Mercurial > repos > mahtabm > ensemb_rep_gvl
comparison variant_effect_predictor/Bio/AlignIO/fasta.pm @ 0:2bc9b66ada89 draft default tip
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author | mahtabm |
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date | Thu, 11 Apr 2013 06:29:17 -0400 |
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1 # $Id: fasta.pm,v 1.11 2002/12/14 19:09:05 birney Exp $ | |
2 # | |
3 # BioPerl module for Bio::AlignIO::fasta | |
4 | |
5 # based on the Bio::SeqIO::fasta module | |
6 # by Ewan Birney <birney@sanger.ac.uk> | |
7 # and Lincoln Stein <lstein@cshl.org> | |
8 # | |
9 # and the SimpleAlign.pm module of Ewan Birney | |
10 # | |
11 # Copyright Peter Schattner | |
12 # | |
13 # You may distribute this module under the same terms as perl itself | |
14 # _history | |
15 # September 5, 2000 | |
16 # POD documentation - main docs before the code | |
17 | |
18 =head1 NAME | |
19 | |
20 Bio::AlignIO::fasta - FastA MSA Sequence input/output stream | |
21 | |
22 =head1 SYNOPSIS | |
23 | |
24 Do not use this module directly. Use it via the L<Bio::AlignIO> class. | |
25 | |
26 =head1 DESCRIPTION | |
27 | |
28 This object can transform L<Bio::SimpleAlign> objects to and from | |
29 fasta flat file databases. This is for the fasta sequence format NOT | |
30 FastA analysis program. To process the pairwise alignments from a | |
31 FastA (FastX, FastN, FastP, tFastA, etc) use the Bio::SearchIO module. | |
32 | |
33 =head1 FEEDBACK | |
34 | |
35 =head2 Reporting Bugs | |
36 | |
37 Report bugs to the Bioperl bug tracking system to help us keep track | |
38 the bugs and their resolution. Bug reports can be submitted via email | |
39 or the web: | |
40 | |
41 bioperl-bugs@bio.perl.org | |
42 http://bugzilla.bioperl.org/ | |
43 | |
44 =head1 AUTHORS - Peter Schattner | |
45 | |
46 Email: schattner@alum.mit.edu | |
47 | |
48 | |
49 =head1 APPENDIX | |
50 | |
51 The rest of the documentation details each of the object | |
52 methods. Internal methods are usually preceded with a _ | |
53 | |
54 =cut | |
55 | |
56 # Let the code begin... | |
57 | |
58 package Bio::AlignIO::fasta; | |
59 use vars qw(@ISA); | |
60 use strict; | |
61 | |
62 use Bio::AlignIO; | |
63 use Bio::SimpleAlign; | |
64 | |
65 @ISA = qw(Bio::AlignIO); | |
66 | |
67 | |
68 =head2 next_aln | |
69 | |
70 Title : next_aln | |
71 Usage : $aln = $stream->next_aln() | |
72 Function: returns the next alignment in the stream. | |
73 Returns : L<Bio::Align::AlignI> object - returns 0 on end of file | |
74 or on error | |
75 Args : NONE | |
76 | |
77 =cut | |
78 | |
79 sub next_aln { | |
80 my $self = shift; | |
81 my $entry; | |
82 my ($start,$end,$name,$seqname,$seq,$seqchar,$tempname,%align); | |
83 my $aln = Bio::SimpleAlign->new(); | |
84 | |
85 while(defined ($entry = $self->_readline)) { | |
86 if($entry =~ /^>(\S+)/ ) { | |
87 $tempname = $1; | |
88 if( defined $name ) { | |
89 # put away last name and sequence | |
90 | |
91 if( $name =~ /(\S+)\/(\d+)-(\d+)/ ) { | |
92 $seqname = $1; | |
93 $start = $2; | |
94 $end = $3; | |
95 } else { | |
96 $seqname=$name; | |
97 $start = 1; | |
98 $end = length($seqchar); #ps 9/6/00 | |
99 } | |
100 # print STDERR "Going to add with $seqchar $seqname\n"; | |
101 $seq = new Bio::LocatableSeq('-seq'=>$seqchar, | |
102 '-id'=>$seqname, | |
103 '-start'=>$start, | |
104 '-end'=>$end, | |
105 ); | |
106 | |
107 $aln->add_seq($seq); | |
108 } | |
109 $name = $tempname; | |
110 $seqchar = ""; | |
111 next; | |
112 } | |
113 $entry =~ s/[^A-Za-z\.\-]//g; | |
114 $seqchar .= $entry; | |
115 | |
116 } | |
117 # | |
118 # Next two lines are to silence warnings that | |
119 # otherwise occur at EOF when using <$fh> | |
120 | |
121 if (!defined $name) {$name="";} | |
122 if (!defined $seqchar) {$seqchar="";} | |
123 | |
124 # Put away last name and sequence | |
125 if( $name =~ /(\S+)\/(\d+)-(\d+)/ ) { | |
126 $seqname = $1; | |
127 $start = $2; | |
128 $end = $3; | |
129 } else { | |
130 $seqname=$name; | |
131 $start = 1; | |
132 $end = length($seqchar); #ps 9/6/00 | |
133 # $end = length($align{$name}); | |
134 } | |
135 | |
136 | |
137 # If $end <= 0, we have either reached the end of | |
138 # file in <> or we have encountered some other error | |
139 # | |
140 if ($end <= 0) { undef $aln; return $aln;} | |
141 | |
142 # This logic now also reads empty lines at the | |
143 # end of the file. Skip this is seqchar and seqname is null | |
144 if( length($seqchar) == 0 && length($seqname) == 0 ) { | |
145 # skip | |
146 } else { | |
147 # print STDERR "end to add with $seqchar $seqname\n"; | |
148 $seq = new Bio::LocatableSeq('-seq'=>$seqchar, | |
149 '-id'=>$seqname, | |
150 '-start'=>$start, | |
151 '-end'=>$end, | |
152 ); | |
153 | |
154 $aln->add_seq($seq); | |
155 } | |
156 | |
157 return $aln; | |
158 | |
159 } | |
160 | |
161 | |
162 =head2 write_aln | |
163 | |
164 Title : write_aln | |
165 Usage : $stream->write_aln(@aln) | |
166 Function: writes the $aln object into the stream in fasta format | |
167 Returns : 1 for success and 0 for error | |
168 Args : L<Bio::Align::AlignI> object | |
169 | |
170 | |
171 =cut | |
172 | |
173 sub write_aln { | |
174 my ($self,@aln) = @_; | |
175 my ($seq,$rseq,$name,$count,$length,$seqsub); | |
176 | |
177 foreach my $aln (@aln) { | |
178 if( ! $aln || ! $aln->isa('Bio::Align::AlignI') ) { | |
179 $self->warn("Must provide a Bio::Align::AlignI object when calling write_aln"); | |
180 next; | |
181 } | |
182 foreach $rseq ( $aln->each_seq() ) { | |
183 $name = $aln->displayname($rseq->get_nse()); | |
184 $seq = $rseq->seq(); | |
185 $self->_print (">$name\n") or return ; | |
186 $count =0; | |
187 $length = length($seq); | |
188 while( ($count * 60 ) < $length ) { | |
189 $seqsub = substr($seq,$count*60,60); | |
190 $self->_print ("$seqsub\n") or return ; | |
191 $count++; | |
192 } | |
193 } | |
194 } | |
195 $self->flush if $self->_flush_on_write && defined $self->_fh; | |
196 return 1; | |
197 } | |
198 | |
199 1; |