Mercurial > repos > mahtabm > ensembl
comparison variant_effect_predictor/Bio/Index/Fasta.pm @ 0:1f6dce3d34e0
Uploaded
author | mahtabm |
---|---|
date | Thu, 11 Apr 2013 02:01:53 -0400 |
parents | |
children |
comparison
equal
deleted
inserted
replaced
-1:000000000000 | 0:1f6dce3d34e0 |
---|---|
1 # | |
2 # $Id: Fasta.pm,v 1.20 2002/10/22 07:38:33 lapp Exp $ | |
3 # | |
4 # BioPerl module for Bio::Index::Fasta | |
5 # | |
6 # Cared for by James Gilbert <jgrg@sanger.ac.uk> | |
7 # | |
8 # You may distribute this module under the same terms as perl itself | |
9 | |
10 # POD documentation - main docs before the code | |
11 | |
12 =head1 NAME | |
13 | |
14 Bio::Index::Fasta - Interface for indexing (multiple) fasta files | |
15 | |
16 =head1 SYNOPSIS | |
17 | |
18 # Complete code for making an index for several | |
19 # fasta files | |
20 use Bio::Index::Fasta; | |
21 use strict; | |
22 | |
23 my $Index_File_Name = shift; | |
24 my $inx = Bio::Index::Fasta->new( | |
25 '-filename' => $Index_File_Name, | |
26 '-write_flag' => 1); | |
27 $inx->make_index(@ARGV); | |
28 | |
29 # Print out several sequences present in the index | |
30 # in Fasta format | |
31 use Bio::Index::Fasta; | |
32 use strict; | |
33 | |
34 my $Index_File_Name = shift; | |
35 my $inx = Bio::Index::Fasta->new('-filename' => $Index_File_Name); | |
36 my $out = Bio::SeqIO->new('-format' => 'Fasta','-fh' => \*STDOUT); | |
37 | |
38 foreach my $id (@ARGV) { | |
39 my $seq = $inx->fetch($id); # Returns Bio::Seq object | |
40 $out->write_seq($seq); | |
41 } | |
42 | |
43 # or, alternatively | |
44 | |
45 my $seq = $inx->get_Seq_by_id($id); #identical to fetch | |
46 | |
47 =head1 DESCRIPTION | |
48 | |
49 Inherits functions for managing dbm files from Bio::Index::Abstract.pm, | |
50 and provides the basic funtionallity for indexing fasta files, and | |
51 retrieving the sequence from them. Note: for best results 'use strict'. | |
52 | |
53 Bio::Index::Fasta supports the Bio::DB::BioSeqI interface, meaning | |
54 it can be used a a Sequence database for other parts of bioperl | |
55 | |
56 =head1 FEED_BACK | |
57 | |
58 =head2 Mailing Lists | |
59 | |
60 User feedback is an integral part of the evolution of this and other | |
61 Bioperl modules. Send your comments and suggestions preferably to one | |
62 of the Bioperl mailing lists. Your participation is much appreciated. | |
63 | |
64 bioperl-l@bioperl.org - General discussion | |
65 http://bioperl.org/MailList.shtml - About the mailing lists | |
66 | |
67 =head2 Reporting Bugs | |
68 | |
69 Report bugs to the Bioperl bug tracking system to help us keep track | |
70 the bugs and their resolution. Bug reports can be submitted via | |
71 email or the web: | |
72 | |
73 bioperl-bugs@bio.perl.org | |
74 http://bugzilla.bioperl.org/ | |
75 | |
76 =head1 AUTHOR - James Gilbert | |
77 | |
78 Email - jgrg@sanger.ac.uk | |
79 | |
80 =head1 APPENDIX | |
81 | |
82 The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ | |
83 | |
84 =cut | |
85 | |
86 | |
87 # Let the code begin... | |
88 | |
89 | |
90 package Bio::Index::Fasta; | |
91 | |
92 use vars qw($VERSION @ISA); | |
93 use strict; | |
94 | |
95 use Bio::Index::AbstractSeq; | |
96 use Bio::Seq; | |
97 | |
98 @ISA = qw(Bio::Index::AbstractSeq); | |
99 | |
100 # | |
101 # Suggested fix by Michael G Schwern <schwern@pobox.com> to | |
102 # get around a clash with CPAN shell... | |
103 # | |
104 | |
105 BEGIN { | |
106 $VERSION = 0.2; | |
107 } | |
108 | |
109 sub _version { | |
110 return $VERSION; | |
111 } | |
112 | |
113 =head2 _file_format | |
114 | |
115 Title : _file_format | |
116 Function: The file format for this package, which is needed | |
117 by the SeqIO system when reading the sequence. | |
118 Returns : 'Fasta' | |
119 | |
120 =cut | |
121 | |
122 sub _file_format { | |
123 return 'Fasta'; | |
124 } | |
125 | |
126 | |
127 | |
128 =head2 _index_file | |
129 | |
130 Title : _index_file | |
131 Usage : $index->_index_file( $file_name, $i ) | |
132 Function: Specialist function to index FASTA format files. | |
133 Is provided with a filename and an integer | |
134 by make_index in its SUPER class. | |
135 Example : | |
136 Returns : | |
137 Args : | |
138 | |
139 =cut | |
140 | |
141 sub _index_file { | |
142 my( $self, | |
143 $file, # File name | |
144 $i, # Index-number of file being indexed | |
145 ) = @_; | |
146 | |
147 my( $begin, # Offset from start of file of the start | |
148 # of the last found record. | |
149 ); | |
150 | |
151 $begin = 0; | |
152 | |
153 my $id_parser = $self->id_parser; | |
154 | |
155 open FASTA, $file or $self->throw("Can't open file for read : $file"); | |
156 | |
157 # Main indexing loop | |
158 while (<FASTA>) { | |
159 if (/^>/) { | |
160 # $begin is the position of the first character after the '>' | |
161 my $begin = tell(FASTA) - length( $_ ) + 1; | |
162 | |
163 foreach my $id (&$id_parser($_)) { | |
164 $self->add_record($id, $i, $begin); | |
165 } | |
166 } | |
167 } | |
168 | |
169 close FASTA; | |
170 return 1; | |
171 } | |
172 | |
173 =head2 id_parser | |
174 | |
175 Title : id_parser | |
176 Usage : $index->id_parser( CODE ) | |
177 Function: Stores or returns the code used by record_id to | |
178 parse the ID for record from a string. Useful | |
179 for (for instance) specifying a different | |
180 parser for different flavours of FASTA file. | |
181 Returns \&default_id_parser (see below) if not | |
182 set. If you supply your own id_parser | |
183 subroutine, then it should expect a fasta | |
184 description line. An entry will be added to | |
185 the index for each string in the list returned. | |
186 Example : $index->id_parser( \&my_id_parser ) | |
187 Returns : ref to CODE if called without arguments | |
188 Args : CODE | |
189 | |
190 =cut | |
191 | |
192 sub id_parser { | |
193 my( $self, $code ) = @_; | |
194 | |
195 if ($code) { | |
196 $self->{'_id_parser'} = $code; | |
197 } | |
198 return $self->{'_id_parser'} || \&default_id_parser; | |
199 } | |
200 | |
201 | |
202 | |
203 =head2 default_id_parser | |
204 | |
205 Title : default_id_parser | |
206 Usage : $id = default_id_parser( $header ) | |
207 Function: The default Fasta ID parser for Fasta.pm | |
208 Returns $1 from applying the regexp /^>\s*(\S+)/ | |
209 to $header. | |
210 Returns : ID string | |
211 Args : a fasta header line string | |
212 | |
213 =cut | |
214 | |
215 sub default_id_parser { | |
216 if ($_[0] =~ /^>\s*(\S+)/) { | |
217 return $1; | |
218 } else { | |
219 return; | |
220 } | |
221 } | |
222 | |
223 1; |