Mercurial > repos > mahtabm > ensembl
comparison variant_effect_predictor/Bio/Index/Fasta.pm @ 0:1f6dce3d34e0
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| author | mahtabm |
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| date | Thu, 11 Apr 2013 02:01:53 -0400 |
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| -1:000000000000 | 0:1f6dce3d34e0 |
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| 1 # | |
| 2 # $Id: Fasta.pm,v 1.20 2002/10/22 07:38:33 lapp Exp $ | |
| 3 # | |
| 4 # BioPerl module for Bio::Index::Fasta | |
| 5 # | |
| 6 # Cared for by James Gilbert <jgrg@sanger.ac.uk> | |
| 7 # | |
| 8 # You may distribute this module under the same terms as perl itself | |
| 9 | |
| 10 # POD documentation - main docs before the code | |
| 11 | |
| 12 =head1 NAME | |
| 13 | |
| 14 Bio::Index::Fasta - Interface for indexing (multiple) fasta files | |
| 15 | |
| 16 =head1 SYNOPSIS | |
| 17 | |
| 18 # Complete code for making an index for several | |
| 19 # fasta files | |
| 20 use Bio::Index::Fasta; | |
| 21 use strict; | |
| 22 | |
| 23 my $Index_File_Name = shift; | |
| 24 my $inx = Bio::Index::Fasta->new( | |
| 25 '-filename' => $Index_File_Name, | |
| 26 '-write_flag' => 1); | |
| 27 $inx->make_index(@ARGV); | |
| 28 | |
| 29 # Print out several sequences present in the index | |
| 30 # in Fasta format | |
| 31 use Bio::Index::Fasta; | |
| 32 use strict; | |
| 33 | |
| 34 my $Index_File_Name = shift; | |
| 35 my $inx = Bio::Index::Fasta->new('-filename' => $Index_File_Name); | |
| 36 my $out = Bio::SeqIO->new('-format' => 'Fasta','-fh' => \*STDOUT); | |
| 37 | |
| 38 foreach my $id (@ARGV) { | |
| 39 my $seq = $inx->fetch($id); # Returns Bio::Seq object | |
| 40 $out->write_seq($seq); | |
| 41 } | |
| 42 | |
| 43 # or, alternatively | |
| 44 | |
| 45 my $seq = $inx->get_Seq_by_id($id); #identical to fetch | |
| 46 | |
| 47 =head1 DESCRIPTION | |
| 48 | |
| 49 Inherits functions for managing dbm files from Bio::Index::Abstract.pm, | |
| 50 and provides the basic funtionallity for indexing fasta files, and | |
| 51 retrieving the sequence from them. Note: for best results 'use strict'. | |
| 52 | |
| 53 Bio::Index::Fasta supports the Bio::DB::BioSeqI interface, meaning | |
| 54 it can be used a a Sequence database for other parts of bioperl | |
| 55 | |
| 56 =head1 FEED_BACK | |
| 57 | |
| 58 =head2 Mailing Lists | |
| 59 | |
| 60 User feedback is an integral part of the evolution of this and other | |
| 61 Bioperl modules. Send your comments and suggestions preferably to one | |
| 62 of the Bioperl mailing lists. Your participation is much appreciated. | |
| 63 | |
| 64 bioperl-l@bioperl.org - General discussion | |
| 65 http://bioperl.org/MailList.shtml - About the mailing lists | |
| 66 | |
| 67 =head2 Reporting Bugs | |
| 68 | |
| 69 Report bugs to the Bioperl bug tracking system to help us keep track | |
| 70 the bugs and their resolution. Bug reports can be submitted via | |
| 71 email or the web: | |
| 72 | |
| 73 bioperl-bugs@bio.perl.org | |
| 74 http://bugzilla.bioperl.org/ | |
| 75 | |
| 76 =head1 AUTHOR - James Gilbert | |
| 77 | |
| 78 Email - jgrg@sanger.ac.uk | |
| 79 | |
| 80 =head1 APPENDIX | |
| 81 | |
| 82 The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ | |
| 83 | |
| 84 =cut | |
| 85 | |
| 86 | |
| 87 # Let the code begin... | |
| 88 | |
| 89 | |
| 90 package Bio::Index::Fasta; | |
| 91 | |
| 92 use vars qw($VERSION @ISA); | |
| 93 use strict; | |
| 94 | |
| 95 use Bio::Index::AbstractSeq; | |
| 96 use Bio::Seq; | |
| 97 | |
| 98 @ISA = qw(Bio::Index::AbstractSeq); | |
| 99 | |
| 100 # | |
| 101 # Suggested fix by Michael G Schwern <schwern@pobox.com> to | |
| 102 # get around a clash with CPAN shell... | |
| 103 # | |
| 104 | |
| 105 BEGIN { | |
| 106 $VERSION = 0.2; | |
| 107 } | |
| 108 | |
| 109 sub _version { | |
| 110 return $VERSION; | |
| 111 } | |
| 112 | |
| 113 =head2 _file_format | |
| 114 | |
| 115 Title : _file_format | |
| 116 Function: The file format for this package, which is needed | |
| 117 by the SeqIO system when reading the sequence. | |
| 118 Returns : 'Fasta' | |
| 119 | |
| 120 =cut | |
| 121 | |
| 122 sub _file_format { | |
| 123 return 'Fasta'; | |
| 124 } | |
| 125 | |
| 126 | |
| 127 | |
| 128 =head2 _index_file | |
| 129 | |
| 130 Title : _index_file | |
| 131 Usage : $index->_index_file( $file_name, $i ) | |
| 132 Function: Specialist function to index FASTA format files. | |
| 133 Is provided with a filename and an integer | |
| 134 by make_index in its SUPER class. | |
| 135 Example : | |
| 136 Returns : | |
| 137 Args : | |
| 138 | |
| 139 =cut | |
| 140 | |
| 141 sub _index_file { | |
| 142 my( $self, | |
| 143 $file, # File name | |
| 144 $i, # Index-number of file being indexed | |
| 145 ) = @_; | |
| 146 | |
| 147 my( $begin, # Offset from start of file of the start | |
| 148 # of the last found record. | |
| 149 ); | |
| 150 | |
| 151 $begin = 0; | |
| 152 | |
| 153 my $id_parser = $self->id_parser; | |
| 154 | |
| 155 open FASTA, $file or $self->throw("Can't open file for read : $file"); | |
| 156 | |
| 157 # Main indexing loop | |
| 158 while (<FASTA>) { | |
| 159 if (/^>/) { | |
| 160 # $begin is the position of the first character after the '>' | |
| 161 my $begin = tell(FASTA) - length( $_ ) + 1; | |
| 162 | |
| 163 foreach my $id (&$id_parser($_)) { | |
| 164 $self->add_record($id, $i, $begin); | |
| 165 } | |
| 166 } | |
| 167 } | |
| 168 | |
| 169 close FASTA; | |
| 170 return 1; | |
| 171 } | |
| 172 | |
| 173 =head2 id_parser | |
| 174 | |
| 175 Title : id_parser | |
| 176 Usage : $index->id_parser( CODE ) | |
| 177 Function: Stores or returns the code used by record_id to | |
| 178 parse the ID for record from a string. Useful | |
| 179 for (for instance) specifying a different | |
| 180 parser for different flavours of FASTA file. | |
| 181 Returns \&default_id_parser (see below) if not | |
| 182 set. If you supply your own id_parser | |
| 183 subroutine, then it should expect a fasta | |
| 184 description line. An entry will be added to | |
| 185 the index for each string in the list returned. | |
| 186 Example : $index->id_parser( \&my_id_parser ) | |
| 187 Returns : ref to CODE if called without arguments | |
| 188 Args : CODE | |
| 189 | |
| 190 =cut | |
| 191 | |
| 192 sub id_parser { | |
| 193 my( $self, $code ) = @_; | |
| 194 | |
| 195 if ($code) { | |
| 196 $self->{'_id_parser'} = $code; | |
| 197 } | |
| 198 return $self->{'_id_parser'} || \&default_id_parser; | |
| 199 } | |
| 200 | |
| 201 | |
| 202 | |
| 203 =head2 default_id_parser | |
| 204 | |
| 205 Title : default_id_parser | |
| 206 Usage : $id = default_id_parser( $header ) | |
| 207 Function: The default Fasta ID parser for Fasta.pm | |
| 208 Returns $1 from applying the regexp /^>\s*(\S+)/ | |
| 209 to $header. | |
| 210 Returns : ID string | |
| 211 Args : a fasta header line string | |
| 212 | |
| 213 =cut | |
| 214 | |
| 215 sub default_id_parser { | |
| 216 if ($_[0] =~ /^>\s*(\S+)/) { | |
| 217 return $1; | |
| 218 } else { | |
| 219 return; | |
| 220 } | |
| 221 } | |
| 222 | |
| 223 1; |
