comparison variant_effect_predictor/Bio/SeqIO/raw.pm @ 0:1f6dce3d34e0

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date Thu, 11 Apr 2013 02:01:53 -0400
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1 #-----------------------------------------------------------------------------
2 # PACKAGE : Bio::SeqIO::raw
3 # AUTHOR : Ewan Birney <birney@ebi.ac.uk>
4 # CREATED : Feb 16 1999
5 # REVISION: $Id: raw.pm,v 1.15.2.1 2003/02/05 21:55:21 jason Exp $
6 #
7 # Copyright (c) 1997-9 bioperl, Ewan Birney. All Rights Reserved.
8 # This module is free software; you can redistribute it and/or
9 # modify it under the same terms as Perl itself.
10 #
11 # _History_
12 #
13 # Ewan Birney <birney@ebi.ac.uk> developed the SeqIO
14 # schema and the first prototype modules.
15 #
16 # This code is based on his Bio::SeqIO::Fasta module with
17 # the necessary minor tweaks necessary to get it to read
18 # and write raw formatted sequences made by
19 # chris dagdigian <dag@sonsorol.org>
20 #
21 # October 18, 1999 Largely rewritten by Lincoln Stein
22 #
23 # Copyright Ewan Birney
24 #
25 # You may distribute this module under the same terms as perl itself
26
27 # POD documentation - main docs before the code
28
29 =head1 NAME
30
31 Bio::SeqIO::raw - raw sequence file input/output stream
32
33 =head1 SYNOPSIS
34
35 Do not use this module directly. Use it via the L<Bio::SeqIO> class.
36
37 =head1 DESCRIPTION
38
39 This object can transform Bio::Seq objects to and from raw flat
40 file databases.
41
42
43 =head1 FEEDBACK
44
45 =head2 Mailing Lists
46
47 User feedback is an integral part of the evolution of this
48 and other Bioperl modules. Send your comments and suggestions preferably
49 to one of the Bioperl mailing lists.
50 Your participation is much appreciated.
51
52 bioperl-l@bioperl.org - General discussion
53 http://www.bioperl.org/MailList.shtml - About the mailing lists
54
55 =head2 Reporting Bugs
56
57 Report bugs to the Bioperl bug tracking system to help us keep track
58 the bugs and their resolution.
59 Bug reports can be submitted via email or the web:
60
61 bioperl-bugs@bio.perl.org
62 http://bugzilla.bioperl.org/
63
64 =head1 AUTHORS
65
66 Ewan Birney E<lt>birney@ebi.ac.ukE<gt>
67 Lincoln Stein E<lt>lstein@cshl.orgE<gt>
68
69 =head1 CONTRIBUTORS
70
71 Jason Stajich E<lt>jason@bioperl.org<gt>
72
73 =head1 APPENDIX
74
75 The rest of the documentation details each of the object methods.
76 Internal methods are usually preceded with a _
77
78 =cut
79
80
81 # Let the code begin...
82
83 package Bio::SeqIO::raw;
84 use strict;
85 use vars qw(@ISA);
86
87 use Bio::SeqIO;
88 use Bio::Seq::SeqFactory;
89
90 @ISA = qw(Bio::SeqIO);
91
92 sub _initialize {
93 my($self,@args) = @_;
94 $self->SUPER::_initialize(@args);
95 if( ! defined $self->sequence_factory ) {
96 $self->sequence_factory(new Bio::Seq::SeqFactory
97 (-verbose => $self->verbose(),
98 -type => 'Bio::Seq'));
99 }
100 }
101
102 =head2 next_seq
103
104 Title : next_seq
105 Usage : $seq = $stream->next_seq()
106 Function: returns the next sequence in the stream
107 Returns : Bio::Seq object
108 Args :
109
110
111 =cut
112
113 sub next_seq{
114 my ($self,@args) = @_;
115 ## When its 1 sequence per line with no formatting at all,
116 ## grabbing it should be easy :)
117
118 my $nextline = $self->_readline();
119 if( !defined $nextline ){ return undef; }
120
121 my $sequence = uc($nextline);
122 $sequence =~ s/\W//g;
123
124 return $self->sequence_factory->create(-seq => $sequence);
125 }
126
127 =head2 write_seq
128
129 Title : write_seq
130 Usage : $stream->write_seq($seq)
131 Function: writes the $seq object into the stream
132 Returns : 1 for success and 0 for error
133 Args : Array of Bio::PrimarySeqI objects
134
135
136 =cut
137
138 sub write_seq {
139 my ($self,@seq) = @_;
140 foreach my $seq (@seq) {
141 $self->throw("Must provide a valid Bio::PrimarySeqI object")
142 unless defined $seq && ref($seq) && $seq->isa('Bio::PrimarySeqI');
143 $self->_print($seq->seq, "\n") or return;
144 }
145 $self->flush if $self->_flush_on_write && defined $self->_fh;
146 return 1;
147 }
148
149 =head2 write_qual
150
151 Title : write_qual
152 Usage : $stream->write_qual($seq)
153 Function: writes the $seq object into the stream
154 Returns : 1 for success and 0 for error
155 Args : Bio::Seq object
156
157
158 =cut
159
160 sub write_qual {
161 my ($self,@seq) = @_;
162 my @qual = ();
163 foreach (@seq) {
164 unless ($_->isa("Bio::Seq::SeqWithQuality")){
165 warn("You cannot write raw qualities without supplying a Bio::Seq::SeqWithQuality object! You passed a ", ref($_), "\n");
166 next;
167 }
168 @qual = @{$_->qual};
169 if(scalar(@qual) == 0) {
170 $qual[0] = "\n";
171 }
172
173 $self->_print (join " ", @qual,"\n") or return;
174
175 }
176 return 1;
177 }
178 1;