 CATCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:9920:4375 ATGTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:9928:4484 ACACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:10347:4290 TCCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:10607:4474 ACCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:11109:4422 ATTTTTT +@HWI-ST575:165:C1P5BACXX:1:1101:11719:4366 CTTGGAC +@HWI-ST575:165:C1P5BACXX:1:1101:11803:4357 CAGTAGC +@HWI-ST575:165:C1P5BACXX:1:1101:12622:4263 ACGTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:13888:4376 TGAGCCC +@HWI-ST575:165:C1P5BACXX:1:1101:14985:4327 TAAGCCC +@HWI-ST575:165:C1P5BACXX:1:1101:15294:4416 CACGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:16020:4286 CCTCTCG +@HWI-ST575:165:C1P5BACXX:1:1101:16371:4398 GGTCGTG +@HWI-ST575:165:C1P5BACXX:1:1101:17919:4354 ACCAGTG +@HWI-ST575:165:C1P5BACXX:1:1101:19347:4445 TATGACT +@HWI-ST575:165:C1P5BACXX:1:1101:19620:4333 CTACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:20453:4255 TCACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:20959:4324 ATTCATT +@HWI-ST575:165:C1P5BACXX:1:1101:20762:4415 CTTCGCT +@HWI-ST575:165:C1P5BACXX:1:1101:21156:4355 TCGACCC +@HWI-ST575:165:C1P5BACXX:1:1101:21005:4492 AGACTAC +@HWI-ST575:165:C1P5BACXX:1:1101:2208:4721 ACCACAC +@HWI-ST575:165:C1P5BACXX:1:1101:3594:4541 CCACTGC +@HWI-ST575:165:C1P5BACXX:1:1101:4453:4680 CTAACGG +@HWI-ST575:165:C1P5BACXX:1:1101:4574:4514 TACGGCC +@HWI-ST575:165:C1P5BACXX:1:1101:4512:4554 ATCCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:6091:4740 CCTGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:7755:4724 TCATGAC +@HWI-ST575:165:C1P5BACXX:1:1101:10497:4685 CGACCGC +@HWI-ST575:165:C1P5BACXX:1:1101:13319:4694 CATGTCC +@HWI-ST575:165:C1P5BACXX:1:1101:14064:4581 CTGACAC +@HWI-ST575:165:C1P5BACXX:1:1101:14735:4724 GGGAACC +@HWI-ST575:165:C1P5BACXX:1:1101:15633:4606 ATGAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:16660:4702 ATTAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:18593:4710 TTCCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19241:4545 AGACGAC +@HWI-ST575:165:C1P5BACXX:1:1101:19601:4684 GCAATCT +@HWI-ST575:165:C1P5BACXX:1:1101:20709:4735 TCACTTC +@HWI-ST575:165:C1P5BACXX:1:1101:21224:4560 ACTTGAA +@HWI-ST575:165:C1P5BACXX:1:1101:2354:4948 TTACGAC +@HWI-ST575:165:C1P5BACXX:1:1101:2921:4941 CGCGCAT +@HWI-ST575:165:C1P5BACXX:1:1101:4132:4919 ACGACCC +@HWI-ST575:165:C1P5BACXX:1:1101:5510:4838 CGTATAC +@HWI-ST575:165:C1P5BACXX:1:1101:6248:4986 ACAAAGC +@HWI-ST575:165:C1P5BACXX:1:1101:6936:4836 CCCTGTC +@HWI-ST575:165:C1P5BACXX:1:1101:7709:4867 ACGTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:9439:4978 ACGTCCC +@HWI-ST575:165:C1P5BACXX:1:1101:9572:4907 TCAACAC +@HWI-ST575:165:C1P5BACXX:1:1101:9900:4892 CGTTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:9950:4989 CAGTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:10290:4901 TATGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:10794:4755 ATAGCCT +@HWI-ST575:165:C1P5BACXX:1:1101:11203:4970 ATCTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:11597:4816 CTAATAC +@HWI-ST575:165:C1P5BACXX:1:1101:11814:4971 GTTGGCT +@HWI-ST575:165:C1P5BACXX:1:1101:12815:4804 GTCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15064:4795 TTACCGA +@HWI-ST575:165:C1P5BACXX:1:1101:15338:4874 TCCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:15739:4984 CCCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:16043:4995 CATAACG +@HWI-ST575:165:C1P5BACXX:1:1101:17608:4859 ACGCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:17879:4904 GTTAATC +@HWI-ST575:165:C1P5BACXX:1:1101:18072:4949 CTTAACT +@HWI-ST575:165:C1P5BACXX:1:1101:18453:4916 GGGACAC +@HWI-ST575:165:C1P5BACXX:1:1101:19254:4780 GCCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:19861:4798 ATTGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19870:4937 CAACTCC +@HWI-ST575:165:C1P5BACXX:1:1101:19796:4989 ACCCGAC +@HWI-ST575:165:C1P5BACXX:1:1101:20053:4972 TATGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:20782:4908 CGACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:1189:5021 ACCGGAT +@HWI-ST575:165:C1P5BACXX:1:1101:1686:5115 TCATAGC +@HWI-ST575:165:C1P5BACXX:1:1101:2938:5127 GCCTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:3405:5036 ACTGATC +@HWI-ST575:165:C1P5BACXX:1:1101:7045:5134 ATGGCCT +@HWI-ST575:165:C1P5BACXX:1:1101:7123:5216 CGCCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:8358:5145 CTGCGGC +@HWI-ST575:165:C1P5BACXX:1:1101:8840:5173 TACGCCG +@HWI-ST575:165:C1P5BACXX:1:1101:9731:5160 AAGGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:10830:5146 GAGCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:11032:5068 GCGCAGG +@HWI-ST575:165:C1P5BACXX:1:1101:11565:5085 GCACCTC +@HWI-ST575:165:C1P5BACXX:1:1101:12209:5017 ACACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:12070:5238 GGAGATC +@HWI-ST575:165:C1P5BACXX:1:1101:12614:5234 TTACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:12934:5066 TGTATAC +@HWI-ST575:165:C1P5BACXX:1:1101:12889:5153 GGCAGCT +@HWI-ST575:165:C1P5BACXX:1:1101:13006:5008 CCCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:13701:5054 AATCAGC +@HWI-ST575:165:C1P5BACXX:1:1101:13818:5190 CCCATCG +@HWI-ST575:165:C1P5BACXX:1:1101:14721:5211 GCCGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:15180:5074 TCTATTG +@HWI-ST575:165:C1P5BACXX:1:1101:15733:5028 TGGGTAT +@HWI-ST575:165:C1P5BACXX:1:1101:16783:5148 TACACAC +@HWI-ST575:165:C1P5BACXX:1:1101:17093:5065 CGGTCGT +@HWI-ST575:165:C1P5BACXX:1:1101:18177:5052 TCATTAC +@HWI-ST575:165:C1P5BACXX:1:1101:18454:5064 CATATGC +@HWI-ST575:165:C1P5BACXX:1:1101:19514:5155 TTCTTAT +@HWI-ST575:165:C1P5BACXX:1:1101:21070:5172 GCATATC +@HWI-ST575:165:C1P5BACXX:1:1101:1152:5446 CATACAC +@HWI-ST575:165:C1P5BACXX:1:1101:2332:5476 CACCGTA +@HWI-ST575:165:C1P5BACXX:1:1101:3549:5422 TTTCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:3934:5255 TACATGG +@HWI-ST575:165:C1P5BACXX:1:1101:3779:5257 CGCGGTC +@HWI-ST575:165:C1P5BACXX:1:1101:3981:5396 TCCAGTC +@HWI-ST575:165:C1P5BACXX:1:1101:4352:5435 CTTACAC +@HWI-ST575:165:C1P5BACXX:1:1101:5757:5381 GTACATT +@HWI-ST575:165:C1P5BACXX:1:1101:7084:5290 GCATCCC +@HWI-ST575:165:C1P5BACXX:1:1101:7550:5435 CGCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8518:5454 GACGGAC +@HWI-ST575:165:C1P5BACXX:1:1101:8740:5499 CTTAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8810:5337 TGCACGC +@HWI-ST575:165:C1P5BACXX:1:1101:10317:5279 TGACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:11346:5362 ACAATTC +@HWI-ST575:165:C1P5BACXX:1:1101:12293:5318 AGCGACG +@HWI-ST575:165:C1P5BACXX:1:1101:12385:5426 CAAGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:12559:5359 GCGGGAC +@HWI-ST575:165:C1P5BACXX:1:1101:13619:5383 AGCGGTC +@HWI-ST575:165:C1P5BACXX:1:1101:14813:5367 TGCCCAT +@HWI-ST575:165:C1P5BACXX:1:1101:16958:5440 TCCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:17064:5269 CCTACAT +@HWI-ST575:165:C1P5BACXX:1:1101:18711:5374 TTGTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:19795:5388 GACTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:1536:5742 ACCCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:3186:5524 CTTCTCC +@HWI-ST575:165:C1P5BACXX:1:1101:3099:5544 CCAATCC +@HWI-ST575:165:C1P5BACXX:1:1101:4347:5721 ATCGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:5268:5661 TAACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:6568:5658 TGCAATC +@HWI-ST575:165:C1P5BACXX:1:1101:7344:5519 CACTAGC +@HWI-ST575:165:C1P5BACXX:1:1101:8216:5553 GGTACCC +@HWI-ST575:165:C1P5BACXX:1:1101:8183:5554 CTTAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8832:5696 AACGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:9211:5626 TACTATC +@HWI-ST575:165:C1P5BACXX:1:1101:9028:5675 TATTACT +@HWI-ST575:165:C1P5BACXX:1:1101:9789:5670 GCGTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:10507:5654 TCAAACC +@HWI-ST575:165:C1P5BACXX:1:1101:12148:5538 GACCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:12149:5686 ATCCAGC +@HWI-ST575:165:C1P5BACXX:1:1101:12546:5747 CACTTGG +@HWI-ST575:165:C1P5BACXX:1:1101:13615:5616 GCTATTC +@HWI-ST575:165:C1P5BACXX:1:1101:13537:5735 GCTTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:14584:5542 GCCGAAG +@HWI-ST575:165:C1P5BACXX:1:1101:15724:5746 TGTGGAT +@HWI-ST575:165:C1P5BACXX:1:1101:17227:5625 ACCAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:17881:5507 TAACCCT +@HWI-ST575:165:C1P5BACXX:1:1101:20112:5554 CTCTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:20322:5548 ACCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:21233:5679 CCCCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:1959:5920 GCAGTGT +@HWI-ST575:165:C1P5BACXX:1:1101:1924:5978 GCCTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:4153:5778 CGTGAGC +@HWI-ST575:165:C1P5BACXX:1:1101:4024:5788 CGTTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:4463:5945 CTGCGAC +@HWI-ST575:165:C1P5BACXX:1:1101:6188:5778 GCGGTCG +@HWI-ST575:165:C1P5BACXX:1:1101:6867:5946 CCACAAT +@HWI-ST575:165:C1P5BACXX:1:1101:7535:5814 TGCAACG +@HWI-ST575:165:C1P5BACXX:1:1101:7794:5903 ACAATGC +@HWI-ST575:165:C1P5BACXX:1:1101:9106:5832 CTCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:9392:5986 TTGACCC +@HWI-ST575:165:C1P5BACXX:1:1101:9549:5793 CTTATAT +@HWI-ST575:165:C1P5BACXX:1:1101:10541:5867 TCGCCCT +@HWI-ST575:165:C1P5BACXX:1:1101:11397:5818 AATCTAT +@HWI-ST575:165:C1P5BACXX:1:1101:13482:5805 TAAGGCT +@HWI-ST575:165:C1P5BACXX:1:1101:13731:5797 CACTAAG +@HWI-ST575:165:C1P5BACXX:1:1101:13983:5888 TGGCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:13915:5939 CGTGGTC +@HWI-ST575:165:C1P5BACXX:1:1101:14076:5802 CTCCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14165:5883 ATGCTTC +@HWI-ST575:165:C1P5BACXX:1:1101:14716:5991 CGCCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:15153:5829 CCCACCC +@HWI-ST575:165:C1P5BACXX:1:1101:15618:5813 GGGAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:16278:5962 GCCGACC +@HWI-ST575:165:C1P5BACXX:1:1101:17404:5768 AGTCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:18508:5770 ATACGGT +@HWI-ST575:165:C1P5BACXX:1:1101:18654:5893 GTTGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:20005:5823 TCTATAC +@HWI-ST575:165:C1P5BACXX:1:1101:20461:5768 TCTTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:1693:6048 AACCCTT +@HWI-ST575:165:C1P5BACXX:1:1101:3291:6004 CCCACCC +@HWI-ST575:165:C1P5BACXX:1:1101:3937:6198 CCTTCTC +@HWI-ST575:165:C1P5BACXX:1:1101:4273:6038 CACGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:4445:6115 CTGCAGC +@HWI-ST575:165:C1P5BACXX:1:1101:4344:6153 AGTGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:5212:6077 GCTCGTC +@HWI-ST575:165:C1P5BACXX:1:1101:5854:6193 TTGACGA +@HWI-ST575:165:C1P5BACXX:1:1101:6093:6246 AAGGGCT +@HWI-ST575:165:C1P5BACXX:1:1101:8105:6158 GTCTAGC +@HWI-ST575:165:C1P5BACXX:1:1101:8253:6223 GATAGCC +@HWI-ST575:165:C1P5BACXX:1:1101:8552:6031 GCTAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:8575:6101 ACTACTA +@HWI-ST575:165:C1P5BACXX:1:1101:9569:6015 CAACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:10905:6024 GCAGCCT +@HWI-ST575:165:C1P5BACXX:1:1101:11477:6010 CCGTTGT +@HWI-ST575:165:C1P5BACXX:1:1101:12389:6154 CCACCTC +@HWI-ST575:165:C1P5BACXX:1:1101:12820:6019 ACACCCC +@HWI-ST575:165:C1P5BACXX:1:1101:13503:6047 CCGCTCA +@HWI-ST575:165:C1P5BACXX:1:1101:14071:6041 TTATGAC +@HWI-ST575:165:C1P5BACXX:1:1101:14685:6102 ACTTACC +@HWI-ST575:165:C1P5BACXX:1:1101:14930:6229 GCCCATC +@HWI-ST575:165:C1P5BACXX:1:1101:15280:6053 CCCGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:15344:6191 CAAATCC +@HWI-ST575:165:C1P5BACXX:1:1101:16410:6086 CGCTGGT +@HWI-ST575:165:C1P5BACXX:1:1101:16374:6145 CCTGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:16749:6158 CGGCTAA +@HWI-ST575:165:C1P5BACXX:1:1101:17553:6192 CACCTAC +@HWI-ST575:165:C1P5BACXX:1:1101:17938:6081 TAAGTGC +@HWI-ST575:165:C1P5BACXX:1:1101:19315:6150 TTACCTC +@HWI-ST575:165:C1P5BACXX:1:1101:19532:6018 CACCCTC +@HWI-ST575:165:C1P5BACXX:1:1101:21261:6248 ATACATC +@HWI-ST575:165:C1P5BACXX:1:1101:1089:6262 AACATGC +@HWI-ST575:165:C1P5BACXX:1:1101:1439:6319 GCGGTTC +@HWI-ST575:165:C1P5BACXX:1:1101:1829:6451 CGGATAT +@HWI-ST575:165:C1P5BACXX:1:1101:2484:6444 GAATGAC +@HWI-ST575:165:C1P5BACXX:1:1101:3350:6364 CATTGCG +@HWI-ST575:165:C1P5BACXX:1:1101:3699:6336 CAGGCTT +@HWI-ST575:165:C1P5BACXX:1:1101:3874:6280 AACCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:4404:6415 CCAAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:5416:6451 CAATGTT +@HWI-ST575:165:C1P5BACXX:1:1101:7066:6455 CCTAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:7488:6251 GCGAGCC +@HWI-ST575:165:C1P5BACXX:1:1101:7486:6329 TAACCAT +@HWI-ST575:165:C1P5BACXX:1:1101:7643:6450 AAAGCCT +@HWI-ST575:165:C1P5BACXX:1:1101:8454:6487 CACAGTT +@HWI-ST575:165:C1P5BACXX:1:1101:9137:6329 TGACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:9587:6277 TATTACC +@HWI-ST575:165:C1P5BACXX:1:1101:9536:6348 CAATTCC +@HWI-ST575:165:C1P5BACXX:1:1101:10054:6289 ACCTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:10173:6294 CAAAGAT +@HWI-ST575:165:C1P5BACXX:1:1101:10764:6333 ATTCGCC +@HWI-ST575:165:C1P5BACXX:1:1101:11047:6477 TATACGT +@HWI-ST575:165:C1P5BACXX:1:1101:11411:6372 AATCCGC +@HWI-ST575:165:C1P5BACXX:1:1101:11665:6448 CCCTGAC +@HWI-ST575:165:C1P5BACXX:1:1101:12774:6457 TATATAC +@HWI-ST575:165:C1P5BACXX:1:1101:13106:6369 CAGGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:16704:6321 CCCTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:16660:6380 CACTCTT +@HWI-ST575:165:C1P5BACXX:1:1101:17964:6393 TTCGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:18173:6282 CTTTAAC +@HWI-ST575:165:C1P5BACXX:1:1101:18209:6492 TACACAC +@HWI-ST575:165:C1P5BACXX:1:1101:18546:6430 TACTTCT +@HWI-ST575:165:C1P5BACXX:1:1101:19023:6315 CGATTCC +@HWI-ST575:165:C1P5BACXX:1:1101:19100:6466 GACTCCT +@HWI-ST575:165:C1P5BACXX:1:1101:20025:6400 CTCATAT +@HWI-ST575:165:C1P5BACXX:1:1101:20043:6415 TCACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:1982:6500 TCGACAC +@HWI-ST575:165:C1P5BACXX:1:1101:1934:6651 TGACACG +@HWI-ST575:165:C1P5BACXX:1:1101:3031:6587 TCCGATC +@HWI-ST575:165:C1P5BACXX:1:1101:3659:6632 TAGGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:4271:6639 GCGTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:5521:6516 ACAGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:6888:6616 GCACACT +@HWI-ST575:165:C1P5BACXX:1:1101:6970:6733 CCTAGCT +@HWI-ST575:165:C1P5BACXX:1:1101:9786:6627 ATCACAC +@HWI-ST575:165:C1P5BACXX:1:1101:10466:6616 CGCCGAT +@HWI-ST575:165:C1P5BACXX:1:1101:12094:6626 GAGGACG +@HWI-ST575:165:C1P5BACXX:1:1101:13236:6588 GCTCGGG +@HWI-ST575:165:C1P5BACXX:1:1101:13123:6721 GGTAATC +@HWI-ST575:165:C1P5BACXX:1:1101:13878:6710 CGACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14181:6588 GGATCAG +@HWI-ST575:165:C1P5BACXX:1:1101:14627:6506 TACGACC +@HWI-ST575:165:C1P5BACXX:1:1101:15075:6709 TTTCTTC +@HWI-ST575:165:C1P5BACXX:1:1101:16741:6696 GTTACAC +@HWI-ST575:165:C1P5BACXX:1:1101:16765:6674 TCATAAC +@HWI-ST575:165:C1P5BACXX:1:1101:19358:6651 GTTGAAC +@HWI-ST575:165:C1P5BACXX:1:1101:19561:6627 TTACAAC +@HWI-ST575:165:C1P5BACXX:1:1101:20819:6735 TTTGCAT +@HWI-ST575:165:C1P5BACXX:1:1101:1564:6811 TACGCAC +@HWI-ST575:165:C1P5BACXX:1:1101:1927:6913 GCTAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:2572:6857 CACCGTC +@HWI-ST575:165:C1P5BACXX:1:1101:3215:6869 TGCACAC +@HWI-ST575:165:C1P5BACXX:1:1101:3121:6916 GACATAT +@HWI-ST575:165:C1P5BACXX:1:1101:5462:6854 CACAAAC +@HWI-ST575:165:C1P5BACXX:1:1101:6911:6752 AACAACG +@HWI-ST575:165:C1P5BACXX:1:1101:8402:6992 TCCCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:10199:6779 TTTGAGC +@HWI-ST575:165:C1P5BACXX:1:1101:11749:6891 GATTTAC +@HWI-ST575:165:C1P5BACXX:1:1101:12769:6834 GCTGAAT +@HWI-ST575:165:C1P5BACXX:1:1101:13711:6990 GCGCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:14111:6893 GTTATGC +@HWI-ST575:165:C1P5BACXX:1:1101:14440:6994 CTGAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:15694:6869 CCGCATC +@HWI-ST575:165:C1P5BACXX:1:1101:16093:6801 ACCAGCC +@HWI-ST575:165:C1P5BACXX:1:1101:16736:6834 GACGTAC +@HWI-ST575:165:C1P5BACXX:1:1101:16688:6982 CGAAGAC +@HWI-ST575:165:C1P5BACXX:1:1101:17036:6768 ACCGAGC +@HWI-ST575:165:C1P5BACXX:1:1101:17542:6830 TTCCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:18093:6840 TCTATAC +@HWI-ST575:165:C1P5BACXX:1:1101:18333:6967 AACCAAC +@HWI-ST575:165:C1P5BACXX:1:1101:19230:6988 GCGCCAC +@HWI-ST575:165:C1P5BACXX:1:1101:20486:6800 CAGTCAC +@HWI-ST575:165:C1P5BACXX:1:1101:20297:6938 AAATCCC +@HWI-ST575:165:C1P5BACXX:1:1101:20449:6990 ACACAAT +@HWI-ST575:165:C1P5BACXX:1:1101:1103:7035 GATGTGC +@HWI-ST575:165:C1P5BACXX:1:1101:1500:7064 GAGGCTC +@HWI-ST575:165:C1P5BACXX:1:1101:1577:7179 AGATATC +@HWI-ST575:165:C1P5BACXX:1:1101:2297:7148 GTAAACG diff -r fb76303acf4f -r 431aebd93843 test-data/k2n.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/k2n.txt Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,58 @@ +1 2 3 4 5 +6 7 8 9 10 +11 12 13 14 15 +16 17 18 19 20 +21 22 23 24 25 +26 27 28 29 30 +31 32 33 34 35 +36 37 38 39 40 +41 42 43 44 45 +46 47 48 49 50 +51 52 53 54 55 +56 57 58 59 60 +61 62 63 64 65 +66 67 68 69 70 +71 72 73 74 75 +76 77 78 79 80 +81 82 83 84 85 +86 87 88 90 91 +92 93 94 95 96 +97 98 99 100 101 +102 103 104 105 106 +107 108 109 110 111 +112 113 114 115 116 +117 118 119 120 121 +122 123 124 125 126 +127 128 129 130 131 +132 133 134 135 136 +137 138 139 140 141 +142 143 144 145 146 +147 148 149 150 151 +152 153 155 156 157 +158 159 160 161 162 +163 164 165 166 167 +168 169 170 171 172 +173 174 175 176 177 +178 179 180 181 182 +183 184 185 186 187 +188 189 190 191 192 +193 194 195 196 197 +198 200 201 202 203 +204 205 206 207 208 +209 210 211 212 213 +214 215 216 217 218 +219 220 221 222 223 +224 225 226 227 228 +229 230 231 232 233 +234 235 237 238 239 +240 241 242 243 244 +245 246 247 248 249 +250 251 252 253 254 +255 256 257 258 259 +260 261 262 263 264 +265 266 267 269 270 +271 272 273 274 275 +276 277 278 279 280 +281 282 283 284 285 +286 287 288 289 290 +291 292 293 294 diff -r fb76303acf4f -r 431aebd93843 test-data/read_counts.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/read_counts.txt Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,108 @@ +RNase_P 110 187 2 +RNase_P 111 187 4 +RNase_P 112 187 2 +RNase_P 115 187 1 +RNase_P 117 187 5 +RNase_P 117 188 1 +RNase_P 119 186 1 +RNase_P 134 188 1 +RNase_P 137 187 1 +RNase_P 138 187 1 +RNase_P 152 187 1 +RNase_P 10 187 1 +RNase_P 11 187 1 +RNase_P 15 187 2 +RNase_P 30 187 2 +RNase_P 31 186 1 +RNase_P 31 188 1 +RNase_P 18 186 1 +RNase_P 18 187 1 +RNase_P 19 187 1 +RNase_P 32 187 1 +RNase_P 33 187 1 +RNase_P 50 187 2 +RNase_P 51 187 1 +RNase_P 38 187 1 +RNase_P 39 187 1 +RNase_P 55 187 3 +RNase_P 56 187 1 +RNase_P 2 187 2 +RNase_P 57 187 2 +RNase_P 71 187 2 +RNase_P 3 185 1 +RNase_P 3 186 9 +RNase_P 3 187 96 +RNase_P 3 188 1 +RNase_P 58 187 3 +RNase_P 59 187 1 +RNase_P 4 185 1 +RNase_P 4 186 32 +RNase_P 4 187 307 +RNase_P 73 187 1 +RNase_P 5 187 14 +RNase_P 6 186 2 +RNase_P 6 187 10 +RNase_P 7 187 4 +RNase_P 8 187 4 +RNase_P 90 187 2 +RNase_P 77 187 4 +RNase_P 9 187 3 +RNase_P 78 187 2 +RNase_P 92 187 2 +RNase_P 93 187 1 +RNase_P 94 187 2 +RNase_P 100 187 1 +RNase_P 97 186 1 +RNase_P 97 187 4 +RNase_P 98 187 1 +RNase_P 101 187 2 +RNase_P 102 187 1 +RNase_P 99 187 5 +RNase_P 103 187 1 +RNase_P 104 187 1 +RNase_P 105 187 1 +RNase_P 106 187 2 +RNase_P 107 186 1 +RNase_P 107 187 3 +RNase_P 121 187 2 +RNase_P 109 187 2 +RNase_P 125 187 1 +RNase_P 126 187 2 +RNase_P 128 187 3 +RNase_P 142 187 1 +RNase_P 129 187 1 +RNase_P 145 187 1 +RNase_P 147 187 1 +RNase_P 148 187 1 +RNase_P 163 187 1 +RNase_P 169 187 1 +RNase_P 20 187 1 +RNase_P 21 186 2 +RNase_P 21 187 7 +RNase_P 22 187 2 +RNase_P 23 185 1 +RNase_P 23 187 4 +RNase_P 24 187 1 +RNase_P 25 187 1 +RNase_P 40 186 1 +RNase_P 40 187 7 +RNase_P 27 186 1 +RNase_P 27 187 1 +RNase_P 41 187 1 +RNase_P 42 187 1 +RNase_P 43 187 1 +RNase_P 44 186 1 +RNase_P 44 187 1 +RNase_P 45 187 2 +RNase_P 48 186 1 +RNase_P 63 187 1 +RNase_P 64 187 1 +RNase_P 65 187 1 +RNase_P 68 187 1 +RNase_P 81 187 2 +RNase_P 82 187 2 +RNase_P 83 187 1 +RNase_P 85 187 2 +RNase_P 86 187 1 +RNase_P 89 147 1 +RNase_P 89 187 6 diff -r fb76303acf4f -r 431aebd93843 test-data/reads1.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reads1.fastq Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,2684 @@ +@HWI-ST575:165:C1P5BACXX:1:1101:1666:1132 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +DHHHFGGIJEGGGHGIIJDHDFDFHAGCB=FCGGEGHHGGIGEEBBDCBBBBCC +@HWI-ST575:165:C1P5BACXX:1:1101:3207:1238 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FADFFAEGGIEGIEBHG@GEA@BBGHEF>F8?78B@FCGGICEGECCABA?BA@=?C? +@HWI-ST575:165:C1P5BACXX:1:1101:3405:1152 +TGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCT ++ +DFDFD2@CEB:<3)?EF;C:91?DGBF??BBF<0909B?DDB26= +@HWI-ST575:165:C1P5BACXX:1:1101:3512:1179 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGGHGHJJJJJIJJIIJIJIIHJHJJJGIIJJJJJJJJJGJHIIIIGEHGFFDDBDDDDDB?ACCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4473:1182 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FFHFHF@GGIIIDII:FFBDFHGGEHGIIBGGGGGIGICGIG;DGE>CHFFCA +@HWI-ST575:165:C1P5BACXX:1:1101:5162:1092 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGHIIJJJJJJFHIIJHIJJIJJJJJJJJJIGHJJJIJIIIIIJHHGFDDDBBDDDDDDDDDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:6810:1177 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHFHHJJJJJJJJJIJIIJJGIJIJJJIIJJJJJJJJJJJJIJJJJIGHGFFDD?BD +@HWI-ST575:165:C1P5BACXX:1:1101:8268:1157 +TCGGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCCTCGGCGGATCCAA ++ +FGHHHHJJJJJIJJIJIJJJIIIJIBFHIJJIIJJJJJJIGDFFEEEDDDDDDDDDDDDDDDDDDDDDDD@@>C>C +@HWI-ST575:165:C1P5BACXX:1:1101:8740:1185 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGFHHHJJJJIJJJJJJIJIGIJIJJJJIJJGIJJJJJIIIJJJJIJHHHFDDDDDD;AB@BBDCCBD +@HWI-ST575:165:C1P5BACXX:1:1101:12414:1099 +GATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGA ++ +FFHHHHJJJJJJIJJJJJJIGIJGFGGHJJJJGEIIJJJJIIHHHIIIHHHHHHFDFFFD +@HWI-ST575:165:C1P5BACXX:1:1101:14053:1112 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHFHJJJJJJHHHIJIJJIJJJJJJJGDIIJJJJJIJJJGGJIIIJHHFFDDBBD9 +@HWI-ST575:165:C1P5BACXX:1:1101:14219:1152 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJIHIHGIFGIIJJJJJJIIJJJJJJIJIIJFIIJIJJHHFFDDB??C9A +@HWI-ST575:165:C1P5BACXX:1:1101:15012:1211 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FDFHHHIJJJJIJJGJJIIJJJJIJJJI@EGIJJJJJJHGJJIJHIFHHFFDDDBACA3> +@HWI-ST575:165:C1P5BACXX:1:1101:19911:1138 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHDHHDGIHIIIIIIDGGHIICGFHIIIGIIIIIIIIIIIIHHIIHHIFFFFCC?=CC; +@HWI-ST575:165:C1P5BACXX:1:1101:1311:1500 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHHIJJJIJHHBGGHGIICHGFHIJJIHHIIIEIJHIIJIHGHFFHFFDDDBB?ABBDD@CDCBBDCDDDBB1=;;5 +@HWI-ST575:165:C1P5BACXX:1:1101:5495:1254 +ACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAA ++ +FHHHGHJJJJICFGGIJHJJJFFFHIJGHJJJGIIIIIIJIFFFDDDDDDDDDDDDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:5684:1314 +AGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC ++ +FHHHGFHHGHIIJIJJIIJJJJJFCHHIHJIJIJCHIJJJJJIGGIGHIJJIGIHHHHHFFFDD +@HWI-ST575:165:C1P5BACXX:1:1101:6002:1450 +GCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +FHHHHHJHIIJIJJIIIIJJIGHIIJJJJJBGHGJJFGDEGEFHGIBEHHCEFFFCADDBDD@BBBB +@HWI-ST575:165:C1P5BACXX:1:1101:6163:1455 +TTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHHHGIJIJIIIIJICHCHGIHIJIEGHHIIJGHGHFHIIJJIF4ADDDBDDDDDDDDDDB@DCCCCDCDB +@HWI-ST575:165:C1P5BACXX:1:1101:6370:1342 +GAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +DBFDHFGIB??EFH?FGDHHB?D@B@DAHGIIIGGII9@7CAFFCAHADCCH?CACD +@HWI-ST575:165:C1P5BACXX:1:1101:7500:1303 +TTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGA ++ +FHHHHGJHHJJJJJJHJIJJDGIGIIJIIJJIIJJJIJHHIJIJJIJJGIIGIIHDEHFFFFDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8186:1308 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGAAGGAAA ++ +FDACFFHIEIGIIE8CHE6BDFGEDCBBD@BCFHHHIDHIIIHBCH>=CD>9?779?DB?39< +@HWI-ST575:165:C1P5BACXX:1:1101:10737:1349 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAAGCCTACGT ++ +FHDFHHIJJJJJIIIJJGGIGIIGIJJIIJIJJJIJJJHHHFEFFFFECDDDDD95? +@HWI-ST575:165:C1P5BACXX:1:1101:11993:1308 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHADGIIIGIHIJJJEGGGGIGIIIJIJJJJJGGGGIIIIIFHHDHFDADDB?ACDBD +@HWI-ST575:165:C1P5BACXX:1:1101:12260:1420 +TGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJGIJJIIJJJIIIJJJIHGJJJEIHGGHJJJJIIGFFFDEED;;=9?CCCDB +@HWI-ST575:165:C1P5BACXX:1:1101:13041:1365 +GGGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FAHFFHGFGIJJJIJGHIIIGIJIIAHIICFGGJG@@DHHIEHFEHEDFCDDD@BB19>CBD +@HWI-ST575:165:C1P5BACXX:1:1101:13915:1290 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGFHHJJJJIJIJJEHIHIJIJEIJIJJGGIIJIIIIJJJIEIIJJIIJHHFDDBBC??@BDBDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13944:1432 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHGHJJJIFIIJIJJHJIIJIIIIIJJJJJJJIJJJJJJJGJIJHHHFFDDDBBC(9 +@HWI-ST575:165:C1P5BACXX:1:1101:14362:1371 +ACGACGCTCTTCCGATCTTCACCACAGACG ++ +DDHDHHIBE@EFHBC;??D90??DBBGHHI +@HWI-ST575:165:C1P5BACXX:1:1101:14865:1283 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+GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFFHHIGIJIJIIHJJGIGIADFGGGHIJIIJJJHIJIJIJGGIJGJJHGFDDC?CC9 +@HWI-ST575:165:C1P5BACXX:1:1101:20338:1414 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAG ++ +DDCDDCAEEEEIII1)::9D>BDBDICDDI;DDDBCDEBDCEIIIDCDDDD8? +@HWI-ST575:165:C1P5BACXX:1:1101:1782:1682 +GGTCGTGCCTAGCGAAGTCA ++ +DDD1C0C+22?A@BABDC?BB +@HWI-ST575:165:C1P5BACXX:1:1101:2796:1586 +AGGTCGTGCCTAGCAAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGA ++ +FHHHHHIJIJJJJJIJIIHIJIJJIJJJIIIJJJJIJJIJFJIGIJJGJGHJJHFFFFCDDB;?ACCBBDDDDA@< +@HWI-ST575:165:C1P5BACXX:1:1101:3075:1523 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHAHFHGGHIIJJJIJJIHIIGICHHIG@HIJJHHIJIJJIHIHIIGHEFEDDB?ACC +@HWI-ST575:165:C1P5BACXX:1:1101:4251:1515 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFHHFIJJJJIJJJIJIHIIJJIJJJJJJJIJJGIJJJJIIGIJIIHHHFFDD=BD?B +@HWI-ST575:165:C1P5BACXX:1:1101:4481:1718 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHHJJJJJJJJJIJHIJIJJIJJJJHJJJIIJJJJJIGIIJJJJHFFFADDDDDDDDDDDDDDB@ADDCBD82 +@HWI-ST575:165:C1P5BACXX:1:1101:4978:1615 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJJJJIJIIIIGIJJJHIJJJJIJJHHGGHGDFFEECDDDDD?BCCBDB?BDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6935:1687 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FGDDHHHIJIJIJHGGGIFE5=AB +@HWI-ST575:165:C1P5BACXX:1:1101:7646:1624 +TTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACC ++ +FHHHHHJJJJJJJHHJJJJJJIJJJJJJJJJJJJJJJIJJJJJIHIIJJJIIGIJJIIJHFFFFEEDDDBD?;2 +@HWI-ST575:165:C1P5BACXX:1:1101:8080:1642 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHFJJJJJIJJJIIIJJJJJGJJJJIJJJJJJJJJIJJJIJJIJJGHFBAB@DCD +@HWI-ST575:165:C1P5BACXX:1:1101:8452:1710 +AAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ 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+CGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGCAAAAAGCCTAC ++ +FFHHHHJIJIEIIJIHIIJJJJJIIIIIJIHIIJIJIIIGHIIJIJBEHFF';AE@B?ABDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11536:1555 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGAGGGCAGGAAAAAAGCCTACGT ++ +FHHHGHJJJJIIJJJJJIJIIIJGIFIJJIJJJJIIJJIGIJIGHIHHHFFDDCDBCD@BB?ACACBB +@HWI-ST575:165:C1P5BACXX:1:1101:11540:1624 +GGGGCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHHHHFFFFFDDDDDDEDDDCCDDDDBDDBCDDEDDDDDDDDDDDDDDDDBBDCCB +@HWI-ST575:165:C1P5BACXX:1:1101:12292:1617 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGHIIIJJIJJJJIIHGIGIEIJIIEGIJJJJEIJJJIJIIJIFFEFFDDDBDDDBBB98>>CBA +@HWI-ST575:165:C1P5BACXX:1:1101:13423:1569 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJHIHIIIIIIIIIIJJJJJJJJJIJJJJIIJGJIIIJHHFFDC???CBDB@DDCDDB +@HWI-ST575:165:C1P5BACXX:1:1101:13576:1612 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGHGHIJIJJJJJJJIIJIIJJGIJIJJIJJJGIJJJJIIJJIHHFEEDDDDBBBDCABB8?CC?>< +@HWI-ST575:165:C1P5BACXX:1:1101:14491:1526 +CGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHGFHHEHIIJIJJJ@GHAFIIJDHIGIIHHIGIIGGIIJJJJDIIJGHHFFDBB?A>? +@HWI-ST575:165:C1P5BACXX:1:1101:15109:1589 +CCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHGHDFGIHIGHIGIJGIJJGJBGGGGHHHFFDB=@DCCDDA@FEHIDHIJJFHGHGHF6?B39>=5>(5 +@HWI-ST575:165:C1P5BACXX:1:1101:17059:1656 +GGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTTAGAGTGGAACTCGGTAAACCCCTC ++ +FHHHHHJHGIJIIJIIJJJIJIJIJEIGHJJJIIJJJJJJJJEIICHEHIHIGGAHHHHAAEEDFFDCCEAC?BDD +@HWI-ST575:165:C1P5BACXX:1:1101:17823:1711 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHFIJJJIJIIFGI=GIHJJJIGIJJJJIIGHIJJJJGJJGIJJHHHFDDDDDC59@@?BDDDDBBCBDBD@? +@HWI-ST575:165:C1P5BACXX:1:1101:18750:1719 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHDGEHIEIBGBEEDHGGGIHGGEHIGIGGGIIJIHIEGECG@EEEEF?>C(5>,58 +@HWI-ST575:165:C1P5BACXX:1:1101:19397:1535 +TGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJIJIJBEH?FHGADDGHJIJIJJIFHIJJJIIJI?HIIJIIJFFFCAEC; +@HWI-ST575:165:C1P5BACXX:1:1101:19270:1553 +TCTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTT ++ +DHHDHHGHIIJJIEHHGIJJIGGCGGFBGHJGHIJIJGGIGHHHC>BDEED6@CACDC +@HWI-ST575:165:C1P5BACXX:1:1101:20160:1653 +GCTCTTCCGATCTGGACTCTCGG ++ +DHHHFHHIGIJJIHGGGGGIIE7 +@HWI-ST575:165:C1P5BACXX:1:1101:20348:1677 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHCCFGIIGIIJEGGHIGGIGIJJEHJIJIIGIIIGIJJJIJJIJIGGIJHFFDBC?(==@AGECF??BDFBE39BDDABABBF>FFFCFCCEEFCEEFDCCB<;?; +@HWI-ST575:165:C1P5BACXX:1:1101:2146:1765 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHFHFHIJJJJJJJIJIIIGIGIGHIJJJJJJIIJIJJIJIJJFIJIEEHFDDD@BCDDDBBDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:2817:1860 +TTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATC ++ 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+ACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGA ++ +FHHHHHJIJJJJJJIJIJJJJGGHIJGIJJFGIIJJJIJJJJIJIJIHIJIIJJJJJDH +@HWI-ST575:165:C1P5BACXX:1:1101:11078:1760 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHFHHJIJIIJJJJGJJGIJIJJJIJJJIJJJJHIIJJJJJIJJHHHHFFDDDDBB9AABBA?CDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12091:1910 +TCACAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHHHJIJJJJIJJJJIJJJIJJJJJJJJJIJIJJJIJJJIHHHFFFFDEEEDCDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13182:1885 +GGTCGTGCCTAGCGAAGTCATAAGCTAGTGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHGJJIJJJJJJJJJJIIIJIJJJJGGIIJJJJJJJJIJIJIIJJJHHFFD@ACCB +@HWI-ST575:165:C1P5BACXX:1:1101:13475:1836 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJGIJJJJJJJJIIJGIIJJJIJJJJJJJJJJIJIIIJIHHHFDDDBDDDAB?9?CCCDB +@HWI-ST575:165:C1P5BACXX:1:1101:14275:1813 +AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHHIJJJJJJJIJJGIIIIDGIJIGHGIIJGIIIJJIIIIJJJIJIHHFDDAB;?;?BBBCDABA +@HWI-ST575:165:C1P5BACXX:1:1101:14914:1754 +TCAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +FHHHHHJJJIIJJJJJIJJJJIJIJJIHJJIJJGGIIGIIJIJJIJJIIIJHGGFFDDDDDBDD +@HWI-ST575:165:C1P5BACXX:1:1101:15826:1814 +TCTGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHGHDHGHHIIIIGIIIJGHGIEIHGGIF>AECBB?CDDCDD@; +@HWI-ST575:165:C1P5BACXX:1:1101:16316:1995 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +DHHFFHGFFGGHHIEGGFHGIIIIJJ>DGHGIIGIIIIGIHIGHIIIDDGHHHHHFFC?B8(BAACDDBD +@HWI-ST575:165:C1P5BACXX:1:1101:1102:2244 +ACGACGCTCTTCCGATCTTCCTGCTAGACGTGG ++ +D=DF8D:CEFF@GG6FGD>@CHGED>;AADBAB';5? +@HWI-ST575:165:C1P5BACXX:1:1101:3289:2146 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGC ++ +?FDFDHIIGHGIFGGGGGHGIEGHFHIGGGHGHGGC?FHI@G>FGEGGEAE +@HWI-ST575:165:C1P5BACXX:1:1101:4895:2080 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGHGHHJJJIJJFIJHJJIJJIIIIJJGHIGIJJGIJJJJGJEIJJIIJHHF8A@?B>C +@HWI-ST575:165:C1P5BACXX:1:1101:4932:2108 +CGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +DDDDFFGEHGA=GHII@6@0DFCH>@AAAEB8@BB(>3:C@@- +@HWI-ST575:165:C1P5BACXX:1:1101:5806:2073 +ACGACGCTCTTCCGATCTGTACCATCAGACGG ++ +DHHGHAHBHIGIGDABACGF@DFD@;?>FHA: +@HWI-ST575:165:C1P5BACXX:1:1101:6214:2065 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHDHFDHIJJJJGIHGGIHGHJGEHIGIBGGIJDDHIIJJIJJJIIJGFEEDAB=ACD@ +@HWI-ST575:165:C1P5BACXX:1:1101:6143:2137 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGG ++ +DAF@DFCGDHIBG?DGIGDFEHEF>CDGD?CCFB;FA@F>GG9CHHC=?B> +@HWI-ST575:165:C1P5BACXX:1:1101:6241:2199 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DHHHHHIJJJJIHIHJIGIIJJJJJIJJIJIIJGIIEIICGHCIIJJHHGDCB8=ACD +@HWI-ST575:165:C1P5BACXX:1:1101:7894:2030 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHIIJJJJJJIJJIJEGIJIJJJJIJJIJJJJJJJIIIIJIJHHHFFCDD?ACDDDBDDDDCBB 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+GTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHAFHGJJIIFFHIJIJGIIGHGBGEHIHFCBBDABD>?@@87A99>CC?< +@HWI-ST575:165:C1P5BACXX:1:1101:12469:2156 +GGTCTTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAGCCTACGT ++ +FHHHHGJJJJJJIGIIJIJJIJJIJJJJJJJJJJIJJJHIIIIIJJIJIHHFF@?BCCB?=ACD<=? +@HWI-ST575:165:C1P5BACXX:1:1101:13307:2211 +TTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAG ++ +FHHHHHIJJHJJJJJJJIJJJJJJDBFHIJJJJIJJJIHGGFFFFFCBACECEDEDACACC +@HWI-ST575:165:C1P5BACXX:1:1101:13967:2112 +TGTGACGAAGTCTCACTAGAAATGGTGAGAGGGGAACGCGGTAAACCCCTC ++ +DA=DFA;<@B<:AA:A@HE;:BD +@HWI-ST575:165:C1P5BACXX:1:1101:14357:2064 +TGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAT ++ +FHHHHGIJIJJJJJJIIIIJIJJ9EFHIJGHHGGIJJJJIGIJGGGIJHGFFFD>BDDDCDDDDDDDDD@BDA@C4 +@HWI-ST575:165:C1P5BACXX:1:1101:14498:2106 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJJJJJJHIIIJIIJJJJJJJJJJIJJJJJGHIJJJJIHHFFDDDBD3= 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+GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +FHHHGHEGIIJIGJJBHBHIIJJJGGIGIJFGIHGEFHIGIIFGIJHHHFFC?BB? +@HWI-ST575:165:C1P5BACXX:1:1101:16935:2365 +AAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGGGC ++ +FFHHGHJJIIJJIIJJJJDEGHJIGEHGCHIGJJJJJJGHCHHIJIJFEDDDBDDDDDDDBDD@DBDDCCCCB959 +@HWI-ST575:165:C1P5BACXX:1:1101:17289:2412 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHHIJIIDIJJEHIDGEGIGGIJJJJJJJJHJIJJFIIJIIHHHHFFD>BB???99 +@HWI-ST575:165:C1P5BACXX:1:1101:18384:2478 +GCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCT ++ +FGHGHHFGHIJIJIJJJJJJIJDGIJJIJIJIJJJJJJJJIJJFHCHFDDCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19068:2414 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHFHHIIIBHHIII:EHFGIEIII*?FHDHHIIFAHCHIIGIIIIIGCHHFFCC15?;?@B<CD +@HWI-ST575:165:C1P5BACXX:1:1101:20888:2259 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTA ++ +FHH@FFHIIIIGIBF6?DDGI9FIFB=?C 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+TAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +FHHHHHJJJJIJJJJIGJJJIFIJJJIJJJJJJGFGHJJIHHHHDDDFC@CEEACCCC>ACCDDDD@CCCD +@HWI-ST575:165:C1P5BACXX:1:1101:13467:2781 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGGHGDHIGIIICHGBHIIJIIIEGJIJFHIJJIIJIHHIHIFIGGIHBHFDD@7ACCDDB2A +@HWI-ST575:165:C1P5BACXX:1:1101:13716:2837 +GATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAA ++ +FGHHHHJJJIIJIIJJIIJIIJJJGIJJJJJJHGJJJJIJIFIHHGHGGGIJIIGHICHGGACC?E +@HWI-ST575:165:C1P5BACXX:1:1101:14400:2843 +AGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAG ++ +FHHHHHJJJJJJIIIJIIJJIJGGJJJJJJJJJIGGGGIJHHHHB@DDFEEEEEECC@C +@HWI-ST575:165:C1P5BACXX:1:1101:14816:2802 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHHHJJJJJJIJJJIIJJJIJHJJJJJJJJJJIJJIIJJJJGIJHHHHFFFFEDC;=;@CDD +@HWI-ST575:165:C1P5BACXX:1:1101:15055:2926 +GTCGTGCCTAGCGAAGTTATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ 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+TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATAT ++ +D?@8CFGFEGBFHEHFHIIIHFHGICEEHIIFHHHIIGHHIIIHGHEFFFCC??A?A8?BBC>CBCB9?C15885+ +@HWI-ST575:165:C1P5BACXX:1:1101:14598:3012 +CCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTC ++ +FFHFFHFEEHDIGG;CCCAFHBFC9CBGCAHG@E6@@1BBCEG;=E;???@CC +@HWI-ST575:165:C1P5BACXX:1:1101:16490:3172 +GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJIJJJJGIJIJIGIJIIIIJJJJJJJIJJJJJGIJJHHAHIHHFDDADDC==@9?CDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:16810:3009 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHHHHHJIJJJJJIHIJCGIIJJIJIJJJJJJJIJJJJJJGGIIIHHHHFDDDD@DD<2=;<<>A +@HWI-ST575:165:C1P5BACXX:1:1101:11999:3288 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCTGGAAAA ++ +FHHFHHHIIIGDIIJEIGDEIEGIGGIGIGJJFG=FHIJFGHIJJJEEHHFD/;@=C>A +@HWI-ST575:165:C1P5BACXX:1:1101:12147:3289 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ 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+AGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +FHHHHGIJJIJIIIIJJIJJJJIEGHJJIJJJIJIJJIJJIIHIIJIJIIJJJHHHHHF +@HWI-ST575:165:C1P5BACXX:1:1101:16628:3381 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJIJJJJJJEGIGIGIIJIJJIJJJJIJIJJJJFIIIIJJJHGHFFDDDBBA<9=69ACDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:1080:3578 +GGTCNTGCCTAGCGAAGTCANAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGANAAAANCCTACGTC ++ +FGFH#2>EHIHIIHGHHHII#0?DHGIIIIIIGHIBHGCG>EIIJF@ADD=ABDB#,5??#,5<AA +@HWI-ST575:165:C1P5BACXX:1:1101:1525:3749 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTGGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +DHFFFHHHJHGGHIHGHHIIHIEEIJIIGHHCHHIEEE)?ADDDDEECDB?B=??ACCBBBDDDDC@B +@HWI-ST575:165:C1P5BACXX:1:1101:2431:3630 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHGCFHHIIJJIIIJIGH?FHGIJGIGH>FFAD@CCCEEEEHFFBFAC@ADDD?D@BD +@HWI-ST575:165:C1P5BACXX:1:1101:2802:3618 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGHJJJJIJIJIIIHIGIGIIIIJIIJIIJJJIIJJIGIGIIJJJGJHGFDBEEDDDBDDDDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:3064:3520 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHFFIJJIIIIIGJEHIIJEIFHIJHIGIHGHJIJJ;>CCCCD;55;:3 +@HWI-ST575:165:C1P5BACXX:1:1101:4225:3617 +GAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +DDBHHHIGEGEAHIGIJJIIIHFHHGGGFHGGGA@;5AEEAADDBBCCC@CCECC>>>C@@C:C9<:A:@8 +@HWI-ST575:165:C1P5BACXX:1:1101:4165:3628 +TGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHGJJJJJJJIIJJ1CFHIJGGJJIJIJIJIGIJJIJJJJJJJIJHIGEFEBCE3>B +@HWI-ST575:165:C1P5BACXX:1:1101:4951:3655 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGG ++ +DDHDFFEGHIDHEHFEG;FIEHGE@GGIEHIGGI3CGEGG>9=CEEEH>>B>;@ +@HWI-ST575:165:C1P5BACXX:1:1101:4878:3725 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHIJJJJJJJJHIJJJIIJIJJJJJIJJJJHIGIIJJIIIJJIIJJJHHDDDEED +@HWI-ST575:165:C1P5BACXX:1:1101:5530:3596 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGDFHHIJJJGIIIJHEHIIIJJJJIIIJJJJJJJJJJIGJIIIJJJIIHBD<AFCCHIHHB +@HWI-ST575:165:C1P5BACXX:1:1101:9213:3506 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACA ++ +FHFHHHIGGIIJIIDHGHIIGIIIFIGFEDGGIIIIJIIJIIIJADGHGGFE>C@BC(; +@HWI-ST575:165:C1P5BACXX:1:1101:10414:3701 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHFFHGIIJJJJJJHIJDFIHIGGIJGHIJIIIHHJIIIIIGHIIJHEHIHHFD?A(=ADDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10901:3561 +TCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCA ++ +FHHHHHJJJJIJIJFFGCIHIGGIJIAEGHF@GHIJIGGGGGFGBHBDADBDDBBD@DDDBBBDA +@HWI-ST575:165:C1P5BACXX:1:1101:10915:3580 +AAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC ++ +FHHGHHIHIJJIIJJIJJIJJFHIJJJJIJIGGHIIJIJJJIJIHHHHGHFFFFFEC?BDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11184:3661 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +BC</'31;; +@HWI-ST575:165:C1P5BACXX:1:1101:11415:3524 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHGHHHGIJIJIJJJHGIHIGIIJIGIGIJIJGIHIGHIGIIIJIJHHHFFDCBD?AC?B +@HWI-ST575:165:C1P5BACXX:1:1101:12086:3581 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJJJJJJIIJIJJHJJJJJJJJJJEHJJIIGJJJJJHHHFFDDB?@>CB +@HWI-ST575:165:C1P5BACXX:1:1101:12420:3632 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTAGAGGCTGACGGCAG ++ +FD;FDFEHIIII@GFHI;GGDHGB@FEHIIFEHHIIEEGA4@FFC;CHEDBB +@HWI-ST575:165:C1P5BACXX:1:1101:13315:3747 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCT ++ +ADADD<@+GIHHFHGDFFFFECDDD?BB8?(+ +@HWI-ST575:165:C1P5BACXX:1:1101:14952:3691 +CCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGAC ++ +FFHHHHJIJGHGIIJJJFHHJGHEHHIIJJJGEGGDGGAG?FHGGIGIIJIIJJIIH=EDFCDCDD>?B +@HWI-ST575:165:C1P5BACXX:1:1101:15058:3596 +CGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +FHHHHHJIIJJJJJIGJJJJJJJJGIJJJJJFGJJJIJDHHHHHHFDEFFFEEEEEEBCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17467:3686 +TTAGAGGCTGACGGCAGGAAAAAATCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCA ++ +FHHHHHJJJIIIIIJJJIEGIIIJGHIJGJJIGEIGIIIJJHHHHGFFFBDCAAEEC>@CCCCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:17669:3607 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHFHHHJJJJJJIIHIJFGIHIJGJJJJJJJJJIJJJJIHGHHFFFFFDDDDDD@BC99@78ACDCD +@HWI-ST575:165:C1P5BACXX:1:1101:18356:3623 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJIIJJJJJJJJGHGGIJHJJJJJJJIJIJJJJJJIIFIJJIIHHFFDDBDC=A??ABCDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:18913:3633 +ATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHHFHIJJJJJJJHIIJJJJJJJJJIIJJJJJJJJHHFFFDDDDDDDD/CCCDAC9< +@HWI-ST575:165:C1P5BACXX:1:1101:1773:3939 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +DFH@FHHIIBHBGIIDFCDCBD8BDDDACDDB@ +@HWI-ST575:165:C1P5BACXX:1:1101:4430:3860 +CATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ 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+GGTCGTGCCTAGTGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +FHHHHHJJJJJJHGIJJIJIIJJIJJJJJJJJJIJIJJJIGJJIJJIIGJJGFFDCEECDDBBDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:12790:3907 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DHD?FF@FGIGBFFEHGBFB@BBCCC@A +@HWI-ST575:165:C1P5BACXX:1:1101:15156:3801 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHDHHIJJJJJJIJJJFHJIGIIIJJIJCHIJJIJJJJJJJHHHHFFFFDDDDD?BAC +@HWI-ST575:165:C1P5BACXX:1:1101:16559:3892 +TGCCAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +DDCDHF:ECFGBHIEEEGFBFCB?DFFDD:??BFHDD=FAFEGB@GF;?>?=?D<>B@>=?&9<<< +@HWI-ST575:165:C1P5BACXX:1:1101:17255:3956 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHFHHIJJJJJJIJJJJHGIIIIIIJJJJIIJJGIJJJJIGJJJJJFIHHFFDD??35 +@HWI-ST575:165:C1P5BACXX:1:1101:17500:3790 +TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +DHDHHDHIIJIIHGI;FIEIGIIJIJJJJIJG@HIJGIIIJFHHGFFCDDDD@ACCD7@B8CCABA +@HWI-ST575:165:C1P5BACXX:1:1101:18440:3926 +CCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACT ++ +FHHHHHJJJJJJIIJJJJJJJJJJDGHJJJJIIJIJHGJJJJIJJJIJJJJJIJIJIJJ +@HWI-ST575:165:C1P5BACXX:1:1101:19111:3987 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHAFHHIIIJJJJIGIJJJGIAGGGHIJIIJGIGJJJIJIJJIIHHHGFDDDDD??CD +@HWI-ST575:165:C1P5BACXX:1:1101:19557:3888 +TTGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHHFDEGIGIGIJGIIDGGG +@HWI-ST575:165:C1P5BACXX:1:1101:19920:3801 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHHJIJJJJIJHIJGIIIEHGIJIJJJJJJIIIJJJJIIJJIIHHHFFDDDD@CC5;@??CCCBDADDCDB88 +@HWI-ST575:165:C1P5BACXX:1:1101:1580:4241 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHFHGHIJGJIIHIBGGHDGGEHGIJEHIJGGHHGGIIIIHIDIJIGHHHFCDB??CCDD?EECDAB?CBB +@HWI-ST575:165:C1P5BACXX:1:1101:4437:4204 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGHHHHIIJJJJJIIIJJJIJJJGGJJJIGIJJJBFHIJJIIJJIJJHIIIHC@==>?( +@HWI-ST575:165:C1P5BACXX:1:1101:7890:4076 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTTACGGCAGGAA ++ +FGFHHHJJJJJJEHIGGHIIICHGCFGHHGHGIBGCGHIGIJDGHIHEEEFBAB7= +@HWI-ST575:165:C1P5BACXX:1:1101:8052:4159 +TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DFFFFBEFEHDA?GID?9?BDD?;C<=8BCFHIH@=DAEBBA:5??= +@HWI-ST575:165:C1P5BACXX:1:1101:8080:4229 +TTTCGGCGGATCCAAATCGG ++ +DHFDHFFEHGFGGEHIIIHG +@HWI-ST575:165:C1P5BACXX:1:1101:8526:4228 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJIJJJJJJJJIIIGGIJIJIJJJIIIJIJIJJJJJJIGHIIJHHFFDDBDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8765:4049 +TAATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHGHJJJJJJJJJJGIIJJIIIIJJJJJJJJJJJIFIGJGHGEHHHFEFDFDCA?1?BDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9822:4042 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHFHHJJJIJJJIJJJJIIJIGIIJJJJIJJJIJIIJJJIJJJIIIIGIJHHFDAC3AB +@HWI-ST575:165:C1P5BACXX:1:1101:10184:4066 +GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJIJJJJJJIIJIIJJIIJJIJJJJIJJIIJJJGECHHFFDDDBBCD9 +@HWI-ST575:165:C1P5BACXX:1:1101:11794:4113 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAGCCTACGT ++ +FHHHHHIJIJJJGHHIIHGGHGIIJJJJJIJJIGIGJJJJIIJJJJDGHFFFDDDBCDD?2?BB?@DB +@HWI-ST575:165:C1P5BACXX:1:1101:15267:4200 +ACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCG ++ +FFHHHHIJIJIIIIJIGIJJJFFGHGHFHIJIIIFJJGGIGHHIIHGHFEFD>BBD?BBBDDDDDDDBDDDDA::+ +@HWI-ST575:165:C1P5BACXX:1:1101:17040:4246 +GAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAAC ++ +DDFFHBGGHIGGIIIIIGIGHHIHIHHIGCHIC=@ +@HWI-ST575:165:C1P5BACXX:1:1101:17659:4106 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHGDHDFIIIIHCHHEG6BHHIIIIGGIIHIIG;;@GIIIHIDCEIHHEDDDDD=?C?<=@?A@ACBDACDDDD@? +@HWI-ST575:165:C1P5BACXX:1:1101:17880:4233 +GGGTCGTGCCTAGCTAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGG ++ +FHHFFHHHAEDGBCFEGIGBBBC +@HWI-ST575:165:C1P5BACXX:1:1101:19348:4134 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHDHHHJJIJJJJIIJIIJIIBGBEFFEGEIGGHIJJJJJIJIIIIIJHHF:BABBDC +@HWI-ST575:165:C1P5BACXX:1:1101:1929:4262 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJIJJIJIIIJHJIJJIJJJJJHHHHHHGFFFFEDEDBDDDDBBDDDDD?BDCDBD +@HWI-ST575:165:C1P5BACXX:1:1101:3262:4389 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +??FDFFGD>FGHHBGCFHEGCH9@D@ACEBEE>FGIGFFB:B==?A +@HWI-ST575:165:C1P5BACXX:1:1101:7054:4268 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHFHHGIJJIJJJJJIJGIIGEIGIIJJIJJJJJJJIGGGGIIIIGHHDDCCDBBDCC +@HWI-ST575:165:C1P5BACXX:1:1101:7021:4304 +ACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +FHHDGHIJJJIJJJIIJGIHIJJJJHHIJJIJGIIJJJJCCEAHCHB?CFC@CEEDEEDDDDDB +@HWI-ST575:165:C1P5BACXX:1:1101:7330:4272 +TTTAAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCT ++ +FFHHHHJIFHIHJIIJHHHIJGHJIIIIGIHJJGDHICG?FHEIIGIGIGIDGGEEECCECCDD +@HWI-ST575:165:C1P5BACXX:1:1101:7985:4412 +TTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGTGAGAGTGGAACGCGG ++ +FHHHHHJJJJJJJIJJJJJJJIJJJJJJJJIGIFIJIJIJJJJJJGIHIIJIJEHIGGH +@HWI-ST575:165:C1P5BACXX:1:1101:9154:4399 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGG ++ +DAA0CDHE?H@HGGI@EC +@HWI-ST575:165:C1P5BACXX:1:1101:9397:4390 +TTGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGGHFHJIJJJIIJJJJJJIIJIJJJJJJJJJJJJIIJIJJJJJBEGGIIIHHFFDD@CC +@HWI-ST575:165:C1P5BACXX:1:1101:9626:4444 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGHHHJJJJJJJJJIJJIIIGIIIJJJIJJJJJIJJJJJJJJJGHHHHFFDDDDB@>CDDBBBDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9920:4375 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAG ++ +FHHHHHIJJJJJJJIJIGHIIIJIJIJIIJJJJCGGIIIJIIJIJIJJHGF>CDD@C(;@? +@HWI-ST575:165:C1P5BACXX:1:1101:9928:4484 +GCGAAGTCATAAGCTATGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTC ++ +FGGGHHHHGIHIIJJJJJJIJJJJIIGGIGEHJJEIJIIHIGFFFCECDB?ACCDBDD +@HWI-ST575:165:C1P5BACXX:1:1101:10347:4290 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHHJIJJJJJJHIIIJJIGJIIJJIGJJJJJJJIJGJJJIJJJHHHFFACDBBDCBDB@@CDD;A@CBDDB8< +@HWI-ST575:165:C1P5BACXX:1:1101:10607:4474 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJIJJJIJJIJIIIJGIIJIJFIJJJIIJJJJJJJJJJJHFHF>ADBBD(< +@HWI-ST575:165:C1P5BACXX:1:1101:11109:4422 +GCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGA ++ +D?CFHBACEE@EEHBH?9AECEDDDBD;8 +@HWI-ST575:165:C1P5BACXX:1:1101:11803:4357 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHFIJJJIJIJIJIHHIIGJGIJJJIIIJJIIIJIIJJJIIGGFHHFDD@BBBCDBBB8A@A +@HWI-ST575:165:C1P5BACXX:1:1101:12622:4263 +AGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +FDFFHFIIIIAFHGEHEIGIIIGII9CFHGEBFEGHHGIGI@FH;)8CBHEED?D@?=B@BB +@HWI-ST575:165:C1P5BACXX:1:1101:13888:4376 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FGFHHHJGIJJJFGFHIHIIIJJGIIIIHIIJJGGIIIIBGGFIHHGHBDDDBD@BD?B +@HWI-ST575:165:C1P5BACXX:1:1101:14985:4327 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGCCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHFHHIJJJIIJIIIJIGIIIHIHJIIFGGJIJJJJJJIIFHGHEFFFDDDDDD?BC?5 +@HWI-ST575:165:C1P5BACXX:1:1101:15294:4416 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTA ++ +DFHCFF@HGGHIICCFHGDHHHEDBE@F@>=B;A=FHGGI +@HWI-ST575:165:C1P5BACXX:1:1101:16020:4286 +GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJIJJJJJJHIIJJJJJJIJIIJIJJJJJJJIJJIIIHIIHGFFDDBBCDB +@HWI-ST575:165:C1P5BACXX:1:1101:16371:4398 +CCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGHJIJIGIHHIJJJJJIIIJIJDGHJJIGIFJIJJIIIJJJHHFFFF@BBBDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17919:4354 +ACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +BFHHFFIIIHIDHGJIIGEHIHE@FHF:CCCGGGIDFFBDGCA;@@AAEEBA:/=BACCCBB@B=@ +@HWI-ST575:165:C1P5BACXX:1:1101:20762:4415 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +DDADF8CBHEEGEGHAACGGGCFAEAHDGGIEG8BFHGEEHHGGAED;8;B +@HWI-ST575:165:C1P5BACXX:1:1101:21005:4492 +GGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FGHHGHIJIJJIJIJJIJDIIJJIIJJIJJJIIH>DGGHGHFEFCBC?BBDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:2208:4721 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGTATCCTTGAAAG ++ +FHFHHHGJIJGJIIIIJJJCICGGHIJGIIHIIGIGHIGIIHCHHIFCHFFDDB;>CCDEDCD3>> +@HWI-ST575:165:C1P5BACXX:1:1101:3594:4541 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHGHIJDGACEAEEGHEBFEGEGHEHHHHEHGHHGFHGGGGHCCC=BDF???15:9? +@HWI-ST575:165:C1P5BACXX:1:1101:4453:4680 +TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACG ++ +DHFHFGJIJJIJIGIFHGEHGHIIJJJJJJJHGIJJIJJJJIIIHHHGFFDD?=>CDDDB@CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4574:4514 +TTGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAT ++ +DFHHBFHIJJHHIJIIGIIGGIJJIJJJJJGHJEAE?>BDDDDD@DDBBBBDDD@CD +@HWI-ST575:165:C1P5BACXX:1:1101:4512:4554 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGFHJJJIIJJIHIIJIIIIGIJJJJFIJJIJIIJJJJIJIJJHGHFF@BDBBDCBD@BDDDDDB +@HWI-ST575:165:C1P5BACXX:1:1101:6091:4740 +GGGAAAAAAGCCTACGTCTTCGGATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC ++ +DHDHHH;E@C;CEFHHBGGIEDB=FDFGHIIHGDCHGGGIEHGICHCEEFGH;CDEF?BCBC +@HWI-ST575:165:C1P5BACXX:1:1101:7755:4724 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +DFA@DDHIBDGGGBG@A@?FHBHIGCFGE>GHHB@GHIIIE@;=@A?=CEE'=@@EEFFDC>/=8:BBA05 +@HWI-ST575:165:C1P5BACXX:1:1101:2354:4948 +GGTCGTTCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FDDFDHIGHGHIJJIGGHJGIIJGIJGHGHJJJGIGIIEGHEHFEHFBDDDDDDBDDDDDBBDDD?@A +@HWI-ST575:165:C1P5BACXX:1:1101:2921:4941 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FGFDFHHIIIGHGGIGEHHIJ@GHIIIJIGDIGIH>FGGGHEEEEEF>EDFDD?B@?C +@HWI-ST575:165:C1P5BACXX:1:1101:4132:4919 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGHGHGIJJJJJEHGJJIIJHIIGCHIJIJJJJJIJIJJIJFEFFFFFEDD@BD@DD??D:DDDHIIDIIEAGEHAEEE +@HWI-ST575:165:C1P5BACXX:1:1101:6248:4986 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGHFHHIJJJJJJJJGIJJJGIIJJJJJIJJJJJJJJIJEHEHHFFFFEDDDDDDB@@CDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6936:4836 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHJJJJIGFHIJJJJIHGIIJJIIIGGIJJJJJJJIJIBEHIJHEFDCCB?BAC +@HWI-ST575:165:C1P5BACXX:1:1101:7709:4867 +AGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAA ++ +FHFHHHJJGJJJIJJJIJIJIGIII?FGIJJFHIIJIDIJJIGGAHIHIHEE>DADBBDDBBBDBB;<9@@BDD>A +@HWI-ST575:165:C1P5BACXX:1:1101:9439:4978 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGHJJJJJIJJJJJJJIJJJIJJJIIJJJIJIJJJJIJIJHHHHFFFDDDDD@B?CDDDBDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9572:4907 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHFHHHJJJJIIIIJIIGIJIIJGGJIIJIJIJJIJJIIJFEIGIJJHGFF:BDBDA( +@HWI-ST575:165:C1P5BACXX:1:1101:9900:4892 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FGHFHHJJJIJIJJJJJJIIJGIIHIIIGIIIJJHGIJJJJIIJJJIIIJJHFFDDEACB +@HWI-ST575:165:C1P5BACXX:1:1101:9950:4989 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGHHHHJJJJJJJJHHJIJIJGIHHJJJIJJJJJJJJJJJIJJIIHGIGHFBCDDDD( +@HWI-ST575:165:C1P5BACXX:1:1101:10290:4901 +GACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGC ++ +FHHHFGGFHIGIHIIJGBFEGI?FHGHIGGBHGGIGIGHHIGHGGHHFEFDDDDDDBBD +@HWI-ST575:165:C1P5BACXX:1:1101:10794:4755 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHFHHIIIJJJJJJHIIGIJIJGIJDIIIIIGJHGIJIJJGGIJJJIJEFFFDDBD( +@HWI-ST575:165:C1P5BACXX:1:1101:11203:4970 +AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGGFHHJJJJJJIJJJJJIJJJJJJJIIJJIIIIFHIJJJFHGIHIIGHIHHFFDDD3=ABB@BDCCBD +@HWI-ST575:165:C1P5BACXX:1:1101:11597:4816 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHFJJJJJJJJJJJIIIJJJJJJJJIJJJJJJJJJJIGIIIIJDGHGFFDDDDDCDDDBDDCCDB +@HWI-ST575:165:C1P5BACXX:1:1101:11814:4971 +CCGGTCGTGCCTATCGAAGTCATAAGCTAGTGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHGHFHJJJJJJIIGIIJJIIIJIJIJJJJJJJEGBFGIJJIEIJIIJIJJHH<>CA?CCD +@HWI-ST575:165:C1P5BACXX:1:1101:12815:4804 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHFHGDHIIJIJGHIHGGHGIGJGIJJJIIIJIIIIIJJJJGJFII;DAHBECDDBC +@HWI-ST575:165:C1P5BACXX:1:1101:15064:4795 +TATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHHHJJJIGIJJJGIIIJIJHIJIHIJJIJJJJIIIJIJHFFFECEDEDCDDDDBDBCCCD +@HWI-ST575:165:C1P5BACXX:1:1101:15338:4874 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FFCFHDHGHIJJIJBHIFHIIIJIJJEGFFEHIF9CFGHI@GGIGEFHHFDDDDB=C?B +@HWI-ST575:165:C1P5BACXX:1:1101:15739:4984 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +DAAACHDGGGIICG?FD?FGH@@GBCBB2.=CF=@D@CH;==@A==?A89?5< +@HWI-ST575:165:C1P5BACXX:1:1101:17879:4904 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +FHGHHGIJJIJJJJJJJIGGGIIJJJJIJIJJJJIJIJJIGHIIIJHHHHFFDBD?A +@HWI-ST575:165:C1P5BACXX:1:1101:18072:4949 +AGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGT ++ +FHGHHHJGIIJJIJIJJJJJIJIII@GHDDGGGIIIGIJIIIGEHHHF?BAEECDDD=B; +@HWI-ST575:165:C1P5BACXX:1:1101:18453:4916 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHFHHGGIIIIIHEFHFHGHIJIJIIAHIIIHHIIGIIJIJIJIJHHHEF@CB==?(9 +@HWI-ST575:165:C1P5BACXX:1:1101:19254:4780 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGGCTGACGGCAGGAAAAAA ++ +AFADFHGGFGIIEHAAG?GEHGEGBGDGEGGGIF>@CFFHIEEIHHHFFCBCB@?5?B +@HWI-ST575:165:C1P5BACXX:1:1101:19861:4798 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJIJJJJJIJJJIIIGHGIJIIIJJIJHJJJJIJIIJJIIIJHHFFDDBBDD=BBBBC@CDD +@HWI-ST575:165:C1P5BACXX:1:1101:19870:4937 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHIGIIJJHIGGIFHIIEGGEHGHIIJJIIGIIJIJJIGIIIHHHFFDDDD?CD +@HWI-ST575:165:C1P5BACXX:1:1101:19796:4989 +AAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FGHHHHJJJJJJIJJJJJJJJJJJJJJJJJJJJIJJJJHFEFFFFDEEEDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20053:4972 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FGHHGHIJJJGIIIGHIHIJIADGHIHHGIJJJJJHIJIIHIJIIIIJHGFDCDBBDD????ACCCBD +@HWI-ST575:165:C1P5BACXX:1:1101:20782:4908 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHFHAHIIIIHICHE0DGGII>?DFDGGHIIEGAGIGIGIGIIBGE;CBDD@BDBB?C +@HWI-ST575:165:C1P5BACXX:1:1101:1189:5021 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTC ++ +AF22<@F@?CFFCF>EG7CDGCCBDAGHGFDFGB> +@HWI-ST575:165:C1P5BACXX:1:1101:1686:5115 +GGTCGTGCCTAGGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGCCAGGAAAAAAGCCTACGTCTTCGGATA ++ +FHHHHHJJJJFHIJIJJJJJJIJJIGHIJJJIIJIJJJJIIIJHGHIG'=EDF?>;AEDD@BCCDDDDDDDDB?<< +@HWI-ST575:165:C1P5BACXX:1:1101:2938:5127 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +?6CDHFIEDHHDHHGHHBHGGGIIGGGGEFHCECFEA>F;=@AEHD>DAEB<83=;? +@HWI-ST575:165:C1P5BACXX:1:1101:3405:5036 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJIJIJJJJJJJJGIHIIIIIJIJJJJIIJJJJGCIIIJIGHFEDD?BDDBB=BBDCDBB +@HWI-ST575:165:C1P5BACXX:1:1101:7045:5134 +GGTCGTGCCTAGCGAAGTGATAAGCTAGGG ++ +D=DD8@@=EC<339:*?:? +@HWI-ST575:165:C1P5BACXX:1:1101:7123:5216 +TTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATG ++ +FHHBHHJJJJIJIIJEHFEGIJJIIIGIIHIGIJGIGDGGIJJJJJJJJIJJIJEHFHG +@HWI-ST575:165:C1P5BACXX:1:1101:8358:5145 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +FHGFDHEHIJHEHEFBDCAA@ADDCCDD@BDD@DCDDEDCCCDDDB2B?ABDDDDDDCBDB-22 +@HWI-ST575:165:C1P5BACXX:1:1101:10830:5146 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHGHHHIJJIJGIIJEHIIIIGGGHGEIEHIJJIGHGIIJGHIIJHHGGFFDB@B@?( +@HWI-ST575:165:C1P5BACXX:1:1101:11032:5068 +TCTTGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGAC ++ +BDHHHHIIIIIGIAHHHGIIIHIII@HHCHIIIIGIDEHIIIGGIDFGDEIHAHFFFFBCCCB8? +@HWI-ST575:165:C1P5BACXX:1:1101:11565:5085 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHFHHHIEIGIIGGGGIHIGHGGEEGHGIIIJJHGGGJEHGHJIIHECHFDD?B=A?CD +@HWI-ST575:165:C1P5BACXX:1:1101:12209:5017 +AAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAT ++ +DBAFFFF2AEAB +@HWI-ST575:165:C1P5BACXX:1:1101:12070:5238 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATAT ++ +FHHHHHJJJJJJJJJJJIJJIIJIJJIJJJJJJJIJJJJJIJJJJJJHHFDDD9;?AB:9ACDDBABCBCDB?854 +@HWI-ST575:165:C1P5BACXX:1:1101:12614:5234 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGAAAAAA ++ +FHHHHHGIJJJIJIJIJJIJIIIJIIJIIJBHIJHIIJJIIJJJJHGFHEFDDB@CDDBD +@HWI-ST575:165:C1P5BACXX:1:1101:12934:5066 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJIIJJJJHIJGJJIJGIJJJJIJJIJIIIJJJJIJHHFFD@?ACDBDDABDACB? +@HWI-ST575:165:C1P5BACXX:1:1101:12889:5153 +TAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHHHHJJJJJJJIIJJJIIIJJJJJJJJJJJJJBHIJJJJGIHFEHFFFFFDDDDDDDDB +@HWI-ST575:165:C1P5BACXX:1:1101:13006:5008 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTA ++ +:88<@CD48DDF>;C@F=@@@@E +@HWI-ST575:165:C1P5BACXX:1:1101:13701:5054 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHHJJJJJJJJJJJJJJJIJIJJJJIJJJIJIJJJJIJJJIIHGHGDDDDDBDDDBD +@HWI-ST575:165:C1P5BACXX:1:1101:13818:5190 +TTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTGGCGGATCCAAATCG ++ +DHHHHHJJJJIJJJCBG@HIHIIGIJGIJJJJGBFGFIJJJJJIFDDDDDDDDDDDBDDBDDD@CCCB +@HWI-ST575:165:C1P5BACXX:1:1101:14721:5211 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGG ++ +DFCDF1@AEFEF9CC@FBDBFFFF*9?F<=BDF8BF>FEFEEDCEEE9=B>'9> +@HWI-ST575:165:C1P5BACXX:1:1101:15180:5074 +GTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJJJJJIJIIJJJJJJJJIJIJJIJJJJJJJJJJJJJIIGIJHHFFDDACB 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+FHFDHHIGIJGIIHDDHIIGIEFHIIGGGGIEHFHIIIJJHGIJJIIGIIHGFD?@EC +@HWI-ST575:165:C1P5BACXX:1:1101:21070:5172 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FGDFHHJIHGIIGH@FGDHGGEGHGHGGHGIGIGGGGHIGGIIIIDAGHE<99;??CC +@HWI-ST575:165:C1P5BACXX:1:1101:1152:5446 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DDACF@EGHGGGHBGDHDDGAFDE??>C +@HWI-ST575:165:C1P5BACXX:1:1101:2332:5476 +ACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHHHHIIJJJEHIJHJJIJIGIHIJJHHJIJHIJJIIJJDHDDCDBDDDDBDDBDDDDDD>ACCBB +@HWI-ST575:165:C1P5BACXX:1:1101:3549:5422 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJJJIIIIJGIJIIJJGHGHHFFFFFEEEDCEDDD?CBDDBDDBACDDDDDBDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3934:5255 +TCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGHGFIJJIJJIJJIJJJJIJIJIJJJJJJJIGIJIGJJJIIIIIJJHHFF7>CCEDDD@DDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:3779:5257 +GTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHGHJJJJJJJJJJIJIJJJJIJJJJJJJJJJIIIHJJJJIIGFHFFDDECCBB@BBDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3981:5396 +GTGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +DFDFHFEHBGECHBGGG@??B@?F>FBFFCHF7F/BFCHEGG4@FHH;EHD=49=;2 +@HWI-ST575:165:C1P5BACXX:1:1101:4352:5435 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHIJJJJJIJJJIJJJJJHHJJJJJIJIIIIIJJJJJJIIJIJJHHFEDDDDDDDDDBDCCCBD +@HWI-ST575:165:C1P5BACXX:1:1101:5757:5381 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +D?FFFF@GHIIGIGIIEIDFGIIIGIEGFB@DFIGHGIGIIGEGIIIHIG64'=>;,>3 +@HWI-ST575:165:C1P5BACXX:1:1101:7084:5290 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHGFHHIJJJJIJIJJJJJJIHIJGIJJHGIJJJJJJJJJIJIIHHHGFFFDDDDBDD +@HWI-ST575:165:C1P5BACXX:1:1101:7550:5435 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHFHGIIIJIJJIJEHGHIIGJJIJJJC@GIJIJHIIJJJJHIFHHDFFDDDDD@AC 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+@HWI-ST575:165:C1P5BACXX:1:1101:16958:5440 +AAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACC ++ +FHHHGHIIJIJJJHIJJJJIIJJIIJJJGIGHJJJIFHIJIJJJJJGHIJJIHHHFFFFDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17064:5269 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJHJJIIJIJJJJJJJIIJJJIJIJJJJIJJJJJJIIIHHFDDEC3=@???CDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18711:5374 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHHJIJJJJJJIIJHIIIJJIJJJJJJJJJIJJJJJIIJJJJIIJHHFFDDBAA=;= +@HWI-ST575:165:C1P5BACXX:1:1101:19795:5388 +AAAAGTGCCACAGGGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGG ++ +BCCCCD>CC>A@:>:CC +@HWI-ST575:165:C1P5BACXX:1:1101:3186:5524 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGHHHIJIJJJJIJIJJJIIIJGIJJJFIIJJIJGIJIGIEGIHGEEABDDBBBBDDDDDBBCDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:3099:5544 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ 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+GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FGHDFHEHIIJJJJJIJIBHGIIIGIBHGHIIIIEHIIJJHHGEFFFFFCDDDDD?ACD2 +@HWI-ST575:165:C1P5BACXX:1:1101:9106:5832 +AGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAA ++ +FGFHHHIIDIHHIJGJJJJJJJIJJHIIGEHGIDIHIGIGGHHI@EHIHDHFFFBBBDDDBDBBDDDDDB@<<<:> +@HWI-ST575:165:C1P5BACXX:1:1101:9392:5986 +AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FFGFHHGIGIBGIEIE?FCFEGGFFHGEGFGGGEGFGFGHHEHHFIEDGGEAB/>=A(5 +@HWI-ST575:165:C1P5BACXX:1:1101:9549:5793 +TAAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACC ++ +DDDDDD2CEEEEEFAC:F?CEEEEEEIEE4CDFDEDDBDDDDEIDDDDEDDDD@@DBB?CB +@HWI-ST575:165:C1P5BACXX:1:1101:13983:5888 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHFHHHIFJJIJHHGHIGIJIIGIIJJJJIJGJEHIJJIJIJIJJJHHGFFBDDBDC5 +@HWI-ST575:165:C1P5BACXX:1:1101:13915:5939 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ 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+GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGGCTTT ++ +DF8DFFDGE@EFFG:?D?DDAFIDFFEAECFIF':AEF +@HWI-ST575:165:C1P5BACXX:1:1101:17404:5768 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHGDHHGIIGEHGGJBHJIGIIJIIGIGIHJJGHAHIJJIGIEGGHHGHFFACDD7<:< +@HWI-ST575:165:C1P5BACXX:1:1101:18508:5770 +CGTGCCTAGCGAAGACATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHGHHHJIJJJIIJIJJJIIIJJIJJJJIHGIIJJJIJIJJJJAEHHHDDD?=ACBDDDDDDDBB +@HWI-ST575:165:C1P5BACXX:1:1101:18654:5893 +AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHHJIIJIJJJIIJIIJIJIIJJIJIIJIJJJJJJIJGGIIJJIHIJHEFD?>35?@@9ABBBBBB;A(<5<95DHIDHIBFGCDHFCHEHGGIIHJGIJGGJJFFHHHDE>AB@BBA3 +@HWI-ST575:165:C1P5BACXX:1:1101:4344:6153 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAACAAAGCCTACGTCTTCGGA ++ +FHHF)@GHIJJJIGIIJJJIBGIIIIIJJIJJGIIIIJJJIIJGEHHHHDDDCDDBD(,(5??ADDDDDDCDDDB< +@HWI-ST575:165:C1P5BACXX:1:1101:5212:6077 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DF@FFFHJIIJJIIJII?FDHHIGIJGGGGIGGHHIIIJJGGIGHFHDDB<>BB??C< +@HWI-ST575:165:C1P5BACXX:1:1101:5854:6193 +AGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHFFHEHIJJJJJJIJFHIJFIBHHAAC@CC25 +@HWI-ST575:165:C1P5BACXX:1:1101:6093:6246 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAACAAAGCCTAC ++ +FHHFHHIIEIGEIIIEBHFGBFBHIIJGIGIGHJHGHGGGHEGGGIJ9=ADDBA??B295<>?BAAD +@HWI-ST575:165:C1P5BACXX:1:1101:8105:6158 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FGFHFFHGIJJJIIGIIDFIGIEHHIGIGBFDHIGIIGGGHEGGGEEHHFD?@B7=( +@HWI-ST575:165:C1P5BACXX:1:1101:8253:6223 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGCAA ++ +DF@DFCFFBFFIBCDF:0??DFAGFI<8?FE;FFFE@F@CFE@EE>>CEDC/>:',5 +@HWI-ST575:165:C1P5BACXX:1:1101:8552:6031 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACTGCAGGA ++ +FGA@DFFEGHEH@FGDGGBBDGIGIGGJEGGGIGID=AGIJHHJJG?;CHEEFFF7 +@HWI-ST575:165:C1P5BACXX:1:1101:8575:6101 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +BDDDFHFHEGGGIIEHGGHHGCH@HGGGGBG?GG?@6CFH?B?CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9569:6015 +ATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATC ++ +FHHHHHJJJJJIJJJJJJJIJIJJIIJJJJJJJJIIJJFIIIGH@GIIGIJGGIGIJHFFFFEEDDDD'38;@90) +@HWI-ST575:165:C1P5BACXX:1:1101:10905:6024 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +DHFADFEGDFEEEDGI@:?CFGDGD@FDFB?BF@?DFEFGB<3? +@HWI-ST575:165:C1P5BACXX:1:1101:21261:6248 +TATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGA ++ +FHHHGHIJJJJJBFHJIJIJJGIGFFGEIJJJJEHJJIIIIEG@FHIHIGIAGGDGGGGE +@HWI-ST575:165:C1P5BACXX:1:1101:1089:6262 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FH@FFDGIIIIIGHHFH?FGEGGIFG>GGHEHFBGHEFGFHIG=;AAHEFFBBB?ACCAB +@HWI-ST575:165:C1P5BACXX:1:1101:1439:6319 +TATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +2C?< +@HWI-ST575:165:C1P5BACXX:1:1101:1829:6451 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGATGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJIGHEFFHGEFGIIIIIJJIJJIJIJIJIIIJIJJJJJBHIJJGFF?@CCB@D??CC?=A +@HWI-ST575:165:C1P5BACXX:1:1101:2484:6444 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +DFFAFDFEFGGIIIGGBBFGIJEGIIDD8FGGE8==CH@@D=@E@AAEHEBB<;5;? +@HWI-ST575:165:C1P5BACXX:1:1101:3350:6364 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGC ++ +FHDFFHEDEEFGHIIGB0?A9BAFFAGGHE@AFHGAGE>AAEHH +@HWI-ST575:165:C1P5BACXX:1:1101:3699:6336 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGA ++ +DDDFHHEG;FHI>F?F@?CFGEEH>D@(@B/=>>8( +@HWI-ST575:165:C1P5BACXX:1:1101:3874:6280 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGATGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGA ++ +FHHHHHJJJJJJJJJJJJJJIIJJJIJJJHIJGIIHJJJJJJJIJJHHHHFFDDDDD(99@BDDDDDDDDDDDDD< +@HWI-ST575:165:C1P5BACXX:1:1101:4404:6415 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFHHHJJJJJJJJGIJGIJIJJIJJGGGIJJJIJJJIJJIJJJJJ;EHHFCDD=BD39 +@HWI-ST575:165:C1P5BACXX:1:1101:5416:6451 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACA ++ +FHHGHFGHIJIJIJIIE@FFIIIIJIJGFIIJJIJJIJJJIGJJCHCEHBHFFDDBE(> +@HWI-ST575:165:C1P5BACXX:1:1101:7066:6455 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAA ++ +FHHHHHJJIJJJJJIJJIJJIJIIJJJJJJJJJIJJJJJJIJJIIJIHHHFBDD@AC +@HWI-ST575:165:C1P5BACXX:1:1101:7488:6251 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGAT ++ +FHHHHHJJJJJJJJJJJHJIIJJIJJJJJJJJJIJJJJJJJIHHHFFFFDDBDDD?C??B>BDCDDDDBCDCDD?8 +@HWI-ST575:165:C1P5BACXX:1:1101:7486:6329 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHGHHHIIJJJIJIIJEHGHIIJJGGJJJJIJJJGIIJJJJGGIJIIGJJIHBEDA@C=B +@HWI-ST575:165:C1P5BACXX:1:1101:7643:6450 +ACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHHFHHGGGHGIGJGBHGHIGHIIJEGHHEFFDB@D8A@CCCCBB +@HWI-ST575:165:C1P5BACXX:1:1101:8454:6487 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHFHHIIIJCHHGGHGGGHHFHFHEHJJ@FHIJIJICGHGHIGIJHEEFFDFFEDCDD@>CDD +@HWI-ST575:165:C1P5BACXX:1:1101:9137:6329 +AGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHHJJJJJJJIIJJIJJGIJIIIJJIFJJJIJIJJJJJJJJJJIJJHHFCD??;? +@HWI-ST575:165:C1P5BACXX:1:1101:9587:6277 +GCGGTCGTGCCTAGCGAAGTAATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHFHJHJGIJJJIJIIJFEHIGICHIIGGIFIJGIJIJJGHHDFFFFFDDD?D@?5>AB +@HWI-ST575:165:C1P5BACXX:1:1101:9536:6348 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHDHDHIIGIJJIJHIIEHIHGGHDGHEGHGGHHGIG@C=GIAHHGEHFHEDCB=55,9 +@HWI-ST575:165:C1P5BACXX:1:1101:10054:6289 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHHHHJJJIIIIJJIJJHIGHIGIJJJHIGIJGHGGIJI@HGGJJGGHFDDDDD;>,9 +@HWI-ST575:165:C1P5BACXX:1:1101:10173:6294 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHHJJJJJJJJJJIGGIHJJIJJGJJIJJJJJIJIJJIIFIJJIIJJHHFD;6;(;; +@HWI-ST575:165:C1P5BACXX:1:1101:10764:6333 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHFFDDEGBFFHHIGGGHGHEHIEFGHIGBGIGEHHIIGHCCHHFFFDBCBBBB155(95 +@HWI-ST575:165:C1P5BACXX:1:1101:11047:6477 +GCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +BDFFFBGIGFFGFGHIEIIIG@HG@:GEDBHGIGG;5@;=@E;AEE@DFDCCC6A@CB +@HWI-ST575:165:C1P5BACXX:1:1101:11411:6372 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFFFHJJIIGIIJIFHEFCHHGHGIGGIFFHHFFFFFFEEDEEDDDDDBBD@BD?ACC? +@HWI-ST575:165:C1P5BACXX:1:1101:11665:6448 +GGTCGTGACTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHFHHFIEIJHIHHHIIIHIHIJIIJHIJJIIIIIJJJIIIJIIGIHHHHFDDD@ACDDDDABC@<@? +@HWI-ST575:165:C1P5BACXX:1:1101:12774:6457 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FGH@FFDEHIGCHGHGHIFHHIGJEHIJJGIJGH@@DGGGIGHIBCEHCBBD>>@=(:?5 +@HWI-ST575:165:C1P5BACXX:1:1101:13106:6369 +AAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTC ++ +FHHHHHJJJJJJIJGJJJJJIIJJJJJJJHIHIJJJJJJJJJJJJJJIJIJIHHHHHFFDE +@HWI-ST575:165:C1P5BACXX:1:1101:16704:6321 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHFHHIJJJIIJJJJIJGIJIJJJIJIJIJJJJJIJJJJJJJJJIJIIIHHFFDDEDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16660:6380 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHFHHHJJJJJIHIGIIGGCHGIJIIIIJJJJJJIJJJJJEIJJJJIGIJHHFD5@CCB +@HWI-ST575:165:C1P5BACXX:1:1101:17964:6393 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAA ++ +FHHFFHIBHGGEIHHFHIGGHHC>FBGIGIJI7F@DFHGIEGCHGEHGFFFDDDD5? +@HWI-ST575:165:C1P5BACXX:1:1101:18173:6282 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHFFHGHIJJJJJJHGJEHJIJJJIJJJJJJJIHIJJJJJIJJJJHGFFFDDDD=AACBDB1AEDC@C +@HWI-ST575:165:C1P5BACXX:1:1101:20043:6415 +ACGACGATCTTCCGATCTCGGACACAGACGG ++ +DDDAFF6DEFIIFIECG>FFI>FIFFEBE'5 +@HWI-ST575:165:C1P5BACXX:1:1101:1982:6500 +ACGACTCTCTTCCGATCTGGACACCGG ++ +FHHG?FHIEGIH@GEHIIIJJIJGCHH +@HWI-ST575:165:C1P5BACXX:1:1101:1934:6651 +TGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +DHFHHHIJJJJIGIIHIGIIIJJIJIJGIJIIJJJIJJJGIIFIIIFIJIHFFDCCDCDBBBBDCCBD +@HWI-ST575:165:C1P5BACXX:1:1101:3031:6587 +ATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHHGHHJGHJJJJIJIJJJJIJJJJJJJJJJJGIGIIIIJIFHHHHFFFEEDCD:B?BDCCD +@HWI-ST575:165:C1P5BACXX:1:1101:3659:6632 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHFCFFEHJIIIJIJGGIHHGIIJIGIJIAGH?HGIIJJEIGGEHHHFFFDDBBB?B:5(0 +@HWI-ST575:165:C1P5BACXX:1:1101:4271:6639 +GGCTGAGTATCCTTGAAAGTGCCACAGTGACGAAGTCTCACTAGAAATGGTGAGAGTGGA ++ +FGGHHHJIIJJJEIIIJJJBHGIIICHCFGIJE?GDH9FHHJJI)=8@FAFFGIEHGIH? +@HWI-ST575:165:C1P5BACXX:1:1101:5521:6516 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAACAAGCCTACG ++ +FHFHHFIIJJIGEHHHBDGFIIIIJIGIIIJIFGIIJJJIGCHIIIEHH>BCDB?BC3?(,98C>CB +@HWI-ST575:165:C1P5BACXX:1:1101:6888:6616 +GTTAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGA ++ +DADFB<+C?GBH?GFGF8CDF +@HWI-ST575:165:C1P5BACXX:1:1101:15075:6709 +GGTCGTGCCTAGCGGAGTCATAAGCTAGGGCAGTCTTTAGAGG ++ +DFFFFDGBGB?ECF)0?0:9BGG<9B>3DF8BF.8@@F>@C>@ +@HWI-ST575:165:C1P5BACXX:1:1101:16741:6696 +GATCCGTTCGCATTGACACTGCTTATACGTGTAGACGG ++ +FHHGHHJIIJJGJJIIJJGIIJJJJJJJJJJJJJIJJJ +@HWI-ST575:165:C1P5BACXX:1:1101:16765:6674 +AGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAATCGG ++ +FHHHHHIIIJJJJJHIHIJJJJJJIJJJJJJHHHHFFDDDDDCDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19358:6651 +ACACGACGCTCTTCCGATCTATGATACGCGG ++ +DFFFFAA)@FFEC@F:?BD>?DF@FEECFAF +@HWI-ST575:165:C1P5BACXX:1:1101:19561:6627 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCA ++ +DDDDDCEDEIEC3C?D@:?DECDEDDDDBDDDA=BBDEEI>@>CDDCD8=?BDBB@859?C?@BBB@(9; +@HWI-ST575:165:C1P5BACXX:1:1101:1927:6913 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHHHHJIEGII@DAF>=BC58@@EFCFEEAEFF>@=@?'3;59>@?@ +@HWI-ST575:165:C1P5BACXX:1:1101:10199:6779 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAA ++ +FHHHHHIJJJIJHGIJIHGGIGIIIJJJJJJJJIJIJJIJIJJJHHHFFFDDDD<5:,<= +@HWI-ST575:165:C1P5BACXX:1:1101:11749:6891 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FFFFFHJIJJIJIJHIJIJJIIJIGIIJJJJIJIIIJJJJIIGIJIJJHHFDDC?ACDB?@?ACDDBA +@HWI-ST575:165:C1P5BACXX:1:1101:12769:6834 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCCGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHJJJJJJJJHIIJJJIJJJIJJJJJJJJJJJJJJIIIJJJIIJHFDDDDDBDDDDD@BDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13711:6990 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAA ++ +FHHDHHGIGIIFIIIJJIIJIGJJEIJJJIJIGJIJIJJJJDGIIJJIIEHFDCDB?CD? +@HWI-ST575:165:C1P5BACXX:1:1101:14111:6893 +GGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHHHHIJJJJJJIIJJHIIIJJJJJIIJJJJJJJJJJJJIJIJJJHHHFFDDD?BDDD6=A@CCDDB +@HWI-ST575:165:C1P5BACXX:1:1101:14440:6994 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAA ++ +FHHFHHHIIJGIIIJHJJHIJIIJIJJJJJIJJGFHIJJJIGHIJHHFHFFDCDDBDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15694:6869 +ATGGTGAGAGTGGAACGCGGTAAACCCCTCGACCGATCCGCTTCGGCGGATCCAAAC ++ +DFFD??>FHICHGHHIIIIEDHHGHHIIIIHIGFHHBDECCCBBBBB?85@BCC(:< +@HWI-ST575:165:C1P5BACXX:1:1101:16093:6801 +TGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAACAGCCTACGT ++ +FHHHHHJIIJJIJJJIJJJIJIGIJJJJJJIJJIIIJJIJIGHJJJJGIIHHFDDDB>A(=@FGGIEEEDFBAB;ACC< +@HWI-ST575:165:C1P5BACXX:1:1101:17036:6768 +GGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGCTGAGTATCCTTGAAAGTGCCACAGTGACG ++ +FHHHGFGIIIIGIIHIGIJJIIJHHFGFFFFFDCDDDCCCDCBBDDDDDDDDCC3>AC@ACACBDCCCCB +@HWI-ST575:165:C1P5BACXX:1:1101:17542:6830 +GGGTCGTGCCTAGCGAAGTCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGT ++ +FHHFHHHIJJJJJJJIJJIJJIJFHIJJJJJIIIHJJJJIJIJIHHHHFEDD?BB?B +@HWI-ST575:165:C1P5BACXX:1:1101:1577:7179 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATATGGC ++ +FHGHGHIIIJJJIAHI@GIIH@HCHCEGGG?FGDBHH;BCHGIGAAEECEE9@DEDD?BDACCD +@HWI-ST575:165:C1P5BACXX:1:1101:2297:7148 +TCATAAGCTAGGGCAGTCTTTAGAGGCTGACGGCAGGAAAAAAGCCTACGTCTTCGGATGGC ++ +AFHHHHGIIIIIIIIIDGDHIGHGIIIIIIIIIIBFGBHIIIGHEHHGFFFDCAEABBD?BD diff -r fb76303acf4f -r 431aebd93843 test-data/reads2.fastq --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/reads2.fastq Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,2684 @@ +@HWI-ST575:165:C1P5BACXX:1:1101:1666:1132 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +HIJ0??FFCDB?FGDBAEHHBDDDB?@;8?==@8B87,4::4:(+4@< +@HWI-ST575:165:C1P5BACXX:1:1101:2239:1101 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACT ++ +JJJIGHIGBHHIJJIIGIJH6BDDBBDDBDDDDBBBDBD?CCBDDDDBCCDDDCCDCDCA:@A34(++4+4:: +@HWI-ST575:165:C1P5BACXX:1:1101:2455:1083 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTT ++ +GBGIIGGD@DGHGGGGIAEABBFBDB>B?@=:@BDB<8B9@C8-5<C>>AC4+++++4+44 +@HWI-ST575:165:C1P5BACXX:1:1101:3207:1238 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJHIIJJJFII@EHEEDFABDDDDDDDDDDDBBB>CDBDBDBDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3405:1152 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +GG8:F6B*?3?B;@;;'54==46<>89'5=6,5-0&&0?(:+5;77-?? +@HWI-ST575:165:C1P5BACXX:1:1101:8268:1157 +CCGATTTGGATCCGCCGAGGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJIIIJJGJIHHFDDDBBDDDDDDDDDBDDDDDDDDDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8740:1185 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIJIIJJJJJJJJIJJJHHFFDDDDDDD=BDDDDDDCDDDDDBDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12414:1099 +CCGATTTGGATCCGCCGGAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJJJJJIJJJJJIJJIJHHFDDDDDDDBDDBDBDBBBDDDCBBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14053:1112 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJIJIIIIJJJHHFFDDDDDDDDDDDDBBDDDDCDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14219:1152 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJGIJJJJJJJIJJJJIIHHFFDDBDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDDDDDDEEE:(++4(444:AB +@HWI-ST575:165:C1P5BACXX:1:1101:15012:1211 +CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIGIJIJJIJJIJJIJIIGHGFFDDDDBDDDDBD@@BDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15774:1231 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +IJJHIJJIJJJJJIJJGIIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDCAACED4(+,++4 +@HWI-ST575:165:C1P5BACXX:1:1101:17047:1127 +CCGTCTTGGATCCGCCGAAGCGGA ++ +D@D0?)8*0*/0?86;@';'-=9A +@HWI-ST575:165:C1P5BACXX:1:1101:18461:1188 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC ++ +BC@?FEGGHDDGEGAC@BHHEFEBBBDBDDB'5<<<5:??CDD;BDD@?DDACDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19911:1138 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJBHIJJJJJGIGIDHHGH>DFCD>BDDBDDDDDD7@BDCCBBDDDDCCDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:1311:1500 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIGIJJJJGIIJJJJJIIHHFFDDBDDDDDDBDDBBBD>ACB@BDDDCDDDDDCCDDDDDEEE:4:::4>4::>@ +@HWI-ST575:165:C1P5BACXX:1:1101:2110:1396 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +EHHF@GGG>GHFHIIGIEIBBBBC@?>;8=@BBBB@B8?3@@< +@HWI-ST575:165:C1P5BACXX:1:1101:5495:1254 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJFIJGIIJJIIIIHIJHHFFCDDDDDDDDDDDDDDDDDCDDDDDDCEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:5684:1314 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIJJJIJGHEH*BGGIFHGGIGHHFFFDDDB;BBDBDBDCCCBDDDD?CCDDDCDDC +@HWI-ST575:165:C1P5BACXX:1:1101:6002:1450 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FIIDHHGIFIIJEIICHGIEDFFDD@D@@DD'77B@BDBCDD8DDDDBDDCDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6163:1455 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJIJJJJJJJJIJHHFFDDDDDDDDDDDDDDBDDCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6370:1342 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +?;@:@?;DDD?8/8B;A4A:39353;;50:C()7A=;8283<395BB7::4+>44::8 +@HWI-ST575:165:C1P5BACXX:1:1101:20338:1414 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIG?@DFHFG>F9F@FHGGHFFFC@>>BBBBBDBBB07@CDCB7B@D?ACDCD +@HWI-ST575:165:C1P5BACXX:1:1101:1782:1682 +CCGATTTGGATCCGCCCAGCGGATCGGTCGAGG ++ +GGFH:8?AB=<9A8=8?BBD +@HWI-ST575:165:C1P5BACXX:1:1101:2733:1718 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTC ++ +HGGHGIGGGGHHCHIIIBHBEFFDDB8?<@BBB8BDDB@>@A@BDBBBDDCD?CC +@HWI-ST575:165:C1P5BACXX:1:1101:2796:1586 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIJJIEHIJJJIJJIIJHHFFCDDBBDDDDDCDDDBD>CA8;BDBDDEDDDDDDDDCC@CDE:,+4,(444+:8 +@HWI-ST575:165:C1P5BACXX:1:1101:3075:1523 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIBHIGHIJJJJJIJAGAHFFDDDDDDDDDDDBBDDDCCDDBBBDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4251:1515 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJJIJJJJJJJIJJJHHFDDDDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4481:1718 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJIJJJJJJIJJIIJHHFFDBDDDDDD?BDD@7@DD:ADDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4978:1615 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJJJJJJJJIJJJJJJJHHFFDDDDDDDDDDDDDDDDDDDBDDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6935:1687 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGAH?DBHGIGEHIGIEHEFEBB;@B?BBB:55798?AC:@BDB<@C@CCCCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:7393:1637 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCCCGTTCCACT ++ +IHJJJIAHGIIGE@FGBEGHGFFDDDDDBDDDDBB:79@CCDCBBDDD?CDD +@HWI-ST575:165:C1P5BACXX:1:1101:7744:1585 +CCGACTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCC ++ +B8@@G61D@?*?.81;A/A=6B>:CDD4,+44 +@HWI-ST575:165:C1P5BACXX:1:1101:8452:1710 +CCGTCTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJIJJGGIGFGFDDDDDDDDBDDDD87@CDDDDDDBBDEDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:8575:1513 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +JIIGHJJJJIJJJIGJGGGHHFFDDDDDDDDDDBDB@BDDDCD@DDDBCDCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8610:1649 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIIJJIJIIJIIFGIEHJJJIJHFEFDCDDBDDDBBBDCDDDB5;@B595B<:8?ACB77;B?CDC>ACCAC +@HWI-ST575:165:C1P5BACXX:1:1101:9782:1735 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJJGJJJIGIJEIJHHFFDDBBDDDD?BDBBDDDCDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10892:1546 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTACTCTCAC ++ +HIIBBFHIIEHIDGIJIGEEEFDDDDDDDDDBDDDB@@BDDDDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10875:1631 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIIGIJJJIJJJIJIIJIGHFFDDDDDDDDBDDDB:?ACCD +@HWI-ST575:165:C1P5BACXX:1:1101:17059:1656 +CCGATTTGGATCCGCCGAAGCGGATCGGAGAGGGGTTTACCGAGTTCCACTCTAAC ++ +JJJGIIJJJJIJJJJJJIIHHFFCDDDD@D@DDDDDBBCCCDDDB@@@CDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17823:1711 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJAHIIJIJJJJIIJJIIHHFFDDBDDDDD=BDDBAC?BBDDDDB@B@CDCDDDDD?DEDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20160:1653 +CCGAGAGTCCAGATCGGAAGAGC ++ +JJJJJGJFHIJJIDGGHGIGHGH +@HWI-ST575:165:C1P5BACXX:1:1101:20348:1677 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IGIFHIJJIBBDDBDDBDDDDDDDDDDD;BDDBDEACCCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:1152:1999 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJFGJIJJJJJJJJJIJJHGFDACB@BDBDCDDDDDDD:?CC@@CD4++444>:44>8 +@HWI-ST575:165:C1P5BACXX:1:1101:1575:1782 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC ++ +?DD:FG7DB9>988,8;89B8778?(:><@BBDDGGE@>GBGGEGHFEGEFDD6>>?6?BD=05)579BC@@BD;;@DDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5190:1986 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCACAC ++ +JJJIJGGDFCHIJCGIGGAAA?DDDBBDDDDDBDDD5:BCDCDBDDBDCDDDD3>AD +@HWI-ST575:165:C1P5BACXX:1:1101:7320:1866 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJGIJJIIJJJJJJIIGJJBHFEDDDDDDBDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7508:1798 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJGJIIJJJJJIEGHHFFDDDDDDDDDDDDBDBDDDDDDDDDBCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9647:1841 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGCGTTTACCGCGTTCCACTCTCAC ++ +JJIGHIJJJJIJJIJJIIJGGFFBBBDBDDDBDDD@DDDBCCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11078:1760 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJJJIJJIJJHHFFDDDDDDDDDDDDB@BDDDDDDDDDDDECDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12091:1910 +CCGATTTGGATCCGCCGAAGCGGATCGATCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIIJJIIJJIIJGIJHHIEHDDDDDDDDDDDDDDDBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13182:1885 +CCGATTTGGATCAGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHJJJIJIJJJJIJJJJJJHHFDDDDDDDDDDDBBBDDDDDDDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:13475:1836 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJIJJJJJIJJJJJHHFFDDDDDDDDDDDDDDDDDDDDDDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14275:1813 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJIJJJJJJJIJJJIJIJGHFFDDDDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDDDDCCDED4+++4+: +@HWI-ST575:165:C1P5BACXX:1:1101:14914:1754 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HIIGHJJJFJJDIIJIIJJGGFFDDBBDBBDBDDDBDDDDDDDDDDDDDECDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15826:1814 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACAGA ++ +GHIDGGCEHHGIIIIGHHGHEBD>BBDBBDDBBBBBB9@?CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16316:1995 +CCGATTTGAATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FGG:FDHHGIAFCHIGI@G=:BB<>9@B?BBBDDDBBB@CCCDDDBBDDEAC?CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17677:1880 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJG0@FGGGEEG@HIGEIGAA9@88/8@?;;65A:3;@@C?@CDD +@HWI-ST575:165:C1P5BACXX:1:1101:18640:1881 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIJGIGJCHIIIJIIJIIIHHFEDDDBDDDD;BDDBBDDDCDDDDDDBCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18890:1897 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIIJJJJJJJIJJIJIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:1102:2244 +CCACGTCTAGCAGGAAGATCGGAAGAGCGTCGT ++ +IHIIHHGIIH@FGDFIIGJHJJGIIGJHGEFD> +@HWI-ST575:165:C1P5BACXX:1:1101:2310:2103 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +BADHGGCCCHHGIHIIH;;44=BAB8;/886855@77<<+>CBDDDDB?:3> +@HWI-ST575:165:C1P5BACXX:1:1101:3289:2146 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTCCCGCGTTCCACTCTCAC ++ +IGGFGGGIEGIIJGHGIGJB:BBB>>88<==?@BDBDBD(>CBDDB@?FCH8AAEB<63;@??B@B8ACDDCC +@HWI-ST575:165:C1P5BACXX:1:1101:6143:2137 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GGD0??)BBB8BBGHFGGHABFB<@?B;<@B=8-AC:?A(4(4>4(++,+:4+4:? +@HWI-ST575:165:C1P5BACXX:1:1101:14357:2064 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJHHJIJJJJJJJJJJJIGHFFDDDDDDDDBDDDDBBDDDDDDDDDDDEDDDDDDDDDCCCCD4(++4+: +@HWI-ST575:165:C1P5BACXX:1:1101:14498:2106 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJIJJJJIJJJJIJJIJJHHFFDDDDDDDDDDDDDDDDDDCDDDDDDDEDDDDDDDDDCDDEE4+++++4 +@HWI-ST575:165:C1P5BACXX:1:1101:14632:2166 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJHIJJJIJJJJIJJJJIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDBDCCDEA4(,+++:4:>AB +@HWI-ST575:165:C1P5BACXX:1:1101:14873:2011 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJGIJJJJJJJJJJJJJJHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDDCCDDE4+,,4+::4@C? +@HWI-ST575:165:C1P5BACXX:1:1101:15054:2152 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJHGICEGGGIJFFIGIIGHFFDDBDBBBDDDDDDBBBCDDDDDDDDDDDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:15855:2031 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCA ++ +IJJIGGIGIIHGIIIGGHGFFFFDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15913:2171 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCT ++ +III:1D?F@<;?=?'0589;8==279@BB8?CDDDDDDDBDECDDCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:16829:2249 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIIHEGGHIGIIIIIGIJIFFFFDBBBDBDD357BA875@;?9>@CCDCDC +@HWI-ST575:165:C1P5BACXX:1:1101:2836:2439 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJJJJJIJJJIIJJJHEEEHFFDDDDDDDDDDDDDDBD9ACDBDDDDDEDDDDDDDDDDDEDD:++44+:44(42 +@HWI-ST575:165:C1P5BACXX:1:1101:4292:2260 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJIIIJJJJJIIIJJJHHFDDD@DBBBD;@DDDBBDDCCDDDDDB?>CDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:4629:2330 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GEHGBDBFGGCAFGEEGBGEB>BC??BB?@B6555:57??@CDB@<@B?:>C?ACDC +@HWI-ST575:165:C1P5BACXX:1:1101:4537:2454 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIHBHGGGIJJJIIJGJIHHFFDDD@=BDB@BDBBDDDCDCDDBDDDDEDDDDDDDBCCCDEE:+,+,+:4+4@? +@HWI-ST575:165:C1P5BACXX:1:1101:5316:2437 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJHIIGIFIJJJGJJJFIEGHGFFCDDDDDDDDDDB7BCCCDDDDDDDCDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5705:2286 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJIJJJIJJJHHFFDDBBD?BB@DDDBBBDCCDBDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6181:2354 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJGIJIJJJJJJJJJJGHHHFFDDBDDDDDBDDDDBBDDDDDDDDDDDEDDDDDADDDCCDDC:++,4+4+,4>? +@HWI-ST575:165:C1P5BACXX:1:1101:6640:2254 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +JJJIJIGIIJJJIIIIIDIHHFDDB@@@?B@?98B@5:BD@CDBDDBDCDCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6520:2397 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJIJJJJJJJJJJJJGHFFDDBBBDDDDDDDDDDDDDDDDDDDBDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7019:2438 +CCGCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTC ++ +JJJJJJIJJJIJIJJIJJHFFFDDDDDDDDDDDDDD:BDCADDDD@4444( +@HWI-ST575:165:C1P5BACXX:1:1101:15331:2257 +CGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJJJJJJGGIHHFDDDDDDDDBDDDDCCD4+++++4 +@HWI-ST575:165:C1P5BACXX:1:1101:16935:2365 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJIIJJJIJGIJIJJGHFEDB@BBBDD6A:9B<=@BD@BB?BDDD8:@CCCDB<5;8ACCCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19565:2447 +CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJFHJJJJJJJJJJIIJJHHFFDDDDDDDDDDDDDDDDDDCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20585:2479 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJJHIJJJIIIJIIJGHIGFHFFCDDDDDDDDDDDB@@DDDCDDDDDDDEDDDDDDCBCCDEEE:4444+:+,+:? +@HWI-ST575:165:C1P5BACXX:1:1101:20888:2259 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +BHIGGHAHG>B9BFHGFHGAE6?A>?BD??D=@BD905@9+37BBBBB?>CAC>@(: +@HWI-ST575:165:C1P5BACXX:1:1101:1310:2696 +CCGTCTGGATCCGCCGAAGCGGA ++ +IJIIGI=FFHHFGGGGGGGEEED +@HWI-ST575:165:C1P5BACXX:1:1101:4969:2645 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GGIGIIJEIGHGHGHIJFGHHFFCBBBDDBDBDDDBBBD?CCDDDDDDCDDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:6485:2694 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJHIJJJIJIJJJJJJJJHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDCDDDDDDCCADDD4+,44(4+44:8 +@HWI-ST575:165:C1P5BACXX:1:1101:6896:2699 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIDHJGGJGHDFHIIG8AEEFE<@BDDDDDB-75<:>55? +@HWI-ST575:165:C1P5BACXX:1:1101:7123:2615 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJIIGJJJJIGHFFDDDDDDDDDDBBD@BDDDDDDDDDD?DDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7199:2618 +CCGATTTGGATCCGCCTAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJIDIIJJJIIHJIJJGGIGHFFDDDDDDDDDDBBDCDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10015:2714 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJGHHIJJJJJJJJJJIJIHHFFDDDDDDDDBDBDDBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10105:2714 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIIGIJJJJJIGJGIJGGFFDDDDDBDDBDDDBHEHFFDDBBB@DDDDDDBBDDCCDDDDDD@DDDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:13944:2746 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAG ++ +IJJIJJIJIIJJJJIJIIIHHFFDDDDDDDDDDDDB@BDCDDD@DDDDDEDDDDDDDDDCCDE@4(++4+ +@HWI-ST575:165:C1P5BACXX:1:1101:14544:2676 +CCGATTTGGATCCGCAGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTG ++ +JJJJJJJJJIJJJIJJJJJIJJHHFDDDDDDDDDDDDDDDDCDDDDDDDEDDDDDDDDDCCDED:(+, +@HWI-ST575:165:C1P5BACXX:1:1101:16211:2706 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +?CG?GBBGGHCHDHIHAAAEHFDCBDBDDDDDBBB@@BACCDDBB@@CDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17293:2515 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJEHIJJJJJIIJJJJJJHHFFDDD;@BDD@DDDB@BDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17341:2572 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +@FG:@D?GFDDBFE??;??@BB/75@>;;8?@@'55875:?CCCDDBDDD?@CCACC@C +@HWI-ST575:165:C1P5BACXX:1:1101:18322:2589 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIJIGJIGEGIIJIIIGGDFDDDDDDDD=:BDBBDDDDCDDDDDDCDDDCDCDC +@HWI-ST575:165:C1P5BACXX:1:1101:18563:2613 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJIJJIJJJIJJHHFFDDDDDDDD8@BD@BDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19030:2538 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DFD)888BF?8??FGBF@G84@<<@>?;,888:@@::98>C@@DDD<ACA +@HWI-ST575:165:C1P5BACXX:1:1101:19375:2502 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIIGGFIJIGHJIIIIJJGEHFFDDDDDDDDDDDDDBBBDCDDDDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19630:2588 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +;FH?F@GHIHBD3CHAFBHGHEFCDBDD?BBDDDDB@@?CDDDDBDDBDEDDDDDDDDDDDEED:4444+:+++48 +@HWI-ST575:165:C1P5BACXX:1:1101:1885:2921 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIJBHIEHGIIIJGJJIHEAHFDCD@>@?BB@BB@BBBD?CCDDDDBB?DCDDCCC@ +@HWI-ST575:165:C1P5BACXX:1:1101:3712:2816 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIIJJJIJIJJJJJJJJIHHFFDDDDDDDD@BBD@B@DDDDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3931:2939 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DFIEH@:CDD;7;@93>C?CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4575:2908 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +EGIHGEEGHHGGEHGGAAGHEEE?BBB8@@BDDDDEDDDDC@A +@HWI-ST575:165:C1P5BACXX:1:1101:4670:2972 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIIJIIJJJJJJJJIJIHGFFDDDDDDDDDDDDDDDDDCCBDDDDDDEDDDDDDDBCCDEEE:4++,,:4+4>8 +@HWI-ST575:165:C1P5BACXX:1:1101:4779:2823 +GATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGTC ++ +JIIIIIJJJJIIJJJIEGIHHFDDDDDDDBDDDDB:@CDDDDDDBDDEDDDDDDDDDDDEEE:++44+4++4>? +@HWI-ST575:165:C1P5BACXX:1:1101:7180:2991 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIFIJIJJJJIJJJJJGIHHFFDDDDDDDDDDDDBBBDDDDDCDDDDDEDDDDDDDDDCADEE:+4:4+4++4>? +@HWI-ST575:165:C1P5BACXX:1:1101:7419:2920 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIIJJIIIJIIJJGIIFHFFDDDDDBDDBDBDBBBDDDDDBDDDDDEDCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7772:2979 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HIHIJJIJIIJJIJJJJJHHHFDDDDDDDDDDDDDD:BDDCDDDD@CC5@;;7B<@@C?C@C> +@HWI-ST575:165:C1P5BACXX:1:1101:12044:2834 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJJJJJJJJJJIJJJJJFHFFDDBDDBDDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12169:2963 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GJJGIIJI*BFIJJJJHIIHHFFDDDDDBDDDDDDBBBDCDDDDDDDBDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:13168:2760 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIFHIJJJJJJJJJJIIIGHFFDDDDDDDDDDDDBDBDCCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13484:2768 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIGHGGIJJGIIIJJJJGJGGFFCBBD?BDDBDDDDDDDCCDDDDD@?CDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13399:2774 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJGIJJJJJJIJJJJIGIHHHFFDDDDDDDDDDDDD:BCCCDBDDDBCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13467:2781 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IHIDHGGIJGIIJJJJIJJIHHFFDDDBDDDBDDDBDDDCDDDDDDD?CECCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13716:2837 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCCGTTCCACTCTCAC ++ +JJJIJJJJIIIJIIIIIJJGHFFDDDBD?BDDDDDDDDDDDDDBBBDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14400:2843 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJIJJJFIIJIIJFIJIGIHHFFDDDDDBDDDD8BB:CDD:+++4+:,+:>@ +@HWI-ST575:165:C1P5BACXX:1:1101:20783:2769 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGAGTTCCACTCTCAC ++ +IJJGIJJIJIJJIGIIIGIHHFFDDDDDDDDDDDDDDBBCCDB@DDBC:ADDD@CDD +@HWI-ST575:165:C1P5BACXX:1:1101:1673:3017 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +?FGDGH0BDFD?B2BBFA +@HWI-ST575:165:C1P5BACXX:1:1101:2153:3048 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAAACTTCG ++ +;D<0@B=DE@'9?(8;'55<5<@@A@:7&5;B?CCDC?A>::2>::>AC:+4+,(4+++:< +@HWI-ST575:165:C1P5BACXX:1:1101:4715:3184 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIJJGGGHI>GGHGGGGEEFFCDDDBBDB/59B9BBDADC@BDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4796:3234 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DHIJJGEHIIIGGCGHEGIHHFFDDB?BBDD@BB@:7@BCDDDBDBBDA@CCCCC:A +@HWI-ST575:165:C1P5BACXX:1:1101:5104:3008 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJIJGIJJJJJIJJGIJJJJHHFFDDDDD@DDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5272:3008 +CCGGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJDEDGHIJIJIFIIJHIEEFD?BDDDBDDDDDDD@@BBDDDDDDDBDD@CDDDCDC +@HWI-ST575:165:C1P5BACXX:1:1101:5594:3119 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +CBFEH=BFAGCB)8;@;;AHFDF>>??=58=2:8557@?3@>@BDBDDD3AC +@HWI-ST575:165:C1P5BACXX:1:1101:6927:3052 +CCGGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DGGEIIGIGGBGF;CGIGGE6BDCCB>BB<@D0CCC +@HWI-ST575:165:C1P5BACXX:1:1101:7098:3130 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJHIJJJJJJJJIJJIIIHHFFDDDDDDDDDDDDDBBDDDDDDDDDDCEDDDDDCDDDCCDEC4(,44+4++4>? +@HWI-ST575:165:C1P5BACXX:1:1101:7842:3018 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JIIIGIGIJGIJIIJJIIIHHFFDDDDBDDDDD@B@@BDDCCBDDDDDCDDDDDDCDDC>>CD>4+,+4+4444:8 +@HWI-ST575:165:C1P5BACXX:1:1101:8316:3056 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJGI?FHEIJJIGJIIIGHFFDDDDDDDDDBBDDDDDDCCDBDDBDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8582:3236 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +EIBB@BBGIIIEHIEBAEAEHFB9;>>8?BDDD8BBD8@CDDDDDDDDDEDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:10343:3076 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJJJIJJJJJHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10433:3184 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGG ++ +JIJIJJIJJJGJIIIJJJJHHFFDDDDDDDD=BDDB +@HWI-ST575:165:C1P5BACXX:1:1101:10787:3078 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIDFHGGEFGGHIJJIIGHHFFDBB>B?@B=@BB<@BBCCDDBDBDBDDCCDCDDC +@HWI-ST575:165:C1P5BACXX:1:1101:10951:3116 +CCGATTTGGATCCGCCGAAACGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJGHIGGIJGGIJICHGGIHHFFFDDDBDDDBBDDDDDDDDCD;BBBDCDDDDDCCD +@HWI-ST575:165:C1P5BACXX:1:1101:11196:3016 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJHGIIJIJJIIGGIAGIGHFFD/;BDBDD@BDDB@BDCCDD@DDD@CDDDCDCCC +@HWI-ST575:165:C1P5BACXX:1:1101:11257:3175 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIIJIJJJJIIJJJIJHHFFDDDDDDDDDDDDBDDDDDDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12061:3148 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJGGGI@FBBGHIJJJJJJGIJGGFFDCA@D:B:BEEFHBEBBABCBBD;BB@<9:B9@CB&5?BCCDCCCCACC +@HWI-ST575:165:C1P5BACXX:1:1101:9555:3271 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIIGEEDDCGGIIBFHEHHHHFDCBD@B?BD?9BB<9@BCCC@BBDBBCCCDADDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9960:3491 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCT ++ +JJJEHIJIJIGGIJJIIJIFHFFDDD@BDDD?BDDDB@BDCA<@BBBBCEDA?> +@HWI-ST575:165:C1P5BACXX:1:1101:11371:3324 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJJJJIJJJJIIJJIJJHHFFDDDDDDDDDDDDDDDDDDDBDDDDDDECDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:11999:3288 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIGHEHJEIHGIIIJJEIIEHFDDDDDBBDDDBBB<@BDCCDDB;BBBDECCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12147:3289 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJIIIGIJJJIHHFFDDDDDDDDDDDDDBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12666:3312 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHJJIJJJJJJJJJJJIGHFFDDDDDBDDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12701:3456 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJIGHJJJJJFJIFHFFDDDDDBBDDDDDDDDDCDDDDDDDDCDDDCDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:13062:3317 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HGIJIIIJEIEGADGHG@GHFDFA@>;@:4+:< +@HWI-ST575:165:C1P5BACXX:1:1101:1525:3749 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +GIIIIJCHIIIIIIJJJIIEHFFDD@DBBDDDDBDBDDD3@A9CDA4,++,+444+42 +@HWI-ST575:165:C1P5BACXX:1:1101:2802:3618 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIFIJJGJJIJJJGJJJJJJIHFDFCCDDBDDDBDDD?CDDBDBDDDDDDDDDDDDCA>CCD4(,+++4:44@< +@HWI-ST575:165:C1P5BACXX:1:1101:3064:3520 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJHIJJFIJJJIJJJIJIHGEFDDDDDDDDDDDDDB7BCDDDDDDBDCEDDDDD@C +@HWI-ST575:165:C1P5BACXX:1:1101:3610:3614 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +IJDHJJFGHIJIEGIJGIIGBFBC@BBBBDD=@BB<@CD?CCC +@HWI-ST575:165:C1P5BACXX:1:1101:4225:3617 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIDGIECBHIDHJIIEHGIFEFFDD@B?BDD?BDBACDED +@HWI-ST575:165:C1P5BACXX:1:1101:4951:3655 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +HIIDHE7DBCDHG@BFHGBHA +@HWI-ST575:165:C1P5BACXX:1:1101:4878:3725 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJIJJJJIJJJHIIHHFFDDDDDDDD?BDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5530:3596 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIGIIEIJIIJJJJIJJIFHFFABDDDDDD@DDDBDBBCDD@BDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6120:3600 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCAC ++ +HIHB>EC@HHBFEBEF@4@6ADB>BBBB8?;?BDD<75??AC575;@8CDD +@HWI-ST575:165:C1P5BACXX:1:1101:6900:3547 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIEGHIJIJJJJJJJJIIGHFFCBBBDDDDDDDDBDDDDDDDDDDDBDDDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7633:3566 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DHEGGE7FCFEGEHGIH8BA:BDA@;BBB=@B@1<:<@FDDHBH84=BDBCAB=@BD?=<8@8@ACCDDDDB?CDDDDADDA +@HWI-ST575:165:C1P5BACXX:1:1101:13315:3747 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +BF5)07@03>C><:AC +@HWI-ST575:165:C1P5BACXX:1:1101:14374:3673 +CCGGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJJGIGJJJJJJIJJIIGGFFDDDDDDDDDDDDDD@BBCDDDDDDDDDEDDDDDDDDD@CCCD4+++++4 +@HWI-ST575:165:C1P5BACXX:1:1101:14502:3661 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JIGDHIGJIGIIIIIIIJIEHFEDDDDBBDDDBDDBCCDBDDDDDEDDDDDDCDD>@CDE:+++4+:44(:< +@HWI-ST575:165:C1P5BACXX:1:1101:2081:3878 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTG ++ +JJIFHIFHIJJJJGIJIJJHHFFDDDDB@DDBBDDBDDD93:9B7BDDDEDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:4430:3860 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HIEHIIDCGGIJIGIIBHIHHFFDDDDDDDDDDDD@<:@CDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6400:3801 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJIJIJJJJJJIIGGHFFDDBDDBDDDDDDBDDDCCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6719:3898 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIIJIGIGIJJJEGGGJIHGFFDDDDDDDDBDDB@BBDDDDDBDDDDDEDDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:8167:3757 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HHGGBG7GCFHIEEGEGB6>BB?AC +@HWI-ST575:165:C1P5BACXX:1:1101:9714:3894 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIIBBDGHGGIIIGGGIJIHHFBCBDBBBDD?<@BB99BCCDBBDDDDCCCDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:11290:3835 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIIIJJIJIJJIJJJJIJHHFFDD@BDDDDDDDDBDDDCDCDDDDDBCEDDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:12038:3902 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IGHGIEGHGGGIIGEHEHIGHFFDDBDDDDDBB8B<5:BCDDDDBDDBCCADDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12700:3779 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJIIIJJGJJHHFFDDDDDBDDDDDDBAAA@=@?8@BD@BDBACCBDDB;BCDCCCCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:13831:3779 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJGGIJIJJHHFFDDDDDDDDDDDDBDBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14731:3871 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIIGIJIJIJIJIIJJHHFFDD@BDDDDDDDDDDDDDDDDDDDDDDECDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14772:3935 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +GAEGGIFHIDHECEFHIEGHHFFCB?DDBDDDD8BBBBD?CCDDDDDBDEDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:15067:3763 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIJGIIIJIGIJIJIIIFIHHFFDDBBDBDDDDDD@@BDCCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:15156:3801 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJIJIJJJJJJIJJGIIIHHFFDDDBDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDCCCDED4(+,++4 +@HWI-ST575:165:C1P5BACXX:1:1101:16559:3892 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +CEF):6BD>B? +@HWI-ST575:165:C1P5BACXX:1:1101:1580:4241 +CCGATTTGAATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJHFGHEHIJJGJIGJJJJHHFFDB?BBDDDB@BBBDDD3:ABBDDDDCDDBDDDDDEDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4437:4204 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTCCACTCTCAC ++ +IIJHIGGIIJJJJIIJGIJHGFFDDDDDDDDBDDDDDBDDCCDBBB@DBDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5319:4088 +CCGATTTGGATCCGCCGAAGGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIJJIJEHFIJIIEGHIJIHHEFFEDDDDDDBBB595?CC>6@BBBBDACCCCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:5702:4094 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJJJJJJJIJJIJJHHFFDDDDDDDD;BDDBBBDCDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5824:4111 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJIIIJIJJIJIJIJIIIJHHFFDDDDD@DDD@DDDACDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6984:4013 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GHBEEDFHGGGDF=EEHGHE4@=>?9;8??@B8BDBDBBDDCDDDDB?CDCDCCDDC +@HWI-ST575:165:C1P5BACXX:1:1101:7890:4076 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FGBHGGGEHGIEIIGIGD@8BECDBD?@BD;=@DDB@?ADDDDDD@?CDDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8052:4159 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +D??GAC@BDB?<==??CBBD?88;805575:?>>CDDBB;??AAC +@HWI-ST575:165:C1P5BACXX:1:1101:8080:4229 +CCGATTTGGATCCGCCGAAA ++ +HDFHEG=BF@DHEHGIJBHH +@HWI-ST575:165:C1P5BACXX:1:1101:8526:4228 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGCGGTTTACCGCGTTCCACTCTCAC ++ +JIJJJJIJJIIJJIJJJBGGGHHEDDDDDDDDDDDDDDDDDDDDDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8765:4049 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGIJJJJIJJIIJIIJGHHFFDB@BBBDD;@BDBDDDCCDDDDDDBCDDDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9822:4042 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJJJIJJJJJGHFFDDDDDDDDDDDDDDBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10184:4066 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIJJJJJJJJJJJJJJHHFFDBBDDDDDBDDDBDDDDDDDDDDDDDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11794:4113 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIJJJJJJJJIJJJJIJHHFFDDDDDDDDDDDDDDDCCCBBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12356:4148 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIIHIIIJICGIIJIGIIIEBFFDB>BB@DD@BBD@BDBCDDDDDDDBDECDCDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:14554:4015 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCA ++ +IJJIGIDHIJIIJIHEIJJHDFFDDDDDBDDBDDDBB<@CCCDDDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:14736:4035 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +IJICCDDDDDDDEDDDDDDDBCDCDDD4++44+:+4+>? +@HWI-ST575:165:C1P5BACXX:1:1101:3262:4389 +CCGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FEB@FEFDGIICEFGBF@BB@3070)529>@CCCCDC +@HWI-ST575:165:C1P5BACXX:1:1101:3996:4500 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGT ++ +JJJIIIJJIJJIJIJJGIGEHHFFCDDDDDDDBDDD:BDCDDDDDD?CDDDDDDDDDCDCEEE:+,,,+4444:29 +@HWI-ST575:165:C1P5BACXX:1:1101:4255:4286 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAA ++ +?D<00)0????B3BDBF;E@8888,3'005BDBDDDD?BDDB<:BDDDDBDDDDDEDCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5886:4450 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +IJBHGGIIGGGGIGEHGGGBHDB?>;;;@CDDD7;2/28?8'07@55<4:<@:A +@HWI-ST575:165:C1P5BACXX:1:1101:9397:4390 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIIIIIGIJIIIJIJJGIJHHFFDDBDDDDDBBBBBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9626:4444 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIIJJJJJJJJJIJJJJJHHFFDDDDDDDDDDDDDDDDDCDDDDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:9920:4375 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJIJJJJJIJJJJJIJJHHFFDDDDDDDD@DDDBDDDDCDDDDDDDDEDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:9928:4484 +CCGATTTGGATCCGCCGAAGCGGATCGTTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GI:@:BFHIEGHIIIGIFIBEDB<@BDCBDDBCBBBBBBCCDDDDDD@CDDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:10347:4290 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJJJJJJIJHHFFDDDDDDDDDDDDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10607:4474 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTATCAC ++ +JIIGGIJJJJJJJIJJIJIHHFFDDBDBDDDBDDDDDBDDDCDDDDBDDEDDCCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11109:4422 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGCGGGGTTTACCGCGTTCCACTCTCAC ++ +CB@6?88D>*9??FEDG@E=;<@;&)07@B5?9A:5C<:ACD +@HWI-ST575:165:C1P5BACXX:1:1101:11719:4366 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJJJJJIIIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11803:4357 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIIIIJIJJJDIJHIJHHFFDDDDDDDDBDDBDDDDCCDD@DDBBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12622:4263 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FGIIJJIIBGIHGIJIEAE6@BBDBBD5550??A@BBBDBBC3:>9CCDD +@HWI-ST575:165:C1P5BACXX:1:1101:13888:4376 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJGIIIHI>GGEHGIHBGEBEFDDBDB@DD@B8B@'=BB'9?8;BBBBDB)5ABD59=;;@BB@5<9:(5<@;B8CC +@HWI-ST575:165:C1P5BACXX:1:1101:21156:4355 +CCGATTGGGATCGGCCGAAGCGGATCGGGCGAGGGGTTTACGGCGTTCCACTC ++ +?1?8@F(?8?);-4'---599><3''38'55077<@@B@?C(57755?(+4>9 +@HWI-ST575:165:C1P5BACXX:1:1101:21005:4492 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GFFGIICIIIHIEHGIDHEEEFDA@DDDDD=@DDD57?CC:97BDB?CDCDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:2208:4721 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJGHIJGIJJJJJJJIIJHHFFDDBDDDDDBDDBBDDD>ACB@BDDDDEDDDDDDDDDCCDDD:++(,+:34+>8 +@HWI-ST575:165:C1P5BACXX:1:1101:3594:4541 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IGHHIJIJJJJFBHHIEIEHHFDDDDDDDD=@DDDD9BDDDDDDBBBDEDDDCDDC +@HWI-ST575:165:C1P5BACXX:1:1101:4453:4680 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIIIIJIIJJJJIJJIJDHHFDDDDDDDDDBDDBBBBDDDCDDDDDDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4574:4514 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC ++ +JJJIIJIJJJJIJGHJIJIHHFFDDDDDDDD;BBDBBDDDDDDDDDDDDEDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4512:4554 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IIJIJJFIJJJJJIJIIIGHHFFDDDDDDDD?DDDDDBDCDCBBDDDDDECDDDDDDDDDCDDE:44+,+:4+4>? +@HWI-ST575:165:C1P5BACXX:1:1101:6091:4740 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +CFF0:BDGG38?BBCBF8328;=;@B@9@<9?*).3,8,823)-77B5??>ABDDD;(9 +@HWI-ST575:165:C1P5BACXX:1:1101:14064:4581 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAG ++ +IJJIJJIJJIJJJGIJIGGFHFFDDBDDDDDDDDDBDBBCDDDDDDDDCDDDDDDDDDCCCEED:+++4+ +@HWI-ST575:165:C1P5BACXX:1:1101:14735:4724 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIGJIJJIJFIGIIJJGEHFFDDDDD1;B;@BDB@CBDDDDDDEDCDCCDD +@HWI-ST575:165:C1P5BACXX:1:1101:18593:4710 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIIHEHCHIJJIJIGGGEEFFDDBDB?BB/7A8@???FFEDHEG<>C9>:@C +@HWI-ST575:165:C1P5BACXX:1:1101:16043:4995 +CCGTTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCCTCTCAC ++ +IJJHIJJJJJJJJIJIIJJGGFFDDDDDDDDDDDDBEFF>BDB?BB?8;@B578>>CDDB<;8<3:>GIIIJJJHHFFDDDDDD?BDDDDDDDDDDBBDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18453:4916 +CCGATTTGGATCCGCAGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +EGG:FEGHHHGI;FH? +@HWI-ST575:165:C1P5BACXX:1:1101:19870:4937 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJHGIJJJIIGHIEHIIIGHFFCDBDD@BDBBDDD7@BCDCBDDDDD8 +@HWI-ST575:165:C1P5BACXX:1:1101:19796:4989 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJJJJJJJJIJJIJJJJIHHFFDDDDDDDDDDDDDDDDDDDDDDDDDDEDDDDDDDDCCDEEE:+,+4+:4+:>8 +@HWI-ST575:165:C1P5BACXX:1:1101:20053:4972 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC ++ +IIDDHGIJHGGIGHIIDIHHFF>ABDDDDD?DDDDBBFGGIH?B@ABGE3=>?@@BBBDBD8?33>:&57@8ADDC<@@CC?AADD(::4>:44:+:4:? +@HWI-ST575:165:C1P5BACXX:1:1101:1686:5115 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJJJJJHIGHHIJJJGJIHHFF@B@BBDDDBDDDBBDD9@CBDDDDDCDCCDDDACB@ACEDD:+444,:>>>4< +@HWI-ST575:165:C1P5BACXX:1:1101:2938:5127 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HIEDFHIG< +@HWI-ST575:165:C1P5BACXX:1:1101:8840:5173 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGIJJJJGIJJJJJJIJFBEFDDBDDDDD=BDDBBDDDDDDDDDCDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9731:5160 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJDFHJGIIIIJJIJJIJJGHFFDDDDDBDD;BDDDDDDCDCDDDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:10830:5146 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IGHIIJIIJJIFEEIJBHABHFDDD??DDDBD@@BD:BDCCDDDBB?CDCCCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11032:5068 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJIJJIIJJIJJIJJJGGFFDDDDDDDD;BBDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11565:5085 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIEHHIJJHIJJJJJJIIFFFFDDBDDBDD?BBD<<@BDDDDDDDD?CEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12209:5017 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC ++ +?D)00B0BDF>*?CGB@5AA<9638'9=8,2257855:@C:>@50552<3>:9 +@HWI-ST575:165:C1P5BACXX:1:1101:12070:5238 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJJJJJJJJJJJJIHGFFDDDDD?BDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12614:5234 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJJJJJIJIJJJJJHGFFDDBDDDDDDDDDDDBBDDCDBDDDDDEDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:12934:5066 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJIJJJJJJJJJJJJJGEFFFDDDDDBDDDDDDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12889:5153 +CCGTCTTGGATCCGCCGGAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +JJGHIJJGIIJJJGHIGIHFFFDDDDDDBBD1BDD@57BCDCD@DDDBDECDDCDC +@HWI-ST575:165:C1P5BACXX:1:1101:13006:5008 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +:C????;F98??B=1;AEG4-=5=>/,'(,,,))57@5??CCDDDD@2?C@C +@HWI-ST575:165:C1P5BACXX:1:1101:13701:5054 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJJJJJJIIJJJJJJJGHHFFDDDDDDDDDDDDDDDDDCDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13818:5190 +CGATTTGGATCCGCCAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJHIJJJJJJJJGIJJJHIJJJJJHHHFDDDDDD=8?CDCDDDBDDCCDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14721:5211 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC ++ +DHBHIF@GHIGEGGIBHG6EHEDFDDDDB@;?BDDBCCC:@@CD +@HWI-ST575:165:C1P5BACXX:1:1101:15180:5074 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +)CGHIJIJJIJJJIIIGGHHHFFDDDDDDDDDDDDDDDDCDDDDDDDDDEDDDDDDDDDDCEEE4+++++::4>C@ +@HWI-ST575:165:C1P5BACXX:1:1101:15733:5028 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJHIJJJJJJJJIJJJJJHGFFDDDDDDDDDDDDDBDDDDDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:16783:5148 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIIHJJIJJJIJIIJJIJIGHFFDDDDDDDD?BDDB@BBDDDBB@DBDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:17093:5065 +CCTATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJJJJJJJIGIHHFFDDDDDD@DDDDBBDDDDDBDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18177:5052 +CCGCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJJIIGIFGGJIGHA/@BEBD?>BDDDDDDDDDD05?CDDDBD;7GIGHBBBHEBABBBD@BDDDDDBBBBDDDDDDDDDDEDDDDDCC +@HWI-ST575:165:C1P5BACXX:1:1101:21070:5172 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +?F<086BFHE8?B,8=BDDDB758>CABD??@8:4+4444>:4+ +@HWI-ST575:165:C1P5BACXX:1:1101:2332:5476 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIJJJJIJJIJJJJEHHHFFDDDDBDDDDDDDBBBDACCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3549:5422 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIJJJIIJJJJIJJGIJHHFFDDBDDDDDDDDDBDDDCDCDDBDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:3934:5255 +CCGATCTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJJGG6DHIJJJIIJJIJFHFFDDDDDDDDD@BDDDDBDDDDDDDDDDDEDDDDDDDDDCCEED:44+,+:++4>? +@HWI-ST575:165:C1P5BACXX:1:1101:3779:5257 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJIIJJJIJJIIJJJJIJIHHFFDDDDDDDDBDDD@BBDCCCDBDDDDDDDDDDDDDDDDDDDE::44,+:444@? +@HWI-ST575:165:C1P5BACXX:1:1101:3981:5396 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +CGD@FAFIIIEGEGEBFGGBGIDFEDDC=>?>BBD<95?ACCDDDDBBDECDCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4352:5435 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJIJJJJJJJJIIJHGFFDDDDDDDDDDDDDDBDDDDDDD;@DDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5757:5381 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +CFG>BGBFGGHD@BFFHGGEEED?8;?528@95B7<529ACBDDDD?CD:A<@@CC +@HWI-ST575:165:C1P5BACXX:1:1101:1536:5742 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HJIEIJIGGBHGICIIBHIGHFFDBBDD?BDB9BDB5CCDDDDDDDEDDDDDDDDC@CDEE:++4,+44+(:2 +@HWI-ST575:165:C1P5BACXX:1:1101:4347:5721 +CAGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIHGIGAAEHIIIEDHEHJIJHFFFDDDDDD:@DBB77BCCCBDDDDDCDCDCCDDC +@HWI-ST575:165:C1P5BACXX:1:1101:5268:5661 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +CF:)8)8DDB;;88;8'00:7@889AA5@BDD@DECC?CDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6568:5658 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIJJJJJJJIGJJGGGHGFFDDDDDDDDDDBDBBBDDDCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7344:5519 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIGHIJJJIJJJJIIJGGHFHFFDDDDDDDDDDDDBBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8216:5553 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJEIJJJJJIJJIJJJGGEHFFDDDDDDDDDDDDDDDDDDDDBDDDDCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:8183:5554 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC ++ +?DFHI;?BC<:ACD +@HWI-ST575:165:C1P5BACXX:1:1101:12148:5538 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJEIJJIJJJJJJJJJIIFGFFDDDDDDDDDBBDDD@BDBBBDCDCDDCCDC +@HWI-ST575:165:C1P5BACXX:1:1101:13615:5616 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIJJJIGJIJJIJJJIHHFFDDBBBDDDDDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13537:5735 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJIJIIIJJJIGIIGGHFDDBDDDDDDDDDBBBDCDDDDDDDDDEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:14584:5542 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JJJIJJJJJJJJJJJJJJJHHFFDDDDBDDBDDDDDDDDDDDDDDDDDCDDDDDDDDDDCCDEE4(,+++4 +@HWI-ST575:165:C1P5BACXX:1:1101:15724:5746 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACC ++ +IJJIJJJIJJJJJJJJJJJHHFFDDDDDDDDDDDDDB@DDCDDDDDDDDEDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:17227:5625 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTC ++ +FGIB?B*?3?BBFBFG6A8;:EB<9A?=;;;289@&0893@BD;5;(+:>C9>@ +@HWI-ST575:165:C1P5BACXX:1:1101:17881:5507 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJIJJJJJJJJJJJHHFFDDDDDDDD8@DDBDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20112:5554 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +>GI:D;DHFG@B8BF;AEHEEDD>@>>B5=BDDDD@B@<>CCDDDDDBDECCCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20322:5548 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +BHI06??DGE/;3>B?@B=BB8700CC<(+: +@HWI-ST575:165:C1P5BACXX:1:1101:21233:5679 +CCGATTTGGAGCCGCCGAAGCGGATCGGGCGAGGGGTTTACCGCGTTCCA ++ +1?D)0)0?99)9(7@'5':<<>6'9'88'27BB8B970(+>37BDBD?CD +@HWI-ST575:165:C1P5BACXX:1:1101:1959:5920 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJHGJIJIJJJJFIJJGIFHFFDDDDDDDDBDBDDDDD?CCDDDD;@CDDDDCDDDDDCCDED:44(4+4,4+>8 +@HWI-ST575:165:C1P5BACXX:1:1101:1924:5978 +CCGATTTGGATCCGCCGAGGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJJIIIGJJIGHIJIGJGHGFDDDDBDDBD85:BDBDDBACDDDDDDDCDCDDDDDDDDCCEDE4+,44+4+++:8 +@HWI-ST575:165:C1P5BACXX:1:1101:4153:5778 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIJGIGGIJJJJIJJIGIIHHFFDDDDDDDDDDDD@<@C +@HWI-ST575:165:C1P5BACXX:1:1101:6188:5778 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHJJJJJJJJJJJJJJJHHFFDDDDDDDDDDDD@BBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:6867:5946 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGTTCCACTCTCACCATTTCTAGTGAGACTTCGTC ++ +JJJIJJJJJIJJIIJJIGIHHFFDDDDDDDDDDDDDDBDDDCDDDDDEDDDDDDDDDDDEEE@44>44:+,4>?CB +@HWI-ST575:165:C1P5BACXX:1:1101:7535:5814 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIGIJIJJJJJJIJJJGGHGFFDDDDDDDDDDDDDB7@CDDDBDDBBCDCDDDCAC +@HWI-ST575:165:C1P5BACXX:1:1101:7794:5903 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIIGGIJJJJIIJHIJHHFFDD@DDDDBDDDDDDDDDCDDDDDDBDDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9106:5832 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIIIJJJJJJJJIJIIEEFFDB@@BBDD;:BEDC@DDB?BB-95<05<9:CDD75<ACCCCC +@HWI-ST575:165:C1P5BACXX:1:1101:9549:5793 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +DG>@FG6BFAGGICGHGGIBEEDCD?>8@??;@@BBBBBCDDDDDDBBCDCDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10541:5867 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJFHJJJJIIJJJJJJJJHHFFDDDDDDDDDDDDBBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:11397:5818 +CGATTTGGATCCGCCGAAGCG ++ +H?)@DGI@DD4+++++4 +@HWI-ST575:165:C1P5BACXX:1:1101:14716:5991 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JIJGIJJJJJJJIEHJIJIHHFFDDDDDDDDDDDDDB@DDDCDDDDDDDDDDDDDDDDDDDDDE:,++4+:44>A@ +@HWI-ST575:165:C1P5BACXX:1:1101:15153:5829 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGAGTTCCACTCTCACCATTTCTAGTGAGA ++ +DHIHIGGIJIJJGIJIJGGFHFFDDDBDDDDBDBDBDCADD4+++++4 +@HWI-ST575:165:C1P5BACXX:1:1101:15618:5813 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +HGGGGHIJIEGBFFEGHDGHHFFDBBBB@==89@@<07@>@CBDDDBD?DCDDDDA +@HWI-ST575:165:C1P5BACXX:1:1101:16278:5962 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAAGGGTTTACCGCGTTCCACTCTCA ++ +GHIABFHDGGIG>FGIGFHFDFDBD?BDDDD8BBD@BABC:AB;BDDDCDDDDCDA +@HWI-ST575:165:C1P5BACXX:1:1101:17404:5768 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIFDGHIJJJJJJJJJIJIEHFFDD@BDDDD;@DDBBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18508:5770 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIGIJJJJJJJJIJJIJIHHFFDDDDDDDDBDDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18654:5893 +CCGATGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGTC ++ +IJJJJJJIJJIIJJJFHCHIIGHIEHFFDDDDB@DBDDDDDDDBDDDDDDDDDCDDDDCCDDDCC@ADD:(++,+4++4:2 +@HWI-ST575:165:C1P5BACXX:1:1101:20461:5768 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +IJJDFFHGIHIIJIGGIJJHFEDCD>BD@BBB@DBBBBDDCDDDDD@?AA@CCCA> +@HWI-ST575:165:C1P5BACXX:1:1101:1693:6048 +CCGATTTGGATCCGCCGAAGCGGGTCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IGGGHGGGHEGHGHGIIDEGAHFBCDDD@@D9:B8::B?3:ABB<@DD?:CC<@@CCDCCCACC444:4+:,44>8 +@HWI-ST575:165:C1P5BACXX:1:1101:3291:6004 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIIJIIJJJJJIJJGHIHHFFDDDDDDDDDDDDDDBD?CDDDDDDDDEDDDDDDDDDCCEEE:4444(44++>8 +@HWI-ST575:165:C1P5BACXX:1:1101:3937:6198 +CCGATTTGGATCCGCCGGAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIHIJJJIIIJJJJJIJHHFDDDDDD'8@DBBDDDDDDDDDDDDDDDDCDDDDDCC +@HWI-ST575:165:C1P5BACXX:1:1101:4273:6038 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIGIIIIIIJIGIIIGFHFFCDDDDDDB:@BDBBDDC>ABBDDBDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:4445:6115 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +HGFC>CA +@HWI-ST575:165:C1P5BACXX:1:1101:4344:6153 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIJJJJJJJJIJJJGBHFGFFDDDDDDDD@DDDDDDDACDDDDDDDDEDDDDDDDDCCCDEE4+++,+4444@? +@HWI-ST575:165:C1P5BACXX:1:1101:5212:6077 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIJIIIDCGGHIJBHHIIGEH@BBDDDD;B@DDBDBCCCDBDDBDCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:5854:6193 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACT ++ +JJIIJJJJJEHIJJJJJJJHHFFDB@BBDDD:@BDBDBBCDDDDDD;BDDCDDDDDDDDCCDEE>4444+4+4 +@HWI-ST575:165:C1P5BACXX:1:1101:6093:6246 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +JJIEIJGHIIIJFJJGGBGEHFB>B>B@BDD25BCDHIFEHHIIEA6AAEE6<>@B@??@/7CDD +@HWI-ST575:165:C1P5BACXX:1:1101:8253:6223 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGGTTACCGCGTTCCACTCTCAC ++ +DGFH=DD?3BFDCCDA;'@8@CD +@HWI-ST575:165:C1P5BACXX:1:1101:8552:6031 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +FE0@FGCGBGEHIIGJGABEHFCDDBDDDDDBDBD578ACABFFFEAGCDDB5<<<4@CACCCD +@HWI-ST575:165:C1P5BACXX:1:1101:9569:6015 +CGGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJIJJJJJFIJJJJJJJHGHFDDDDDDD/=5:BBBBBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10905:6024 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +CCD@?@8D@*9?DHDBG@?CC +@HWI-ST575:165:C1P5BACXX:1:1101:11477:6010 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +JIIGIJIJJIIIJJJIIIIEHFFDDBB@?BDBBDDDDBBDCDDBBBD?ADDD +@HWI-ST575:165:C1P5BACXX:1:1101:12389:6154 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +JJJIJJJJJJJJJJJJIGIEHFFDDDDDDDDBDDDBDDBDDDDDDDCDDEDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12820:6019 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GGHE>HGHGEGGIJE;FHI>6BDDD@BDDDDBB@DDB8?CCD57@@B@DD:A9A:@C +@HWI-ST575:165:C1P5BACXX:1:1101:13503:6047 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJHIJJIJJJIIIIJJGIGHGIHFFDDDDDDDDDDD:BDDCDBDDBBCEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:14071:6041 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JGB@FHIHIEI@BFEDHGIHHFFDDDD=B?B@BBBGIGGFFDBDDDDDDBDDDB:BCCCDBDDB?ACDDCDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:17938:6081 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGJJJJJFIJIIJJIJJHHFFDDDDDDDD=BDDDDBDDDDDDDDDBDDCDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:19315:6150 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +B8CF)?69B@B*9?BFGB8@AADFABDB?;@=?B<5)5?3:AB@75;BCDCDACCDC +@HWI-ST575:165:C1P5BACXX:1:1101:19532:6018 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIIIJJJJJIGIGHIIJEFGFFDDDDDDDDDDDDB5CDBB@@D7CDCDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:1089:6262 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIBFHGIJIGHHIHIJGHBCA4(::4(4:8 +@HWI-ST575:165:C1P5BACXX:1:1101:1829:6451 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGATTTACCGCGTTCCACTCTCAC ++ +JJJIJJIJJEIIJEHIEGIHHFFCDBDDBDDDBDDDACCDDDDDDDDDADDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:2484:6444 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +G?@DFC=FG*9?D9BFHGHABBB83<@:=B-9@BACBDDDB8?DCCA@ACC<@>@@DC:44+,++(,:>? +@HWI-ST575:165:C1P5BACXX:1:1101:3874:6280 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJJIJJJIJJJIJIIIIJIEHFFDDDDDDDDBDDDDDDDACDDDDDDDDDDDDDDCDBCCDDDE:+44,+:44:C@ +@HWI-ST575:165:C1P5BACXX:1:1101:4404:6415 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIHIJJIIIJJJJJJIIIFHFFDDBDDDDDBDDDDDDDDDDDDDDDDDEDDDDDDDDDDDEED:++4,(44+4:< +@HWI-ST575:165:C1P5BACXX:1:1101:5416:6451 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +H@GD@?;FDBFHHIBHGG>EB?DCDDDDDDDDDDB<57@>:>@<@@@BCCDCCDCCDBC:::CC4+++,+:4+4>8 +@HWI-ST575:165:C1P5BACXX:1:1101:7066:6455 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJFJJIJJJJIJIJIHHFFDDDDDDDD?BDDDDDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7488:6251 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJGJJJJJJJJJIJJIIIHHFFDDBDDDDDDDDDBBDDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7486:6329 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIJJIJJIJJJIJJJGHHFFFDDDDDDBDBDDDDB7?CCDDDDBB??ACCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:7643:6450 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGT ++ +JEDFHJIHICIGIGIJGJJHHFDAB>?=??==@BBBB@DBB +@HWI-ST575:165:C1P5BACXX:1:1101:8454:6487 +CCGTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIGHJIJICHGHHIJJGBGFFFDBBDDDDB?BDBD55?C>CBDD@B8<>ACCDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9137:6329 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIFIJIJJIJJJIJJJIIHFFFDBBDBDDDBDDDDDBDDCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9587:6277 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTATCGCGTTCCACTCTCAC ++ +IIBGEIIJJIJJJIJJJGHEHFFDD@DDDDDBDDD<7ADDD4(++,+444:@( +@HWI-ST575:165:C1P5BACXX:1:1101:10054:6289 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGTTCCACTCTCAC ++ +JJJGIJJIJJIJIIJJGHGHHFFDDBDBBBDDDDDDB@BCCCDBBCCCCCDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:10173:6294 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGGGCCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCGTC ++ +JJIGEIJIJJJJJJJJIJJHHFFDDDDDDDDBDDDDDDDDDDDDDDDDCACDDDDDDDDEEE@::4,+4++4:?C@ +@HWI-ST575:165:C1P5BACXX:1:1101:10764:6333 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +HIJIJIGICGGIIEHIABEGHHDEC?>>;?B8=85757@ACDDBDDBDCCC@ +@HWI-ST575:165:C1P5BACXX:1:1101:11047:6477 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACT ++ +GDGDGEGGFFBB?CDEB;EEEEF<>;?@5;?8<58<:@B>C>@;BBDBACCC +@HWI-ST575:165:C1P5BACXX:1:1101:11411:6372 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTC ++ +JIJIJIDIHIJJJFGHBGGHHFFDDBB=?BB?BDB@8@BCDDDDDDDDCDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:11665:6448 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJIIJJJIIJJIJJGIIHHFFCDBDDDDDDDDDBBDDDCDDDDDDB?DDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12774:6457 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +GIBDHIGGIJIIJEIF@@EAEFBBDB@?@@?;8BDBDB?CC>9B7@@BBB@@@BB00??>@507;<<<>CCDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:18173:6282 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJIHJJIJJIGIJIJIIIJHHFFDDDDDDDDDDDDDBDDDCDDBBDDBCEDDDDDDC +@HWI-ST575:165:C1P5BACXX:1:1101:18209:6492 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJHIGIJJHJJJJJJJJIFHFFDDBDDBDDBDDDDDDDDCDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18546:6430 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJHIJJJJJJJJJJJJIGFFFDDDDDDDDDDDDD<@DDCDDBDDDDBDEDDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:19023:6315 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIIJJIIIGFHJJJIJBHEHGHFFDDD@BDBDDDD57@CCDDDD<@8@CD;BDDDDECDDCCDDBCDADAC::434+:4:::? +@HWI-ST575:165:C1P5BACXX:1:1101:20043:6415 +CCGTCTGTGTCCGAGATCGGAAGATCGTCGT ++ +FHGGG@F0??4?F(<:@FGG@DHCEHH3;B> +@HWI-ST575:165:C1P5BACXX:1:1101:1982:6500 +CCGGTGTCCAGATCGGAAGAGAGTCGT ++ +JFGE8@?FHGGCBCEIIGIDHHIHEEH +@HWI-ST575:165:C1P5BACXX:1:1101:1934:6651 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +JJIGIJJJJIEIJGIJIJIHHFFDDDDDDDDDDDDDDDDDCDD@BDDDDEDDDDDCCBDDDEEE:44:4+:4+4>0 +@HWI-ST575:165:C1P5BACXX:1:1101:3031:6587 +GATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGA ++ +JHIIJIJJIJJGIIGHHFGIIHFFFDDDD@DDDD<:?CDDDDDDDDDDDDDDDDDDDDDEEE>++:44: +@HWI-ST575:165:C1P5BACXX:1:1101:3659:6632 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACT ++ +JJJHIJIIJHGIJJJJEHJHHFFA@BBB@BD@DDD@D0078344 +@HWI-ST575:165:C1P5BACXX:1:1101:6970:6733 +CCGATTTGGATCCGCCGAAGCGGATCGGTCAAGGGGTTTACCGCGTTCCACTCTCAC ++ +IIFEGHIIGGGEHGEGHGHGEHDFC>@>@BB>CCDBDBDDDDDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:9786:6627 +CCGATTTGGATCCGCCGAAGCGGATCAGTCGAGGGGTTTACCGCGTTCCACTCTAAC ++ +JJJIJJJJJJIJJJJJJIJFHFFDDDDDDDDBDDDDDBDDDDDDDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:10466:6616 +CCGTCTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJEHJJJJJIJIJJJJGGHBEBFCC@DBBDDBDDDD9@DCDDDDDDDBDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:12094:6626 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIIJJJJJJJJJJJIIIHFFFDDDDDDDDDDDDDDDDDDDDDDDBDDEDDDCDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13236:6588 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +IJJHJJJJJJJJJIJJIJJHHFFDDDDDDDDDDDDBDDDDDCDDDDDDDEDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:13123:6721 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCCGTTCCACTCTCAC ++ +JJGFHGIIJJGIJIIJIIJFHFFCDDDDDBDBBDBBBBBDDBBD=BDD@5<@?:@B@;>@>HII@GIIGJFGGGIJH8=DDBB@BBDDBDDDB07?>ACDBBD@BCDDDDDDCD +@HWI-ST575:165:C1P5BACXX:1:1101:20819:6735 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAGACTTCG ++ +IJJIJIJJJEEIGIJGGGHHHFFDDDDDBDDDDDDDDBDACDDBBDDDCDDDCDDDCBCC@DDE:+,+4+:+4:@? +@HWI-ST575:165:C1P5BACXX:1:1101:1564:6811 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HIBEDH;DCFHHIIJIJGGBGGAED +@HWI-ST575:165:C1P5BACXX:1:1101:1927:6913 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJE@BBDD8<< +@HWI-ST575:165:C1P5BACXX:1:1101:6911:6752 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJJJJJJJJJJJIJJJIJHHFFDDBDDDDDBDDDBDDDDDDDDDDDDDEDDDDCDD +@HWI-ST575:165:C1P5BACXX:1:1101:8402:6992 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +HGE?FD;GBCDDDD?8@87@??BBBDD@75@@ACCDBBBBBA:AACCA +@HWI-ST575:165:C1P5BACXX:1:1101:17036:6768 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JIIIGGGIGEIIJJDIIFIEEFEDCBDDDDDDDDD@BDDCDCDDDDDDCEDD?CCDD +@HWI-ST575:165:C1P5BACXX:1:1101:17542:6830 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJIIIJJJIIHHFFDDBDDDDDDDDDDBDDCCCDBDDDBCDDDDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:18093:6840 +CCGATTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCA ++ +GIIIGGIJ3DHDH=FE/@EEDFAA8?BDBBDDDB@)5?9:>@5(88;;-750023>307;@B?C@CA +@HWI-ST575:165:C1P5BACXX:1:1101:19230:6988 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJJJIJIJJIHGFGFFDDDDD@BDDDDDDBDDCDDDB@BDDDCCCDDDDD +@HWI-ST575:165:C1P5BACXX:1:1101:20486:6800 +CCGCTTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCACCATTTCTAGTGAG ++ +JJJJIJJJJJJJJIJJIJIHHFFDDDDBBDDDDDDDBDD?CDDBDDDDDEDDDDDDDDDD@DDD:++44+ +@HWI-ST575:165:C1P5BACXX:1:1101:20297:6938 +CGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JID@GIJJHGHGGIGI;ABEFBBDDDDDBDBDDDD@5@DDDDDDDDBDCAC:4>:4:@8 +@HWI-ST575:165:C1P5BACXX:1:1101:1500:7064 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJJIJJJJJIGIJIIJJJJHHFFDDBDDBDDDDDDBDDDDDDDDDDDBDEDDDDCCC +@HWI-ST575:165:C1P5BACXX:1:1101:1577:7179 +CCGATTTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJDEGHHJJJJI@HIIGEGH:B?88;=;9BD@:<@CDCD;A9@(4@ +@HWI-ST575:165:C1P5BACXX:1:1101:2297:7148 +CCGTCTGGATCCGCCGAAGCGGATCGGTCGAGGGGTTTACCGCGTTCCACTCTCAC ++ +JJIGIJIJIHIJIJIGGIBEADBCBDDDDDDBDDD97@CCD@DB;;?CCCDDDDDD diff -r fb76303acf4f -r 431aebd93843 test-data/trimming_stats.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/trimming_stats.txt Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,1 @@ +No trimming: 534 , out of which not recognized MD field for: 0 ; 1 nt trimmed: 90 ; 2 nt trimmed: 14 ; 3 nt trimmed: 9 diff -r fb76303acf4f -r 431aebd93843 test-data/unique_barcodes.txt --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/test-data/unique_barcodes.txt Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,108 @@ +RNase_P 110 187 2 +RNase_P 111 187 4 +RNase_P 112 187 2 +RNase_P 115 187 1 +RNase_P 117 187 5 +RNase_P 117 188 1 +RNase_P 119 186 1 +RNase_P 134 188 1 +RNase_P 137 187 1 +RNase_P 138 187 1 +RNase_P 152 187 1 +RNase_P 10 187 1 +RNase_P 11 187 1 +RNase_P 15 187 2 +RNase_P 30 187 2 +RNase_P 31 186 1 +RNase_P 31 188 1 +RNase_P 18 186 1 +RNase_P 18 187 1 +RNase_P 32 187 1 +RNase_P 19 187 1 +RNase_P 33 187 1 +RNase_P 50 187 2 +RNase_P 51 187 1 +RNase_P 38 187 1 +RNase_P 39 187 1 +RNase_P 55 187 3 +RNase_P 56 187 1 +RNase_P 2 187 2 +RNase_P 57 187 2 +RNase_P 3 185 1 +RNase_P 3 186 9 +RNase_P 3 187 94 +RNase_P 3 188 1 +RNase_P 58 187 3 +RNase_P 71 187 2 +RNase_P 59 187 1 +RNase_P 4 185 1 +RNase_P 4 186 32 +RNase_P 4 187 289 +RNase_P 73 187 1 +RNase_P 5 187 14 +RNase_P 6 186 2 +RNase_P 6 187 10 +RNase_P 7 187 4 +RNase_P 8 187 4 +RNase_P 9 187 3 +RNase_P 77 187 4 +RNase_P 90 187 2 +RNase_P 78 187 2 +RNase_P 92 187 2 +RNase_P 93 187 1 +RNase_P 94 187 2 +RNase_P 97 186 1 +RNase_P 97 187 4 +RNase_P 100 187 1 +RNase_P 101 187 2 +RNase_P 98 187 1 +RNase_P 99 187 5 +RNase_P 102 187 1 +RNase_P 103 187 1 +RNase_P 104 187 1 +RNase_P 105 187 1 +RNase_P 106 187 2 +RNase_P 107 186 1 +RNase_P 107 187 3 +RNase_P 121 187 2 +RNase_P 109 187 2 +RNase_P 125 187 1 +RNase_P 126 187 2 +RNase_P 128 187 3 +RNase_P 129 187 1 +RNase_P 142 187 1 +RNase_P 145 187 1 +RNase_P 147 187 1 +RNase_P 148 187 1 +RNase_P 163 187 1 +RNase_P 169 187 1 +RNase_P 20 187 1 +RNase_P 21 186 2 +RNase_P 21 187 7 +RNase_P 22 187 2 +RNase_P 23 185 1 +RNase_P 23 187 4 +RNase_P 24 187 1 +RNase_P 25 187 1 +RNase_P 27 186 1 +RNase_P 27 187 1 +RNase_P 40 186 1 +RNase_P 40 187 7 +RNase_P 41 187 1 +RNase_P 42 187 1 +RNase_P 43 187 1 +RNase_P 44 186 1 +RNase_P 44 187 1 +RNase_P 45 187 2 +RNase_P 48 186 1 +RNase_P 63 187 1 +RNase_P 64 187 1 +RNase_P 65 187 1 +RNase_P 81 187 2 +RNase_P 68 187 1 +RNase_P 82 187 2 +RNase_P 83 187 1 +RNase_P 85 187 2 +RNase_P 86 187 1 +RNase_P 89 147 1 +RNase_P 89 187 6 diff -r fb76303acf4f -r 431aebd93843 tool_dependencies.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/tool_dependencies.xml Wed Aug 05 09:21:02 2015 -0400 @@ -0,0 +1,18 @@ + + + + + + + + + + + + + + + + $REPOSITORY_INSTALL_DIR + +