Mercurial > repos > mahtabm > ensembl
comparison variant_effect_predictor/Bio/SeqI.pm @ 0:1f6dce3d34e0
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| author | mahtabm |
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| date | Thu, 11 Apr 2013 02:01:53 -0400 |
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| -1:000000000000 | 0:1f6dce3d34e0 |
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| 1 # $Id: SeqI.pm,v 1.25 2002/12/05 13:46:30 heikki Exp $ | |
| 2 # | |
| 3 # BioPerl module for Bio::SeqI | |
| 4 # | |
| 5 # Cared for by Ewan Birney <birney@ebi.ac.uk> | |
| 6 # | |
| 7 # Copyright Ewan Birney | |
| 8 # | |
| 9 # You may distribute this module under the same terms as perl itself | |
| 10 | |
| 11 # POD documentation - main docs before the code | |
| 12 | |
| 13 =head1 NAME | |
| 14 | |
| 15 Bio::SeqI [Developers] - Abstract Interface of Sequence (with features) | |
| 16 | |
| 17 =head1 SYNOPSIS | |
| 18 | |
| 19 # Bio::SeqI is the interface class for sequences. | |
| 20 | |
| 21 # If you are a newcomer to bioperl, you should | |
| 22 # start with Bio::Seq documentation. This | |
| 23 # documentation is mainly for developers using | |
| 24 # Bioperl. | |
| 25 | |
| 26 # Bio::SeqI implements Bio::PrimarySeqI | |
| 27 $seq = $seqobj->seq(); # actual sequence as a string | |
| 28 $seqstr = $seqobj->subseq(10,50); | |
| 29 | |
| 30 # Bio::SeqI has annotationcollections | |
| 31 | |
| 32 $ann = $seqobj->annotation(); # annotation object | |
| 33 | |
| 34 # Bio::SeqI has sequence features | |
| 35 # features must implement Bio::SeqFeatureI | |
| 36 | |
| 37 @features = $seqobj->get_SeqFeatures(); # just top level | |
| 38 @features = $seqobj->get_all_SeqFeatures(); # descend into sub features | |
| 39 | |
| 40 | |
| 41 | |
| 42 =head1 DESCRIPTION | |
| 43 | |
| 44 Bio::SeqI is the abstract interface of annotated Sequences. These | |
| 45 methods are those which you can be guarenteed to get for any Bio::SeqI | |
| 46 - for most users of the package the documentation (and methods) in | |
| 47 this class are not at useful - this is a developers only class which | |
| 48 defines what methods have to be implmented by other Perl objects to | |
| 49 comply to the Bio::SeqI interface. Go "perldoc Bio::Seq" or "man | |
| 50 Bio::Seq" for more information. | |
| 51 | |
| 52 | |
| 53 There aren't many here, because too many complicated functions here | |
| 54 prevent implementations which are just wrappers around a database or | |
| 55 similar delayed mechanisms. | |
| 56 | |
| 57 Most of the clever stuff happens inside the SeqFeatureI system. | |
| 58 | |
| 59 A good reference implementation is Bio::Seq which is a pure perl | |
| 60 implementation of this class with alot of extra pieces for extra | |
| 61 manipulation. However, if you want to be able to use any sequence | |
| 62 object in your analysis, if you can do it just using these methods, | |
| 63 then you know you will be future proof and compatible with other | |
| 64 implementations of Seq. | |
| 65 | |
| 66 =head1 FEEDBACK | |
| 67 | |
| 68 =head2 Mailing Lists | |
| 69 | |
| 70 User feedback is an integral part of the evolution of this and other | |
| 71 Bioperl modules. Send your comments and suggestions preferably to one | |
| 72 of the Bioperl mailing lists. Your participation is much appreciated. | |
| 73 | |
| 74 bioperl-l@bioperl.org - General discussion | |
| 75 http://bio.perl.org/MailList.html - About the mailing lists | |
| 76 | |
| 77 =head2 Reporting Bugs | |
| 78 | |
| 79 Report bugs to the Bioperl bug tracking system to help us keep track | |
| 80 the bugs and their resolution. Bug reports can be submitted via email | |
| 81 or the web: | |
| 82 | |
| 83 bioperl-bugs@bio.perl.org | |
| 84 http://bugzilla.bioperl.org/ | |
| 85 | |
| 86 =head1 AUTHOR - Ewan Birney | |
| 87 | |
| 88 Email birney@sanger.ac.uk | |
| 89 | |
| 90 | |
| 91 =head1 APPENDIX | |
| 92 | |
| 93 The rest of the documentation details each of the object | |
| 94 methods. Internal methods are usually preceded with a _ | |
| 95 | |
| 96 =cut | |
| 97 | |
| 98 #' | |
| 99 # Let the code begin... | |
| 100 | |
| 101 | |
| 102 package Bio::SeqI; | |
| 103 use strict; | |
| 104 | |
| 105 use vars qw(@ISA); | |
| 106 use Bio::PrimarySeqI; | |
| 107 use Bio::AnnotatableI; | |
| 108 use Bio::FeatureHolderI; | |
| 109 | |
| 110 # Object preamble - inheriets from Bio::PrimarySeqI | |
| 111 | |
| 112 @ISA = qw(Bio::PrimarySeqI Bio::AnnotatableI Bio::FeatureHolderI); | |
| 113 | |
| 114 =head2 get_SeqFeatures | |
| 115 | |
| 116 Title : get_SeqFeatures | |
| 117 Usage : my @feats = $seq->get_SeqFeatures(); | |
| 118 Function: retrieve just the toplevel sequence features attached to this seq | |
| 119 Returns : array of Bio::SeqFeatureI objects | |
| 120 Args : none | |
| 121 | |
| 122 This method comes through extension of Bio::FeatureHolderI. See | |
| 123 L<Bio::FeatureHolderI> and L<Bio::SeqFeatureI> for more information. | |
| 124 | |
| 125 =cut | |
| 126 | |
| 127 =head2 get_all_SeqFeatures | |
| 128 | |
| 129 Title : get_all_SeqFeatures | |
| 130 Usage : @features = $annseq->get_all_SeqFeatures() | |
| 131 Function: returns all SeqFeatures, included sub SeqFeatures | |
| 132 Returns : an array of Bio::SeqFeatureI objects | |
| 133 Args : none | |
| 134 | |
| 135 This method comes through extension of Bio::FeatureHolderI. See | |
| 136 L<Bio::FeatureHolderI> and L<Bio::SeqFeatureI> for more information. | |
| 137 | |
| 138 =cut | |
| 139 | |
| 140 =head2 feature_count | |
| 141 | |
| 142 Title : feature_count | |
| 143 Usage : $seq->feature_count() | |
| 144 Function: Return the number of SeqFeatures attached to a sequence | |
| 145 Returns : integer representing the number of SeqFeatures | |
| 146 Args : none | |
| 147 | |
| 148 This method comes through extension of Bio::FeatureHolderI. See | |
| 149 L<Bio::FeatureHolderI> for more information. | |
| 150 | |
| 151 =cut | |
| 152 | |
| 153 =head2 seq | |
| 154 | |
| 155 Title : seq | |
| 156 Usage : my $string = $seq->seq(); | |
| 157 Function: Retrieves the sequence string for the sequence object | |
| 158 Returns : string | |
| 159 Args : none | |
| 160 | |
| 161 | |
| 162 =cut | |
| 163 | |
| 164 sub seq{ | |
| 165 my ($self) = @_; | |
| 166 $self->throw_not_implemented(); | |
| 167 } | |
| 168 | |
| 169 =head2 write_GFF | |
| 170 | |
| 171 Title : write_GFF | |
| 172 Usage : $seq->write_GFF(\*FILEHANDLE); | |
| 173 Function: Convience method to write out all the sequence features | |
| 174 in GFF format to the provided filehandle (STDOUT by default) | |
| 175 Returns : none | |
| 176 Args : [optional] filehandle to write to (default is STDOUT) | |
| 177 | |
| 178 | |
| 179 =cut | |
| 180 | |
| 181 sub write_GFF{ | |
| 182 my ($self,$fh) = @_; | |
| 183 | |
| 184 $fh || do { $fh = \*STDOUT; }; | |
| 185 | |
| 186 foreach my $sf ( $self->get_all_SeqFeatures() ) { | |
| 187 print $fh $sf->gff_string, "\n"; | |
| 188 } | |
| 189 | |
| 190 } | |
| 191 | |
| 192 =head2 annotation | |
| 193 | |
| 194 Title : annotation | |
| 195 Usage : $obj->annotation($seq_obj) | |
| 196 Function: retrieve the attached annotation object | |
| 197 Returns : Bio::AnnotationCollectionI or none; | |
| 198 | |
| 199 See L<Bio::AnnotationCollectionI> and L<Bio::Annotation::Collection> | |
| 200 for more information. This method comes through extension from | |
| 201 L<Bio::AnnotatableI>. | |
| 202 | |
| 203 =cut | |
| 204 | |
| 205 =head2 species | |
| 206 | |
| 207 Title : species | |
| 208 Usage : | |
| 209 Function: Gets or sets the species | |
| 210 Example : $species = $self->species(); | |
| 211 Returns : Bio::Species object | |
| 212 Args : Bio::Species object or none; | |
| 213 | |
| 214 See L<Bio::Species> for more information | |
| 215 | |
| 216 =cut | |
| 217 | |
| 218 sub species { | |
| 219 my ($self) = @_; | |
| 220 $self->throw_not_implemented(); | |
| 221 } | |
| 222 | |
| 223 =head2 primary_seq | |
| 224 | |
| 225 Title : primary_seq | |
| 226 Usage : $obj->primary_seq($newval) | |
| 227 Function: Retrieve the underlying Bio::PrimarySeqI object if available. | |
| 228 This is in the event one has a sequence with lots of features | |
| 229 but want to be able to narrow the object to just one with | |
| 230 the basics of a sequence (no features or annotations). | |
| 231 Returns : Bio::PrimarySeqI | |
| 232 Args : Bio::PrimarySeqI or none; | |
| 233 | |
| 234 See L<Bio::PrimarySeqI> for more information | |
| 235 | |
| 236 =cut | |
| 237 | |
| 238 sub primary_seq { | |
| 239 my ($self) = @_; | |
| 240 $self->throw_not_implemented; | |
| 241 } | |
| 242 | |
| 243 1; |
