Mercurial > repos > mahtabm > ensemb_rep_gvl
comparison variant_effect_predictor/Bio/Seq/LargeSeq.pm @ 0:2bc9b66ada89 draft default tip
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author | mahtabm |
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date | Thu, 11 Apr 2013 06:29:17 -0400 |
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1 # $Id: LargeSeq.pm,v 1.11 2002/10/22 07:38:40 lapp Exp $ | |
2 # | |
3 # BioPerl module for Bio::Seq::LargeSeq | |
4 # | |
5 # Cared for by Ewan Birney, Jason Stajich | |
6 # | |
7 # Copyright Ewan Birney, Jason Stajich | |
8 # | |
9 # You may distribute this module under the same terms as perl itself | |
10 | |
11 # POD documentation - main docs before the code | |
12 | |
13 =head1 NAME | |
14 | |
15 Bio::Seq::LargeSeq - SeqI compliant object that stores sequence as files in /tmp | |
16 | |
17 =head1 SYNOPSIS | |
18 | |
19 # normal primary seq usage | |
20 | |
21 =head1 DESCRIPTION | |
22 | |
23 This object stores a sequence as a series of files in a temporary | |
24 directory. The aim is to allow someone the ability to store very large | |
25 sequences (eg, E<gt> 100MBases) in a file system without running out of memory | |
26 (eg, on a 64 MB real memory machine!). | |
27 | |
28 Of course, to actually make use of this functionality, the programs | |
29 which use this object B<must> not call $primary_seq-E<gt>seq otherwise the | |
30 entire sequence will come out into memory and probably paste your | |
31 machine. However, calls $primary_seq-E<gt>subseq(10,100) will cause only | |
32 90 characters to be brought into real memory. | |
33 | |
34 =head1 FEEDBACK | |
35 | |
36 =head2 Mailing Lists | |
37 | |
38 User feedback is an integral part of the evolution of this | |
39 and other Bioperl modules. Send your comments and suggestions preferably | |
40 to one of the Bioperl mailing lists. | |
41 Your participation is much appreciated. | |
42 | |
43 bioperl-l@bioperl.org - General discussion | |
44 http://www.bioperl.org/MailList.html - About the mailing lists | |
45 | |
46 =head2 Reporting Bugs | |
47 | |
48 Report bugs to the Bioperl bug tracking system to help us keep track | |
49 the bugs and their resolution. | |
50 Bug reports can be submitted via email or the web: | |
51 | |
52 bioperl-bugs@bio.perl.org | |
53 http://bugzilla.bioperl.org/ | |
54 | |
55 =head1 AUTHOR - Ewan Birney | |
56 | |
57 Email birney@ebi.ac.uk | |
58 | |
59 =head1 APPENDIX | |
60 | |
61 The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ | |
62 | |
63 =cut | |
64 | |
65 | |
66 # Let the code begin... | |
67 | |
68 | |
69 package Bio::Seq::LargeSeq; | |
70 use vars qw($AUTOLOAD @ISA); | |
71 use strict; | |
72 | |
73 # Object preamble - inherits from Bio::Root::Objecttest 8, | |
74 | |
75 use Bio::Seq::LargePrimarySeq; | |
76 use Bio::Seq; | |
77 | |
78 @ISA = qw(Bio::Seq); | |
79 | |
80 | |
81 sub new { | |
82 my ($class, @args) = @_; | |
83 my $self = $class->SUPER::new(@args); | |
84 | |
85 my ($pseq) = $self->_rearrange([qw(PRIMARYSEQ)], @args); | |
86 | |
87 if( ! defined $pseq ) { | |
88 $pseq = new Bio::Seq::LargePrimarySeq(@args); | |
89 } | |
90 $self->primary_seq($pseq); | |
91 | |
92 return $self; | |
93 } | |
94 | |
95 | |
96 =head2 trunc | |
97 | |
98 Title : trunc | |
99 Usage : $subseq = $myseq->trunc(10,100); | |
100 Function: Provides a truncation of a sequence, | |
101 | |
102 Example : | |
103 Returns : a fresh Bio::SeqI object | |
104 Args : | |
105 | |
106 =cut | |
107 | |
108 sub trunc { | |
109 my ($self, $s, $e) = @_; | |
110 return new Bio::Seq::LargeSeq( | |
111 '-display_id' => $self->display_id, | |
112 '-accession_number' => $self->accession_number, | |
113 '-desc' => $self->desc, | |
114 '-alphabet' => $self->alphabet, | |
115 -primaryseq => | |
116 $self->primary_seq->trunc($s,$e)); | |
117 | |
118 } | |
119 | |
120 =head2 Bio::Seq::LargePrimarySeq methods | |
121 | |
122 =cut | |
123 | |
124 =head2 add_sequence_as_string | |
125 | |
126 Title : add_sequence_as_string | |
127 Usage : $seq->add_sequence_as_string("CATGAT"); | |
128 Function: Appends additional residues to an existing LargePrimarySeq object. | |
129 This allows one to build up a large sequence without storing | |
130 entire object in memory. | |
131 Returns : Current length of sequence | |
132 Args : string to append | |
133 | |
134 =cut | |
135 | |
136 sub add_sequence_as_string { | |
137 my ($self,$str) = @_; | |
138 return $self->primary_seq->add_sequence_as_string($str); | |
139 } | |
140 | |
141 1; |