# HG changeset patch # User nick # Date 1450726438 18000 # Node ID 197fa659cdfa4141ca3e140a31e3b7e9e8ab6d24 # Parent ba81823ad9e1fe3e08d650c14e12d6eb4d07332e planemo upload commit 7a0fc15e2741268c2d5db6b5fa7cde5af057401e diff -r ba81823ad9e1 -r 197fa659cdfa align_families.xml --- a/align_families.xml Tue Dec 08 08:57:34 2015 -0500 +++ b/align_families.xml Mon Dec 21 14:33:58 2015 -0500 @@ -1,10 +1,11 @@ - + of duplex sequencing reads mafft - duplex + duplex DUPLEX_DIR + python \$DUPLEX_DIR/align_families.py -p \${GALAXY_SLOTS:-1} $input > $output diff -r ba81823ad9e1 -r 197fa659cdfa dunovo.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/dunovo.xml Mon Dec 21 14:33:58 2015 -0500 @@ -0,0 +1,62 @@ + + + from duplex sequencing alignments + + duplex + DUPLEX_DIR + + + duplex.fa + && awk -f \$DUPLEX_DIR/utils/outconv.awk -v target=1 duplex.fa > $output1 + && awk -f \$DUPLEX_DIR/utils/outconv.awk -v target=2 duplex.fa > $output2 + ]]> + + + + + + + + + + + + + + + + keep_sscs + + + + + + + + + + + +**What it does** + +This is for processing duplex sequencing data. It creates single-strand and duplex consensus reads from aligned read families. + +----- + +**Input** + +This expects the output format of the "Align families" tool. + +----- + +**Output** + +This will output final, duplex consensus reads in two FASTA files (first and second reads in the pairs). Optionally, you can save the single-strand reads too, in a separate FASTA file. + + + diff -r ba81823ad9e1 -r 197fa659cdfa duplex.xml --- a/duplex.xml Tue Dec 08 08:57:34 2015 -0500 +++ /dev/null Thu Jan 01 00:00:00 1970 +0000 @@ -1,61 +0,0 @@ - - - from duplex sequencing alignments - - duplex - DUPLEX_DIR - - duplex.fa - && awk -f \$DUPLEX_DIR/utils/outconv.awk -v target=1 duplex.fa > $output1 - && awk -f \$DUPLEX_DIR/utils/outconv.awk -v target=2 duplex.fa > $output2 - ]]> - - - - - - - - - - - - - - - - keep_sscs - - - - - - - - - - - -**What it does** - -This is for processing duplex sequencing data. It creates single-strand and duplex consensus reads from aligned read families. - ------ - -**Input** - -This expects the output format of the "Align families" tool. - ------ - -**Output** - -This will output final, duplex consensus reads in two FASTA files (first and second reads in the pairs). Optionally, you can save the single-strand reads too, in a separate FASTA file. - - - diff -r ba81823ad9e1 -r 197fa659cdfa make_families.xml --- a/make_families.xml Tue Dec 08 08:57:34 2015 -0500 +++ b/make_families.xml Mon Dec 21 14:33:58 2015 -0500 @@ -1,8 +1,8 @@ - + of duplex sequencing reads - duplex + duplex DUPLEX_DIR diff -r ba81823ad9e1 -r 197fa659cdfa tool_dependencies.xml --- a/tool_dependencies.xml Tue Dec 08 08:57:34 2015 -0500 +++ b/tool_dependencies.xml Mon Dec 21 14:33:58 2015 -0500 @@ -3,10 +3,10 @@ - + - https://github.com/makrutenko/duplex/archive/v0.2.tar.gz + https://github.com/makrutenko/dunovo/archive/v0.3.tar.gz make .