Mercurial > repos > mahtabm > ensembl
comparison variant_effect_predictor/Bio/SeqIO/ctf.pm @ 0:1f6dce3d34e0
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| author | mahtabm |
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| date | Thu, 11 Apr 2013 02:01:53 -0400 |
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| -1:000000000000 | 0:1f6dce3d34e0 |
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| 1 # $Id: ctf.pm,v 1.8 2002/10/22 07:38:42 lapp Exp $ | |
| 2 # BioPerl module for Bio::SeqIO::ctf | |
| 3 # | |
| 4 # Cared for by Aaron Mackey <amackey@virginia.edu> | |
| 5 # | |
| 6 # Copyright Aaron Mackey | |
| 7 # | |
| 8 # You may distribute this module under the same terms as perl itself | |
| 9 | |
| 10 # POD documentation - main docs before the code | |
| 11 | |
| 12 =head1 NAME | |
| 13 | |
| 14 Bio::SeqIO::ctf - ctf trace sequence input/output stream | |
| 15 | |
| 16 =head1 SYNOPSIS | |
| 17 | |
| 18 Do not use this module directly. Use it via the Bio::SeqIO class. | |
| 19 | |
| 20 =head1 DESCRIPTION | |
| 21 | |
| 22 This object can transform Bio::Seq objects to and from ctf trace | |
| 23 files. | |
| 24 | |
| 25 =head1 FEEDBACK | |
| 26 | |
| 27 =head2 Mailing Lists | |
| 28 | |
| 29 User feedback is an integral part of the evolution of this and other | |
| 30 Bioperl modules. Send your comments and suggestions preferably to one | |
| 31 of the Bioperl mailing lists. Your participation is much appreciated. | |
| 32 | |
| 33 bioperl-l@bioperl.org - General discussion | |
| 34 http://bioperl.org/MailList.shtml - About the mailing lists | |
| 35 | |
| 36 =head2 Reporting Bugs | |
| 37 | |
| 38 Report bugs to the Bioperl bug tracking system to help us keep track | |
| 39 the bugs and their resolution. | |
| 40 Bug reports can be submitted via email or the web: | |
| 41 | |
| 42 bioperl-bugs@bio.perl.org | |
| 43 http://bugzilla.bioperl.org/ | |
| 44 | |
| 45 =head1 AUTHORS - Aaron Mackey | |
| 46 | |
| 47 Email: amackey@virginia.edu | |
| 48 | |
| 49 =head1 APPENDIX | |
| 50 | |
| 51 The rest of the documentation details each of the object | |
| 52 methods. Internal methods are usually preceded with a _ | |
| 53 | |
| 54 =cut | |
| 55 | |
| 56 # Let the code begin... | |
| 57 | |
| 58 package Bio::SeqIO::ctf; | |
| 59 use vars qw(@ISA $READ_AVAIL); | |
| 60 use strict; | |
| 61 # Object preamble - inherits from Bio::Root::Object | |
| 62 | |
| 63 use Bio::SeqIO; | |
| 64 use Bio::Seq::SeqFactory; | |
| 65 | |
| 66 push @ISA, qw( Bio::SeqIO ); | |
| 67 | |
| 68 sub BEGIN { | |
| 69 eval { require Bio::SeqIO::staden::read; }; | |
| 70 if ($@) { | |
| 71 $READ_AVAIL = 0; | |
| 72 } else { | |
| 73 push @ISA, "Bio::SeqIO::staden::read"; | |
| 74 $READ_AVAIL = 1; | |
| 75 } | |
| 76 } | |
| 77 | |
| 78 sub _initialize { | |
| 79 my($self,@args) = @_; | |
| 80 $self->SUPER::_initialize(@args); | |
| 81 if( ! defined $self->sequence_factory ) { | |
| 82 $self->sequence_factory(new Bio::Seq::SeqFactory(-verbose => $self->verbose(), -type => 'Bio::Seq::SeqWithQuality')); | |
| 83 } | |
| 84 unless ($READ_AVAIL) { | |
| 85 Bio::Root::Root->throw( -class => 'Bio::Root::SystemException', | |
| 86 -text => "Bio::SeqIO::staden::read is not available; make sure the bioperl-ext package has been installed successfully!" | |
| 87 ); | |
| 88 } | |
| 89 } | |
| 90 | |
| 91 =head2 next_seq | |
| 92 | |
| 93 Title : next_seq | |
| 94 Usage : $seq = $stream->next_seq() | |
| 95 Function: returns the next sequence in the stream | |
| 96 Returns : Bio::SeqWithQuality object | |
| 97 Args : NONE | |
| 98 | |
| 99 =cut | |
| 100 | |
| 101 sub next_seq { | |
| 102 | |
| 103 my ($self) = @_; | |
| 104 | |
| 105 my ($seq, $id, $desc, $qual) = $self->read_trace($self->_fh, 'ctf'); | |
| 106 | |
| 107 # create the seq object | |
| 108 $seq = $self->sequence_factory->create(-seq => $seq, | |
| 109 -id => $id, | |
| 110 -primary_id => $id, | |
| 111 -desc => $desc, | |
| 112 -alphabet => 'DNA', | |
| 113 -qual => $qual | |
| 114 ); | |
| 115 return $seq; | |
| 116 } | |
| 117 | |
| 118 =head2 write_seq | |
| 119 | |
| 120 Title : write_seq | |
| 121 Usage : $stream->write_seq(@seq) | |
| 122 Function: writes the $seq object into the stream | |
| 123 Returns : 1 for success and 0 for error | |
| 124 Args : Bio::Seq object | |
| 125 | |
| 126 | |
| 127 =cut | |
| 128 | |
| 129 sub write_seq { | |
| 130 my ($self,@seq) = @_; | |
| 131 | |
| 132 my $fh = $self->_fh; | |
| 133 foreach my $seq (@seq) { | |
| 134 $self->write_trace($fh, $seq, 'ctf'); | |
| 135 } | |
| 136 | |
| 137 $self->flush if $self->_flush_on_write && defined $self->_fh; | |
| 138 return 1; | |
| 139 } | |
| 140 | |
| 141 1; |
