diff rmarkdown_deseq2_count_matrix.xml @ 0:867a77a7a380 draft

planemo upload
author mingchen0919
date Fri, 29 Dec 2017 23:37:30 -0500
parents
children 4a7131658ca6
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/rmarkdown_deseq2_count_matrix.xml	Fri Dec 29 23:37:30 2017 -0500
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+<tool id="rmarkdown_deseq2_count_matrix" name="DESeq2 (count matrix)" version="1.0.0">
+  <requirements>
+    <requirement type="package" version="1.15.0.6-0">pandoc</requirement>
+    <requirement type="package" version="1.20.0">r-getopt</requirement>
+    <requirement type="package" version="1.3">r-rmarkdown</requirement>
+    <requirement type="package" version="0.3.5">r-htmltools</requirement>
+    <requirement type="package" version="0.5.0">r-dplyr</requirement>
+    <requirement type="package" version="1.14.1">bioconductor-deseq2</requirement>
+    <requirement type="package" version="0.2">r-dt</requirement>
+  </requirements>
+  <description>deseq2 analysis given a count matrix input</description>
+  <stdio>
+    <regex source="stderr" match="XXX" level="warning" description="Check the warnings_and_errors.txt file for more details."/>
+  </stdio>
+  <command><![CDATA[                    
+Rscript '${__tool_directory__}/rmarkdown_deseq2_count_matrx_render.R'
+                        -e $echo
+                        -c $count_matrix
+                        -r $report
+                        -d $report.files_path
+                        -s $sink_message
+                        -t '${__tool_directory__}/rmarkdown_deseq2_count_matrx.Rmd'
+                    ]]></command>
+  <inputs>
+    <param type="boolean" name="echo" label="Display analysis code in report?" optional="False" checked="False" truevalue="TRUE" falsevalue="FALSE"/>
+    <param type="data" name="count_matrix" label="count matrix" optional="False" format="txt,csv" multiple="False"/>
+  </inputs>
+  <outputs>
+    <data name="report" format="html" label="DESeq2 report" hidden="false"/>
+    <data name="differential_genes" format="csv" label="differential genes" from_work_dir="differential_genes.csv" hidden="false"/>
+    <data name="sink_message" format="txt" label="Warnings and Errors" from_work_dir="warnings_and_errors.txt" hidden="false"/>
+  </outputs>
+  <help><![CDATA[See http://bioconductor.org/packages/devel/bioc/vignettes/DESeq2/inst/doc/DESeq2.html]]></help>
+  <citations>
+    <citation type="bibtex"><![CDATA[            @article{love2014moderated,
+            title={Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2},
+            author={Love, Michael I and Huber, Wolfgang and Anders, Simon},
+            journal={Genome biology},
+            volume={15},
+            number={12},
+            pages={550},
+            year={2014},
+            publisher={BioMed Central}
+            }]]></citation>
+    <citation type="bibtex"><![CDATA[            @article{allaire2016rmarkdown,
+            title={rmarkdown: Dynamic Documents for R, 2016},
+            author={Allaire, J and Cheng, Joe and Xie, Yihui and McPherson, Jonathan and Chang, Winston and Allen, Jeff
+            and Wickham, Hadley and Atkins, Aron and Hyndman, Rob},
+            journal={R package version 0.9},
+            volume={6},
+            year={2016}
+            }]]></citation>
+  </citations>
+</tool>