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author matthias
date Mon, 27 May 2019 05:35:49 -0400
parents e76f2213f0cc
children fabe0d136752
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e76f2213f0cc planemo upload for repository https://github.com/bernt-matthias/mb-galaxy-tools/tree/topic/dada2/tools/dada2 commit 990192685955e9cda0282e348c28ef6462d88a38
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1 Wrappers for the core functionality of the dada2 package https://benjjneb.github.io/dada2/index.html.
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3 - filterAndTrim
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4 - derep
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5 - learnErrors
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6 - dada
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7 - mergePairs
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8 - makeSequenceTable
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9 - removeBimeraDenovo
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11 Datatypes
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12 =========
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e76f2213f0cc planemo upload for repository https://github.com/bernt-matthias/mb-galaxy-tools/tree/topic/dada2/tools/dada2 commit 990192685955e9cda0282e348c28ef6462d88a38
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14 The dada2 Galaxy wrappers use a few extra data types to ensure that only inputs of the correct type can be used.
e76f2213f0cc planemo upload for repository https://github.com/bernt-matthias/mb-galaxy-tools/tree/topic/dada2/tools/dada2 commit 990192685955e9cda0282e348c28ef6462d88a38
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e76f2213f0cc planemo upload for repository https://github.com/bernt-matthias/mb-galaxy-tools/tree/topic/dada2/tools/dada2 commit 990192685955e9cda0282e348c28ef6462d88a38
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16 For the outputs of derep, dada, learnErrors, and mergePairs the following datatypes are used that derive from Rdata (which contains the named list that is returned from the corresponding dada function):
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18 - dada2_derep (Rdata: named list see docs for derep-class)
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19 - dada2_dada (Rdata: named list, see docs for dada-class)
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20 - dada2_errorrates (Rdata: named list, see docs for learnErrors)
e76f2213f0cc planemo upload for repository https://github.com/bernt-matthias/mb-galaxy-tools/tree/topic/dada2/tools/dada2 commit 990192685955e9cda0282e348c28ef6462d88a38
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21 - dada2_mergepairs (Rdata: named list, see docs for mergePairs)
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23 For the outputs of makeSequenceTable and removeBimeraDenovo the following data types are used which derive from tabular:
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25 - dada2_uniques
e76f2213f0cc planemo upload for repository https://github.com/bernt-matthias/mb-galaxy-tools/tree/topic/dada2/tools/dada2 commit 990192685955e9cda0282e348c28ef6462d88a38
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26 -- in R a named integer vector (names are the unique sequences)
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27 -- in Galaxy written as a table (each row corresponding to a unique sequence, column 1: the sequence, column 2: the count)
e76f2213f0cc planemo upload for repository https://github.com/bernt-matthias/mb-galaxy-tools/tree/topic/dada2/tools/dada2 commit 990192685955e9cda0282e348c28ef6462d88a38
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28 - dada2_sequencetable
e76f2213f0cc planemo upload for repository https://github.com/bernt-matthias/mb-galaxy-tools/tree/topic/dada2/tools/dada2 commit 990192685955e9cda0282e348c28ef6462d88a38
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29 -- in R a named integer matrix (rows = samples, columns = unique sequences)
e76f2213f0cc planemo upload for repository https://github.com/bernt-matthias/mb-galaxy-tools/tree/topic/dada2/tools/dada2 commit 990192685955e9cda0282e348c28ef6462d88a38
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30 -- in Galaxy written as a table (rows = unique sequences, columns = samples)
e76f2213f0cc planemo upload for repository https://github.com/bernt-matthias/mb-galaxy-tools/tree/topic/dada2/tools/dada2 commit 990192685955e9cda0282e348c28ef6462d88a38
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32 Note the difference between the R and Galaxy representations! The main motivation is that the dada2_sequencetable is analogous to OTU tables as produced for instance by qiime (and it seemed natural to extend this to the uniques which are essentially a sequencetables of single samples).
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35 TODOs
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36 =====
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38 - implememt getUniques tool to view intermediate results?
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39 - implement tests for cached reference data