Mercurial > repos > mahtabm > ensemb_rep_gvl
comparison variant_effect_predictor/Bio/Tools/Coil.pm @ 0:2bc9b66ada89 draft default tip
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author | mahtabm |
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date | Thu, 11 Apr 2013 06:29:17 -0400 |
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1 # Parser module for Coil Bio::Tools::Coil | |
2 # | |
3 # Based on the EnsEMBL module Bio::EnsEMBL::Pipeline::Runnable::Protein::Coil | |
4 # originally written by Marc Sohrmann (ms2@sanger.ac.uk) | |
5 # Written in BioPipe by Balamurugan Kumarasamy <savikalpa@fugu-sg.org> | |
6 # Cared for by the Fugu Informatics team (fuguteam@fugu-sg.org) | |
7 | |
8 # You may distribute this module under the same terms as perl itself | |
9 # | |
10 # POD documentation - main docs before the code | |
11 | |
12 =head1 NAME | |
13 | |
14 Bio::Tools::Coil | |
15 | |
16 =head1 SYNOPSIS | |
17 | |
18 use Bio::Tools::Coil | |
19 my $parser = new Bio::Tools::Coil(); | |
20 while( my $sp_feat = $parser->next_result($file) ) { | |
21 #do something | |
22 #eg | |
23 push @sp_feat, $sp_feat; | |
24 } | |
25 | |
26 =head1 DESCRIPTION | |
27 | |
28 Parser for Coil output | |
29 | |
30 =head1 FEEDBACK | |
31 | |
32 =head2 Mailing Lists | |
33 | |
34 User feedback is an integral part of the evolution of this and other | |
35 Bioperl modules. Send your comments and suggestions preferably to | |
36 the Bioperl mailing list. Your participation is much appreciated. | |
37 | |
38 bioperl-l@bioperl.org - General discussion | |
39 http://bioperl.org/MailList.shtml - About the mailing lists | |
40 | |
41 =head2 Reporting Bugs | |
42 | |
43 Report bugs to the Bioperl bug tracking system to help us keep track | |
44 of the bugs and their resolution. Bug reports can be submitted via | |
45 email or the web: | |
46 | |
47 bioperl-bugs@bio.perl.org | |
48 http://bugzilla.bioperl.org/ | |
49 | |
50 =head1 AUTHOR | |
51 | |
52 Based on the EnsEMBL module Bio::EnsEMBL::Pipeline::Runnable::Protein::Coil | |
53 originally written by Marc Sohrmann (ms2@sanger.ac.uk) | |
54 Written in BioPipe by Balamurugan Kumarasamy <savikalpa@fugu-sg.org> | |
55 Cared for by the Fugu Informatics team (fuguteam@fugu-sg.org) | |
56 | |
57 =head1 APPENDIX | |
58 | |
59 The rest of the documentation details each of the object methods. | |
60 Internal methods are usually preceded with a _ | |
61 | |
62 | |
63 =cut | |
64 | |
65 package Bio::Tools::Coil; | |
66 use vars qw(@ISA); | |
67 use strict; | |
68 | |
69 use Bio::Root::Root; | |
70 use Bio::SeqFeature::FeaturePair; | |
71 use Bio::Root::IO; | |
72 use Bio::SeqFeature::Generic; | |
73 @ISA = qw(Bio::Root::Root Bio::Root::IO); | |
74 | |
75 | |
76 | |
77 sub new { | |
78 my($class,@args) = @_; | |
79 | |
80 my $self = $class->SUPER::new(@args); | |
81 $self->_initialize_io(@args); | |
82 | |
83 return $self; | |
84 } | |
85 | |
86 =head2 parse_results | |
87 | |
88 Title : parse_results | |
89 Usage : obj->parse_results | |
90 Function: Parses the coil output. Automatically called by | |
91 next_result() if not yet done. | |
92 Example : | |
93 Returns : | |
94 | |
95 =cut | |
96 | |
97 sub parse_results { | |
98 my ($self,$resfile) = @_; | |
99 my $filehandle = $resfile; | |
100 my %result_hash =_read_fasta($filehandle);#bala no file handle | |
101 my @ids = keys %result_hash; | |
102 my @feats; | |
103 foreach my $id (keys %result_hash){ | |
104 my $pep = reverse ($result_hash{$id}); | |
105 my $count = my $switch = 0; | |
106 my ($start, $end); | |
107 while (my $aa = chop $pep) { | |
108 $count++; | |
109 if (!$switch && $aa eq "x") { | |
110 $start = $count; | |
111 $switch = 1; | |
112 } | |
113 elsif ($switch && $aa ne "x") { | |
114 $end = $count-1; | |
115 my (%feature); | |
116 $feature{name} = $id; | |
117 $feature{start} = $start; | |
118 $feature{end} = $end; | |
119 $feature{source} = "Coils"; | |
120 $feature{primary} = 'ncoils'; | |
121 ($feature{program}) = 'ncoils'; | |
122 $feature{logic_name} = 'Coils'; | |
123 my $new_feat = $self->create_feature (\%feature); | |
124 $self->_add_prediction($new_feat); | |
125 $switch = 0; | |
126 } | |
127 } | |
128 } | |
129 | |
130 $self->_predictions_parsed(1); | |
131 | |
132 } | |
133 =head2 next_result | |
134 | |
135 Title : next_result | |
136 Usage : while($feat = $coil->next_result($file)) { | |
137 # do something | |
138 } | |
139 Function: Returns the next protein feature of the coil output file | |
140 Returns : | |
141 Args : | |
142 | |
143 =cut | |
144 | |
145 sub next_result{ | |
146 | |
147 my ($self,$resfile) = @_; | |
148 my $gene; | |
149 | |
150 $self->parse_results($resfile) unless $self->_predictions_parsed(); | |
151 | |
152 $gene = $self->_result(); | |
153 | |
154 return $gene; | |
155 | |
156 } | |
157 | |
158 =head2 _result | |
159 | |
160 Title : _result | |
161 Usage : $feat = $obj->_result() | |
162 Function: internal | |
163 Example : | |
164 Returns : | |
165 | |
166 =cut | |
167 | |
168 sub _result{ | |
169 my ($self) = @_; | |
170 | |
171 return undef unless(exists($self->{'_feats'}) && @{$self->{'_feats'}}); | |
172 return shift(@{$self->{'_feats'}}); | |
173 | |
174 } | |
175 | |
176 =head2 _add_prediction | |
177 | |
178 Title : _add_prediction() | |
179 Usage : $obj->_add_prediction($feat) | |
180 Function: internal | |
181 Example : | |
182 Returns : | |
183 | |
184 =cut | |
185 | |
186 sub _add_prediction { | |
187 my ($self, $gene) = @_; | |
188 | |
189 if(! exists($self->{'_feats'})) { | |
190 $self->{'_feats'} = []; | |
191 } | |
192 push(@{$self->{'_feats'}}, $gene); | |
193 } | |
194 | |
195 =head2 _predictions_parsed | |
196 | |
197 Title : _predictions_parsed | |
198 Usage : $obj->_predictions_parsed | |
199 Function: internal | |
200 Example : | |
201 Returns : TRUE or FALSE | |
202 | |
203 =cut | |
204 | |
205 sub _predictions_parsed { | |
206 my ($self, $val) = @_; | |
207 | |
208 $self->{'_preds_parsed'} = $val if $val; | |
209 if(! exists($self->{'_preds_parsed'})) { | |
210 $self->{'_preds_parsed'} = 0; | |
211 } | |
212 return $self->{'_preds_parsed'}; | |
213 } | |
214 | |
215 | |
216 =head2 create_feature | |
217 | |
218 Title : create_feature | |
219 Usage : obj->create_feature(\%feature) | |
220 Function: Internal(not to be used directly) | |
221 Returns : | |
222 Args : | |
223 | |
224 | |
225 =cut | |
226 | |
227 sub create_feature { | |
228 my ($self, $feat) = @_; | |
229 | |
230 | |
231 # create feature object | |
232 my $feature = Bio::SeqFeature::Generic->new(-seq_id => $feat->{name}, | |
233 -start => $feat->{start}, | |
234 -end => $feat->{end}, | |
235 -score => $feat->{score}, | |
236 -source => $feat->{source}, | |
237 -primary => $feat->{primary}, | |
238 -logic_name => $feat->{logic_name}, | |
239 ); | |
240 $feature->add_tag_value('evalue',0); | |
241 $feature->add_tag_value('percent_id','NULL'); | |
242 $feature->add_tag_value("hid",$feat->{primary}); | |
243 | |
244 | |
245 return $feature; | |
246 | |
247 } | |
248 | |
249 =head2 _read_fasta | |
250 | |
251 Title : _read_fasta | |
252 Usage : obj->_read_fasta($file) | |
253 Function: Internal(not to be used directly) | |
254 Returns : | |
255 Args : | |
256 | |
257 | |
258 =cut | |
259 | |
260 sub _read_fasta { | |
261 local (*FILE) = @_; | |
262 my( $id , $seq , %name2seq);#bala | |
263 while (<FILE>) { | |
264 chomp;#bala | |
265 if (/^>(\S+)/) { | |
266 | |
267 my $new_id = $1; | |
268 if ($id) { | |
269 $name2seq{$id} = $seq; | |
270 } | |
271 $id = $new_id ; $seq = "" ; | |
272 } | |
273 elsif (eof) { | |
274 if ($id) { | |
275 $seq .= $_ ;#bala line instead of $_ | |
276 $name2seq{$id} = $seq; | |
277 } | |
278 } | |
279 else { | |
280 $seq .= $_ | |
281 } | |
282 } | |
283 return %name2seq; | |
284 } | |
285 | |
286 | |
287 | |
288 1; | |
289 | |
290 |