comparison variant_effect_predictor/Bio/EnsEMBL/IdMapping/Archiver.pm @ 0:1f6dce3d34e0

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=head1 LICENSE

  Copyright (c) 1999-2012 The European Bioinformatics Institute and
  Genome Research Limited.  All rights reserved.

  This software is distributed under a modified Apache license.
  For license details, please see

    http://www.ensembl.org/info/about/code_licence.html

=head1 CONTACT

  Please email comments or questions to the public Ensembl
  developers list at <[e-mail]>.

  Questions may also be sent to the Ensembl help desk at
  <[e-mail]>.

=cut

=head1 NAME

Bio::EnsEMBL::IdMapping::Archiver - create gene_archive and peptide_archive

=head1 SYNOPSIS

  my $archiver = Bio::EnsEMBL::IdMapping::Archiver->new(
    -LOGGER => $logger,
    -CONF   => $conf,
    -CACHE  => $cache
  );

  # create gene and peptide archive
  $archiver->create_archive($mapping_session_id);

  # dump existing archive tables to file
  my $num_entries =
    $archiver->dump_table_to_file( 'source', 'gene_archive',
    'gene_archive_existing.txt', 1 );

=head1 DESCRIPTION

This module creates the gene_archive and peptide_archive
tables. Data is written to a file as tab-delimited text for
loading into a MySQL database (this can be done manually, or using
StableIdmapper->upload_file_into_table()).

An archive entry for a given source gene is created if no target
gene exists, or if any of its transcripts or their translations
changed. Non-coding transcripts only have an entry in gene_archive (i.e.
without a corresponding peptide_archive entry).

=head1 METHODS

  create_archive
  dump_gene
  dump_tuple
  dump_nc_row
  mapping_session_id

=cut


package Bio::EnsEMBL::IdMapping::Archiver;

use strict;
use warnings;
no warnings 'uninitialized';

use Bio::EnsEMBL::IdMapping::BaseObject;
our @ISA = qw(Bio::EnsEMBL::IdMapping::BaseObject);

use Bio::EnsEMBL::Utils::Exception qw(throw warning);
use Bio::EnsEMBL::Utils::ScriptUtils qw(path_append);
use Digest::MD5 qw(md5_hex);


# instance variables
my $pa_id;


=head2 create_archive

  Arg[1]      : Int $mapping_session_id - the mapping_session_id for this run
  Example     : $archiver->create_archive($stable_id_mapper->mapping_session_id);
  Description : Creates the gene_archive and peptide_archive tables and writes
                the data to a tab-delimited file. The decision as to what to
                archive is deferred to dump_gene(), see documentation there for
                details.
  Return type : none
  Exceptions  : Thrown on missing argument.
  Caller      : id_mapping.pl
  Status      : At Risk
              : under development

=cut

sub create_archive {
  my $self = shift;
  my $mapping_session_id = shift;

  # argument check
  unless ($mapping_session_id) {
    $self->logger->warning("No mapping_session_id set.");
  }

  $self->mapping_session_id($mapping_session_id);

  # get filehandles to write gene and peptide archive
  my $ga_fh = $self->get_filehandle('gene_archive_new.txt', 'tables');
  my $pa_fh = $self->get_filehandle('peptide_archive_new.txt', 'tables');

  # get the currently highest peptide_archive_id from the source db
  my $s_dba = $self->cache->get_DBAdaptor('source');
  my $s_dbh = $s_dba->dbc->db_handle;
  my $sql = qq(SELECT MAX(peptide_archive_id) FROM peptide_archive);
  $pa_id = $self->fetch_value_from_db($s_dbh, $sql);

  unless ($pa_id) {
    $self->logger->warning("No max(peptide_archive_id) found in db.\n", 1);
    $self->logger->info("That's ok if this is the first stable ID mapping for this species.\n", 1);
  }
  
  $pa_id++;
  $self->logger->debug("Starting with peptide_archive_id $pa_id.\n");

  # lookup hash of target gene stable IDs
  my %target_genes = map { $_->stable_id => $_ }
    values %{ $self->cache->get_by_name("genes_by_id", 'target') };

  # loop over source genes and dump to archive (dump_gene() will decide whether
  # to do full or partial dump)
  foreach my $source_gene (values %{ $self->cache->get_by_name("genes_by_id",
    'source') }) {

    $self->dump_gene($source_gene, $target_genes{$source_gene->stable_id}, 
      $ga_fh, $pa_fh);
  }
  
  close($ga_fh);
  close($pa_fh);
}


=head2 dump_gene

  Arg[1]      : Bio::EnsEMBL::IdMapping::TinyGene $s_gene - source gene
  Arg[2]      : Bio::EnsEMBL::IdMapping::TinyGene $t_gene - target gene
  Arg[3]      : Filehandle $ga_fh - filehandle for writing gene_archive data
  Arg[4]      : Filehandle $pa_fh - filehandle for writing peptide_archive data
  Example     : my $target_gene = $gene_mappings{$source_gene->stable_id};
                $archiver->dump_gene($source_gene, $target_gene, $ga_fh, $pa_fh);
  Description : Given a source gene, it will write a gene_achive and
                peptide_achive entry for it if no target gene exists, or if any
                of its transcripts or their translation changed. 
  Return type : none
  Exceptions  : none
  Caller      : create_archive()
  Status      : At Risk
              : under development

=cut

sub dump_gene {
  my ($self, $s_gene, $t_gene, $ga_fh, $pa_fh) = @_;

  # private method, so no argument check done for performance reasons
  
  # deal with ncRNA differently
  # hope this simple biotype regex is accurate enough...
  my $is_ncRNA = 0;
  $is_ncRNA = 1 if ($s_gene->biotype =~ /RNA/);

  # loop over source transcripts
  foreach my $s_tr (@{ $s_gene->get_all_Transcripts }) {
    my $s_tl = $s_tr->translation;

    # we got a coding transcript
    if ($s_tl) {
      
      # do a full dump of this gene if no target gene exists
      if (! $t_gene) {
        $self->dump_tuple($s_gene, $s_tr, $s_tl, $ga_fh, $pa_fh);

      # otherwise, only dump if any transcript or its translation changed
      } else {

        my $changed_flag = 1;
      
        foreach my $t_tr (@{ $t_gene->get_all_Transcripts }) {
          my $t_tl = $t_tr->translation;
          next unless ($t_tl);

          if (($s_tr->stable_id eq $t_tr->stable_id) and
              ($s_tl->stable_id eq $t_tl->stable_id) and
              ($s_tl->seq eq $t_tl->seq)) {
            $changed_flag = 0;
          }
        }

        if ($changed_flag) {
          $self->dump_tuple($s_gene, $s_tr, $s_tl, $ga_fh, $pa_fh);
        }
      }

    # now deal with ncRNAs (they don't translate but we still want to archive
    # them)
    } elsif ($is_ncRNA) {
    
      if (! $t_gene) {
    
        $self->dump_nc_row($s_gene, $s_tr, $ga_fh);
    
      } else {
        
        my $changed_flag = 1;
      
        foreach my $t_tr (@{ $t_gene->get_all_Transcripts }) {
          $changed_flag = 0 if ($s_tr->stable_id eq $t_tr->stable_id);
        }

        if ($changed_flag) {
          $self->dump_nc_row($s_gene, $s_tr, $ga_fh);
        }
      
      }
    }
  }
}


=head2 dump_tuple

  Arg[1]      : Bio::EnsEMBL::IdMapping::TinyGene $gene - gene to archive
  Arg[2]      : Bio::EnsEMBL::IdMapping::TinyTrancript $tr - its transcript
  Arg[3]      : Bio::EnsEMBL::IdMapping::TinyTranslation $tl - its translation
  Arg[4]      : Filehandle $ga_fh - filehandle for writing gene_archive data
  Arg[5]      : Filehandle $pa_fh - filehandle for writing peptide_archive data
  Example     : $archive->dump_tuple($s_gene, $s_tr, $s_tl, $ga_fh, $pa_fh);
  Description : Writes entry lines for gene_archive and peptide_archive.
  Return type : none
  Exceptions  : none
  Caller      : dump_gene()
  Status      : At Risk
              : under development

=cut

sub dump_tuple {
  my ($self, $gene, $tr, $tl, $ga_fh, $pa_fh) = @_;
  
  # private method, so no argument check done for performance reasons

  # gene archive
  print $ga_fh join("\t",
                    $gene->stable_id,
                    $gene->version,
                    $tr->stable_id,
                    $tr->version,
                    $tl->stable_id,
                    $tl->version,
                    $pa_id,
                    $self->mapping_session_id
  );
  print $ga_fh "\n";

  # peptide archive
  my $pep_seq = $tl->seq;
  print $pa_fh join("\t", $pa_id, md5_hex($pep_seq), $pep_seq);
  print $pa_fh "\n";

  # increment peptide_archive_id
  $pa_id++;
}


=head2 dump_nc_row

  Arg[1]      : Bio::EnsEMBL::IdMapping::TinyGene $gene - gene to archive
  Arg[2]      : Bio::EnsEMBL::IdMapping::TinyTrancript $tr - its transcript
  Arg[3]      : Filehandle $ga_fh - filehandle for writing gene_archive data
  Example     : $archive->dump_nc_row($s_gene, $s_tr, $ga_fh);
  Description : Writes an entry line for gene_archive for non-coding
                transcripts.
  Return type : none
  Exceptions  : none
  Caller      : dump_gene()
  Status      : At Risk
              : under development

=cut

sub dump_nc_row {
  my ($self, $gene, $tr, $ga_fh) = @_;
  
  # private method, so no argument check done for performance reasons

  # gene archive
  print $ga_fh join("\t",
                    $gene->stable_id,
                    $gene->version,
                    $tr->stable_id,
                    $tr->version,
                    '\N',
                    '\N',
                    '\N',
                    $self->mapping_session_id
  );
  print $ga_fh "\n";
}


=head2 mapping_session_id

  Arg[1]      : (optional) Int - mapping_session_id to set
  Example     : my $msi = $archiver->mapping_session_id;
  Description : Getter/setter for mapping_session_id.
  Return type : Int
  Exceptions  : none
  Caller      : create_archive()
  Status      : At Risk
              : under development

=cut

sub mapping_session_id {
  my $self = shift;
  $self->{'_mapping_session_id'} = shift if (@_);
  return $self->{'_mapping_session_id'};
}


1;