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planemo upload for repository https://github.com/morinlab/tools-morinlab/tree/master/tools/delly commit 4ef2d91b7c1686a2696b92fe538d4aec51d05e40-dirty
| author | morinlab |
|---|---|
| date | Tue, 11 Oct 2016 14:20:05 -0400 |
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<tool id="delly" name="DELLY" version="0.6.1"> <description>structural variant discovery by integrated paired-end and split-read analysis</description> <macros> <import>citations.xml</import> </macros> <requirements> <requirement type="package" version="1.55.0">boost</requirement> <requirement type="package" version="0.6.1">delly</requirement> <requirement type="set_environment">DELLY_DIR</requirement> <requirement type="set_environment">BOOST_ROOT_DIR"</requirement> <requirement type="set_environment">LD_LIBRARY_PATH"</requirement> <requirement type="set_environment">LIBRARY_PATH"</requirement> <requirement type="set_environment">CPLUS_INCLUDE_PATH"</requirement> <requirement type="set_environment">C_INCLUDE_PATH"</requirement> </requirements> <command detect_errors="aggressive"> <!-- BAM and BAI linking, (1) link BAM to new BAM file & (2) link BAM metadata to new BAI file --> #for $i, $s in enumerate( $repeatBam ) ln -s $s.sortedBam ./input$(i).bam; ln -s $s.sortedBam.metadata.bam_index ./input$(i).bam.bai; #end for <!-- Sets args to a list of types selected --> #if not isinstance( $variant_source_selector_param.value, list ): #set $args = [ $variant_source_selector_param.value ] #else: #set $args = $variant_source_selector_param.value #end if <!-- Run Delly Jobs for each type selected --> #for $option in $args \$DELLY_DIR/src/delly -t $option -o ./output.$(option).vcf -q $advancedsettings.mapQual -s $advancedsettings.madCutoff #if $option == "DEL": -m $advancedsettings.minFlank #end if -u $advancedsettings.genoQual #if $advancedsettings.vcfgeno -v $advancedsettings.vcfgeno #end if #if $reference_source.reference_source_selector_param == "cached": -g "${reference_source.reference.fields.path}" #else: -g $reference_source.reference #end if <!-- add each input bam to command --> #for $i, $s in enumerate( $repeatBam ): ./input$(i).bam #end for ; #end for <!-- Combine VCF Files and Sort Lexographically --> #set $option = $args[0] grep ^\# output.$(option).vcf > $outfile; grep ^\# -v output.$(option).vcf > variants.txt; <!-- If we called more than a single variant type, concatenate all the other types variant output --> #if isinstance( $variant_source_selector_param.value, list ): #for $option in $args[1:] grep ^\# -v output.$(option).vcf >> variants.txt; #end for #end if <!-- Sort all variant output, assuming that it will sort lexographically by chromosome, then position, ID --> <!-- In future, maybe develop a script to sort by bam header --> sort -k1,1d -k2,2n -k3,3d variants.txt > sortedVariants.txt; <!-- Filter Variants that have Passed Quality Checks --> #if $filterCalls awk '{if ($7 == "PASS") print $0;}' sortedVariants.txt >> $outfile; #else cat sortedVariants.txt >> $outfile; #end if </command> <inputs> <!-- REFERENCE OPTIONS --> <conditional name="reference_source"> <param type="select" name="reference_source_selector_param" label="Choose the source for the reference genome"> <option value="cached" selected="True">Use a built-in genome</option> <option value="history">Use a genome from the history</option> </param> <when value="cached"> <param type="select" name="reference" label="Genome"> <options from_data_table="all_fasta"/> </param> </when> <when value="history"> <param type="data" format="fasta" name="reference" label="Genome"/> </when> </conditional> <!-- VARIANT OPTIONS --> <param type="select" multiple="True" name="variant_source_selector_param" label="Select variants to identify in samples"> <option value="DEL" selected="true">Deletions</option> <option value="DUP">Duplications</option> <option value="INV">Inversions</option> <option value="TRA">Translocations</option> </param> <!-- <param name="interval_file" type="data" format="txt" optional="true" label="Interval file" help="Created by make parallel, only use when parallelism is turned on, note interchromosomal and intrachromosomal events have different interval files"/> --> <repeat name="repeatBam" title="Bam Alignment" min="1" default="1" > <param format="bam" name="sortedBam" type="data" label="File" /> </repeat> <!-- <param name="excludeFile" type="data" format="bed" optional="true" label="Chromosomes to Exclude"/> --> <param name="filterCalls" type="boolean" value="false" label="Filter Poor Variant Calls"/> <section name="advancedsettings" title="Advanced Settings" expanded="false"> <!-- Paired End Options --> <param name="mapQual" type="integer" value="0" min="0" max="255" label="PE - Minimum Mapping Quality" /> <param name="madCutoff" type="integer" value="9" min="0" max="255" label="PE - Insert Size Cutoff" /> <!-- SR Options --> <param name="minFlank" type="integer" value="13" label="SR - Minimum Flanking Sequence" /> <!-- Genotyping Options --> <param format="vcf" name="vcfgeno" type="data" optional="true" label="GT - Input VCF" /> <param name="genoQual" type="integer" value="20" min="0" max="255" label="GT - Minimum Mapping Quality" /> </section> </inputs> <outputs> <data format="vcf" name="outfile" /> </outputs> <citations> <expand macro="morinlab_citation"/> <expand macro="galaxy_citation"/> <expand macro="delly_citation"/> </citations> </tool>
