# HG changeset patch # User mingchen0919 # Date 1508043010 14400 # Node ID fd15cf620d5dec33359c9dac8305a24ddff7b701 # Parent 89cc5b0264941082cb83670231e744a680cf5cb5 planemo upload for repository https://github.com/statonlab/docker-GRReport/tree/master/my_tools/rmarkdown_bdss_client commit 3d2a2c46f10b7f00a36771d2a0fc334d2c177730-dirty diff -r 89cc5b026494 -r fd15cf620d5d bdss_client.Rmd --- a/bdss_client.Rmd Sat Oct 14 22:59:06 2017 -0400 +++ b/bdss_client.Rmd Sun Oct 15 00:50:10 2017 -0400 @@ -41,7 +41,7 @@ # loop through SRA accessions to download and extract reads. for(url in urls) { print(url) - bdss_command = paste0('/tool_deps/_conda/bin/bdss transfer --destination read_files_directory -u ', url) + bdss_command = paste0('/main/sites/galaxy/galaxy/tools/_conda/bin/bdss transfer --destination read_files_directory -u ', url) print(bdss_command) print(system(bdss_command, intern = TRUE)) } diff -r 89cc5b026494 -r fd15cf620d5d bdss_client_sra.Rmd --- a/bdss_client_sra.Rmd Sat Oct 14 22:59:06 2017 -0400 +++ b/bdss_client_sra.Rmd Sun Oct 15 00:50:10 2017 -0400 @@ -46,7 +46,7 @@ substr(id, 1, 3), '/', substr(id, 1, 6), '/', id, '/', id, '.sra') # download sra file with bdss - bdss_command = paste0('/tool_deps/_conda/bin/bdss transfer -u ', url) + bdss_command = paste0('/main/sites/galaxy/galaxy/tools/_conda/bin/bdss transfer -u ', url) system(bdss_command, intern = TRUE) # convert .sra to .fastq/.fasta if('FORMAT' == 'fasta') { @@ -68,7 +68,7 @@ substr(id, 1, 3), '/', substr(id, 1, 6), '/', id, '/', id, '.sra') # download sra file with bdss - bdss_command = paste0('/tool_deps/_conda/bin/bdss transfer -u ', url) + bdss_command = paste0('/main/sites/galaxy/galaxy/tools/_conda/bin/bdss transfer -u ', url) system(bdss_command, intern = TRUE) # convert .sra to .fastq/.fasta if('FORMAT' == 'fasta') { @@ -79,9 +79,9 @@ cat('----convert SRA to fastq/fasta------\n') command_stdout = system(command, intern = TRUE) print(command_stdout) - if(length(command_stdout) < 3) { + if(!(paste0(id, '_2.FORMAT') %in% list.files('pe_read_files_directory'))) { # this is not a paired end SRA file. The corresponding file will be deleted. - cat(paste0(id, 'is not paired end SRA, the corresponding fastq/fasta file will deleted.')) + cat(paste0(id, ' is not paired end SRA, the corresponding fastq/fasta file will deleted.')) system(paste0('rm pe_read_files_directory/', id, '_1.*'), intern = TRUE) } @@ -95,8 +95,10 @@ cat('-----Renaming files------\n') # rename files for paired end reads old_files = paste0('./pe_read_files_directory/', list.files('./pe_read_files_directory')) +print(old_files) new_files = gsub('_1', '_forward', old_files) new_files = gsub('_2', '_reverse', new_files) +print(new_files) file.rename(old_files, new_files) ``` diff -r 89cc5b026494 -r fd15cf620d5d bdss_client_sra.xml --- a/bdss_client_sra.xml Sat Oct 14 22:59:06 2017 -0400 +++ b/bdss_client_sra.xml Sun Oct 15 00:50:10 2017 -0400 @@ -10,7 +10,8 @@ r-rcurl - Download data with BDSS client and generate list (single end SRA data) and list:paired dataset collection (paired end SRA data). + Download data with BDSS client and generate list (single end SRA data) and list:paired dataset collection + (paired end SRA data). @@ -35,10 +36,10 @@ ]]> - - + + - + - +