diff DESeq_results_render.R @ 0:6b12f3dc358a draft

planemo upload

--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/DESeq_results_render.R	Tue Feb 27 13:04:12 2018 -0500
@@ -0,0 +1,108 @@
+library(getopt)
+library(rmarkdown)
+library(htmltools)
+library(dplyr)
+library(DESeq2)
+library(pheatmap)
+library(genefilter)
+library(DT)
+library(stringi)
+library(RColorBrewer)
+library(ggplot2)
+
+##============ Sink warnings and errors to a file ==============
+## use the sink() function to wrap all code within it.
+##==============================================================
+zz = file('warnings_and_errors.txt')
+sink(zz)
+sink(zz, type = 'message')
+  ##---------below is the code for rendering .Rmd templates-----
+  
+  ##=============STEP 1: handle command line arguments==========
+  ##
+  ##============================================================
+  # column 1: the long flag name
+  # column 2: the short flag alias. A SINGLE character string
+  # column 3: argument mask
+  #           0: no argument
+  #           1: argument required
+  #           2: argument is optional
+  # column 4: date type to which the flag's argument shall be cast.
+  #           possible values: logical, integer, double, complex, character.
+  #-------------------------------------------------------------
+  #++++++++++++++++++++ Best practice ++++++++++++++++++++++++++
+  # 1. short flag alias should match the flag in the command section in the XML file.
+  # 2. long flag name can be any legal R variable names
+  # 3. two names in args_list can have common string but one name should not be a part of another name.
+  #    for example, one name is "ECHO", if another name is "ECHO_XXX", it will cause problems.
+  #+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
+  args_list=list()
+  ##------- 1. input data ---------------------
+  args_list$ECHO = c('echo', 'e', '1', 'character')
+  args_list$DESEQ_WORKSPACE = c('deseq_workspace', 'W', '1', 'character')
+  args_list$CONTRAST_FACTOR = c('contrast_factor', 'C', '1', 'character')
+  args_list$TREATMENT_LEVEL = c('treatment_level', 'T', '1', 'character')
+  args_list$CONDITION_LEVEL = c('condition_level', 'K', '1', 'character')
+  args_list$CLUSTERING_FACTORS = c('clustering_factors', 'M', '1', 'character')
+  ##--------2. output report and outputs --------------
+  args_list$REPORT_HTML = c('report_html', 'r', '1', 'character')
+  args_list$REPORT_DIR = c('report_dir', 'd', '1', 'character')
+  args_list$SINK_MESSAGE = c('sink_message', 's', '1', 'character')
+  args_list$DESEQ_RESULTS = c('deseq_results', 'R', '1', 'character')
+  ##--------3. .Rmd templates in the tool directory ----------
+  args_list$deseq_results_RMD = c('deseq_results_rmd', 't', '1', 'character')
+  ##-----------------------------------------------------------
+  opt = getopt(t(as.data.frame(args_list)))
+
+
+  
+  ##=======STEP 2: create report directory (optional)==========
+  ##
+  ##===========================================================
+  dir.create(opt$report_dir)
+  
+  ##=STEP 3: replace placeholders in .Rmd with argument values=
+  ##
+  ##===========================================================
+  #++ need to replace placeholders with args values one by one+
+  readLines(opt$deseq_results_rmd) %>%
+    (function(x) {
+      gsub('ECHO', opt$echo, x)
+    }) %>%
+    (function(x) {
+      gsub('DESEQ_WORKSPACE', opt$deseq_workspace, x)
+    }) %>%
+    (function(x) {
+      gsub('CONTRAST_FACTOR', opt$contrast_factor, x)
+    }) %>%
+    (function(x) {
+      gsub('TREATMENT_LEVEL', opt$treatment_level, x)
+    }) %>%
+    (function(x) {
+      gsub('CONDITION_LEVEL', opt$condition_level, x)
+    }) %>%
+    (function(x) {
+      gsub('CLUSTERING_FACTORS', opt$clustering_factors, x)
+    }) %>%
+    (function(x) {
+      gsub('REPORT_DIR', opt$report_dir, x)
+    }) %>%
+    (function(x) {
+      gsub('DESEQ_RESULTS', opt$deseq_results, x)
+    }) %>%
+    (function(x) {
+      fileConn = file('deseq_results.Rmd')
+      writeLines(x, con=fileConn)
+      close(fileConn)
+    })
+  
+
+  ##=============STEP 4: render .Rmd templates=================
+  ##
+  ##===========================================================
+  render('deseq_results.Rmd', output_file = opt$report_html)
+
+
+  ##--------end of code rendering .Rmd templates----------------
+sink()
+##=========== End of sinking output=============================
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