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1 =head1 LICENSE
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2
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3 Copyright (c) 1999-2012 The European Bioinformatics Institute and
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4 Genome Research Limited. All rights reserved.
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5
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6 This software is distributed under a modified Apache license.
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7 For license details, please see
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8
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9 http://www.ensembl.org/info/about/code_licence.html
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10
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11 =head1 CONTACT
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12
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13 Please email comments or questions to the public Ensembl
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14 developers list at <dev@ensembl.org>.
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15
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16 Questions may also be sent to the Ensembl help desk at
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17 <helpdesk@ensembl.org>.
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18
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19 =cut
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20
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21 =head1 NAME
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22
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23 Bio::EnsEMBL::DBSQL::MiscFeatureAdaptor
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24
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25 =head1 SYNOPSIS
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26
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27 $mfa = $database_adaptor->get_MiscFeatureAdaptor();
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28
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29 # retrieve a misc feature by its dbID
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30 my $misc_feat = $mfa->fetch_by_dbID(1234);
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31
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32 # retrieve all misc features in a given region
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33 my @misc_feats = @{ $mfa->fetch_all_by_Slice($slice) };
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34
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35 # retrieve all misc features in a given region with a given set code
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36 my @misc_clones =
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37 @{ $mfa->fetch_all_by_Slice_and_set_code('cloneset') };
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38
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39 # store some misc features in the database
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40 $mfa->store(@misc_features);
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41
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42 =head1 DESCRIPTION
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43
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44 This is an adaptor for the retrieval and storage of MiscFeatures.
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45 Misc Features are extremely generic features that can be added with
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46 minimal effort to the database. Currently misc features are used to
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47 describe the locations of clone sets and tiling path information,
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48 but arbitrary features can be stored. Misc features are grouped
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49 into sets and can be fetched according to their grouping using the
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50 fetch_all_by_Slice_and_set_code and fetch_all_by_set_code methods.
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51 MiscFeatures may belong to more than one set.
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52
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53 =head1 METHODS
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54
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55 =cut
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56
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57 package Bio::EnsEMBL::DBSQL::MiscFeatureAdaptor;
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58
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59 use strict;
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60 use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
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61 use Bio::EnsEMBL::MiscFeature;
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62 use Bio::EnsEMBL::Attribute;
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63 use Bio::EnsEMBL::MiscSet;
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64 use Bio::EnsEMBL::Utils::Exception qw(throw warning);
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65
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66 use vars qw(@ISA);
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67
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68 @ISA = qw(Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor);
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69
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70
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71
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72 =head2 fetch_all_by_Slice_and_set_code
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73
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74 Arg [1] : Bio::EnsEMBL::Slice $slice
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75 A slice representing the region to fetch from
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76 Arg [2...] : string $set_code
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77 The code of the set to retrieve features from
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78 Example : @feats = @{$mfa->fetch_all_by_Slice_and_set_code('cloneset')};
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79 Description: Retrieves a set of MiscFeatures which have a particular set code
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80 and which lie in a particular region. All features with the
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81 provide set code and which overlap the given slice are returned.
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82 Returntype : listref of Bio::EnsEMBL::MiscFeatures
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83 Exceptions : throw if set_code is not provided
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84 warning if no set for provided set code exists
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85 Caller : general
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86 Status : Stable
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87
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88 =cut
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89
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90 sub fetch_all_by_Slice_and_set_code {
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91 my $self = shift;
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92 my $slice = shift;
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93
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94 throw('Set code argument is required.') unless @_;
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95
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96 my $msa = $self->db->get_MiscSetAdaptor();
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97 my @sets = ();
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98 my $max_len = 0;
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99 foreach my $set_code (@_) {
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100 my $set = $msa->fetch_by_code($set_code);
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101 if($set) {
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102 $max_len = $set->longest_feature if $set->longest_feature > $max_len;
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103 push @sets, $set->dbID;
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104 } else {
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105 warning("No misc_set with code [$set_code] exists");
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106 }
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107 }
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108 my $constraint;
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109 if( @sets > 1 ) {
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110 $constraint = " mfms.misc_set_id in ( @{[join ',',@sets]} ) ";
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111 } elsif( @sets == 1 ) {
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112 $constraint = " mfms.misc_set_id = $sets[0] ";
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113 } else {
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114 return [];
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115 }
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116
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117 $self->_max_feature_length($max_len);
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118
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119 my $results = $self->fetch_all_by_Slice_constraint($slice, $constraint);
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120
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121 $self->_max_feature_length(undef);
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122
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123 return $results;
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124 }
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125
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126
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127
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128 =head2 fetch_all_by_attribute_type_value
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129
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130 Arg [1] : string $attrib_type_code
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131 The code of the attribute type to fetch features for
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132 Arg [2] : (optional) string $attrib_value
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133 The value of the attribute to fetch features for
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134 Example :
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135 #get all misc features that have an embl accession
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136 @feats = @{$mfa->fetch_all_by_attrib_type_value('embl_acc')};
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137 #get the misc feature with synonym 'AL014121'
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138 ($feat)=@{$mfa->fetch_all_by_attrib_type_value('synonym','AL014121');
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139 Description: Retrieves MiscFeatures which have a particular attribute.
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140 If the attribute value argument is also provided only
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141 features which have the attribute AND a particular value
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142 are returned. The features are returned in their native
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143 coordinate system (i.e. the coordinate system that they
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144 are stored in).
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145 Returntype : listref of Bio::EnsEMBL::MiscFeatures
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146 Exceptions : throw if attrib_type code arg is not provided
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147 Caller : general
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148 Status : Stable
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149
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150 =cut
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151
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152 sub fetch_all_by_attribute_type_value {
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153 my $self = shift;
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154 my $attrib_type_code = shift;
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155 my $attrib_value = shift;
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156
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157 throw("Attrib type code argument is required.")
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158 if ( !$attrib_type_code );
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159
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160 # Need to do 2 queries so that all of the ids come back with the
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161 # features. The problem with adding attrib constraints to filter the
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162 # misc_features which come back is that not all of the attributes will
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163 # come back
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164
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165 my $sql = qq(
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166 SELECT DISTINCT
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167 ma.misc_feature_id
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168 FROM misc_attrib ma,
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169 attrib_type at,
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170 misc_feature mf,
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171 seq_region sr,
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172 coord_system cs
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173 WHERE ma.attrib_type_id = at.attrib_type_id
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174 AND at.code = ?
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175 AND ma.misc_feature_id = mf.misc_feature_id
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176 AND mf.seq_region_id = sr.seq_region_id
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177 AND sr.coord_system_id = cs.coord_system_id
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178 AND cs.species_id = ?);
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179
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180 if ($attrib_value) {
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181 $sql .= " AND ma.value = ?";
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182 }
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183
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184 my $sth = $self->prepare($sql);
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185
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186 $sth->bind_param( 1, $attrib_type_code, SQL_VARCHAR );
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187 $sth->bind_param( 2, $self->species_id(), SQL_INTEGER );
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188 if ($attrib_value) {
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189 $sth->bind_param( 3, $attrib_value, SQL_VARCHAR );
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190 }
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191
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192 $sth->execute();
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193
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194 my @ids = map { $_->[0] } @{ $sth->fetchall_arrayref() };
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195
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196 $sth->finish();
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197
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198 # Construct constraints from the list of ids. Split ids into groups
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199 # of 1000 to ensure that the query is not too big.
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200 my @constraints;
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201 while (@ids) {
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202 my @subset = splice( @ids, 0, 1000 );
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203 if ( @subset == 1 ) {
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204 push @constraints, "mf.misc_feature_id = $subset[0]";
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205 } else {
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206 my $id_str = join( ',', @subset );
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207 push @constraints, "mf.misc_feature_id in ($id_str)";
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208 }
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209 }
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210
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211 my @results;
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212 foreach my $constraint (@constraints) {
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213 push @results, @{ $self->generic_fetch($constraint) };
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214 }
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215
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216 return \@results;
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217 } ## end sub fetch_all_by_attribute_type_value
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218
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219
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220 #_tables
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221 #
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222 # Arg [1] : none
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223 # Example : none
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224 # Description: PROTECTED Implementation of abstract superclass method to
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225 # provide the name of the tables to query
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226 # Returntype : string
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227 # Exceptions : none
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228 # Caller : internal
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229
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230
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231 sub _tables {
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232 my $self = shift;
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233
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234 return (['misc_feature', 'mf'],
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235 ['misc_feature_misc_set', 'mfms'],
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236 ['misc_attrib', 'ma'],
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237 ['attrib_type', 'at']);
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238 }
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239
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240
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241 #_columns
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242
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243 # Arg [1] : none
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244 # Example : none
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245 # Description: PROTECTED Implementation of abstract superclass method to
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246 # provide the name of the columns to query
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247 # Returntype : list of strings
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248 # Exceptions : none
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249 # Caller : internal
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250
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251 sub _columns {
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252 my $self = shift;
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253
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254 #warning _objs_from_sth implementation depends on ordering
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255 return qw (mf.misc_feature_id
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256 mf.seq_region_id
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257 mf.seq_region_start
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258 mf.seq_region_end
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259 mf.seq_region_strand
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260 ma.value
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261 at.code
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262 mfms.misc_set_id
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263 at.name
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264 at.description);
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265 }
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266
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267
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268
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269 # _default_where_clause
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270
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271 # Arg [1] : none
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272 # Example : none
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273 # Description: Overrides superclass method to provide an additional
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274 # table joining constraint before the SQL query is performed.
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275 # Returntype : string
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276 # Exceptions : none
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277 # Caller : generic_fetch
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278
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279 sub _default_where_clause {
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280 my $self = shift;
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281
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282 return '';
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283 }
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284
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285
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286 sub _left_join {
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287 my $self = shift;
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288
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289 return(
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290 ['misc_feature_misc_set','mf.misc_feature_id = mfms.misc_feature_id'],
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291 ['misc_attrib', 'mf.misc_feature_id = ma.misc_feature_id'],
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292 ['attrib_type','ma.attrib_type_id = at.attrib_type_id']);
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293 }
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294
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295
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296 sub _final_clause {
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297 my $self = shift;
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298
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299 return " ORDER BY mf.misc_feature_id";
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300 }
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301
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302
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303 # _objs_from_sth
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304
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305 # Arg [1] : StatementHandle $sth
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306 # Example : none
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307 # Description: PROTECTED implementation of abstract superclass method.
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308 # responsible for the creation of MiscFeatures from a
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309 # hashref generated from an SQL query
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310 # Returntype : listref of Bio::EnsEMBL::MiscFeatures
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311 # Exceptions : none
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312 # Caller : internal
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313
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314 sub _objs_from_sth {
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315 my ($self, $sth, $mapper, $dest_slice) = @_;
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316
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317 #
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318 # This code is ugly because an attempt has been made to remove as many
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319 # function calls as possible for speed purposes. Thus many caches and
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320 # a fair bit of gymnastics is used.
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321 #
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322
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323 my $sa = $self->db()->get_SliceAdaptor();
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324 my $msa = $self->db->get_MiscSetAdaptor();
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325
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326 my @features;
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327 my %ms_hash;
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328 my %slice_hash;
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329 my %sr_name_hash;
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330 my %sr_cs_hash;
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331
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332 my($misc_feature_id, $seq_region_id, $seq_region_start, $seq_region_end,
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333 $seq_region_strand, $attrib_value, $attrib_type_code, $misc_set_id,
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334 $attrib_type_name, $attrib_type_description );
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335
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336 $sth->bind_columns( \$misc_feature_id, \$seq_region_id, \$seq_region_start,
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337 \$seq_region_end, \$seq_region_strand,
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338 \$attrib_value, \$attrib_type_code,\$misc_set_id,
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339 \$attrib_type_name, \$attrib_type_description );
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340
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341 my $asm_cs;
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342 my $cmp_cs;
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343 my $asm_cs_vers;
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344 my $asm_cs_name;
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345 my $cmp_cs_vers;
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346 my $cmp_cs_name;
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347 if($mapper) {
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348 $asm_cs = $mapper->assembled_CoordSystem();
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349 $cmp_cs = $mapper->component_CoordSystem();
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350 $asm_cs_name = $asm_cs->name();
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351 $asm_cs_vers = $asm_cs->version();
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352 $cmp_cs_name = $cmp_cs->name();
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353 $cmp_cs_vers = $cmp_cs->version();
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354 }
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355
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356 my $dest_slice_start;
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357 my $dest_slice_end;
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358 my $dest_slice_strand;
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359 my $dest_slice_length;
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360 my $dest_slice_sr_name;
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361 my $dest_slice_sr_id;
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362 if($dest_slice) {
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363 $dest_slice_start = $dest_slice->start();
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364 $dest_slice_end = $dest_slice->end();
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365 $dest_slice_strand = $dest_slice->strand();
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366 $dest_slice_length = $dest_slice->length();
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367 $dest_slice_sr_name = $dest_slice->seq_region_name();
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368 $dest_slice_sr_id = $dest_slice->get_seq_region_id();
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369 }
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370
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371 my $current = -1;
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372 my $throw_away = -1;
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373 my $feat;
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374 my $feat_misc_sets;
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375 my $feat_attribs;
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376 my $seen_attribs;
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377
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378 FEATURE: while($sth->fetch()) {
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379 #if this feature is not being used, skip all rows related to it
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380 next if($throw_away == $misc_feature_id);
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381
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382 if ($current == $misc_feature_id) {
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383 #still working on building up attributes and sets for current feature
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384
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385 #if there is a misc_set, add it to the current feature
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386 if ($misc_set_id) {
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387 my $misc_set = $ms_hash{$misc_set_id} ||=
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388 $msa->fetch_by_dbID($misc_set_id);
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389 if ( ! exists $feat_misc_sets->{$misc_set->{'code'}} ) {
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390 $feat->add_MiscSet( $misc_set );
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391 $feat_misc_sets->{$misc_set->{'code'}} = $misc_set;
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392 }
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393 }
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394
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395 #if there is a new attribute add it to the current feature
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396 if ($attrib_value && $attrib_type_code &&
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397 !$seen_attribs->{"$attrib_type_code:$attrib_value"}) {
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398 my $attrib = Bio::EnsEMBL::Attribute->new
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399 ( -CODE => $attrib_type_code,
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400 -NAME => $attrib_type_name,
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401 -DESC => $attrib_type_description,
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402 -VALUE => $attrib_value
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403 );
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404
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405
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406 $feat_attribs ||= [];
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407 push @$feat_attribs, $attrib;
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408 $seen_attribs->{"$attrib_type_code:$attrib_value"} = 1;
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409 }
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410
|
|
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411 } else {
|
|
|
412 if ($feat) {
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413 #start working on a new feature, discard references to last one
|
|
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414 $feat = {};
|
|
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415 $feat_attribs = [];
|
|
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416 $feat_misc_sets = {};
|
|
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417 $seen_attribs = {};
|
|
|
418 }
|
|
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419
|
|
|
420 $current = $misc_feature_id;
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|
421 #need to get the internal_seq_region, if present
|
|
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422 $seq_region_id = $self->get_seq_region_id_internal($seq_region_id);
|
|
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423 my $slice = $slice_hash{"ID:".$seq_region_id};
|
|
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424
|
|
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425 if (!$slice) {
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426 $slice = $sa->fetch_by_seq_region_id($seq_region_id);
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427 $slice_hash{"ID:".$seq_region_id} = $slice;
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428 $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
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429 $sr_cs_hash{$seq_region_id} = $slice->coord_system();
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|
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430 }
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|
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431
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|
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432 my $sr_name = $sr_name_hash{$seq_region_id};
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433 my $sr_cs = $sr_cs_hash{$seq_region_id};
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434 #
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|
|
435 # remap the feature coordinates to another coord system
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436 # if a mapper was provided
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437 #
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438 if ($mapper) {
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439
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440
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|
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441 if (defined $dest_slice && $mapper->isa('Bio::EnsEMBL::ChainedAssemblyMapper') ) {
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442 ( $seq_region_id, $seq_region_start,$seq_region_end, $seq_region_strand ) =
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443 $mapper->map( $sr_name, $seq_region_start, $seq_region_end,
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444 $seq_region_strand, $sr_cs, 1, $dest_slice);
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445
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446 } else {
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447
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|
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448 ( $seq_region_id, $seq_region_start,$seq_region_end, $seq_region_strand ) =
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|
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449 $mapper->fastmap( $sr_name, $seq_region_start, $seq_region_end,$seq_region_strand, $sr_cs );
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|
|
450 }
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|
|
451
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|
|
452 #skip features that map to gaps or coord system boundaries
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|
|
453 if(!defined($seq_region_id)) {
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|
|
454 $throw_away = $misc_feature_id;
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|
|
455 next FEATURE;
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|
|
456 }
|
|
|
457
|
|
|
458 #get a slice in the coord system we just mapped to
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|
|
459 # if ($asm_cs == $sr_cs ||
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|
|
460 # ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
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|
|
461 $slice = $slice_hash{"ID:".$seq_region_id} ||=
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|
|
462 $sa->fetch_by_seq_region_id($seq_region_id);
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|
|
463 # } else {
|
|
|
464 # $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||=
|
|
|
465 # $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef,
|
|
|
466 # $asm_cs_vers);
|
|
|
467 # }
|
|
|
468 }
|
|
|
469
|
|
|
470 #
|
|
|
471 # If a destination slice was provided convert the coords
|
|
|
472 # If the dest_slice starts at 1 and is foward strand, nothing needs doing
|
|
|
473 #
|
|
|
474 if ($dest_slice) {
|
|
|
475 if ($dest_slice_start != 1 || $dest_slice_strand != 1) {
|
|
|
476 if ($dest_slice_strand == 1) {
|
|
|
477 $seq_region_start = $seq_region_start - $dest_slice_start + 1;
|
|
|
478 $seq_region_end = $seq_region_end - $dest_slice_start + 1;
|
|
|
479 } else {
|
|
|
480 my $tmp_seq_region_start = $seq_region_start;
|
|
|
481 $seq_region_start = $dest_slice_end - $seq_region_end + 1;
|
|
|
482 $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1;
|
|
|
483 $seq_region_strand *= -1;
|
|
|
484 }
|
|
|
485 }
|
|
|
486 #throw away features off the end of the requested slice
|
|
|
487 if ($seq_region_end < 1 || $seq_region_start > $dest_slice_length ||
|
|
|
488 ( $dest_slice_sr_id ne $seq_region_id )) {
|
|
|
489 #flag this feature as one to throw away
|
|
|
490 $throw_away = $misc_feature_id;
|
|
|
491 next FEATURE;
|
|
|
492 }
|
|
|
493 $slice = $dest_slice;
|
|
|
494 }
|
|
|
495
|
|
|
496
|
|
|
497 if ($attrib_value && $attrib_type_code) {
|
|
|
498 my $attrib = Bio::EnsEMBL::Attribute->new
|
|
|
499 ( -CODE => $attrib_type_code,
|
|
|
500 -NAME => $attrib_type_name,
|
|
|
501 -DESC => $attrib_type_description,
|
|
|
502 -VALUE => $attrib_value
|
|
|
503 );
|
|
|
504 $feat_attribs = [$attrib];
|
|
|
505 $seen_attribs->{"$attrib_type_code:$attrib_value"} = 1;
|
|
|
506 }
|
|
|
507
|
|
|
508 $feat =
|
|
|
509 $self->_create_feature_fast( 'Bio::EnsEMBL::MiscFeature', {
|
|
|
510 'start' => $seq_region_start,
|
|
|
511 'end' => $seq_region_end,
|
|
|
512 'strand' => $seq_region_strand,
|
|
|
513 'slice' => $slice,
|
|
|
514 'adaptor' => $self,
|
|
|
515 'dbID' => $misc_feature_id,
|
|
|
516 'attributes' => $feat_attribs
|
|
|
517 } );
|
|
|
518
|
|
|
519 push @features, $feat;
|
|
|
520
|
|
|
521 if ($misc_set_id) {
|
|
|
522 #get the misc_set object
|
|
|
523 my $misc_set = $ms_hash{$misc_set_id} ||=
|
|
|
524 $msa->fetch_by_dbID($misc_set_id);
|
|
|
525 if ( ! exists $feat_misc_sets->{$misc_set->{'code'}} ) {
|
|
|
526 $feat->add_MiscSet( $misc_set );
|
|
|
527 $feat_misc_sets->{$misc_set->{'code'}} = $misc_set;
|
|
|
528 }
|
|
|
529 }
|
|
|
530 }
|
|
|
531 }
|
|
|
532
|
|
|
533 return \@features;
|
|
|
534 }
|
|
|
535
|
|
|
536
|
|
|
537
|
|
|
538 =head2 list_dbIDs
|
|
|
539
|
|
|
540 Arg [1] : none
|
|
|
541 Example : @feature_ids = @{$misc_feature_adaptor->list_dbIDs()};
|
|
|
542 Description: Gets an array of internal ids for all misc_features in the
|
|
|
543 current db
|
|
|
544 Arg[1] : <optional> int. not 0 for the ids to be sorted by the seq_region.
|
|
|
545 Returntype : list of ints
|
|
|
546 Exceptions : none
|
|
|
547 Caller : ?
|
|
|
548 Status : Stable
|
|
|
549
|
|
|
550 =cut
|
|
|
551
|
|
|
552 sub list_dbIDs {
|
|
|
553 my ($self,$ordered) = @_;
|
|
|
554
|
|
|
555 return $self->_list_dbIDs("misc_feature",undef,$ordered);
|
|
|
556 }
|
|
|
557
|
|
|
558
|
|
|
559 =head2 store
|
|
|
560
|
|
|
561 Arg [1] : list of Bio::EnsEMBL::MiscFeatures @misc_features
|
|
|
562 Example : $misc_feature_adaptor->store(@misc_features);
|
|
|
563 Description: Stores a list of MiscFeatures in this database. The stored
|
|
|
564 features will have their
|
|
|
565 Returntype : none
|
|
|
566 Exceptions : throw on invalid arguments
|
|
|
567 warning if misc feature is already stored in this database
|
|
|
568 throw if start/end/strand attribs are not valid
|
|
|
569 Caller : general
|
|
|
570 Status : Stable
|
|
|
571
|
|
|
572 =cut
|
|
|
573
|
|
|
574 sub store {
|
|
|
575 my $self = shift;
|
|
|
576 my @misc_features = @_;
|
|
|
577
|
|
|
578 my $db = $self->db();
|
|
|
579
|
|
|
580 my $feature_sth = $self->prepare
|
|
|
581 ("INSERT INTO misc_feature SET " .
|
|
|
582 " seq_region_id = ?, " .
|
|
|
583 " seq_region_start = ?, " .
|
|
|
584 " seq_region_end = ?, " .
|
|
|
585 " seq_region_strand = ?");
|
|
|
586
|
|
|
587 my $feature_set_sth = $self->prepare
|
|
|
588 ("INSERT IGNORE misc_feature_misc_set SET " .
|
|
|
589 " misc_feature_id = ?, " .
|
|
|
590 " misc_set_id = ?");
|
|
|
591
|
|
|
592 my $msa = $db->get_MiscSetAdaptor();
|
|
|
593 my $aa = $db->get_AttributeAdaptor();
|
|
|
594
|
|
|
595 FEATURE:
|
|
|
596 foreach my $mf (@misc_features) {
|
|
|
597 if(!ref($mf) || !$mf->isa('Bio::EnsEMBL::MiscFeature')) {
|
|
|
598 throw("List of MiscFeature arguments expeceted");
|
|
|
599 }
|
|
|
600
|
|
|
601 if($mf->is_stored($db)) {
|
|
|
602 warning("MiscFeature [" .$mf->dbID."] is already stored in database.");
|
|
|
603 next FEATURE;
|
|
|
604 }
|
|
|
605
|
|
|
606 # do some checking of the start/end and convert to seq_region coords
|
|
|
607 my $original = $mf;
|
|
|
608 my $seq_region_id;
|
|
|
609 ($mf, $seq_region_id) = $self->_pre_store($mf);
|
|
|
610
|
|
|
611 # store the actual MiscFeature
|
|
|
612 $feature_sth->bind_param(1,$seq_region_id,SQL_INTEGER);
|
|
|
613 $feature_sth->bind_param(2,$mf->start,SQL_INTEGER);
|
|
|
614 $feature_sth->bind_param(3,$mf->end,SQL_INTEGER);
|
|
|
615 $feature_sth->bind_param(4,$mf->strand,SQL_TINYINT);
|
|
|
616 $feature_sth->execute();
|
|
|
617
|
|
|
618 my $dbID = $feature_sth->{'mysql_insertid'};
|
|
|
619
|
|
|
620 $mf->dbID($dbID);
|
|
|
621 $mf->adaptor($self);
|
|
|
622
|
|
|
623 # store all the attributes
|
|
|
624 my $attribs = $mf->get_all_Attributes();
|
|
|
625 $aa->store_on_MiscFeature($mf, $attribs);
|
|
|
626
|
|
|
627 # store all the sets that have not been stored yet
|
|
|
628 my $sets = $mf->get_all_MiscSets();
|
|
|
629 foreach my $set (@$sets) {
|
|
|
630 $msa->store($set) if(!$set->is_stored($db));
|
|
|
631
|
|
|
632 # update the misc_feat_misc_set table to store the set relationship
|
|
|
633 $feature_set_sth->bind_param(1,$dbID,SQL_INTEGER);
|
|
|
634 $feature_set_sth->bind_param(2,$set->dbID,SQL_INTEGER);
|
|
|
635
|
|
|
636 $feature_set_sth->execute();
|
|
|
637 }
|
|
|
638 }
|
|
|
639
|
|
|
640 return;
|
|
|
641 }
|
|
|
642
|
|
|
643 1;
|
|
|
644
|
|
|
645
|
|
|
646
|
|
|
647
|
|
|
648
|
