Mercurial > repos > mahtabm > ensembl
diff variant_effect_predictor/Bio/SeqI.pm @ 0:1f6dce3d34e0
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author | mahtabm |
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date | Thu, 11 Apr 2013 02:01:53 -0400 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/variant_effect_predictor/Bio/SeqI.pm Thu Apr 11 02:01:53 2013 -0400 @@ -0,0 +1,243 @@ +# $Id: SeqI.pm,v 1.25 2002/12/05 13:46:30 heikki Exp $ +# +# BioPerl module for Bio::SeqI +# +# Cared for by Ewan Birney <birney@ebi.ac.uk> +# +# Copyright Ewan Birney +# +# You may distribute this module under the same terms as perl itself + +# POD documentation - main docs before the code + +=head1 NAME + +Bio::SeqI [Developers] - Abstract Interface of Sequence (with features) + +=head1 SYNOPSIS + + # Bio::SeqI is the interface class for sequences. + + # If you are a newcomer to bioperl, you should + # start with Bio::Seq documentation. This + # documentation is mainly for developers using + # Bioperl. + + # Bio::SeqI implements Bio::PrimarySeqI + $seq = $seqobj->seq(); # actual sequence as a string + $seqstr = $seqobj->subseq(10,50); + + # Bio::SeqI has annotationcollections + + $ann = $seqobj->annotation(); # annotation object + + # Bio::SeqI has sequence features + # features must implement Bio::SeqFeatureI + + @features = $seqobj->get_SeqFeatures(); # just top level + @features = $seqobj->get_all_SeqFeatures(); # descend into sub features + + + +=head1 DESCRIPTION + +Bio::SeqI is the abstract interface of annotated Sequences. These +methods are those which you can be guarenteed to get for any Bio::SeqI +- for most users of the package the documentation (and methods) in +this class are not at useful - this is a developers only class which +defines what methods have to be implmented by other Perl objects to +comply to the Bio::SeqI interface. Go "perldoc Bio::Seq" or "man +Bio::Seq" for more information. + + +There aren't many here, because too many complicated functions here +prevent implementations which are just wrappers around a database or +similar delayed mechanisms. + +Most of the clever stuff happens inside the SeqFeatureI system. + +A good reference implementation is Bio::Seq which is a pure perl +implementation of this class with alot of extra pieces for extra +manipulation. However, if you want to be able to use any sequence +object in your analysis, if you can do it just using these methods, +then you know you will be future proof and compatible with other +implementations of Seq. + +=head1 FEEDBACK + +=head2 Mailing Lists + +User feedback is an integral part of the evolution of this and other +Bioperl modules. Send your comments and suggestions preferably to one +of the Bioperl mailing lists. Your participation is much appreciated. + + bioperl-l@bioperl.org - General discussion + http://bio.perl.org/MailList.html - About the mailing lists + +=head2 Reporting Bugs + +Report bugs to the Bioperl bug tracking system to help us keep track +the bugs and their resolution. Bug reports can be submitted via email +or the web: + + bioperl-bugs@bio.perl.org + http://bugzilla.bioperl.org/ + +=head1 AUTHOR - Ewan Birney + +Email birney@sanger.ac.uk + + +=head1 APPENDIX + +The rest of the documentation details each of the object +methods. Internal methods are usually preceded with a _ + +=cut + +#' +# Let the code begin... + + +package Bio::SeqI; +use strict; + +use vars qw(@ISA); +use Bio::PrimarySeqI; +use Bio::AnnotatableI; +use Bio::FeatureHolderI; + +# Object preamble - inheriets from Bio::PrimarySeqI + +@ISA = qw(Bio::PrimarySeqI Bio::AnnotatableI Bio::FeatureHolderI); + +=head2 get_SeqFeatures + + Title : get_SeqFeatures + Usage : my @feats = $seq->get_SeqFeatures(); + Function: retrieve just the toplevel sequence features attached to this seq + Returns : array of Bio::SeqFeatureI objects + Args : none + +This method comes through extension of Bio::FeatureHolderI. See +L<Bio::FeatureHolderI> and L<Bio::SeqFeatureI> for more information. + +=cut + +=head2 get_all_SeqFeatures + + Title : get_all_SeqFeatures + Usage : @features = $annseq->get_all_SeqFeatures() + Function: returns all SeqFeatures, included sub SeqFeatures + Returns : an array of Bio::SeqFeatureI objects + Args : none + +This method comes through extension of Bio::FeatureHolderI. See +L<Bio::FeatureHolderI> and L<Bio::SeqFeatureI> for more information. + +=cut + +=head2 feature_count + + Title : feature_count + Usage : $seq->feature_count() + Function: Return the number of SeqFeatures attached to a sequence + Returns : integer representing the number of SeqFeatures + Args : none + +This method comes through extension of Bio::FeatureHolderI. See +L<Bio::FeatureHolderI> for more information. + +=cut + +=head2 seq + + Title : seq + Usage : my $string = $seq->seq(); + Function: Retrieves the sequence string for the sequence object + Returns : string + Args : none + + +=cut + +sub seq{ + my ($self) = @_; + $self->throw_not_implemented(); +} + +=head2 write_GFF + + Title : write_GFF + Usage : $seq->write_GFF(\*FILEHANDLE); + Function: Convience method to write out all the sequence features + in GFF format to the provided filehandle (STDOUT by default) + Returns : none + Args : [optional] filehandle to write to (default is STDOUT) + + +=cut + +sub write_GFF{ + my ($self,$fh) = @_; + + $fh || do { $fh = \*STDOUT; }; + + foreach my $sf ( $self->get_all_SeqFeatures() ) { + print $fh $sf->gff_string, "\n"; + } + +} + +=head2 annotation + + Title : annotation + Usage : $obj->annotation($seq_obj) + Function: retrieve the attached annotation object + Returns : Bio::AnnotationCollectionI or none; + +See L<Bio::AnnotationCollectionI> and L<Bio::Annotation::Collection> +for more information. This method comes through extension from +L<Bio::AnnotatableI>. + +=cut + +=head2 species + + Title : species + Usage : + Function: Gets or sets the species + Example : $species = $self->species(); + Returns : Bio::Species object + Args : Bio::Species object or none; + +See L<Bio::Species> for more information + +=cut + +sub species { + my ($self) = @_; + $self->throw_not_implemented(); +} + +=head2 primary_seq + + Title : primary_seq + Usage : $obj->primary_seq($newval) + Function: Retrieve the underlying Bio::PrimarySeqI object if available. + This is in the event one has a sequence with lots of features + but want to be able to narrow the object to just one with + the basics of a sequence (no features or annotations). + Returns : Bio::PrimarySeqI + Args : Bio::PrimarySeqI or none; + +See L<Bio::PrimarySeqI> for more information + +=cut + +sub primary_seq { + my ($self) = @_; + $self->throw_not_implemented; +} + +1;